pycom15g28860

Belongs to the bacterial ribosomal protein bL17 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0008477
Physical Location & Seq
Forward (+)
25242689 .. 25245861
3173 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 414 bp
ATGACGAAGTTCAGGAAGCTAAACCGACCCACGGGTCACCGCATGTCCATGCTCAGGACGATGGTGTCGCAGTTGGTGAAACATGAGCGCATTGAGACCACTGTTTCCAAGGCCAAAGAGATCCGACGCGAGGCTGATAAAATGGTGCAGCTTGGAAAAGATGGCTCCTTGTGTGCCGCAAGGCGTGCTGCTGCTTTTGTGCGTGGGGATGATGTACTTCACAAGCTATTTACAGAACTGGCTTATCGATACAAGGATCGAGTAGGCGGATACACAAGACTACTTAGGACTCGTATACGAGTTGGTGATGCTGCACCTATGGCCTATATTGAGTTCATTGACAGAGAAAATGAGCTCAGACAATCAAAACTACCAGCCCCTCAACCACCAGAGAGAGTTCCTTTAGATCCTTGA

Protein Analysis

138

Amino Acids

15.84

Weight (kDa)

10.34

Isoelectric Point (pI)

49.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L17 PF01196 17 - 113 5.7e-35 Ribosomal protein L17
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 33, 64
AccI GTMKAC 1 cut(s) 295
AccII CGCG 1 cut(s) 129
AciI CCGC 3 cut(s) 40, 177, 267
AclWI GGATC 3 cut(s) 115, 264, 401
AfaI GTAC 1 cut(s) 216
AfiI CCNNNNNNNGG 3 cut(s) 31, 54, 130
AluBI AGCT 4 cut(s) 19, 151, 226, 355
AluI AGCT 4 cut(s) 19, 151, 226, 355
Alw21I GWGCWC 1 cut(s) 357
Alw26I GTCTC 1 cut(s) 89
AlwI GGATC 3 cut(s) 115, 264, 401
AoxI GGCC 2 cut(s) 111, 321
ApeKI GCWGC 4 cut(s) 148, 188, 191, 311
AspLEI GCGC 1 cut(s) 90
AsuHPI GGTGA 3 cut(s) 29, 88, 317
BanII GRGCYC 1 cut(s) 357
Bbv12I GWGCWC 1 cut(s) 357
BbvI GCAGC 4 cut(s) 160, 175, 178, 298
BccI CCATC 2 cut(s) 55, 155
BciVI GTATCC 1 cut(s) 263
BcoDI GTCTC 1 cut(s) 89
BfuI GTATCC 1 cut(s) 263
BisI GCNGC 5 cut(s) 149, 177, 189, 192, 312
BlsI GCNGC 5 cut(s) 150, 178, 190, 193, 313
BmiI GGNNCC 1 cut(s) 166
BmsI GCATC 1 cut(s) 298
Bpu10I CCTNAGC 1 cut(s) 53
Bsa29I ATCGAT 1 cut(s) 247
BsaI GGTCTC 1 cut(s) 89
BsaJI CCNNGG 2 cut(s) 30, 108
Bsc4I CCNNNNNNNGG 3 cut(s) 31, 54, 130
Bse1I ACTGG 1 cut(s) 243
BseCI ATCGAT 1 cut(s) 247
BseDI CCNNGG 2 cut(s) 30, 108
BseGI GGATG 1 cut(s) 214
BseLI CCNNNNNNNGG 3 cut(s) 31, 54, 130
BseMII CTCAG 2 cut(s) 67, 370
BseNI ACTGG 1 cut(s) 243
BseXI GCAGC 4 cut(s) 160, 175, 178, 298
BsgI GTGCAG 2 cut(s) 167, 297
Bsh1236I CGCG 1 cut(s) 129
BshFI GGCC 2 cut(s) 113, 323
BshVI ATCGAT 1 cut(s) 247
BsiHKAI GWGCWC 1 cut(s) 357
BslI CCNNNNNNNGG 3 cut(s) 31, 54, 130
BsmAI GTCTC 1 cut(s) 89
BsnI GGCC 2 cut(s) 113, 323
Bso31I GGTCTC 1 cut(s) 89
Bsp1286I GDGCHC 1 cut(s) 357
Bsp143I GATC 3 cut(s) 120, 256, 406
BspACI CCGC 3 cut(s) 40, 177, 267
BspANI GGCC 2 cut(s) 113, 323
BspCNI CTCAG 2 cut(s) 66, 369
BspDI ATCGAT 1 cut(s) 247
BspFNI CGCG 1 cut(s) 129
BspLI GGNNCC 1 cut(s) 166
BspPI GGATC 3 cut(s) 115, 264, 401
BspTNI GGTCTC 1 cut(s) 89
BsrI ACTGG 1 cut(s) 243
BssECI CCNNGG 2 cut(s) 30, 108
BssMI GATC 3 cut(s) 120, 256, 406
BssNAI GTATAC 1 cut(s) 296
BssT1I CCWWGG 1 cut(s) 108
Bst1107I GTATAC 1 cut(s) 296
Bst4CI ACNGT 1 cut(s) 103
BstAPI GCANNNNNTGC 1 cut(s) 185
BstC8I GCNNGC 1 cut(s) 186
BstDEI CTNAG 3 cut(s) 53, 284, 356
BstDSI CCRYGG 1 cut(s) 30
BstEII GGTNACC 1 cut(s) 35
BstF5I GGATG 1 cut(s) 214
BstFNI CGCG 1 cut(s) 129
BstHHI GCGC 1 cut(s) 90
BstKTI GATC 3 cut(s) 123, 259, 409
BstMAI GTCTC 1 cut(s) 89
BstMBI GATC 3 cut(s) 120, 256, 406
BstMWI GCNNNNNNNGC 2 cut(s) 185, 320
BstNSI RCATGY 1 cut(s) 46
BstPI GGTNACC 1 cut(s) 35
BstUI CGCG 1 cut(s) 129
BstV1I GCAGC 4 cut(s) 160, 175, 178, 298
BstX2I RGATCY 2 cut(s) 120, 406
BstYI RGATCY 2 cut(s) 120, 406
BstZ17I GTATAC 1 cut(s) 296
Bsu15I ATCGAT 1 cut(s) 247
BsuI GTATCC 1 cut(s) 263
BsuRI GGCC 2 cut(s) 113, 323
BsuTUI ATCGAT 1 cut(s) 247
BtgI CCRYGG 1 cut(s) 30
BtsCI GGATG 1 cut(s) 214
BtsIMutI CAGTG 1 cut(s) 99
Cac8I GCNNGC 1 cut(s) 186
CfoI GCGC 1 cut(s) 90
ClaI ATCGAT 1 cut(s) 247
CseI GACGC 1 cut(s) 135
Csp6I GTAC 1 cut(s) 215
CviAII CATG 3 cut(s) 43, 49, 83
CviQI GTAC 1 cut(s) 215
DdeI CTNAG 3 cut(s) 53, 284, 356
DpnI GATC 3 cut(s) 122, 258, 408
DpnII GATC 3 cut(s) 120, 256, 406
DrdI GACNNNNNNGTC 2 cut(s) 33, 64
DseDI GACNNNNNNGTC 2 cut(s) 33, 64
EciI GGCGGA 1 cut(s) 282
Ecl136II GAGCTC 1 cut(s) 355
Eco130I CCWWGG 1 cut(s) 108
Eco24I GRGCYC 1 cut(s) 357
Eco31I GGTCTC 1 cut(s) 89
Eco53kI GAGCTC 1 cut(s) 355
Eco91I GGTNACC 1 cut(s) 35
EcoICRI GAGCTC 1 cut(s) 355
EcoO65I GGTNACC 1 cut(s) 35
EcoT14I CCWWGG 1 cut(s) 108
EcoT38I GRGCYC 1 cut(s) 357
ErhI CCWWGG 1 cut(s) 108
FaeI CATG 3 cut(s) 46, 52, 86
FaiI YATR 6 cut(s) 44, 50, 84, 296, 320, 327
FatI CATG 3 cut(s) 42, 48, 82
FblI GTMKAC 1 cut(s) 295
Fnu4HI GCNGC 5 cut(s) 149, 177, 189, 192, 312
FokI GGATG 1 cut(s) 221
FriOI GRGCYC 1 cut(s) 357
Fsp4HI GCNGC 5 cut(s) 149, 177, 189, 192, 312
GlaI GCGC 1 cut(s) 89
GluI GCNGC 5 cut(s) 149, 177, 189, 192, 312
HaeIII GGCC 2 cut(s) 113, 323
HgaI GACGC 1 cut(s) 135
HhaI GCGC 1 cut(s) 90
Hin1II CATG 3 cut(s) 46, 52, 86
Hin6I GCGC 1 cut(s) 88
HinP1I GCGC 1 cut(s) 88
HinfI GANTC 1 cut(s) 289
HphI GGTGA 3 cut(s) 29, 88, 317
Hpy166II GTNNAC 1 cut(s) 296
Hpy188I TCNGA 2 cut(s) 125, 359
Hpy188III TCNNGA 2 cut(s) 13, 55
Hpy8I GTNNAC 1 cut(s) 296
Hpy99I CGWCG 1 cut(s) 129
HpyCH4III ACNGT 1 cut(s) 103
HpyCH4V TGCA 2 cut(s) 148, 314
HpyF10VI GCNNNNNNNGC 2 cut(s) 185, 320
HpyF3I CTNAG 3 cut(s) 53, 284, 356
Hsp92II CATG 3 cut(s) 46, 52, 86
HspAI GCGC 1 cut(s) 88
Kzo9I GATC 3 cut(s) 120, 256, 406
LmnI GCTCC 1 cut(s) 170
LpnPI CCDG 4 cut(s) 40, 224, 387, 402
Lsp1109I GCAGC 4 cut(s) 160, 175, 178, 298
LweI GCATC 1 cut(s) 298
MaeIII GTNAC 1 cut(s) 35
MalI GATC 3 cut(s) 122, 258, 408
MboI GATC 3 cut(s) 120, 256, 406
MflI RGATCY 2 cut(s) 120, 406
MhlI GDGCHC 1 cut(s) 357
MlyI GAGTC 1 cut(s) 283
MmeI TCCRAC 1 cut(s) 148
MnlI CCTC 2 cut(s) 124, 390
MslI CAYNNNNRTG 1 cut(s) 47
MvnI CGCG 1 cut(s) 129
MwoI GCNNNNNNNGC 2 cut(s) 185, 320
NdeII GATC 3 cut(s) 120, 256, 406
NlaIII CATG 3 cut(s) 46, 52, 86
NlaIV GGNNCC 1 cut(s) 166
NmuCI GTSAC 1 cut(s) 35
NspI RCATGY 1 cut(s) 46
PkrI GCNGC 5 cut(s) 150, 178, 190, 193, 313
PleI GAGTC 1 cut(s) 283
PpsI GAGTC 1 cut(s) 283
Psp124BI GAGCTC 1 cut(s) 357
PspEI GGTNACC 1 cut(s) 35
PspN4I GGNNCC 1 cut(s) 166
PsuI RGATCY 2 cut(s) 120, 406
RsaI GTAC 1 cut(s) 216
RsaNI GTAC 1 cut(s) 215
RseI CAYNNNNRTG 1 cut(s) 47
SacI GAGCTC 1 cut(s) 357
SatI GCNGC 5 cut(s) 149, 177, 189, 192, 312
Sau3AI GATC 3 cut(s) 120, 256, 406
SchI GAGTC 1 cut(s) 283
SduI GDGCHC 1 cut(s) 357
SetI ASST 5 cut(s) 21, 153, 228, 319, 357
SfaNI GCATC 1 cut(s) 298
SmiMI CAYNNNNRTG 1 cut(s) 47
SsiI CCGC 3 cut(s) 40, 177, 267
SstI GAGCTC 1 cut(s) 357
StyI CCWWGG 1 cut(s) 108
TaaI ACNGT 1 cut(s) 103
TaqI TCGA 2 cut(s) 247, 259
TatI WGTACW 1 cut(s) 214
TauI GCSGC 1 cut(s) 179
TscAI CASTG 1 cut(s) 106
TseFI GTSAC 1 cut(s) 35
TseI GCWGC 4 cut(s) 148, 188, 191, 311
Tsp45I GTSAC 1 cut(s) 35
TspDTI ATGAA 1 cut(s) 325
TspRI CASTG 1 cut(s) 106
XceI RCATGY 1 cut(s) 46
XmiI GTMKAC 1 cut(s) 295
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.