pycom15g22900

Protein N-lysine methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0012035
Physical Location & Seq
Forward (+)
16939293 .. 16940093
801 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 801 bp
ATGGCGGATCAAGAAGGTGGTGATTCTGAAGACGATGCAATACTGATCCCCGAGACCGCAACGCTCCTTCCATCGACTCAACATTGCCTCCACTCCATCAACTCAGTCGTCTTTATCCGGGAGCTCCGGTCTCAAGGCCTCTCTTTCCAGCTCTGGCCGGCCGCCACCACGATCCTCACTCTCCTCGACGGCCACCACGGCGACCCTAACACCAGTCCCCTGGGACCCACACTCTCGGCCTTGGACATGCGGAAGCGCCCTCTCAAAATCCTCGAGCTCGGCTCCGGAACCGGCCTCGTCGGAATCGCCGCCGCAGCCACGCTCGGGGCTACCGTCACGGTCACTGACCTCCCACACGTCATCCCTAACCTTTTATTCAACGTGGAAGCGAACGCCTCGGTATTGGAAGCCAACGGCGGGATTGTCCAAGTGGCGGCGCTGGGGTGGGGGGAAGCGGGGGACGTGGAGGTGATTGGGCGCGAATTTGATCTTATTCTGGCGTCGGATGTAGTGTATCACGATCATCTCTACGAGCCGTTGCTCAAAACGTTGCGTTTGCTGATATTAGGTGAGGCGAAGGAGAATGAGGAGGGAAGGGTTTTTGTGATGGCCCACCTGAGGCGGTGGAAGAAGGAGTCGGCTTTCTTCAAGAAGGCCAGGAAGGTTTTTGAGGTGGAGGTCTTGCACGTGGACCCCCCGTGCCATGGCTCCAGGGTTGGAGTTACTGTTTTCCGTTTTACCGCGAAGAAGTCGGGTCGGAAAGTGTTGAATTCTACTCACAATACCAATGTCATTGCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_16

Protein Analysis

267

Amino Acids

28.82

Weight (kDa)

5.99

Isoelectric Point (pI)

31.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_16 PF10294 37 - 220 3.3e-30 Lysine methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012035)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G50850 AT5G49560
fragaria_vesca FvH4_1g13310
malus_domestica MD02G1147500.v1.1 MD15G1261800.v1.1
prunus_persica Prupe.7G154600_v2.0.a1
pyrus_communis pycom15g22900
rosa_chinensis RchiOBHm_Chr2g0101571
rosa_laevigata RLG00000017091
rosa_multiflora Rmu_co8339275.1_g000001 Rmu_sc0006153.1_g000007 Rmu_sc0019613.1_g000002
rosa_roxburghii Rroxscaffold_2G00141180
rosa_rugosa Rorug02G0097300
rosa_samantha Rh2AG148100 Rh2BG153500 Rh2CG154000 Rh2DG153800
rosa_wichuraiana Rw2G011550

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 480, 743
AccIII TCCGGA 1 cut(s) 284
AclI AACGTT 1 cut(s) 548
AclWI GGATC 3 cut(s) 15, 40, 166
AcoI YGGCCR 3 cut(s) 155, 159, 190
AcsI RAATTY 2 cut(s) 482, 769
AcuI CTGAAG 1 cut(s) 48
AcvI CACGTG 1 cut(s) 688
AcyI GRCGYC 1 cut(s) 500
AfiI CCNNNNNNNGG 5 cut(s) 324, 417, 433, 618, 704
AflIII ACRYGT 1 cut(s) 355
AgsI TTSAA 3 cut(s) 379, 649, 769
AjiI CACGTC 2 cut(s) 358, 463
AjnI CCWGG 3 cut(s) 219, 656, 710
AluBI AGCT 3 cut(s) 124, 151, 277
AluI AGCT 3 cut(s) 124, 151, 277
Alw21I GWGCWC 2 cut(s) 126, 279
Alw26I GTCTC 2 cut(s) 47, 135
AlwI GGATC 3 cut(s) 15, 40, 166
Ama87I CYCGRG 3 cut(s) 50, 272, 323
Aor13HI TCCGGA 1 cut(s) 284
AoxI GGCC 8 cut(s) 136, 155, 159, 190, 237, 292, 609, 654
ApeKI GCWGC 1 cut(s) 314
ApoI RAATTY 2 cut(s) 482, 769
AspLEI GCGC 3 cut(s) 258, 439, 480
AspS9I GGNCC 3 cut(s) 224, 610, 691
AsuC2I CCSGG 1 cut(s) 119
AsuHPI GGTGA 3 cut(s) 32, 481, 581
AvaI CYCGRG 3 cut(s) 50, 272, 323
AvaII GGWCC 2 cut(s) 224, 691
AxyI CCTNAGG 1 cut(s) 617
BanII GRGCYC 2 cut(s) 126, 279
BbrPI CACGTG 1 cut(s) 688
BbsI GAAGAC 1 cut(s) 36
Bbv12I GWGCWC 2 cut(s) 126, 279
BbvI GCAGC 1 cut(s) 326
BccI CCATC 3 cut(s) 79, 104, 601
BceAI ACGGC 4 cut(s) 205, 214, 430, 520
BciT130I CCWGG 3 cut(s) 221, 658, 712
BcnI CCSGG 1 cut(s) 119
BcoDI GTCTC 2 cut(s) 47, 135
BfoI RGCGCY 2 cut(s) 259, 440
BglI GCCNNNNNGGC 1 cut(s) 198
BisI GCNGC 5 cut(s) 162, 309, 312, 315, 435
BlsI GCNGC 5 cut(s) 163, 310, 313, 316, 436
Bme1390I CCNGG 4 cut(s) 119, 221, 658, 712
Bme18I GGWCC 2 cut(s) 224, 691
BmeT110I CYCGRG 3 cut(s) 50, 272, 323
BmgBI CACGTC 2 cut(s) 358, 463
BmgT120I GGNCC 3 cut(s) 224, 610, 691
BmiI GGNNCC 6 cut(s) 225, 226, 283, 289, 693, 709
BmrFI CCNGG 4 cut(s) 119, 221, 658, 712
BmsI GCATC 1 cut(s) 25
BpiI GAAGAC 1 cut(s) 36
BplI GAGNNNNNCTC 2 cut(s) 266, 298
BpmI CTGGAG 1 cut(s) 694
BpuEI CTTGAG 1 cut(s) 117
BpuMI CCSGG 1 cut(s) 119
BsaAI YACGTR 1 cut(s) 688
BsaHI GRCGYC 1 cut(s) 500
BsaI GGTCTC 2 cut(s) 47, 135
BsaJI CCNNGG 7 cut(s) 196, 219, 220, 240, 396, 703, 711
BsaWI WCCGGW 2 cut(s) 126, 284
BsaXI ACNNNNNCTCC 6 cut(s) 72, 102, 113, 143, 711, 741
Bsc4I CCNNNNNNNGG 5 cut(s) 324, 417, 433, 618, 704
Bse118I RCCGGY 2 cut(s) 157, 290
Bse1I ACTGG 1 cut(s) 213
Bse21I CCTNAGG 1 cut(s) 617
Bse3DI GCAATG 2 cut(s) 82, 792
BseAI TCCGGA 1 cut(s) 284
BseBI CCWGG 3 cut(s) 221, 658, 712
BseDI CCNNGG 7 cut(s) 196, 219, 220, 240, 396, 703, 711
BseGI GGATG 2 cut(s) 360, 511
BseLI CCNNNNNNNGG 5 cut(s) 324, 417, 433, 618, 704
BseMI GCAATG 2 cut(s) 82, 792
BseMII CTCAG 2 cut(s) 117, 608
BseNI ACTGG 1 cut(s) 213
BseRI GAGGAG 2 cut(s) 173, 602
BseX3I CGGCCG 1 cut(s) 159
BseXI GCAGC 1 cut(s) 326
BseYI CCCAGC 1 cut(s) 439
Bsh1236I CGCG 2 cut(s) 480, 743
Bsh1285I CGRYCG 1 cut(s) 162
BshFI GGCC 8 cut(s) 138, 157, 161, 192, 239, 294, 611, 656
BsiEI CGRYCG 1 cut(s) 162
BsiHKAI GWGCWC 2 cut(s) 126, 279
BsiHKCI CYCGRG 3 cut(s) 50, 272, 323
BsiSI CCGG 5 cut(s) 118, 127, 158, 285, 291
BslFI GGGAC 3 cut(s) 201, 237, 473
BslI CCNNNNNNNGG 5 cut(s) 324, 417, 433, 618, 704
BsmAI GTCTC 2 cut(s) 47, 135
BsmFI GGGAC 3 cut(s) 201, 237, 473
BsnI GGCC 8 cut(s) 138, 157, 161, 192, 239, 294, 611, 656
Bso31I GGTCTC 2 cut(s) 47, 135
BsoBI CYCGRG 3 cut(s) 50, 272, 323
Bsp1286I GDGCHC 2 cut(s) 126, 279
Bsp13I TCCGGA 1 cut(s) 284
Bsp143I GATC 5 cut(s) 7, 45, 171, 487, 520
Bsp19I CCATGG 1 cut(s) 703
BspANI GGCC 8 cut(s) 138, 157, 161, 192, 239, 294, 611, 656
BspCNI CTCAG 2 cut(s) 116, 609
BspEI TCCGGA 1 cut(s) 284
BspFNI CGCG 2 cut(s) 480, 743
BspLI GGNNCC 6 cut(s) 225, 226, 283, 289, 693, 709
BspPI GGATC 3 cut(s) 15, 40, 166
BspTNI GGTCTC 2 cut(s) 47, 135
BsrDI GCAATG 2 cut(s) 82, 792
BsrFI RCCGGY 2 cut(s) 157, 290
BsrI ACTGG 1 cut(s) 213
BssAI RCCGGY 2 cut(s) 157, 290
BssECI CCNNGG 7 cut(s) 196, 219, 220, 240, 396, 703, 711
BssMI GATC 5 cut(s) 7, 45, 171, 487, 520
BssNI GRCGYC 1 cut(s) 500
BssT1I CCWWGG 2 cut(s) 240, 703
Bst2UI CCWGG 3 cut(s) 221, 658, 712
Bst4CI ACNGT 3 cut(s) 334, 340, 727
BstACI GRCGYC 1 cut(s) 500
BstBAI YACGTR 1 cut(s) 688
BstC8I GCNNGC 1 cut(s) 159
BstDEI CTNAG 2 cut(s) 103, 617
BstDSI CCRYGG 2 cut(s) 196, 703
BstF5I GGATG 2 cut(s) 360, 511
BstFNI CGCG 2 cut(s) 480, 743
BstH2I RGCGCY 2 cut(s) 259, 440
BstHHI GCGC 3 cut(s) 258, 439, 480
BstKTI GATC 5 cut(s) 10, 48, 174, 490, 523
BstMAI GTCTC 2 cut(s) 47, 135
BstMBI GATC 5 cut(s) 7, 45, 171, 487, 520
BstMCI CGRYCG 1 cut(s) 162
BstMWI GCNNNNNNNGC 2 cut(s) 198, 314
BstNI CCWGG 3 cut(s) 221, 658, 712
BstNSI RCATGY 1 cut(s) 250
BstSCI CCNGG 4 cut(s) 117, 219, 656, 710
BstUI CGCG 2 cut(s) 480, 743
BstV1I GCAGC 1 cut(s) 326
BstV2I GAAGAC 1 cut(s) 36
BstXI CCANNNNNNTGG 1 cut(s) 220
BstZI CGGCCG 1 cut(s) 159
Bsu36I CCTNAGG 1 cut(s) 617
BsuRI GGCC 8 cut(s) 138, 157, 161, 192, 239, 294, 611, 656
BtgI CCRYGG 2 cut(s) 196, 703
BtrI CACGTC 2 cut(s) 358, 463
BtsCI GGATG 2 cut(s) 360, 511
BtsIMutI CAGTG 1 cut(s) 342
Cac8I GCNNGC 1 cut(s) 159
CfoI GCGC 3 cut(s) 258, 439, 480
Cfr10I RCCGGY 2 cut(s) 157, 290
Cfr13I GGNCC 3 cut(s) 224, 610, 691
CseI GACGC 1 cut(s) 489
CviAII CATG 2 cut(s) 247, 704
DdeI CTNAG 2 cut(s) 103, 617
DpnI GATC 5 cut(s) 9, 47, 173, 489, 522
DpnII GATC 5 cut(s) 7, 45, 171, 487, 520
EaeI YGGCCR 3 cut(s) 155, 159, 190
EagI CGGCCG 1 cut(s) 159
EciI GGCGGA 1 cut(s) 20
Ecl136II GAGCTC 2 cut(s) 124, 277
EclXI CGGCCG 1 cut(s) 159
Eco130I CCWWGG 2 cut(s) 240, 703
Eco147I AGGCCT 1 cut(s) 138
Eco24I GRGCYC 2 cut(s) 126, 279
Eco31I GGTCTC 2 cut(s) 47, 135
Eco47I GGWCC 2 cut(s) 224, 691
Eco52I CGGCCG 1 cut(s) 159
Eco53kI GAGCTC 2 cut(s) 124, 277
Eco57I CTGAAG 1 cut(s) 48
Eco72I CACGTG 1 cut(s) 688
Eco81I CCTNAGG 1 cut(s) 617
Eco88I CYCGRG 3 cut(s) 50, 272, 323
EcoICRI GAGCTC 2 cut(s) 124, 277
EcoO109I RGGNCCY 1 cut(s) 224
EcoRI GAATTC 1 cut(s) 769
EcoRII CCWGG 3 cut(s) 219, 656, 710
EcoT14I CCWWGG 2 cut(s) 240, 703
EcoT38I GRGCYC 2 cut(s) 126, 279
ErhI CCWWGG 2 cut(s) 240, 703
FaeI CATG 2 cut(s) 250, 707
FaiI YATR 2 cut(s) 248, 705
FaqI GGGAC 3 cut(s) 201, 237, 473
FatI CATG 2 cut(s) 246, 703
FauI CCCGC 2 cut(s) 410, 448
Fnu4HI GCNGC 5 cut(s) 162, 309, 312, 315, 435
FokI GGATG 2 cut(s) 347, 518
FriOI GRGCYC 2 cut(s) 126, 279
FseI GGCCGGCC 1 cut(s) 161
Fsp4HI GCNGC 5 cut(s) 162, 309, 312, 315, 435
GlaI GCGC 3 cut(s) 257, 438, 479
GluI GCNGC 5 cut(s) 162, 309, 312, 315, 435
GsaI CCCAGC 1 cut(s) 443
GsuI CTGGAG 1 cut(s) 694
HaeII RGCGCY 2 cut(s) 259, 440
HaeIII GGCC 8 cut(s) 138, 157, 161, 192, 239, 294, 611, 656
HapII CCGG 5 cut(s) 118, 127, 158, 285, 291
HgaI GACGC 1 cut(s) 489
HhaI GCGC 3 cut(s) 258, 439, 480
Hin1I GRCGYC 1 cut(s) 500
Hin1II CATG 2 cut(s) 250, 707
Hin6I GCGC 3 cut(s) 256, 437, 478
HinP1I GCGC 3 cut(s) 256, 437, 478
HinfI GANTC 4 cut(s) 23, 76, 303, 635
HpaII CCGG 5 cut(s) 118, 127, 158, 285, 291
HphI GGTGA 3 cut(s) 32, 481, 581
Hpy166II GTNNAC 1 cut(s) 691
Hpy188I TCNGA 4 cut(s) 28, 302, 505, 759
Hpy188III TCNNGA 4 cut(s) 11, 285, 518, 649
Hpy8I GTNNAC 1 cut(s) 691
Hpy99I CGWCG 3 cut(s) 191, 302, 505
HpyAV CCTTC 7 cut(s) 8, 77, 571, 588, 625, 646, 655
HpyCH4III ACNGT 3 cut(s) 334, 340, 727
HpyCH4IV ACGT 5 cut(s) 357, 381, 462, 548, 687
HpyCH4V TGCA 2 cut(s) 38, 685
HpyF10VI GCNNNNNNNGC 2 cut(s) 198, 314
HpyF3I CTNAG 2 cut(s) 103, 617
HpySE526I ACGT 5 cut(s) 357, 381, 462, 548, 687
Hsp92I GRCGYC 1 cut(s) 500
Hsp92II CATG 2 cut(s) 250, 707
HspAI GCGC 3 cut(s) 256, 437, 478
KflI GGGWCCC 1 cut(s) 224
Kpn2I TCCGGA 1 cut(s) 284
KroI GCCGGC 1 cut(s) 157
KroNI GCCGGC 1 cut(s) 159
Kzo9I GATC 5 cut(s) 7, 45, 171, 487, 520
LmnI GCTCC 5 cut(s) 69, 121, 129, 287, 713
Lsp1109I GCAGC 1 cut(s) 326
LweI GCATC 1 cut(s) 25
MaeII ACGT 5 cut(s) 357, 381, 462, 548, 687
MaeIII GTNAC 3 cut(s) 334, 340, 721
MalI GATC 5 cut(s) 9, 47, 173, 489, 522
MboI GATC 5 cut(s) 7, 45, 171, 487, 520
MboII GAAGA 4 cut(s) 41, 637, 640, 757
MhlI GDGCHC 2 cut(s) 126, 279
MluCI AATT 2 cut(s) 482, 769
MlyI GAGTC 2 cut(s) 70, 644
MmeI TCCRAC 4 cut(s) 280, 483, 697, 737
MroI TCCGGA 1 cut(s) 284
MroNI GCCGGC 1 cut(s) 157
MspI CCGG 5 cut(s) 118, 127, 158, 285, 291
MspR9I CCNGG 4 cut(s) 119, 221, 658, 712
MvaI CCWGG 3 cut(s) 221, 658, 712
MvnI CGCG 2 cut(s) 480, 743
MwoI GCNNNNNNNGC 2 cut(s) 198, 314
NaeI GCCGGC 1 cut(s) 159
NciI CCSGG 1 cut(s) 119
NcoI CCATGG 1 cut(s) 703
NdeII GATC 5 cut(s) 7, 45, 171, 487, 520
NgoMIV GCCGGC 1 cut(s) 157
NlaIII CATG 2 cut(s) 250, 707
NlaIV GGNNCC 6 cut(s) 225, 226, 283, 289, 693, 709
NmeAIII GCCGAG 2 cut(s) 215, 258
NmuCI GTSAC 2 cut(s) 334, 340
NspI RCATGY 1 cut(s) 250
PaeR7I CTCGAG 1 cut(s) 272
PasI CCCWGGG 1 cut(s) 220
PceI AGGCCT 1 cut(s) 138
PcsI WCGNNNNNNNCGW 1 cut(s) 330
PdiI GCCGGC 1 cut(s) 159
PfeI GAWTC 2 cut(s) 23, 303
PfoI TCCNGGA 1 cut(s) 117
PkrI GCNGC 5 cut(s) 163, 310, 313, 316, 436
PleI GAGTC 2 cut(s) 70, 643
PmaCI CACGTG 1 cut(s) 688
PmlI CACGTG 1 cut(s) 688
PpsI GAGTC 2 cut(s) 70, 643
Ppu21I YACGTR 1 cut(s) 688
PpuMI RGGWCCY 1 cut(s) 224
Psp124BI GAGCTC 2 cut(s) 126, 279
Psp1406I AACGTT 1 cut(s) 548
Psp5II RGGWCCY 1 cut(s) 224
Psp6I CCWGG 3 cut(s) 219, 656, 710
PspCI CACGTG 1 cut(s) 688
PspFI CCCAGC 1 cut(s) 439
PspGI CCWGG 3 cut(s) 219, 656, 710
PspN4I GGNNCC 6 cut(s) 225, 226, 283, 289, 693, 709
PspPI GGNCC 3 cut(s) 224, 610, 691
PspPPI RGGWCCY 1 cut(s) 224
PspXI VCTCGAGB 1 cut(s) 272
RigI GGCCGGCC 1 cut(s) 161
SacI GAGCTC 2 cut(s) 126, 279
SatI GCNGC 5 cut(s) 162, 309, 312, 315, 435
Sau3AI GATC 5 cut(s) 7, 45, 171, 487, 520
Sau96I GGNCC 3 cut(s) 224, 610, 691
SchI GAGTC 2 cut(s) 70, 644
ScrFI CCNGG 4 cut(s) 119, 221, 658, 712
SduI GDGCHC 2 cut(s) 126, 279
SfaNI GCATC 1 cut(s) 25
Sfr274I CTCGAG 1 cut(s) 272
SinI GGWCC 2 cut(s) 224, 691
SlaI CTCGAG 1 cut(s) 272
SmlI CTYRAG 2 cut(s) 132, 272
SmoI CTYRAG 2 cut(s) 132, 272
Sse9I AATT 2 cut(s) 482, 769
SseBI AGGCCT 1 cut(s) 138
SstI GAGCTC 2 cut(s) 126, 279
StuI AGGCCT 1 cut(s) 138
StyD4I CCNGG 4 cut(s) 117, 219, 656, 710
StyI CCWWGG 2 cut(s) 240, 703
TaaI ACNGT 3 cut(s) 334, 340, 727
TaiI ACGT 5 cut(s) 360, 384, 465, 551, 690
TaqI TCGA 3 cut(s) 74, 186, 273
TasI AATT 2 cut(s) 482, 769
TauI GCSGC 4 cut(s) 164, 311, 314, 437
TfiI GAWTC 2 cut(s) 23, 303
TscAI CASTG 1 cut(s) 349
TseFI GTSAC 2 cut(s) 334, 340
TseI GCWGC 1 cut(s) 314
Tsp45I GTSAC 2 cut(s) 334, 340
TspGWI ACGGA 1 cut(s) 722
TspRI CASTG 1 cut(s) 349
VpaK11BI GGWCC 2 cut(s) 224, 691
XapI RAATTY 2 cut(s) 482, 769
XceI RCATGY 1 cut(s) 250
XhoI CTCGAG 1 cut(s) 272
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.