pycom15g28030

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0010520
Physical Location & Seq
Forward (+)
23701412 .. 23703466
2055 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g28030.2

Sequence Viewer

Length: 660 bp
ATGTCAATAATCACAACATCAATTGATCTAAGTCGATCGGCCGGCGGTTGCGTTTTTGTGAAGCGTCCTGGCGGAGTAGTAGTTCAACCATTGTCATCATCATCAACATGGCAATGCCAACAAGGTATCAGAAGCAACCCTGCATTATTGTCTCTAATTAGCAGCCAACCATGTCGGAATAAGATTATATGCAGTAGCTCTACATCACCTGGAGATCAAAGTCCTTCTGTAGAGCATTCAAACTCTTGGAGAGGCTGGATGATAGGGATGGTATTCTCAGTAATTATACCTTTTTGGAGGCACAAATGGGGGCCTTTGCGACAATTGAAGAAGGAGGTGGACATGGTTGTAAACAATGTTGAAGCAGTGGTGGAGGTGGTAGAAAAGGTGGCAGAAAAAGTGGAGGAAGTGGCAGACGAAATAGGTGATCACCTTCCAGCTGATGGAAAGTTTAGGGCTGCTTCTGAAGTAGTTGAAGGAATAGCCAGAGAAGTAGCCAAAGATGCACACCTTGCAGACCAGCTAATTGAGAAGGCGGAAGCACTAGAAGATCAGGTGGAAGACTTTTTTGAGTCAGCCATGGATAATGTAGGAAATGCGACCAAAGATGTTAGCGATGAATCAAATGTACAAGTAGCTACGGAAAAGAAAATCCAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

220

Amino Acids

23.91

Weight (kDa)

4.93

Isoelectric Point (pI)

45.41

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 443
AciI CCGC 3 cut(s) 45, 72, 536
AcoI YGGCCR 1 cut(s) 39
AcuI CTGAAG 1 cut(s) 486
AfaI GTAC 1 cut(s) 630
AfiI CCNNNNNNNGG 1 cut(s) 443
AgsI TTSAA 5 cut(s) 86, 240, 328, 362, 476
AjnI CCWGG 2 cut(s) 67, 208
AloI GAACNNNNNNTCC 2 cut(s) 66, 98
AluBI AGCT 4 cut(s) 198, 440, 523, 638
AluI AGCT 4 cut(s) 198, 440, 523, 638
Alw26I GTCTC 1 cut(s) 156
AoxI GGCC 2 cut(s) 39, 311
ApeKI GCWGC 2 cut(s) 162, 458
AspS9I GGNCC 1 cut(s) 311
AsuHPI GGTGA 3 cut(s) 198, 422, 437
BbsI GAAGAC 1 cut(s) 567
BbvI GCAGC 2 cut(s) 174, 445
BccI CCATC 2 cut(s) 262, 437
BciT130I CCWGG 2 cut(s) 69, 210
BclI TGATCA 1 cut(s) 427
BcoDI GTCTC 1 cut(s) 156
BfaI CTAG 1 cut(s) 545
BfmI CTRYAG 1 cut(s) 228
BisI GCNGC 2 cut(s) 163, 459
BlsI GCNGC 2 cut(s) 164, 460
Bme1390I CCNGG 2 cut(s) 69, 210
BmgT120I GGNCC 1 cut(s) 311
BmiI GGNNCC 1 cut(s) 312
BmrFI CCNGG 2 cut(s) 69, 210
BmsI GCATC 1 cut(s) 493
BpiI GAAGAC 1 cut(s) 567
BpmI CTGGAG 1 cut(s) 231
BsaJI CCNNGG 1 cut(s) 579
BsaXI ACNNNNNCTCC 2 cut(s) 66, 96
Bsc4I CCNNNNNNNGG 1 cut(s) 443
Bse118I RCCGGY 1 cut(s) 41
Bse1I ACTGG 1 cut(s) 655
Bse3DI GCAATG 1 cut(s) 119
BseBI CCWGG 2 cut(s) 69, 210
BseDI CCNNGG 1 cut(s) 579
BseGI GGATG 2 cut(s) 264, 273
BseLI CCNNNNNNNGG 1 cut(s) 443
BseMI GCAATG 1 cut(s) 119
BseMII CTCAG 1 cut(s) 291
BseNI ACTGG 1 cut(s) 655
BseX3I CGGCCG 1 cut(s) 39
BseXI GCAGC 2 cut(s) 174, 445
Bsh1285I CGRYCG 2 cut(s) 38, 42
BshFI GGCC 2 cut(s) 41, 313
BsiEI CGRYCG 2 cut(s) 38, 42
BsiSI CCGG 1 cut(s) 42
BslI CCNNNNNNNGG 1 cut(s) 443
BsmAI GTCTC 1 cut(s) 156
BsmI GAATGC 1 cut(s) 235
BsnI GGCC 2 cut(s) 41, 313
Bsp1407I TGTACA 1 cut(s) 628
Bsp143I GATC 5 cut(s) 25, 35, 214, 427, 550
Bsp19I CCATGG 1 cut(s) 579
BspACI CCGC 3 cut(s) 45, 72, 536
BspANI GGCC 2 cut(s) 41, 313
BspCNI CTCAG 1 cut(s) 290
BspLI GGNNCC 1 cut(s) 312
BsrDI GCAATG 1 cut(s) 119
BsrFI RCCGGY 1 cut(s) 41
BsrGI TGTACA 1 cut(s) 628
BsrI ACTGG 1 cut(s) 655
BssAI RCCGGY 1 cut(s) 41
BssECI CCNNGG 1 cut(s) 579
BssMI GATC 5 cut(s) 25, 35, 214, 427, 550
BssT1I CCWWGG 1 cut(s) 579
Bst2UI CCWGG 2 cut(s) 69, 210
BstAPI GCANNNNNTGC 1 cut(s) 512
BstAUI TGTACA 1 cut(s) 628
BstC8I GCNNGC 1 cut(s) 43
BstDEI CTNAG 2 cut(s) 29, 277
BstDSI CCRYGG 1 cut(s) 579
BstF5I GGATG 2 cut(s) 264, 273
BstKTI GATC 5 cut(s) 28, 38, 217, 430, 553
BstMAI GTCTC 1 cut(s) 156
BstMBI GATC 5 cut(s) 25, 35, 214, 427, 550
BstMCI CGRYCG 2 cut(s) 38, 42
BstMWI GCNNNNNNNGC 2 cut(s) 503, 512
BstNI CCWGG 2 cut(s) 69, 210
BstSCI CCNGG 2 cut(s) 67, 208
BstSFI CTRYAG 1 cut(s) 228
BstV1I GCAGC 2 cut(s) 174, 445
BstV2I GAAGAC 1 cut(s) 567
BstZI CGGCCG 1 cut(s) 39
BsuRI GGCC 2 cut(s) 41, 313
BtgI CCRYGG 1 cut(s) 579
BtgZI GCGATG 1 cut(s) 630
BtsCI GGATG 2 cut(s) 264, 273
BtsI GCAGTG 1 cut(s) 372
BtsIMutI CAGTG 1 cut(s) 372
Cac8I GCNNGC 1 cut(s) 43
Cfr10I RCCGGY 1 cut(s) 41
Cfr13I GGNCC 1 cut(s) 311
CseI GACGC 1 cut(s) 53
Csp6I GTAC 1 cut(s) 629
CviAII CATG 4 cut(s) 108, 171, 343, 580
CviQI GTAC 1 cut(s) 629
DdeI CTNAG 2 cut(s) 29, 277
DpnI GATC 5 cut(s) 27, 37, 216, 429, 552
DpnII GATC 5 cut(s) 25, 35, 214, 427, 550
EaeI YGGCCR 1 cut(s) 39
EagI CGGCCG 1 cut(s) 39
EciI GGCGGA 2 cut(s) 87, 551
EclXI CGGCCG 1 cut(s) 39
Eco130I CCWWGG 1 cut(s) 579
Eco52I CGGCCG 1 cut(s) 39
Eco57I CTGAAG 1 cut(s) 486
EcoO109I RGGNCCY 1 cut(s) 311
EcoRII CCWGG 2 cut(s) 67, 208
EcoT14I CCWWGG 1 cut(s) 579
ErhI CCWWGG 1 cut(s) 579
FaeI CATG 4 cut(s) 111, 174, 346, 583
FaiI YATR 7 cut(s) 109, 172, 188, 190, 287, 344, 581
FatI CATG 4 cut(s) 107, 170, 342, 579
FbaI TGATCA 1 cut(s) 427
Fnu4HI GCNGC 2 cut(s) 163, 459
FokI GGATG 2 cut(s) 271, 280
Fsp4HI GCNGC 2 cut(s) 163, 459
FspBI CTAG 1 cut(s) 545
GluI GCNGC 2 cut(s) 163, 459
GsuI CTGGAG 1 cut(s) 231
HaeIII GGCC 2 cut(s) 41, 313
HapII CCGG 1 cut(s) 42
HgaI GACGC 1 cut(s) 53
Hin1II CATG 4 cut(s) 111, 174, 346, 583
HinfI GANTC 2 cut(s) 572, 620
HpaII CCGG 1 cut(s) 42
HphI GGTGA 3 cut(s) 198, 422, 437
Hpy166II GTNNAC 2 cut(s) 340, 352
Hpy188I TCNGA 3 cut(s) 131, 177, 466
Hpy8I GTNNAC 2 cut(s) 340, 352
HpyAV CCTTC 5 cut(s) 234, 325, 443, 470, 526
HpyCH4V TGCA 4 cut(s) 143, 192, 506, 515
HpyF10VI GCNNNNNNNGC 2 cut(s) 503, 512
HpyF3I CTNAG 2 cut(s) 29, 277
Hsp92II CATG 4 cut(s) 111, 174, 346, 583
KroI GCCGGC 1 cut(s) 41
KroNI GCCGGC 1 cut(s) 43
Ksp22I TGATCA 1 cut(s) 427
Kzo9I GATC 5 cut(s) 25, 35, 214, 427, 550
Lsp1109I GCAGC 2 cut(s) 174, 445
LweI GCATC 1 cut(s) 493
MaeI CTAG 1 cut(s) 545
MalI GATC 5 cut(s) 27, 37, 216, 429, 552
MboI GATC 5 cut(s) 25, 35, 214, 427, 550
MboII GAAGA 3 cut(s) 340, 560, 572
MfeI CAATTG 2 cut(s) 21, 323
MluCI AATT 5 cut(s) 21, 156, 282, 323, 525
MlyI GAGTC 1 cut(s) 581
MmeI TCCRAC 1 cut(s) 155
MnlI CCTC 5 cut(s) 245, 291, 328, 367, 397
MroNI GCCGGC 1 cut(s) 41
MslI CAYNNNNRTG 2 cut(s) 106, 112
MspA1I CMGCKG 1 cut(s) 440
MspI CCGG 1 cut(s) 42
MspR9I CCNGG 2 cut(s) 69, 210
MunI CAATTG 2 cut(s) 21, 323
Mva1269I GAATGC 1 cut(s) 235
MvaI CCWGG 2 cut(s) 69, 210
MwoI GCNNNNNNNGC 2 cut(s) 503, 512
NaeI GCCGGC 1 cut(s) 43
NcoI CCATGG 1 cut(s) 579
NdeII GATC 5 cut(s) 25, 35, 214, 427, 550
NgoMIV GCCGGC 1 cut(s) 41
NlaIII CATG 4 cut(s) 111, 174, 346, 583
NlaIV GGNNCC 1 cut(s) 312
PctI GAATGC 1 cut(s) 235
PdiI GCCGGC 1 cut(s) 43
PfeI GAWTC 1 cut(s) 620
PflMI CCANNNNNTGG 1 cut(s) 443
PkrI GCNGC 2 cut(s) 164, 460
Ple19I CGATCG 1 cut(s) 38
PleI GAGTC 1 cut(s) 580
PpsI GAGTC 1 cut(s) 580
Psp6I CCWGG 2 cut(s) 67, 208
PspGI CCWGG 2 cut(s) 67, 208
PspN4I GGNNCC 1 cut(s) 312
PspPI GGNCC 1 cut(s) 311
PvuI CGATCG 1 cut(s) 38
PvuII CAGCTG 1 cut(s) 440
RsaI GTAC 1 cut(s) 630
RsaNI GTAC 1 cut(s) 629
RseI CAYNNNNRTG 2 cut(s) 106, 112
SatI GCNGC 2 cut(s) 163, 459
Sau3AI GATC 5 cut(s) 25, 35, 214, 427, 550
Sau96I GGNCC 1 cut(s) 311
SchI GAGTC 1 cut(s) 581
ScrFI CCNGG 2 cut(s) 69, 210
SfaNI GCATC 1 cut(s) 493
SfcI CTRYAG 1 cut(s) 228
SmiMI CAYNNNNRTG 2 cut(s) 106, 112
Sse9I AATT 5 cut(s) 21, 156, 282, 323, 525
SsiI CCGC 3 cut(s) 45, 72, 536
SspMI CTAG 1 cut(s) 545
StyD4I CCNGG 2 cut(s) 67, 208
StyI CCWWGG 1 cut(s) 579
TaqI TCGA 1 cut(s) 34
TasI AATT 5 cut(s) 21, 156, 282, 323, 525
TatI WGTACW 1 cut(s) 628
TfiI GAWTC 1 cut(s) 620
TscAI CASTG 1 cut(s) 372
TseI GCWGC 2 cut(s) 162, 458
TspDTI ATGAA 1 cut(s) 633
TspGWI ACGGA 1 cut(s) 656
TspRI CASTG 1 cut(s) 372
Van91I CCANNNNNTGG 1 cut(s) 443
XspI CTAG 1 cut(s) 545
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.