pycom15g34700

M-phase-specific PLK1-interacting protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0014057
Physical Location & Seq
Reverse (-)
34481765 .. 34482280
516 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 516 bp
ATGCACCAATTACAAGGTTCCTATCAAAATTTTAGTCCTCAAAGAAGCCCCGTAGGAATGGAAAGACCTTTTCCTATGCATAATGGGAATCGACCTGAAGTCTGGAATGGAGCTGAATTCACGCCACCAGGAAGCCCTCAATTGAGACCACCAGTAAGCGCTGGATATGTACCACAAGGAAGCCCGGGATTTAGACCACCAGGAAGCCCTGGATTTGGACCACCTGGAAGCCCTGGATTTAGACCAACAACAAGTCCTGGATTTAGGCCACCGACTAGCCCTGGATTTAGGCCACCAGGAAGCCGTGGTTCCAATATTGGGCAAGGCAGGGGGCACTGGTTCAGCCATAGTCGAAGACCACAAACAGTACATAGAGGCAGCCCTAGTTCTAGTTCAAGTTCAGGAAGAGGTGGTGGCGTGGTTCTCATGGTCATGCAATGGACGGACAATTGGGGCTGGAGCAGTTTTACGATGAGTCCATGGTTGAAGATCCATGGAAGTTTCTTGAACCTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000375 GO:0000377 GO:0000380 GO:0000381 GO:0000398 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0005829 GO:0005886 GO:0006139 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0006810 GO:0006950 GO:0006970 GO:0007623 GO:0008104 GO:0008150 GO:0008152 GO:0008380 GO:0009266 GO:0009409 GO:0009605 GO:0009628 GO:0009649 GO:0009651 GO:0009889 GO:0009892 GO:0009914 GO:0009926 GO:0009987 GO:0010033 GO:0010467 GO:0010468 GO:0010605 GO:0010608 GO:0010629 GO:0010817 GO:0010866 GO:0010921 GO:0014070 GO:0016020 GO:0016070 GO:0016071 GO:0016246 GO:0016441 GO:0016458 GO:0016604 GO:0019216 GO:0019219 GO:0019220 GO:0019222 GO:0030422 GO:0031047 GO:0031050 GO:0031323 GO:0031326 GO:0031974 GO:0031981 GO:0032879 GO:0033036 GO:0034641 GO:0035194 GO:0035195 GO:0035196 GO:0035303 GO:0040029 GO:0042221 GO:0042752 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043331 GO:0043484 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044451 GO:0044464 GO:0046483 GO:0046890 GO:0048024 GO:0048511 GO:0048519 GO:0050684 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051049 GO:0051171 GO:0051174 GO:0051179 GO:0051234 GO:0051252 GO:0051336 GO:0051716 GO:0060255 GO:0060918 GO:0065007 GO:0065008 GO:0065009 GO:0070013 GO:0070887 GO:0070918 GO:0071071 GO:0071310 GO:0071359 GO:0071407 GO:0071704 GO:0071944 GO:0080090 GO:0090207 GO:0090304 GO:1901360 GO:1901698 GO:1901699 GO:1901703 GO:1903311 GO:1903725 GO:1903730 GO:2000012
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MPLKIP

Protein Analysis

172

Amino Acids

18.44

Weight (kDa)

12.0

Isoelectric Point (pI)

87.68

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014057)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G24500 AT4G24500 AT4G24500
fragaria_vesca FvH4_5g34870
malus_domestica MD08G1199700.v1.1
prunus_persica Prupe.1G533200_v2.0.a1
pyrus_communis pycom08g17330 pycom15g34700
rosa_chinensis RchiOBHm_Chr7g0235791
rosa_laevigata RLG00000001139
rosa_roxburghii Rroxscaffold_3G00225630
rosa_rugosa Rorug07G0292800
rosa_samantha Rh7AG447700 Rh7BG419900 Rh7CG468200 Rh7DG436700
rosa_wichuraiana Rw7G037200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 484
AcsI RAATTY 2 cut(s) 28, 116
AcuI CTGAAG 1 cut(s) 117
AfaI GTAC 2 cut(s) 171, 369
AfeI AGCGCT 1 cut(s) 160
AfiI CCNNNNNNNGG 2 cut(s) 215, 318
AgsI TTSAA 3 cut(s) 396, 487, 508
AhdI GACNNNNNGTC 1 cut(s) 98
AjnI CCWGG 8 cut(s) 127, 199, 208, 223, 232, 256, 280, 295
AluBI AGCT 1 cut(s) 113
AluI AGCT 1 cut(s) 113
Alw26I GTCTC 1 cut(s) 139
AlwI GGATC 1 cut(s) 484
Ama87I CYCGRG 1 cut(s) 184
Aor51HI AGCGCT 1 cut(s) 160
AoxI GGCC 2 cut(s) 266, 290
ApeKI GCWGC 1 cut(s) 378
ApoI RAATTY 2 cut(s) 28, 116
ArsI GACNNNNNNTTYG 2 cut(s) 19, 51
AspLEI GCGC 1 cut(s) 161
AspS9I GGNCC 1 cut(s) 218
AsuC2I CCSGG 2 cut(s) 185, 186
AvaI CYCGRG 1 cut(s) 184
AvaII GGWCC 1 cut(s) 218
BaeGI GKGCMC 1 cut(s) 336
BbsI GAAGAC 1 cut(s) 361
BbvI GCAGC 1 cut(s) 390
BceAI ACGGC 1 cut(s) 288
BciT130I CCWGG 8 cut(s) 129, 201, 210, 225, 234, 258, 282, 297
BcnI CCSGG 2 cut(s) 185, 186
BcoDI GTCTC 1 cut(s) 139
BfaI CTAG 3 cut(s) 276, 384, 390
BfoI RGCGCY 1 cut(s) 162
BisI GCNGC 1 cut(s) 379
BlsI GCNGC 1 cut(s) 380
Bme18I GGWCC 1 cut(s) 218
BmeRI GACNNNNNGTC 1 cut(s) 98
BmeT110I CYCGRG 1 cut(s) 184
BmgT120I GGNCC 1 cut(s) 218
BmiI GGNNCC 2 cut(s) 19, 310
BpiI GAAGAC 1 cut(s) 361
BpmI CTGGAG 1 cut(s) 478
BpuMI CCSGG 2 cut(s) 185, 186
BsaI GGTCTC 1 cut(s) 139
BsaJI CCNNGG 7 cut(s) 184, 208, 232, 280, 304, 479, 493
Bsc4I CCNNNNNNNGG 2 cut(s) 215, 318
Bse1I ACTGG 2 cut(s) 152, 341
Bse3DI GCAATG 1 cut(s) 443
BseBI CCWGG 8 cut(s) 129, 201, 210, 225, 234, 258, 282, 297
BseDI CCNNGG 7 cut(s) 184, 208, 232, 280, 304, 479, 493
BseLI CCNNNNNNNGG 2 cut(s) 215, 318
BseMI GCAATG 1 cut(s) 443
BseNI ACTGG 2 cut(s) 152, 341
BseSI GKGCMC 1 cut(s) 336
BseXI GCAGC 1 cut(s) 390
BshFI GGCC 2 cut(s) 268, 292
BsiHKCI CYCGRG 1 cut(s) 184
BsiSI CCGG 1 cut(s) 185
BslI CCNNNNNNNGG 2 cut(s) 215, 318
BsmAI GTCTC 1 cut(s) 139
BsnI GGCC 2 cut(s) 268, 292
Bso31I GGTCTC 1 cut(s) 139
BsoBI CYCGRG 1 cut(s) 184
Bsp1286I GDGCHC 1 cut(s) 336
Bsp143I GATC 1 cut(s) 489
Bsp19I CCATGG 2 cut(s) 479, 493
BspANI GGCC 2 cut(s) 268, 292
BspLI GGNNCC 2 cut(s) 19, 310
BspPI GGATC 1 cut(s) 484
BspTNI GGTCTC 1 cut(s) 139
BsrDI GCAATG 1 cut(s) 443
BsrI ACTGG 2 cut(s) 152, 341
BssECI CCNNGG 7 cut(s) 184, 208, 232, 280, 304, 479, 493
BssMI GATC 1 cut(s) 489
BssT1I CCWWGG 2 cut(s) 479, 493
Bst2UI CCWGG 8 cut(s) 129, 201, 210, 225, 234, 258, 282, 297
Bst4CI ACNGT 1 cut(s) 367
Bst6I CTCTTC 1 cut(s) 400
BstDSI CCRYGG 3 cut(s) 304, 479, 493
BstH2I RGCGCY 1 cut(s) 162
BstHHI GCGC 1 cut(s) 161
BstKTI GATC 1 cut(s) 492
BstMAI GTCTC 1 cut(s) 139
BstMBI GATC 1 cut(s) 489
BstNI CCWGG 8 cut(s) 129, 201, 210, 225, 234, 258, 282, 297
BstSLI GKGCMC 1 cut(s) 336
BstV1I GCAGC 1 cut(s) 390
BstV2I GAAGAC 1 cut(s) 361
BstX2I RGATCY 1 cut(s) 489
BstYI RGATCY 1 cut(s) 489
BsuRI GGCC 2 cut(s) 268, 292
BtgI CCRYGG 3 cut(s) 304, 479, 493
BtsIMutI CAGTG 1 cut(s) 334
CfoI GCGC 1 cut(s) 161
Cfr13I GGNCC 1 cut(s) 218
Cfr9I CCCGGG 1 cut(s) 184
Csp6I GTAC 2 cut(s) 170, 368
CviAII CATG 4 cut(s) 427, 433, 480, 494
CviQI GTAC 2 cut(s) 170, 368
DpnI GATC 1 cut(s) 491
DpnII GATC 1 cut(s) 489
DriI GACNNNNNGTC 1 cut(s) 98
Eam1104I CTCTTC 1 cut(s) 400
Eam1105I GACNNNNNGTC 1 cut(s) 98
EarI CTCTTC 1 cut(s) 400
Eco130I CCWWGG 2 cut(s) 479, 493
Eco31I GGTCTC 1 cut(s) 139
Eco47I GGWCC 1 cut(s) 218
Eco47III AGCGCT 1 cut(s) 160
Eco57I CTGAAG 1 cut(s) 117
Eco88I CYCGRG 1 cut(s) 184
EcoRI GAATTC 1 cut(s) 116
EcoRII CCWGG 8 cut(s) 127, 199, 208, 223, 232, 256, 280, 295
EcoT14I CCWWGG 2 cut(s) 479, 493
EcoT22I ATGCAT 1 cut(s) 81
ErhI CCWWGG 2 cut(s) 479, 493
FaeI CATG 4 cut(s) 430, 436, 483, 497
FaiI YATR 9 cut(s) 77, 81, 168, 348, 372, 428, 434, 481, 495
FatI CATG 4 cut(s) 426, 432, 479, 493
Fnu4HI GCNGC 1 cut(s) 379
Fsp4HI GCNGC 1 cut(s) 379
FspBI CTAG 3 cut(s) 276, 384, 390
GlaI GCGC 1 cut(s) 160
GluI GCNGC 1 cut(s) 379
GsuI CTGGAG 1 cut(s) 478
HaeII RGCGCY 1 cut(s) 162
HaeIII GGCC 2 cut(s) 268, 292
HapII CCGG 1 cut(s) 185
HhaI GCGC 1 cut(s) 161
Hin1II CATG 4 cut(s) 430, 436, 483, 497
Hin6I GCGC 1 cut(s) 159
HinP1I GCGC 1 cut(s) 159
HinfI GANTC 2 cut(s) 88, 475
HpaII CCGG 1 cut(s) 185
Hpy188III TCNNGA 3 cut(s) 103, 402, 505
HpyCH4III ACNGT 1 cut(s) 367
HpyCH4V TGCA 3 cut(s) 4, 79, 436
Hsp92II CATG 4 cut(s) 430, 436, 483, 497
HspAI GCGC 1 cut(s) 159
Kzo9I GATC 1 cut(s) 489
LmnI GCTCC 2 cut(s) 110, 459
Lsp1109I GCAGC 1 cut(s) 390
MaeI CTAG 3 cut(s) 276, 384, 390
MalI GATC 1 cut(s) 491
MboI GATC 1 cut(s) 489
MboII GAAGA 3 cut(s) 366, 417, 499
MfeI CAATTG 2 cut(s) 140, 448
MflI RGATCY 1 cut(s) 489
MhlI GDGCHC 1 cut(s) 336
MluCI AATT 5 cut(s) 8, 28, 116, 140, 448
MlyI GAGTC 1 cut(s) 484
MnlI CCTC 4 cut(s) 48, 147, 368, 401
Mph1103I ATGCAT 1 cut(s) 81
MslI CAYNNNNRTG 1 cut(s) 431
MspI CCGG 1 cut(s) 185
MunI CAATTG 2 cut(s) 140, 448
MvaI CCWGG 8 cut(s) 129, 201, 210, 225, 234, 258, 282, 297
NciI CCSGG 2 cut(s) 185, 186
NcoI CCATGG 2 cut(s) 479, 493
NdeII GATC 1 cut(s) 489
NlaIII CATG 4 cut(s) 430, 436, 483, 497
NlaIV GGNNCC 2 cut(s) 19, 310
NsiI ATGCAT 1 cut(s) 81
PfeI GAWTC 1 cut(s) 88
PfoI TCCNGGA 1 cut(s) 256
PkrI GCNGC 1 cut(s) 380
PleI GAGTC 1 cut(s) 483
PpsI GAGTC 1 cut(s) 483
Psp6I CCWGG 8 cut(s) 127, 199, 208, 223, 232, 256, 280, 295
PspGI CCWGG 8 cut(s) 127, 199, 208, 223, 232, 256, 280, 295
PspN4I GGNNCC 2 cut(s) 19, 310
PspPI GGNCC 1 cut(s) 218
PsuI RGATCY 1 cut(s) 489
RsaI GTAC 2 cut(s) 171, 369
RsaNI GTAC 2 cut(s) 170, 368
RseI CAYNNNNRTG 1 cut(s) 431
SatI GCNGC 1 cut(s) 379
Sau3AI GATC 1 cut(s) 489
Sau96I GGNCC 1 cut(s) 218
SchI GAGTC 1 cut(s) 484
SduI GDGCHC 1 cut(s) 336
SetI ASST 7 cut(s) 19, 70, 97, 115, 226, 412, 513
SinI GGWCC 1 cut(s) 218
SmaI CCCGGG 1 cut(s) 186
SmiMI CAYNNNNRTG 1 cut(s) 431
Sse9I AATT 5 cut(s) 8, 28, 116, 140, 448
SspI AATATT 1 cut(s) 316
SspMI CTAG 3 cut(s) 276, 384, 390
StyI CCWWGG 2 cut(s) 479, 493
TaaI ACNGT 1 cut(s) 367
TaqI TCGA 2 cut(s) 91, 352
TasI AATT 5 cut(s) 8, 28, 116, 140, 448
TatI WGTACW 1 cut(s) 367
TfiI GAWTC 1 cut(s) 88
TscAI CASTG 1 cut(s) 341
TseI GCWGC 1 cut(s) 378
TspGWI ACGGA 1 cut(s) 458
TspMI CCCGGG 1 cut(s) 184
TspRI CASTG 1 cut(s) 341
VpaK11BI GGWCC 1 cut(s) 218
XapI RAATTY 2 cut(s) 28, 116
XmaI CCCGGG 1 cut(s) 184
XspI CTAG 3 cut(s) 276, 384, 390
Zsp2I ATGCAT 1 cut(s) 81
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.