pycom15g13900

atfip1 v ,atfips5,fip1 v ,fips5

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0009289
Physical Location & Seq
Forward (+)
9475145 .. 9482565
7421 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 4092 bp
ATGGAAGACGACGACGAATTCGGAGATCTCTACACAGACGTTCTTCGACCCTTTGAATCGTCCCAACCATCATCATCATCATCTGCGCCGCTGCCCCACCAATCCTCCGCCGCACCCCAATCTTTCCACCGTCCGATCGATGTCAATGTCCCCGACGAAGAGGACGAGATCCTTTTCGCAGCTCCTCACTCTAATCCCTCTGTCTCTCACCCGTCCAATTCCCAAACCCTAGCTCCCGCCGCCTCTGTTCCCACCAATTCGGCTAGAGATGCCGCTCCGGCTGGCGGGTCTAGGGTTTTGGAGGCTAAAGATGTCGAATTGCCGAAAGTCAATTCCGTGGATTTGAATATTGGTGGTAAAGATTTGGATTTGATGGACAAGGATGTGAATTTCGACATCGAGGAGGCTAACAATGGAGCTGATGCTATGGGTTTGGACCCGGTGATTCCCGGGCTATCGGATACCTTTCCAATCACTGGTGCTGCGGTGAATATCGGCAACCCAGAAGGTTCAAGAAGAGATGGTGAGAGAGGCGAAGATGATTGGGACAGCGACGATAGCGACGACGATTTGCAGATAGTGTTGAATGATAACAACCACGGGCCTATGGCTATGGAGAGAGGTGGAATGGGCGGCGAAGATGAAGATGACGATGACGGGCTCGTTATTGTGGCTGATGGTGAGCCAAACCAGCCAATGGACGACCAAGAGTGGGGCGAAGAGAGCGCTCAGACAGCGGAAGGCGAGAGGAAGGAGATGGGTGAAGCCGGGAAGACAGCTGCCGGCGGCGTGGTTGTGCCCCCAAAAGTTGGGTACAGCAGTCATGGGTATCATCCATTTCATTCTCAGTTTAAGTACGTAAGACCTGGCGCTGTGCCAATGCCTGGGCCAACTACTTCTGGCCCTGGAGGAGTCCCAGGTCAAGTTCGTCCACTTTTAAACATGGGTCCTGTACCTGGCCGAGGTAGAGGTGACTGGAGACCAACAGGAATGAGAGATGGCACTCCTCTGCAGAAGAACTTTCATTCAGGCTTTGGTACGCCTGGCTGGGGCAACAATACGGGAGGTCGTGGTTTCGGTGGTGGACTGGAGTTCACACTTCCTTCACACAAGACCATATTTGATGTCGACATTGATGGTTTTGAGGAAAAACCTTGGAAATATCCTGGTGTTGATACATCGGACTTTTTCAACTTTGGCTTAAATGAAGATAGCTGGAAAGATTATTGCAAACAGCTGGAACTGCTTCGCTTGGAGTCCACCATGCAAAGCAAAATTCGTGTTTACGAAAGTGGACGAACAGAACAGGAGTATGATCCAGATCTGCCCCCAGAGCTTGCAGCGGCAACTGGTATTCATGAACTTCCTCCTGAAAATGCAAACCCTGGGAAGTCAGATGTTGTCCAAAGTGATTTAGTAAAAGGAACTGCTCGTCTGCGTCCTCCTATTCCAACTGGAAGAGCAATACAGGTGGAAGGCGGTTTTGGTGAGCGTCTCCCCTCGATTGATACCCGACCACCTCGGGTTCGTGATTCAGATGCGATCATTGAGATTGTCTTGCAGGACTCTTTAGATGATGATTCCTCTGCAGGAAATGGTATCCCAGACGGAGTTGAAAATGATCACCCAAGAGAGGGTTTTGGTAGAGGTGAAGGACACCTTGCACAGGTTGAGAGTGAGTACTTTGATGGTTTGCCCCAGGCTTATAACGATAGAAAGAGGGAGTTGGTAGGAAGAAAAATGCCTTTCCACGATGACATACCAGAAGAAAAAGGAAACCTGCCCTTCCCTCCTGATGTGCCAGTCCCATATACTGGTTCTGGGGCTGAGACTCCTAGTTATCAGGAAAGGAAAACACAACTAAGAGCACGTGATGGATCTCCTCATGTGGCTCCAAGTCAAAATACAAGAGACAAGAATTACCTTGAAAATCAGAAGGAAGAATCAATTGAAAGCGTGGATGGTAAACGCAGTCCAGGGACATCATCACCTGTCACAAATAGGGCTGCACGTGAGTCAAGTGCTGAGTATAGAGATAGTGACCAGGATGAGCCTGTGCTGGCTGATGGAAGCTCTGAAATGGGAAAGGAGGAAACGGCTACGGTTGCTGAAAATGATGCCCTGCAAGATGGGCCTCCGAAGCATAAAAAATTAGTTTCAAGAGTCGAACAATCTGCTGACGAAGAGCTTGATGATGGAGAGGACTTGAAGGCTGCAAGAAGTAGTGACAACAGCAAAGCAAGATCAGGAAGCAGCAGGGATTATCAGAAGTGGAGGGATGGGGTCGAAGAGGAAGTCATTCAAGGACGTTCAATGCATATGGGGGGCATCAAGAGGCATCTTGATGAGAATGAACAGGGGTTCCAGAGGAAAAACCGTGATGGTAGACAGGAACCAGATAGAAGTCACGTGGTAGTAAAAGGGAGGGAAGGCTCTTACCCTTATAGGGACTGGGATCCTAGCTCTGCACATCAGTTGCAATTGAAAAATGATGGCCTTCATAGGCGAAAGGAGAGGGAGAATCTTGATGGAGCATGGCAACAGAGAGAAGATGATCCTTATAGCAGATCGATCAGGCCTGAAGAAACAAGGAAGAGAGAACATGGTGATGAAATGGGATCCAGGCACAGGAGTAAGGGTCGAGAAAGTGACAGGAGTGACAAAGACGAACATCTGCAGTCAAGAAAACAGTTGGACAATGGCAGCTATAGGGCTTATCATGATAAGGATGTTGGTTCACGACCCAGGGAAAGAGAGGGTAGTTTGAAGGGCAGGTATGAACACGTGGAAGATTACCATGGCAAGAGAAGGAAAGATGAGGAATATATAAAGAGAGACCATATAGATAAAGAAGATTTTTTGCATGGCCACAGAGATAATACCACTCGCCGAAAGCGTGAAAGGGATGAAATTCTGGATCCGCGTAAGAGAGACGAGCAGCAGAGAGTTAGAGAAAATCTTGATGATCTGCACTCTGTTAGGCACAAAGATGACAGCTGGTCGCAAAGGGAGAGAGGTGACAGGCAAAGAGAGAGGGAGGAGTGGCCTAGGGTCAAACAATCCCATGAGGAAAATATACCCAAGCGCGAAAGAGATGAAGGACGAGTTGCTATAAGGGGTGGCCGTGGTGCAGAAGACAAAGCATGGGTTGGCCATACTAGGGCAAAGGAGGAGAACAAAGGCTCTGATAAGGAGCACCAATATAAAGAGACTGTGCGGCACAGTGAACCATCTAAAAGGAGAGATAGGGTTGAGGAAGAAAGCTCGCATCATAGGGGACGTGACGATGTTCATGGACGTGGAAATCAAATAACTAATGACCAGAAAAGATCTGGAAAGGAGAGATCAAGTACTCGTAATGAGCATGCTGACAACCAAAAGGTCCATGACAGAAAACATAAAGAAAGTTCAAGGAAGACTAAAGAATCTGTGATTGCTAATAACAGCACCACTTCCAAGAGACGTCAAGAAGATCAAAGTGGTCAGAATAAGGAGATGGGCTTGAAAGGCACCCATGTGCAAGGAACCGGTGAGGAAATCCCACAGCAGCGTCACTCTTCCAAAAGGCATAAAGAAGATGCGTCCTCTGATGATGAACAACGGGATTCCAAAAAAGGGCGCTCCAAATTAGAACGCTGGACAAGCCATAAGGAGAGGGATTTTGGTATCAGCAATAAATCATCGTTGAAGCTCAAAGAGCTTGATAGGATCAACAACAGAGGATCCTCAGATAACAGTAGAGTTCCAGAGGAATCTTCCAAGCCAGTTGAGGCTGTTGATACTCAACAATCAATGGCCGAGGAGAAGGATGCCAGTGATCAGGATATCAAGGACTCAGACACAAAACCACTTGATGCTGATGCAAAACCATTGGAGGAGCGACACCTGGACACAGTCGAGAAGTTGAAGAAAAGAAGTGAGCGTTTCAAGCTTCCGATGCCAAGTGAAAAAGAACCAGTGGCAATAAAAAAGGTGGAGAATGATGTGCCACCTTCCACCAATAGCGAGACTCAAACTCAGACTCAGACTCCTGTGGAGTCGGAGATCAAACCAGAACGGCCAGCTAGGAAAAGAAGGTGGATCAGCAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003676 GO:0003723 GO:0005488 GO:0005515 GO:0042802 GO:0097159 GO:1901363
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Fip1

Protein Analysis

1364

Amino Acids

153.19

Weight (kDa)

5.36

Isoelectric Point (pI)

54.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Fip1 PF05182 375 - 417 3.3e-20 Fip1 motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1707
AatII GACGTC 1 cut(s) 3466
Acc36I ACCTGC 2 cut(s) 1788, 2763
AccB1I GGYRCC 1 cut(s) 3509
AccB7I CCANNNNNTGG 5 cut(s) 476, 697, 809, 884, 1814
AccBSI CCGCTC 1 cut(s) 275
AccI GTMKAC 2 cut(s) 1128, 2386
AccII CGCG 2 cut(s) 2923, 3087
AcoI YGGCCR 6 cut(s) 958, 2866, 3121, 3151, 3795, 4058
AcsI RAATTY 4 cut(s) 17, 388, 1275, 2910
AcuI CTGAAG 1 cut(s) 2601
AcvI CACGTG 4 cut(s) 1871, 2012, 2410, 2785
AcyI GRCGYC 1 cut(s) 3463
AfaI GTAC 6 cut(s) 815, 857, 954, 1039, 1682, 3352
AfeI AGCGCT 1 cut(s) 727
AflIII ACRYGT 1 cut(s) 2782
AgeI ACCGGT 1 cut(s) 3527
AhdI GACNNNNNGTC 1 cut(s) 2998
AjiI CACGTC 2 cut(s) 3281, 3299
AjuI GAANNNNNNNTTGG 2 cut(s) 1467, 1499
AleI CACNNNNGTG 1 cut(s) 3515
AloI GAACNNNNNNTCC 2 cut(s) 2346, 2378
Alw21I GWGCWC 2 cut(s) 1870, 3198
AlwNI CAGNNNCTG 1 cut(s) 4089
Ama87I CYCGRG 2 cut(s) 449, 1521
Aor51HI AGCGCT 1 cut(s) 727
ApoI RAATTY 4 cut(s) 17, 388, 1275, 2910
ArsI GACNNNNNNTTYG 3 cut(s) 34, 2280, 2312
AsiGI ACCGGT 1 cut(s) 3527
Asp700I GAANNNNTTC 2 cut(s) 1245, 2298
AspA2I CCTAGG 1 cut(s) 3047
AspLEI GCGC 5 cut(s) 88, 728, 872, 3087, 3621
AspS9I GGNCC 7 cut(s) 436, 602, 887, 902, 947, 2132, 3382
AsuC2I CCSGG 4 cut(s) 440, 450, 451, 769
AvaI CYCGRG 2 cut(s) 449, 1521
AvaII GGWCC 3 cut(s) 436, 947, 3382
AvrII CCTAGG 1 cut(s) 3047
BaeGI GKGCMC 1 cut(s) 801
BaeI ACNNNNGTAYC 2 cut(s) 805, 838
BalI TGGCCA 2 cut(s) 2868, 3153
BamHI GGATCC 4 cut(s) 2455, 2618, 2917, 3722
BanI GGYRCC 1 cut(s) 3509
BanII GRGCYC 1 cut(s) 663
BbrPI CACGTG 4 cut(s) 1871, 2012, 2410, 2785
BbsI GAAGAC 4 cut(s) 12, 779, 3141, 3422
Bbv12I GWGCWC 2 cut(s) 1870, 3198
BceAI ACGGC 3 cut(s) 2112, 3108, 4073
BcgI CGANNNNNNTGC 2 cut(s) 2156, 2190
BciVI GTATCC 2 cut(s) 454, 1610
BclI TGATCA 2 cut(s) 1621, 3817
BcnI CCSGG 4 cut(s) 440, 450, 451, 769
BfaI CTAG 8 cut(s) 230, 264, 291, 1836, 2460, 3048, 3159, 4065
BfmI CTRYAG 4 cut(s) 1010, 1587, 2675, 2707
BfoI RGCGCY 3 cut(s) 729, 873, 3622
BfuAI ACCTGC 2 cut(s) 1788, 2763
BfuI GTATCC 2 cut(s) 454, 1610
BglI GCCNNNNNGGC 1 cut(s) 278
BglII AGATCT 3 cut(s) 25, 1321, 3329
BlnI CCTAGG 1 cut(s) 3047
BmcAI AGTACT 2 cut(s) 1682, 3352
Bme18I GGWCC 3 cut(s) 436, 947, 3382
BmeRI GACNNNNNGTC 1 cut(s) 2998
BmeT110I CYCGRG 2 cut(s) 449, 1521
BmgBI CACGTC 2 cut(s) 3281, 3299
BmgT120I GGNCC 7 cut(s) 436, 602, 887, 902, 947, 2132, 3382
BmrI ACTGGG 1 cut(s) 2461
BmuI ACTGGG 1 cut(s) 2461
BpiI GAAGAC 4 cut(s) 12, 779, 3141, 3422
BpmI CTGGAG 3 cut(s) 927, 997, 1109
BpuMI CCSGG 4 cut(s) 440, 450, 451, 769
Bsa29I ATCGAT 2 cut(s) 138, 2570
BsaAI YACGTR 5 cut(s) 859, 1871, 2012, 2410, 2785
BsaBI GATNNNNATC 1 cut(s) 1320
BsaHI GRCGYC 1 cut(s) 3463
BsaI GGTCTC 2 cut(s) 973, 2829
BsaWI WCCGGW 1 cut(s) 3527
BsaXI ACNNNNNCTCC 8 cut(s) 89, 119, 217, 247, 2623, 2653, 4012, 4042
Bse118I RCCGGY 2 cut(s) 782, 3527
Bse8I GATNNNNATC 1 cut(s) 1320
BseCI ATCGAT 2 cut(s) 138, 2570
BseGI GGATG 8 cut(s) 388, 832, 1966, 2053, 2284, 2734, 2911, 3814
BseJI GATNNNNATC 1 cut(s) 1320
BseMII CTCAG 8 cut(s) 743, 860, 1818, 2016, 3741, 3849, 4031, 4037
BseRI GAGGAG 9 cut(s) 174, 416, 924, 996, 1872, 3053, 3185, 3815, 3890
BseSI GKGCMC 1 cut(s) 801
BseYI CCCAGC 1 cut(s) 1047
BsgI GTGCAG 4 cut(s) 1992, 2451, 2954, 3150
Bsh1236I CGCG 2 cut(s) 2923, 3087
Bsh1285I CGRYCG 1 cut(s) 138
BshNI GGYRCC 1 cut(s) 3509
BshTI ACCGGT 1 cut(s) 3527
BshVI ATCGAT 2 cut(s) 138, 2570
BsiEI CGRYCG 1 cut(s) 138
BsiHKAI GWGCWC 2 cut(s) 1870, 3198
BsiHKCI CYCGRG 2 cut(s) 449, 1521
BsiSI CCGG 6 cut(s) 278, 440, 450, 768, 783, 3528
BslFI GGGAC 8 cut(s) 46, 134, 560, 899, 1790, 1993, 2462, 3291
BsmBI CGTCTC 3 cut(s) 1499, 2925, 3454
BsmFI GGGAC 8 cut(s) 46, 134, 560, 899, 1790, 1993, 2462, 3291
Bso31I GGTCTC 2 cut(s) 973, 2829
BsoBI CYCGRG 2 cut(s) 449, 1521
Bsp1286I GDGCHC 4 cut(s) 663, 801, 1870, 3198
Bsp19I CCATGG 1 cut(s) 2797
BspCNI CTCAG 8 cut(s) 742, 859, 1819, 2017, 3740, 3848, 4030, 4036
BspDI ATCGAT 2 cut(s) 138, 2570
BspFNI CGCG 2 cut(s) 2923, 3087
BspHI TCATGA 2 cut(s) 1357, 2719
BspMAI CTGCAG 3 cut(s) 1014, 1591, 2679
BspMI ACCTGC 2 cut(s) 1788, 2763
BspQI GCTCTTC 2 cut(s) 1453, 2178
BspT107I GGYRCC 1 cut(s) 3509
BspTNI GGTCTC 2 cut(s) 973, 2829
BsrBI CCGCTC 1 cut(s) 275
BsrFI RCCGGY 2 cut(s) 782, 3527
BssAI RCCGGY 2 cut(s) 782, 3527
BssNI GRCGYC 1 cut(s) 3463
BssT1I CCWWGG 3 cut(s) 1154, 2797, 3047
Bst4CI ACNGT 8 cut(s) 131, 2104, 2378, 2691, 3214, 3224, 3737, 3895
Bst6I CTCTTC 8 cut(s) 153, 511, 714, 1453, 2178, 2283, 2588, 3562
BstACI GRCGYC 1 cut(s) 3463
BstBAI YACGTR 5 cut(s) 859, 1871, 2012, 2410, 2785
BstC8I GCNNGC 7 cut(s) 283, 784, 1338, 2061, 3266, 3366, 4062
BstDEI CTNAG 9 cut(s) 729, 846, 1827, 1862, 2025, 3727, 3835, 4017, 4023
BstDSI CCRYGG 4 cut(s) 336, 598, 2797, 3124
BstENI CCTNNNNNAGG 2 cut(s) 960, 1664
BstF5I GGATG 8 cut(s) 388, 832, 1966, 2053, 2284, 2734, 2911, 3814
BstFNI CGCG 2 cut(s) 2923, 3087
BstH2I RGCGCY 3 cut(s) 729, 873, 3622
BstHHI GCGC 5 cut(s) 88, 728, 872, 3087, 3621
BstMCI CGRYCG 1 cut(s) 138
BstNSI RCATGY 1 cut(s) 3368
BstSFI CTRYAG 4 cut(s) 1010, 1587, 2675, 2707
BstSLI GKGCMC 1 cut(s) 801
BstSNI TACGTA 1 cut(s) 859
BstUI CGCG 2 cut(s) 2923, 3087
BstV2I GAAGAC 4 cut(s) 12, 779, 3141, 3422
BstX2I RGATCY 9 cut(s) 25, 168, 1321, 1877, 2455, 2618, 2917, 3329, 3722
BstYI RGATCY 9 cut(s) 25, 168, 1321, 1877, 2455, 2618, 2917, 3329, 3722
Bsu15I ATCGAT 2 cut(s) 138, 2570
BsuI GTATCC 2 cut(s) 454, 1610
BsuTUI ATCGAT 2 cut(s) 138, 2570
BtgI CCRYGG 4 cut(s) 336, 598, 2797, 3124
BtrI CACGTC 2 cut(s) 3281, 3299
BtsCI GGATG 8 cut(s) 388, 832, 1966, 2053, 2284, 2734, 2911, 3814
BtsIMutI CAGTG 4 cut(s) 474, 3229, 3820, 3963
BveI ACCTGC 2 cut(s) 1788, 2763
Cac8I GCNNGC 7 cut(s) 283, 784, 1338, 2061, 3266, 3366, 4062
CaiI CAGNNNCTG 1 cut(s) 4089
CciI TCATGA 2 cut(s) 1357, 2719
CfoI GCGC 5 cut(s) 88, 728, 872, 3087, 3621
Cfr10I RCCGGY 2 cut(s) 782, 3527
Cfr13I GGNCC 7 cut(s) 436, 602, 887, 902, 947, 2132, 3382
Cfr9I CCCGGG 1 cut(s) 449
ClaI ATCGAT 2 cut(s) 138, 2570
CseI GACGC 4 cut(s) 1427, 1481, 3539, 3570
Csp6I GTAC 6 cut(s) 814, 856, 953, 1038, 1681, 3351
CspAI ACCGGT 1 cut(s) 3527
CspCI CAANNNNNGTGG 2 cut(s) 1452, 1487
CviQI GTAC 6 cut(s) 814, 856, 953, 1038, 1681, 3351
DdeI CTNAG 9 cut(s) 729, 846, 1827, 1862, 2025, 3727, 3835, 4017, 4023
DraI TTTAAA 1 cut(s) 939
DriI GACNNNNNGTC 1 cut(s) 2998
EaeI YGGCCR 6 cut(s) 958, 2866, 3121, 3151, 3795, 4058
Eam1104I CTCTTC 8 cut(s) 153, 511, 714, 1453, 2178, 2283, 2588, 3562
Eam1105I GACNNNNNGTC 1 cut(s) 2998
EarI CTCTTC 8 cut(s) 153, 511, 714, 1453, 2178, 2283, 2588, 3562
EciI GGCGGA 1 cut(s) 97
Eco105I TACGTA 1 cut(s) 859
Eco130I CCWWGG 3 cut(s) 1154, 2797, 3047
Eco147I AGGCCT 1 cut(s) 2578
Eco24I GRGCYC 1 cut(s) 663
Eco31I GGTCTC 2 cut(s) 973, 2829
Eco32I GATATC 1 cut(s) 3826
Eco47I GGWCC 3 cut(s) 436, 947, 3382
Eco47III AGCGCT 1 cut(s) 727
Eco57I CTGAAG 1 cut(s) 2601
Eco72I CACGTG 4 cut(s) 1871, 2012, 2410, 2785
Eco88I CYCGRG 2 cut(s) 449, 1521
EcoNI CCTNNNNNAGG 2 cut(s) 960, 1664
EcoO109I RGGNCCY 1 cut(s) 947
EcoRI GAATTC 1 cut(s) 17
EcoRV GATATC 1 cut(s) 3826
EcoT14I CCWWGG 3 cut(s) 1154, 2797, 3047
EcoT22I ATGCAT 1 cut(s) 2319
EcoT38I GRGCYC 1 cut(s) 663
ErhI CCWWGG 3 cut(s) 1154, 2797, 3047
Esp3I CGTCTC 3 cut(s) 1499, 2925, 3454
FalI AAGNNNNNCTT 2 cut(s) 1644, 1676
FaqI GGGAC 8 cut(s) 46, 134, 560, 899, 1790, 1993, 2462, 3291
FauI CCCGC 2 cut(s) 244, 278
FauNDI CATATG 1 cut(s) 2319
FbaI TGATCA 2 cut(s) 1621, 3817
FblI GTMKAC 2 cut(s) 1128, 2386
FokI GGATG 8 cut(s) 395, 819, 1973, 2060, 2291, 2741, 2918, 3821
FriOI GRGCYC 1 cut(s) 663
FspBI CTAG 8 cut(s) 230, 264, 291, 1836, 2460, 3048, 3159, 4065
GlaI GCGC 5 cut(s) 87, 727, 871, 3086, 3620
GsaI CCCAGC 1 cut(s) 1051
GsuI CTGGAG 3 cut(s) 927, 997, 1109
HaeII RGCGCY 3 cut(s) 729, 873, 3622
HapII CCGG 6 cut(s) 278, 440, 450, 768, 783, 3528
HgaI GACGC 4 cut(s) 1427, 1481, 3539, 3570
HhaI GCGC 5 cut(s) 88, 728, 872, 3087, 3621
Hin1I GRCGYC 1 cut(s) 3463
Hin6I GCGC 5 cut(s) 86, 726, 870, 3085, 3619
HinP1I GCGC 5 cut(s) 86, 726, 870, 3085, 3619
HincII GTYRAC 1 cut(s) 1129
HindII GTYRAC 1 cut(s) 1129
HindIII AAGCTT 1 cut(s) 3929
HpaII CCGG 6 cut(s) 278, 440, 450, 768, 783, 3528
Hpy99I CGWCG 6 cut(s) 14, 17, 158, 557, 566, 569
HpyCH4III ACNGT 8 cut(s) 131, 2104, 2378, 2691, 3214, 3224, 3737, 3895
HpyF3I CTNAG 9 cut(s) 729, 846, 1827, 1862, 2025, 3727, 3835, 4017, 4023
Hsp92I GRCGYC 1 cut(s) 3463
HspAI GCGC 5 cut(s) 86, 726, 870, 3085, 3619
KroI GCCGGC 1 cut(s) 782
KroNI GCCGGC 1 cut(s) 784
Ksp22I TGATCA 2 cut(s) 1621, 3817
LguI GCTCTTC 2 cut(s) 1453, 2178
LmnI GCTCC 9 cut(s) 187, 238, 280, 416, 1897, 2531, 3193, 3626, 3877
MaeI CTAG 8 cut(s) 230, 264, 291, 1836, 2460, 3048, 3159, 4065
MbiI CCGCTC 1 cut(s) 275
MfeI CAATTG 2 cut(s) 1947, 2480
MflI RGATCY 9 cut(s) 25, 168, 1321, 1877, 2455, 2618, 2917, 3329, 3722
MhlI GDGCHC 4 cut(s) 663, 801, 1870, 3198
MlsI TGGCCA 2 cut(s) 2868, 3153
MluNI TGGCCA 2 cut(s) 2868, 3153
MmeI TCCRAC 3 cut(s) 1475, 2673, 4020
Mox20I TGGCCA 2 cut(s) 2868, 3153
Mph1103I ATGCAT 1 cut(s) 2319
MroNI GCCGGC 1 cut(s) 782
MroXI GAANNNNTTC 2 cut(s) 1245, 2298
MscI TGGCCA 2 cut(s) 2868, 3153
MseI TTAA 3 cut(s) 852, 938, 1202
MslI CAYNNNNRTG 5 cut(s) 2343, 2607, 2988, 3297, 3515
Msp20I TGGCCA 2 cut(s) 2868, 3153
MspA1I CMGCKG 6 cut(s) 91, 737, 779, 1237, 1343, 2997
MspI CCGG 6 cut(s) 278, 440, 450, 768, 783, 3528
MunI CAATTG 2 cut(s) 1947, 2480
MvnI CGCG 2 cut(s) 2923, 3087
NaeI GCCGGC 1 cut(s) 784
NciI CCSGG 4 cut(s) 440, 450, 451, 769
NcoI CCATGG 1 cut(s) 2797
NdeI CATATG 1 cut(s) 2319
NgoMIV GCCGGC 1 cut(s) 782
NmeAIII GCCGAG 2 cut(s) 986, 3823
NsiI ATGCAT 1 cut(s) 2319
NspI RCATGY 1 cut(s) 3368
OliI CACNNNNGTG 1 cut(s) 3515
PaeI GCATGC 1 cut(s) 3368
PagI TCATGA 2 cut(s) 1357, 2719
PasI CCCWGGG 2 cut(s) 1385, 2745
PceI AGGCCT 1 cut(s) 2578
PciSI GCTCTTC 2 cut(s) 1453, 2178
PcsI WCGNNNNNNNCGW 3 cut(s) 162, 561, 2893
PdiI GCCGGC 1 cut(s) 784
PdmI GAANNNNTTC 2 cut(s) 1245, 2298
PfeI GAWTC 9 cut(s) 56, 445, 1532, 1580, 1943, 2521, 3425, 3605, 3752
PflFI GACNNNGTC 1 cut(s) 3893
PflMI CCANNNNNTGG 5 cut(s) 476, 697, 809, 884, 1814
PinAI ACCGGT 1 cut(s) 3527
Ple19I CGATCG 1 cut(s) 138
PmaCI CACGTG 4 cut(s) 1871, 2012, 2410, 2785
PmlI CACGTG 4 cut(s) 1871, 2012, 2410, 2785
Ppu21I YACGTR 5 cut(s) 859, 1871, 2012, 2410, 2785
PpuMI RGGWCCY 1 cut(s) 947
PsiI TTATAA 1 cut(s) 1707
Psp5II RGGWCCY 1 cut(s) 947
PspCI CACGTG 4 cut(s) 1871, 2012, 2410, 2785
PspFI CCCAGC 1 cut(s) 1047
PspPI GGNCC 7 cut(s) 436, 602, 887, 902, 947, 2132, 3382
PspPPI RGGWCCY 1 cut(s) 947
PstI CTGCAG 3 cut(s) 1014, 1591, 2679
PstNI CAGNNNCTG 1 cut(s) 4089
PsuI RGATCY 9 cut(s) 25, 168, 1321, 1877, 2455, 2618, 2917, 3329, 3722
PsyI GACNNNGTC 1 cut(s) 3893
PvuI CGATCG 1 cut(s) 138
PvuII CAGCTG 3 cut(s) 779, 1237, 2997
RsaI GTAC 6 cut(s) 815, 857, 954, 1039, 1682, 3352
RsaNI GTAC 6 cut(s) 814, 856, 953, 1038, 1681, 3351
RseI CAYNNNNRTG 5 cut(s) 2343, 2607, 2988, 3297, 3515
SalI GTCGAC 1 cut(s) 1127
SapI GCTCTTC 2 cut(s) 1453, 2178
SaqAI TTAA 3 cut(s) 852, 938, 1202
Sau96I GGNCC 7 cut(s) 436, 602, 887, 902, 947, 2132, 3382
ScaI AGTACT 2 cut(s) 1682, 3352
SduI GDGCHC 4 cut(s) 663, 801, 1870, 3198
SfcI CTRYAG 4 cut(s) 1010, 1587, 2675, 2707
SinI GGWCC 3 cut(s) 436, 947, 3382
SmaI CCCGGG 1 cut(s) 451
SmiMI CAYNNNNRTG 5 cut(s) 2343, 2607, 2988, 3297, 3515
SnaBI TACGTA 1 cut(s) 859
SphI GCATGC 1 cut(s) 3368
SseBI AGGCCT 1 cut(s) 2578
SspI AATATT 1 cut(s) 349
SspMI CTAG 8 cut(s) 230, 264, 291, 1836, 2460, 3048, 3159, 4065
StuI AGGCCT 1 cut(s) 2578
StyI CCWWGG 3 cut(s) 1154, 2797, 3047
TaaI ACNGT 8 cut(s) 131, 2104, 2378, 2691, 3214, 3224, 3737, 3895
TatI WGTACW 2 cut(s) 1680, 3350
TauI GCSGC 8 cut(s) 91, 113, 242, 275, 636, 789, 1346, 3220
TfiI GAWTC 9 cut(s) 56, 445, 1532, 1580, 1943, 2521, 3425, 3605, 3752
Tru1I TTAA 3 cut(s) 852, 938, 1202
Tru9I TTAA 3 cut(s) 852, 938, 1202
TscAI CASTG 4 cut(s) 481, 3229, 3820, 3963
TspGWI ACGGA 2 cut(s) 325, 1623
TspMI CCCGGG 1 cut(s) 449
TspRI CASTG 4 cut(s) 481, 3229, 3820, 3963
Tth111I GACNNNGTC 1 cut(s) 3893
Van91I CCANNNNNTGG 5 cut(s) 476, 697, 809, 884, 1814
VpaK11BI GGWCC 3 cut(s) 436, 947, 3382
XagI CCTNNNNNAGG 2 cut(s) 960, 1664
XapI RAATTY 4 cut(s) 17, 388, 1275, 2910
XceI RCATGY 1 cut(s) 3368
XcmI CCANNNNNNNNNTGG 1 cut(s) 3329
XmaI CCCGGG 1 cut(s) 449
XmaJI CCTAGG 1 cut(s) 3047
XmiI GTMKAC 2 cut(s) 1128, 2386
XmnI GAANNNNTTC 2 cut(s) 1245, 2298
XspI CTAG 8 cut(s) 230, 264, 291, 1836, 2460, 3048, 3159, 4065
ZraI GACGTC 1 cut(s) 3464
ZrmI AGTACT 2 cut(s) 1682, 3352
Zsp2I ATGCAT 1 cut(s) 2319
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.