pycom15g15340

Protein of unknown function (DUF4050)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0009786
Physical Location & Seq
Reverse (-)
10683390 .. 10687072
3683 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 543 bp
ATGGCGGCGACGCTCAACACCTCCATGGCGGCCTGGATCCGCCACTTACTCGCTTGCATGGGTGGTTGTTTTGGATGCTGCACTAAACCCACACCAATTACTGCTGTCGATGAGCCTTCGAAGGGATTGAGAATTCAAGGGCGAAGTGTTAAGAAACCGGGCATCTCGGATGACTTTTGGAGCAGCAGTACTTATGATTTGGACAATAGTGCCGTTCAATCCCAGAGAAGCATCTCATCCATCAGCACATCCAACCAGACCCTCAACTTTGGCAGTGTGGCTGGTAGTAGCACAAGCAGCCAATCCGACTTCGTAAATAATGGTCTTGTTCTATGGAACCAGAACAGGCTTGAGTGGATCGGAAGTGGCAACCCTAGGAATCAAATTCCAAAAAGTCGGGAAACCAGAGTAAGTTGGAATGCAACTTATGAAAGATTGGTTGGAACCAAACAGAGACAGTCGCCTTATGCGCAACGCATTCCTTTGTCTGACATGGTAGAATTTCTCGTGGAAGTATGGGAGCAGGAGGGTCTGTACGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4050

Protein Analysis

181

Amino Acids

19.9

Weight (kDa)

7.65

Isoelectric Point (pI)

46.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
clamp_Gag1-like PF13259 63 - 180 1.5e-20 Gag1-like, clamp domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 471
AciI CCGC 3 cut(s) 5, 29, 40
AclWI GGATC 3 cut(s) 31, 44, 365
AcsI RAATTY 3 cut(s) 132, 384, 500
AfaI GTAC 2 cut(s) 190, 536
AfiI CCNNNNNNNGG 1 cut(s) 122
AgsI TTSAA 2 cut(s) 137, 218
AjnI CCWGG 1 cut(s) 32
AjuI GAANNNNNNNTTGG 2 cut(s) 423, 455
Alw26I GTCTC 1 cut(s) 448
AlwI GGATC 3 cut(s) 31, 44, 365
AoxI GGCC 1 cut(s) 30
ApeKI GCWGC 3 cut(s) 78, 183, 297
ApoI RAATTY 3 cut(s) 132, 384, 500
AspA2I CCTAGG 1 cut(s) 374
AspLEI GCGC 1 cut(s) 472
AsuC2I CCSGG 1 cut(s) 159
AsuII TTCGAA 1 cut(s) 119
AvrII CCTAGG 1 cut(s) 374
BamHI GGATCC 1 cut(s) 36
BauI CACGAG 1 cut(s) 506
BbvI GCAGC 3 cut(s) 65, 195, 309
BccI CCATC 1 cut(s) 248
BceAI ACGGC 1 cut(s) 197
BciT130I CCWGG 1 cut(s) 34
BcnI CCSGG 1 cut(s) 159
BcoDI GTCTC 1 cut(s) 448
BfaI CTAG 1 cut(s) 375
BisI GCNGC 5 cut(s) 6, 30, 79, 184, 298
BlnI CCTAGG 1 cut(s) 374
BlsI GCNGC 5 cut(s) 7, 31, 80, 185, 299
BmcAI AGTACT 1 cut(s) 190
Bme1390I CCNGG 2 cut(s) 34, 159
BmiI GGNNCC 3 cut(s) 38, 338, 445
BmrFI CCNGG 2 cut(s) 34, 159
BmsI GCATC 3 cut(s) 65, 171, 240
Bpu14I TTCGAA 1 cut(s) 119
BpuEI CTTGAG 1 cut(s) 371
BpuMI CCSGG 1 cut(s) 159
BsaJI CCNNGG 2 cut(s) 24, 374
BsaXI ACNNNNNCTCC 3 cut(s) 172, 202, 518
Bsc4I CCNNNNNNNGG 1 cut(s) 122
BseBI CCWGG 1 cut(s) 34
BseDI CCNNGG 2 cut(s) 24, 374
BseGI GGATG 4 cut(s) 80, 175, 236, 248
BseLI CCNNNNNNNGG 1 cut(s) 122
BseXI GCAGC 3 cut(s) 65, 195, 309
BsgI GTGCAG 1 cut(s) 64
BshFI GGCC 1 cut(s) 32
BsiSI CCGG 1 cut(s) 158
BslI CCNNNNNNNGG 1 cut(s) 122
BsmAI GTCTC 1 cut(s) 448
BsmI GAATGC 2 cut(s) 424, 477
BsnI GGCC 1 cut(s) 32
Bsp119I TTCGAA 1 cut(s) 119
Bsp143I GATC 2 cut(s) 36, 357
Bsp19I CCATGG 1 cut(s) 24
BspACI CCGC 3 cut(s) 5, 29, 40
BspANI GGCC 1 cut(s) 32
BspLI GGNNCC 3 cut(s) 38, 338, 445
BspPI GGATC 3 cut(s) 31, 44, 365
BspT104I TTCGAA 1 cut(s) 119
BssECI CCNNGG 2 cut(s) 24, 374
BssMI GATC 2 cut(s) 36, 357
BssSI CACGAG 1 cut(s) 506
BssT1I CCWWGG 2 cut(s) 24, 374
Bst2BI CACGAG 1 cut(s) 506
Bst2UI CCWGG 1 cut(s) 34
Bst4CI ACNGT 1 cut(s) 459
BstBI TTCGAA 1 cut(s) 119
BstC8I GCNNGC 1 cut(s) 55
BstDSI CCRYGG 1 cut(s) 24
BstF5I GGATG 4 cut(s) 80, 175, 236, 248
BstHHI GCGC 1 cut(s) 472
BstKTI GATC 2 cut(s) 39, 360
BstMAI GTCTC 1 cut(s) 448
BstMBI GATC 2 cut(s) 36, 357
BstMWI GCNNNNNNNGC 2 cut(s) 297, 469
BstNI CCWGG 1 cut(s) 34
BstSCI CCNGG 2 cut(s) 32, 157
BstV1I GCAGC 3 cut(s) 65, 195, 309
BstX2I RGATCY 1 cut(s) 36
BstYI RGATCY 1 cut(s) 36
BsuRI GGCC 1 cut(s) 32
BtgI CCRYGG 1 cut(s) 24
BtsCI GGATG 4 cut(s) 80, 175, 236, 248
BtsI GCAGTG 1 cut(s) 280
BtsIMutI CAGTG 1 cut(s) 280
Cac8I GCNNGC 1 cut(s) 55
CfoI GCGC 1 cut(s) 472
CseI GACGC 1 cut(s) 19
Csp6I GTAC 2 cut(s) 189, 535
CviAII CATG 3 cut(s) 25, 58, 493
CviJI RGCY 5 cut(s) 32, 115, 281, 300, 349
CviKI_1 RGCY 5 cut(s) 32, 115, 281, 300, 349
CviQI GTAC 2 cut(s) 189, 535
DpnI GATC 2 cut(s) 38, 359
DpnII GATC 2 cut(s) 36, 357
EciI GGCGGA 1 cut(s) 29
Eco130I CCWWGG 2 cut(s) 24, 374
EcoRI GAATTC 1 cut(s) 132
EcoRII CCWGG 1 cut(s) 32
EcoT14I CCWWGG 2 cut(s) 24, 374
ErhI CCWWGG 2 cut(s) 24, 374
FaeI CATG 3 cut(s) 28, 61, 496
FaiI YATR 8 cut(s) 26, 59, 195, 334, 429, 468, 494, 517
FatI CATG 3 cut(s) 24, 57, 492
Fnu4HI GCNGC 5 cut(s) 6, 30, 79, 184, 298
FokI GGATG 4 cut(s) 87, 182, 223, 235
Fsp4HI GCNGC 5 cut(s) 6, 30, 79, 184, 298
FspBI CTAG 1 cut(s) 375
FspI TGCGCA 1 cut(s) 471
GlaI GCGC 1 cut(s) 471
GluI GCNGC 5 cut(s) 6, 30, 79, 184, 298
HaeIII GGCC 1 cut(s) 32
HapII CCGG 1 cut(s) 158
HgaI GACGC 1 cut(s) 19
HhaI GCGC 1 cut(s) 472
Hin1II CATG 3 cut(s) 28, 61, 496
Hin6I GCGC 1 cut(s) 470
HinP1I GCGC 1 cut(s) 470
HinfI GANTC 1 cut(s) 379
HpaII CCGG 1 cut(s) 158
Hpy188I TCNGA 4 cut(s) 169, 307, 362, 490
Hpy188III TCNNGA 1 cut(s) 398
Hpy99I CGWCG 1 cut(s) 13
HpyAV CCTTC 2 cut(s) 115, 126
HpyCH4III ACNGT 1 cut(s) 459
HpyCH4V TGCA 3 cut(s) 57, 81, 422
HpyF10VI GCNNNNNNNGC 2 cut(s) 297, 469
Hsp92II CATG 3 cut(s) 28, 61, 496
HspAI GCGC 1 cut(s) 470
Kzo9I GATC 2 cut(s) 36, 357
LmnI GCTCC 2 cut(s) 180, 520
Lsp1109I GCAGC 3 cut(s) 65, 195, 309
LweI GCATC 3 cut(s) 65, 171, 240
MaeI CTAG 1 cut(s) 375
MalI GATC 2 cut(s) 38, 359
MboI GATC 2 cut(s) 36, 357
MflI RGATCY 1 cut(s) 36
MluCI AATT 4 cut(s) 96, 132, 384, 500
MmeI TCCRAC 4 cut(s) 276, 330, 395, 421
MnlI CCTC 3 cut(s) 31, 272, 520
MseI TTAA 1 cut(s) 150
MslI CAYNNNNRTG 1 cut(s) 23
MspI CCGG 1 cut(s) 158
MspR9I CCNGG 2 cut(s) 34, 159
Mva1269I GAATGC 2 cut(s) 424, 477
MvaI CCWGG 1 cut(s) 34
MwoI GCNNNNNNNGC 2 cut(s) 297, 469
NciI CCSGG 1 cut(s) 159
NcoI CCATGG 1 cut(s) 24
NdeII GATC 2 cut(s) 36, 357
NlaIII CATG 3 cut(s) 28, 61, 496
NlaIV GGNNCC 3 cut(s) 38, 338, 445
NsbI TGCGCA 1 cut(s) 471
NspV TTCGAA 1 cut(s) 119
PctI GAATGC 2 cut(s) 424, 477
PfeI GAWTC 1 cut(s) 379
PkrI GCNGC 5 cut(s) 7, 31, 80, 185, 299
Psp6I CCWGG 1 cut(s) 32
PspGI CCWGG 1 cut(s) 32
PspN4I GGNNCC 3 cut(s) 38, 338, 445
PsuI RGATCY 1 cut(s) 36
RsaI GTAC 2 cut(s) 190, 536
RsaNI GTAC 2 cut(s) 189, 535
RseI CAYNNNNRTG 1 cut(s) 23
SaqAI TTAA 1 cut(s) 150
SatI GCNGC 5 cut(s) 6, 30, 79, 184, 298
Sau3AI GATC 2 cut(s) 36, 357
ScaI AGTACT 1 cut(s) 190
ScrFI CCNGG 2 cut(s) 34, 159
SetI ASST 1 cut(s) 23
SfaNI GCATC 3 cut(s) 65, 171, 240
SfuI TTCGAA 1 cut(s) 119
SmiMI CAYNNNNRTG 1 cut(s) 23
SmlI CTYRAG 1 cut(s) 350
SmoI CTYRAG 1 cut(s) 350
Sse9I AATT 4 cut(s) 96, 132, 384, 500
SsiI CCGC 3 cut(s) 5, 29, 40
SspMI CTAG 1 cut(s) 375
StyD4I CCNGG 2 cut(s) 32, 157
StyI CCWWGG 2 cut(s) 24, 374
TaaI ACNGT 1 cut(s) 459
TaqI TCGA 2 cut(s) 108, 119
TasI AATT 4 cut(s) 96, 132, 384, 500
TatI WGTACW 1 cut(s) 188
TauI GCSGC 2 cut(s) 8, 32
TfiI GAWTC 1 cut(s) 379
Tru1I TTAA 1 cut(s) 150
Tru9I TTAA 1 cut(s) 150
TscAI CASTG 1 cut(s) 280
TseI GCWGC 3 cut(s) 78, 183, 297
TspDTI ATGAA 1 cut(s) 444
TspRI CASTG 1 cut(s) 280
XapI RAATTY 3 cut(s) 132, 384, 500
XmaJI CCTAGG 1 cut(s) 374
XspI CTAG 1 cut(s) 375
ZrmI AGTACT 1 cut(s) 190
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.