pycom15g22610

Transmembrane protein 53

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0014260
Physical Location & Seq
Forward (+)
16692529 .. 16694394
1866 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1194 bp
ATGGAAGCTCCGGCGAGAATTCTCAGTCTCAATCGCCAGCTCTTTGCCAGAACGACGCCGTCCGCCTTCGCTTTCTCCCCAAAACTCCGTCGGCCCGCAAATTCGTCGCTTATATTTCACACTCTCGCTCCAAACGGCGTCGGCAAAAGAACCCCCTTTTCTCTTTCGCTCGCGCAGTCGCACTCCTCGTTCTGCTTCAATCCGAACCCCTTCAAATCCCTCCCCCTTTCTCTCTCCTCTCAGTCGCCGTTCATCAGCTTCAACCACTCTCTCTCACCCTCAAACGACGACGTTTCCACCTCGGATCGGGCGTTCCACGGTGACGTTGACGGAAATGTGGGTTCTTTCGGGACCCACAGCCGTGAAGTGAGAGTGGTGTTGCTCGGCTGGCTCGGCGCCAAGACAAAGCATCTGAAGAGGTACGTGGAATGGTACAACTCCAGAGGCATTCACGCGGTGACCTTCGTCGTTGCTCCGAGAGAGGTGCTCTGGTTTGATTTGGGCCGCCGGGTCGAAAATCGGGTTTCAGACTTGGCCCGGGAGCTGGCTTCGTGGGTGGAGGATGGCGGAGAAAGGTCTTTGCTTTTTCACACTTTCAGCAATACGGGTTGGTTTGTGTATGGTGCCATTCTTGAAATTCTGCAGAGGAGGCAAAACCTGATGGAGAAGATTAAAGGGTGTGTGGTTGATTCAGGAGCAGCTGAACCTTTTAATCCTAAGGTGTGGGCATCGGGATTTTCTGCTGCGATTTTGAAAAAACGCAGTTCTTTAACAAGCGCTGCAATTGAGGCTGGAGAATTAAATGAGTCGGAAGATGCAGCAAGCTTGTCAAAGCTGCAAGAAGAGAAGCCTCCACTAGTCGAAGCCGTGGTTTTACTGATATTAGAGAAGCTGTTTTCAGTTGTTCTAAAGTTTCCTGATGTGGATAAGAGGTTAACGAAGGTAGTTTCTATTCTTTCAGAAAACAGTCCTGATTGCCCACAGCTTTACCTATACAGTACTGCTGATAAAGTCGTTCCACACCAGTCAATTGAGTCGTTCATGGCTGAGCAAAAGAGGAAGGGTAGAATTGTACGGTCCTTCAATTTCAGCACAACACCTCATGTGGATCACTTCAGGAATTTCCCTGACATATACTCTTCAGAGCTCGACGGTTTCTTGAAAGATTGTTTAACTACAGTCAAGACGATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF829

Protein Analysis

398

Amino Acids

44.28

Weight (kDa)

9.06

Isoelectric Point (pI)

47.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF829 PF05705 125 - 387 1.8e-61 Eukaryotic protein of unknown function (DUF829)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014260)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G18245
fragaria_vesca FvH4_1g13010
malus_domestica MD02G1144600.v1.1 MD15G1258300.v1.1
prunus_persica Prupe.7G157000_v2.0.a1 Prupe.7G157000_v2.0.a1
pyrus_communis pycom02g11450 pycom15g22610
rosa_chinensis RchiOBHm_Chr2g0101161
rosa_laevigata RLG00000017051
rosa_roxburghii Rroxscaffold_2G00141530
rosa_rugosa Rorug02G0100600
rosa_samantha Rh2AG144000 Rh2BG149000 Rh2CG150300 Rh2DG149500
rosa_wichuraiana Rw2G011290

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 395, 623
AccII CGCG 2 cut(s) 173, 455
AciI CCGC 5 cut(s) 63, 96, 455, 505, 567
AclWI GGATC 2 cut(s) 312, 1116
AcsI RAATTY 4 cut(s) 18, 100, 636, 1120
AcuI CTGAAG 3 cut(s) 434, 1099, 1125
AcyI GRCGYC 3 cut(s) 56, 138, 396
AdeI CACNNNGTG 1 cut(s) 457
AfaI GTAC 4 cut(s) 422, 434, 1000, 1074
AfeI AGCGCT 1 cut(s) 778
AfiI CCNNNNNNNGG 2 cut(s) 306, 544
AgsI TTSAA 7 cut(s) 199, 214, 262, 635, 754, 1084, 1162
AhlI ACTAGT 1 cut(s) 856
AleI CACNNNNGTG 1 cut(s) 360
AloI GAACNNNNNNTCC 2 cut(s) 296, 328
Alw21I GWGCWC 2 cut(s) 489, 1149
Alw26I GTCTC 1 cut(s) 32
AlwI GGATC 2 cut(s) 312, 1116
Ama87I CYCGRG 1 cut(s) 537
Aor51HI AGCGCT 1 cut(s) 778
AoxI GGCC 3 cut(s) 92, 502, 534
ApeKI GCWGC 5 cut(s) 698, 743, 779, 818, 835
ApoI RAATTY 4 cut(s) 18, 100, 636, 1120
Asp700I GAANNNNTTC 1 cut(s) 209
AspLEI GCGC 3 cut(s) 175, 398, 779
AspS9I GGNCC 5 cut(s) 93, 351, 502, 535, 1077
AsuC2I CCSGG 3 cut(s) 509, 538, 539
AsuHPI GGTGA 3 cut(s) 267, 332, 469
AvaI CYCGRG 1 cut(s) 537
AvaII GGWCC 2 cut(s) 351, 1077
AxyI CCTNAGG 1 cut(s) 717
BanI GGYRCC 2 cut(s) 395, 623
BanII GRGCYC 1 cut(s) 1149
Bbv12I GWGCWC 2 cut(s) 489, 1149
BbvI GCAGC 5 cut(s) 710, 730, 766, 822, 830
BccI CCATC 2 cut(s) 557, 655
BceAI ACGGC 5 cut(s) 43, 151, 232, 345, 851
BcgI CGANNNNNNTGC 4 cut(s) 87, 121, 466, 500
BcnI CCSGG 3 cut(s) 509, 538, 539
BcoDI GTCTC 1 cut(s) 32
BcuI ACTAGT 1 cut(s) 856
BfaI CTAG 1 cut(s) 857
BfmI CTRYAG 2 cut(s) 641, 1176
BfoI RGCGCY 2 cut(s) 399, 780
BisI GCNGC 6 cut(s) 505, 699, 744, 780, 819, 836
BlpI GCTNAGC 1 cut(s) 1047
BlsI GCNGC 6 cut(s) 506, 700, 745, 781, 820, 837
BmcAI AGTACT 1 cut(s) 1000
Bme1390I CCNGG 3 cut(s) 509, 538, 539
Bme18I GGWCC 2 cut(s) 351, 1077
BmeT110I CYCGRG 1 cut(s) 537
BmgT120I GGNCC 5 cut(s) 93, 351, 502, 535, 1077
BmiI GGNNCC 4 cut(s) 352, 353, 397, 625
BmrFI CCNGG 3 cut(s) 509, 538, 539
BmsI GCATC 3 cut(s) 418, 737, 805
BoxI GACNNNNGTC 1 cut(s) 464
BplI GAGNNNNNCTC 2 cut(s) 471, 503
BpmI CTGGAG 2 cut(s) 424, 813
Bpu1102I GCTNAGC 1 cut(s) 1047
BpuMI CCSGG 3 cut(s) 509, 538, 539
BsaAI YACGTR 1 cut(s) 424
BsaHI GRCGYC 3 cut(s) 56, 138, 396
BsaJI CCNNGG 4 cut(s) 300, 316, 537, 867
BsaXI ACNNNNNCTCC 2 cut(s) 112, 142
Bsc4I CCNNNNNNNGG 2 cut(s) 306, 544
Bse1I ACTGG 1 cut(s) 1024
Bse21I CCTNAGG 1 cut(s) 717
BseDI CCNNGG 4 cut(s) 300, 316, 537, 867
BseGI GGATG 1 cut(s) 568
BseLI CCNNNNNNNGG 2 cut(s) 306, 544
BseMII CTCAG 3 cut(s) 37, 254, 1038
BseNI ACTGG 1 cut(s) 1024
BseRI GAGGAG 3 cut(s) 175, 226, 661
BseXI GCAGC 5 cut(s) 710, 730, 766, 822, 830
Bsh1236I CGCG 2 cut(s) 173, 455
BshFI GGCC 3 cut(s) 94, 504, 536
BshNI GGYRCC 2 cut(s) 395, 623
BsiHKAI GWGCWC 2 cut(s) 489, 1149
BsiHKCI CYCGRG 1 cut(s) 537
BsiSI CCGG 3 cut(s) 11, 508, 538
BslFI GGGAC 1 cut(s) 364
BslI CCNNNNNNNGG 2 cut(s) 306, 544
BsmAI GTCTC 1 cut(s) 32
BsmFI GGGAC 1 cut(s) 364
BsmI GAATGC 1 cut(s) 447
BsnI GGCC 3 cut(s) 94, 504, 536
BsoBI CYCGRG 1 cut(s) 537
Bsp1286I GDGCHC 2 cut(s) 489, 1149
Bsp143I GATC 2 cut(s) 304, 1108
Bsp1720I GCTNAGC 1 cut(s) 1047
BspACI CCGC 5 cut(s) 63, 96, 455, 505, 567
BspANI GGCC 3 cut(s) 94, 504, 536
BspCNI CTCAG 3 cut(s) 36, 253, 1039
BspFNI CGCG 2 cut(s) 173, 455
BspLI GGNNCC 4 cut(s) 352, 353, 397, 625
BspMAI CTGCAG 1 cut(s) 645
BspPI GGATC 2 cut(s) 312, 1116
BspT107I GGYRCC 2 cut(s) 395, 623
BsrI ACTGG 1 cut(s) 1024
BssECI CCNNGG 4 cut(s) 300, 316, 537, 867
BssMI GATC 2 cut(s) 304, 1108
BssNI GRCGYC 3 cut(s) 56, 138, 396
Bst4CI ACNGT 6 cut(s) 320, 968, 998, 1077, 1154, 1180
Bst6I CTCTTC 3 cut(s) 410, 837, 1144
BstACI GRCGYC 3 cut(s) 56, 138, 396
BstBAI YACGTR 1 cut(s) 424
BstC8I GCNNGC 6 cut(s) 38, 96, 171, 389, 546, 823
BstDEI CTNAG 4 cut(s) 23, 240, 717, 1047
BstDSI CCRYGG 2 cut(s) 316, 867
BstEII GGTNACC 1 cut(s) 457
BstF5I GGATG 1 cut(s) 568
BstFNI CGCG 2 cut(s) 173, 455
BstH2I RGCGCY 2 cut(s) 399, 780
BstHHI GCGC 3 cut(s) 175, 398, 779
BstKTI GATC 2 cut(s) 307, 1111
BstMAI GTCTC 1 cut(s) 32
BstMBI GATC 2 cut(s) 304, 1108
BstMWI GCNNNNNNNGC 4 cut(s) 388, 393, 649, 788
BstPAI GACNNNNGTC 1 cut(s) 464
BstPI GGTNACC 1 cut(s) 457
BstSCI CCNGG 3 cut(s) 507, 536, 537
BstSFI CTRYAG 2 cut(s) 641, 1176
BstUI CGCG 2 cut(s) 173, 455
BstV1I GCAGC 5 cut(s) 710, 730, 766, 822, 830
Bsu36I CCTNAGG 1 cut(s) 717
BsuRI GGCC 3 cut(s) 94, 504, 536
BtgI CCRYGG 2 cut(s) 316, 867
BtsCI GGATG 1 cut(s) 568
Cac8I GCNNGC 6 cut(s) 38, 96, 171, 389, 546, 823
CfoI GCGC 3 cut(s) 175, 398, 779
Cfr13I GGNCC 5 cut(s) 93, 351, 502, 535, 1077
Cfr9I CCCGGG 1 cut(s) 537
CseI GACGC 2 cut(s) 64, 127
Csp6I GTAC 4 cut(s) 421, 433, 999, 1073
CviAII CATG 2 cut(s) 1042, 1103
CviQI GTAC 4 cut(s) 421, 433, 999, 1073
DdeI CTNAG 4 cut(s) 23, 240, 717, 1047
DinI GGCGCC 1 cut(s) 397
DpnI GATC 2 cut(s) 306, 1110
DpnII GATC 2 cut(s) 304, 1108
DraIII CACNNNGTG 1 cut(s) 457
Eam1104I CTCTTC 3 cut(s) 410, 837, 1144
EarI CTCTTC 3 cut(s) 410, 837, 1144
EciI GGCGGA 2 cut(s) 52, 582
Ecl136II GAGCTC 1 cut(s) 1147
Eco24I GRGCYC 1 cut(s) 1149
Eco47I GGWCC 2 cut(s) 351, 1077
Eco47III AGCGCT 1 cut(s) 778
Eco53kI GAGCTC 1 cut(s) 1147
Eco57I CTGAAG 3 cut(s) 434, 1099, 1125
Eco81I CCTNAGG 1 cut(s) 717
Eco88I CYCGRG 1 cut(s) 537
Eco91I GGTNACC 1 cut(s) 457
EcoICRI GAGCTC 1 cut(s) 1147
EcoO109I RGGNCCY 1 cut(s) 351
EcoO65I GGTNACC 1 cut(s) 457
EcoRI GAATTC 1 cut(s) 18
EcoT38I GRGCYC 1 cut(s) 1149
EgeI GGCGCC 1 cut(s) 397
EheI GGCGCC 1 cut(s) 397
FaeI CATG 2 cut(s) 1045, 1106
FaiI YATR 7 cut(s) 113, 621, 994, 1043, 1104, 1133, 1135
FaqI GGGAC 1 cut(s) 364
FatI CATG 2 cut(s) 1041, 1102
FauI CCCGC 1 cut(s) 103
Fnu4HI GCNGC 6 cut(s) 505, 699, 744, 780, 819, 836
FokI GGATG 1 cut(s) 575
FriOI GRGCYC 1 cut(s) 1149
Fsp4HI GCNGC 6 cut(s) 505, 699, 744, 780, 819, 836
FspBI CTAG 1 cut(s) 857
GlaI GCGC 3 cut(s) 174, 397, 778
GluI GCNGC 6 cut(s) 505, 699, 744, 780, 819, 836
GsuI CTGGAG 2 cut(s) 424, 813
HaeII RGCGCY 2 cut(s) 399, 780
HaeIII GGCC 3 cut(s) 94, 504, 536
HapII CCGG 3 cut(s) 11, 508, 538
HgaI GACGC 2 cut(s) 64, 127
HhaI GCGC 3 cut(s) 175, 398, 779
Hin1I GRCGYC 3 cut(s) 56, 138, 396
Hin1II CATG 2 cut(s) 1045, 1106
Hin6I GCGC 3 cut(s) 173, 396, 777
HinP1I GCGC 3 cut(s) 173, 396, 777
HincII GTYRAC 2 cut(s) 328, 936
HindII GTYRAC 2 cut(s) 328, 936
HindIII AAGCTT 1 cut(s) 823
HinfI GANTC 3 cut(s) 689, 806, 1034
HpaI GTTAAC 1 cut(s) 936
HpaII CCGG 3 cut(s) 11, 508, 538
HphI GGTGA 3 cut(s) 267, 332, 469
Hpy166II GTNNAC 2 cut(s) 328, 936
Hpy188I TCNGA 8 cut(s) 204, 304, 414, 477, 529, 811, 961, 1144
Hpy8I GTNNAC 2 cut(s) 328, 936
Hpy99I CGWCG 8 cut(s) 58, 93, 109, 143, 290, 293, 470, 1154
HpyAV CCTTC 6 cut(s) 76, 220, 472, 934, 1054, 1090
HpyCH4III ACNGT 6 cut(s) 320, 968, 998, 1077, 1154, 1180
HpyCH4IV ACGT 3 cut(s) 291, 324, 423
HpyCH4V TGCA 4 cut(s) 643, 782, 818, 838
HpyF10VI GCNNNNNNNGC 4 cut(s) 388, 393, 649, 788
HpyF3I CTNAG 4 cut(s) 23, 240, 717, 1047
HpySE526I ACGT 3 cut(s) 291, 324, 423
Hsp92I GRCGYC 3 cut(s) 56, 138, 396
Hsp92II CATG 2 cut(s) 1045, 1106
HspAI GCGC 3 cut(s) 173, 396, 777
KasI GGCGCC 1 cut(s) 395
KflI GGGWCCC 1 cut(s) 351
KspAI GTTAAC 1 cut(s) 936
Kzo9I GATC 2 cut(s) 304, 1108
LmnI GCTCC 5 cut(s) 13, 133, 478, 541, 695
Lsp1109I GCAGC 5 cut(s) 710, 730, 766, 822, 830
LweI GCATC 3 cut(s) 418, 737, 805
MaeI CTAG 1 cut(s) 857
MaeII ACGT 3 cut(s) 291, 324, 423
MaeIII GTNAC 2 cut(s) 320, 457
MalI GATC 2 cut(s) 306, 1110
MboI GATC 2 cut(s) 304, 1108
MboII GAAGA 5 cut(s) 427, 679, 824, 854, 1131
MfeI CAATTG 2 cut(s) 783, 1029
MhlI GDGCHC 2 cut(s) 489, 1149
MluCI AATT 9 cut(s) 18, 100, 636, 783, 797, 1029, 1068, 1084, 1120
Mly113I GGCGCC 1 cut(s) 396
MlyI GAGTC 2 cut(s) 815, 1043
MmeI TCCRAC 1 cut(s) 789
MroXI GAANNNNTTC 1 cut(s) 209
MseI TTAA 6 cut(s) 672, 711, 770, 800, 935, 1172
MslI CAYNNNNRTG 1 cut(s) 360
MspA1I CMGCKG 1 cut(s) 701
MspI CCGG 3 cut(s) 11, 508, 538
MspR9I CCNGG 3 cut(s) 509, 538, 539
MunI CAATTG 2 cut(s) 783, 1029
Mva1269I GAATGC 1 cut(s) 447
MvnI CGCG 2 cut(s) 173, 455
MwoI GCNNNNNNNGC 4 cut(s) 388, 393, 649, 788
NarI GGCGCC 1 cut(s) 396
NciI CCSGG 3 cut(s) 509, 538, 539
NdeII GATC 2 cut(s) 304, 1108
NlaIII CATG 2 cut(s) 1045, 1106
NlaIV GGNNCC 4 cut(s) 352, 353, 397, 625
NmeAIII GCCGAG 2 cut(s) 363, 372
NmuCI GTSAC 2 cut(s) 320, 457
OliI CACNNNNGTG 1 cut(s) 360
PcsI WCGNNNNNNNCGW 2 cut(s) 185, 308
PctI GAATGC 1 cut(s) 447
PdmI GAANNNNTTC 1 cut(s) 209
PfeI GAWTC 1 cut(s) 689
PflFI GACNNNGTC 1 cut(s) 58
PkrI GCNGC 6 cut(s) 506, 700, 745, 781, 820, 837
PleI GAGTC 2 cut(s) 814, 1042
PluTI GGCGCC 1 cut(s) 399
PpsI GAGTC 2 cut(s) 814, 1042
Ppu21I YACGTR 1 cut(s) 424
PpuMI RGGWCCY 1 cut(s) 351
PshAI GACNNNNGTC 1 cut(s) 464
Psp124BI GAGCTC 1 cut(s) 1149
Psp5II RGGWCCY 1 cut(s) 351
PspEI GGTNACC 1 cut(s) 457
PspN4I GGNNCC 4 cut(s) 352, 353, 397, 625
PspPI GGNCC 5 cut(s) 93, 351, 502, 535, 1077
PspPPI RGGWCCY 1 cut(s) 351
PstI CTGCAG 1 cut(s) 645
PsyI GACNNNGTC 1 cut(s) 58
PvuII CAGCTG 1 cut(s) 701
RsaI GTAC 4 cut(s) 422, 434, 1000, 1074
RsaNI GTAC 4 cut(s) 421, 433, 999, 1073
RseI CAYNNNNRTG 1 cut(s) 360
SacI GAGCTC 1 cut(s) 1149
SaqAI TTAA 6 cut(s) 672, 711, 770, 800, 935, 1172
SatI GCNGC 6 cut(s) 505, 699, 744, 780, 819, 836
Sau3AI GATC 2 cut(s) 304, 1108
Sau96I GGNCC 5 cut(s) 93, 351, 502, 535, 1077
ScaI AGTACT 1 cut(s) 1000
SchI GAGTC 2 cut(s) 815, 1043
ScrFI CCNGG 3 cut(s) 509, 538, 539
SduI GDGCHC 2 cut(s) 489, 1149
SfaNI GCATC 3 cut(s) 418, 737, 805
SfcI CTRYAG 2 cut(s) 641, 1176
SfoI GGCGCC 1 cut(s) 397
SinI GGWCC 2 cut(s) 351, 1077
SmaI CCCGGG 1 cut(s) 539
SmiMI CAYNNNNRTG 1 cut(s) 360
SpeI ACTAGT 1 cut(s) 856
Sse9I AATT 9 cut(s) 18, 100, 636, 783, 797, 1029, 1068, 1084, 1120
SsiI CCGC 5 cut(s) 63, 96, 455, 505, 567
SspDI GGCGCC 1 cut(s) 395
SspMI CTAG 1 cut(s) 857
SstI GAGCTC 1 cut(s) 1149
StyD4I CCNGG 3 cut(s) 507, 536, 537
TaaI ACNGT 6 cut(s) 320, 968, 998, 1077, 1154, 1180
TaiI ACGT 3 cut(s) 294, 327, 426
TaqI TCGA 3 cut(s) 513, 861, 1149
TasI AATT 9 cut(s) 18, 100, 636, 783, 797, 1029, 1068, 1084, 1120
TatI WGTACW 1 cut(s) 998
TauI GCSGC 1 cut(s) 507
TfiI GAWTC 1 cut(s) 689
Tru1I TTAA 6 cut(s) 672, 711, 770, 800, 935, 1172
Tru9I TTAA 6 cut(s) 672, 711, 770, 800, 935, 1172
TseFI GTSAC 2 cut(s) 320, 457
TseI GCWGC 5 cut(s) 698, 743, 779, 818, 835
Tsp45I GTSAC 2 cut(s) 320, 457
TspDTI ATGAA 2 cut(s) 241, 1030
TspGWI ACGGA 2 cut(s) 77, 345
TspMI CCCGGG 1 cut(s) 537
Tth111I GACNNNGTC 1 cut(s) 58
VpaK11BI GGWCC 2 cut(s) 351, 1077
XapI RAATTY 4 cut(s) 18, 100, 636, 1120
XmaI CCCGGG 1 cut(s) 537
XmnI GAANNNNTTC 1 cut(s) 209
XspI CTAG 1 cut(s) 857
ZrmI AGTACT 1 cut(s) 1000
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.