pycom15g24670

heat shock factor protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0013192
Physical Location & Seq
Reverse (-)
18894790 .. 18896581
1792 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 747 bp
ATGTTACCTAAATATTTCAAGCACAACAATTTCTCAAGCTTCGTCCGCCAGCTTAATACCTATGGATTTCGAAAGACCGTGCCGGACAAATGGGAGTTCGCGAACGACAACTTCCAGCGAGGGCAAAAGGAGCTCCTGTCCCAGATCCGCCGCCGGAAATCTGTGACGTCAACGGCGGCGCACGCACCCCCCGGCGAAAAATCCGGAGTACCATCGACACCGTCGAACTCGGGGGAGGAGATGGCGTCGACGTCGACGTCGTCGCCGGACTCGAAGAACCCGGGGTCGGTGGAGATGGCGGCGCCGAGCCAGGTGTCGGATTTATCGGACGAGAACGAGAAGCTGAAGCGGGAGAACGAGAATCTGAACTCGGAGCTGGCGCAGACGAAGAAGCAGTGCGACGAGCTGGTGGGGTTTCTGGTGGAGCACATGAAGATGAAGCCCGATCAGATCAACCGCATCATTGGGCAGGGAATCTGTGGGCCTAGCGGCGATGATTTTGAAGCGGACGATCATGGTCTTGATCTGGATATGGAGGGTGGCGATGATGAGGAAGAAGAGGAGGAGGGATTGAAGCTGTTTGGGGTTTGGATGAAAGGGGAGGAGAAAATGAAGGGGAAGAAAACGGGGCGATATGAGAAATTGGGGGTTGGTGGTGGCGGCGCCGGCGGGCCCCGCGGGAAGAGAATGAAGGGAGGGGTGGAATTTCACGCGCCGATGATGAGGACCGGGAAGGTGTGCAACTAG

Protein Analysis

249

Amino Acids

27.29

Weight (kDa)

5.85

Isoelectric Point (pI)

36.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSF_DNA-bind PF00447 1 - 52 4.6e-17 HSF-type DNA-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013192)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G36990
fragaria_vesca FvH4_1g16030
malus_domestica MD02G1171800.v1.1 MD15G1283700.v1.1
prunus_persica Prupe.7G133600_v2.0.a1
pyrus_communis pycom02g13850 pycom15g24670
rosa_chinensis RchiOBHm_Chr2g0105601
rosa_laevigata RLG00000017444
rosa_multiflora Rmu_co8474487.1_g000001 Rmu_sc0001051.1_g000029
rosa_roxburghii Rroxscaffold_2G00138040
rosa_rugosa Rorug02G0129000
rosa_samantha Rh2AG180500 Rh2BG189300 Rh2CG184700 Rh2DG186200
rosa_wichuraiana Rw2G014110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 256
AatII GACGTC 3 cut(s) 170, 254, 260
AccB1I GGYRCC 2 cut(s) 301, 662
AccI GTMKAC 2 cut(s) 248, 254
AccII CGCG 3 cut(s) 101, 678, 713
AccIII TCCGGA 1 cut(s) 203
AclWI GGATC 1 cut(s) 139
AcsI RAATTY 1 cut(s) 704
AcuI CTGAAG 1 cut(s) 365
AcyI GRCGYC 6 cut(s) 167, 245, 251, 257, 302, 663
AfaI GTAC 1 cut(s) 210
AfiI CCNNNNNNNGG 2 cut(s) 286, 316
AgsI TTSAA 3 cut(s) 19, 503, 574
AjnI CCWGG 1 cut(s) 309
AluBI AGCT 7 cut(s) 39, 52, 133, 343, 376, 406, 577
AluI AGCT 7 cut(s) 39, 52, 133, 343, 376, 406, 577
Alw21I GWGCWC 2 cut(s) 135, 429
AlwI GGATC 1 cut(s) 139
Ama87I CYCGRG 2 cut(s) 229, 280
Aor13HI TCCGGA 1 cut(s) 203
AoxI GGCC 2 cut(s) 482, 671
ApaI GGGCCC 1 cut(s) 675
ApoI RAATTY 1 cut(s) 704
AspLEI GCGC 5 cut(s) 181, 304, 382, 665, 715
AspS9I GGNCC 4 cut(s) 482, 671, 672, 726
AsuC2I CCSGG 4 cut(s) 192, 281, 282, 730
AsuII TTCGAA 1 cut(s) 70
AvaI CYCGRG 2 cut(s) 229, 280
AvaII GGWCC 1 cut(s) 726
BaeGI GKGCMC 1 cut(s) 675
BanI GGYRCC 2 cut(s) 301, 662
BanII GRGCYC 2 cut(s) 135, 675
Bbv12I GWGCWC 2 cut(s) 135, 429
BccI CCATC 3 cut(s) 220, 235, 289
BceAI ACGGC 1 cut(s) 189
BciT130I CCWGG 1 cut(s) 311
BcnI CCSGG 4 cut(s) 192, 281, 282, 730
BfaI CTAG 2 cut(s) 486, 745
BfoI RGCGCY 2 cut(s) 305, 666
BisI GCNGC 5 cut(s) 151, 177, 300, 490, 661
BlsI GCNGC 5 cut(s) 152, 178, 301, 491, 662
Bme1390I CCNGG 5 cut(s) 192, 281, 282, 311, 730
Bme18I GGWCC 1 cut(s) 726
BmeT110I CYCGRG 2 cut(s) 229, 280
BmgT120I GGNCC 4 cut(s) 482, 671, 672, 726
BmiI GGNNCC 4 cut(s) 303, 664, 673, 674
BmrFI CCNGG 5 cut(s) 192, 281, 282, 311, 730
BmsI GCATC 1 cut(s) 468
Bpu14I TTCGAA 1 cut(s) 70
BpuEI CTTGAG 1 cut(s) 19
BpuMI CCSGG 4 cut(s) 192, 281, 282, 730
BsaHI GRCGYC 6 cut(s) 167, 245, 251, 257, 302, 663
BsaJI CCNNGG 4 cut(s) 190, 280, 281, 676
BsaWI WCCGGW 1 cut(s) 203
BsaXI ACNNNNNCTCC 4 cut(s) 122, 152, 230, 260
Bsc4I CCNNNNNNNGG 2 cut(s) 286, 316
Bse118I RCCGGY 1 cut(s) 665
BseAI TCCGGA 1 cut(s) 203
BseBI CCWGG 1 cut(s) 311
BseDI CCNNGG 4 cut(s) 190, 280, 281, 676
BseGI GGATG 1 cut(s) 597
BseLI CCNNNNNNNGG 2 cut(s) 286, 316
BseRI GAGGAG 4 cut(s) 251, 575, 578, 617
BseSI GKGCMC 1 cut(s) 675
Bsh1236I CGCG 3 cut(s) 101, 678, 713
BshFI GGCC 2 cut(s) 484, 673
BshNI GGYRCC 2 cut(s) 301, 662
BsiHKAI GWGCWC 2 cut(s) 135, 429
BsiHKCI CYCGRG 2 cut(s) 229, 280
BsiSI CCGG 8 cut(s) 83, 154, 192, 204, 266, 281, 666, 729
BslFI GGGAC 1 cut(s) 124
BslI CCNNNNNNNGG 2 cut(s) 286, 316
BsmFI GGGAC 1 cut(s) 124
BsnI GGCC 2 cut(s) 484, 673
BsoBI CYCGRG 2 cut(s) 229, 280
Bsp119I TTCGAA 1 cut(s) 70
Bsp120I GGGCCC 1 cut(s) 671
Bsp1286I GDGCHC 3 cut(s) 135, 429, 675
Bsp13I TCCGGA 1 cut(s) 203
Bsp143I GATC 5 cut(s) 144, 445, 450, 511, 523
Bsp68I TCGCGA 1 cut(s) 101
BspANI GGCC 2 cut(s) 484, 673
BspEI TCCGGA 1 cut(s) 203
BspFNI CGCG 3 cut(s) 101, 678, 713
BspLI GGNNCC 4 cut(s) 303, 664, 673, 674
BspPI GGATC 1 cut(s) 139
BspT104I TTCGAA 1 cut(s) 70
BspT107I GGYRCC 2 cut(s) 301, 662
BsrFI RCCGGY 1 cut(s) 665
BssAI RCCGGY 1 cut(s) 665
BssECI CCNNGG 4 cut(s) 190, 280, 281, 676
BssMI GATC 5 cut(s) 144, 445, 450, 511, 523
BssNI GRCGYC 6 cut(s) 167, 245, 251, 257, 302, 663
Bst2UI CCWGG 1 cut(s) 311
Bst4CI ACNGT 2 cut(s) 79, 222
Bst6I CTCTTC 2 cut(s) 552, 677
BstACI GRCGYC 6 cut(s) 167, 245, 251, 257, 302, 663
BstBI TTCGAA 1 cut(s) 70
BstC8I GCNNGC 5 cut(s) 50, 183, 378, 667, 671
BstDSI CCRYGG 1 cut(s) 676
BstF5I GGATG 1 cut(s) 597
BstFNI CGCG 3 cut(s) 101, 678, 713
BstH2I RGCGCY 2 cut(s) 305, 666
BstHHI GCGC 5 cut(s) 181, 304, 382, 665, 715
BstKTI GATC 5 cut(s) 147, 448, 453, 514, 526
BstMBI GATC 5 cut(s) 144, 445, 450, 511, 523
BstMWI GCNNNNNNNGC 5 cut(s) 45, 130, 182, 666, 675
BstNI CCWGG 1 cut(s) 311
BstSCI CCNGG 5 cut(s) 190, 279, 280, 309, 728
BstSLI GKGCMC 1 cut(s) 675
BstUI CGCG 3 cut(s) 101, 678, 713
BstX2I RGATCY 1 cut(s) 144
BstYI RGATCY 1 cut(s) 144
BsuRI GGCC 2 cut(s) 484, 673
BtgI CCRYGG 1 cut(s) 676
BtgZI GCGATG 2 cut(s) 507, 558
BtsCI GGATG 1 cut(s) 597
BtsI GCAGTG 1 cut(s) 401
BtsIMutI CAGTG 1 cut(s) 401
BtuMI TCGCGA 1 cut(s) 101
Cac8I GCNNGC 5 cut(s) 50, 183, 378, 667, 671
CfoI GCGC 5 cut(s) 181, 304, 382, 665, 715
Cfr10I RCCGGY 1 cut(s) 665
Cfr13I GGNCC 4 cut(s) 482, 671, 672, 726
Cfr42I CCGCGG 1 cut(s) 679
Cfr9I CCCGGG 1 cut(s) 280
CseI GACGC 1 cut(s) 234
Csp6I GTAC 1 cut(s) 209
CviAII CATG 2 cut(s) 430, 515
CviQI GTAC 1 cut(s) 209
DinI GGCGCC 2 cut(s) 303, 664
DpnI GATC 5 cut(s) 146, 447, 452, 513, 525
DpnII GATC 5 cut(s) 144, 445, 450, 511, 523
DrdI GACNNNNNNGTC 1 cut(s) 256
DseDI GACNNNNNNGTC 1 cut(s) 256
Eam1104I CTCTTC 2 cut(s) 552, 677
EarI CTCTTC 2 cut(s) 552, 677
EciI GGCGGA 2 cut(s) 35, 137
Ecl136II GAGCTC 1 cut(s) 133
Eco24I GRGCYC 2 cut(s) 135, 675
Eco47I GGWCC 1 cut(s) 726
Eco53kI GAGCTC 1 cut(s) 133
Eco57I CTGAAG 1 cut(s) 365
Eco88I CYCGRG 2 cut(s) 229, 280
EcoICRI GAGCTC 1 cut(s) 133
EcoO109I RGGNCCY 1 cut(s) 672
EcoRII CCWGG 1 cut(s) 309
EcoT38I GRGCYC 2 cut(s) 135, 675
EgeI GGCGCC 2 cut(s) 303, 664
EheI GGCGCC 2 cut(s) 303, 664
FaeI CATG 2 cut(s) 433, 518
FaiI YATR 5 cut(s) 63, 431, 516, 533, 636
FaqI GGGAC 1 cut(s) 124
FatI CATG 2 cut(s) 429, 514
FauI CCCGC 4 cut(s) 342, 662, 671, 683
FblI GTMKAC 2 cut(s) 248, 254
Fnu4HI GCNGC 5 cut(s) 151, 177, 300, 490, 661
FokI GGATG 1 cut(s) 604
FriOI GRGCYC 2 cut(s) 135, 675
Fsp4HI GCNGC 5 cut(s) 151, 177, 300, 490, 661
FspBI CTAG 2 cut(s) 486, 745
GlaI GCGC 5 cut(s) 180, 303, 381, 664, 714
GluI GCNGC 5 cut(s) 151, 177, 300, 490, 661
HaeII RGCGCY 2 cut(s) 305, 666
HaeIII GGCC 2 cut(s) 484, 673
HapII CCGG 8 cut(s) 83, 154, 192, 204, 266, 281, 666, 729
HgaI GACGC 1 cut(s) 234
HhaI GCGC 5 cut(s) 181, 304, 382, 665, 715
Hin1I GRCGYC 6 cut(s) 167, 245, 251, 257, 302, 663
Hin1II CATG 2 cut(s) 433, 518
Hin6I GCGC 5 cut(s) 179, 302, 380, 663, 713
HinP1I GCGC 5 cut(s) 179, 302, 380, 663, 713
HincII GTYRAC 3 cut(s) 171, 249, 255
HindII GTYRAC 3 cut(s) 171, 249, 255
HindIII AAGCTT 1 cut(s) 37
HinfI GANTC 3 cut(s) 269, 361, 474
HpaII CCGG 8 cut(s) 83, 154, 192, 204, 266, 281, 666, 729
Hpy166II GTNNAC 3 cut(s) 171, 249, 255
Hpy188I TCNGA 5 cut(s) 319, 328, 366, 373, 450
Hpy188III TCNNGA 4 cut(s) 100, 204, 521, 527
Hpy8I GTNNAC 3 cut(s) 171, 249, 255
Hpy99I CGWCG 8 cut(s) 226, 250, 253, 256, 259, 262, 265, 404
HpyAV CCTTC 3 cut(s) 607, 685, 727
HpyCH4III ACNGT 2 cut(s) 79, 222
HpyCH4IV ACGT 3 cut(s) 167, 251, 257
HpyCH4V TGCA 1 cut(s) 741
HpyF10VI GCNNNNNNNGC 5 cut(s) 45, 130, 182, 666, 675
HpySE526I ACGT 3 cut(s) 167, 251, 257
Hsp92I GRCGYC 6 cut(s) 167, 245, 251, 257, 302, 663
Hsp92II CATG 2 cut(s) 433, 518
HspAI GCGC 5 cut(s) 179, 302, 380, 663, 713
KasI GGCGCC 2 cut(s) 301, 662
Kpn2I TCCGGA 1 cut(s) 203
KroI GCCGGC 1 cut(s) 665
KroNI GCCGGC 1 cut(s) 667
KspI CCGCGG 1 cut(s) 679
Kzo9I GATC 5 cut(s) 144, 445, 450, 511, 523
LmnI GCTCC 4 cut(s) 130, 138, 373, 424
LweI GCATC 1 cut(s) 468
MaeI CTAG 2 cut(s) 486, 745
MaeII ACGT 3 cut(s) 167, 251, 257
MaeIII GTNAC 2 cut(s) 3, 163
MalI GATC 5 cut(s) 146, 447, 452, 513, 525
MboI GATC 5 cut(s) 144, 445, 450, 511, 523
MboII GAAGA 7 cut(s) 286, 400, 445, 566, 569, 631, 694
MflI RGATCY 1 cut(s) 144
MhlI GDGCHC 3 cut(s) 135, 429, 675
MluCI AATT 3 cut(s) 28, 641, 704
Mly113I GGCGCC 2 cut(s) 302, 663
MlyI GAGTC 1 cut(s) 263
MmeI TCCRAC 1 cut(s) 297
MreI CGCCGGCG 1 cut(s) 665
MroI TCCGGA 1 cut(s) 203
MroNI GCCGGC 1 cut(s) 665
MseI TTAA 1 cut(s) 54
MslI CAYNNNNRTG 1 cut(s) 434
MspA1I CMGCKG 1 cut(s) 678
MspI CCGG 8 cut(s) 83, 154, 192, 204, 266, 281, 666, 729
MspR9I CCNGG 5 cut(s) 192, 281, 282, 311, 730
MvaI CCWGG 1 cut(s) 311
MvnI CGCG 3 cut(s) 101, 678, 713
MwoI GCNNNNNNNGC 5 cut(s) 45, 130, 182, 666, 675
NaeI GCCGGC 1 cut(s) 667
NarI GGCGCC 2 cut(s) 302, 663
NciI CCSGG 4 cut(s) 192, 281, 282, 730
NdeII GATC 5 cut(s) 144, 445, 450, 511, 523
NgoMIV GCCGGC 1 cut(s) 665
NlaIII CATG 2 cut(s) 433, 518
NlaIV GGNNCC 4 cut(s) 303, 664, 673, 674
NmeAIII GCCGAG 1 cut(s) 330
NmuCI GTSAC 1 cut(s) 163
NruI TCGCGA 1 cut(s) 101
NspV TTCGAA 1 cut(s) 70
PcsI WCGNNNNNNNCGW 4 cut(s) 221, 254, 257, 269
PdiI GCCGGC 1 cut(s) 667
PfeI GAWTC 2 cut(s) 361, 474
PflFI GACNNNGTC 2 cut(s) 220, 259
PkrI GCNGC 5 cut(s) 152, 178, 301, 491, 662
PleI GAGTC 1 cut(s) 263
PluTI GGCGCC 2 cut(s) 305, 666
PpsI GAGTC 1 cut(s) 263
Psp124BI GAGCTC 1 cut(s) 135
Psp6I CCWGG 1 cut(s) 309
PspGI CCWGG 1 cut(s) 309
PspN4I GGNNCC 4 cut(s) 303, 664, 673, 674
PspOMI GGGCCC 1 cut(s) 671
PspPI GGNCC 4 cut(s) 482, 671, 672, 726
PsuI RGATCY 1 cut(s) 144
PsyI GACNNNGTC 2 cut(s) 220, 259
RruI TCGCGA 1 cut(s) 101
RsaI GTAC 1 cut(s) 210
RsaNI GTAC 1 cut(s) 209
RseI CAYNNNNRTG 1 cut(s) 434
SacI GAGCTC 1 cut(s) 135
SacII CCGCGG 1 cut(s) 679
SalI GTCGAC 2 cut(s) 247, 253
SaqAI TTAA 1 cut(s) 54
SatI GCNGC 5 cut(s) 151, 177, 300, 490, 661
Sau3AI GATC 5 cut(s) 144, 445, 450, 511, 523
Sau96I GGNCC 4 cut(s) 482, 671, 672, 726
SchI GAGTC 1 cut(s) 263
ScrFI CCNGG 5 cut(s) 192, 281, 282, 311, 730
SduI GDGCHC 3 cut(s) 135, 429, 675
SfaNI GCATC 1 cut(s) 468
SfoI GGCGCC 2 cut(s) 303, 664
Sfr303I CCGCGG 1 cut(s) 679
SfuI TTCGAA 1 cut(s) 70
SgrAI CRCCGGYG 1 cut(s) 665
SgrBI CCGCGG 1 cut(s) 679
SgrDI CGTCGACG 2 cut(s) 247, 253
SinI GGWCC 1 cut(s) 726
SmaI CCCGGG 1 cut(s) 282
SmiMI CAYNNNNRTG 1 cut(s) 434
SmlI CTYRAG 1 cut(s) 34
SmoI CTYRAG 1 cut(s) 34
Sse9I AATT 3 cut(s) 28, 641, 704
SspDI GGCGCC 2 cut(s) 301, 662
SspI AATATT 1 cut(s) 14
SspMI CTAG 2 cut(s) 486, 745
SstI GAGCTC 1 cut(s) 135
StyD4I CCNGG 5 cut(s) 190, 279, 280, 309, 728
TaaI ACNGT 2 cut(s) 79, 222
TaiI ACGT 3 cut(s) 170, 254, 260
TaqI TCGA 6 cut(s) 70, 215, 224, 248, 254, 272
TasI AATT 3 cut(s) 28, 641, 704
TauI GCSGC 5 cut(s) 153, 179, 302, 492, 663
TfiI GAWTC 2 cut(s) 361, 474
Tru1I TTAA 1 cut(s) 54
Tru9I TTAA 1 cut(s) 54
TscAI CASTG 1 cut(s) 401
TseFI GTSAC 1 cut(s) 163
Tsp45I GTSAC 1 cut(s) 163
TspDTI ATGAA 5 cut(s) 446, 452, 608, 626, 704
TspMI CCCGGG 1 cut(s) 280
TspRI CASTG 1 cut(s) 401
Tth111I GACNNNGTC 2 cut(s) 220, 259
VpaK11BI GGWCC 1 cut(s) 726
XapI RAATTY 1 cut(s) 704
XmaI CCCGGG 1 cut(s) 280
XmiI GTMKAC 2 cut(s) 248, 254
XspI CTAG 2 cut(s) 486, 745
ZraI GACGTC 3 cut(s) 168, 252, 258
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.