pycom15g08590

peptidyl-tRNA hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0007778
Physical Location & Seq
Reverse (-)
5452255 .. 5454974
2720 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 714 bp
ATGACATCCCTCGCCTTACCTTTACCTTACCCTCATCCTCCGGCTTACAAGCCCACCAAAAATCTCTCTGCTTCTCTCAGAGCACTGAGCAGTTTACTCTTCGTCTTCGTTGCTCTCAACTCAACAAAACCCATGTTTCGAAACGCTTCTCAGCATCCAAACAAGCCACAGAAGCAAGAAAGAGAATGGTTGGCAGTGAGCTTTAAGCCAGAGAACTTTATTCCAGGAGTTGTCATTGGCTTCATTCTTGGGTTGTTGCTGGATTTATCACAGCCCGGTAAGGGTCGATCGAAGAAGAACAATGCTTCGGTAGGCCAGCTCCAACAGCGGAGTTTGGTCACAAGCAATGTCAGTGGTGGTGAAGAACTTAAAATGGTACTGGTAGTTAGACGAGACCTAAAGATGACAACGGGTAAAATTGCATCTCAGTGTGCTCATGCTGCCACTGGCGCGTATGCAGAACTGATGCAAAGCCACAGATCCCTTTTGAGACAGTGGGAGCAACACGGGCAACCCAAAATAGTGGTTGCATGCAAGAATCAACAAGAAATGAATAAGCTGAAGGAAGCAGCTGAGAACATTGGCATTCCAACTTTTGTCGTTGCTGATGCAGGGCGAACACAGGTTGTGAGTGGATCGAAGACAGTTCTTGCCATTGGACCTGGACCAAAAGAATCAGTAGATTCAGTGACAGGGAAACAGGCTCTTCTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 map03013
Pfam Domains
Protein Families
PTH2

Protein Analysis

238

Amino Acids

25.67

Weight (kDa)

10.01

Isoelectric Point (pI)

40.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PTH2 PF01981 123 - 236 1.4e-44 Peptidyl-tRNA hydrolase PTH2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 452
AciI CCGC 1 cut(s) 328
AclWI GGATC 2 cut(s) 474, 643
AcuI CTGAAG 1 cut(s) 581
AfaI GTAC 1 cut(s) 378
AfiI CCNNNNNNNGG 1 cut(s) 281
AjnI CCWGG 2 cut(s) 223, 661
AluBI AGCT 4 cut(s) 201, 319, 559, 572
AluI AGCT 4 cut(s) 201, 319, 559, 572
Alw21I GWGCWC 2 cut(s) 85, 436
Alw26I GTCTC 2 cut(s) 387, 484
AlwI GGATC 2 cut(s) 474, 643
AoxI GGCC 1 cut(s) 313
ApeKI GCWGC 2 cut(s) 440, 569
Asp700I GAANNNNTTC 1 cut(s) 145
AspLEI GCGC 1 cut(s) 452
AspS9I GGNCC 2 cut(s) 659, 665
AsuC2I CCSGG 1 cut(s) 276
AsuHPI GGTGA 1 cut(s) 371
AsuII TTCGAA 1 cut(s) 139
AvaII GGWCC 2 cut(s) 659, 665
BbsI GAAGAC 2 cut(s) 97, 647
Bbv12I GWGCWC 2 cut(s) 85, 436
BbvI GCAGC 2 cut(s) 427, 581
BciT130I CCWGG 2 cut(s) 225, 663
BcnI CCSGG 1 cut(s) 276
BcoDI GTCTC 2 cut(s) 387, 484
BisI GCNGC 2 cut(s) 441, 570
BlsI GCNGC 2 cut(s) 442, 571
Bme1390I CCNGG 3 cut(s) 225, 276, 663
Bme18I GGWCC 2 cut(s) 659, 665
BmgT120I GGNCC 2 cut(s) 659, 665
BmrFI CCNGG 3 cut(s) 225, 276, 663
BmsI GCATC 4 cut(s) 163, 431, 456, 598
BpiI GAAGAC 2 cut(s) 97, 647
Bpu14I TTCGAA 1 cut(s) 139
BpuMI CCSGG 1 cut(s) 276
BsaI GGTCTC 1 cut(s) 387
Bsc4I CCNNNNNNNGG 1 cut(s) 281
Bse1I ACTGG 2 cut(s) 384, 451
Bse3DI GCAATG 1 cut(s) 352
BseBI CCWGG 2 cut(s) 225, 663
BseGI GGATG 3 cut(s) 5, 34, 154
BseLI CCNNNNNNNGG 1 cut(s) 281
BseMI GCAATG 1 cut(s) 352
BseMII CTCAG 5 cut(s) 77, 91, 164, 440, 564
BseNI ACTGG 2 cut(s) 384, 451
BseXI GCAGC 2 cut(s) 427, 581
Bsh1236I CGCG 1 cut(s) 452
Bsh1285I CGRYCG 1 cut(s) 290
BshFI GGCC 1 cut(s) 315
BsiEI CGRYCG 1 cut(s) 290
BsiHKAI GWGCWC 2 cut(s) 85, 436
BsiSI CCGG 2 cut(s) 41, 276
BslI CCNNNNNNNGG 1 cut(s) 281
BsmAI GTCTC 2 cut(s) 387, 484
BsmI GAATGC 1 cut(s) 585
BsnI GGCC 1 cut(s) 315
Bso31I GGTCTC 1 cut(s) 387
Bsp119I TTCGAA 1 cut(s) 139
Bsp1286I GDGCHC 2 cut(s) 85, 436
Bsp143I GATC 3 cut(s) 287, 479, 635
BspACI CCGC 1 cut(s) 328
BspANI GGCC 1 cut(s) 315
BspCNI CTCAG 5 cut(s) 78, 90, 163, 439, 565
BspFNI CGCG 1 cut(s) 452
BspPI GGATC 2 cut(s) 474, 643
BspQI GCTCTTC 1 cut(s) 711
BspT104I TTCGAA 1 cut(s) 139
BspTNI GGTCTC 1 cut(s) 387
BsrDI GCAATG 1 cut(s) 352
BsrI ACTGG 2 cut(s) 384, 451
BssMI GATC 3 cut(s) 287, 479, 635
Bst2UI CCWGG 2 cut(s) 225, 663
Bst4CI ACNGT 2 cut(s) 495, 646
Bst6I CTCTTC 2 cut(s) 104, 711
BstBI TTCGAA 1 cut(s) 139
BstC8I GCNNGC 2 cut(s) 317, 532
BstDEI CTNAG 5 cut(s) 77, 86, 150, 426, 573
BstF5I GGATG 3 cut(s) 5, 34, 154
BstFNI CGCG 1 cut(s) 452
BstHHI GCGC 1 cut(s) 452
BstKTI GATC 3 cut(s) 290, 482, 638
BstMAI GTCTC 2 cut(s) 387, 484
BstMBI GATC 3 cut(s) 287, 479, 635
BstMCI CGRYCG 1 cut(s) 290
BstMWI GCNNNNNNNGC 5 cut(s) 172, 325, 440, 449, 508
BstNI CCWGG 2 cut(s) 225, 663
BstNSI RCATGY 1 cut(s) 534
BstSCI CCNGG 3 cut(s) 223, 274, 661
BstUI CGCG 1 cut(s) 452
BstV1I GCAGC 2 cut(s) 427, 581
BstV2I GAAGAC 2 cut(s) 97, 647
BstX2I RGATCY 1 cut(s) 479
BstXI CCANNNNNNTGG 1 cut(s) 523
BstYI RGATCY 1 cut(s) 479
BsuRI GGCC 1 cut(s) 315
BtsCI GGATG 3 cut(s) 5, 34, 154
BtsI GCAGTG 1 cut(s) 201
BtsIMutI CAGTG 7 cut(s) 83, 201, 358, 434, 444, 500, 693
Cac8I GCNNGC 2 cut(s) 317, 532
CfoI GCGC 1 cut(s) 452
Cfr13I GGNCC 2 cut(s) 659, 665
Csp6I GTAC 1 cut(s) 377
CspCI CAANNNNNGTGG 2 cut(s) 334, 369
CviAII CATG 3 cut(s) 133, 437, 531
CviQI GTAC 1 cut(s) 377
DdeI CTNAG 5 cut(s) 77, 86, 150, 426, 573
DpnI GATC 3 cut(s) 289, 481, 637
DpnII GATC 3 cut(s) 287, 479, 635
Eam1104I CTCTTC 2 cut(s) 104, 711
EarI CTCTTC 2 cut(s) 104, 711
Eco31I GGTCTC 1 cut(s) 387
Eco47I GGWCC 2 cut(s) 659, 665
Eco57I CTGAAG 1 cut(s) 581
EcoRII CCWGG 2 cut(s) 223, 661
FaeI CATG 3 cut(s) 136, 440, 534
FaiI YATR 5 cut(s) 134, 438, 456, 532, 712
FatI CATG 3 cut(s) 132, 436, 530
Fnu4HI GCNGC 2 cut(s) 441, 570
FokI GGATG 2 cut(s) 21, 141
Fsp4HI GCNGC 2 cut(s) 441, 570
GlaI GCGC 1 cut(s) 451
GluI GCNGC 2 cut(s) 441, 570
HaeIII GGCC 1 cut(s) 315
HapII CCGG 2 cut(s) 41, 276
HhaI GCGC 1 cut(s) 452
Hin1II CATG 3 cut(s) 136, 440, 534
Hin6I GCGC 1 cut(s) 450
HinP1I GCGC 1 cut(s) 450
HinfI GANTC 3 cut(s) 538, 674, 683
HpaII CCGG 2 cut(s) 41, 276
HphI GGTGA 1 cut(s) 371
Hpy166II GTNNAC 1 cut(s) 95
Hpy188I TCNGA 1 cut(s) 80
Hpy8I GTNNAC 1 cut(s) 95
HpyAV CCTTC 1 cut(s) 556
HpyCH4III ACNGT 2 cut(s) 495, 646
HpyCH4V TGCA 6 cut(s) 422, 458, 469, 530, 534, 611
HpyF10VI GCNNNNNNNGC 5 cut(s) 172, 325, 440, 449, 508
HpyF3I CTNAG 5 cut(s) 77, 86, 150, 426, 573
Hsp92II CATG 3 cut(s) 136, 440, 534
HspAI GCGC 1 cut(s) 450
Kzo9I GATC 3 cut(s) 287, 479, 635
LguI GCTCTTC 1 cut(s) 711
LmnI GCTCC 2 cut(s) 324, 499
Lsp1109I GCAGC 2 cut(s) 427, 581
LweI GCATC 4 cut(s) 163, 431, 456, 598
MaeIII GTNAC 2 cut(s) 337, 688
MalI GATC 3 cut(s) 289, 481, 637
MboI GATC 3 cut(s) 287, 479, 635
MboII GAAGA 7 cut(s) 91, 97, 304, 307, 374, 652, 698
MflI RGATCY 1 cut(s) 479
MhlI GDGCHC 2 cut(s) 85, 436
MluCI AATT 1 cut(s) 417
MmeI TCCRAC 2 cut(s) 346, 614
MnlI CCTC 3 cut(s) 20, 42, 48
MroXI GAANNNNTTC 1 cut(s) 145
MseI TTAA 2 cut(s) 204, 369
MslI CAYNNNNRTG 1 cut(s) 427
MspA1I CMGCKG 2 cut(s) 328, 572
MspI CCGG 2 cut(s) 41, 276
MspR9I CCNGG 3 cut(s) 225, 276, 663
Mva1269I GAATGC 1 cut(s) 585
MvaI CCWGG 2 cut(s) 225, 663
MvnI CGCG 1 cut(s) 452
MwoI GCNNNNNNNGC 5 cut(s) 172, 325, 440, 449, 508
NciI CCSGG 1 cut(s) 276
NdeII GATC 3 cut(s) 287, 479, 635
NlaIII CATG 3 cut(s) 136, 440, 534
NmuCI GTSAC 2 cut(s) 337, 688
NspI RCATGY 1 cut(s) 534
NspV TTCGAA 1 cut(s) 139
PaeI GCATGC 1 cut(s) 534
PciSI GCTCTTC 1 cut(s) 711
PctI GAATGC 1 cut(s) 585
PdmI GAANNNNTTC 1 cut(s) 145
PfeI GAWTC 3 cut(s) 538, 674, 683
PfoI TCCNGGA 1 cut(s) 223
PkrI GCNGC 2 cut(s) 442, 571
Ple19I CGATCG 1 cut(s) 290
Psp6I CCWGG 2 cut(s) 223, 661
PspGI CCWGG 2 cut(s) 223, 661
PspPI GGNCC 2 cut(s) 659, 665
PsuI RGATCY 1 cut(s) 479
PvuI CGATCG 1 cut(s) 290
PvuII CAGCTG 1 cut(s) 572
RsaI GTAC 1 cut(s) 378
RsaNI GTAC 1 cut(s) 377
RseI CAYNNNNRTG 1 cut(s) 427
SapI GCTCTTC 1 cut(s) 711
SaqAI TTAA 2 cut(s) 204, 369
SatI GCNGC 2 cut(s) 441, 570
Sau3AI GATC 3 cut(s) 287, 479, 635
Sau96I GGNCC 2 cut(s) 659, 665
ScrFI CCNGG 3 cut(s) 225, 276, 663
SduI GDGCHC 2 cut(s) 85, 436
SetI ASST 9 cut(s) 22, 28, 203, 321, 399, 561, 574, 627, 664
SfaNI GCATC 4 cut(s) 163, 431, 456, 598
SfuI TTCGAA 1 cut(s) 139
SinI GGWCC 2 cut(s) 659, 665
SmiMI CAYNNNNRTG 1 cut(s) 427
SphI GCATGC 1 cut(s) 534
Sse9I AATT 1 cut(s) 417
SsiI CCGC 1 cut(s) 328
StyD4I CCNGG 3 cut(s) 223, 274, 661
TaaI ACNGT 2 cut(s) 495, 646
TaqI TCGA 4 cut(s) 139, 286, 290, 638
TasI AATT 1 cut(s) 417
TfiI GAWTC 3 cut(s) 538, 674, 683
Tru1I TTAA 2 cut(s) 204, 369
Tru9I TTAA 2 cut(s) 204, 369
TscAI CASTG 7 cut(s) 90, 201, 358, 434, 451, 500, 693
TseFI GTSAC 2 cut(s) 337, 688
TseI GCWGC 2 cut(s) 440, 569
Tsp45I GTSAC 2 cut(s) 337, 688
TspDTI ATGAA 2 cut(s) 232, 566
TspRI CASTG 7 cut(s) 90, 201, 358, 434, 451, 500, 693
VpaK11BI GGWCC 2 cut(s) 659, 665
XceI RCATGY 1 cut(s) 534
XmnI GAANNNNTTC 1 cut(s) 145
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.