Rh6AG408700

1-acyl-sn-glycerol-3-phosphate acyltransferase 4

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0008281
Physical Location & Seq
Forward (+)
60532041 .. 60534440
2400 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG408700.1

Sequence Viewer

Length: 1269 bp
ATGAGATCTTCACCGAAAAAGATTGCCATGAGCCTTCAGGCCAGAGAGAGCGCCAGTCCCAGACGGGTAGCGGCAACCCCAGAAGAATGCTCCATTGAGGACGGTTCGTGGAAGGAGGTGGACGTTTGCAGTCCCTTAAAATCTGATAGTAAACTGAAGCACAAGCCCTTGAATGCTTTTAGAGTTGTAAGGGGTGTTCTATGTTTGGTGGTGTTTCTTTCCACTGCTTTCACCATTCTAGTCTGTTTTGCGCCGGTGGTAGCTGTTTTGTTGCGACTTTTTAGCATACATTACAGCAGAAAAGCAACATCCTTTCTATTTGCTAGGTGGCTGGCTCTCTGGCCCTTTCTATTTGAAAAGATCAACGGGACGAACGTGGTTTTTTCGGGCGATACTGTCCCACCTAGAGAGCGCATTTTGATTATTGCGAATCATAAAACTGAGGTGGATTGGATGTACTTGTGGGATCTTGCATTGCGAAAAGGGTCCCTCGGATATGTTAAATATGTGCTCAAGAGCAGCTTGATGAAGCTGCCTGTTTTCGGTTGGGGGTTCCACGTTCTGGAGTTCATTCCTTTGAAGAGGAAGTGGGAAGTTGATGAACCAATTCTCCGGAAAATGCTATTAAGTTTTGCTGATCCTCGGGACCCTCTCTGGCTTTCTATTTTTCCTGAAGGAACTGATTACGATGAAGAAAAATGCAAAAAGAGCCAGACATTTGCTGCTGAAAATGGACTTCCTGTGCTGTCACATGTGCTACTTCCAAGGACTAAAGGTTTTTGTGCTTGCTTAGAAGCCCTAAGAGGTTCTTTGGATGCAGTTTATGACTTGACAATTGCATACAAGAATCAATGCCCTTCGTTTATGGACAATGCATTTGGTGTAGATCCTTCAGAAGTACACATTCATGTTCGGCGTATCCCAATGGAAGATATCCCAGAGTCTAGCACAGATGCTGCATCTTGGTTAGTGGACACATTCCATCTCAAGGACAACATGCTGTCAGATTTCCATACTCGAGGCCATTTTCCTAATGAAGGAGGAGAAGAAGACATTTCTGCGTTCAAGGGTTTGGTAAATTTTATGTTGATACTTATTTTGACAGTGGTTATCATTTACCTTACCATTTTTTCATCGGTCTGGTTTAAAATATACATCGGTTTATCATGTGCATACCTTGCTTCCGCAACATATTTTGAAATCCAACCGGCGCCAGTATTAGACTTTGTTAAAGCAACATTGGTTTGCAGTAGACTCAGATGTGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00561 ko00564 ko01100 ko01110 map00561 map00564 map01100 map01110
Pfam Domains
Protein Families
Acyltransf_C Acyltransferase

Protein Analysis

422

Amino Acids

47.85

Weight (kDa)

7.95

Isoelectric Point (pI)

48.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Acyltransferase PF01553 127 - 251 3e-16 Acyltransferase
Acyltransf_C PF16076 294 - 362 5.8e-18 Acyltransferase C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1210
AccB7I CCANNNNNTGG 1 cut(s) 562
AccI GTMKAC 1 cut(s) 1252
AccIII TCCGGA 1 cut(s) 612
AciI CCGC 2 cut(s) 71, 1185
AclWI GGATC 3 cut(s) 474, 632, 881
AcsI RAATTY 1 cut(s) 1078
AcuI CTGAAG 4 cut(s) 20, 176, 693, 876
AcyI GRCGYC 1 cut(s) 1211
AfaI GTAC 2 cut(s) 458, 900
AfiI CCNNNNNNNGG 4 cut(s) 542, 562, 988, 1037
AflIII ACRYGT 1 cut(s) 751
AgsI TTSAA 5 cut(s) 172, 356, 580, 1066, 1199
AloI GAACNNNNNNTCC 2 cut(s) 594, 626
AluBI AGCT 3 cut(s) 263, 522, 532
AluI AGCT 3 cut(s) 263, 522, 532
Alw21I GWGCWC 1 cut(s) 513
AlwI GGATC 3 cut(s) 474, 632, 881
AlwNI CAGNNNCTG 1 cut(s) 956
Ama87I CYCGRG 2 cut(s) 642, 1017
Aor13HI TCCGGA 1 cut(s) 612
AoxI GGCC 3 cut(s) 39, 341, 1021
ApeKI GCWGC 4 cut(s) 519, 532, 722, 956
ApoI RAATTY 1 cut(s) 1078
ArsI GACNNNNNNTTYG 2 cut(s) 860, 892
Asp700I GAANNNNTTC 1 cut(s) 606
AspLEI GCGC 4 cut(s) 53, 253, 414, 1213
AspS9I GGNCC 3 cut(s) 342, 486, 646
AsuHPI GGTGA 2 cut(s) 3, 223
AvaI CYCGRG 2 cut(s) 642, 1017
AvaII GGWCC 2 cut(s) 486, 646
BanI GGYRCC 1 cut(s) 1210
BbsI GAAGAC 1 cut(s) 1056
Bbv12I GWGCWC 1 cut(s) 513
BbvI GCAGC 4 cut(s) 519, 531, 709, 943
BccI CCATC 1 cut(s) 990
BciVI GTATCC 1 cut(s) 929
BfaI CTAG 4 cut(s) 239, 324, 405, 945
BfoI RGCGCY 2 cut(s) 54, 1214
BfuI GTATCC 1 cut(s) 929
BglII AGATCT 1 cut(s) 5
BisI GCNGC 5 cut(s) 72, 520, 533, 723, 957
BlsI GCNGC 5 cut(s) 73, 521, 534, 724, 958
Bme18I GGWCC 2 cut(s) 486, 646
BmeT110I CYCGRG 2 cut(s) 642, 1017
BmgT120I GGNCC 3 cut(s) 342, 486, 646
BmiI GGNNCC 6 cut(s) 487, 488, 554, 647, 648, 1212
BmsI GCATC 3 cut(s) 805, 943, 968
BpiI GAAGAC 1 cut(s) 1056
BpmI CTGGAG 1 cut(s) 584
BpuEI CTTGAG 2 cut(s) 497, 971
BsaHI GRCGYC 1 cut(s) 1211
BsaJI CCNNGG 3 cut(s) 490, 641, 764
BsaWI WCCGGW 1 cut(s) 612
BsaXI ACNNNNNCTCC 2 cut(s) 594, 624
Bsc4I CCNNNNNNNGG 4 cut(s) 542, 562, 988, 1037
Bse118I RCCGGY 2 cut(s) 253, 1207
Bse1I ACTGG 2 cut(s) 54, 1214
Bse3DI GCAATG 1 cut(s) 473
BseAI TCCGGA 1 cut(s) 612
BseDI CCNNGG 3 cut(s) 490, 641, 764
BseGI GGATG 3 cut(s) 308, 459, 820
BseLI CCNNNNNNNGG 4 cut(s) 542, 562, 988, 1037
BseMI GCAATG 1 cut(s) 473
BseMII CTCAG 1 cut(s) 432
BseNI ACTGG 2 cut(s) 54, 1214
BseRI GAGGAG 1 cut(s) 1056
BseXI GCAGC 4 cut(s) 519, 531, 709, 943
BshFI GGCC 3 cut(s) 41, 343, 1023
BshNI GGYRCC 1 cut(s) 1210
BsiHKAI GWGCWC 1 cut(s) 513
BsiHKCI CYCGRG 2 cut(s) 642, 1017
BsiSI CCGG 3 cut(s) 254, 613, 1208
BslFI GGGAC 6 cut(s) 42, 117, 382, 383, 472, 659
BslI CCNNNNNNNGG 4 cut(s) 542, 562, 988, 1037
BsmFI GGGAC 6 cut(s) 42, 117, 382, 383, 472, 659
BsmI GAATGC 2 cut(s) 92, 178
BsnI GGCC 3 cut(s) 41, 343, 1023
BsoBI CYCGRG 2 cut(s) 642, 1017
Bsp1286I GDGCHC 1 cut(s) 513
Bsp13I TCCGGA 1 cut(s) 612
Bsp143I GATC 5 cut(s) 5, 360, 466, 637, 886
BspACI CCGC 2 cut(s) 71, 1185
BspANI GGCC 3 cut(s) 41, 343, 1023
BspCNI CTCAG 2 cut(s) 433, 1269
BspEI TCCGGA 1 cut(s) 612
BspLI GGNNCC 6 cut(s) 487, 488, 554, 647, 648, 1212
BspPI GGATC 3 cut(s) 474, 632, 881
BspT107I GGYRCC 1 cut(s) 1210
BsrDI GCAATG 1 cut(s) 473
BsrFI RCCGGY 2 cut(s) 253, 1207
BsrI ACTGG 2 cut(s) 54, 1214
BssAI RCCGGY 2 cut(s) 253, 1207
BssECI CCNNGG 3 cut(s) 490, 641, 764
BssMI GATC 5 cut(s) 5, 360, 466, 637, 886
BssNI GRCGYC 1 cut(s) 1211
BssT1I CCWWGG 1 cut(s) 764
Bst4CI ACNGT 3 cut(s) 104, 397, 1105
Bst6I CTCTTC 1 cut(s) 575
BstACI GRCGYC 1 cut(s) 1211
BstAPI GCANNNNNTGC 1 cut(s) 1178
BstC8I GCNNGC 2 cut(s) 333, 787
BstDEI CTNAG 4 cut(s) 441, 790, 800, 1256
BstENI CCTNNNNNAGG 1 cut(s) 1035
BstF5I GGATG 3 cut(s) 308, 459, 820
BstH2I RGCGCY 2 cut(s) 54, 1214
BstHHI GCGC 4 cut(s) 53, 253, 414, 1213
BstKTI GATC 5 cut(s) 8, 363, 469, 640, 889
BstMBI GATC 5 cut(s) 5, 360, 466, 637, 886
BstMWI GCNNNNNNNGC 2 cut(s) 708, 1178
BstNSI RCATGY 2 cut(s) 755, 1000
BstV1I GCAGC 4 cut(s) 519, 531, 709, 943
BstV2I GAAGAC 1 cut(s) 1056
BstX2I RGATCY 3 cut(s) 5, 466, 886
BstYI RGATCY 3 cut(s) 5, 466, 886
BsuI GTATCC 1 cut(s) 929
BsuRI GGCC 3 cut(s) 41, 343, 1023
BtsCI GGATG 3 cut(s) 308, 459, 820
BtsI GCAGTG 1 cut(s) 222
BtsIMutI CAGTG 2 cut(s) 222, 1110
Cac8I GCNNGC 2 cut(s) 333, 787
CaiI CAGNNNCTG 1 cut(s) 956
CfoI GCGC 4 cut(s) 53, 253, 414, 1213
Cfr10I RCCGGY 2 cut(s) 253, 1207
Cfr13I GGNCC 3 cut(s) 342, 486, 646
Csp6I GTAC 2 cut(s) 457, 899
CviAII CATG 5 cut(s) 28, 752, 908, 997, 1167
CviQI GTAC 2 cut(s) 457, 899
DdeI CTNAG 4 cut(s) 441, 790, 800, 1256
DinI GGCGCC 1 cut(s) 1212
DpnI GATC 5 cut(s) 7, 362, 468, 639, 888
DpnII GATC 5 cut(s) 5, 360, 466, 637, 886
DraI TTTAAA 1 cut(s) 1147
Eam1104I CTCTTC 1 cut(s) 575
EarI CTCTTC 1 cut(s) 575
Eco130I CCWWGG 1 cut(s) 764
Eco32I GATATC 1 cut(s) 934
Eco47I GGWCC 2 cut(s) 486, 646
Eco57I CTGAAG 4 cut(s) 20, 176, 693, 876
Eco88I CYCGRG 2 cut(s) 642, 1017
EcoNI CCTNNNNNAGG 1 cut(s) 1035
EcoO109I RGGNCCY 2 cut(s) 486, 646
EcoRV GATATC 1 cut(s) 934
EcoT14I CCWWGG 1 cut(s) 764
EcoT22I ATGCAT 1 cut(s) 877
EgeI GGCGCC 1 cut(s) 1212
EheI GGCGCC 1 cut(s) 1212
ErhI CCWWGG 1 cut(s) 764
FaeI CATG 5 cut(s) 31, 755, 911, 1000, 1170
FalI AAGNNNNNCTT 4 cut(s) 506, 538, 793, 825
FaqI GGGAC 6 cut(s) 42, 117, 382, 383, 472, 659
FatI CATG 5 cut(s) 27, 751, 907, 996, 1166
FblI GTMKAC 1 cut(s) 1252
Fnu4HI GCNGC 5 cut(s) 72, 520, 533, 723, 957
FokI GGATG 3 cut(s) 295, 466, 827
Fsp4HI GCNGC 5 cut(s) 72, 520, 533, 723, 957
FspBI CTAG 4 cut(s) 239, 324, 405, 945
GlaI GCGC 4 cut(s) 52, 252, 413, 1212
GluI GCNGC 5 cut(s) 72, 520, 533, 723, 957
GsuI CTGGAG 1 cut(s) 584
HaeII RGCGCY 2 cut(s) 54, 1214
HaeIII GGCC 3 cut(s) 41, 343, 1023
HapII CCGG 3 cut(s) 254, 613, 1208
HhaI GCGC 4 cut(s) 53, 253, 414, 1213
Hin1I GRCGYC 1 cut(s) 1211
Hin1II CATG 5 cut(s) 31, 755, 911, 1000, 1170
Hin6I GCGC 4 cut(s) 51, 251, 412, 1211
HinP1I GCGC 4 cut(s) 51, 251, 412, 1211
HinfI GANTC 4 cut(s) 430, 847, 941, 1254
HpaII CCGG 3 cut(s) 254, 613, 1208
HphI GGTGA 2 cut(s) 3, 223
Hpy166II GTNNAC 5 cut(s) 121, 152, 901, 973, 1253
Hpy188I TCNGA 5 cut(s) 145, 494, 895, 1006, 1259
Hpy188III TCNNGA 5 cut(s) 514, 563, 613, 644, 671
Hpy8I GTNNAC 5 cut(s) 121, 152, 901, 973, 1253
HpyAV CCTTC 6 cut(s) 44, 106, 668, 867, 900, 1031
HpyCH4III ACNGT 3 cut(s) 104, 397, 1105
HpyCH4IV ACGT 3 cut(s) 123, 375, 558
HpyCH4V TGCA 9 cut(s) 129, 473, 702, 818, 839, 875, 959, 1172, 1248
HpyF10VI GCNNNNNNNGC 2 cut(s) 708, 1178
HpyF3I CTNAG 4 cut(s) 441, 790, 800, 1256
HpySE526I ACGT 3 cut(s) 123, 375, 558
Hsp92I GRCGYC 1 cut(s) 1211
Hsp92II CATG 5 cut(s) 31, 755, 911, 1000, 1170
HspAI GCGC 4 cut(s) 51, 251, 412, 1211
KasI GGCGCC 1 cut(s) 1210
KflI GGGWCCC 2 cut(s) 486, 646
Kpn2I TCCGGA 1 cut(s) 612
Kzo9I GATC 5 cut(s) 5, 360, 466, 637, 886
LmnI GCTCC 1 cut(s) 95
Lsp1109I GCAGC 4 cut(s) 519, 531, 709, 943
LweI GCATC 3 cut(s) 805, 943, 968
MaeI CTAG 4 cut(s) 239, 324, 405, 945
MaeII ACGT 3 cut(s) 123, 375, 558
MaeIII GTNAC 1 cut(s) 747
MalI GATC 5 cut(s) 7, 362, 468, 639, 888
MboI GATC 5 cut(s) 5, 360, 466, 637, 886
MboII GAAGA 6 cut(s) 95, 592, 704, 941, 1058, 1061
MfeI CAATTG 1 cut(s) 834
MflI RGATCY 3 cut(s) 5, 466, 886
MhlI GDGCHC 1 cut(s) 513
MluCI AATT 3 cut(s) 606, 834, 1078
Mly113I GGCGCC 1 cut(s) 1211
MlyI GAGTC 2 cut(s) 950, 1248
MmeI TCCRAC 1 cut(s) 1228
Mph1103I ATGCAT 1 cut(s) 877
MroI TCCGGA 1 cut(s) 612
MroXI GAANNNNTTC 1 cut(s) 606
MseI TTAA 5 cut(s) 137, 501, 626, 1146, 1230
MslI CAYNNNNRTG 1 cut(s) 906
MspI CCGG 3 cut(s) 254, 613, 1208
MunI CAATTG 1 cut(s) 834
Mva1269I GAATGC 2 cut(s) 92, 178
MwoI GCNNNNNNNGC 2 cut(s) 708, 1178
NarI GGCGCC 1 cut(s) 1211
NdeII GATC 5 cut(s) 5, 360, 466, 637, 886
NlaIII CATG 5 cut(s) 31, 755, 911, 1000, 1170
NlaIV GGNNCC 6 cut(s) 487, 488, 554, 647, 648, 1212
NmuCI GTSAC 1 cut(s) 747
NsiI ATGCAT 1 cut(s) 877
NspI RCATGY 2 cut(s) 755, 1000
PaeR7I CTCGAG 1 cut(s) 1017
PciI ACATGT 1 cut(s) 751
PcsI WCGNNNNNNNCGW 1 cut(s) 372
PctI GAATGC 2 cut(s) 92, 178
PdmI GAANNNNTTC 1 cut(s) 606
PfeI GAWTC 2 cut(s) 430, 847
PflMI CCANNNNNTGG 1 cut(s) 562
PkrI GCNGC 5 cut(s) 73, 521, 534, 724, 958
PleI GAGTC 2 cut(s) 949, 1248
PluTI GGCGCC 1 cut(s) 1214
PpsI GAGTC 2 cut(s) 949, 1248
PpuMI RGGWCCY 2 cut(s) 486, 646
PscI ACATGT 1 cut(s) 751
Psp5II RGGWCCY 2 cut(s) 486, 646
PspN4I GGNNCC 6 cut(s) 487, 488, 554, 647, 648, 1212
PspPI GGNCC 3 cut(s) 342, 486, 646
PspPPI RGGWCCY 2 cut(s) 486, 646
PspXI VCTCGAGB 1 cut(s) 1017
PsrI GAACNNNNNNTAC 2 cut(s) 180, 212
PstNI CAGNNNCTG 1 cut(s) 956
PsuI RGATCY 3 cut(s) 5, 466, 886
RsaI GTAC 2 cut(s) 458, 900
RsaNI GTAC 2 cut(s) 457, 899
RseI CAYNNNNRTG 1 cut(s) 906
SaqAI TTAA 5 cut(s) 137, 501, 626, 1146, 1230
SatI GCNGC 5 cut(s) 72, 520, 533, 723, 957
Sau3AI GATC 5 cut(s) 5, 360, 466, 637, 886
Sau96I GGNCC 3 cut(s) 342, 486, 646
SchI GAGTC 2 cut(s) 950, 1248
SduI GDGCHC 1 cut(s) 513
SfaNI GCATC 3 cut(s) 805, 943, 968
SfoI GGCGCC 1 cut(s) 1212
Sfr274I CTCGAG 1 cut(s) 1017
SgrAI CRCCGGYG 1 cut(s) 253
SinI GGWCC 2 cut(s) 486, 646
SlaI CTCGAG 1 cut(s) 1017
SmiMI CAYNNNNRTG 1 cut(s) 906
SmlI CTYRAG 3 cut(s) 512, 986, 1017
SmoI CTYRAG 3 cut(s) 512, 986, 1017
Sse9I AATT 3 cut(s) 606, 834, 1078
SsiI CCGC 2 cut(s) 71, 1185
SspDI GGCGCC 1 cut(s) 1210
SspMI CTAG 4 cut(s) 239, 324, 405, 945
StyI CCWWGG 1 cut(s) 764
TaaI ACNGT 3 cut(s) 104, 397, 1105
TaiI ACGT 3 cut(s) 126, 378, 561
TaqI TCGA 1 cut(s) 1018
TaqII GACCGA 1 cut(s) 1126
TasI AATT 3 cut(s) 606, 834, 1078
TatI WGTACW 2 cut(s) 456, 898
TauI GCSGC 1 cut(s) 74
TfiI GAWTC 2 cut(s) 430, 847
Tru1I TTAA 5 cut(s) 137, 501, 626, 1146, 1230
Tru9I TTAA 5 cut(s) 137, 501, 626, 1146, 1230
TscAI CASTG 2 cut(s) 229, 1110
TseFI GTSAC 1 cut(s) 747
TseI GCWGC 4 cut(s) 519, 532, 722, 956
Tsp45I GTSAC 1 cut(s) 747
TspDTI ATGAA 7 cut(s) 542, 559, 615, 705, 896, 1050, 1122
TspRI CASTG 2 cut(s) 229, 1110
Van91I CCANNNNNTGG 1 cut(s) 562
VpaK11BI GGWCC 2 cut(s) 486, 646
XagI CCTNNNNNAGG 1 cut(s) 1035
XapI RAATTY 1 cut(s) 1078
XceI RCATGY 2 cut(s) 755, 1000
XhoI CTCGAG 1 cut(s) 1017
XmiI GTMKAC 1 cut(s) 1252
XmnI GAANNNNTTC 1 cut(s) 606
XspI CTAG 4 cut(s) 239, 324, 405, 945
Zsp2I ATGCAT 1 cut(s) 877
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.