Rorug06G0296000

1-acyl-sn-glycerol-3-phosphate acyltransferase 4

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0008281
Physical Location & Seq
Forward (+)
44852746 .. 44855940
3195 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0296000.1

Sequence Viewer

Length: 3195 bp
ATGAAAGCAGTTCAGGAGACTCCTCTGCGTCGACTCCTCCTCCTCCATATTCTCTTGCTCTTTTTGCTTCCATTGGAGGCCACATCATCACAAAGAATACAGGCTGAAGCTCTCCTAAAATGGAAGAACAGCTTTACTTCTTCACCACCTTCTCTGAATTCATGGTCGCTCACAAACCTAAACAACCTATGCAACTGGACTGCCATTGTCTGCAACCGCAATACCAAAACAGTTTCTGAAATTCACCTTGCAAACTTCAACATCACTGCAGCACTAACACAGTTCAACTTCAACGAGTTTCTCGATCTCACCCATTTCAACCTCAATGGCAACAATTTCTCAGGGCCTATACCATCTGCCATTGGCAACCTCACCAAGCTCACCACCTTGGACTTGGGAAACAATAACTGGGAACGAGAAGTGCCTGCGGAAATGGGCAAGTTAACGGAGCTTCAGTTTCTAAGTCTCAACAACAACTATCTCAATGGTACCATTCCCTATCAGCTGGACAATCTCAAAAAGGTACAATATTTGCTTCTTGGAGGTAACCACTTAGACACTCCTGACTGGTCTAAATTTTCAGGCTTTCCTTTGTTGAGGTACCTTGATCTTTATGGCAACAATTTAGATTCAGAATTCCCAGAATTCATATCTGAATGTAGGAACTTGACATTCCTGGATTTGTCTCAAAATGCTTTGACTGGCCACATACCAGAAGTGGTATTTACCAACCTGGTCAAGCTTGAATCTCTCAATCTCACCAATAACCGATTCCAAGGACCGTTGCCATATAACTTTCCCAACCTTAAGCACCTTTATTTGGAAGCAAACAACTTTAGTGGTCCAATGCCTGAAGATATTGGTTTGATATCTGGTCTTCAATTTATTAACCTTTATAACAATTCCTTTGAAGGCAAAATCCCATCCTCTGTAGGCCAACTCAGGGAGCTTCAGCACCTTGATTTTTCATCAAATTCATTAAACTCTTCATTCCTTTCTGAGCTTGGTTCTTGTACCAACCTCACTTTCTTGGCCTTGGCTTTAAATAAACTCAGTGGGGAATTGCCTCTCTCCTTGTCAAAATTGACCAACTTGGTGAAGTTGGGTTTATCTGATAATCTATTTACTGGGCCAATCTTGCCTTCTCTGGTCTCCAATTGGACTGAGATGATCTCTTTGCAACTCAAAAACAACAGCTTCAATGGGAATATTCCACCAGAAATTGGTCTGTTGACAAAACTTGATATCCTCTGGCTGAGCGAAAACAATTTCTCTGGATCAATTCCCTCAGAGATAGGAAACTTGAAAGTTTTGAAATATTTACGTCTTTGGGAAAACCAGCTCTCAGGGCCGATTCCTATGAAATTTTGGAACCTCACAAATCTTCAGGTCCTTGATATAGGAGCCAATAGCTTCAACGGGAATATTCCAGCAGAAATTGGCCTGTTGACAAAACTTCATATCCTCTGGCTGGGCGAAAACAATTTCTCTGGATCAATTCCCTCAGAGATAGGAAATTTGAAAGATTCGAGAGAATTGTACCTTGGGGGAAACCAGCTCTCAGGGCCGATTCCTATGAAATTCTGGAACCTCACAAATCTTCAGATGCTTGGTATGGAAGACAATAGCTTCAATGGGAATATTCCACCAGAATTTGGCCTGTTGACAAAACTTGATTGCATCTGGCTGGGCGAAAACAACTTCTCTGGCCCAATTCCCTTGGAGATGGCAAACTTGAAAGATCTGACAACTTTTAATCTTTCGAGAAACTACCTCTCCGGCCCAATTCCCCCAGAGATTGGAAACATGAGTTCCATGGTTAACTTTGATGTCAGCACAAACCAACTTGAAGGGGAGTTGCCGAAAACTATTTCTCACCTCAGAAAACTAGAGTGGTTTGCCGTTTACATTAATAGGTTTTCGGGGACCATTCCCAGTGATTTCGGGAAGTATAGTCCTAACTTGACATTTGTTAGTTTCGCATGGAACAGCTTCTCTGGAGAATTGCCGCCAGGGTTGTGTAGTGGATTTTCTTTGAAGCTTTTCTTAGTGCAGTTCAACGACTTCACAGGGCCATTACCTGATTGCTTGAGAAATTGTTCTGCATTATCTAGAGTCAGACTTGACGAGAATCAATTCACCGGAAACATTATCAATGCATTTGGTGTTCATCCCAGTCTTGCAGTAATTTATCTTAGTAACAACAAGTTTGTTGGTCAACTCTCGCCACAGTGGGCAGAATGCAAGAACATCACTGATATGCGGATGGAGGGAAATAGAATTTCTGGTCAAATCCCACCTGAACTTGGGCAGTTGACACAGTTGTGGTATCTAACCCTAAGCTCTAATGAATTCAGTGGGGAGTTTCCAGTTTCTATTGGGAATCTAAGCTTGTTGTTCACTCTTAATCTCAGCAGGAACCACTTGACGGGAACTATCCCTCATGTTCTAGGCAAATTGACTAAGCTTGAGATTCTTGATTTGTCTGATAATAATTTTACAGGGGTATTAACAGTCGACCCTGGGATGTTTGAGAGCTTAACAAGCTTGAACATAAGCCATAACAAATTATCAGGTGGAATACCTGAGGAGATTGGTAATCTGCACCTGCGGTACGTGTTGGACCTAAGCAGCAATTCATTCTCGGGATCACTACCATTAAACCTTGCCAAGCTTATAATGCTGGAGATTCTTAATTTCTCGCATAACCAACTCTCCGGAAACATCCCATCAACATATTCCAGAATGGTTAGTCTTCTCGGCATTGATTTTTCTTACAACAACTTGACAGGTCCAATCCCAACCGGTGGCATTTTCCAAAAGAAGCTTCCGAATAATGCCTTTGTTGGAAATGGTGGTTTGTGTGGAGAAACAGAGGGACTAACTCCATGCACTTCGGGTCCCAAAAGGTCCAACAAGAAAATTCTTGTTGCCGTCCTTGTTCCCATTTGTGGTTTATTGCTGATGGTGACCATATCTGCTGTAGTCCTTAAGTTCTGCAAGAAATTAAAGCTTGGAGAAATCGATCATCCTAAGGTGTCTAAAAGTTTTGATTCAATTATTTGGGGAAGACATGGTAAACTGACATTTTCTGAAATCATCACTGCCACCGAGAACTTTGATGAAAAGTACCTCATTGGAAGAGGAGGTTTTGGAAGTGTCTACAAAGCTATGTTGGACAGAGGCAGAAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00561 ko00564 ko01100 ko01110 map00561 map00564 map01100 map01110
Pfam Domains
Protein Families
Acyltransf_C Acyltransferase

Protein Analysis

1064

Amino Acids

117.93

Weight (kDa)

5.94

Isoelectric Point (pI)

30.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 34 - 72 2e-06 Leucine rich repeat N-terminal domain
LRR_14 PF23598 99 - 179 1.1e-07 Leucine-rich repeat region
LRR_8 PF13855 150 - 209 4.2e-07 Leucine rich repeat
LRR_8 PF13855 198 - 258 1.9e-08 Leucine rich repeat
LRR_14 PF23598 430 - 513 1.9e-09 Leucine-rich repeat region
LRR_14 PF23598 768 - 851 7.5e-08 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 897, 2678
AarI CACCTGC 1 cut(s) 2616
Acc36I ACCTGC 1 cut(s) 2616
Acc65I GGTACC 2 cut(s) 488, 600
AccB1I GGYRCC 2 cut(s) 488, 600
AccB7I CCANNNNNTGG 4 cut(s) 1223, 1655, 1799, 2807
AccI GTMKAC 3 cut(s) 31, 2517, 3162
AccIII TCCGGA 1 cut(s) 2717
AciI CCGC 5 cut(s) 217, 428, 2009, 2263, 2611
AclWI GGATC 3 cut(s) 1285, 1501, 2656
AcoI YGGCCR 1 cut(s) 703
AcuI CTGAAG 6 cut(s) 126, 437, 873, 935, 1370, 1586
AfaI GTAC 7 cut(s) 490, 525, 602, 1015, 1541, 2615, 3131
AflII CTTAAG 2 cut(s) 806, 2990
AflIII ACRYGT 1 cut(s) 2616
AgeI ACCGGT 1 cut(s) 2804
AjnI CCWGG 4 cut(s) 675, 732, 2011, 2521
AleI CACNNNNGTG 1 cut(s) 2323
Alw26I GTCTC 4 cut(s) 11, 470, 690, 1156
AlwI GGATC 3 cut(s) 1285, 1501, 2656
AlwNI CAGNNNCTG 1 cut(s) 236
Ama87I CYCGRG 1 cut(s) 2644
Aor13HI TCCGGA 1 cut(s) 2717
ApeKI GCWGC 2 cut(s) 269, 2631
AseI ATTAAT 1 cut(s) 1911
AsiGI ACCGGT 1 cut(s) 2804
Asp700I GAANNNNTTC 4 cut(s) 1991, 2042, 2098, 2135
Asp718I GGTACC 2 cut(s) 488, 600
AvaI CYCGRG 1 cut(s) 2644
AvaII GGWCC 8 cut(s) 779, 842, 1390, 1926, 2623, 2792, 2900, 2910
AxyI CCTNAGG 2 cut(s) 2586, 3033
BalI TGGCCA 1 cut(s) 705
BanI GGYRCC 2 cut(s) 488, 600
BbsI GAAGAC 4 cut(s) 869, 1626, 2747, 3076
BbvI GCAGC 2 cut(s) 281, 2643
BccI CCATC 6 cut(s) 361, 931, 1720, 2260, 2737, 2959
BceAI ACGGC 2 cut(s) 1886, 2918
BciT130I CCWGG 4 cut(s) 677, 734, 2013, 2523
BcoDI GTCTC 4 cut(s) 11, 470, 690, 1156
BfaI CTAG 3 cut(s) 1889, 2112, 2450
BfmI CTRYAG 3 cut(s) 267, 930, 2982
BfrI CTTAAG 2 cut(s) 806, 2990
BfuAI ACCTGC 1 cut(s) 2616
BglII AGATCT 1 cut(s) 1741
BisI GCNGC 3 cut(s) 270, 2009, 2632
BlpI GCTNAGC 1 cut(s) 1256
BlsI GCNGC 3 cut(s) 271, 2010, 2633
Bme1390I CCNGG 4 cut(s) 677, 734, 2013, 2523
Bme18I GGWCC 8 cut(s) 779, 842, 1390, 1926, 2623, 2792, 2900, 2910
BmeT110I CYCGRG 1 cut(s) 2644
BmiI GGNNCC 9 cut(s) 490, 602, 1373, 1405, 1589, 1927, 2420, 2901, 2902
BmrFI CCNGG 4 cut(s) 677, 734, 2013, 2523
BmrI ACTGGG 4 cut(s) 418, 1137, 1929, 2169
BmsI GCATC 2 cut(s) 1596, 1689
BmuI ACTGGG 4 cut(s) 418, 1137, 1929, 2169
BpiI GAAGAC 4 cut(s) 869, 1626, 2747, 3076
BplI GAGNNNNNCTC 2 cut(s) 1157, 1189
BpmI CTGGAG 2 cut(s) 2019, 2705
Bpu10I CCTNAGC 2 cut(s) 2339, 2627
Bpu1102I GCTNAGC 1 cut(s) 1256
BpuEI CTTGAG 2 cut(s) 2110, 2489
Bsa29I ATCGAT 1 cut(s) 3024
BsaAI YACGTR 1 cut(s) 2617
BsaI GGTCTC 1 cut(s) 1156
BsaJI CCNNGG 9 cut(s) 387, 775, 1035, 1543, 1719, 1815, 2012, 2521, 2522
BsaWI WCCGGW 3 cut(s) 2141, 2717, 2804
BsaXI ACNNNNNCTCC 8 cut(s) 24, 54, 440, 470, 1760, 1790, 2699, 2729
Bse118I RCCGGY 1 cut(s) 2804
Bse1I ACTGG 8 cut(s) 200, 413, 572, 706, 1132, 1935, 2175, 2369
Bse21I CCTNAGG 2 cut(s) 2586, 3033
BseAI TCCGGA 1 cut(s) 2717
BseBI CCWGG 4 cut(s) 677, 734, 2013, 2523
BseCI ATCGAT 1 cut(s) 3024
BseDI CCNNGG 9 cut(s) 387, 775, 1035, 1543, 1719, 1815, 2012, 2521, 2522
BseGI GGATG 6 cut(s) 923, 2170, 2271, 2532, 2724, 3028
BseNI ACTGG 8 cut(s) 200, 413, 572, 706, 1132, 1935, 2175, 2369
BseRI GAGGAG 6 cut(s) 12, 26, 29, 32, 2603, 3159
BseXI GCAGC 2 cut(s) 281, 2643
BseYI CCCAGC 2 cut(s) 1471, 1687
BsgI GTGCAG 2 cut(s) 2072, 2588
BshNI GGYRCC 2 cut(s) 488, 600
BshTI ACCGGT 1 cut(s) 2804
BshVI ATCGAT 1 cut(s) 3024
BsiHKCI CYCGRG 1 cut(s) 2644
BsiSI CCGG 4 cut(s) 1779, 2142, 2718, 2805
BslFI GGGAC 3 cut(s) 1939, 2886, 2892
BsmAI GTCTC 4 cut(s) 11, 470, 690, 1156
BsmFI GGGAC 3 cut(s) 1939, 2886, 2892
BsmI GAATGC 1 cut(s) 2246
Bso31I GGTCTC 1 cut(s) 1156
BsoBI CYCGRG 1 cut(s) 2644
Bsp13I TCCGGA 1 cut(s) 2717
Bsp143I GATC 8 cut(s) 304, 607, 1170, 1277, 1493, 1741, 2648, 3025
Bsp1720I GCTNAGC 1 cut(s) 1256
Bsp19I CCATGG 1 cut(s) 1815
BspACI CCGC 5 cut(s) 217, 428, 2009, 2263, 2611
BspDI ATCGAT 1 cut(s) 3024
BspEI TCCGGA 1 cut(s) 2717
BspLI GGNNCC 9 cut(s) 490, 602, 1373, 1405, 1589, 1927, 2420, 2901, 2902
BspMAI CTGCAG 1 cut(s) 271
BspMI ACCTGC 1 cut(s) 2616
BspPI GGATC 3 cut(s) 1285, 1501, 2656
BspT107I GGYRCC 2 cut(s) 488, 600
BspTI CTTAAG 2 cut(s) 806, 2990
BspTNI GGTCTC 1 cut(s) 1156
BsrFI RCCGGY 1 cut(s) 2804
BsrI ACTGG 8 cut(s) 200, 413, 572, 706, 1132, 1935, 2175, 2369
BssAI RCCGGY 1 cut(s) 2804
BssECI CCNNGG 9 cut(s) 387, 775, 1035, 1543, 1719, 1815, 2012, 2521, 2522
BssMI GATC 8 cut(s) 304, 607, 1170, 1277, 1493, 1741, 2648, 3025
BssT1I CCWWGG 6 cut(s) 387, 775, 1035, 1543, 1719, 1815
Bst2UI CCWGG 4 cut(s) 677, 734, 2013, 2523
Bst4CI ACNGT 6 cut(s) 232, 282, 783, 2232, 2322, 2515
Bst6I CTCTTC 2 cut(s) 991, 3136
BstAFI CTTAAG 2 cut(s) 806, 2990
BstBAI YACGTR 1 cut(s) 2617
BstC8I GCNNGC 1 cut(s) 426
BstDSI CCRYGG 1 cut(s) 1815
BstEII GGTNACC 2 cut(s) 545, 2968
BstF5I GGATG 6 cut(s) 923, 2170, 2271, 2532, 2724, 3028
BstKTI GATC 8 cut(s) 307, 610, 1173, 1280, 1496, 1744, 2651, 3028
BstMAI GTCTC 4 cut(s) 11, 470, 690, 1156
BstMBI GATC 8 cut(s) 304, 607, 1170, 1277, 1493, 1741, 2648, 3025
BstMWI GCNNNNNNNGC 6 cut(s) 64, 1138, 1348, 1564, 2544, 2680
BstNI CCWGG 4 cut(s) 677, 734, 2013, 2523
BstPI GGTNACC 2 cut(s) 545, 2968
BstSCI CCNGG 4 cut(s) 675, 732, 2011, 2521
BstSFI CTRYAG 3 cut(s) 267, 930, 2982
BstV1I GCAGC 2 cut(s) 281, 2643
BstV2I GAAGAC 4 cut(s) 869, 1626, 2747, 3076
BstX2I RGATCY 1 cut(s) 1741
BstYI RGATCY 1 cut(s) 1741
Bsu15I ATCGAT 1 cut(s) 3024
Bsu36I CCTNAGG 2 cut(s) 2586, 3033
BsuTUI ATCGAT 1 cut(s) 3024
BtgI CCRYGG 1 cut(s) 1815
BtsCI GGATG 6 cut(s) 923, 2170, 2271, 2532, 2724, 3028
BtsI GCAGTG 2 cut(s) 264, 3102
BtsIMutI CAGTG 7 cut(s) 264, 1060, 1942, 2237, 2253, 2362, 3102
BveI ACCTGC 1 cut(s) 2616
Cac8I GCNNGC 1 cut(s) 426
CaiI CAGNNNCTG 1 cut(s) 236
Cfr10I RCCGGY 1 cut(s) 2804
ClaI ATCGAT 1 cut(s) 3024
CseI GACGC 1 cut(s) 17
CsiI ACCWGGT 1 cut(s) 732
Csp6I GTAC 7 cut(s) 489, 524, 601, 1014, 1540, 2614, 3130
CspAI ACCGGT 1 cut(s) 2804
CspCI CAANNNNNGTGG 2 cut(s) 820, 855
CviAII CATG 7 cut(s) 162, 1807, 1816, 1983, 2444, 2889, 3074
CviQI GTAC 7 cut(s) 489, 524, 601, 1014, 1540, 2614, 3130
DpnI GATC 8 cut(s) 306, 609, 1172, 1279, 1495, 1743, 2650, 3027
DpnII GATC 8 cut(s) 304, 607, 1170, 1277, 1493, 1741, 2648, 3025
DraI TTTAAA 1 cut(s) 1044
EaeI YGGCCR 1 cut(s) 703
Eam1104I CTCTTC 2 cut(s) 991, 3136
EarI CTCTTC 2 cut(s) 991, 3136
Eco130I CCWWGG 6 cut(s) 387, 775, 1035, 1543, 1719, 1815
Eco31I GGTCTC 1 cut(s) 1156
Eco32I GATATC 2 cut(s) 870, 1246
Eco47I GGWCC 8 cut(s) 779, 842, 1390, 1926, 2623, 2792, 2900, 2910
Eco57I CTGAAG 6 cut(s) 126, 437, 873, 935, 1370, 1586
Eco81I CCTNAGG 2 cut(s) 2586, 3033
Eco88I CYCGRG 1 cut(s) 2644
Eco91I GGTNACC 2 cut(s) 545, 2968
EcoO109I RGGNCCY 3 cut(s) 344, 1390, 2900
EcoO65I GGTNACC 2 cut(s) 545, 2968
EcoRI GAATTC 4 cut(s) 157, 635, 644, 2351
EcoRII CCWGG 4 cut(s) 675, 732, 2011, 2521
EcoRV GATATC 2 cut(s) 870, 1246
EcoT14I CCWWGG 6 cut(s) 387, 775, 1035, 1543, 1719, 1815
EcoT22I ATGCAT 1 cut(s) 2161
ErhI CCWWGG 6 cut(s) 387, 775, 1035, 1543, 1719, 1815
FaeI CATG 7 cut(s) 165, 1810, 1819, 1986, 2447, 2892, 3077
FalI AAGNNNNNCTT 4 cut(s) 116, 148, 2030, 2062
FaqI GGGAC 3 cut(s) 1939, 2886, 2892
FatI CATG 7 cut(s) 161, 1806, 1815, 1982, 2443, 2888, 3073
FblI GTMKAC 3 cut(s) 31, 2517, 3162
Fnu4HI GCNGC 3 cut(s) 270, 2009, 2632
FokI GGATG 6 cut(s) 910, 2157, 2278, 2539, 2711, 3015
Fsp4HI GCNGC 3 cut(s) 270, 2009, 2632
FspBI CTAG 3 cut(s) 1889, 2112, 2450
GluI GCNGC 3 cut(s) 270, 2009, 2632
GsaI CCCAGC 2 cut(s) 1475, 1691
GsuI CTGGAG 2 cut(s) 2019, 2705
HapII CCGG 4 cut(s) 1779, 2142, 2718, 2805
HgaI GACGC 1 cut(s) 17
Hin1II CATG 7 cut(s) 165, 1810, 1819, 1986, 2447, 2892, 3077
HincII GTYRAC 9 cut(s) 32, 444, 1233, 1449, 1665, 1822, 2219, 2316, 2518
HindII GTYRAC 9 cut(s) 32, 444, 1233, 1449, 1665, 1822, 2219, 2316, 2518
HindIII AAGCTT 8 cut(s) 740, 2039, 2389, 2465, 2545, 2672, 2825, 3011
HpaI GTTAAC 2 cut(s) 444, 1822
HpaII CCGG 4 cut(s) 1779, 2142, 2718, 2805
Hpy99I CGWCG 1 cut(s) 33
HpyAV CCTTC 4 cut(s) 159, 905, 1152, 1844
HpyCH4III ACNGT 6 cut(s) 232, 282, 783, 2232, 2322, 2515
HpyCH4IV ACGT 2 cut(s) 1324, 2616
HpyF10VI GCNNNNNNNGC 6 cut(s) 64, 1138, 1348, 1564, 2544, 2680
HpySE526I ACGT 2 cut(s) 1324, 2616
Hsp92II CATG 7 cut(s) 165, 1810, 1819, 1986, 2447, 2892, 3077
KflI GGGWCCC 1 cut(s) 2900
Kpn2I TCCGGA 1 cut(s) 2717
KpnI GGTACC 2 cut(s) 492, 604
KspAI GTTAAC 2 cut(s) 444, 1822
Kzo9I GATC 8 cut(s) 304, 607, 1170, 1277, 1493, 1741, 2648, 3025
LmnI GCTCC 3 cut(s) 448, 946, 1403
Lsp1109I GCAGC 2 cut(s) 281, 2643
LweI GCATC 2 cut(s) 1596, 1689
MabI ACCWGGT 1 cut(s) 732
MaeI CTAG 3 cut(s) 1889, 2112, 2450
MaeII ACGT 2 cut(s) 1324, 2616
MaeIII GTNAC 3 cut(s) 545, 2198, 2968
MalI GATC 8 cut(s) 306, 609, 1172, 1279, 1495, 1743, 2650, 3027
MboI GATC 8 cut(s) 304, 607, 1170, 1277, 1493, 1741, 2648, 3025
MfeI CAATTG 1 cut(s) 1156
MflI RGATCY 1 cut(s) 1741
MlsI TGGCCA 1 cut(s) 705
MluNI TGGCCA 1 cut(s) 705
MlyI GAGTC 3 cut(s) 13, 27, 2124
MmeI TCCRAC 4 cut(s) 2601, 2827, 2937, 3156
Mox20I TGGCCA 1 cut(s) 705
Mph1103I ATGCAT 1 cut(s) 2161
MroI TCCGGA 1 cut(s) 2717
MroXI GAANNNNTTC 4 cut(s) 1991, 2042, 2098, 2135
MscI TGGCCA 1 cut(s) 705
MslI CAYNNNNRTG 2 cut(s) 2258, 2323
Msp20I TGGCCA 1 cut(s) 705
MspA1I CMGCKG 1 cut(s) 505
MspCI CTTAAG 2 cut(s) 806, 2990
MspI CCGG 4 cut(s) 1779, 2142, 2718, 2805
MspR9I CCNGG 4 cut(s) 677, 734, 2013, 2523
MunI CAATTG 1 cut(s) 1156
Mva1269I GAATGC 1 cut(s) 2246
MvaI CCWGG 4 cut(s) 677, 734, 2013, 2523
MwoI GCNNNNNNNGC 6 cut(s) 64, 1138, 1348, 1564, 2544, 2680
NcoI CCATGG 1 cut(s) 1815
NdeII GATC 8 cut(s) 304, 607, 1170, 1277, 1493, 1741, 2648, 3025
NlaIII CATG 7 cut(s) 165, 1810, 1819, 1986, 2447, 2892, 3077
NlaIV GGNNCC 9 cut(s) 490, 602, 1373, 1405, 1589, 1927, 2420, 2901, 2902
NmeAIII GCCGAG 1 cut(s) 2739
NmuCI GTSAC 1 cut(s) 2968
NsiI ATGCAT 1 cut(s) 2161
OliI CACNNNNGTG 1 cut(s) 2323
PaqCI CACCTGC 1 cut(s) 2616
PasI CCCWGGG 1 cut(s) 2522
PcsI WCGNNNNNNNCGW 1 cut(s) 300
PctI GAATGC 1 cut(s) 2246
PdmI GAANNNNTTC 4 cut(s) 1991, 2042, 2098, 2135
PflMI CCANNNNNTGG 4 cut(s) 1223, 1655, 1799, 2807
PfoI TCCNGGA 1 cut(s) 675
PinAI ACCGGT 1 cut(s) 2804
PkrI GCNGC 3 cut(s) 271, 2010, 2633
PleI GAGTC 3 cut(s) 13, 27, 2123
PpsI GAGTC 3 cut(s) 13, 27, 2123
Ppu21I YACGTR 1 cut(s) 2617
PpuMI RGGWCCY 2 cut(s) 1390, 2900
PshBI ATTAAT 1 cut(s) 1911
PsiI TTATAA 2 cut(s) 897, 2678
Psp5II RGGWCCY 2 cut(s) 1390, 2900
Psp6I CCWGG 4 cut(s) 675, 732, 2011, 2521
PspEI GGTNACC 2 cut(s) 545, 2968
PspFI CCCAGC 2 cut(s) 1471, 1687
PspGI CCWGG 4 cut(s) 675, 732, 2011, 2521
PspN4I GGNNCC 9 cut(s) 490, 602, 1373, 1405, 1589, 1927, 2420, 2901, 2902
PspPPI RGGWCCY 2 cut(s) 1390, 2900
PstI CTGCAG 1 cut(s) 271
PstNI CAGNNNCTG 1 cut(s) 236
PsuI RGATCY 1 cut(s) 1741
PvuII CAGCTG 1 cut(s) 505
RsaI GTAC 7 cut(s) 490, 525, 602, 1015, 1541, 2615, 3131
RsaNI GTAC 7 cut(s) 489, 524, 601, 1014, 1540, 2614, 3130
RseI CAYNNNNRTG 2 cut(s) 2258, 2323
SalI GTCGAC 2 cut(s) 30, 2516
SatI GCNGC 3 cut(s) 270, 2009, 2632
Sau3AI GATC 8 cut(s) 304, 607, 1170, 1277, 1493, 1741, 2648, 3025
SchI GAGTC 3 cut(s) 13, 27, 2124
ScrFI CCNGG 4 cut(s) 677, 734, 2013, 2523
SexAI ACCWGGT 1 cut(s) 732
SfaNI GCATC 2 cut(s) 1596, 1689
SfcI CTRYAG 3 cut(s) 267, 930, 2982
SinI GGWCC 8 cut(s) 779, 842, 1390, 1926, 2623, 2792, 2900, 2910
SmiMI CAYNNNNRTG 2 cut(s) 2258, 2323
SmlI CTYRAG 4 cut(s) 806, 2089, 2468, 2990
SmoI CTYRAG 4 cut(s) 806, 2089, 2468, 2990
SsiI CCGC 5 cut(s) 217, 428, 2009, 2263, 2611
SspI AATATT 5 cut(s) 530, 1210, 1319, 1426, 1642
SspMI CTAG 3 cut(s) 1889, 2112, 2450
StyD4I CCNGG 4 cut(s) 675, 732, 2011, 2521
StyI CCWWGG 6 cut(s) 387, 775, 1035, 1543, 1719, 1815
TaaI ACNGT 6 cut(s) 232, 282, 783, 2232, 2322, 2515
TaiI ACGT 2 cut(s) 1327, 2619
TaqI TCGA 6 cut(s) 31, 303, 1529, 1763, 2517, 3024
TauI GCSGC 1 cut(s) 2011
TscAI CASTG 7 cut(s) 271, 1060, 1942, 2237, 2260, 2362, 3109
TseFI GTSAC 1 cut(s) 2968
TseI GCWGC 2 cut(s) 269, 2631
Tsp45I GTSAC 1 cut(s) 2968
TspGWI ACGGA 1 cut(s) 461
TspRI CASTG 7 cut(s) 271, 1060, 1942, 2237, 2260, 2362, 3109
Van91I CCANNNNNTGG 4 cut(s) 1223, 1655, 1799, 2807
Vha464I CTTAAG 2 cut(s) 806, 2990
VpaK11BI GGWCC 8 cut(s) 779, 842, 1390, 1926, 2623, 2792, 2900, 2910
VspI ATTAAT 1 cut(s) 1911
XbaI TCTAGA 1 cut(s) 2111
XcmI CCANNNNNNNNNTGG 1 cut(s) 391
XmiI GTMKAC 3 cut(s) 31, 2517, 3162
XmnI GAANNNNTTC 4 cut(s) 1991, 2042, 2098, 2135
XspI CTAG 3 cut(s) 1889, 2112, 2450
Zsp2I ATGCAT 1 cut(s) 2161
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.