pycom15g36250

ATP-dependent protease La (LON) substrate-binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0003413
Physical Location & Seq
Forward (+)
36289203 .. 36293153
3951 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 927 bp
ATGAATTGTACGGCCTTCAAATTCAATCCCACCACTCCGTTTGTCCTGGACAAGCTCGCTCAGTTTATACCAGCGACACGAATCCGAAACCCGCACCGGAATGTCTATAGTTCCGTTGGTTTCAGTTTCACCAGGAAGAAACTCAAACGATGCCGCCACTCGGCTCCTCCAAACGCCACCTCCTTGGTTGAATTGGAGCTCCCGCTCCTTCCCTTTCCCTTGAACGAGGTTCTAGTTCCCTCTGAGAGTAAGGCATTGCACTTATATGAAGCAAGGTATCTAGCTCTACTGGAGGAGTCGTTGAAAAGGAAGAAGCTTTTTGTGCATTTTGTGTTGGATCCTATCATCATTGACAATTCATCAGGAGAAGCATCCTTTGCGGCCAGAAATGGTTGCTTGGTTTTCATAGAAAATGTTGAGCGTTTAGAAGTTGGAGCACTAGTCTCTATTAGAGGAATTGGTCGTGTAAAAATTGTGAAATTTGTGCAGGCAGATCCTTACTTGAAAGGTGTTGTCGTACCTGTGCACGACAGGGTTCCTGAAGGCGAGAGCGGATTAGAACCAAAAGTTATGCAAGTAAAGGAAGCTCTATATAGTTTAAATAGCTTGGAAATCAAGCTGAAGGCCCCCAAGGAGGCACCGTTGCAGACTCGAATTGCCAATTCTCTAATGTGGAGTGAAAAGGAACTAGTCTTAGATTGTGATGAAGCTTTCATTCCTTCCTTGCCCGAGCGTGTTTCATTTGCAGCACTTCAACCGGTATCAGGATCAGGTGAATCGGAACTTGCGAAGCTACAGCTAGAGAAGTTAAGGGCAATGGAGCTCAAAGACACCGTCCAGAGGCTAGATAATTCCCTGGAATTTGTTAAAGATAATATCTCCATGGTTGCAGCCAAGCTCGCTATACAGTCAGTGGAGATGGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LON_substr_bdg

Protein Analysis

309

Amino Acids

34.42

Weight (kDa)

8.25

Isoelectric Point (pI)

33.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LON_substr_bdg PF02190 67 - 201 5.1e-12 ATP-dependent protease La (LON) substrate-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 637
AccBSI CCGCTC 2 cut(s) 205, 552
AciI CCGC 5 cut(s) 92, 154, 203, 380, 552
AclWI GGATC 4 cut(s) 332, 345, 488, 775
AcoI YGGCCR 1 cut(s) 381
AcsI RAATTY 3 cut(s) 20, 479, 860
AcuI CTGAAG 2 cut(s) 561, 641
AfaI GTAC 2 cut(s) 10, 519
AfiI CCNNNNNNNGG 4 cut(s) 160, 634, 764, 840
AgeI ACCGGT 1 cut(s) 757
AgsI TTSAA 7 cut(s) 19, 25, 191, 223, 304, 505, 755
AhlI ACTAGT 2 cut(s) 439, 688
AjnI CCWGG 3 cut(s) 45, 131, 855
Alw21I GWGCWC 4 cut(s) 201, 439, 528, 825
Alw26I GTCTC 1 cut(s) 448
Alw44I GTGCAC 1 cut(s) 524
AlwI GGATC 4 cut(s) 332, 345, 488, 775
Ama87I CYCGRG 1 cut(s) 728
AoxI GGCC 3 cut(s) 12, 381, 624
ApaLI GTGCAC 1 cut(s) 524
ApeKI GCWGC 2 cut(s) 746, 890
ApoI RAATTY 3 cut(s) 20, 479, 860
ArsI GACNNNNNNTTYG 2 cut(s) 819, 851
AsiGI ACCGGT 1 cut(s) 757
AspS9I GGNCC 1 cut(s) 625
AsuHPI GGTGA 2 cut(s) 121, 785
AvaI CYCGRG 1 cut(s) 728
BaeGI GKGCMC 1 cut(s) 528
BamHI GGATCC 1 cut(s) 337
BanI GGYRCC 1 cut(s) 637
BanII GRGCYC 2 cut(s) 201, 825
Bbv12I GWGCWC 4 cut(s) 201, 439, 528, 825
BbvI GCAGC 2 cut(s) 758, 902
BccI CCATC 1 cut(s) 913
BceAI ACGGC 1 cut(s) 27
BciT130I CCWGG 3 cut(s) 47, 133, 857
BcoDI GTCTC 1 cut(s) 448
BcuI ACTAGT 2 cut(s) 439, 688
BfaI CTAG 6 cut(s) 233, 281, 440, 689, 800, 845
BfmI CTRYAG 2 cut(s) 106, 794
BisI GCNGC 4 cut(s) 154, 381, 747, 891
BlsI GCNGC 4 cut(s) 155, 382, 748, 892
Bme1390I CCNGG 3 cut(s) 47, 133, 857
BmeT110I CYCGRG 1 cut(s) 728
BmgT120I GGNCC 1 cut(s) 625
BmiI GGNNCC 5 cut(s) 165, 339, 537, 627, 639
BmrFI CCNGG 3 cut(s) 47, 133, 857
BmsI GCATC 2 cut(s) 140, 380
BpmI CTGGAG 1 cut(s) 311
BsaJI CCNNGG 4 cut(s) 183, 630, 855, 882
BsaWI WCCGGW 2 cut(s) 96, 757
BsaXI ACNNNNNCTCC 9 cut(s) 164, 194, 284, 314, 426, 456, 626, 656, 908
Bsc4I CCNNNNNNNGG 4 cut(s) 160, 634, 764, 840
Bse118I RCCGGY 1 cut(s) 757
Bse1I ACTGG 1 cut(s) 294
Bse3DI GCAATG 2 cut(s) 254, 822
BseBI CCWGG 3 cut(s) 47, 133, 857
BseDI CCNNGG 4 cut(s) 183, 630, 855, 882
BseGI GGATG 1 cut(s) 371
BseLI CCNNNNNNNGG 4 cut(s) 160, 634, 764, 840
BseMI GCAATG 2 cut(s) 254, 822
BseMII CTCAG 2 cut(s) 74, 234
BseNI ACTGG 1 cut(s) 294
BseRI GAGGAG 2 cut(s) 156, 308
BseSI GKGCMC 1 cut(s) 528
BseXI GCAGC 2 cut(s) 758, 902
BsgI GTGCAG 1 cut(s) 506
BshFI GGCC 3 cut(s) 14, 383, 626
BshNI GGYRCC 1 cut(s) 637
BshTI ACCGGT 1 cut(s) 757
BsiHKAI GWGCWC 4 cut(s) 201, 439, 528, 825
BsiHKCI CYCGRG 1 cut(s) 728
BsiSI CCGG 2 cut(s) 97, 758
BslI CCNNNNNNNGG 4 cut(s) 160, 634, 764, 840
BsmAI GTCTC 1 cut(s) 448
BsnI GGCC 3 cut(s) 14, 383, 626
BsoBI CYCGRG 1 cut(s) 728
Bsp1286I GDGCHC 4 cut(s) 201, 439, 528, 825
Bsp143I GATC 3 cut(s) 337, 493, 767
Bsp19I CCATGG 1 cut(s) 882
BspACI CCGC 5 cut(s) 92, 154, 203, 380, 552
BspANI GGCC 3 cut(s) 14, 383, 626
BspCNI CTCAG 2 cut(s) 73, 235
BspLI GGNNCC 5 cut(s) 165, 339, 537, 627, 639
BspPI GGATC 4 cut(s) 332, 345, 488, 775
BspT107I GGYRCC 1 cut(s) 637
BsrBI CCGCTC 2 cut(s) 205, 552
BsrDI GCAATG 2 cut(s) 254, 822
BsrFI RCCGGY 1 cut(s) 757
BsrI ACTGG 1 cut(s) 294
BssAI RCCGGY 1 cut(s) 757
BssECI CCNNGG 4 cut(s) 183, 630, 855, 882
BssMI GATC 3 cut(s) 337, 493, 767
BssT1I CCWWGG 3 cut(s) 183, 630, 882
Bst2UI CCWGG 3 cut(s) 47, 133, 857
Bst4CI ACNGT 3 cut(s) 642, 835, 909
BstAPI GCANNNNNTGC 1 cut(s) 377
BstC8I GCNNGC 3 cut(s) 57, 489, 900
BstDEI CTNAG 3 cut(s) 60, 243, 694
BstDSI CCRYGG 1 cut(s) 882
BstF5I GGATG 1 cut(s) 371
BstKTI GATC 3 cut(s) 340, 496, 770
BstMAI GTCTC 1 cut(s) 448
BstMBI GATC 3 cut(s) 337, 493, 767
BstMWI GCNNNNNNNGC 3 cut(s) 322, 377, 899
BstNI CCWGG 3 cut(s) 47, 133, 857
BstSCI CCNGG 3 cut(s) 45, 131, 855
BstSFI CTRYAG 2 cut(s) 106, 794
BstSLI GKGCMC 1 cut(s) 528
BstV1I GCAGC 2 cut(s) 758, 902
BstX2I RGATCY 2 cut(s) 337, 493
BstXI CCANNNNNNTGG 1 cut(s) 184
BstYI RGATCY 2 cut(s) 337, 493
BsuRI GGCC 3 cut(s) 14, 383, 626
BtgI CCRYGG 1 cut(s) 882
BtsCI GGATG 1 cut(s) 371
BtsIMutI CAGTG 1 cut(s) 918
Cac8I GCNNGC 3 cut(s) 57, 489, 900
Cfr10I RCCGGY 1 cut(s) 757
Cfr13I GGNCC 1 cut(s) 625
Csp6I GTAC 2 cut(s) 9, 518
CspAI ACCGGT 1 cut(s) 757
CspCI CAANNNNNGTGG 2 cut(s) 22, 57
CviAII CATG 1 cut(s) 883
CviQI GTAC 2 cut(s) 9, 518
DdeI CTNAG 3 cut(s) 60, 243, 694
DpnI GATC 3 cut(s) 339, 495, 769
DpnII GATC 3 cut(s) 337, 493, 767
DraI TTTAAA 1 cut(s) 600
EaeI YGGCCR 1 cut(s) 381
Ecl136II GAGCTC 2 cut(s) 199, 823
Eco130I CCWWGG 3 cut(s) 183, 630, 882
Eco24I GRGCYC 2 cut(s) 201, 825
Eco53kI GAGCTC 2 cut(s) 199, 823
Eco57I CTGAAG 2 cut(s) 561, 641
Eco88I CYCGRG 1 cut(s) 728
EcoICRI GAGCTC 2 cut(s) 199, 823
EcoO109I RGGNCCY 1 cut(s) 625
EcoRII CCWGG 3 cut(s) 45, 131, 855
EcoT14I CCWWGG 3 cut(s) 183, 630, 882
EcoT38I GRGCYC 2 cut(s) 201, 825
ErhI CCWWGG 3 cut(s) 183, 630, 882
FaeI CATG 1 cut(s) 886
FatI CATG 1 cut(s) 882
FauI CCCGC 2 cut(s) 99, 210
Fnu4HI GCNGC 4 cut(s) 154, 381, 747, 891
FokI GGATG 1 cut(s) 358
FriOI GRGCYC 2 cut(s) 201, 825
Fsp4HI GCNGC 4 cut(s) 154, 381, 747, 891
FspBI CTAG 6 cut(s) 233, 281, 440, 689, 800, 845
GluI GCNGC 4 cut(s) 154, 381, 747, 891
GsuI CTGGAG 1 cut(s) 311
HaeIII GGCC 3 cut(s) 14, 383, 626
HapII CCGG 2 cut(s) 97, 758
Hin1II CATG 1 cut(s) 886
HindIII AAGCTT 2 cut(s) 314, 708
HinfI GANTC 4 cut(s) 81, 296, 649, 776
HpaII CCGG 2 cut(s) 97, 758
HphI GGTGA 2 cut(s) 121, 785
Hpy166II GTNNAC 1 cut(s) 526
Hpy188I TCNGA 3 cut(s) 86, 244, 781
Hpy188III TCNNGA 4 cut(s) 363, 539, 765, 838
Hpy8I GTNNAC 1 cut(s) 526
HpyAV CCTTC 5 cut(s) 25, 218, 536, 616, 729
HpyCH4III ACNGT 3 cut(s) 642, 835, 909
HpyCH4V TGCA 8 cut(s) 259, 325, 487, 526, 574, 646, 746, 890
HpyF10VI GCNNNNNNNGC 3 cut(s) 322, 377, 899
HpyF3I CTNAG 3 cut(s) 60, 243, 694
Hsp92II CATG 1 cut(s) 886
Kzo9I GATC 3 cut(s) 337, 493, 767
LmnI GCTCC 6 cut(s) 169, 196, 204, 210, 434, 820
Lsp1109I GCAGC 2 cut(s) 758, 902
LweI GCATC 2 cut(s) 140, 380
MaeI CTAG 6 cut(s) 233, 281, 440, 689, 800, 845
MalI GATC 3 cut(s) 339, 495, 769
MbiI CCGCTC 2 cut(s) 205, 552
MboI GATC 3 cut(s) 337, 493, 767
MboII GAAGA 2 cut(s) 148, 322
MflI RGATCY 2 cut(s) 337, 493
MhlI GDGCHC 4 cut(s) 201, 439, 528, 825
MlyI GAGTC 2 cut(s) 305, 643
MmeI TCCRAC 2 cut(s) 315, 412
MnlI CCTC 8 cut(s) 177, 190, 220, 250, 286, 446, 628, 834
MseI TTAA 3 cut(s) 599, 809, 867
MslI CAYNNNNRTG 2 cut(s) 99, 264
MspI CCGG 2 cut(s) 97, 758
MspR9I CCNGG 3 cut(s) 47, 133, 857
MvaI CCWGG 3 cut(s) 47, 133, 857
MwoI GCNNNNNNNGC 3 cut(s) 322, 377, 899
NcoI CCATGG 1 cut(s) 882
NdeII GATC 3 cut(s) 337, 493, 767
NlaIII CATG 1 cut(s) 886
NlaIV GGNNCC 5 cut(s) 165, 339, 537, 627, 639
NmeAIII GCCGAG 1 cut(s) 140
PcsI WCGNNNNNNNCGW 1 cut(s) 785
PfeI GAWTC 2 cut(s) 81, 776
PflFI GACNNNGTC 1 cut(s) 833
PfoI TCCNGGA 1 cut(s) 45
PinAI ACCGGT 1 cut(s) 757
PkrI GCNGC 4 cut(s) 155, 382, 748, 892
PleI GAGTC 2 cut(s) 304, 643
PpsI GAGTC 2 cut(s) 304, 643
Psp124BI GAGCTC 2 cut(s) 201, 825
Psp6I CCWGG 3 cut(s) 45, 131, 855
PspGI CCWGG 3 cut(s) 45, 131, 855
PspN4I GGNNCC 5 cut(s) 165, 339, 537, 627, 639
PspPI GGNCC 1 cut(s) 625
PsuI RGATCY 2 cut(s) 337, 493
PsyI GACNNNGTC 1 cut(s) 833
RsaI GTAC 2 cut(s) 10, 519
RsaNI GTAC 2 cut(s) 9, 518
RseI CAYNNNNRTG 2 cut(s) 99, 264
SacI GAGCTC 2 cut(s) 201, 825
SaqAI TTAA 3 cut(s) 599, 809, 867
SatI GCNGC 4 cut(s) 154, 381, 747, 891
Sau3AI GATC 3 cut(s) 337, 493, 767
Sau96I GGNCC 1 cut(s) 625
SchI GAGTC 2 cut(s) 305, 643
ScrFI CCNGG 3 cut(s) 47, 133, 857
SduI GDGCHC 4 cut(s) 201, 439, 528, 825
SfaNI GCATC 2 cut(s) 140, 380
SfcI CTRYAG 2 cut(s) 106, 794
SmiMI CAYNNNNRTG 2 cut(s) 99, 264
SpeI ACTAGT 2 cut(s) 439, 688
SsiI CCGC 5 cut(s) 92, 154, 203, 380, 552
SspMI CTAG 6 cut(s) 233, 281, 440, 689, 800, 845
SstI GAGCTC 2 cut(s) 201, 825
StyD4I CCNGG 3 cut(s) 45, 131, 855
StyI CCWWGG 3 cut(s) 183, 630, 882
TaaI ACNGT 3 cut(s) 642, 835, 909
TaqI TCGA 1 cut(s) 652
TauI GCSGC 2 cut(s) 156, 383
TfiI GAWTC 2 cut(s) 81, 776
Tru1I TTAA 3 cut(s) 599, 809, 867
Tru9I TTAA 3 cut(s) 599, 809, 867
TscAI CASTG 1 cut(s) 918
TseI GCWGC 2 cut(s) 746, 890
TspDTI ATGAA 7 cut(s) 17, 282, 348, 394, 703, 720, 729
TspGWI ACGGA 2 cut(s) 27, 103
TspRI CASTG 1 cut(s) 918
Tth111I GACNNNGTC 1 cut(s) 833
VneI GTGCAC 1 cut(s) 524
XapI RAATTY 3 cut(s) 20, 479, 860
XspI CTAG 6 cut(s) 233, 281, 440, 689, 800, 845
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.