pycom15g09850

domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0005356
Physical Location & Seq
Reverse (-)
6404054 .. 6405373
1320 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1320 bp
ATGGCGACAAGATCTAAAGATCGTAGCCCTACTCCAGGGAGGGAGAAGAAGAGCACATCATCCCCATCAACCACCAAAAGGCTCACCAAGTCCCAAACTACAGTCACTGAAAAATCCTTGTCAACCTCATCAGCAAAACAAGTCCCAAACTATCTCAGGCCGACGTTAAGCGCCTCGAGGCACGAAAATTCGCTGAGGTCATCGTCCGCCGGTGCCAAGAAACATGGCCCTGAAGATCATGCAGCATCTCAAAACCCTACATTGAATAGAAGAAGATCTTTTGACAAGCCACCATCAGCTTTTAGGATTCAGAAGGCATTGATCTCCCCAGGAAGGGACGCGCCACTGCGATCTTCGTCATTTTCAGCGAAAATCTCCTCTAATGCTCCGAGACCAAGAATAGAGAGGACGAAATCGAGCACTTTTGGGTCTGGAAAGCCGCAGCTTTTGCCTGCAAAGAGTGTGAAGAAGAGCAGCACAAGCAGTTCGGTTAAGAAGGAGAGTTCATCGGGTTCAACAAGAGCTCCGAAGAGCAGGGACATAACGCATACACTTAATCTCGAAGCGAATTTAGATGCTATAGTGTCTTTGGAACATCATGAGGTTGAAGAGGTAGGTAAGATTTCGACTGAGGAGCATGATAAAGTGATTCTTCCGGATCCAAAAGGCGAAGAGAGTGAGCACGTTGGTGATCATGATGCTGGTGTTTCTGAGGCTGAAGCAAATAGCGGCGAGGATGAGAAGGTGAAGGCTAGTGAGGGTTCAAATGGTAATACGGAGGAATTGAAGGAAAATGTGGAGGAAAGAAACGAAGGAAGCGGAACTGTATTGACCGAAAAAGAAGAGAAGCCAGGGGATAATGATCAACAAGATGGGGAAGAGAATGCAAAAAAAAAAGATGACAGCGGCGAGACAAAGGCGGAAGAGGGTGTGGCAAGTGAAGATCTGGAAACAAAAGTTGAGATGAAAGAAGAACAGGAAAAAGAGAGTGATAGTAAAGAAGAAAATGAAGGGGAGGAAAGCAAAAATGTTGAGGAAAAGGCATTGGATGAGAAGGAAAGAGAAGTGGTTGTGGATGGTGGAGTTGAGGAAGCAAAGCCAACGGAGGCAGGTGAGGCGGCTGTTGAGGTGGCAGAGGAAACACTAAAGCCGCGACAAGCGAGCGGGGGATCTTCACAAGGAGGAGGGAAGAAAGACTCAGCTCCTGCATACAATGATGTGATTGAGGAGACTACAAACAAGTTGATGGAGAAAAGAAAGAACAAGGTGAAGGCATTGGTTGGGGCCTTTGAAACTGTCATAGATTATGAATCCAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CaM_binding

Protein Analysis

440

Amino Acids

47.48

Weight (kDa)

5.33

Isoelectric Point (pI)

57.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CaM_binding PF07839 323 - 434 4.1e-09 Plant calmodulin-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1100
AbsI CCTCGAGG 1 cut(s) 175
Acc36I ACCTGC 1 cut(s) 1100
AccB1I GGYRCC 1 cut(s) 212
AccBSI CCGCTC 1 cut(s) 1164
AccII CGCG 2 cut(s) 341, 1153
AccIII TCCGGA 1 cut(s) 655
AciI CCGC 9 cut(s) 207, 440, 729, 819, 906, 920, 1118, 1151, 1164
AclWI GGATC 3 cut(s) 653, 666, 1177
AcsI RAATTY 2 cut(s) 187, 568
AcuI CTGAAG 2 cut(s) 252, 738
AfiI CCNNNNNNNGG 4 cut(s) 35, 78, 333, 334
AgsI TTSAA 6 cut(s) 265, 516, 608, 765, 787, 1292
AjnI CCWGG 3 cut(s) 34, 328, 850
AleI CACNNNNGTG 1 cut(s) 687
AluBI AGCT 4 cut(s) 299, 445, 524, 1202
AluI AGCT 4 cut(s) 299, 445, 524, 1202
Alw21I GWGCWC 4 cut(s) 56, 422, 526, 684
Alw26I GTCTC 3 cut(s) 385, 905, 1223
AlwI GGATC 3 cut(s) 653, 666, 1177
AlwNI CAGNNNCTG 2 cut(s) 107, 1205
Ama87I CYCGRG 1 cut(s) 175
Aor13HI TCCGGA 1 cut(s) 655
AoxI GGCC 3 cut(s) 158, 226, 1284
ApeKI GCWGC 3 cut(s) 242, 442, 474
ApoI RAATTY 2 cut(s) 187, 568
AspLEI GCGC 2 cut(s) 173, 343
AspS9I GGNCC 2 cut(s) 227, 1284
AsuHPI GGTGA 5 cut(s) 76, 701, 757, 1124, 1279
AvaI CYCGRG 1 cut(s) 175
BamHI GGATCC 1 cut(s) 658
BanI GGYRCC 1 cut(s) 212
BanII GRGCYC 1 cut(s) 526
Bbv12I GWGCWC 4 cut(s) 56, 422, 526, 684
BbvCI CCTCAGC 1 cut(s) 194
BbvI GCAGC 3 cut(s) 254, 454, 486
BccI CCATC 5 cut(s) 73, 301, 866, 1070, 1240
BciT130I CCWGG 3 cut(s) 36, 330, 852
BclI TGATCA 2 cut(s) 691, 862
BcoDI GTCTC 3 cut(s) 385, 905, 1223
BfaI CTAG 1 cut(s) 753
BfmI CTRYAG 2 cut(s) 99, 579
BfoI RGCGCY 1 cut(s) 174
BfuAI ACCTGC 1 cut(s) 1100
BglII AGATCT 3 cut(s) 11, 275, 943
BisI GCNGC 8 cut(s) 243, 440, 443, 475, 730, 907, 1119, 1151
BlsI GCNGC 8 cut(s) 244, 441, 444, 476, 731, 908, 1120, 1152
Bme1390I CCNGG 3 cut(s) 36, 330, 852
BmeT110I CYCGRG 1 cut(s) 175
BmgT120I GGNCC 2 cut(s) 227, 1284
BmiI GGNNCC 3 cut(s) 214, 660, 1285
BmrFI CCNGG 3 cut(s) 36, 330, 852
BmsI GCATC 3 cut(s) 254, 565, 688
BpmI CTGGAG 1 cut(s) 18
Bpu10I CCTNAGC 1 cut(s) 194
BsaBI GATNNNNATC 1 cut(s) 861
BsaI GGTCTC 1 cut(s) 385
BsaJI CCNNGG 3 cut(s) 35, 328, 851
BsaWI WCCGGW 1 cut(s) 655
BsaXI ACNNNNNCTCC 2 cut(s) 508, 538
Bsc4I CCNNNNNNNGG 4 cut(s) 35, 78, 333, 334
Bse118I RCCGGY 1 cut(s) 209
Bse8I GATNNNNATC 1 cut(s) 861
BseAI TCCGGA 1 cut(s) 655
BseBI CCWGG 3 cut(s) 36, 330, 852
BseDI CCNNGG 3 cut(s) 35, 328, 851
BseGI GGATG 4 cut(s) 59, 742, 1054, 1081
BseJI GATNNNNATC 1 cut(s) 861
BseLI CCNNNNNNNGG 4 cut(s) 35, 78, 333, 334
BseMII CTCAG 5 cut(s) 169, 185, 621, 702, 1212
BseRI GAGGAG 4 cut(s) 367, 647, 1197, 1241
BseXI GCAGC 3 cut(s) 254, 454, 486
Bsh1236I CGCG 2 cut(s) 341, 1153
BshFI GGCC 3 cut(s) 160, 228, 1286
BshNI GGYRCC 1 cut(s) 212
BsiHKAI GWGCWC 4 cut(s) 56, 422, 526, 684
BsiHKCI CYCGRG 1 cut(s) 175
BsiSI CCGG 2 cut(s) 210, 656
BslFI GGGAC 4 cut(s) 76, 128, 350, 551
BslI CCNNNNNNNGG 4 cut(s) 35, 78, 333, 334
BsmAI GTCTC 3 cut(s) 385, 905, 1223
BsmFI GGGAC 4 cut(s) 76, 128, 350, 551
BsmI GAATGC 1 cut(s) 889
BsnI GGCC 3 cut(s) 160, 228, 1286
Bso31I GGTCTC 1 cut(s) 385
BsoBI CYCGRG 1 cut(s) 175
Bsp1286I GDGCHC 4 cut(s) 56, 422, 526, 684
Bsp13I TCCGGA 1 cut(s) 655
BspACI CCGC 9 cut(s) 207, 440, 729, 819, 906, 920, 1118, 1151, 1164
BspANI GGCC 3 cut(s) 160, 228, 1286
BspCNI CTCAG 5 cut(s) 168, 186, 622, 703, 1211
BspEI TCCGGA 1 cut(s) 655
BspFNI CGCG 2 cut(s) 341, 1153
BspHI TCATGA 2 cut(s) 598, 694
BspLI GGNNCC 3 cut(s) 214, 660, 1285
BspMI ACCTGC 1 cut(s) 1100
BspPI GGATC 3 cut(s) 653, 666, 1177
BspQI GCTCTTC 3 cut(s) 44, 464, 524
BspT107I GGYRCC 1 cut(s) 212
BspTNI GGTCTC 1 cut(s) 385
BsrBI CCGCTC 1 cut(s) 1164
BsrFI RCCGGY 1 cut(s) 209
BssAI RCCGGY 1 cut(s) 209
BssECI CCNNGG 3 cut(s) 35, 328, 851
Bst2UI CCWGG 3 cut(s) 36, 330, 852
Bst4CI ACNGT 3 cut(s) 103, 826, 1297
Bst6I CTCTTC 8 cut(s) 44, 464, 524, 603, 666, 837, 873, 918
BstAPI GCANNNNNTGC 1 cut(s) 448
BstC8I GCNNGC 2 cut(s) 453, 1162
BstDEI CTNAG 5 cut(s) 155, 194, 630, 711, 1198
BstENI CCTNNNNNAGG 1 cut(s) 33
BstF5I GGATG 4 cut(s) 59, 742, 1054, 1081
BstFNI CGCG 2 cut(s) 341, 1153
BstH2I RGCGCY 1 cut(s) 174
BstHHI GCGC 2 cut(s) 173, 343
BstMAI GTCTC 3 cut(s) 385, 905, 1223
BstMWI GCNNNNNNNGC 3 cut(s) 448, 480, 1115
BstNI CCWGG 3 cut(s) 36, 330, 852
BstSCI CCNGG 3 cut(s) 34, 328, 850
BstSFI CTRYAG 2 cut(s) 99, 579
BstUI CGCG 2 cut(s) 341, 1153
BstV1I GCAGC 3 cut(s) 254, 454, 486
BstX2I RGATCY 5 cut(s) 11, 275, 658, 943, 1169
BstYI RGATCY 5 cut(s) 11, 275, 658, 943, 1169
BsuRI GGCC 3 cut(s) 160, 228, 1286
BtsCI GGATG 4 cut(s) 59, 742, 1054, 1081
BtsI GCAGTG 1 cut(s) 344
BtsIMutI CAGTG 2 cut(s) 105, 344
BveI ACCTGC 1 cut(s) 1100
Cac8I GCNNGC 2 cut(s) 453, 1162
CaiI CAGNNNCTG 2 cut(s) 107, 1205
CciI TCATGA 2 cut(s) 598, 694
CfoI GCGC 2 cut(s) 173, 343
Cfr10I RCCGGY 1 cut(s) 209
Cfr13I GGNCC 2 cut(s) 227, 1284
CseI GACGC 1 cut(s) 347
CviAII CATG 5 cut(s) 224, 239, 599, 638, 695
DdeI CTNAG 5 cut(s) 155, 194, 630, 711, 1198
Eam1104I CTCTTC 8 cut(s) 44, 464, 524, 603, 666, 837, 873, 918
EarI CTCTTC 8 cut(s) 44, 464, 524, 603, 666, 837, 873, 918
EciI GGCGGA 2 cut(s) 196, 935
Ecl136II GAGCTC 1 cut(s) 524
Eco24I GRGCYC 1 cut(s) 526
Eco31I GGTCTC 1 cut(s) 385
Eco53kI GAGCTC 1 cut(s) 524
Eco57I CTGAAG 2 cut(s) 252, 738
Eco88I CYCGRG 1 cut(s) 175
EcoICRI GAGCTC 1 cut(s) 524
EcoNI CCTNNNNNAGG 1 cut(s) 33
EcoO109I RGGNCCY 1 cut(s) 1284
EcoRII CCWGG 3 cut(s) 34, 328, 850
EcoT38I GRGCYC 1 cut(s) 526
FaeI CATG 5 cut(s) 227, 242, 602, 641, 698
FalI AAGNNNNNCTT 4 cut(s) 262, 294, 636, 668
FaqI GGGAC 4 cut(s) 76, 128, 350, 551
FatI CATG 5 cut(s) 223, 238, 598, 637, 694
FauI CCCGC 1 cut(s) 1157
FbaI TGATCA 2 cut(s) 691, 862
Fnu4HI GCNGC 8 cut(s) 243, 440, 443, 475, 730, 907, 1119, 1151
FokI GGATG 4 cut(s) 46, 749, 1061, 1088
FriOI GRGCYC 1 cut(s) 526
Fsp4HI GCNGC 8 cut(s) 243, 440, 443, 475, 730, 907, 1119, 1151
FspBI CTAG 1 cut(s) 753
GlaI GCGC 2 cut(s) 172, 342
GluI GCNGC 8 cut(s) 243, 440, 443, 475, 730, 907, 1119, 1151
GsuI CTGGAG 1 cut(s) 18
HaeII RGCGCY 1 cut(s) 174
HaeIII GGCC 3 cut(s) 160, 228, 1286
HapII CCGG 2 cut(s) 210, 656
HgaI GACGC 1 cut(s) 347
HhaI GCGC 2 cut(s) 173, 343
Hin1II CATG 5 cut(s) 227, 242, 602, 641, 698
Hin6I GCGC 2 cut(s) 171, 341
HinP1I GCGC 2 cut(s) 171, 341
HincII GTYRAC 1 cut(s) 123
HindII GTYRAC 1 cut(s) 123
HinfI GANTC 4 cut(s) 307, 649, 1196, 1310
HpaII CCGG 2 cut(s) 210, 656
HphI GGTGA 5 cut(s) 76, 701, 757, 1124, 1279
Hpy166II GTNNAC 1 cut(s) 123
Hpy188I TCNGA 4 cut(s) 312, 390, 528, 712
Hpy188III TCNNGA 6 cut(s) 432, 560, 599, 656, 695, 947
Hpy8I GTNNAC 1 cut(s) 123
Hpy99I CGWCG 1 cut(s) 166
HpyCH4III ACNGT 3 cut(s) 103, 826, 1297
HpyCH4IV ACGT 2 cut(s) 164, 684
HpyCH4V TGCA 4 cut(s) 242, 455, 887, 1208
HpyF10VI GCNNNNNNNGC 3 cut(s) 448, 480, 1115
HpyF3I CTNAG 5 cut(s) 155, 194, 630, 711, 1198
HpySE526I ACGT 2 cut(s) 164, 684
Hsp92II CATG 5 cut(s) 227, 242, 602, 641, 698
HspAI GCGC 2 cut(s) 171, 341
Kpn2I TCCGGA 1 cut(s) 655
Ksp22I TGATCA 2 cut(s) 691, 862
LguI GCTCTTC 3 cut(s) 44, 464, 524
LmnI GCTCC 4 cut(s) 391, 529, 634, 1207
Lsp1109I GCAGC 3 cut(s) 254, 454, 486
LweI GCATC 3 cut(s) 254, 565, 688
MaeI CTAG 1 cut(s) 753
MaeII ACGT 2 cut(s) 164, 684
MaeIII GTNAC 1 cut(s) 103
MbiI CCGCTC 1 cut(s) 1164
MflI RGATCY 5 cut(s) 11, 275, 658, 943, 1169
MhlI GDGCHC 4 cut(s) 56, 422, 526, 684
MluCI AATT 3 cut(s) 187, 568, 782
MlyI GAGTC 1 cut(s) 1190
MroI TCCGGA 1 cut(s) 655
MseI TTAA 3 cut(s) 167, 492, 555
MslI CAYNNNNRTG 1 cut(s) 687
MspA1I CMGCKG 1 cut(s) 906
MspI CCGG 2 cut(s) 210, 656
MspR9I CCNGG 3 cut(s) 36, 330, 852
Mva1269I GAATGC 1 cut(s) 889
MvaI CCWGG 3 cut(s) 36, 330, 852
MvnI CGCG 2 cut(s) 341, 1153
MwoI GCNNNNNNNGC 3 cut(s) 448, 480, 1115
NlaIII CATG 5 cut(s) 227, 242, 602, 641, 698
NlaIV GGNNCC 3 cut(s) 214, 660, 1285
NmuCI GTSAC 1 cut(s) 103
OliI CACNNNNGTG 1 cut(s) 687
PaeR7I CTCGAG 1 cut(s) 175
PagI TCATGA 2 cut(s) 598, 694
PaqCI CACCTGC 1 cut(s) 1100
PciSI GCTCTTC 3 cut(s) 44, 464, 524
PctI GAATGC 1 cut(s) 889
PfeI GAWTC 3 cut(s) 307, 649, 1310
PkrI GCNGC 8 cut(s) 244, 441, 444, 476, 731, 908, 1120, 1152
PleI GAGTC 1 cut(s) 1190
PpsI GAGTC 1 cut(s) 1190
Psp124BI GAGCTC 1 cut(s) 526
Psp6I CCWGG 3 cut(s) 34, 328, 850
PspGI CCWGG 3 cut(s) 34, 328, 850
PspN4I GGNNCC 3 cut(s) 214, 660, 1285
PspPI GGNCC 2 cut(s) 227, 1284
PspXI VCTCGAGB 1 cut(s) 175
PstNI CAGNNNCTG 2 cut(s) 107, 1205
PsuI RGATCY 5 cut(s) 11, 275, 658, 943, 1169
RseI CAYNNNNRTG 1 cut(s) 687
SacI GAGCTC 1 cut(s) 526
SapI GCTCTTC 3 cut(s) 44, 464, 524
SaqAI TTAA 3 cut(s) 167, 492, 555
SatI GCNGC 8 cut(s) 243, 440, 443, 475, 730, 907, 1119, 1151
Sau96I GGNCC 2 cut(s) 227, 1284
SchI GAGTC 1 cut(s) 1190
ScrFI CCNGG 3 cut(s) 36, 330, 852
SduI GDGCHC 4 cut(s) 56, 422, 526, 684
SfaNI GCATC 3 cut(s) 254, 565, 688
SfcI CTRYAG 2 cut(s) 99, 579
Sfr274I CTCGAG 1 cut(s) 175
SgrAI CRCCGGYG 1 cut(s) 209
SlaI CTCGAG 1 cut(s) 175
SmiMI CAYNNNNRTG 1 cut(s) 687
SmlI CTYRAG 1 cut(s) 175
SmoI CTYRAG 1 cut(s) 175
Sse9I AATT 3 cut(s) 187, 568, 782
SsiI CCGC 9 cut(s) 207, 440, 729, 819, 906, 920, 1118, 1151, 1164
SspMI CTAG 1 cut(s) 753
SstI GAGCTC 1 cut(s) 526
StyD4I CCNGG 3 cut(s) 34, 328, 850
TaaI ACNGT 3 cut(s) 103, 826, 1297
TaiI ACGT 2 cut(s) 167, 687
TaqI TCGA 4 cut(s) 176, 416, 561, 626
TaqII GACCGA 1 cut(s) 848
TasI AATT 3 cut(s) 187, 568, 782
TauI GCSGC 5 cut(s) 442, 732, 909, 1121, 1153
TfiI GAWTC 3 cut(s) 307, 649, 1310
Tru1I TTAA 3 cut(s) 167, 492, 555
Tru9I TTAA 3 cut(s) 167, 492, 555
TscAI CASTG 2 cut(s) 112, 351
TseFI GTSAC 1 cut(s) 103
TseI GCWGC 3 cut(s) 242, 442, 474
Tsp45I GTSAC 1 cut(s) 103
TspDTI ATGAA 3 cut(s) 495, 980, 1023
TspGWI ACGGA 2 cut(s) 791, 1118
TspRI CASTG 2 cut(s) 112, 351
XagI CCTNNNNNAGG 1 cut(s) 33
XapI RAATTY 2 cut(s) 187, 568
XhoI CTCGAG 1 cut(s) 175
XspI CTAG 1 cut(s) 753
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.