pycom15g16670

Belongs to the ubiquitin-conjugating enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0008037
Physical Location & Seq
Forward (+)
11705347 .. 11707993
2647 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1698 bp
ATGGCTCAAGCCGCGCGGCTTAACCTCCGACTGCAGAAGGAGCTCAAGCTCCTCCTCACTGATCCTCCGCCGGGCGCCTCTTTCCCTCTCCTCGCTTCCTCCTCCTCCCTCACCACCATCGACGCCCAGATCAAAGGTCCAGAAGGAACCGTGTACGCCGAAGGATTCTTCAACATTAAGATTCAGATACCCGAGAGGTACCCGTTTCAGCCTCCGAGTGTGACATTTGCGACGCCAATTTACCACCCGAATATCGACACCGGAGGCCGGATTTGCCTCGACATTCTCAATCTTCCTCCGAAGGGGGCATGGCAACCATCATTGAACATTTCAACTGTGCTTACAAGCATTGGGTTGTTATTGAGTGAACCCAATCCAGATGATGGCCTCATGTGTGAAGCGAGTAGGGAATTCAAGTACAACAGGCAAGCTTTCGACCAGAAAGCTCGATCTATGACTCAGAAATATGCCCAGGTGTCTGCTTCTGGGAGTGTTTGTGACTCTCAATCTCAACCTCAAAGCAGCGCAAATCCACTCACGGTGCTGCTGGAGGTTAAAGCTTCAATCCATGAGTCAAAACATGAAGCAGAGTCTGCTGTGAGTAGTCATAAGAAGGTAATTGGGGGTAGCAGGAAGCTGTCACTGGAGTCTTCAGGCCAAACCCAGAAAAGGAATGTTTGCGGGAAAGGAAATGAAGTGCCAAAAGTGCAACTCTTTGCCTCTGATTTCGGAAACAAGATGGAAGTCAAAGGAACAGAAAAGTTGCTGGAACATAAGTGCAGTCAGAGTCAGGAGAAGATATGTGGGAGCAGACAAAAATTGTCACTAGAAGCCCTCAGCCAACCTGAGAAGAAAAGTGATGGTATGGAGAATGTGGTGCCAAATCACCGTTCATCACTTAAACTGCAAAACCTTCCCGTGCCATCTTCAGGATCGGTCGTGCTGCAATCAGGAAACAGTTGCAAGCAAGGACTTCATCAGGGTCAAGATGGTAAATTGATAGATGACAGCACAGTCGTGAGATCAAAGAGGCTGCGTCTATCTGAAAACAAGCAGTCATTGGCTTCTTTAAACACATCCCAAATATTTGATGAAACCAACGATAAAAAGTTCATTACCCCTCAACTGCTGCCCTCAGTGTCCCATTCTGATGCGTTGTCTGAGCCTTTGTCAGCGGCACCAGCTCTTGACAGCATCTACTCACAACCACATAAAGATTTGATTGATGGAATGGGAAATGGCTCCGTTGGAATAAGTTGCAAGAAGCTTTGCTTAGCTGGCAAGAAGCTTTCGTTGGGATCCAGAGGCTCATCAGAGGCACATGGAAAGGCTAACAAGGAGAATGGGGCGCCAAAGGTGACTGAGAATCATACAAAATGTGAGATGGCTCATTTGCAAGAGAACAACAATAATAACATTCTTCTGCAGCCCCGGAGTCTCTCTTCATATCCATCCAAGGCCCTTCCTAAGCAACAGAATTTGAAGCGGGATGAGAAGCAACAATCTGATGAAGATTACAATAGTGGGATTGCTGCAGGGATTAATAAGCAAGAAGAAGGGAAAAAATTGGCAATGTGTGAGGCTGAGGCAGTGATTGTGCTGGATAGTGAAAGTAGTGATGAGGAAAGGAGTAGACCTGCGATGACTATATCAGTGCTAGCAAGAAAGCGCATGGGAAAGTGGAGAGTAAGAACTTGA

Protein Analysis

566

Amino Acids

61.45

Weight (kDa)

8.96

Isoelectric Point (pI)

58.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UQ_con PF00179 10 - 151 1.1e-40 Ubiquitin-conjugating enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1013
Acc36I ACCTGC 1 cut(s) 1645
Acc65I GGTACC 1 cut(s) 198
AccB1I GGYRCC 5 cut(s) 74, 198, 877, 1177, 1348
AccB7I CCANNNNNTGG 1 cut(s) 383
AccI GTMKAC 1 cut(s) 1633
AccII CGCG 2 cut(s) 14, 16
AciI CCGC 6 cut(s) 12, 16, 68, 681, 1175, 1486
AclWI GGATC 4 cut(s) 56, 940, 1293, 1306
AcsI RAATTY 2 cut(s) 410, 1477
AcuI CTGAAG 2 cut(s) 636, 912
AcyI GRCGYC 4 cut(s) 75, 123, 233, 1349
AfaI GTAC 3 cut(s) 155, 200, 419
AfiI CCNNNNNNNGG 6 cut(s) 71, 267, 302, 383, 669, 929
AgsI TTSAA 6 cut(s) 172, 325, 333, 415, 564, 1483
AjnI CCWGG 1 cut(s) 471
AjuI GAANNNNNNNTTGG 4 cut(s) 874, 906, 1277, 1309
AleI CACNNNNGTG 1 cut(s) 1016
Alw21I GWGCWC 1 cut(s) 45
Alw26I GTCTC 1 cut(s) 1442
AlwI GGATC 4 cut(s) 56, 940, 1293, 1306
AlwNI CAGNNNCTG 1 cut(s) 593
Ama87I CYCGRG 1 cut(s) 191
AoxI GGCC 4 cut(s) 265, 385, 655, 1458
ApeKI GCWGC 7 cut(s) 522, 544, 943, 1033, 1129, 1426, 1532
ApoI RAATTY 2 cut(s) 410, 1477
AseI ATTAAT 1 cut(s) 1542
Asp718I GGTACC 1 cut(s) 198
AspLEI GCGC 5 cut(s) 16, 77, 527, 1351, 1671
AspS9I GGNCC 2 cut(s) 137, 1459
AsuC2I CCSGG 2 cut(s) 72, 1432
AsuHPI GGTGA 3 cut(s) 103, 878, 1369
AsuNHI GCTAGC 1 cut(s) 1657
AvaI CYCGRG 1 cut(s) 191
AvaII GGWCC 1 cut(s) 137
BamHI GGATCC 1 cut(s) 1298
BanI GGYRCC 5 cut(s) 74, 198, 877, 1177, 1348
BanII GRGCYC 1 cut(s) 45
BbsI GAAGAC 1 cut(s) 642
Bbv12I GWGCWC 1 cut(s) 45
BbvCI CCTCAGC 2 cut(s) 836, 1584
BbvI GCAGC 7 cut(s) 531, 534, 930, 1020, 1116, 1438, 1519
BciT130I CCWGG 1 cut(s) 473
BcnI CCSGG 2 cut(s) 72, 1432
BcoDI GTCTC 1 cut(s) 1442
BfaI CTAG 2 cut(s) 827, 1658
BfmI CTRYAG 3 cut(s) 32, 1424, 1533
BfoI RGCGCY 2 cut(s) 78, 1352
BfuAI ACCTGC 1 cut(s) 1645
BlpI GCTNAGC 1 cut(s) 1273
Bme1390I CCNGG 3 cut(s) 72, 473, 1432
Bme18I GGWCC 1 cut(s) 137
BmeT110I CYCGRG 1 cut(s) 191
BmgT120I GGNCC 2 cut(s) 137, 1459
BmiI GGNNCC 8 cut(s) 76, 148, 200, 879, 1179, 1243, 1300, 1350
BmrFI CCNGG 3 cut(s) 72, 473, 1432
BmsI GCATC 2 cut(s) 1141, 1203
BmtI GCTAGC 1 cut(s) 1661
BpiI GAAGAC 1 cut(s) 642
BpmI CTGGAG 2 cut(s) 569, 665
Bpu10I CCTNAGC 3 cut(s) 836, 1467, 1584
Bpu1102I GCTNAGC 1 cut(s) 1273
BpuEI CTTGAG 1 cut(s) 29
BpuMI CCSGG 2 cut(s) 72, 1432
BsaHI GRCGYC 4 cut(s) 75, 123, 233, 1349
BsaJI CCNNGG 3 cut(s) 471, 1430, 1455
BsaWI WCCGGW 1 cut(s) 260
BsaXI ACNNNNNCTCC 4 cut(s) 49, 79, 481, 511
Bsc4I CCNNNNNNNGG 6 cut(s) 71, 267, 302, 383, 669, 929
Bse1I ACTGG 1 cut(s) 648
Bse3DI GCAATG 1 cut(s) 1578
BseBI CCWGG 1 cut(s) 473
BseDI CCNNGG 3 cut(s) 471, 1430, 1455
BseGI GGATG 3 cut(s) 1076, 1451, 1495
BseLI CCNNNNNNNGG 6 cut(s) 71, 267, 302, 383, 669, 929
BseMI GCAATG 1 cut(s) 1578
BseMII CTCAG 7 cut(s) 473, 837, 850, 1149, 1152, 1353, 1575
BseNI ACTGG 1 cut(s) 648
BseRI GAGGAG 5 cut(s) 41, 44, 80, 91, 94
BseXI GCAGC 7 cut(s) 531, 534, 930, 1020, 1116, 1438, 1519
BsgI GTGCAG 1 cut(s) 799
Bsh1236I CGCG 2 cut(s) 14, 16
Bsh1285I CGRYCG 1 cut(s) 939
BshFI GGCC 4 cut(s) 267, 387, 657, 1460
BshNI GGYRCC 5 cut(s) 74, 198, 877, 1177, 1348
BsiEI CGRYCG 1 cut(s) 939
BsiHKAI GWGCWC 1 cut(s) 45
BsiHKCI CYCGRG 1 cut(s) 191
BsiSI CCGG 4 cut(s) 71, 261, 268, 1432
BslFI GGGAC 1 cut(s) 1126
BslI CCNNNNNNNGG 6 cut(s) 71, 267, 302, 383, 669, 929
BsmAI GTCTC 1 cut(s) 1442
BsmFI GGGAC 1 cut(s) 1126
BsnI GGCC 4 cut(s) 267, 387, 657, 1460
BsoBI CYCGRG 1 cut(s) 191
Bsp1286I GDGCHC 1 cut(s) 45
Bsp143I GATC 6 cut(s) 61, 129, 449, 932, 1022, 1298
Bsp1720I GCTNAGC 1 cut(s) 1273
BspACI CCGC 6 cut(s) 12, 16, 68, 681, 1175, 1486
BspANI GGCC 4 cut(s) 267, 387, 657, 1460
BspCNI CTCAG 7 cut(s) 472, 838, 849, 1148, 1153, 1354, 1576
BspFNI CGCG 2 cut(s) 14, 16
BspLI GGNNCC 8 cut(s) 76, 148, 200, 879, 1179, 1243, 1300, 1350
BspMAI CTGCAG 3 cut(s) 36, 1428, 1537
BspMI ACCTGC 1 cut(s) 1645
BspOI GCTAGC 1 cut(s) 1661
BspPI GGATC 4 cut(s) 56, 940, 1293, 1306
BspT107I GGYRCC 5 cut(s) 74, 198, 877, 1177, 1348
BsrDI GCAATG 1 cut(s) 1578
BsrI ACTGG 1 cut(s) 648
BssECI CCNNGG 3 cut(s) 471, 1430, 1455
BssMI GATC 6 cut(s) 61, 129, 449, 932, 1022, 1298
BssNI GRCGYC 4 cut(s) 75, 123, 233, 1349
BssT1I CCWWGG 1 cut(s) 1455
Bst2UI CCWGG 1 cut(s) 473
Bst4CI ACNGT 6 cut(s) 151, 337, 541, 890, 959, 1015
Bst6I CTCTTC 1 cut(s) 1447
BstACI GRCGYC 4 cut(s) 75, 123, 233, 1349
BstAPI GCANNNNNTGC 1 cut(s) 593
BstC8I GCNNGC 4 cut(s) 429, 965, 1279, 1659
BstDEI CTNAG 9 cut(s) 459, 836, 846, 1135, 1161, 1273, 1362, 1467, 1584
BstF5I GGATG 3 cut(s) 1076, 1451, 1495
BstFNI CGCG 2 cut(s) 14, 16
BstH2I RGCGCY 2 cut(s) 78, 1352
BstHHI GCGC 5 cut(s) 16, 77, 527, 1351, 1671
BstKTI GATC 6 cut(s) 64, 132, 452, 935, 1025, 1301
BstMAI GTCTC 1 cut(s) 1442
BstMBI GATC 6 cut(s) 61, 129, 449, 932, 1022, 1298
BstMCI CGRYCG 1 cut(s) 939
BstMWI GCNNNNNNNGC 7 cut(s) 11, 40, 273, 593, 706, 1181, 1278
BstNI CCWGG 1 cut(s) 473
BstSCI CCNGG 3 cut(s) 70, 471, 1430
BstSFI CTRYAG 3 cut(s) 32, 1424, 1533
BstUI CGCG 2 cut(s) 14, 16
BstV1I GCAGC 7 cut(s) 531, 534, 930, 1020, 1116, 1438, 1519
BstV2I GAAGAC 1 cut(s) 642
BstX2I RGATCY 1 cut(s) 1298
BstYI RGATCY 1 cut(s) 1298
BsuRI GGCC 4 cut(s) 267, 387, 657, 1460
BtgZI GCGATG 1 cut(s) 1655
BtsCI GGATG 3 cut(s) 1076, 1451, 1495
BtsI GCAGTG 1 cut(s) 1596
BtsIMutI CAGTG 5 cut(s) 57, 641, 1143, 1596, 1659
BveI ACCTGC 1 cut(s) 1645
Cac8I GCNNGC 4 cut(s) 429, 965, 1279, 1659
CaiI CAGNNNCTG 1 cut(s) 593
CfoI GCGC 5 cut(s) 16, 77, 527, 1351, 1671
Cfr13I GGNCC 2 cut(s) 137, 1459
CseI GACGC 3 cut(s) 131, 241, 1025
Csp6I GTAC 3 cut(s) 154, 199, 418
CviAII CATG 6 cut(s) 309, 391, 569, 581, 1322, 1672
CviQI GTAC 3 cut(s) 154, 199, 418
DdeI CTNAG 9 cut(s) 459, 836, 846, 1135, 1161, 1273, 1362, 1467, 1584
DinI GGCGCC 2 cut(s) 76, 1350
DpnI GATC 6 cut(s) 63, 131, 451, 934, 1024, 1300
DpnII GATC 6 cut(s) 61, 129, 449, 932, 1022, 1298
DraI TTTAAA 1 cut(s) 1071
DrdI GACNNNNNNGTC 1 cut(s) 1013
DseDI GACNNNNNNGTC 1 cut(s) 1013
Eam1104I CTCTTC 1 cut(s) 1447
EarI CTCTTC 1 cut(s) 1447
EciI GGCGGA 1 cut(s) 57
Ecl136II GAGCTC 1 cut(s) 43
Eco130I CCWWGG 1 cut(s) 1455
Eco24I GRGCYC 1 cut(s) 45
Eco47I GGWCC 1 cut(s) 137
Eco53kI GAGCTC 1 cut(s) 43
Eco57I CTGAAG 2 cut(s) 636, 912
Eco88I CYCGRG 1 cut(s) 191
EcoICRI GAGCTC 1 cut(s) 43
EcoO109I RGGNCCY 1 cut(s) 1459
EcoRI GAATTC 1 cut(s) 410
EcoRII CCWGG 1 cut(s) 471
EcoT14I CCWWGG 1 cut(s) 1455
EcoT38I GRGCYC 1 cut(s) 45
EgeI GGCGCC 2 cut(s) 76, 1350
EheI GGCGCC 2 cut(s) 76, 1350
ErhI CCWWGG 1 cut(s) 1455
FaeI CATG 6 cut(s) 312, 394, 572, 584, 1325, 1675
FalI AAGNNNNNCTT 2 cut(s) 1256, 1288
FaqI GGGAC 1 cut(s) 1126
FatI CATG 6 cut(s) 308, 390, 568, 580, 1321, 1671
FauI CCCGC 2 cut(s) 674, 1479
FblI GTMKAC 1 cut(s) 1633
FokI GGATG 3 cut(s) 1063, 1438, 1502
FriOI GRGCYC 1 cut(s) 45
FspBI CTAG 2 cut(s) 827, 1658
GlaI GCGC 5 cut(s) 15, 76, 526, 1350, 1670
GsuI CTGGAG 2 cut(s) 569, 665
HaeII RGCGCY 2 cut(s) 78, 1352
HaeIII GGCC 4 cut(s) 267, 387, 657, 1460
HapII CCGG 4 cut(s) 71, 261, 268, 1432
HgaI GACGC 3 cut(s) 131, 241, 1025
HhaI GCGC 5 cut(s) 16, 77, 527, 1351, 1671
Hin1I GRCGYC 4 cut(s) 75, 123, 233, 1349
Hin1II CATG 6 cut(s) 312, 394, 572, 584, 1325, 1675
Hin6I GCGC 5 cut(s) 14, 75, 525, 1349, 1669
HinP1I GCGC 5 cut(s) 14, 75, 525, 1349, 1669
HindIII AAGCTT 4 cut(s) 429, 558, 1265, 1286
HpaII CCGG 4 cut(s) 71, 261, 268, 1432
HphI GGTGA 3 cut(s) 103, 878, 1369
Hpy166II GTNNAC 3 cut(s) 154, 368, 1634
Hpy188III TCNNGA 9 cut(s) 140, 377, 791, 930, 951, 986, 1018, 1187, 1302
Hpy8I GTNNAC 3 cut(s) 154, 368, 1634
Hpy99I CGWCG 2 cut(s) 125, 235
HpyAV CCTTC 8 cut(s) 31, 137, 155, 295, 607, 923, 1472, 1550
HpyCH4III ACNGT 6 cut(s) 151, 337, 541, 890, 959, 1015
HpyF10VI GCNNNNNNNGC 7 cut(s) 11, 40, 273, 593, 706, 1181, 1278
HpyF3I CTNAG 9 cut(s) 459, 836, 846, 1135, 1161, 1273, 1362, 1467, 1584
Hsp92I GRCGYC 4 cut(s) 75, 123, 233, 1349
Hsp92II CATG 6 cut(s) 312, 394, 572, 584, 1325, 1675
HspAI GCGC 5 cut(s) 14, 75, 525, 1349, 1669
KasI GGCGCC 2 cut(s) 74, 1348
KpnI GGTACC 1 cut(s) 202
Kzo9I GATC 6 cut(s) 61, 129, 449, 932, 1022, 1298
LmnI GCTCC 4 cut(s) 40, 54, 807, 1247
Lsp1109I GCAGC 7 cut(s) 531, 534, 930, 1020, 1116, 1438, 1519
LweI GCATC 2 cut(s) 1141, 1203
MaeI CTAG 2 cut(s) 827, 1658
MaeIII GTNAC 5 cut(s) 220, 497, 639, 822, 1357
MalI GATC 6 cut(s) 63, 131, 451, 934, 1024, 1300
MboI GATC 6 cut(s) 61, 129, 449, 932, 1022, 1298
MflI RGATCY 1 cut(s) 1298
MhlI GDGCHC 1 cut(s) 45
MluCI AATT 7 cut(s) 237, 410, 618, 818, 995, 1477, 1565
Mly113I GGCGCC 2 cut(s) 75, 1349
MlyI GAGTC 7 cut(s) 451, 494, 581, 599, 656, 796, 1444
MmeI TCCRAC 2 cut(s) 52, 1228
MseI TTAA 6 cut(s) 21, 177, 555, 900, 1070, 1542
MslI CAYNNNNRTG 2 cut(s) 1016, 1149
MspA1I CMGCKG 1 cut(s) 1175
MspI CCGG 4 cut(s) 71, 261, 268, 1432
MspR9I CCNGG 3 cut(s) 72, 473, 1432
MvaI CCWGG 1 cut(s) 473
MvnI CGCG 2 cut(s) 14, 16
MwoI GCNNNNNNNGC 7 cut(s) 11, 40, 273, 593, 706, 1181, 1278
NarI GGCGCC 2 cut(s) 75, 1349
NciI CCSGG 2 cut(s) 72, 1432
NdeII GATC 6 cut(s) 61, 129, 449, 932, 1022, 1298
NheI GCTAGC 1 cut(s) 1657
NlaIII CATG 6 cut(s) 312, 394, 572, 584, 1325, 1675
NlaIV GGNNCC 8 cut(s) 76, 148, 200, 879, 1179, 1243, 1300, 1350
NmuCI GTSAC 5 cut(s) 220, 497, 639, 822, 1357
OliI CACNNNNGTG 1 cut(s) 1016
PfeI GAWTC 3 cut(s) 165, 181, 1366
PflMI CCANNNNNTGG 1 cut(s) 383
PleI GAGTC 7 cut(s) 451, 494, 580, 598, 655, 795, 1443
PluTI GGCGCC 2 cut(s) 78, 1352
PpsI GAGTC 7 cut(s) 451, 494, 580, 598, 655, 795, 1443
PshBI ATTAAT 1 cut(s) 1542
Psp124BI GAGCTC 1 cut(s) 45
Psp6I CCWGG 1 cut(s) 471
PspGI CCWGG 1 cut(s) 471
PspN4I GGNNCC 8 cut(s) 76, 148, 200, 879, 1179, 1243, 1300, 1350
PspPI GGNCC 2 cut(s) 137, 1459
PstI CTGCAG 3 cut(s) 36, 1428, 1537
PstNI CAGNNNCTG 1 cut(s) 593
PsuI RGATCY 1 cut(s) 1298
RsaI GTAC 3 cut(s) 155, 200, 419
RsaNI GTAC 3 cut(s) 154, 199, 418
RseI CAYNNNNRTG 2 cut(s) 1016, 1149
SacI GAGCTC 1 cut(s) 45
SaqAI TTAA 6 cut(s) 21, 177, 555, 900, 1070, 1542
Sau3AI GATC 6 cut(s) 61, 129, 449, 932, 1022, 1298
Sau96I GGNCC 2 cut(s) 137, 1459
SchI GAGTC 7 cut(s) 451, 494, 581, 599, 656, 796, 1444
ScrFI CCNGG 3 cut(s) 72, 473, 1432
SduI GDGCHC 1 cut(s) 45
SfaNI GCATC 2 cut(s) 1141, 1203
SfcI CTRYAG 3 cut(s) 32, 1424, 1533
SfoI GGCGCC 2 cut(s) 76, 1350
SinI GGWCC 1 cut(s) 137
SmiMI CAYNNNNRTG 2 cut(s) 1016, 1149
SmlI CTYRAG 2 cut(s) 6, 44
SmoI CTYRAG 2 cut(s) 6, 44
Sse9I AATT 7 cut(s) 237, 410, 618, 818, 995, 1477, 1565
SsiI CCGC 6 cut(s) 12, 16, 68, 681, 1175, 1486
SspDI GGCGCC 2 cut(s) 74, 1348
SspI AATATT 1 cut(s) 1086
SspMI CTAG 2 cut(s) 827, 1658
SstI GAGCTC 1 cut(s) 45
StyD4I CCNGG 3 cut(s) 70, 471, 1430
StyI CCWWGG 1 cut(s) 1455
TaaI ACNGT 6 cut(s) 151, 337, 541, 890, 959, 1015
TaqI TCGA 5 cut(s) 120, 255, 279, 435, 448
TaqII GACCGA 1 cut(s) 925
TasI AATT 7 cut(s) 237, 410, 618, 818, 995, 1477, 1565
TatI WGTACW 1 cut(s) 417
TauI GCSGC 3 cut(s) 14, 19, 1178
TfiI GAWTC 3 cut(s) 165, 181, 1366
Tru1I TTAA 6 cut(s) 21, 177, 555, 900, 1070, 1542
Tru9I TTAA 6 cut(s) 21, 177, 555, 900, 1070, 1542
TscAI CASTG 5 cut(s) 64, 648, 1143, 1596, 1659
TseFI GTSAC 5 cut(s) 220, 497, 639, 822, 1357
TseI GCWGC 7 cut(s) 522, 544, 943, 1033, 1129, 1426, 1532
Tsp45I GTSAC 5 cut(s) 220, 497, 639, 822, 1357
TspDTI ATGAA 8 cut(s) 597, 708, 882, 965, 1102, 1107, 1434, 1524
TspGWI ACGGA 1 cut(s) 1234
TspRI CASTG 5 cut(s) 64, 648, 1143, 1596, 1659
Van91I CCANNNNNTGG 1 cut(s) 383
VpaK11BI GGWCC 1 cut(s) 137
VspI ATTAAT 1 cut(s) 1542
XapI RAATTY 2 cut(s) 410, 1477
XmiI GTMKAC 1 cut(s) 1633
XspI CTAG 2 cut(s) 827, 1658
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.