pycom15g23490

DA1-related 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0004156
Physical Location & Seq
Reverse (-)
17578356 .. 17581974
3619 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1626 bp
ATGGGTTGGCTAACTAAGATTCTTAAAGGTTCTGGCCATAAGGGTCATTCTCATAAGAAGAAAATTAGAGAGGATAGAACATGGGACGAGCCTCGTGATTCTGTGGAAGAAGACATAGATGTTGCTATTGCACTGTCCCTTTCAGAACTAGATCAAAAAGGAAAAAATGTAATCGAAGATGAATCTCAATCAGAGGATGACGAACAACCGGCTAAAGTAGAATCAGACGATGATGAACAATCATCTGAAGTTGAATCAAACGATGATGAACGATCTGCCAAAGTTGAATCAGATGATGATGCTCGATCGGCCAAAGTTCAGTCGGACGATGATGAAAAACCCGCAAAATTTCACTACGAGGAAGATGAACAAAGTGCTAAAGTTCAATTGGAGGAAGATGAACAACTTGCAAAGGCTATTCAAGAAAGTTTGATCATGGGTTCTCCTCCTGGACATGATAATGGCAATATATTTCAACCTTTTTCATTCTTCACGCCAGCTGGGTACAGGACATGTGCTGGCTGCAAGTCGGGAATTGGCCATGGACGATATTTGAGTTGCATGGGTGCTGTTTGGCATCCAGAATGCTTCCGTTGCCGTGCTTGCAATCTTCCAATTACTGATTATGAGTATTCTATGTCTGGGAATCACCCTTACCACAAATCCTGCTATAAGGAGCAGCATCACCCAAAATGCGATGTTTGCAAGAGTTTCATCCCAACAAATTCAGCTGGGCTTATTGAGTATAGAGCACATCCTTTCTGGCTACAAAAATACTGTCCTGCTCATGAGCGTGATAGGACCCCTCGGTGTTGTAGCTGTGAAAGAATGGAGCCCCGAGACACAAGATATTTGTTACTTGACGATGGTCGGAAGCTGTGTCTGGAGTGTCTAGACTCAGCAATTATGGACACTCACGAGTGTCAACCCCTTTACCTTGAAATACAAGATTTTTATGAAGGTTTAAACATGAAAGTGAAGCAGCAAGTTCCAATGCTCTTGGTCGAGAGACAAGCTCTAAATGAGGCCATGGAGGGAGAAAAGAATGGTCATCGTCACATGCCGGAGACTAGAGGACTCTGCTTGTCAGAAGAACAGACCGTTACTACTATTCTGAGGAGGCCAAAGATTGGAGATGGCTACCGGATGATAGACATGGTAACTGAGCCTCATAGGTTGATTCGTCGCTGTGAAGTAACAGCAATTCTCGTGTTGTATGGCCTTCCTAGGTTGTTGACAGGGTCAATTCTGGCTCATGAGATGATGCACGCATGGCTAAGGCTTAACGGCTATCCAAACCTGAGCCCAGAGGTTGAAGAAGGTATCTGCCAAGTGCTAGCACATATGTGGCTGGACGCCGAGACATATTCTACACCAGGTAGTGATGTTGCCTCATCATCATCCTCTTCTTCCTCCTCCCCGTCCTCTTCTAATTCGGCATCATCTAAGAAGGGTAAACGCTCTGACTTTGAGAAAAAACTCGGTGAATTTTTCAAACACCAAATTGAGTCGGATTCATCAACAGCTTACGGAGAAGGGTTCAGGATAGGTAACCAAGCTGTGCTCAAGTTTGGTCTGAGGAGGACCCTCGACCATATTCGATTGATCGGAAATTTTCCCGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0032182 GO:0043130 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DA1-like LIM

Protein Analysis

542

Amino Acids

61.3

Weight (kDa)

5.34

Isoelectric Point (pI)

58.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LIM PF00412 172 - 227 2.8e-10 LIM domain
DA1-like PF12315 321 - 535 9.9e-103 Protein DA1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 1130
AciI CCGC 1 cut(s) 342
AcoI YGGCCR 3 cut(s) 34, 309, 538
AcsI RAATTY 4 cut(s) 347, 724, 1487, 1612
AcuI CTGAAG 1 cut(s) 267
AcyI GRCGYC 1 cut(s) 1356
AfaI GTAC 1 cut(s) 506
AfiI CCNNNNNNNGG 1 cut(s) 1130
AflIII ACRYGT 1 cut(s) 512
AgsI TTSAA 8 cut(s) 254, 287, 386, 422, 476, 941, 1316, 1495
AjnI CCWGG 2 cut(s) 448, 1375
AleI CACNNNNGTG 1 cut(s) 1345
AluBI AGCT 7 cut(s) 500, 731, 819, 877, 1016, 1526, 1559
AluI AGCT 7 cut(s) 500, 731, 819, 877, 1016, 1526, 1559
Alw21I GWGCWC 2 cut(s) 754, 1566
Alw26I GTCTC 4 cut(s) 834, 1003, 1061, 1355
Ama87I CYCGRG 2 cut(s) 837, 1619
AoxI GGCC 6 cut(s) 34, 309, 538, 1026, 1121, 1219
ApeKI GCWGC 3 cut(s) 522, 679, 982
ApoI RAATTY 4 cut(s) 347, 724, 1487, 1612
ArsI GACNNNNNNTTYG 2 cut(s) 763, 795
AspA2I CCTAGG 1 cut(s) 1226
AspS9I GGNCC 2 cut(s) 801, 1584
AsuHPI GGTGA 3 cut(s) 641, 677, 1496
AsuNHI GCTAGC 1 cut(s) 1336
AvaI CYCGRG 2 cut(s) 837, 1619
AvaII GGWCC 2 cut(s) 801, 1584
AvrII CCTAGG 1 cut(s) 1226
BalI TGGCCA 2 cut(s) 36, 540
BanII GRGCYC 2 cut(s) 837, 1307
BauI CACGAG 3 cut(s) 93, 917, 1208
BbsI GAAGAC 1 cut(s) 117
Bbv12I GWGCWC 2 cut(s) 754, 1566
BbvI GCAGC 3 cut(s) 509, 691, 994
BccI CCATC 2 cut(s) 860, 1130
BceAI ACGGC 2 cut(s) 582, 1303
BciT130I CCWGG 2 cut(s) 450, 1377
BclI TGATCA 1 cut(s) 432
BcoDI GTCTC 4 cut(s) 834, 1003, 1061, 1355
BfaI CTAG 5 cut(s) 149, 893, 1071, 1227, 1337
BisI GCNGC 3 cut(s) 523, 680, 983
BlnI CCTAGG 1 cut(s) 1226
BlsI GCNGC 3 cut(s) 524, 681, 984
Bme1390I CCNGG 2 cut(s) 450, 1377
Bme18I GGWCC 2 cut(s) 801, 1584
BmeT110I CYCGRG 2 cut(s) 837, 1619
BmgT120I GGNCC 2 cut(s) 801, 1584
BmiI GGNNCC 3 cut(s) 803, 834, 1586
BmrFI CCNGG 2 cut(s) 450, 1377
BmsI GCATC 5 cut(s) 289, 586, 691, 1254, 1448
BmtI GCTAGC 1 cut(s) 1340
BoxI GACNNNNGTC 1 cut(s) 867
BpiI GAAGAC 1 cut(s) 117
BplI GAGNNNNNCTC 2 cut(s) 1000, 1032
BpmI CTGGAG 1 cut(s) 905
Bpu10I CCTNAGC 2 cut(s) 1277, 1301
BpuEI CTTGAG 1 cut(s) 1550
BsaHI GRCGYC 1 cut(s) 1356
BsaJI CCNNGG 4 cut(s) 541, 806, 1029, 1226
BsaWI WCCGGW 1 cut(s) 1143
Bsc4I CCNNNNNNNGG 1 cut(s) 1130
Bse118I RCCGGY 1 cut(s) 208
BseBI CCWGG 2 cut(s) 450, 1377
BseDI CCNNGG 4 cut(s) 541, 806, 1029, 1226
BseGI GGATG 6 cut(s) 202, 577, 714, 754, 1152, 1400
BseLI CCNNNNNNNGG 1 cut(s) 1130
BseMII CTCAG 5 cut(s) 912, 1106, 1155, 1292, 1568
BseRI GAGGAG 4 cut(s) 435, 1132, 1405, 1594
BseXI GCAGC 3 cut(s) 509, 691, 994
BseYI CCCAGC 2 cut(s) 500, 731
Bsh1285I CGRYCG 1 cut(s) 308
BshFI GGCC 6 cut(s) 36, 311, 540, 1028, 1123, 1221
BsiEI CGRYCG 1 cut(s) 308
BsiHKAI GWGCWC 2 cut(s) 754, 1566
BsiHKCI CYCGRG 2 cut(s) 837, 1619
BsiSI CCGG 3 cut(s) 209, 1064, 1144
BslFI GGGAC 2 cut(s) 98, 121
BslI CCNNNNNNNGG 1 cut(s) 1130
BsmAI GTCTC 4 cut(s) 834, 1003, 1061, 1355
BsmFI GGGAC 2 cut(s) 98, 121
BsmI GAATGC 1 cut(s) 590
BsnI GGCC 6 cut(s) 36, 311, 540, 1028, 1123, 1221
BsoBI CYCGRG 2 cut(s) 837, 1619
Bsp1286I GDGCHC 4 cut(s) 754, 837, 1307, 1566
Bsp143I GATC 5 cut(s) 151, 272, 305, 432, 1605
Bsp19I CCATGG 2 cut(s) 541, 1029
BspACI CCGC 1 cut(s) 342
BspANI GGCC 6 cut(s) 36, 311, 540, 1028, 1123, 1221
BspCNI CTCAG 5 cut(s) 911, 1107, 1156, 1293, 1569
BspHI TCATGA 2 cut(s) 787, 1255
BspLI GGNNCC 3 cut(s) 803, 834, 1586
BspOI GCTAGC 1 cut(s) 1340
BsrFI RCCGGY 1 cut(s) 208
BssAI RCCGGY 1 cut(s) 208
BssECI CCNNGG 4 cut(s) 541, 806, 1029, 1226
BssMI GATC 5 cut(s) 151, 272, 305, 432, 1605
BssNI GRCGYC 1 cut(s) 1356
BssSI CACGAG 3 cut(s) 93, 917, 1208
BssT1I CCWWGG 3 cut(s) 541, 1029, 1226
Bst2BI CACGAG 3 cut(s) 93, 917, 1208
Bst2UI CCWGG 2 cut(s) 450, 1377
Bst4CI ACNGT 3 cut(s) 135, 779, 1102
Bst6I CTCTTC 2 cut(s) 1411, 1432
BstACI GRCGYC 1 cut(s) 1356
BstC8I GCNNGC 5 cut(s) 498, 520, 604, 1269, 1338
BstDEI CTNAG 8 cut(s) 15, 898, 1115, 1164, 1277, 1301, 1446, 1577
BstDSI CCRYGG 2 cut(s) 541, 1029
BstEII GGTNACC 1 cut(s) 1550
BstF5I GGATG 6 cut(s) 202, 577, 714, 754, 1152, 1400
BstKTI GATC 5 cut(s) 154, 275, 308, 435, 1608
BstMAI GTCTC 4 cut(s) 834, 1003, 1061, 1355
BstMBI GATC 5 cut(s) 151, 272, 305, 432, 1605
BstMCI CGRYCG 1 cut(s) 308
BstMWI GCNNNNNNNGC 5 cut(s) 308, 594, 603, 702, 1273
BstNI CCWGG 2 cut(s) 450, 1377
BstNSI RCATGY 2 cut(s) 516, 1063
BstPAI GACNNNNGTC 1 cut(s) 867
BstPI GGTNACC 1 cut(s) 1550
BstSCI CCNGG 2 cut(s) 448, 1375
BstV1I GCAGC 3 cut(s) 509, 691, 994
BstV2I GAAGAC 1 cut(s) 117
BsuRI GGCC 6 cut(s) 36, 311, 540, 1028, 1123, 1221
BtgI CCRYGG 2 cut(s) 541, 1029
BtgZI GCGATG 1 cut(s) 711
BtsCI GGATG 6 cut(s) 202, 577, 714, 754, 1152, 1400
BtsIMutI CAGTG 1 cut(s) 131
Cac8I GCNNGC 5 cut(s) 498, 520, 604, 1269, 1338
CciI TCATGA 2 cut(s) 787, 1255
Cfr10I RCCGGY 1 cut(s) 208
Cfr13I GGNCC 2 cut(s) 801, 1584
CseI GACGC 1 cut(s) 1364
CsiI ACCWGGT 1 cut(s) 1375
Csp6I GTAC 1 cut(s) 505
CviQI GTAC 1 cut(s) 505
DdeI CTNAG 8 cut(s) 15, 898, 1115, 1164, 1277, 1301, 1446, 1577
DpnI GATC 5 cut(s) 153, 274, 307, 434, 1607
DpnII GATC 5 cut(s) 151, 272, 305, 432, 1605
DraI TTTAAA 1 cut(s) 966
EaeI YGGCCR 3 cut(s) 34, 309, 538
Eam1104I CTCTTC 2 cut(s) 1411, 1432
EarI CTCTTC 2 cut(s) 1411, 1432
Eco130I CCWWGG 3 cut(s) 541, 1029, 1226
Eco24I GRGCYC 2 cut(s) 837, 1307
Eco47I GGWCC 2 cut(s) 801, 1584
Eco57I CTGAAG 1 cut(s) 267
Eco88I CYCGRG 2 cut(s) 837, 1619
Eco91I GGTNACC 1 cut(s) 1550
EcoO109I RGGNCCY 2 cut(s) 801, 1584
EcoO65I GGTNACC 1 cut(s) 1550
EcoRII CCWGG 2 cut(s) 448, 1375
EcoT14I CCWWGG 3 cut(s) 541, 1029, 1226
EcoT38I GRGCYC 2 cut(s) 837, 1307
ErhI CCWWGG 3 cut(s) 541, 1029, 1226
FaqI GGGAC 2 cut(s) 98, 121
FauI CCCGC 1 cut(s) 349
FauNDI CATATG 1 cut(s) 1344
FbaI TGATCA 1 cut(s) 432
Fnu4HI GCNGC 3 cut(s) 523, 680, 983
FokI GGATG 6 cut(s) 209, 564, 701, 741, 1159, 1387
FriOI GRGCYC 2 cut(s) 837, 1307
Fsp4HI GCNGC 3 cut(s) 523, 680, 983
FspBI CTAG 5 cut(s) 149, 893, 1071, 1227, 1337
GluI GCNGC 3 cut(s) 523, 680, 983
GsaI CCCAGC 2 cut(s) 504, 735
GsuI CTGGAG 1 cut(s) 905
HaeIII GGCC 6 cut(s) 36, 311, 540, 1028, 1123, 1221
HapII CCGG 3 cut(s) 209, 1064, 1144
HgaI GACGC 1 cut(s) 1364
Hin1I GRCGYC 1 cut(s) 1356
HincII GTYRAC 2 cut(s) 926, 1236
HindII GTYRAC 2 cut(s) 926, 1236
HpaII CCGG 3 cut(s) 209, 1064, 1144
HphI GGTGA 3 cut(s) 641, 677, 1496
Hpy166II GTNNAC 3 cut(s) 926, 1236, 1457
Hpy8I GTNNAC 3 cut(s) 926, 1236, 1457
Hpy99I CGWCG 1 cut(s) 1188
HpyAV CCTTC 5 cut(s) 953, 1232, 1313, 1444, 1529
HpyCH4III ACNGT 3 cut(s) 135, 779, 1102
HpyCH4V TGCA 7 cut(s) 131, 410, 525, 561, 606, 705, 1267
HpyF10VI GCNNNNNNNGC 5 cut(s) 308, 594, 603, 702, 1273
HpyF3I CTNAG 8 cut(s) 15, 898, 1115, 1164, 1277, 1301, 1446, 1577
Hsp92I GRCGYC 1 cut(s) 1356
Ksp22I TGATCA 1 cut(s) 432
Kzo9I GATC 5 cut(s) 151, 272, 305, 432, 1605
LmnI GCTCC 2 cut(s) 676, 832
Lsp1109I GCAGC 3 cut(s) 509, 691, 994
LweI GCATC 5 cut(s) 289, 586, 691, 1254, 1448
MabI ACCWGGT 1 cut(s) 1375
MaeI CTAG 5 cut(s) 149, 893, 1071, 1227, 1337
MaeIII GTNAC 6 cut(s) 855, 1055, 1102, 1159, 1195, 1550
MalI GATC 5 cut(s) 153, 274, 307, 434, 1607
MboI GATC 5 cut(s) 151, 272, 305, 432, 1605
MfeI CAATTG 1 cut(s) 386
MhlI GDGCHC 4 cut(s) 754, 837, 1307, 1566
MlsI TGGCCA 2 cut(s) 36, 540
MluNI TGGCCA 2 cut(s) 36, 540
MlyI GAGTC 3 cut(s) 890, 1071, 1517
MmeI TCCRAC 3 cut(s) 303, 851, 1491
Mox20I TGGCCA 2 cut(s) 36, 540
MscI TGGCCA 2 cut(s) 36, 540
MseI TTAA 3 cut(s) 24, 965, 1284
MslI CAYNNNNRTG 4 cut(s) 459, 792, 974, 1345
Msp20I TGGCCA 2 cut(s) 36, 540
MspA1I CMGCKG 2 cut(s) 500, 731
MspI CCGG 3 cut(s) 209, 1064, 1144
MspR9I CCNGG 2 cut(s) 450, 1377
MssI GTTTAAAC 1 cut(s) 966
MunI CAATTG 1 cut(s) 386
Mva1269I GAATGC 1 cut(s) 590
MvaI CCWGG 2 cut(s) 450, 1377
MwoI GCNNNNNNNGC 5 cut(s) 308, 594, 603, 702, 1273
NcoI CCATGG 2 cut(s) 541, 1029
NdeI CATATG 1 cut(s) 1344
NdeII GATC 5 cut(s) 151, 272, 305, 432, 1605
NheI GCTAGC 1 cut(s) 1336
NlaIV GGNNCC 3 cut(s) 803, 834, 1586
NmeAIII GCCGAG 1 cut(s) 1384
NmuCI GTSAC 1 cut(s) 1055
NspI RCATGY 2 cut(s) 516, 1063
OliI CACNNNNGTG 1 cut(s) 1345
PagI TCATGA 2 cut(s) 787, 1255
PciI ACATGT 1 cut(s) 512
PctI GAATGC 1 cut(s) 590
PfeI GAWTC 9 cut(s) 19, 98, 182, 221, 254, 287, 646, 1180, 1514
PflFI GACNNNGTC 1 cut(s) 1240
PflMI CCANNNNNTGG 1 cut(s) 1130
PfoI TCCNGGA 1 cut(s) 448
PkrI GCNGC 3 cut(s) 524, 681, 984
Ple19I CGATCG 1 cut(s) 308
PleI GAGTC 3 cut(s) 890, 1071, 1516
PmeI GTTTAAAC 1 cut(s) 966
PpsI GAGTC 3 cut(s) 890, 1071, 1516
PpuMI RGGWCCY 2 cut(s) 801, 1584
PscI ACATGT 1 cut(s) 512
PshAI GACNNNNGTC 1 cut(s) 867
Psp5II RGGWCCY 2 cut(s) 801, 1584
Psp6I CCWGG 2 cut(s) 448, 1375
PspEI GGTNACC 1 cut(s) 1550
PspFI CCCAGC 2 cut(s) 500, 731
PspGI CCWGG 2 cut(s) 448, 1375
PspN4I GGNNCC 3 cut(s) 803, 834, 1586
PspPI GGNCC 2 cut(s) 801, 1584
PspPPI RGGWCCY 2 cut(s) 801, 1584
PsyI GACNNNGTC 1 cut(s) 1240
PvuI CGATCG 1 cut(s) 308
PvuII CAGCTG 2 cut(s) 500, 731
RsaI GTAC 1 cut(s) 506
RsaNI GTAC 1 cut(s) 505
RseI CAYNNNNRTG 4 cut(s) 459, 792, 974, 1345
SaqAI TTAA 3 cut(s) 24, 965, 1284
SatI GCNGC 3 cut(s) 523, 680, 983
Sau3AI GATC 5 cut(s) 151, 272, 305, 432, 1605
Sau96I GGNCC 2 cut(s) 801, 1584
SchI GAGTC 3 cut(s) 890, 1071, 1517
ScrFI CCNGG 2 cut(s) 450, 1377
SduI GDGCHC 4 cut(s) 754, 837, 1307, 1566
SexAI ACCWGGT 1 cut(s) 1375
SfaNI GCATC 5 cut(s) 289, 586, 691, 1254, 1448
SinI GGWCC 2 cut(s) 801, 1584
SmiMI CAYNNNNRTG 4 cut(s) 459, 792, 974, 1345
SmlI CTYRAG 1 cut(s) 1565
SmoI CTYRAG 1 cut(s) 1565
SsiI CCGC 1 cut(s) 342
SspMI CTAG 5 cut(s) 149, 893, 1071, 1227, 1337
StyD4I CCNGG 2 cut(s) 448, 1375
StyI CCWWGG 3 cut(s) 541, 1029, 1226
TaaI ACNGT 3 cut(s) 135, 779, 1102
TaqI TCGA 5 cut(s) 174, 304, 1005, 1590, 1600
TfiI GAWTC 9 cut(s) 19, 98, 182, 221, 254, 287, 646, 1180, 1514
Tru1I TTAA 3 cut(s) 24, 965, 1284
Tru9I TTAA 3 cut(s) 24, 965, 1284
TscAI CASTG 1 cut(s) 138
TseFI GTSAC 1 cut(s) 1055
TseI GCWGC 3 cut(s) 522, 679, 982
Tsp45I GTSAC 1 cut(s) 1055
TspGWI ACGGA 2 cut(s) 581, 1545
TspRI CASTG 1 cut(s) 138
Tth111I GACNNNGTC 1 cut(s) 1240
Van91I CCANNNNNTGG 1 cut(s) 1130
VpaK11BI GGWCC 2 cut(s) 801, 1584
XapI RAATTY 4 cut(s) 347, 724, 1487, 1612
XbaI TCTAGA 1 cut(s) 892
XceI RCATGY 2 cut(s) 516, 1063
XmaJI CCTAGG 1 cut(s) 1226
XspI CTAG 5 cut(s) 149, 893, 1071, 1227, 1337
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.