Rroxscaffold_7G00161080
NAC Family

inactive heme oxygenase 2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0009205
Physical Location & Seq
Forward (+)
3924785 .. 3926996
2212 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00161080.1

Sequence Viewer

Length: 966 bp
ATGCAATACGCAACGACGTCGTTAATGGACAAAGCGGTGTCCCCATCAATTCTTTACACCCTTTCACCCAAGCCCTCCCCAATTTCAACCAGAAAGCTTTCCTCCCTGTTTCTCTGCTGCTCCGATTCGAGCACCACCGCCGCCTCCGCCTCCACCTCCACCTGTTGGTCTCCGCCTCCGATTCCGGCCACCACCGGCACGGCGCCGCCGGTCGTGAGGCGGAGGACGAGGTACAGGAAGCAGTACCCGGGAGAGAGCAAAGGCATCACCGAAGAGATGAGGTTCGTCGCCATGAGGCTCCGTAACAGAAACGGCAAGAAACTCGACTCCCGATCTGACGGTGATGATACTGAGAGTGAAAGTGACGATAATGCCCCCGACGACAGTGATGCTTCGGAATCTGACGGCGACGGCGGCGGCGAGAAGGAGACTTGGCAGCCTAGTGTGGAGGGGTTTCTCAAGTACTTGGTGGACAGTAAGCTCGTGTTTGACACCGTGGAGCGCATTGTTGATGAATCAAACGACGTCGCTTACGCGTATTTCAGGAATACGGGATTGGAAAGATCAGAAGCGCTTGCGAAAGATTTGCAATGGTTTGGTGAAGAGGGCAATGTGATTCCGGAACCCAGCAATCCAGGAGTTTCTTATGCCAAGAATCTTAAACAACTTGCAGAAACTTGTGCGCCATTGTTCCTCTGCCACTTTTATAATATATATTTTTCACATATATCTGGTGGGCAGCTCATAGCAAGACAGGTATCTGAGAGGCTACTTGAAGGAAGGGAGTTGGAGTTTTACACATGGGAAGGCGATGTGCCAGAGCTAATGAGAGGGGTTCGTGAGAAGCTCAACAAGCTTGGAGAGCACTGGAGTCGAGATGACAAGAACAAATGCTTGAGAGAAACAACCAAGTCATTCCGATCTCTGGGCCAGATAATTCGCCTGATCATCTTAGAAACCAAGTGA

Protein Analysis

321

Amino Acids

36.0

Weight (kDa)

5.4

Isoelectric Point (pI)

52.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Heme_oxygenase PF01126 150 - 313 4.8e-12 Heme oxygenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0009205)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G26550 AT2G26550 AT2G26550 AT2G26550
fragaria_vesca FvH4_2g34841 FvH4_2g34841 FvH4_2g34841 FvH4_2g34841 FvH4_2g34841
malus_domestica MD15G1086700.v1.1
prunus_persica Prupe.1G440300_v2.0.a1
pyrus_communis pycom15g08160
rosa_chinensis RchiOBHm_Chr6g0307211
rosa_laevigata RLG00000010727
rosa_multiflora Rmu_sc0041325.1_g000001
rosa_roxburghii Rroxscaffold_7G00161080
rosa_rugosa Rorug06G0357700
rosa_samantha Rh6AG468300 Rh6BG432200 Rh6CG483500 Rh6DG470100
rosa_wichuraiana Rw6G040800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 708
AatII GACGTC 2 cut(s) 20, 528
AccB1I GGYRCC 1 cut(s) 202
AccB7I CCANNNNNTGG 1 cut(s) 165
AccII CGCG 1 cut(s) 536
AccIII TCCGGA 1 cut(s) 619
AciI CCGC 9 cut(s) 35, 138, 141, 147, 173, 206, 220, 414, 417
AcoI YGGCCR 1 cut(s) 186
AcyI GRCGYC 3 cut(s) 17, 203, 525
AfaI GTAC 3 cut(s) 233, 245, 464
AfeI AGCGCT 1 cut(s) 573
AfiI CCNNNNNNNGG 2 cut(s) 165, 925
AflIII ACRYGT 1 cut(s) 534
AgsI TTSAA 2 cut(s) 87, 776
AjnI CCWGG 1 cut(s) 634
AjuI GAANNNNNNNTTGG 2 cut(s) 539, 571
AluBI AGCT 6 cut(s) 97, 481, 742, 823, 847, 856
AluI AGCT 6 cut(s) 97, 481, 742, 823, 847, 856
Alw21I GWGCWC 2 cut(s) 134, 867
Alw26I GTCTC 2 cut(s) 174, 422
Ama87I CYCGRG 1 cut(s) 247
Aor13HI TCCGGA 1 cut(s) 619
Aor51HI AGCGCT 1 cut(s) 573
AoxI GGCC 2 cut(s) 186, 928
ApeKI GCWGC 3 cut(s) 117, 436, 739
Asp700I GAANNNNTTC 1 cut(s) 97
AspLEI GCGC 4 cut(s) 205, 504, 574, 685
AspS9I GGNCC 1 cut(s) 928
AsuC2I CCSGG 2 cut(s) 248, 249
AsuHPI GGTGA 4 cut(s) 57, 259, 353, 611
AvaI CYCGRG 1 cut(s) 247
BanI GGYRCC 1 cut(s) 202
BauI CACGAG 1 cut(s) 482
Bbv12I GWGCWC 2 cut(s) 134, 867
BbvI GCAGC 3 cut(s) 104, 448, 751
BccI CCATC 1 cut(s) 52
BceAI ACGGC 4 cut(s) 216, 328, 421, 427
BcgI CGANNNNNNTGC 9 cut(s) 34, 304, 338, 371, 405, 568, 602, 854, 888
BciT130I CCWGG 1 cut(s) 636
BclI TGATCA 1 cut(s) 945
BcnI CCSGG 2 cut(s) 248, 249
BcoDI GTCTC 2 cut(s) 174, 422
BfaI CTAG 1 cut(s) 441
BfoI RGCGCY 2 cut(s) 206, 575
BisI GCNGC 7 cut(s) 118, 141, 206, 415, 418, 437, 740
BlsI GCNGC 7 cut(s) 119, 142, 207, 416, 419, 438, 741
BmcAI AGTACT 1 cut(s) 464
Bme1390I CCNGG 3 cut(s) 248, 249, 636
BmeT110I CYCGRG 1 cut(s) 247
BmgT120I GGNCC 1 cut(s) 928
BmiI GGNNCC 3 cut(s) 204, 299, 624
BmrFI CCNGG 3 cut(s) 248, 249, 636
BmsI GCATC 2 cut(s) 273, 379
BpmI CTGGAG 1 cut(s) 889
BpuEI CTTGAG 2 cut(s) 443, 916
BpuMI CCSGG 2 cut(s) 248, 249
BsaHI GRCGYC 3 cut(s) 17, 203, 525
BsaI GGTCTC 1 cut(s) 174
BsaJI CCNNGG 2 cut(s) 247, 495
BsaWI WCCGGW 1 cut(s) 619
BsaXI ACNNNNNCTCC 2 cut(s) 776, 806
Bsc4I CCNNNNNNNGG 2 cut(s) 165, 925
Bse118I RCCGGY 2 cut(s) 194, 208
Bse1I ACTGG 1 cut(s) 872
Bse3DI GCAATG 2 cut(s) 596, 616
BseAI TCCGGA 1 cut(s) 619
BseBI CCWGG 1 cut(s) 636
BseDI CCNNGG 2 cut(s) 247, 495
BseLI CCNNNNNNNGG 2 cut(s) 165, 925
BseMI GCAATG 2 cut(s) 596, 616
BseMII CTCAG 2 cut(s) 342, 753
BseNI ACTGG 1 cut(s) 872
BseXI GCAGC 3 cut(s) 104, 448, 751
BseYI CCCAGC 1 cut(s) 626
Bsh1236I CGCG 1 cut(s) 536
Bsh1285I CGRYCG 1 cut(s) 213
BshFI GGCC 2 cut(s) 188, 930
BshNI GGYRCC 1 cut(s) 202
BsiEI CGRYCG 1 cut(s) 213
BsiHKAI GWGCWC 2 cut(s) 134, 867
BsiHKCI CYCGRG 1 cut(s) 247
BsiSI CCGG 5 cut(s) 185, 195, 209, 248, 620
BslFI GGGAC 1 cut(s) 25
BslI CCNNNNNNNGG 2 cut(s) 165, 925
BsmAI GTCTC 2 cut(s) 174, 422
BsmFI GGGAC 1 cut(s) 25
BsnI GGCC 2 cut(s) 188, 930
Bso31I GGTCTC 1 cut(s) 174
BsoBI CYCGRG 1 cut(s) 247
Bsp1286I GDGCHC 2 cut(s) 134, 867
Bsp13I TCCGGA 1 cut(s) 619
Bsp143I GATC 4 cut(s) 332, 563, 920, 945
BspACI CCGC 9 cut(s) 35, 138, 141, 147, 173, 206, 220, 414, 417
BspANI GGCC 2 cut(s) 188, 930
BspCNI CTCAG 2 cut(s) 343, 754
BspEI TCCGGA 1 cut(s) 619
BspFNI CGCG 1 cut(s) 536
BspLI GGNNCC 3 cut(s) 204, 299, 624
BspT107I GGYRCC 1 cut(s) 202
BspTNI GGTCTC 1 cut(s) 174
BsrDI GCAATG 2 cut(s) 596, 616
BsrFI RCCGGY 2 cut(s) 194, 208
BsrI ACTGG 1 cut(s) 872
BssAI RCCGGY 2 cut(s) 194, 208
BssECI CCNNGG 2 cut(s) 247, 495
BssMI GATC 4 cut(s) 332, 563, 920, 945
BssNI GRCGYC 3 cut(s) 17, 203, 525
BssSI CACGAG 1 cut(s) 482
Bst2BI CACGAG 1 cut(s) 482
Bst2UI CCWGG 1 cut(s) 636
Bst4CI ACNGT 4 cut(s) 341, 386, 476, 496
Bst6I CTCTTC 2 cut(s) 267, 597
BstACI GRCGYC 3 cut(s) 17, 203, 525
BstC8I GCNNGC 1 cut(s) 576
BstDEI CTNAG 3 cut(s) 351, 762, 952
BstDSI CCRYGG 1 cut(s) 495
BstFNI CGCG 1 cut(s) 536
BstH2I RGCGCY 2 cut(s) 206, 575
BstHHI GCGC 4 cut(s) 205, 504, 574, 685
BstKTI GATC 4 cut(s) 335, 566, 923, 948
BstMAI GTCTC 2 cut(s) 174, 422
BstMBI GATC 4 cut(s) 332, 563, 920, 945
BstMCI CGRYCG 1 cut(s) 213
BstMWI GCNNNNNNNGC 4 cut(s) 146, 414, 853, 862
BstNI CCWGG 1 cut(s) 636
BstSCI CCNGG 3 cut(s) 246, 247, 634
BstUI CGCG 1 cut(s) 536
BstV1I GCAGC 3 cut(s) 104, 448, 751
BsuRI GGCC 2 cut(s) 188, 930
BtgI CCRYGG 1 cut(s) 495
BtgZI GCGATG 1 cut(s) 825
BtsIMutI CAGTG 2 cut(s) 391, 865
Cac8I GCNNGC 1 cut(s) 576
CfoI GCGC 4 cut(s) 205, 504, 574, 685
Cfr10I RCCGGY 2 cut(s) 194, 208
Cfr13I GGNCC 1 cut(s) 928
Cfr9I CCCGGG 1 cut(s) 247
Csp6I GTAC 3 cut(s) 232, 244, 463
CviAII CATG 2 cut(s) 292, 801
CviQI GTAC 3 cut(s) 232, 244, 463
DdeI CTNAG 3 cut(s) 351, 762, 952
DinI GGCGCC 1 cut(s) 204
DpnI GATC 4 cut(s) 334, 565, 922, 947
DpnII GATC 4 cut(s) 332, 563, 920, 945
EaeI YGGCCR 1 cut(s) 186
Eam1104I CTCTTC 2 cut(s) 267, 597
EarI CTCTTC 2 cut(s) 267, 597
EciI GGCGGA 3 cut(s) 136, 162, 235
Eco31I GGTCTC 1 cut(s) 174
Eco47III AGCGCT 1 cut(s) 573
Eco88I CYCGRG 1 cut(s) 247
EcoRII CCWGG 1 cut(s) 634
EgeI GGCGCC 1 cut(s) 204
EheI GGCGCC 1 cut(s) 204
FaeI CATG 2 cut(s) 295, 804
FaiI YATR 9 cut(s) 293, 648, 708, 713, 715, 726, 728, 746, 802
FaqI GGGAC 1 cut(s) 25
FatI CATG 2 cut(s) 291, 800
FbaI TGATCA 1 cut(s) 945
Fnu4HI GCNGC 7 cut(s) 118, 141, 206, 415, 418, 437, 740
Fsp4HI GCNGC 7 cut(s) 118, 141, 206, 415, 418, 437, 740
FspBI CTAG 1 cut(s) 441
GlaI GCGC 4 cut(s) 204, 503, 573, 684
GluI GCNGC 7 cut(s) 118, 141, 206, 415, 418, 437, 740
GsaI CCCAGC 1 cut(s) 630
GsuI CTGGAG 1 cut(s) 889
HaeII RGCGCY 2 cut(s) 206, 575
HaeIII GGCC 2 cut(s) 188, 930
HapII CCGG 5 cut(s) 185, 195, 209, 248, 620
HhaI GCGC 4 cut(s) 205, 504, 574, 685
Hin1I GRCGYC 3 cut(s) 17, 203, 525
Hin1II CATG 2 cut(s) 295, 804
Hin6I GCGC 4 cut(s) 203, 502, 572, 683
HinP1I GCGC 4 cut(s) 203, 502, 572, 683
HindIII AAGCTT 2 cut(s) 95, 854
HinfI GANTC 8 cut(s) 125, 181, 326, 398, 515, 616, 655, 871
HpaII CCGG 5 cut(s) 185, 195, 209, 248, 620
HphI GGTGA 4 cut(s) 57, 259, 353, 611
Hpy166II GTNNAC 1 cut(s) 472
Hpy188I TCNGA 8 cut(s) 124, 180, 337, 397, 403, 568, 763, 920
Hpy188III TCNNGA 6 cut(s) 214, 330, 544, 620, 839, 875
Hpy8I GTNNAC 1 cut(s) 472
Hpy99I CGWCG 7 cut(s) 19, 22, 290, 383, 413, 527, 530
HpyAV CCTTC 4 cut(s) 418, 770, 774, 800
HpyCH4III ACNGT 4 cut(s) 341, 386, 476, 496
HpyCH4IV ACGT 2 cut(s) 17, 525
HpyCH4V TGCA 3 cut(s) 4, 589, 671
HpyF10VI GCNNNNNNNGC 4 cut(s) 146, 414, 853, 862
HpyF3I CTNAG 3 cut(s) 351, 762, 952
HpySE526I ACGT 2 cut(s) 17, 525
Hsp92I GRCGYC 3 cut(s) 17, 203, 525
Hsp92II CATG 2 cut(s) 295, 804
HspAI GCGC 4 cut(s) 203, 502, 572, 683
KasI GGCGCC 1 cut(s) 202
Kpn2I TCCGGA 1 cut(s) 619
Ksp22I TGATCA 1 cut(s) 945
Kzo9I GATC 4 cut(s) 332, 563, 920, 945
LmnI GCTCC 3 cut(s) 125, 303, 499
Lsp1109I GCAGC 3 cut(s) 104, 448, 751
LweI GCATC 2 cut(s) 273, 379
MaeI CTAG 1 cut(s) 441
MaeII ACGT 2 cut(s) 17, 525
MaeIII GTNAC 2 cut(s) 302, 362
MalI GATC 4 cut(s) 334, 565, 922, 947
MboI GATC 4 cut(s) 332, 563, 920, 945
MboII GAAGA 2 cut(s) 284, 614
MhlI GDGCHC 2 cut(s) 134, 867
MluCI AATT 3 cut(s) 48, 81, 936
MluI ACGCGT 1 cut(s) 534
Mly113I GGCGCC 1 cut(s) 203
MlyI GAGTC 2 cut(s) 320, 880
MmeI TCCRAC 1 cut(s) 768
MroI TCCGGA 1 cut(s) 619
MroXI GAANNNNTTC 1 cut(s) 97
MseI TTAA 2 cut(s) 23, 660
MspI CCGG 5 cut(s) 185, 195, 209, 248, 620
MspR9I CCNGG 3 cut(s) 248, 249, 636
MvaI CCWGG 1 cut(s) 636
MvnI CGCG 1 cut(s) 536
MwoI GCNNNNNNNGC 4 cut(s) 146, 414, 853, 862
NarI GGCGCC 1 cut(s) 203
NciI CCSGG 2 cut(s) 248, 249
NdeII GATC 4 cut(s) 332, 563, 920, 945
NlaIII CATG 2 cut(s) 295, 804
NlaIV GGNNCC 3 cut(s) 204, 299, 624
NmuCI GTSAC 1 cut(s) 362
PcsI WCGNNNNNNNCGW 1 cut(s) 417
PdmI GAANNNNTTC 1 cut(s) 97
PfeI GAWTC 6 cut(s) 125, 181, 398, 515, 616, 655
PflMI CCANNNNNTGG 1 cut(s) 165
PfoI TCCNGGA 1 cut(s) 634
PkrI GCNGC 7 cut(s) 119, 142, 207, 416, 419, 438, 741
PleI GAGTC 2 cut(s) 320, 879
PluTI GGCGCC 1 cut(s) 206
PpsI GAGTC 2 cut(s) 320, 879
PsiI TTATAA 1 cut(s) 708
Psp6I CCWGG 1 cut(s) 634
PspFI CCCAGC 1 cut(s) 626
PspGI CCWGG 1 cut(s) 634
PspN4I GGNNCC 3 cut(s) 204, 299, 624
PspPI GGNCC 1 cut(s) 928
RsaI GTAC 3 cut(s) 233, 245, 464
RsaNI GTAC 3 cut(s) 232, 244, 463
SaqAI TTAA 2 cut(s) 23, 660
SatI GCNGC 7 cut(s) 118, 141, 206, 415, 418, 437, 740
Sau3AI GATC 4 cut(s) 332, 563, 920, 945
Sau96I GGNCC 1 cut(s) 928
ScaI AGTACT 1 cut(s) 464
SchI GAGTC 2 cut(s) 320, 880
ScrFI CCNGG 3 cut(s) 248, 249, 636
SduI GDGCHC 2 cut(s) 134, 867
SfaNI GCATC 2 cut(s) 273, 379
SfoI GGCGCC 1 cut(s) 204
SmaI CCCGGG 1 cut(s) 249
SmlI CTYRAG 2 cut(s) 458, 895
SmoI CTYRAG 2 cut(s) 458, 895
Sse9I AATT 3 cut(s) 48, 81, 936
SsiI CCGC 9 cut(s) 35, 138, 141, 147, 173, 206, 220, 414, 417
SspDI GGCGCC 1 cut(s) 202
SspMI CTAG 1 cut(s) 441
StyD4I CCNGG 3 cut(s) 246, 247, 634
TaaI ACNGT 4 cut(s) 341, 386, 476, 496
TaiI ACGT 2 cut(s) 20, 528
TaqI TCGA 3 cut(s) 128, 324, 874
TasI AATT 3 cut(s) 48, 81, 936
TatI WGTACW 1 cut(s) 462
TauI GCSGC 4 cut(s) 143, 208, 417, 420
TfiI GAWTC 6 cut(s) 125, 181, 398, 515, 616, 655
Tru1I TTAA 2 cut(s) 23, 660
Tru9I TTAA 2 cut(s) 23, 660
TscAI CASTG 2 cut(s) 391, 872
TseFI GTSAC 1 cut(s) 362
TseI GCWGC 3 cut(s) 117, 436, 739
Tsp45I GTSAC 1 cut(s) 362
TspDTI ATGAA 1 cut(s) 528
TspGWI ACGGA 1 cut(s) 290
TspMI CCCGGG 1 cut(s) 247
TspRI CASTG 2 cut(s) 391, 872
Van91I CCANNNNNTGG 1 cut(s) 165
XmaI CCCGGG 1 cut(s) 247
XmnI GAANNNNTTC 1 cut(s) 97
XspI CTAG 1 cut(s) 441
ZraI GACGTC 2 cut(s) 18, 526
ZrmI AGTACT 1 cut(s) 464
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.