pycom15g24430

WD40 repeats

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0008489
Physical Location & Seq
Forward (+)
18609538 .. 18610630
1093 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1011 bp
ATGGGTCTGGAAACACTTAACAAACAATTTGCCAGTTCCTCTGCAGCAGATGAACAGGCTAAAGCTGAAGAAGAATTCAAGAAAACAATGTACGGTGCAGCTGATGGCAGCAGCAGTGATGAAGAGGGAACTTCAAAAGCTAAAAAGTTACACATTCGAATAAGAGACAAGCCCATTGCTTCTACTGCTGTGGATGTGGATAAAATCAAAGAAGCCACGAAGCAGCTCAAACTTGGTGAAGGGTTGGGTCCACCTATGACCAGGACCAAATCATTGACCATGGGATCCCAGGACCTTAGCCAGATGTTATCCCAACCACGTCCTCCAGTTAACAGTGGGTCAATGGCTCCTCGTGTAGGCTCTGCCCCTGGTGATCTATTTGGTATGGACTCGTTTACACAACCTGCAACAGTATCACATCAAGCTCCTACCTCAACAGTTAAGGGGGTTGGAGCTGTACCTATTCCAGAGGACTTTTTCCAGAATACGATACCATCCCTTCAGGTTGCAGCCACACTGCCCCCTCCTGGGACCTATCTTTCAAAAATGGATCAAGCTTCTCAAGGGTTTGAAAGCAACAAGAAAGCATTTAACCAGACCAATGCATCCAGTGCCAATGTCCGTCTTCCTGATGCTGGTGTTCCCCCTCAAGCTAGTCAACTGGCTGCTCCATTCGAGCCCGTTGGACTTCCTGATGGTGGTGTCCCACCATCCTTAGGCCAGGTTGCTGCGCAGCAACAATCTCATATTCAATCAACTCAGTTCCCAGTTTCTACTCGACCTCTTGATCTTAGTGTCCTTGGGGTTCCAAATTCTACTGACTCTGGAAAACCTTCAGTGCAGCCTCCTTCTCCACCATCTTCTGTGCGTCCTGGACAGGTACCCCGTGGTGCTGCAGCTTCTGTATGTTTTAAAACTGGAGTTGCCCACCTTGAGCAGAATCAACTTTCAGATGCGTTGTCTTGCTTTGACGAAGCTTTTCTTGCATTGGCTAAGGATCGTCGTTATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
COPI_C

Protein Analysis

337

Amino Acids

35.18

Weight (kDa)

6.45

Isoelectric Point (pI)

49.64

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 732
Acc36I ACCTGC 1 cut(s) 412
Acc65I GGTACC 1 cut(s) 880
AccB1I GGYRCC 1 cut(s) 880
AclWI GGATC 4 cut(s) 279, 292, 558, 1005
AcsI RAATTY 2 cut(s) 74, 811
AcuI CTGAAG 3 cut(s) 87, 485, 819
AfaI GTAC 3 cut(s) 92, 459, 882
AfiI CCNNNNNNNGG 6 cut(s) 356, 527, 528, 635, 698, 716
AgsI TTSAA 5 cut(s) 79, 135, 543, 572, 752
AjiI CACGTC 1 cut(s) 320
AjnI CCWGG 6 cut(s) 260, 288, 367, 526, 720, 871
Alw26I GTCTC 1 cut(s) 159
AlwI GGATC 4 cut(s) 279, 292, 558, 1005
AlwNI CAGNNNCTG 1 cut(s) 902
AoxI GGCC 1 cut(s) 718
ApoI RAATTY 2 cut(s) 74, 811
Asp700I GAANNNNTTC 2 cut(s) 832, 978
Asp718I GGTACC 1 cut(s) 880
AspLEI GCGC 1 cut(s) 733
AspS9I GGNCC 4 cut(s) 248, 264, 292, 531
AsuHPI GGTGA 2 cut(s) 248, 383
AsuII TTCGAA 1 cut(s) 157
AvaII GGWCC 4 cut(s) 248, 264, 292, 531
AxyI CCTNAGG 1 cut(s) 715
BamHI GGATCC 1 cut(s) 284
BanI GGYRCC 1 cut(s) 880
BanII GRGCYC 1 cut(s) 681
BauI CACGAG 1 cut(s) 351
BbsI GAAGAC 1 cut(s) 617
BccI CCATC 5 cut(s) 98, 502, 689, 718, 865
BciT130I CCWGG 6 cut(s) 262, 290, 369, 528, 722, 873
BcoDI GTCTC 1 cut(s) 159
BfaI CTAG 1 cut(s) 654
BfmI CTRYAG 2 cut(s) 42, 894
BfuAI ACCTGC 1 cut(s) 412
Bme1390I CCNGG 6 cut(s) 262, 290, 369, 528, 722, 873
Bme18I GGWCC 4 cut(s) 248, 264, 292, 531
BmgBI CACGTC 1 cut(s) 320
BmgT120I GGNCC 4 cut(s) 248, 264, 292, 531
BmiI GGNNCC 6 cut(s) 249, 286, 348, 532, 807, 882
BmrFI CCNGG 6 cut(s) 262, 290, 369, 528, 722, 873
BmrI ACTGGG 1 cut(s) 761
BmsI GCATC 3 cut(s) 614, 622, 943
BmuI ACTGGG 1 cut(s) 761
BpiI GAAGAC 1 cut(s) 617
BpmI CTGGAG 2 cut(s) 309, 939
Bpu10I CCTNAGC 2 cut(s) 296, 993
Bpu14I TTCGAA 1 cut(s) 157
BpuEI CTTGAG 3 cut(s) 546, 633, 953
BsaJI CCNNGG 6 cut(s) 279, 288, 367, 527, 799, 886
Bsc4I CCNNNNNNNGG 6 cut(s) 356, 527, 528, 635, 698, 716
Bse1I ACTGG 6 cut(s) 33, 326, 609, 666, 767, 922
Bse21I CCTNAGG 1 cut(s) 715
Bse3DI GCAATG 1 cut(s) 174
BseBI CCWGG 6 cut(s) 262, 290, 369, 528, 722, 873
BseDI CCNNGG 6 cut(s) 279, 288, 367, 527, 799, 886
BseGI GGATG 4 cut(s) 199, 494, 605, 710
BseLI CCNNNNNNNGG 6 cut(s) 356, 527, 528, 635, 698, 716
BseMI GCAATG 1 cut(s) 174
BseMII CTCAG 1 cut(s) 773
BseNI ACTGG 6 cut(s) 33, 326, 609, 666, 767, 922
BseRI GAGGAG 1 cut(s) 339
BsgI GTGCAG 2 cut(s) 117, 860
BshFI GGCC 1 cut(s) 720
BshNI GGYRCC 1 cut(s) 880
BslFI GGGAC 2 cut(s) 544, 689
BslI CCNNNNNNNGG 6 cut(s) 356, 527, 528, 635, 698, 716
BsmAI GTCTC 1 cut(s) 159
BsmFI GGGAC 2 cut(s) 544, 689
BsnI GGCC 1 cut(s) 720
Bsp119I TTCGAA 1 cut(s) 157
Bsp1286I GDGCHC 1 cut(s) 681
Bsp143I GATC 5 cut(s) 284, 373, 550, 787, 997
Bsp19I CCATGG 1 cut(s) 279
BspANI GGCC 1 cut(s) 720
BspCNI CTCAG 1 cut(s) 772
BspLI GGNNCC 6 cut(s) 249, 286, 348, 532, 807, 882
BspMAI CTGCAG 2 cut(s) 46, 898
BspMI ACCTGC 1 cut(s) 412
BspPI GGATC 4 cut(s) 279, 292, 558, 1005
BspT104I TTCGAA 1 cut(s) 157
BspT107I GGYRCC 1 cut(s) 880
BsrDI GCAATG 1 cut(s) 174
BsrI ACTGG 6 cut(s) 33, 326, 609, 666, 767, 922
BssECI CCNNGG 6 cut(s) 279, 288, 367, 527, 799, 886
BssMI GATC 5 cut(s) 284, 373, 550, 787, 997
BssSI CACGAG 1 cut(s) 351
BssT1I CCWWGG 2 cut(s) 279, 799
Bst2BI CACGAG 1 cut(s) 351
Bst2UI CCWGG 6 cut(s) 262, 290, 369, 528, 722, 873
Bst4CI ACNGT 4 cut(s) 95, 335, 412, 439
Bst6I CTCTTC 1 cut(s) 117
BstAPI GCANNNNNTGC 1 cut(s) 611
BstBI TTCGAA 1 cut(s) 157
BstDEI CTNAG 5 cut(s) 296, 715, 759, 791, 993
BstDSI CCRYGG 2 cut(s) 279, 886
BstENI CCTNNNNNAGG 1 cut(s) 354
BstF5I GGATG 4 cut(s) 199, 494, 605, 710
BstHHI GCGC 1 cut(s) 733
BstKTI GATC 5 cut(s) 287, 376, 553, 790, 1000
BstMAI GTCTC 1 cut(s) 159
BstMBI GATC 5 cut(s) 284, 373, 550, 787, 997
BstMWI GCNNNNNNNGC 3 cut(s) 185, 611, 983
BstNI CCWGG 6 cut(s) 262, 290, 369, 528, 722, 873
BstSCI CCNGG 6 cut(s) 260, 288, 367, 526, 720, 871
BstSFI CTRYAG 2 cut(s) 42, 894
BstV2I GAAGAC 1 cut(s) 617
BstX2I RGATCY 1 cut(s) 284
BstYI RGATCY 1 cut(s) 284
Bsu36I CCTNAGG 1 cut(s) 715
BsuRI GGCC 1 cut(s) 720
BtgI CCRYGG 2 cut(s) 279, 886
BtrI CACGTC 1 cut(s) 320
BtsCI GGATG 4 cut(s) 199, 494, 605, 710
BtsI GCAGTG 2 cut(s) 121, 515
BtsIMutI CAGTG 5 cut(s) 121, 340, 515, 616, 843
BveI ACCTGC 1 cut(s) 412
CaiI CAGNNNCTG 1 cut(s) 902
CfoI GCGC 1 cut(s) 733
Cfr13I GGNCC 4 cut(s) 248, 264, 292, 531
CseI GACGC 1 cut(s) 857
Csp6I GTAC 3 cut(s) 91, 458, 881
CviAII CATG 1 cut(s) 280
CviQI GTAC 3 cut(s) 91, 458, 881
DdeI CTNAG 5 cut(s) 296, 715, 759, 791, 993
DpnI GATC 5 cut(s) 286, 375, 552, 789, 999
DpnII GATC 5 cut(s) 284, 373, 550, 787, 997
DraI TTTAAA 1 cut(s) 913
Eam1104I CTCTTC 1 cut(s) 117
EarI CTCTTC 1 cut(s) 117
Eco130I CCWWGG 2 cut(s) 279, 799
Eco24I GRGCYC 1 cut(s) 681
Eco47I GGWCC 4 cut(s) 248, 264, 292, 531
Eco57I CTGAAG 3 cut(s) 87, 485, 819
Eco81I CCTNAGG 1 cut(s) 715
EcoNI CCTNNNNNAGG 1 cut(s) 354
EcoO109I RGGNCCY 2 cut(s) 292, 531
EcoRI GAATTC 1 cut(s) 74
EcoRII CCWGG 6 cut(s) 260, 288, 367, 526, 720, 871
EcoT14I CCWWGG 2 cut(s) 279, 799
EcoT22I ATGCAT 1 cut(s) 607
EcoT38I GRGCYC 1 cut(s) 681
ErhI CCWWGG 2 cut(s) 279, 799
FaeI CATG 1 cut(s) 283
FaiI YATR 5 cut(s) 257, 281, 386, 747, 907
FalI AAGNNNNNCTT 2 cut(s) 966, 998
FaqI GGGAC 2 cut(s) 544, 689
FatI CATG 1 cut(s) 279
FokI GGATG 4 cut(s) 206, 481, 592, 697
FriOI GRGCYC 1 cut(s) 681
FspBI CTAG 1 cut(s) 654
FspI TGCGCA 1 cut(s) 732
GlaI GCGC 1 cut(s) 732
GsuI CTGGAG 2 cut(s) 309, 939
HaeIII GGCC 1 cut(s) 720
HgaI GACGC 1 cut(s) 857
HhaI GCGC 1 cut(s) 733
Hin1II CATG 1 cut(s) 283
Hin6I GCGC 1 cut(s) 731
HinP1I GCGC 1 cut(s) 731
HincII GTYRAC 2 cut(s) 331, 659
HindII GTYRAC 2 cut(s) 331, 659
HindIII AAGCTT 2 cut(s) 555, 975
HinfI GANTC 3 cut(s) 389, 821, 940
HpaI GTTAAC 1 cut(s) 331
HphI GGTGA 2 cut(s) 248, 383
Hpy166II GTNNAC 4 cut(s) 251, 331, 396, 659
Hpy188I TCNGA 1 cut(s) 952
Hpy188III TCNNGA 8 cut(s) 8, 79, 467, 481, 629, 692, 785, 825
Hpy8I GTNNAC 4 cut(s) 251, 331, 396, 659
Hpy99I CGWCG 1 cut(s) 1005
HpyAV CCTTC 4 cut(s) 233, 509, 843, 858
HpyCH4III ACNGT 4 cut(s) 95, 335, 412, 439
HpyCH4IV ACGT 1 cut(s) 319
HpyCH4V TGCA 8 cut(s) 44, 98, 407, 509, 605, 841, 896, 986
HpyF10VI GCNNNNNNNGC 3 cut(s) 185, 611, 983
HpyF3I CTNAG 5 cut(s) 296, 715, 759, 791, 993
HpySE526I ACGT 1 cut(s) 319
Hsp92II CATG 1 cut(s) 283
HspAI GCGC 1 cut(s) 731
KpnI GGTACC 1 cut(s) 884
KspAI GTTAAC 1 cut(s) 331
Kzo9I GATC 5 cut(s) 284, 373, 550, 787, 997
LmnI GCTCC 4 cut(s) 352, 430, 452, 673
LweI GCATC 3 cut(s) 614, 622, 943
MaeI CTAG 1 cut(s) 654
MaeII ACGT 1 cut(s) 319
MaeIII GTNAC 1 cut(s) 147
MalI GATC 5 cut(s) 286, 375, 552, 789, 999
MboI GATC 5 cut(s) 284, 373, 550, 787, 997
MboII GAAGA 5 cut(s) 80, 83, 134, 617, 852
MflI RGATCY 1 cut(s) 284
MhlI GDGCHC 1 cut(s) 681
MluCI AATT 3 cut(s) 26, 74, 811
MlyI GAGTC 2 cut(s) 383, 815
MmeI TCCRAC 2 cut(s) 430, 664
Mph1103I ATGCAT 1 cut(s) 607
MroXI GAANNNNTTC 2 cut(s) 832, 978
MseI TTAA 5 cut(s) 18, 330, 441, 591, 912
MspA1I CMGCKG 1 cut(s) 101
MspR9I CCNGG 6 cut(s) 262, 290, 369, 528, 722, 873
MvaI CCWGG 6 cut(s) 262, 290, 369, 528, 722, 873
MwoI GCNNNNNNNGC 3 cut(s) 185, 611, 983
NcoI CCATGG 1 cut(s) 279
NdeII GATC 5 cut(s) 284, 373, 550, 787, 997
NlaIII CATG 1 cut(s) 283
NlaIV GGNNCC 6 cut(s) 249, 286, 348, 532, 807, 882
NsbI TGCGCA 1 cut(s) 732
NsiI ATGCAT 1 cut(s) 607
NspV TTCGAA 1 cut(s) 157
PdmI GAANNNNTTC 2 cut(s) 832, 978
PfeI GAWTC 1 cut(s) 940
PfoI TCCNGGA 1 cut(s) 871
PleI GAGTC 2 cut(s) 383, 815
PpsI GAGTC 2 cut(s) 383, 815
PpuMI RGGWCCY 2 cut(s) 292, 531
Psp5II RGGWCCY 2 cut(s) 292, 531
Psp6I CCWGG 6 cut(s) 260, 288, 367, 526, 720, 871
PspGI CCWGG 6 cut(s) 260, 288, 367, 526, 720, 871
PspN4I GGNNCC 6 cut(s) 249, 286, 348, 532, 807, 882
PspPI GGNCC 4 cut(s) 248, 264, 292, 531
PspPPI RGGWCCY 2 cut(s) 292, 531
PstI CTGCAG 2 cut(s) 46, 898
PstNI CAGNNNCTG 1 cut(s) 902
PsuI RGATCY 1 cut(s) 284
PvuII CAGCTG 1 cut(s) 101
RsaI GTAC 3 cut(s) 92, 459, 882
RsaNI GTAC 3 cut(s) 91, 458, 881
SaqAI TTAA 5 cut(s) 18, 330, 441, 591, 912
Sau3AI GATC 5 cut(s) 284, 373, 550, 787, 997
Sau96I GGNCC 4 cut(s) 248, 264, 292, 531
SchI GAGTC 2 cut(s) 383, 815
ScrFI CCNGG 6 cut(s) 262, 290, 369, 528, 722, 873
SduI GDGCHC 1 cut(s) 681
SfaNI GCATC 3 cut(s) 614, 622, 943
SfcI CTRYAG 2 cut(s) 42, 894
SfuI TTCGAA 1 cut(s) 157
SinI GGWCC 4 cut(s) 248, 264, 292, 531
SmlI CTYRAG 3 cut(s) 561, 648, 932
SmoI CTYRAG 3 cut(s) 561, 648, 932
Sse9I AATT 3 cut(s) 26, 74, 811
SspMI CTAG 1 cut(s) 654
StyD4I CCNGG 6 cut(s) 260, 288, 367, 526, 720, 871
StyI CCWWGG 2 cut(s) 279, 799
TaaI ACNGT 4 cut(s) 95, 335, 412, 439
TaiI ACGT 1 cut(s) 322
TaqI TCGA 3 cut(s) 157, 675, 778
TasI AATT 3 cut(s) 26, 74, 811
TfiI GAWTC 1 cut(s) 940
Tru1I TTAA 5 cut(s) 18, 330, 441, 591, 912
Tru9I TTAA 5 cut(s) 18, 330, 441, 591, 912
TscAI CASTG 5 cut(s) 121, 340, 522, 616, 843
TspDTI ATGAA 2 cut(s) 66, 135
TspGWI ACGGA 1 cut(s) 611
TspRI CASTG 5 cut(s) 121, 340, 522, 616, 843
VpaK11BI GGWCC 4 cut(s) 248, 264, 292, 531
XagI CCTNNNNNAGG 1 cut(s) 354
XapI RAATTY 2 cut(s) 74, 811
XmnI GAANNNNTTC 2 cut(s) 832, 978
XspI CTAG 1 cut(s) 654
Zsp2I ATGCAT 1 cut(s) 607
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.