pycom15g14450

phosphatase 2C

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0012553
Physical Location & Seq
Reverse (-)
9856643 .. 9859469
2827 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1464 bp
ATGTTTGGAATTCAAATCTTCCTCCGCCGACAATTCGACATATTTCCAACCACGCTTTCCCACAGAAGTCTGAATTCCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCAGAGACTACAACAACTACTACTACCATAACCAGAGGCCCCAATTTCCCACTCACTCACGTTCTCTCAGAATCTTCTTACCGGAGTCATCCAAAAATCCTATATTTTTCTTCCCTCTCTGTTTCTTACTACTTTCCGATTCTCTCAGCAACTCCGCAGCACCGCCATTAACGTTTTCATCAGAAGGAGCAAGAAGCATGTGCCTGATAGACGCAGAACAGCTCGGTCAAGAAATGGGCAACATGGAGAATAACAATAACATCGATTCCAACGACAATGACATCGAGCAGTGGTCTCTGCAAGTCGACCGGACGGAGGAGAATTTGGACCGGGAAACTCCTGCTGTGGTGAGCTCAAGTACCCAGAATATGCTCAATTCGTTTCAACTTGAAAGCATAGGTGAGGATACAGTGGTTGCAGACAGAAAGCAGAGCCTGTTCAACTTTGTTCCTGCCCTTAGGTCAGGAGAGTGGTCTGATATTGGGGGTCGAACCCATATGGAGGATACTCATTTATGCATTGGTGACTTAGCTAAGAAGTTTGGTTATAATCCACTCAGCGAGGAAGCTATCTCTTTCTATGGTGTGTTTGATGGTCACGGAGGAAAGGATGCAGCACACTTTGTTCGTGACAATTTACCAAGAGTCATTGTTGAGGATGCTGACTTTCCTTTAGAACTTGAAAAGGTGATAACAAGGTCATTTATGGAGACAGATGCTGCGTTTGCAAAGAAGTGTTCTCATGAGGCTTCCCTTGCTTCTGGCACAACTGCACTCACTGCAATGATATTTGGGAGGTCCTTGCTTGTAGCAAATGCCGGAGATTGCCGGGCTGTATTATCACGATGTGGAGTGGCTATAGAAATGTCAAAGGATCATAGGCCCTGTTGCACAAAAGAAAGAATGCGTATTGAGTCATTGGGTGGATATATTGATGATGGTTATCTAAATGGTCAGTTAGGTGTAACCCGTGCATTAGGTAATTGGCATCTTGAAGGAATGAAGGACGCGGGTGAAAGGGGCGGACCCTTGAGCGCTATACCAGAACTTAAACTGACGACACTGGGCAAAGACGATGAGTTTTTGATCATTGGCACTGATGGGATATGGGATGTGTTCACCAGCCAAAATGCTGTTGATTTCGCTCGAAGGAGACTGCAAGAGCACAATGATGTGAAGTTGTGCTGCAAGCAAATAGTAGAGGAGGCAATAAAGCGGGGAGCAACGGACAATTTGACGCTTGTTATGGTGAGTTTCCACTTGGAGCCACCCCCACATGTGGCGGTAGAGAGACCTAGATTCAGACGAAGCATTTCTGCCGAGGGACTTCAGAATCTCAAATTCTTCTTAGATGGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2C

Protein Analysis

488

Amino Acids

54.4

Weight (kDa)

5.28

Isoelectric Point (pI)

46.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 198 - 446 1.4e-65 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 654
AccI GTMKAC 1 cut(s) 411
AccII CGCG 1 cut(s) 1115
AciI CCGC 7 cut(s) 25, 261, 269, 1115, 1128, 1321, 1388
AclI AACGTT 1 cut(s) 278
AclWI GGATC 1 cut(s) 987
AcsI RAATTY 4 cut(s) 9, 73, 427, 1445
AcuI CTGAAG 1 cut(s) 1418
AdeI CACNNNGTG 1 cut(s) 953
AfaI GTAC 1 cut(s) 466
AfeI AGCGCT 1 cut(s) 1141
AfiI CCNNNNNNNGG 3 cut(s) 421, 607, 1384
AflIII ACRYGT 1 cut(s) 1381
AgsI TTSAA 6 cut(s) 14, 491, 497, 547, 788, 1100
AjuI GAANNNNNNNTTGG 2 cut(s) 40, 72
AluBI AGCT 4 cut(s) 328, 459, 638, 674
AluI AGCT 4 cut(s) 328, 459, 638, 674
Alw21I GWGCWC 2 cut(s) 461, 1272
Alw26I GTCTC 5 cut(s) 105, 405, 809, 1252, 1390
AlwI GGATC 1 cut(s) 987
AlwNI CAGNNNCTG 2 cut(s) 541, 824
Aor51HI AGCGCT 1 cut(s) 1141
AoxI GGCC 2 cut(s) 143, 986
ApeKI GCWGC 4 cut(s) 263, 719, 824, 1290
ApoI RAATTY 4 cut(s) 9, 73, 427, 1445
Asp700I GAANNNNTTC 1 cut(s) 1417
AspLEI GCGC 1 cut(s) 1142
AspS9I GGNCC 5 cut(s) 144, 433, 903, 987, 1130
AsuC2I CCSGG 2 cut(s) 437, 935
AsuHPI GGTGA 7 cut(s) 466, 518, 641, 805, 1130, 1216, 1366
AvaII GGWCC 3 cut(s) 433, 903, 1130
AxyI CCTNAGG 1 cut(s) 563
BaeI ACNNNNGTAYC 2 cut(s) 504, 537
BanII GRGCYC 1 cut(s) 461
Bbv12I GWGCWC 2 cut(s) 461, 1272
BbvI GCAGC 4 cut(s) 275, 731, 811, 1277
BccI CCATC 4 cut(s) 692, 1037, 1199, 1451
BciVI GTATCC 2 cut(s) 505, 604
BclI TGATCA 1 cut(s) 1191
BcnI CCSGG 2 cut(s) 437, 935
BcoDI GTCTC 5 cut(s) 105, 405, 809, 1252, 1390
BfaI CTAG 1 cut(s) 1401
BfmI CTRYAG 1 cut(s) 963
BfoI RGCGCY 1 cut(s) 1143
BfuI GTATCC 2 cut(s) 505, 604
BisI GCNGC 4 cut(s) 264, 720, 825, 1291
BlsI GCNGC 4 cut(s) 265, 721, 826, 1292
Bme1390I CCNGG 2 cut(s) 437, 935
Bme18I GGWCC 3 cut(s) 433, 903, 1130
BmgT120I GGNCC 5 cut(s) 144, 433, 903, 987, 1130
BmiI GGNNCC 3 cut(s) 146, 1132, 1371
BmrFI CCNGG 2 cut(s) 437, 935
BmrI ACTGGG 1 cut(s) 1178
BmsI GCATC 4 cut(s) 706, 754, 811, 1102
BmuI ACTGGG 1 cut(s) 1178
BpuEI CTTGAG 2 cut(s) 445, 1156
BpuMI CCSGG 2 cut(s) 437, 935
Bsa29I ATCGAT 1 cut(s) 369
BsaBI GATNNNNATC 1 cut(s) 1047
BsaI GGTCTC 2 cut(s) 405, 1390
BsaJI CCNNGG 1 cut(s) 1425
BsaWI WCCGGW 2 cut(s) 187, 414
Bsc4I CCNNNNNNNGG 3 cut(s) 421, 607, 1384
Bse1I ACTGG 1 cut(s) 1173
Bse21I CCTNAGG 1 cut(s) 563
Bse3DI GCAATG 1 cut(s) 894
Bse8I GATNNNNATC 1 cut(s) 1047
BseCI ATCGAT 1 cut(s) 369
BseDI CCNNGG 1 cut(s) 1425
BseGI GGATG 4 cut(s) 194, 721, 769, 1222
BseJI GATNNNNATC 1 cut(s) 1047
BseLI CCNNNNNNNGG 3 cut(s) 421, 607, 1384
BseMI GCAATG 1 cut(s) 894
BseMII CTCAG 4 cut(s) 121, 187, 265, 676
BseNI ACTGG 1 cut(s) 1173
BseRI GAGGAG 2 cut(s) 437, 1322
BseXI GCAGC 4 cut(s) 275, 731, 811, 1277
BsgI GTGCAG 1 cut(s) 861
Bsh1236I CGCG 1 cut(s) 1115
Bsh1285I CGRYCG 1 cut(s) 415
BshFI GGCC 2 cut(s) 145, 988
BshVI ATCGAT 1 cut(s) 369
BsiEI CGRYCG 1 cut(s) 415
BsiHKAI GWGCWC 2 cut(s) 461, 1272
BsiSI CCGG 5 cut(s) 188, 415, 436, 924, 934
BslFI GGGAC 1 cut(s) 1443
BslI CCNNNNNNNGG 3 cut(s) 421, 607, 1384
BsmAI GTCTC 5 cut(s) 105, 405, 809, 1252, 1390
BsmFI GGGAC 1 cut(s) 1443
BsmI GAATGC 1 cut(s) 1014
BsnI GGCC 2 cut(s) 145, 988
Bso31I GGTCTC 2 cut(s) 405, 1390
Bsp1286I GDGCHC 2 cut(s) 461, 1272
Bsp143I GATC 2 cut(s) 979, 1191
BspACI CCGC 7 cut(s) 25, 261, 269, 1115, 1128, 1321, 1388
BspANI GGCC 2 cut(s) 145, 988
BspCNI CTCAG 4 cut(s) 120, 186, 264, 675
BspDI ATCGAT 1 cut(s) 369
BspFNI CGCG 1 cut(s) 1115
BspHI TCATGA 1 cut(s) 847
BspLI GGNNCC 3 cut(s) 146, 1132, 1371
BspPI GGATC 1 cut(s) 987
BspTNI GGTCTC 2 cut(s) 405, 1390
BsrDI GCAATG 1 cut(s) 894
BsrI ACTGG 1 cut(s) 1173
BssECI CCNNGG 1 cut(s) 1425
BssMI GATC 2 cut(s) 979, 1191
Bst4CI ACNGT 1 cut(s) 517
BstAPI GCANNNNNTGC 1 cut(s) 884
BstC8I GCNNGC 1 cut(s) 1295
BstDEI CTNAG 8 cut(s) 107, 173, 251, 563, 634, 639, 662, 1453
BstF5I GGATG 4 cut(s) 194, 721, 769, 1222
BstFNI CGCG 1 cut(s) 1115
BstH2I RGCGCY 1 cut(s) 1143
BstHHI GCGC 1 cut(s) 1142
BstKTI GATC 2 cut(s) 982, 1194
BstMAI GTCTC 5 cut(s) 105, 405, 809, 1252, 1390
BstMBI GATC 2 cut(s) 979, 1191
BstMCI CGRYCG 1 cut(s) 415
BstMWI GCNNNNNNNGC 3 cut(s) 830, 860, 884
BstNSI RCATGY 2 cut(s) 307, 1385
BstSCI CCNGG 2 cut(s) 435, 933
BstSFI CTRYAG 1 cut(s) 963
BstUI CGCG 1 cut(s) 1115
BstV1I GCAGC 4 cut(s) 275, 731, 811, 1277
Bsu15I ATCGAT 1 cut(s) 369
Bsu36I CCTNAGG 1 cut(s) 563
BsuI GTATCC 2 cut(s) 505, 604
BsuRI GGCC 2 cut(s) 145, 988
BsuTUI ATCGAT 1 cut(s) 369
BtsCI GGATG 4 cut(s) 194, 721, 769, 1222
BtsI GCAGTG 2 cut(s) 401, 882
BtsIMutI CAGTG 5 cut(s) 401, 522, 882, 1166, 1200
Cac8I GCNNGC 1 cut(s) 1295
CaiI CAGNNNCTG 2 cut(s) 541, 824
CciI TCATGA 1 cut(s) 847
CfoI GCGC 1 cut(s) 1142
Cfr13I GGNCC 5 cut(s) 144, 433, 903, 987, 1130
ClaI ATCGAT 1 cut(s) 369
CseI GACGC 3 cut(s) 326, 1121, 1351
Csp6I GTAC 1 cut(s) 465
CviAII CATG 4 cut(s) 304, 349, 848, 1382
CviQI GTAC 1 cut(s) 465
DdeI CTNAG 8 cut(s) 107, 173, 251, 563, 634, 639, 662, 1453
DpnI GATC 2 cut(s) 981, 1193
DpnII GATC 2 cut(s) 979, 1191
DraIII CACNNNGTG 1 cut(s) 953
EciI GGCGGA 2 cut(s) 14, 1143
Ecl136II GAGCTC 1 cut(s) 459
Eco24I GRGCYC 1 cut(s) 461
Eco31I GGTCTC 2 cut(s) 405, 1390
Eco47I GGWCC 3 cut(s) 433, 903, 1130
Eco47III AGCGCT 1 cut(s) 1141
Eco53kI GAGCTC 1 cut(s) 459
Eco57I CTGAAG 1 cut(s) 1418
Eco81I CCTNAGG 1 cut(s) 563
EcoICRI GAGCTC 1 cut(s) 459
EcoO109I RGGNCCY 3 cut(s) 144, 903, 987
EcoRI GAATTC 2 cut(s) 9, 73
EcoT22I ATGCAT 1 cut(s) 626
EcoT38I GRGCYC 1 cut(s) 461
FaeI CATG 4 cut(s) 307, 352, 851, 1385
FaqI GGGAC 1 cut(s) 1443
FatI CATG 4 cut(s) 303, 348, 847, 1381
FauI CCCGC 2 cut(s) 1108, 1314
FauNDI CATATG 1 cut(s) 603
FbaI TGATCA 1 cut(s) 1191
FblI GTMKAC 1 cut(s) 411
Fnu4HI GCNGC 4 cut(s) 264, 720, 825, 1291
FokI GGATG 4 cut(s) 181, 728, 776, 1229
FriOI GRGCYC 1 cut(s) 461
Fsp4HI GCNGC 4 cut(s) 264, 720, 825, 1291
FspBI CTAG 1 cut(s) 1401
GlaI GCGC 1 cut(s) 1141
GluI GCNGC 4 cut(s) 264, 720, 825, 1291
HaeII RGCGCY 1 cut(s) 1143
HaeIII GGCC 2 cut(s) 145, 988
HapII CCGG 5 cut(s) 188, 415, 436, 924, 934
HgaI GACGC 3 cut(s) 326, 1121, 1351
HhaI GCGC 1 cut(s) 1142
Hin1II CATG 4 cut(s) 307, 352, 851, 1385
Hin6I GCGC 1 cut(s) 1140
HinP1I GCGC 1 cut(s) 1140
HincII GTYRAC 1 cut(s) 412
HindII GTYRAC 1 cut(s) 412
HinfI GANTC 8 cut(s) 177, 191, 245, 371, 750, 1019, 1404, 1438
HpaII CCGG 5 cut(s) 188, 415, 436, 924, 934
HphI GGTGA 7 cut(s) 466, 518, 641, 805, 1130, 1216, 1366
Hpy166II GTNNAC 2 cut(s) 412, 1224
Hpy188I TCNGA 8 cut(s) 72, 110, 176, 244, 289, 583, 1409, 1437
Hpy188III TCNNGA 6 cut(s) 335, 570, 734, 848, 948, 1097
Hpy8I GTNNAC 2 cut(s) 412, 1224
HpyAV CCTTC 4 cut(s) 284, 1094, 1102, 1248
HpyCH4III ACNGT 1 cut(s) 517
HpyCH4IV ACGT 2 cut(s) 166, 278
HpyF10VI GCNNNNNNNGC 3 cut(s) 830, 860, 884
HpyF3I CTNAG 8 cut(s) 107, 173, 251, 563, 634, 639, 662, 1453
HpySE526I ACGT 2 cut(s) 166, 278
Hsp92II CATG 4 cut(s) 307, 352, 851, 1385
HspAI GCGC 1 cut(s) 1140
Ksp22I TGATCA 1 cut(s) 1191
Kzo9I GATC 2 cut(s) 979, 1191
LmnI GCTCC 3 cut(s) 293, 1325, 1369
Lsp1109I GCAGC 4 cut(s) 275, 731, 811, 1277
LweI GCATC 4 cut(s) 706, 754, 811, 1102
MaeI CTAG 1 cut(s) 1401
MaeII ACGT 2 cut(s) 166, 278
MaeIII GTNAC 4 cut(s) 629, 701, 734, 1069
MalI GATC 2 cut(s) 981, 1193
MboI GATC 2 cut(s) 979, 1191
MboII GAAGA 4 cut(s) 10, 172, 208, 1441
MhlI GDGCHC 2 cut(s) 461, 1272
MlyI GAGTC 3 cut(s) 200, 759, 1028
MmeI TCCRAC 2 cut(s) 71, 399
Mph1103I ATGCAT 1 cut(s) 626
MroXI GAANNNNTTC 1 cut(s) 1417
MseI TTAA 2 cut(s) 275, 1155
MslI CAYNNNNRTG 2 cut(s) 887, 1275
MspI CCGG 5 cut(s) 188, 415, 436, 924, 934
MspR9I CCNGG 2 cut(s) 437, 935
Mva1269I GAATGC 1 cut(s) 1014
MvnI CGCG 1 cut(s) 1115
MwoI GCNNNNNNNGC 3 cut(s) 830, 860, 884
NciI CCSGG 2 cut(s) 437, 935
NdeI CATATG 1 cut(s) 603
NdeII GATC 2 cut(s) 979, 1191
NlaIII CATG 4 cut(s) 307, 352, 851, 1385
NlaIV GGNNCC 3 cut(s) 146, 1132, 1371
NmeAIII GCCGAG 1 cut(s) 1450
NmuCI GTSAC 3 cut(s) 629, 701, 734
NsiI ATGCAT 1 cut(s) 626
NspI RCATGY 2 cut(s) 307, 1385
PagI TCATGA 1 cut(s) 847
PciI ACATGT 1 cut(s) 1381
PcsI WCGNNNNNNNCGW 1 cut(s) 375
PctI GAATGC 1 cut(s) 1014
PdmI GAANNNNTTC 1 cut(s) 1417
PfeI GAWTC 5 cut(s) 177, 245, 371, 1404, 1438
PkrI GCNGC 4 cut(s) 265, 721, 826, 1292
PleI GAGTC 3 cut(s) 199, 758, 1027
PpsI GAGTC 3 cut(s) 199, 758, 1027
PpuMI RGGWCCY 1 cut(s) 903
PscI ACATGT 1 cut(s) 1381
PsiI TTATAA 1 cut(s) 654
Psp124BI GAGCTC 1 cut(s) 461
Psp1406I AACGTT 1 cut(s) 278
Psp5II RGGWCCY 1 cut(s) 903
PspN4I GGNNCC 3 cut(s) 146, 1132, 1371
PspPI GGNCC 5 cut(s) 144, 433, 903, 987, 1130
PspPPI RGGWCCY 1 cut(s) 903
PstNI CAGNNNCTG 2 cut(s) 541, 824
RsaI GTAC 1 cut(s) 466
RsaNI GTAC 1 cut(s) 465
RseI CAYNNNNRTG 2 cut(s) 887, 1275
SacI GAGCTC 1 cut(s) 461
SalI GTCGAC 1 cut(s) 410
SaqAI TTAA 2 cut(s) 275, 1155
SatI GCNGC 4 cut(s) 264, 720, 825, 1291
Sau3AI GATC 2 cut(s) 979, 1191
Sau96I GGNCC 5 cut(s) 144, 433, 903, 987, 1130
SchI GAGTC 3 cut(s) 200, 759, 1028
ScrFI CCNGG 2 cut(s) 437, 935
SduI GDGCHC 2 cut(s) 461, 1272
SfaNI GCATC 4 cut(s) 706, 754, 811, 1102
SfcI CTRYAG 1 cut(s) 963
SinI GGWCC 3 cut(s) 433, 903, 1130
SmiMI CAYNNNNRTG 2 cut(s) 887, 1275
SmlI CTYRAG 2 cut(s) 460, 1135
SmoI CTYRAG 2 cut(s) 460, 1135
SsiI CCGC 7 cut(s) 25, 261, 269, 1115, 1128, 1321, 1388
SspMI CTAG 1 cut(s) 1401
SstI GAGCTC 1 cut(s) 461
StyD4I CCNGG 2 cut(s) 435, 933
TaaI ACNGT 1 cut(s) 517
TaiI ACGT 2 cut(s) 169, 281
TaqI TCGA 6 cut(s) 36, 369, 390, 411, 595, 1252
TaqII GACCGA 1 cut(s) 320
TfiI GAWTC 5 cut(s) 177, 245, 371, 1404, 1438
Tru1I TTAA 2 cut(s) 275, 1155
Tru9I TTAA 2 cut(s) 275, 1155
TscAI CASTG 5 cut(s) 401, 522, 889, 1173, 1207
TseFI GTSAC 3 cut(s) 629, 701, 734
TseI GCWGC 4 cut(s) 263, 719, 824, 1290
Tsp45I GTSAC 3 cut(s) 629, 701, 734
TspDTI ATGAA 2 cut(s) 273, 1121
TspGWI ACGGA 3 cut(s) 434, 720, 1346
TspRI CASTG 5 cut(s) 401, 522, 889, 1173, 1207
VpaK11BI GGWCC 3 cut(s) 433, 903, 1130
XapI RAATTY 4 cut(s) 9, 73, 427, 1445
XceI RCATGY 2 cut(s) 307, 1385
XmiI GTMKAC 1 cut(s) 411
XmnI GAANNNNTTC 1 cut(s) 1417
XspI CTAG 1 cut(s) 1401
Zsp2I ATGCAT 1 cut(s) 626
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.