pycom15g08160
NAC Family

inactive heme oxygenase 2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0009205
Physical Location & Seq
Forward (+)
5068546 .. 5070193
1648 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1068 bp
ATGATTAATTTGAAATACCGTTCACAGGTGCTCGTGACACTGTTTCTAACACAATTATCTTCTGCAATGCAAATTATGCAATGCGCAACGATGTCGTTCATGGAGAAAACCGTCTCACCATCAATCGCTAATTTCGTCTTTCCACACCCCCATTCGTCGAATGCCTCAGCAATTTCAACCAGAAAGTTTCGTCGTCGAGCCCCGCCGCTCCGCTGCTCCGACTCGAGCGCCGCCGCTTCCATGTCTCTTTCTCCGCCGCCCATTTCGACGGCGAACAACGGTACGGCGCCGCCCGTGGTGAGGAGGAGGATGAGGTACCGGAAGCAGTACCCAGGGGAGAGCAAAGGCATCACTGAGGAGATGAGGTTCGTCGCCATGAAGCTCCGCAACATCAACGGCAAGAAATTGAACATCAACAGTAACAATTCCTCTTCTGATAACGATGACGGTGATGGTAAAAATGACGATAATGCCCCCGTCGAGGACAACACCTCGGAATCGGATGCCGACGGCGACGGGGGTGAAACGGAGACTTGGCGGCCGAGCTTGGAAGGGTTTATCAAGTACTTGGTGGACAGCAAGCTCGTCTTCGACACCGTCGAGCGCATTGTCGATGAATCGAACGACGTCGCTTATGCATATTTTAGGAAGACTGGATTGGAGAGATCGGAGAGTCTCTCGGAAGATCTCGAATGGTTTAAGCAGCGGGGCAATGTGATTCCAGAACCCAGTAATCCAGGAGTTTCATATGCTAAGTATTTGGAGGGGCTGGCAGAGGAGTCTGCCCCATTGTTCCTCTGCCACTTCTACAACATCTACTTTTCACATATAGCTGGTGGGCAGGTCATTGCAAGACAGGTATCTGAGAAGTTGCTGGAAGGAAGGGAGTTGGCATTTTGCGCATGGGAAGAGGACGTGCAGGAGTTATTGAAAGGTGTTCGTGAGAAGCTCAACAAGCTCGGAGAGCACTGGACGCGAGACGACAAAAACAAATGCTTAAAAGAAACAACAAAATCATTTCGGTATTTGGGGCAGATAGTTCGATTGATCATCTTGGAGCTCAAGTAA

Protein Analysis

356

Amino Acids

40.05

Weight (kDa)

6.57

Isoelectric Point (pI)

48.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Heme_oxygenase PF01126 186 - 348 5.3e-10 Heme oxygenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0009205)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G26550 AT2G26550 AT2G26550 AT2G26550
fragaria_vesca FvH4_2g34841 FvH4_2g34841 FvH4_2g34841 FvH4_2g34841 FvH4_2g34841
malus_domestica MD15G1086700.v1.1
prunus_persica Prupe.1G440300_v2.0.a1
pyrus_communis pycom15g08160
rosa_chinensis RchiOBHm_Chr6g0307211
rosa_laevigata RLG00000010727
rosa_multiflora Rmu_sc0041325.1_g000001
rosa_roxburghii Rroxscaffold_7G00161080
rosa_rugosa Rorug06G0357700
rosa_samantha Rh6AG468300 Rh6BG432200 Rh6CG483500 Rh6DG470100
rosa_wichuraiana Rw6G040800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 630
Acc16I TGCGCA 2 cut(s) 85, 901
Acc36I ACCTGC 1 cut(s) 832
Acc65I GGTACC 1 cut(s) 315
AccB1I GGYRCC 2 cut(s) 286, 315
AccBSI CCGCTC 1 cut(s) 208
AccII CGCG 1 cut(s) 976
AcoI YGGCCR 1 cut(s) 539
AcyI GRCGYC 2 cut(s) 287, 627
AfaI GTAC 4 cut(s) 283, 317, 329, 566
AfiI CCNNNNNNNGG 3 cut(s) 25, 300, 481
AgsI TTSAA 4 cut(s) 13, 177, 409, 931
AjiI CACGTC 1 cut(s) 916
AjnI CCWGG 2 cut(s) 331, 736
AjuI GAANNNNNNNTTGG 2 cut(s) 641, 673
AloI GAACNNNNNNTCC 2 cut(s) 350, 382
AluBI AGCT 7 cut(s) 382, 546, 583, 833, 949, 958, 1060
AluI AGCT 7 cut(s) 382, 546, 583, 833, 949, 958, 1060
Alw21I GWGCWC 3 cut(s) 33, 969, 1062
Alw26I GTCTC 5 cut(s) 118, 249, 524, 680, 972
Ama87I CYCGRG 1 cut(s) 223
AoxI GGCC 1 cut(s) 539
ApeKI GCWGC 2 cut(s) 213, 703
AseI ATTAAT 1 cut(s) 6
Asp718I GGTACC 1 cut(s) 315
AspLEI GCGC 5 cut(s) 86, 230, 289, 606, 902
AsuHPI GGTGA 4 cut(s) 108, 310, 461, 533
AvaI CYCGRG 1 cut(s) 223
BanI GGYRCC 2 cut(s) 286, 315
BanII GRGCYC 2 cut(s) 202, 1062
BauI CACGAG 1 cut(s) 32
BbsI GAAGAC 2 cut(s) 580, 656
Bbv12I GWGCWC 3 cut(s) 33, 969, 1062
BbvCI CCTCAGC 1 cut(s) 166
BbvI GCAGC 2 cut(s) 200, 715
BccI CCATC 2 cut(s) 127, 446
BceAI ACGGC 4 cut(s) 285, 300, 412, 526
BcgI CGANNNNNNTGC 4 cut(s) 75, 109, 1022, 1056
BciT130I CCWGG 2 cut(s) 333, 738
BclI TGATCA 1 cut(s) 1047
BcoDI GTCTC 5 cut(s) 118, 249, 524, 680, 972
BfoI RGCGCY 2 cut(s) 231, 290
BfuAI ACCTGC 1 cut(s) 832
BglII AGATCT 1 cut(s) 685
BisI GCNGC 8 cut(s) 206, 214, 231, 234, 257, 290, 539, 704
BlsI GCNGC 8 cut(s) 207, 215, 232, 235, 258, 291, 540, 705
BmcAI AGTACT 1 cut(s) 566
Bme1390I CCNGG 2 cut(s) 333, 738
BmeT110I CYCGRG 1 cut(s) 223
BmgBI CACGTC 1 cut(s) 916
BmiI GGNNCC 2 cut(s) 288, 317
BmrFI CCNGG 2 cut(s) 333, 738
BmrI ACTGGG 1 cut(s) 723
BmsI GCATC 2 cut(s) 357, 493
BmuI ACTGGG 1 cut(s) 723
BpiI GAAGAC 2 cut(s) 580, 656
BplI GAGNNNNNCTC 2 cut(s) 662, 694
Bpu10I CCTNAGC 1 cut(s) 166
BpuEI CTTGAG 1 cut(s) 1046
BsaHI GRCGYC 2 cut(s) 287, 627
BsaJI CCNNGG 4 cut(s) 294, 331, 332, 492
BsaWI WCCGGW 1 cut(s) 318
BsaXI ACNNNNNCTCC 3 cut(s) 350, 380, 1049
Bsc4I CCNNNNNNNGG 3 cut(s) 25, 300, 481
Bse1I ACTGG 3 cut(s) 658, 729, 974
Bse3DI GCAATG 4 cut(s) 72, 86, 718, 846
BseBI CCWGG 2 cut(s) 333, 738
BseDI CCNNGG 4 cut(s) 294, 331, 332, 492
BseGI GGATG 2 cut(s) 315, 508
BseLI CCNNNNNNNGG 3 cut(s) 25, 300, 481
BseMI GCAATG 4 cut(s) 72, 86, 718, 846
BseMII CTCAG 3 cut(s) 180, 345, 855
BseNI ACTGG 3 cut(s) 658, 729, 974
BseRI GAGGAG 4 cut(s) 316, 319, 371, 791
BseX3I CGGCCG 1 cut(s) 539
BseXI GCAGC 2 cut(s) 200, 715
BsgI GTGCAG 1 cut(s) 938
Bsh1236I CGCG 1 cut(s) 976
Bsh1285I CGRYCG 1 cut(s) 542
BshFI GGCC 1 cut(s) 541
BshNI GGYRCC 2 cut(s) 286, 315
BsiEI CGRYCG 1 cut(s) 542
BsiHKAI GWGCWC 3 cut(s) 33, 969, 1062
BsiHKCI CYCGRG 1 cut(s) 223
BsiSI CCGG 1 cut(s) 319
BslI CCNNNNNNNGG 3 cut(s) 25, 300, 481
BsmAI GTCTC 5 cut(s) 118, 249, 524, 680, 972
BsmBI CGTCTC 2 cut(s) 118, 972
BsmI GAATGC 1 cut(s) 166
BsnI GGCC 1 cut(s) 541
BsoBI CYCGRG 1 cut(s) 223
Bsp1286I GDGCHC 4 cut(s) 33, 202, 969, 1062
Bsp143I GATC 3 cut(s) 665, 685, 1047
BspANI GGCC 1 cut(s) 541
BspCNI CTCAG 3 cut(s) 179, 346, 856
BspFNI CGCG 1 cut(s) 976
BspLI GGNNCC 2 cut(s) 288, 317
BspMI ACCTGC 1 cut(s) 832
BspT107I GGYRCC 2 cut(s) 286, 315
BsrBI CCGCTC 1 cut(s) 208
BsrDI GCAATG 4 cut(s) 72, 86, 718, 846
BsrI ACTGG 3 cut(s) 658, 729, 974
BssECI CCNNGG 4 cut(s) 294, 331, 332, 492
BssMI GATC 3 cut(s) 665, 685, 1047
BssNI GRCGYC 2 cut(s) 287, 627
BssSI CACGAG 1 cut(s) 32
Bst2BI CACGAG 1 cut(s) 32
Bst2UI CCWGG 2 cut(s) 333, 738
Bst4CI ACNGT 7 cut(s) 20, 42, 112, 281, 419, 449, 598
Bst6I CTCTTC 2 cut(s) 436, 903
BstACI GRCGYC 2 cut(s) 287, 627
BstAPI GCANNNNNTGC 1 cut(s) 76
BstC8I GCNNGC 2 cut(s) 581, 771
BstDEI CTNAG 4 cut(s) 166, 354, 753, 864
BstDSI CCRYGG 1 cut(s) 294
BstF5I GGATG 2 cut(s) 315, 508
BstFNI CGCG 1 cut(s) 976
BstH2I RGCGCY 2 cut(s) 231, 290
BstHHI GCGC 5 cut(s) 86, 230, 289, 606, 902
BstKTI GATC 3 cut(s) 668, 688, 1050
BstMAI GTCTC 5 cut(s) 118, 249, 524, 680, 972
BstMBI GATC 3 cut(s) 665, 685, 1047
BstMCI CGRYCG 1 cut(s) 542
BstMWI GCNNNNNNNGC 5 cut(s) 76, 899, 955, 964, 973
BstNI CCWGG 2 cut(s) 333, 738
BstSCI CCNGG 2 cut(s) 331, 736
BstUI CGCG 1 cut(s) 976
BstV1I GCAGC 2 cut(s) 200, 715
BstV2I GAAGAC 2 cut(s) 580, 656
BstX2I RGATCY 1 cut(s) 685
BstYI RGATCY 1 cut(s) 685
BstZI CGGCCG 1 cut(s) 539
BsuRI GGCC 1 cut(s) 541
BtgI CCRYGG 1 cut(s) 294
BtrI CACGTC 1 cut(s) 916
BtsCI GGATG 2 cut(s) 315, 508
BtsIMutI CAGTG 3 cut(s) 38, 351, 967
BveI ACCTGC 1 cut(s) 832
Cac8I GCNNGC 2 cut(s) 581, 771
CfoI GCGC 5 cut(s) 86, 230, 289, 606, 902
CseI GACGC 1 cut(s) 982
Csp6I GTAC 4 cut(s) 282, 316, 328, 565
CviAII CATG 4 cut(s) 100, 241, 376, 903
CviQI GTAC 4 cut(s) 282, 316, 328, 565
DdeI CTNAG 4 cut(s) 166, 354, 753, 864
DinI GGCGCC 1 cut(s) 288
DpnI GATC 3 cut(s) 667, 687, 1049
DpnII GATC 3 cut(s) 665, 685, 1047
EaeI YGGCCR 1 cut(s) 539
EagI CGGCCG 1 cut(s) 539
Eam1104I CTCTTC 2 cut(s) 436, 903
EarI CTCTTC 2 cut(s) 436, 903
EciI GGCGGA 1 cut(s) 243
Ecl136II GAGCTC 1 cut(s) 1060
EclXI CGGCCG 1 cut(s) 539
Eco24I GRGCYC 2 cut(s) 202, 1062
Eco52I CGGCCG 1 cut(s) 539
Eco53kI GAGCTC 1 cut(s) 1060
Eco88I CYCGRG 1 cut(s) 223
EcoICRI GAGCTC 1 cut(s) 1060
EcoRII CCWGG 2 cut(s) 331, 736
EcoT22I ATGCAT 1 cut(s) 640
EcoT38I GRGCYC 2 cut(s) 202, 1062
EgeI GGCGCC 1 cut(s) 288
EheI GGCGCC 1 cut(s) 288
Esp3I CGTCTC 2 cut(s) 118, 972
FaeI CATG 4 cut(s) 103, 244, 379, 906
FalI AAGNNNNNCTT 2 cut(s) 572, 604
FatI CATG 4 cut(s) 99, 240, 375, 902
FauI CCCGC 2 cut(s) 210, 699
FauNDI CATATG 1 cut(s) 748
FbaI TGATCA 1 cut(s) 1047
Fnu4HI GCNGC 8 cut(s) 206, 214, 231, 234, 257, 290, 539, 704
FokI GGATG 2 cut(s) 322, 515
FriOI GRGCYC 2 cut(s) 202, 1062
Fsp4HI GCNGC 8 cut(s) 206, 214, 231, 234, 257, 290, 539, 704
FspI TGCGCA 2 cut(s) 85, 901
GlaI GCGC 5 cut(s) 85, 229, 288, 605, 901
GluI GCNGC 8 cut(s) 206, 214, 231, 234, 257, 290, 539, 704
HaeII RGCGCY 2 cut(s) 231, 290
HaeIII GGCC 1 cut(s) 541
HapII CCGG 1 cut(s) 319
HgaI GACGC 1 cut(s) 982
HhaI GCGC 5 cut(s) 86, 230, 289, 606, 902
Hin1I GRCGYC 2 cut(s) 287, 627
Hin1II CATG 4 cut(s) 103, 244, 379, 906
Hin6I GCGC 5 cut(s) 84, 228, 287, 604, 900
HinP1I GCGC 5 cut(s) 84, 228, 287, 604, 900
HinfI GANTC 6 cut(s) 221, 497, 617, 673, 718, 779
HpaII CCGG 1 cut(s) 319
HphI GGTGA 4 cut(s) 108, 310, 461, 533
Hpy166II GTNNAC 2 cut(s) 23, 574
Hpy188I TCNGA 8 cut(s) 220, 436, 496, 502, 670, 682, 865, 962
Hpy188III TCNNGA 4 cut(s) 34, 689, 722, 941
Hpy8I GTNNAC 2 cut(s) 23, 574
HpyAV CCTTC 3 cut(s) 545, 872, 876
HpyCH4III ACNGT 7 cut(s) 20, 42, 112, 281, 419, 449, 598
HpyCH4IV ACGT 2 cut(s) 627, 915
HpyCH4V TGCA 6 cut(s) 65, 70, 79, 638, 851, 919
HpyF10VI GCNNNNNNNGC 5 cut(s) 76, 899, 955, 964, 973
HpyF3I CTNAG 4 cut(s) 166, 354, 753, 864
HpySE526I ACGT 2 cut(s) 627, 915
Hsp92I GRCGYC 2 cut(s) 287, 627
Hsp92II CATG 4 cut(s) 103, 244, 379, 906
HspAI GCGC 5 cut(s) 84, 228, 287, 604, 900
KasI GGCGCC 1 cut(s) 286
KpnI GGTACC 1 cut(s) 319
Ksp22I TGATCA 1 cut(s) 1047
Kzo9I GATC 3 cut(s) 665, 685, 1047
LmnI GCTCC 4 cut(s) 213, 221, 387, 1057
Lsp1109I GCAGC 2 cut(s) 200, 715
LweI GCATC 2 cut(s) 357, 493
MaeII ACGT 2 cut(s) 627, 915
MaeIII GTNAC 2 cut(s) 34, 419
MalI GATC 3 cut(s) 667, 687, 1049
MbiI CCGCTC 1 cut(s) 208
MboI GATC 3 cut(s) 665, 685, 1047
MboII GAAGA 6 cut(s) 51, 423, 580, 661, 695, 920
MflI RGATCY 1 cut(s) 685
MhlI GDGCHC 4 cut(s) 33, 202, 969, 1062
MluCI AATT 7 cut(s) 7, 53, 72, 130, 171, 404, 424
Mly113I GGCGCC 1 cut(s) 287
MlyI GAGTC 3 cut(s) 215, 682, 788
MmeI TCCRAC 1 cut(s) 243
Mph1103I ATGCAT 1 cut(s) 640
MseI TTAA 3 cut(s) 6, 699, 998
MspA1I CMGCKG 2 cut(s) 213, 706
MspI CCGG 1 cut(s) 319
MspR9I CCNGG 2 cut(s) 333, 738
Mva1269I GAATGC 1 cut(s) 166
MvaI CCWGG 2 cut(s) 333, 738
MvnI CGCG 1 cut(s) 976
MwoI GCNNNNNNNGC 5 cut(s) 76, 899, 955, 964, 973
NarI GGCGCC 1 cut(s) 287
NdeI CATATG 1 cut(s) 748
NdeII GATC 3 cut(s) 665, 685, 1047
NlaIII CATG 4 cut(s) 103, 244, 379, 906
NlaIV GGNNCC 2 cut(s) 288, 317
NmeAIII GCCGAG 1 cut(s) 567
NmuCI GTSAC 1 cut(s) 34
NsbI TGCGCA 2 cut(s) 85, 901
NsiI ATGCAT 1 cut(s) 640
PaeR7I CTCGAG 1 cut(s) 223
PasI CCCWGGG 1 cut(s) 332
PcsI WCGNNNNNNNCGW 2 cut(s) 132, 597
PctI GAATGC 1 cut(s) 166
PfeI GAWTC 3 cut(s) 497, 617, 718
PflFI GACNNNGTC 1 cut(s) 596
PfoI TCCNGGA 1 cut(s) 736
PkrI GCNGC 8 cut(s) 207, 215, 232, 235, 258, 291, 540, 705
PleI GAGTC 3 cut(s) 215, 681, 787
PluTI GGCGCC 1 cut(s) 290
PpsI GAGTC 3 cut(s) 215, 681, 787
PshBI ATTAAT 1 cut(s) 6
Psp124BI GAGCTC 1 cut(s) 1062
Psp6I CCWGG 2 cut(s) 331, 736
PspGI CCWGG 2 cut(s) 331, 736
PspN4I GGNNCC 2 cut(s) 288, 317
PspXI VCTCGAGB 1 cut(s) 223
PsuI RGATCY 1 cut(s) 685
PsyI GACNNNGTC 1 cut(s) 596
RsaI GTAC 4 cut(s) 283, 317, 329, 566
RsaNI GTAC 4 cut(s) 282, 316, 328, 565
SacI GAGCTC 1 cut(s) 1062
SaqAI TTAA 3 cut(s) 6, 699, 998
SatI GCNGC 8 cut(s) 206, 214, 231, 234, 257, 290, 539, 704
Sau3AI GATC 3 cut(s) 665, 685, 1047
ScaI AGTACT 1 cut(s) 566
SchI GAGTC 3 cut(s) 215, 682, 788
ScrFI CCNGG 2 cut(s) 333, 738
SduI GDGCHC 4 cut(s) 33, 202, 969, 1062
SfaNI GCATC 2 cut(s) 357, 493
SfoI GGCGCC 1 cut(s) 288
Sfr274I CTCGAG 1 cut(s) 223
SlaI CTCGAG 1 cut(s) 223
SmlI CTYRAG 2 cut(s) 223, 1061
SmoI CTYRAG 2 cut(s) 223, 1061
Sse9I AATT 7 cut(s) 7, 53, 72, 130, 171, 404, 424
SspDI GGCGCC 1 cut(s) 286
SstI GAGCTC 1 cut(s) 1062
StyD4I CCNGG 2 cut(s) 331, 736
TaaI ACNGT 7 cut(s) 20, 42, 112, 281, 419, 449, 598
TaiI ACGT 2 cut(s) 630, 918
TasI AATT 7 cut(s) 7, 53, 72, 130, 171, 404, 424
TatI WGTACW 1 cut(s) 564
TauI GCSGC 6 cut(s) 208, 233, 236, 259, 292, 541
TfiI GAWTC 3 cut(s) 497, 617, 718
Tru1I TTAA 3 cut(s) 6, 699, 998
Tru9I TTAA 3 cut(s) 6, 699, 998
TscAI CASTG 3 cut(s) 45, 358, 974
TseFI GTSAC 1 cut(s) 34
TseI GCWGC 2 cut(s) 213, 703
Tsp45I GTSAC 1 cut(s) 34
TspDTI ATGAA 4 cut(s) 88, 392, 630, 735
TspGWI ACGGA 1 cut(s) 542
TspRI CASTG 3 cut(s) 45, 358, 974
Tth111I GACNNNGTC 1 cut(s) 596
VspI ATTAAT 1 cut(s) 6
XhoI CTCGAG 1 cut(s) 223
ZraI GACGTC 1 cut(s) 628
ZrmI AGTACT 1 cut(s) 566
Zsp2I ATGCAT 1 cut(s) 640
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.