pycom15g28020

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0010520
Physical Location & Seq
Forward (+)
23696355 .. 23697401
1047 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g28020.2

Sequence Viewer

Length: 654 bp
ATGTCGACATTCACATCAACTGATCTTTGCAAGCGGCATAGTCGCCTTTTCGTAAAGCCTCCTGGATTGTTCCGGCATGTTTCATCATCGACGTTGAAATGCCAGCAAGGTTTCAGAACCAACCATGCATTGTTGCCTCTCACAATTGGCCAAAGGAGTAGTCTCAGCAATCAACCATGTAGGAAGAACAAGATTGTATGCAATAGTTTTCCATTACCAGGAGTTCCAGATCCTTCCCACTCTTGGAAAGGATGGTTGATAGGGATTGTACTATCAGTAATATTACCCTTCTCGAGGAGCAAATGGGGGCCCTTGCTAGCATTGAAAAAGGAGGTGGACATGATTGTAGACACAGTTGAAGCAGTGGTGGAGGTGATAGAACAGGTAGCCGAGAAAGTGGAGGAAGTGGCAGATGATATAGGTGATAGTCTTCCTGAGGGAAAGCTTAAGACTGCACTTGAATTAGTTGAAAATGTAGCCAAAGAAGCAGCCAAAGATGCTCACCTTGCTGACGAGCTGATTGAGAAGGCGGAAGCACTGGAAGACCGGGTGGAAGACTTTTTTGAGTCAGCCTTGGATAAAGCAGGAGATGTTATCAAAGATGTTGGTGATGAATCAGATGTACAAATAGCTACAGAAAAGAAAACCCAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

218

Amino Acids

23.9

Weight (kDa)

5.22

Isoelectric Point (pI)

36.21

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 5, 348
AciI CCGC 2 cut(s) 34, 530
AclWI GGATC 1 cut(s) 224
AcoI YGGCCR 1 cut(s) 148
AfaI GTAC 2 cut(s) 270, 624
AfiI CCNNNNNNNGG 3 cut(s) 218, 243, 294
AflII CTTAAG 1 cut(s) 446
AgsI TTSAA 5 cut(s) 97, 325, 359, 461, 470
AjnI CCWGG 2 cut(s) 61, 217
AluBI AGCT 3 cut(s) 445, 517, 632
AluI AGCT 3 cut(s) 445, 517, 632
Alw26I GTCTC 1 cut(s) 167
AlwI GGATC 1 cut(s) 224
Ama87I CYCGRG 1 cut(s) 292
AoxI GGCC 2 cut(s) 148, 308
ApaI GGGCCC 1 cut(s) 312
ApeKI GCWGC 1 cut(s) 488
ArsI GACNNNNNNTTYG 2 cut(s) 145, 177
AspS9I GGNCC 2 cut(s) 308, 309
AsuC2I CCSGG 1 cut(s) 548
AsuHPI GGTGA 4 cut(s) 385, 434, 494, 620
AsuNHI GCTAGC 1 cut(s) 316
AvaI CYCGRG 1 cut(s) 292
AxyI CCTNAGG 1 cut(s) 435
BaeGI GKGCMC 1 cut(s) 312
BalI TGGCCA 1 cut(s) 150
BanII GRGCYC 1 cut(s) 312
BbsI GAAGAC 3 cut(s) 422, 549, 561
BbvI GCAGC 1 cut(s) 500
BccI CCATC 1 cut(s) 246
BciT130I CCWGG 2 cut(s) 63, 219
BcnI CCSGG 1 cut(s) 548
BcoDI GTCTC 1 cut(s) 167
BfaI CTAG 1 cut(s) 317
BfmI CTRYAG 1 cut(s) 633
BfrI CTTAAG 1 cut(s) 446
BisI GCNGC 2 cut(s) 35, 489
BlsI GCNGC 2 cut(s) 36, 490
Bme1390I CCNGG 3 cut(s) 63, 219, 548
BmeT110I CYCGRG 1 cut(s) 292
BmgT120I GGNCC 2 cut(s) 308, 309
BmiI GGNNCC 2 cut(s) 309, 310
BmrFI CCNGG 3 cut(s) 63, 219, 548
BmrI ACTGGG 1 cut(s) 643
BmsI GCATC 1 cut(s) 487
BmtI GCTAGC 1 cut(s) 320
BmuI ACTGGG 1 cut(s) 643
BpiI GAAGAC 3 cut(s) 422, 549, 561
BpuMI CCSGG 1 cut(s) 548
BsaJI CCNNGG 1 cut(s) 573
Bsc4I CCNNNNNNNGG 3 cut(s) 218, 243, 294
Bse1I ACTGG 2 cut(s) 543, 649
Bse21I CCTNAGG 1 cut(s) 435
BseBI CCWGG 2 cut(s) 63, 219
BseDI CCNNGG 1 cut(s) 573
BseGI GGATG 1 cut(s) 257
BseLI CCNNNNNNNGG 3 cut(s) 218, 243, 294
BseMII CTCAG 2 cut(s) 178, 426
BseNI ACTGG 2 cut(s) 543, 649
BseRI GAGGAG 1 cut(s) 310
BseSI GKGCMC 1 cut(s) 312
BseXI GCAGC 1 cut(s) 500
BsgI GTGCAG 1 cut(s) 438
BshFI GGCC 2 cut(s) 150, 310
BsiHKCI CYCGRG 1 cut(s) 292
BsiSI CCGG 2 cut(s) 73, 547
BslI CCNNNNNNNGG 3 cut(s) 218, 243, 294
BsmAI GTCTC 1 cut(s) 167
BsnI GGCC 2 cut(s) 150, 310
BsoBI CYCGRG 1 cut(s) 292
Bsp120I GGGCCC 1 cut(s) 308
Bsp1286I GDGCHC 1 cut(s) 312
Bsp1407I TGTACA 1 cut(s) 622
Bsp143I GATC 2 cut(s) 22, 229
BspACI CCGC 2 cut(s) 34, 530
BspANI GGCC 2 cut(s) 150, 310
BspCNI CTCAG 2 cut(s) 177, 427
BspLI GGNNCC 2 cut(s) 309, 310
BspOI GCTAGC 1 cut(s) 320
BspPI GGATC 1 cut(s) 224
BspTI CTTAAG 1 cut(s) 446
BsrGI TGTACA 1 cut(s) 622
BsrI ACTGG 2 cut(s) 543, 649
BssECI CCNNGG 1 cut(s) 573
BssMI GATC 2 cut(s) 22, 229
BssT1I CCWWGG 1 cut(s) 573
Bst2UI CCWGG 2 cut(s) 63, 219
Bst4CI ACNGT 1 cut(s) 355
BstAFI CTTAAG 1 cut(s) 446
BstAUI TGTACA 1 cut(s) 622
BstC8I GCNNGC 3 cut(s) 32, 104, 318
BstDEI CTNAG 2 cut(s) 164, 435
BstENI CCTNNNNNAGG 1 cut(s) 292
BstF5I GGATG 1 cut(s) 257
BstKTI GATC 2 cut(s) 25, 232
BstMAI GTCTC 1 cut(s) 167
BstMBI GATC 2 cut(s) 22, 229
BstMWI GCNNNNNNNGC 3 cut(s) 485, 497, 506
BstNI CCWGG 2 cut(s) 63, 219
BstNSI RCATGY 1 cut(s) 80
BstSCI CCNGG 3 cut(s) 61, 217, 546
BstSFI CTRYAG 1 cut(s) 633
BstSLI GKGCMC 1 cut(s) 312
BstV1I GCAGC 1 cut(s) 500
BstV2I GAAGAC 3 cut(s) 422, 549, 561
BstX2I RGATCY 1 cut(s) 229
BstYI RGATCY 1 cut(s) 229
Bsu36I CCTNAGG 1 cut(s) 435
BsuRI GGCC 2 cut(s) 150, 310
BtsCI GGATG 1 cut(s) 257
BtsI GCAGTG 1 cut(s) 369
BtsIMutI CAGTG 2 cut(s) 369, 536
Cac8I GCNNGC 3 cut(s) 32, 104, 318
Cfr13I GGNCC 2 cut(s) 308, 309
Csp6I GTAC 2 cut(s) 269, 623
CviAII CATG 4 cut(s) 77, 125, 177, 340
CviQI GTAC 2 cut(s) 269, 623
DdeI CTNAG 2 cut(s) 164, 435
DpnI GATC 2 cut(s) 24, 231
DpnII GATC 2 cut(s) 22, 229
EaeI YGGCCR 1 cut(s) 148
EciI GGCGGA 1 cut(s) 545
Eco130I CCWWGG 1 cut(s) 573
Eco24I GRGCYC 1 cut(s) 312
Eco81I CCTNAGG 1 cut(s) 435
Eco88I CYCGRG 1 cut(s) 292
EcoNI CCTNNNNNAGG 1 cut(s) 292
EcoO109I RGGNCCY 2 cut(s) 308, 309
EcoRII CCWGG 2 cut(s) 61, 217
EcoT14I CCWWGG 1 cut(s) 573
EcoT22I ATGCAT 1 cut(s) 130
EcoT38I GRGCYC 1 cut(s) 312
ErhI CCWWGG 1 cut(s) 573
FaeI CATG 4 cut(s) 80, 128, 180, 343
FaiI YATR 7 cut(s) 39, 78, 126, 178, 199, 341, 419
FatI CATG 4 cut(s) 76, 124, 176, 339
FblI GTMKAC 2 cut(s) 5, 348
Fnu4HI GCNGC 2 cut(s) 35, 489
FokI GGATG 1 cut(s) 264
FriOI GRGCYC 1 cut(s) 312
Fsp4HI GCNGC 2 cut(s) 35, 489
FspBI CTAG 1 cut(s) 317
GluI GCNGC 2 cut(s) 35, 489
HaeIII GGCC 2 cut(s) 150, 310
HapII CCGG 2 cut(s) 73, 547
Hin1II CATG 4 cut(s) 80, 128, 180, 343
HincII GTYRAC 1 cut(s) 6
HindII GTYRAC 1 cut(s) 6
HindIII AAGCTT 1 cut(s) 443
HinfI GANTC 2 cut(s) 566, 614
HpaII CCGG 2 cut(s) 73, 547
HphI GGTGA 4 cut(s) 385, 434, 494, 620
Hpy166II GTNNAC 3 cut(s) 6, 337, 349
Hpy188I TCNGA 2 cut(s) 116, 619
Hpy188III TCNNGA 3 cut(s) 227, 292, 434
Hpy8I GTNNAC 3 cut(s) 6, 337, 349
Hpy99I CGWCG 1 cut(s) 94
HpyAV CCTTC 3 cut(s) 243, 298, 520
HpyCH4III ACNGT 1 cut(s) 355
HpyCH4IV ACGT 1 cut(s) 92
HpyCH4V TGCA 4 cut(s) 30, 128, 201, 455
HpyF10VI GCNNNNNNNGC 3 cut(s) 485, 497, 506
HpyF3I CTNAG 2 cut(s) 164, 435
HpySE526I ACGT 1 cut(s) 92
Hsp92II CATG 4 cut(s) 80, 128, 180, 343
Kzo9I GATC 2 cut(s) 22, 229
LmnI GCTCC 1 cut(s) 297
Lsp1109I GCAGC 1 cut(s) 500
LweI GCATC 1 cut(s) 487
MaeI CTAG 1 cut(s) 317
MaeII ACGT 1 cut(s) 92
MalI GATC 2 cut(s) 24, 231
MboI GATC 2 cut(s) 22, 229
MboII GAAGA 4 cut(s) 196, 422, 554, 566
MfeI CAATTG 1 cut(s) 144
MflI RGATCY 1 cut(s) 229
MhlI GDGCHC 1 cut(s) 312
MlsI TGGCCA 1 cut(s) 150
MluCI AATT 2 cut(s) 144, 461
MluNI TGGCCA 1 cut(s) 150
MlyI GAGTC 1 cut(s) 575
MnlI CCTC 7 cut(s) 69, 147, 288, 325, 364, 394, 430
Mox20I TGGCCA 1 cut(s) 150
Mph1103I ATGCAT 1 cut(s) 130
MscI TGGCCA 1 cut(s) 150
MseI TTAA 1 cut(s) 447
Msp20I TGGCCA 1 cut(s) 150
MspCI CTTAAG 1 cut(s) 446
MspI CCGG 2 cut(s) 73, 547
MspR9I CCNGG 3 cut(s) 63, 219, 548
MunI CAATTG 1 cut(s) 144
MvaI CCWGG 2 cut(s) 63, 219
MwoI GCNNNNNNNGC 3 cut(s) 485, 497, 506
NciI CCSGG 1 cut(s) 548
NdeII GATC 2 cut(s) 22, 229
NheI GCTAGC 1 cut(s) 316
NlaIII CATG 4 cut(s) 80, 128, 180, 343
NlaIV GGNNCC 2 cut(s) 309, 310
NmeAIII GCCGAG 1 cut(s) 415
NsiI ATGCAT 1 cut(s) 130
NspI RCATGY 1 cut(s) 80
PaeR7I CTCGAG 1 cut(s) 292
PfeI GAWTC 1 cut(s) 614
PfoI TCCNGGA 1 cut(s) 61
PkrI GCNGC 2 cut(s) 36, 490
PleI GAGTC 1 cut(s) 574
PpsI GAGTC 1 cut(s) 574
Psp6I CCWGG 2 cut(s) 61, 217
PspGI CCWGG 2 cut(s) 61, 217
PspN4I GGNNCC 2 cut(s) 309, 310
PspOMI GGGCCC 1 cut(s) 308
PspPI GGNCC 2 cut(s) 308, 309
PsuI RGATCY 1 cut(s) 229
RsaI GTAC 2 cut(s) 270, 624
RsaNI GTAC 2 cut(s) 269, 623
SalI GTCGAC 1 cut(s) 4
SaqAI TTAA 1 cut(s) 447
SatI GCNGC 2 cut(s) 35, 489
Sau3AI GATC 2 cut(s) 22, 229
Sau96I GGNCC 2 cut(s) 308, 309
SchI GAGTC 1 cut(s) 575
ScrFI CCNGG 3 cut(s) 63, 219, 548
SduI GDGCHC 1 cut(s) 312
SfaNI GCATC 1 cut(s) 487
SfcI CTRYAG 1 cut(s) 633
Sfr274I CTCGAG 1 cut(s) 292
SlaI CTCGAG 1 cut(s) 292
SmlI CTYRAG 2 cut(s) 292, 446
SmoI CTYRAG 2 cut(s) 292, 446
Sse9I AATT 2 cut(s) 144, 461
SsiI CCGC 2 cut(s) 34, 530
SspI AATATT 1 cut(s) 282
SspMI CTAG 1 cut(s) 317
StyD4I CCNGG 3 cut(s) 61, 217, 546
StyI CCWWGG 1 cut(s) 573
TaaI ACNGT 1 cut(s) 355
TaiI ACGT 1 cut(s) 95
TaqI TCGA 3 cut(s) 5, 89, 293
TasI AATT 2 cut(s) 144, 461
TatI WGTACW 2 cut(s) 268, 622
TauI GCSGC 1 cut(s) 37
TfiI GAWTC 1 cut(s) 614
Tru1I TTAA 1 cut(s) 447
Tru9I TTAA 1 cut(s) 447
TscAI CASTG 2 cut(s) 369, 543
TseI GCWGC 1 cut(s) 488
TspDTI ATGAA 2 cut(s) 72, 627
TspRI CASTG 2 cut(s) 369, 543
Vha464I CTTAAG 1 cut(s) 446
XagI CCTNNNNNAGG 1 cut(s) 292
XceI RCATGY 1 cut(s) 80
XhoI CTCGAG 1 cut(s) 292
XmiI GTMKAC 2 cut(s) 5, 348
XspI CTAG 1 cut(s) 317
Zsp2I ATGCAT 1 cut(s) 130
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.