pycom15g35350

E2-like enzyme which forms an intermediate with UFM1 via a thioester linkage

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0011875
Physical Location & Seq
Reverse (-)
35278872 .. 35280133
1262 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 387 bp
ATGAACAAGTCCAACGACAACGATTGGTTCCGAATCTCCGCCTCCAATCCCGAGGGTACACGGTGGACTGGCAAGTGCTGGTACGTCTACAACCTCCTCAAGTACGAGTTCGATCTCCAATTCGATATCCCCATCACCTACCCCGCCACCGCACCGGAACTCGAGCTCCCTCAGCTAGACGGCAAGACCCAAAAGATGTATAGAGGGGGGAAGATCTGCTTGACCGTGCATTTCAAGCCGCTTTGGGCCAAGAACTGTCCAAGATTTGGTATAGCACATGCACTTTGTTTGGGTCTTGCACCATGGCTTGCGGCAGAGATTCCCGTTCTTGTGGATTCTGGGATGGTTAAGCACAAAGATGATGCTGCATCATCAACTGAATCTTAG

Protein Analysis

129

Amino Acids

14.46

Weight (kDa)

6.81

Isoelectric Point (pI)

39.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UFC1 PF08694 1 - 117 1.3e-65 Ubiquitin-fold modifier-conjugating enzyme 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011875)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G27530
fragaria_vesca FvH4_5g33720 FvH4_5g33720
malus_domestica MD05G1007500.v1.1 MD08G1209200.v1.1 MD15G1393200.v1.1
prunus_persica Prupe.1G541400_v2.0.a1 Prupe.1G541400_v2.0.a1 Prupe.1G541400_v2.0.a1
pyrus_communis pycom05g10270 pycom08g18060 pycom15g35350
rosa_chinensis RchiOBHm_Chr7g0232851
rosa_laevigata RLG00000001313
rosa_multiflora Rmu_ssc0000018.1_g000017
rosa_roxburghii Rroxscaffold_3G00227850
rosa_rugosa Rorug07G0276400
rosa_samantha Rh7CG450300
rosa_wichuraiana Rw7G035630

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 266
AccI GTMKAC 1 cut(s) 87
AciI CCGC 5 cut(s) 39, 144, 150, 239, 311
AfaI GTAC 3 cut(s) 58, 83, 104
AfiI CCNNNNNNNGG 1 cut(s) 266
AgsI TTSAA 1 cut(s) 235
AloI GAACNNNNNNTCC 2 cut(s) 150, 182
AluBI AGCT 2 cut(s) 166, 175
AluI AGCT 2 cut(s) 166, 175
Alw21I GWGCWC 1 cut(s) 168
Ama87I CYCGRG 2 cut(s) 50, 161
AoxI GGCC 1 cut(s) 246
ApeKI GCWGC 1 cut(s) 365
AspS9I GGNCC 1 cut(s) 246
AsuHPI GGTGA 1 cut(s) 127
AvaI CYCGRG 2 cut(s) 50, 161
BanII GRGCYC 1 cut(s) 168
Bbv12I GWGCWC 1 cut(s) 168
BbvCI CCTCAGC 1 cut(s) 171
BbvI GCAGC 1 cut(s) 352
BccI CCATC 2 cut(s) 140, 337
BceAI ACGGC 1 cut(s) 196
BfaI CTAG 1 cut(s) 176
BglII AGATCT 1 cut(s) 213
BisI GCNGC 3 cut(s) 239, 312, 366
BlsI GCNGC 3 cut(s) 240, 313, 367
BmeT110I CYCGRG 2 cut(s) 50, 161
BmgT120I GGNCC 1 cut(s) 246
BmiI GGNNCC 1 cut(s) 29
BmsI GCATC 2 cut(s) 352, 377
Bpu10I CCTNAGC 1 cut(s) 171
BpuEI CTTGAG 1 cut(s) 83
BsaJI CCNNGG 2 cut(s) 51, 302
BsaWI WCCGGW 1 cut(s) 154
BsaXI ACNNNNNCTCC 2 cut(s) 150, 180
Bsc4I CCNNNNNNNGG 1 cut(s) 266
Bse1I ACTGG 1 cut(s) 73
BseDI CCNNGG 2 cut(s) 51, 302
BseGI GGATG 1 cut(s) 348
BseLI CCNNNNNNNGG 1 cut(s) 266
BseMII CTCAG 1 cut(s) 185
BseNI ACTGG 1 cut(s) 73
BseRI GAGGAG 1 cut(s) 86
BseXI GCAGC 1 cut(s) 352
BshFI GGCC 1 cut(s) 248
BsiHKAI GWGCWC 1 cut(s) 168
BsiHKCI CYCGRG 2 cut(s) 50, 161
BsiSI CCGG 1 cut(s) 155
BslI CCNNNNNNNGG 1 cut(s) 266
BsnI GGCC 1 cut(s) 248
BsoBI CYCGRG 2 cut(s) 50, 161
Bsp1286I GDGCHC 1 cut(s) 168
Bsp143I GATC 2 cut(s) 112, 213
Bsp19I CCATGG 1 cut(s) 302
BspACI CCGC 5 cut(s) 39, 144, 150, 239, 311
BspANI GGCC 1 cut(s) 248
BspCNI CTCAG 1 cut(s) 184
BspLI GGNNCC 1 cut(s) 29
BsrI ACTGG 1 cut(s) 73
BssECI CCNNGG 2 cut(s) 51, 302
BssMI GATC 2 cut(s) 112, 213
BssT1I CCWWGG 1 cut(s) 302
Bst4CI ACNGT 3 cut(s) 63, 226, 257
BstC8I GCNNGC 1 cut(s) 309
BstDEI CTNAG 2 cut(s) 171, 384
BstDSI CCRYGG 1 cut(s) 302
BstF5I GGATG 1 cut(s) 348
BstKTI GATC 2 cut(s) 115, 216
BstMBI GATC 2 cut(s) 112, 213
BstMWI GCNNNNNNNGC 2 cut(s) 172, 235
BstNSI RCATGY 1 cut(s) 281
BstV1I GCAGC 1 cut(s) 352
BstX2I RGATCY 1 cut(s) 213
BstYI RGATCY 1 cut(s) 213
BsuRI GGCC 1 cut(s) 248
BtgI CCRYGG 1 cut(s) 302
BtsCI GGATG 1 cut(s) 348
Cac8I GCNNGC 1 cut(s) 309
Cfr13I GGNCC 1 cut(s) 246
Csp6I GTAC 3 cut(s) 57, 82, 103
CviAII CATG 2 cut(s) 278, 303
CviJI RGCY 5 cut(s) 166, 175, 238, 248, 307
CviKI_1 RGCY 5 cut(s) 166, 175, 238, 248, 307
CviQI GTAC 3 cut(s) 57, 82, 103
DdeI CTNAG 2 cut(s) 171, 384
DpnI GATC 2 cut(s) 114, 215
DpnII GATC 2 cut(s) 112, 213
EciI GGCGGA 1 cut(s) 28
Ecl136II GAGCTC 1 cut(s) 166
Eco130I CCWWGG 1 cut(s) 302
Eco24I GRGCYC 1 cut(s) 168
Eco32I GATATC 1 cut(s) 127
Eco53kI GAGCTC 1 cut(s) 166
Eco88I CYCGRG 2 cut(s) 50, 161
EcoICRI GAGCTC 1 cut(s) 166
EcoRV GATATC 1 cut(s) 127
EcoT14I CCWWGG 1 cut(s) 302
EcoT38I GRGCYC 1 cut(s) 168
ErhI CCWWGG 1 cut(s) 302
FaeI CATG 2 cut(s) 281, 306
FaiI YATR 4 cut(s) 201, 272, 279, 304
FalI AAGNNNNNCTT 2 cut(s) 203, 235
FatI CATG 2 cut(s) 277, 302
FauI CCCGC 1 cut(s) 151
FblI GTMKAC 1 cut(s) 87
Fnu4HI GCNGC 3 cut(s) 239, 312, 366
FokI GGATG 1 cut(s) 355
FriOI GRGCYC 1 cut(s) 168
Fsp4HI GCNGC 3 cut(s) 239, 312, 366
FspBI CTAG 1 cut(s) 176
GluI GCNGC 3 cut(s) 239, 312, 366
HaeIII GGCC 1 cut(s) 248
HapII CCGG 1 cut(s) 155
Hin1II CATG 2 cut(s) 281, 306
HinfI GANTC 4 cut(s) 33, 319, 335, 380
HpaII CCGG 1 cut(s) 155
HphI GGTGA 1 cut(s) 127
Hpy166II GTNNAC 3 cut(s) 59, 66, 88
Hpy188I TCNGA 1 cut(s) 32
Hpy188III TCNNGA 1 cut(s) 50
Hpy8I GTNNAC 3 cut(s) 59, 66, 88
HpyCH4III ACNGT 3 cut(s) 63, 226, 257
HpyCH4IV ACGT 1 cut(s) 84
HpyCH4V TGCA 4 cut(s) 229, 281, 299, 368
HpyF10VI GCNNNNNNNGC 2 cut(s) 172, 235
HpyF3I CTNAG 2 cut(s) 171, 384
HpySE526I ACGT 1 cut(s) 84
Hsp92II CATG 2 cut(s) 281, 306
Kzo9I GATC 2 cut(s) 112, 213
LmnI GCTCC 1 cut(s) 171
LpnPI CCDG 4 cut(s) 54, 64, 168, 324
Lsp1109I GCAGC 1 cut(s) 352
LweI GCATC 2 cut(s) 352, 377
MaeI CTAG 1 cut(s) 176
MaeII ACGT 1 cut(s) 84
MalI GATC 2 cut(s) 114, 215
MboI GATC 2 cut(s) 112, 213
MboII GAAGA 1 cut(s) 223
MflI RGATCY 1 cut(s) 213
MhlI GDGCHC 1 cut(s) 168
MluCI AATT 1 cut(s) 119
MmeI TCCRAC 1 cut(s) 36
MnlI CCTC 6 cut(s) 46, 52, 104, 107, 180, 197
MseI TTAA 1 cut(s) 348
MslI CAYNNNNRTG 1 cut(s) 357
MspI CCGG 1 cut(s) 155
MwoI GCNNNNNNNGC 2 cut(s) 172, 235
NcoI CCATGG 1 cut(s) 302
NdeII GATC 2 cut(s) 112, 213
NlaIII CATG 2 cut(s) 281, 306
NlaIV GGNNCC 1 cut(s) 29
NspI RCATGY 1 cut(s) 281
PaeR7I CTCGAG 1 cut(s) 161
PfeI GAWTC 4 cut(s) 33, 319, 335, 380
PflMI CCANNNNNTGG 1 cut(s) 266
PkrI GCNGC 3 cut(s) 240, 313, 367
Psp124BI GAGCTC 1 cut(s) 168
PspN4I GGNNCC 1 cut(s) 29
PspPI GGNCC 1 cut(s) 246
PspXI VCTCGAGB 1 cut(s) 161
PsuI RGATCY 1 cut(s) 213
RsaI GTAC 3 cut(s) 58, 83, 104
RsaNI GTAC 3 cut(s) 57, 82, 103
RseI CAYNNNNRTG 1 cut(s) 357
SacI GAGCTC 1 cut(s) 168
SaqAI TTAA 1 cut(s) 348
SatI GCNGC 3 cut(s) 239, 312, 366
Sau3AI GATC 2 cut(s) 112, 213
Sau96I GGNCC 1 cut(s) 246
SduI GDGCHC 1 cut(s) 168
SetI ASST 5 cut(s) 87, 96, 140, 168, 177
SfaNI GCATC 2 cut(s) 352, 377
Sfr274I CTCGAG 1 cut(s) 161
SlaI CTCGAG 1 cut(s) 161
SmiMI CAYNNNNRTG 1 cut(s) 357
SmlI CTYRAG 2 cut(s) 98, 161
SmoI CTYRAG 2 cut(s) 98, 161
Sse9I AATT 1 cut(s) 119
SsiI CCGC 5 cut(s) 39, 144, 150, 239, 311
SspMI CTAG 1 cut(s) 176
SstI GAGCTC 1 cut(s) 168
StyI CCWWGG 1 cut(s) 302
TaaI ACNGT 3 cut(s) 63, 226, 257
TaiI ACGT 1 cut(s) 87
TaqI TCGA 3 cut(s) 111, 123, 162
TasI AATT 1 cut(s) 119
TauI GCSGC 2 cut(s) 241, 314
TfiI GAWTC 4 cut(s) 33, 319, 335, 380
Tru1I TTAA 1 cut(s) 348
Tru9I TTAA 1 cut(s) 348
TseI GCWGC 1 cut(s) 365
TspDTI ATGAA 1 cut(s) 17
Van91I CCANNNNNTGG 1 cut(s) 266
XceI RCATGY 1 cut(s) 281
XhoI CTCGAG 1 cut(s) 161
XmiI GTMKAC 1 cut(s) 87
XspI CTAG 1 cut(s) 176
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.