pycom15g34630

belongs to the flavoprotein pyridine nucleotide cytochrome reductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0005981
Physical Location & Seq
Reverse (-)
34415652 .. 34418046
2395 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 765 bp
ATGAGTTCGAATTCGGATGTGGTACGCGCGTTGGAGTCGGCGCTGGGGGTTTCGTTCGGTGGTTCGGTGACGGACACGGTGGTGGTGATCGCGACGACTTCAATTGCGCTGATAATTGGGGTTTTGGTATTGGTGTGGAGGAGATCGTCGGATCGGAGCCGGGAGGTGAAGCGGCTGGTGTTGCCCAAGCCGCTTGCTGTGGAGGAAGAGGAAGACGAGTTTGAGGTTGCGGCGGGGAAGACTCGGGTCACCATCTTCTATGGAACTCAGACTGGTACAGCTGAGGGATTCGCTAAGGCTTTGGCTGAGGAGATTAAGGCTAGATATGAGAAAGCTGCTGTGAAGGTTGTCGACCTGGATGACTATGCTGAGGATGATGATAAATATGAAGAAAAACTGAAGAAAGAGACTTTGGCATTTTTTATGTTGGCCACTTATGGAGATGGGGAGCCAACTGATAATGCAGTAAGATTTTACAAATGGTTTACGGAGGGAAAAGATAGGGGTGATTGGCTTCAACATCTAAGATATGGCGTTTTTGCTCTGGGTAACCGACAATATGAACATTTCAATAAGGTAGGAAAAGTGGTTGATGATAAACTTAGTGAAGAAGGTGCAAAGCGTCTTGTTCCTGTTGGCTTAGGTGATGATGACCAGTGCATCGAGGATGATTTTTCTGCTTGGAGGGAATCATTGTGGCCTGAGTTGGATCGATCGGAGATGAAGATGATACAAATTCTGCGGCTACTCCATATACAGCTGTAA

Protein Analysis

255

Amino Acids

28.68

Weight (kDa)

4.85

Isoelectric Point (pI)

42.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Flavodoxin_1 PF00258 85 - 228 3.6e-36 Flavodoxin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 351
AccII CGCG 3 cut(s) 27, 29, 92
AciI CCGC 5 cut(s) 172, 191, 230, 233, 742
AclWI GGATC 2 cut(s) 159, 717
AcoI YGGCCR 1 cut(s) 429
AcsI RAATTY 2 cut(s) 10, 735
AcuI CTGAAG 1 cut(s) 419
AfaI GTAC 2 cut(s) 24, 277
AgsI TTSAA 3 cut(s) 102, 518, 571
AjnI CCWGG 1 cut(s) 354
AjuI GAANNNNNNNTTGG 2 cut(s) 395, 427
AleI CACNNNNGTG 1 cut(s) 80
AluBI AGCT 3 cut(s) 281, 335, 760
AluI AGCT 3 cut(s) 281, 335, 760
Alw26I GTCTC 1 cut(s) 401
AlwI GGATC 2 cut(s) 159, 717
Ama87I CYCGRG 1 cut(s) 243
AoxI GGCC 2 cut(s) 429, 698
ApeKI GCWGC 1 cut(s) 335
ApoI RAATTY 2 cut(s) 10, 735
AspLEI GCGC 3 cut(s) 29, 43, 109
AsuC2I CCSGG 1 cut(s) 161
AsuHPI GGTGA 6 cut(s) 79, 97, 178, 241, 518, 656
AsuII TTCGAA 1 cut(s) 8
AvaI CYCGRG 1 cut(s) 243
BaeI ACNNNNGTAYC 2 cut(s) 14, 47
BalI TGGCCA 1 cut(s) 431
BbsI GAAGAC 2 cut(s) 219, 245
BbvCI CCTCAGC 3 cut(s) 282, 306, 369
BbvI GCAGC 1 cut(s) 322
BccI CCATC 2 cut(s) 260, 437
BciT130I CCWGG 1 cut(s) 356
BcnI CCSGG 1 cut(s) 161
BcoDI GTCTC 1 cut(s) 401
BfaI CTAG 1 cut(s) 321
BfoI RGCGCY 1 cut(s) 44
BisI GCNGC 5 cut(s) 173, 191, 231, 336, 743
BlsI GCNGC 5 cut(s) 174, 192, 232, 337, 744
Bme1390I CCNGG 2 cut(s) 161, 356
BmeT110I CYCGRG 1 cut(s) 243
BmiI GGNNCC 2 cut(s) 158, 450
BmrFI CCNGG 2 cut(s) 161, 356
BmsI GCATC 1 cut(s) 669
BoxI GACNNNNGTC 1 cut(s) 245
BpiI GAAGAC 2 cut(s) 219, 245
Bpu10I CCTNAGC 5 cut(s) 282, 294, 306, 369, 640
Bpu14I TTCGAA 1 cut(s) 8
BpuMI CCSGG 1 cut(s) 161
Bsa29I ATCGAT 1 cut(s) 712
Bse1I ACTGG 2 cut(s) 277, 655
BseBI CCWGG 1 cut(s) 356
BseCI ATCGAT 1 cut(s) 712
BseGI GGATG 4 cut(s) 22, 364, 379, 673
BseMII CTCAG 5 cut(s) 273, 281, 297, 360, 693
BseNI ACTGG 2 cut(s) 277, 655
BseRI GAGGAG 2 cut(s) 154, 323
BseXI GCAGC 1 cut(s) 322
BseYI CCCAGC 1 cut(s) 43
Bsh1236I CGCG 3 cut(s) 27, 29, 92
Bsh1285I CGRYCG 1 cut(s) 716
BshFI GGCC 2 cut(s) 431, 700
BshVI ATCGAT 1 cut(s) 712
BsiEI CGRYCG 1 cut(s) 716
BsiHKCI CYCGRG 1 cut(s) 243
BsiSI CCGG 1 cut(s) 160
BsmAI GTCTC 1 cut(s) 401
BsnI GGCC 2 cut(s) 431, 700
BsoBI CYCGRG 1 cut(s) 243
Bsp119I TTCGAA 1 cut(s) 8
Bsp143I GATC 5 cut(s) 87, 143, 151, 709, 713
Bsp68I TCGCGA 1 cut(s) 92
BspACI CCGC 5 cut(s) 172, 191, 230, 233, 742
BspANI GGCC 2 cut(s) 431, 700
BspCNI CTCAG 5 cut(s) 274, 280, 298, 361, 694
BspDI ATCGAT 1 cut(s) 712
BspFNI CGCG 3 cut(s) 27, 29, 92
BspLI GGNNCC 2 cut(s) 158, 450
BspPI GGATC 2 cut(s) 159, 717
BspT104I TTCGAA 1 cut(s) 8
BsrI ACTGG 2 cut(s) 277, 655
BssMI GATC 5 cut(s) 87, 143, 151, 709, 713
Bst2UI CCWGG 1 cut(s) 356
Bst4CI ACNGT 1 cut(s) 79
Bst6I CTCTTC 1 cut(s) 201
BstBI TTCGAA 1 cut(s) 8
BstC8I GCNNGC 1 cut(s) 195
BstDEI CTNAG 9 cut(s) 267, 282, 294, 306, 369, 524, 602, 640, 702
BstEII GGTNACC 2 cut(s) 247, 548
BstF5I GGATG 4 cut(s) 22, 364, 379, 673
BstFNI CGCG 3 cut(s) 27, 29, 92
BstH2I RGCGCY 1 cut(s) 44
BstHHI GCGC 3 cut(s) 29, 43, 109
BstKTI GATC 5 cut(s) 90, 146, 154, 712, 716
BstMAI GTCTC 1 cut(s) 401
BstMBI GATC 5 cut(s) 87, 143, 151, 709, 713
BstMCI CGRYCG 1 cut(s) 716
BstMWI GCNNNNNNNGC 2 cut(s) 181, 190
BstNI CCWGG 1 cut(s) 356
BstPAI GACNNNNGTC 1 cut(s) 245
BstPI GGTNACC 2 cut(s) 247, 548
BstSCI CCNGG 2 cut(s) 159, 354
BstUI CGCG 3 cut(s) 27, 29, 92
BstV1I GCAGC 1 cut(s) 322
BstV2I GAAGAC 2 cut(s) 219, 245
Bsu15I ATCGAT 1 cut(s) 712
BsuRI GGCC 2 cut(s) 431, 700
BsuTUI ATCGAT 1 cut(s) 712
BtsCI GGATG 4 cut(s) 22, 364, 379, 673
BtsIMutI CAGTG 1 cut(s) 662
BtuMI TCGCGA 1 cut(s) 92
Cac8I GCNNGC 1 cut(s) 195
CfoI GCGC 3 cut(s) 29, 43, 109
ClaI ATCGAT 1 cut(s) 712
CseI GACGC 1 cut(s) 611
Csp6I GTAC 2 cut(s) 23, 276
CviQI GTAC 2 cut(s) 23, 276
DdeI CTNAG 9 cut(s) 267, 282, 294, 306, 369, 524, 602, 640, 702
DpnI GATC 5 cut(s) 89, 145, 153, 711, 715
DpnII GATC 5 cut(s) 87, 143, 151, 709, 713
EaeI YGGCCR 1 cut(s) 429
Eam1104I CTCTTC 1 cut(s) 201
EarI CTCTTC 1 cut(s) 201
Eco57I CTGAAG 1 cut(s) 419
Eco88I CYCGRG 1 cut(s) 243
Eco91I GGTNACC 2 cut(s) 247, 548
EcoO65I GGTNACC 2 cut(s) 247, 548
EcoRI GAATTC 1 cut(s) 10
EcoRII CCWGG 1 cut(s) 354
FauI CCCGC 1 cut(s) 226
FblI GTMKAC 1 cut(s) 351
Fnu4HI GCNGC 5 cut(s) 173, 191, 231, 336, 743
FokI GGATG 4 cut(s) 29, 371, 386, 680
Fsp4HI GCNGC 5 cut(s) 173, 191, 231, 336, 743
FspBI CTAG 1 cut(s) 321
GlaI GCGC 3 cut(s) 28, 42, 108
GluI GCNGC 5 cut(s) 173, 191, 231, 336, 743
GsaI CCCAGC 1 cut(s) 47
HaeII RGCGCY 1 cut(s) 44
HaeIII GGCC 2 cut(s) 431, 700
HapII CCGG 1 cut(s) 160
HgaI GACGC 1 cut(s) 611
HhaI GCGC 3 cut(s) 29, 43, 109
Hin6I GCGC 3 cut(s) 27, 41, 107
HinP1I GCGC 3 cut(s) 27, 41, 107
HincII GTYRAC 1 cut(s) 352
HindII GTYRAC 1 cut(s) 352
HinfI GANTC 4 cut(s) 35, 241, 288, 689
HpaII CCGG 1 cut(s) 160
HphI GGTGA 6 cut(s) 79, 97, 178, 241, 518, 656
Hpy166II GTNNAC 2 cut(s) 352, 486
Hpy188I TCNGA 5 cut(s) 16, 151, 156, 270, 718
Hpy188III TCNNGA 1 cut(s) 91
Hpy8I GTNNAC 2 cut(s) 352, 486
Hpy99I CGWCG 2 cut(s) 97, 151
HpyAV CCTTC 2 cut(s) 337, 605
HpyCH4III ACNGT 1 cut(s) 79
HpyCH4V TGCA 3 cut(s) 464, 617, 660
HpyF10VI GCNNNNNNNGC 2 cut(s) 181, 190
HpyF3I CTNAG 9 cut(s) 267, 282, 294, 306, 369, 524, 602, 640, 702
HspAI GCGC 3 cut(s) 27, 41, 107
Kzo9I GATC 5 cut(s) 87, 143, 151, 709, 713
LmnI GCTCC 2 cut(s) 156, 448
Lsp1109I GCAGC 1 cut(s) 322
LweI GCATC 1 cut(s) 669
MaeI CTAG 1 cut(s) 321
MaeIII GTNAC 3 cut(s) 67, 247, 548
MalI GATC 5 cut(s) 89, 145, 153, 711, 715
MboI GATC 5 cut(s) 87, 143, 151, 709, 713
MboII GAAGA 8 cut(s) 218, 224, 247, 250, 401, 412, 620, 736
MfeI CAATTG 1 cut(s) 102
MlsI TGGCCA 1 cut(s) 431
MluCI AATT 4 cut(s) 10, 102, 114, 735
MluNI TGGCCA 1 cut(s) 431
MlyI GAGTC 2 cut(s) 44, 235
MmeI TCCRAC 3 cut(s) 12, 129, 687
Mox20I TGGCCA 1 cut(s) 431
MscI TGGCCA 1 cut(s) 431
MseI TTAA 1 cut(s) 315
MslI CAYNNNNRTG 1 cut(s) 80
Msp20I TGGCCA 1 cut(s) 431
MspA1I CMGCKG 2 cut(s) 281, 760
MspI CCGG 1 cut(s) 160
MspR9I CCNGG 2 cut(s) 161, 356
MunI CAATTG 1 cut(s) 102
MvaI CCWGG 1 cut(s) 356
MvnI CGCG 3 cut(s) 27, 29, 92
MwoI GCNNNNNNNGC 2 cut(s) 181, 190
NciI CCSGG 1 cut(s) 161
NdeII GATC 5 cut(s) 87, 143, 151, 709, 713
NlaIV GGNNCC 2 cut(s) 158, 450
NmuCI GTSAC 2 cut(s) 67, 247
NruI TCGCGA 1 cut(s) 92
NspV TTCGAA 1 cut(s) 8
OliI CACNNNNGTG 1 cut(s) 80
PfeI GAWTC 2 cut(s) 288, 689
PkrI GCNGC 5 cut(s) 174, 192, 232, 337, 744
Ple19I CGATCG 1 cut(s) 716
PleI GAGTC 2 cut(s) 43, 235
PpsI GAGTC 2 cut(s) 43, 235
PshAI GACNNNNGTC 1 cut(s) 245
Psp6I CCWGG 1 cut(s) 354
PspEI GGTNACC 2 cut(s) 247, 548
PspFI CCCAGC 1 cut(s) 43
PspGI CCWGG 1 cut(s) 354
PspN4I GGNNCC 2 cut(s) 158, 450
PvuI CGATCG 1 cut(s) 716
PvuII CAGCTG 2 cut(s) 281, 760
RruI TCGCGA 1 cut(s) 92
RsaI GTAC 2 cut(s) 24, 277
RsaNI GTAC 2 cut(s) 23, 276
RseI CAYNNNNRTG 1 cut(s) 80
SalI GTCGAC 1 cut(s) 350
SaqAI TTAA 1 cut(s) 315
SatI GCNGC 5 cut(s) 173, 191, 231, 336, 743
Sau3AI GATC 5 cut(s) 87, 143, 151, 709, 713
SchI GAGTC 2 cut(s) 44, 235
ScrFI CCNGG 2 cut(s) 161, 356
SfaNI GCATC 1 cut(s) 669
SfuI TTCGAA 1 cut(s) 8
SmiMI CAYNNNNRTG 1 cut(s) 80
Sse9I AATT 4 cut(s) 10, 102, 114, 735
SsiI CCGC 5 cut(s) 172, 191, 230, 233, 742
SspMI CTAG 1 cut(s) 321
StyD4I CCNGG 2 cut(s) 159, 354
TaaI ACNGT 1 cut(s) 79
TaqI TCGA 4 cut(s) 8, 351, 663, 712
TasI AATT 4 cut(s) 10, 102, 114, 735
TauI GCSGC 4 cut(s) 175, 193, 233, 745
TfiI GAWTC 2 cut(s) 288, 689
Tru1I TTAA 1 cut(s) 315
Tru9I TTAA 1 cut(s) 315
TscAI CASTG 1 cut(s) 662
TseFI GTSAC 2 cut(s) 67, 247
TseI GCWGC 1 cut(s) 335
Tsp45I GTSAC 2 cut(s) 67, 247
TspDTI ATGAA 3 cut(s) 402, 576, 737
TspGWI ACGGA 2 cut(s) 86, 503
TspRI CASTG 1 cut(s) 662
XapI RAATTY 2 cut(s) 10, 735
XmiI GTMKAC 1 cut(s) 351
XspI CTAG 1 cut(s) 321
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.