RLG00000010727
NAC Family

inactive heme oxygenase 2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0009205
Physical Location & Seq
Forward (+)
3953483 .. 3954912
1430 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000010727

Sequence Viewer

Length: 963 bp
ATGCAATACGCAACGACGTCGTTAATGGACAAAGCGGTGTCCCCATCAATTCTTTACACCCTTTCACCCAAGCCCTCCCCAATTTCAAGCAGAAAGCTCCCCTCCCTGTTTCTCTGCTGCTCCGATTCGAGCACCACCGCCGCCTCCACCTCCACCTCTTGGTCTCCGCCTCCGATTCGGGCCACCACCGGCACGGCGCCGCCGGTCGTGAGGCGGAGGACGAGGTACAGGAAGCAGTACCCGGGAGAGAGCAAAGGCATCACCGAAGAGATGAGGTTCGTGGCCATGAGGCTCCGTAACAGAAACGGCAAGAAACTGGACCCCCGATCTGACGGTGATGATGATACTCAGAGTGAAAGTGACGATAATGCCCCTGACGACAGTGATGCTTCGGAATCTGAGGGCGAGGGCGAGGGCGAGAAGGAGACTTGGCAGCCTAGTGTGGAGGGGTTTCTCAAGTACTTGGTGGACAGTAAGCTCGTGTTTGACACCGTGGAGCGCATTGTTGATGATTCAAACGACGTCGCTTACGCGTATTTCAGGAATACGGGATTGGAAAGATCAGAAGCGCTTGCGAAAGATTTGCAATGGTTTGGTGAACAGGGCAATGTGATTCCGGAACCCAGCAATCCAGGAGTTTCTTATGCCAAGTATCTTAAACAACTTGCAGAAACTTGTGCGCCATTGTTCCTCTGCCACTTTTATAATATATATTTTTCACATATATCTGGTGGGCAGGTCATAGCAAGACAGGTATCTGAGAGGCTACTTGAAGGAAGGGAGTTGGAGTTTTACACATGGGAAGGCGATGTGCCTGAGTTAATGAGAGGGGTTCGTGAGAAGCTCAACATGCTTGGAGAGCACTGGAGTCGAGATGACAAGAACAAATGCTTAAGAGAAACAACCAAGTCATTCCGATCTCTGGGGCAGATAATTCGCCTGATCATCTTAGAAACCAAGTGA

Protein Analysis

321

Amino Acids

36.13

Weight (kDa)

5.4

Isoelectric Point (pI)

49.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Heme_oxygenase PF01126 149 - 311 2.8e-11 Heme oxygenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0009205)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G26550 AT2G26550 AT2G26550 AT2G26550
fragaria_vesca FvH4_2g34841 FvH4_2g34841 FvH4_2g34841 FvH4_2g34841 FvH4_2g34841
malus_domestica MD15G1086700.v1.1
prunus_persica Prupe.1G440300_v2.0.a1
pyrus_communis pycom15g08160
rosa_chinensis RchiOBHm_Chr6g0307211
rosa_laevigata RLG00000010727
rosa_multiflora Rmu_sc0041325.1_g000001
rosa_roxburghii Rroxscaffold_7G00161080
rosa_rugosa Rorug06G0357700
rosa_samantha Rh6AG468300 Rh6BG432200 Rh6CG483500 Rh6DG470100
rosa_wichuraiana Rw6G040800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 705
AatII GACGTC 2 cut(s) 20, 525
Acc36I ACCTGC 1 cut(s) 727
AccB1I GGYRCC 1 cut(s) 196
AccB7I CCANNNNNTGG 1 cut(s) 159
AccII CGCG 1 cut(s) 533
AccIII TCCGGA 1 cut(s) 616
AciI CCGC 6 cut(s) 35, 138, 141, 167, 200, 214
AcoI YGGCCR 1 cut(s) 282
AcyI GRCGYC 3 cut(s) 17, 197, 522
AfaI GTAC 3 cut(s) 227, 239, 461
AfeI AGCGCT 1 cut(s) 570
AfiI CCNNNNNNNGG 2 cut(s) 159, 922
AflII CTTAAG 1 cut(s) 892
AflIII ACRYGT 1 cut(s) 531
AgsI TTSAA 3 cut(s) 87, 516, 773
AjnI CCWGG 1 cut(s) 631
AjuI GAANNNNNNNTTGG 2 cut(s) 536, 568
AluBI AGCT 3 cut(s) 97, 478, 844
AluI AGCT 3 cut(s) 97, 478, 844
Alw21I GWGCWC 2 cut(s) 134, 864
Alw26I GTCTC 2 cut(s) 168, 419
Ama87I CYCGRG 1 cut(s) 241
Aor13HI TCCGGA 1 cut(s) 616
Aor51HI AGCGCT 1 cut(s) 570
AoxI GGCC 2 cut(s) 180, 282
ApeKI GCWGC 2 cut(s) 117, 433
AspLEI GCGC 4 cut(s) 199, 501, 571, 682
AspS9I GGNCC 2 cut(s) 180, 319
AsuC2I CCSGG 2 cut(s) 242, 243
AsuHPI GGTGA 4 cut(s) 57, 253, 347, 608
AvaI CYCGRG 1 cut(s) 241
AvaII GGWCC 1 cut(s) 319
BalI TGGCCA 1 cut(s) 284
BanI GGYRCC 1 cut(s) 196
BauI CACGAG 1 cut(s) 479
Bbv12I GWGCWC 2 cut(s) 134, 864
BbvI GCAGC 2 cut(s) 104, 445
BccI CCATC 1 cut(s) 52
BceAI ACGGC 2 cut(s) 210, 322
BcgI CGANNNNNNTGC 9 cut(s) 34, 368, 402, 565, 599, 851, 885, 917, 951
BciT130I CCWGG 1 cut(s) 633
BclI TGATCA 1 cut(s) 942
BcnI CCSGG 2 cut(s) 242, 243
BcoDI GTCTC 2 cut(s) 168, 419
BfaI CTAG 1 cut(s) 438
BfoI RGCGCY 2 cut(s) 200, 572
BfrI CTTAAG 1 cut(s) 892
BfuAI ACCTGC 1 cut(s) 727
BisI GCNGC 4 cut(s) 118, 141, 200, 434
BlsI GCNGC 4 cut(s) 119, 142, 201, 435
BmcAI AGTACT 1 cut(s) 461
Bme1390I CCNGG 3 cut(s) 242, 243, 633
Bme18I GGWCC 1 cut(s) 319
BmeT110I CYCGRG 1 cut(s) 241
BmgT120I GGNCC 2 cut(s) 180, 319
BmiI GGNNCC 4 cut(s) 198, 293, 321, 621
BmrFI CCNGG 3 cut(s) 242, 243, 633
BmsI GCATC 2 cut(s) 267, 376
BpmI CTGGAG 1 cut(s) 886
BpuEI CTTGAG 1 cut(s) 440
BpuMI CCSGG 2 cut(s) 242, 243
BsaHI GRCGYC 3 cut(s) 17, 197, 522
BsaI GGTCTC 1 cut(s) 168
BsaJI CCNNGG 2 cut(s) 241, 492
BsaWI WCCGGW 1 cut(s) 616
BsaXI ACNNNNNCTCC 2 cut(s) 773, 803
Bsc4I CCNNNNNNNGG 2 cut(s) 159, 922
Bse118I RCCGGY 2 cut(s) 188, 202
Bse1I ACTGG 2 cut(s) 321, 869
Bse3DI GCAATG 2 cut(s) 593, 613
BseAI TCCGGA 1 cut(s) 616
BseBI CCWGG 1 cut(s) 633
BseDI CCNNGG 2 cut(s) 241, 492
BseLI CCNNNNNNNGG 2 cut(s) 159, 922
BseMI GCAATG 2 cut(s) 593, 613
BseMII CTCAG 4 cut(s) 362, 390, 750, 807
BseNI ACTGG 2 cut(s) 321, 869
BseXI GCAGC 2 cut(s) 104, 445
BseYI CCCAGC 1 cut(s) 623
Bsh1236I CGCG 1 cut(s) 533
Bsh1285I CGRYCG 1 cut(s) 207
BshFI GGCC 2 cut(s) 182, 284
BshNI GGYRCC 1 cut(s) 196
BsiEI CGRYCG 1 cut(s) 207
BsiHKAI GWGCWC 2 cut(s) 134, 864
BsiHKCI CYCGRG 1 cut(s) 241
BsiSI CCGG 4 cut(s) 189, 203, 242, 617
BslFI GGGAC 1 cut(s) 25
BslI CCNNNNNNNGG 2 cut(s) 159, 922
BsmAI GTCTC 2 cut(s) 168, 419
BsmFI GGGAC 1 cut(s) 25
BsnI GGCC 2 cut(s) 182, 284
Bso31I GGTCTC 1 cut(s) 168
BsoBI CYCGRG 1 cut(s) 241
Bsp1286I GDGCHC 2 cut(s) 134, 864
Bsp13I TCCGGA 1 cut(s) 616
Bsp143I GATC 4 cut(s) 326, 560, 917, 942
BspACI CCGC 6 cut(s) 35, 138, 141, 167, 200, 214
BspANI GGCC 2 cut(s) 182, 284
BspCNI CTCAG 4 cut(s) 361, 391, 751, 808
BspEI TCCGGA 1 cut(s) 616
BspFNI CGCG 1 cut(s) 533
BspLI GGNNCC 4 cut(s) 198, 293, 321, 621
BspMI ACCTGC 1 cut(s) 727
BspT107I GGYRCC 1 cut(s) 196
BspTI CTTAAG 1 cut(s) 892
BspTNI GGTCTC 1 cut(s) 168
BsrDI GCAATG 2 cut(s) 593, 613
BsrFI RCCGGY 2 cut(s) 188, 202
BsrI ACTGG 2 cut(s) 321, 869
BssAI RCCGGY 2 cut(s) 188, 202
BssECI CCNNGG 2 cut(s) 241, 492
BssMI GATC 4 cut(s) 326, 560, 917, 942
BssNI GRCGYC 3 cut(s) 17, 197, 522
BssSI CACGAG 1 cut(s) 479
Bst2BI CACGAG 1 cut(s) 479
Bst2UI CCWGG 1 cut(s) 633
Bst4CI ACNGT 4 cut(s) 335, 383, 473, 493
Bst6I CTCTTC 1 cut(s) 261
BstACI GRCGYC 3 cut(s) 17, 197, 522
BstAFI CTTAAG 1 cut(s) 892
BstC8I GCNNGC 1 cut(s) 573
BstDEI CTNAG 5 cut(s) 348, 399, 759, 816, 949
BstDSI CCRYGG 1 cut(s) 492
BstFNI CGCG 1 cut(s) 533
BstH2I RGCGCY 2 cut(s) 200, 572
BstHHI GCGC 4 cut(s) 199, 501, 571, 682
BstKTI GATC 4 cut(s) 329, 563, 920, 945
BstMAI GTCTC 2 cut(s) 168, 419
BstMBI GATC 4 cut(s) 326, 560, 917, 942
BstMCI CGRYCG 1 cut(s) 207
BstMWI GCNNNNNNNGC 2 cut(s) 850, 859
BstNI CCWGG 1 cut(s) 633
BstNSI RCATGY 1 cut(s) 853
BstSCI CCNGG 3 cut(s) 240, 241, 631
BstUI CGCG 1 cut(s) 533
BstV1I GCAGC 2 cut(s) 104, 445
BsuRI GGCC 2 cut(s) 182, 284
BtgI CCRYGG 1 cut(s) 492
BtgZI GCGATG 1 cut(s) 822
BtsIMutI CAGTG 2 cut(s) 388, 862
BveI ACCTGC 1 cut(s) 727
Cac8I GCNNGC 1 cut(s) 573
CfoI GCGC 4 cut(s) 199, 501, 571, 682
Cfr10I RCCGGY 2 cut(s) 188, 202
Cfr13I GGNCC 2 cut(s) 180, 319
Cfr9I CCCGGG 1 cut(s) 241
Csp6I GTAC 3 cut(s) 226, 238, 460
CviAII CATG 3 cut(s) 286, 798, 850
CviJI RGCY 9 cut(s) 73, 97, 182, 284, 292, 436, 478, 766, 844
CviKI_1 RGCY 9 cut(s) 73, 97, 182, 284, 292, 436, 478, 766, 844
CviQI GTAC 3 cut(s) 226, 238, 460
DdeI CTNAG 5 cut(s) 348, 399, 759, 816, 949
DinI GGCGCC 1 cut(s) 198
DpnI GATC 4 cut(s) 328, 562, 919, 944
DpnII GATC 4 cut(s) 326, 560, 917, 942
EaeI YGGCCR 1 cut(s) 282
Eam1104I CTCTTC 1 cut(s) 261
EarI CTCTTC 1 cut(s) 261
EciI GGCGGA 2 cut(s) 156, 229
Eco31I GGTCTC 1 cut(s) 168
Eco47I GGWCC 1 cut(s) 319
Eco47III AGCGCT 1 cut(s) 570
Eco88I CYCGRG 1 cut(s) 241
EcoRII CCWGG 1 cut(s) 631
EgeI GGCGCC 1 cut(s) 198
EheI GGCGCC 1 cut(s) 198
FaeI CATG 3 cut(s) 289, 801, 853
FaqI GGGAC 1 cut(s) 25
FatI CATG 3 cut(s) 285, 797, 849
FbaI TGATCA 1 cut(s) 942
Fnu4HI GCNGC 4 cut(s) 118, 141, 200, 434
Fsp4HI GCNGC 4 cut(s) 118, 141, 200, 434
FspBI CTAG 1 cut(s) 438
GlaI GCGC 4 cut(s) 198, 500, 570, 681
GluI GCNGC 4 cut(s) 118, 141, 200, 434
GsaI CCCAGC 1 cut(s) 627
GsuI CTGGAG 1 cut(s) 886
HaeII RGCGCY 2 cut(s) 200, 572
HaeIII GGCC 2 cut(s) 182, 284
HapII CCGG 4 cut(s) 189, 203, 242, 617
HhaI GCGC 4 cut(s) 199, 501, 571, 682
Hin1I GRCGYC 3 cut(s) 17, 197, 522
Hin1II CATG 3 cut(s) 289, 801, 853
Hin6I GCGC 4 cut(s) 197, 499, 569, 680
HinP1I GCGC 4 cut(s) 197, 499, 569, 680
HinfI GANTC 6 cut(s) 125, 175, 395, 512, 613, 868
HpaII CCGG 4 cut(s) 189, 203, 242, 617
HphI GGTGA 4 cut(s) 57, 253, 347, 608
Hpy166II GTNNAC 2 cut(s) 469, 599
Hpy188I TCNGA 9 cut(s) 124, 174, 331, 351, 394, 400, 565, 760, 917
Hpy188III TCNNGA 5 cut(s) 208, 541, 617, 836, 872
Hpy8I GTNNAC 2 cut(s) 469, 599
Hpy99I CGWCG 4 cut(s) 19, 22, 524, 527
HpyAV CCTTC 4 cut(s) 415, 767, 771, 797
HpyCH4III ACNGT 4 cut(s) 335, 383, 473, 493
HpyCH4IV ACGT 2 cut(s) 17, 522
HpyCH4V TGCA 3 cut(s) 4, 586, 668
HpyF10VI GCNNNNNNNGC 2 cut(s) 850, 859
HpyF3I CTNAG 5 cut(s) 348, 399, 759, 816, 949
HpySE526I ACGT 2 cut(s) 17, 522
Hsp92I GRCGYC 3 cut(s) 17, 197, 522
Hsp92II CATG 3 cut(s) 289, 801, 853
HspAI GCGC 4 cut(s) 197, 499, 569, 680
KasI GGCGCC 1 cut(s) 196
Kpn2I TCCGGA 1 cut(s) 616
Ksp22I TGATCA 1 cut(s) 942
Kzo9I GATC 4 cut(s) 326, 560, 917, 942
LmnI GCTCC 4 cut(s) 102, 125, 297, 496
Lsp1109I GCAGC 2 cut(s) 104, 445
LweI GCATC 2 cut(s) 267, 376
MaeI CTAG 1 cut(s) 438
MaeII ACGT 2 cut(s) 17, 522
MaeIII GTNAC 2 cut(s) 296, 359
MalI GATC 4 cut(s) 328, 562, 919, 944
MboI GATC 4 cut(s) 326, 560, 917, 942
MboII GAAGA 1 cut(s) 278
MhlI GDGCHC 2 cut(s) 134, 864
MlsI TGGCCA 1 cut(s) 284
MluCI AATT 3 cut(s) 48, 81, 933
MluI ACGCGT 1 cut(s) 531
MluNI TGGCCA 1 cut(s) 284
Mly113I GGCGCC 1 cut(s) 197
MlyI GAGTC 1 cut(s) 877
MmeI TCCRAC 1 cut(s) 765
Mox20I TGGCCA 1 cut(s) 284
MroI TCCGGA 1 cut(s) 616
MscI TGGCCA 1 cut(s) 284
MseI TTAA 4 cut(s) 23, 657, 821, 893
Msp20I TGGCCA 1 cut(s) 284
MspCI CTTAAG 1 cut(s) 892
MspI CCGG 4 cut(s) 189, 203, 242, 617
MspR9I CCNGG 3 cut(s) 242, 243, 633
MvaI CCWGG 1 cut(s) 633
MvnI CGCG 1 cut(s) 533
MwoI GCNNNNNNNGC 2 cut(s) 850, 859
NarI GGCGCC 1 cut(s) 197
NciI CCSGG 2 cut(s) 242, 243
NdeII GATC 4 cut(s) 326, 560, 917, 942
NlaIII CATG 3 cut(s) 289, 801, 853
NlaIV GGNNCC 4 cut(s) 198, 293, 321, 621
NmuCI GTSAC 1 cut(s) 359
NspI RCATGY 1 cut(s) 853
PfeI GAWTC 5 cut(s) 125, 175, 395, 512, 613
PflMI CCANNNNNTGG 1 cut(s) 159
PfoI TCCNGGA 1 cut(s) 631
PkrI GCNGC 4 cut(s) 119, 142, 201, 435
PleI GAGTC 1 cut(s) 876
PluTI GGCGCC 1 cut(s) 200
PpsI GAGTC 1 cut(s) 876
PsiI TTATAA 1 cut(s) 705
Psp6I CCWGG 1 cut(s) 631
PspFI CCCAGC 1 cut(s) 623
PspGI CCWGG 1 cut(s) 631
PspN4I GGNNCC 4 cut(s) 198, 293, 321, 621
PspPI GGNCC 2 cut(s) 180, 319
RsaI GTAC 3 cut(s) 227, 239, 461
RsaNI GTAC 3 cut(s) 226, 238, 460
SaqAI TTAA 4 cut(s) 23, 657, 821, 893
SatI GCNGC 4 cut(s) 118, 141, 200, 434
Sau3AI GATC 4 cut(s) 326, 560, 917, 942
Sau96I GGNCC 2 cut(s) 180, 319
ScaI AGTACT 1 cut(s) 461
SchI GAGTC 1 cut(s) 877
ScrFI CCNGG 3 cut(s) 242, 243, 633
SduI GDGCHC 2 cut(s) 134, 864
SfaNI GCATC 2 cut(s) 267, 376
SfoI GGCGCC 1 cut(s) 198
SinI GGWCC 1 cut(s) 319
SmaI CCCGGG 1 cut(s) 243
SmlI CTYRAG 2 cut(s) 455, 892
SmoI CTYRAG 2 cut(s) 455, 892
Sse9I AATT 3 cut(s) 48, 81, 933
SsiI CCGC 6 cut(s) 35, 138, 141, 167, 200, 214
SspDI GGCGCC 1 cut(s) 196
SspMI CTAG 1 cut(s) 438
StyD4I CCNGG 3 cut(s) 240, 241, 631
TaaI ACNGT 4 cut(s) 335, 383, 473, 493
TaiI ACGT 2 cut(s) 20, 525
TaqI TCGA 2 cut(s) 128, 871
TasI AATT 3 cut(s) 48, 81, 933
TatI WGTACW 1 cut(s) 459
TauI GCSGC 2 cut(s) 143, 202
TfiI GAWTC 5 cut(s) 125, 175, 395, 512, 613
Tru1I TTAA 4 cut(s) 23, 657, 821, 893
Tru9I TTAA 4 cut(s) 23, 657, 821, 893
TscAI CASTG 2 cut(s) 388, 869
TseFI GTSAC 1 cut(s) 359
TseI GCWGC 2 cut(s) 117, 433
Tsp45I GTSAC 1 cut(s) 359
TspGWI ACGGA 1 cut(s) 284
TspMI CCCGGG 1 cut(s) 241
TspRI CASTG 2 cut(s) 388, 869
Van91I CCANNNNNTGG 1 cut(s) 159
Vha464I CTTAAG 1 cut(s) 892
VpaK11BI GGWCC 1 cut(s) 319
XceI RCATGY 1 cut(s) 853
XmaI CCCGGG 1 cut(s) 241
XspI CTAG 1 cut(s) 438
ZraI GACGTC 2 cut(s) 18, 523
ZrmI AGTACT 1 cut(s) 461
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.