pycom15g36680

Heavy-metal-associated domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0014749
Physical Location & Seq
Reverse (-)
36644138 .. 36646396
2259 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 765 bp
ATGGTGAAGAAGGCTAGTACTAGAAATAAAATTAACTGTCTGGCAGAGCCAGTTCTCCCTCCCTCCCTCCCTCCCTCCCTCCTACTAATTAGCGACCAATTATATATCATGAACATGAAAAGCCGCAGCGGTACTAAGGAAAAGATGAAGAAGAAGATGATGATGAGAGGGTTCATGTGCCAGTCTCAGGTAGCAACTGCGGTGTGTATGTCTGATGCTCGCTCCGTTATTGTGCCGCCAACAAGACCAGCTGCAGCTACCACTGTCCGCCACAATCACAATGCTGGACATGGGCATTACATGATGATGAGAAGATCAACTAGTACTAATAATGCTGGTGATGGCAGCGGTGGTGGTCAATACTACTACTCCAGGCTCCTCAACACTCCTCCTCCTCCAGCTCATGGCCATGAGACGTCCTACCCTCACAGTCGCAGGCGAAGCACTACCACTAGCAGCATTCTTAACCTACATGATCAGCAGCCAATGTCAAAGTCGATCGGAAAACTACCATCATCTGATCCAGATTTACTACCGCAGTCGTCATCCGCGGACAATGTTTTCCAGGTGGTGGTTATGAGAGTTTCCATTCATTGCCAAGGATGTGCTGGTAAACTGAAGAAACATCTCTCTAAGATGGAAGGGGTTACATCTTTCAGTATTGACCTAGAGACAAAGATGGTAACTGTTAGGGGACACGTTTCAGCAGTTGGAGTTGTGGAGAGCATTTCAAAAGTGAAGAAGGCAGAGTTATTAGCCTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HMA

Protein Analysis

255

Amino Acids

27.65

Weight (kDa)

9.99

Isoelectric Point (pI)

61.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HMA PF00403 193 - 247 1.1e-11 Heavy-metal-associated domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014749)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g30890
malus_domestica MD08G1221300.v1.1 MD15G1412000.v1.1
prunus_persica Prupe.1G558400_v2.0.a1 Prupe.1G558400_v2.0.a1
pyrus_communis pycom08g19190 pycom15g36680
rosa_chinensis RchiOBHm_Chr7g0226171
rosa_laevigata RLG00000001761
rosa_multiflora Rmu_sc0001350.1_g000012
rosa_roxburghii Rroxscaffold_3G00233160
rosa_rugosa Rorug07G0235200
rosa_samantha Rh7AG382200 Rh7BG369200 Rh7CG401700 Rh7DG380600
rosa_wichuraiana Rw7G032000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 419
AccB7I CCANNNNNTGG 2 cut(s) 404, 571
AccII CGCG 1 cut(s) 551
AciI CCGC 9 cut(s) 124, 129, 200, 236, 268, 348, 536, 549, 551
AclWI GGATC 1 cut(s) 515
AcoI YGGCCR 1 cut(s) 406
AcuI CTGAAG 1 cut(s) 638
AcyI GRCGYC 1 cut(s) 416
AfaI GTAC 3 cut(s) 19, 133, 325
AfiI CCNNNNNNNGG 3 cut(s) 187, 404, 571
AflIII ACRYGT 1 cut(s) 697
AgsI TTSAA 1 cut(s) 732
AhlI ACTAGT 1 cut(s) 320
AjnI CCWGG 2 cut(s) 371, 564
AluBI AGCT 3 cut(s) 251, 257, 401
AluI AGCT 3 cut(s) 251, 257, 401
Alw26I GTCTC 3 cut(s) 189, 407, 665
AlwI GGATC 1 cut(s) 515
AoxI GGCC 1 cut(s) 406
ApeKI GCWGC 6 cut(s) 126, 251, 254, 345, 456, 481
AsuHPI GGTGA 2 cut(s) 16, 350
BalI TGGCCA 1 cut(s) 408
BbvI GCAGC 6 cut(s) 138, 238, 266, 357, 468, 493
BccI CCATC 4 cut(s) 335, 520, 631, 673
BciT130I CCWGG 2 cut(s) 373, 566
BclI TGATCA 1 cut(s) 475
BcoDI GTCTC 3 cut(s) 189, 407, 665
BcuI ACTAGT 1 cut(s) 320
BfaI CTAG 5 cut(s) 15, 21, 321, 453, 668
BfmI CTRYAG 1 cut(s) 252
BisI GCNGC 8 cut(s) 124, 127, 236, 252, 255, 346, 457, 482
BlsI GCNGC 8 cut(s) 125, 128, 237, 253, 256, 347, 458, 483
BmcAI AGTACT 2 cut(s) 19, 325
Bme1390I CCNGG 2 cut(s) 373, 566
BmiI GGNNCC 1 cut(s) 377
BmrFI CCNGG 2 cut(s) 373, 566
BmsI GCATC 1 cut(s) 205
BpmI CTGGAG 2 cut(s) 355, 381
BsaHI GRCGYC 1 cut(s) 416
BsaJI CCNNGG 2 cut(s) 549, 598
BsaXI ACNNNNNCTCC 4 cut(s) 353, 376, 383, 406
Bsc4I CCNNNNNNNGG 3 cut(s) 187, 404, 571
Bse1I ACTGG 2 cut(s) 50, 181
Bse3DI GCAATG 1 cut(s) 592
BseBI CCWGG 2 cut(s) 373, 566
BseDI CCNNGG 2 cut(s) 549, 598
BseGI GGATG 2 cut(s) 545, 608
BseLI CCNNNNNNNGG 3 cut(s) 187, 404, 571
BseMI GCAATG 1 cut(s) 592
BseMII CTCAG 1 cut(s) 200
BseNI ACTGG 2 cut(s) 50, 181
BseRI GAGGAG 4 cut(s) 368, 378, 381, 384
BseXI GCAGC 6 cut(s) 138, 238, 266, 357, 468, 493
Bsh1236I CGCG 1 cut(s) 551
Bsh1285I CGRYCG 1 cut(s) 501
BshFI GGCC 1 cut(s) 408
BsiEI CGRYCG 1 cut(s) 501
BslFI GGGAC 1 cut(s) 708
BslI CCNNNNNNNGG 3 cut(s) 187, 404, 571
BsmAI GTCTC 3 cut(s) 189, 407, 665
BsmBI CGTCTC 1 cut(s) 407
BsmFI GGGAC 1 cut(s) 708
BsmI GAATGC 1 cut(s) 459
BsnI GGCC 1 cut(s) 408
Bsp143I GATC 4 cut(s) 314, 475, 498, 520
BspACI CCGC 9 cut(s) 124, 129, 200, 236, 268, 348, 536, 549, 551
BspANI GGCC 1 cut(s) 408
BspCNI CTCAG 1 cut(s) 199
BspFNI CGCG 1 cut(s) 551
BspHI TCATGA 1 cut(s) 108
BspLI GGNNCC 1 cut(s) 377
BspMAI CTGCAG 1 cut(s) 256
BspPI GGATC 1 cut(s) 515
BsrDI GCAATG 1 cut(s) 592
BsrI ACTGG 2 cut(s) 50, 181
BssECI CCNNGG 2 cut(s) 549, 598
BssMI GATC 4 cut(s) 314, 475, 498, 520
BssNI GRCGYC 1 cut(s) 416
BssT1I CCWWGG 1 cut(s) 598
Bst2UI CCWGG 2 cut(s) 373, 566
Bst4CI ACNGT 4 cut(s) 38, 265, 431, 688
BstACI GRCGYC 1 cut(s) 416
BstC8I GCNNGC 2 cut(s) 220, 437
BstDEI CTNAG 3 cut(s) 135, 186, 633
BstDSI CCRYGG 1 cut(s) 549
BstF5I GGATG 2 cut(s) 545, 608
BstFNI CGCG 1 cut(s) 551
BstKTI GATC 4 cut(s) 317, 478, 501, 523
BstMAI GTCTC 3 cut(s) 189, 407, 665
BstMBI GATC 4 cut(s) 314, 475, 498, 520
BstMCI CGRYCG 1 cut(s) 501
BstMWI GCNNNNNNNGC 1 cut(s) 441
BstNI CCWGG 2 cut(s) 373, 566
BstSCI CCNGG 2 cut(s) 371, 564
BstSFI CTRYAG 1 cut(s) 252
BstUI CGCG 1 cut(s) 551
BstV1I GCAGC 6 cut(s) 138, 238, 266, 357, 468, 493
BsuRI GGCC 1 cut(s) 408
BtgI CCRYGG 1 cut(s) 549
BtsCI GGATG 2 cut(s) 545, 608
BtsIMutI CAGTG 1 cut(s) 261
Cac8I GCNNGC 2 cut(s) 220, 437
CciI TCATGA 1 cut(s) 108
Cfr42I CCGCGG 1 cut(s) 552
Csp6I GTAC 3 cut(s) 18, 132, 324
CviAII CATG 8 cut(s) 109, 115, 175, 290, 301, 404, 410, 473
CviQI GTAC 3 cut(s) 18, 132, 324
DdeI CTNAG 3 cut(s) 135, 186, 633
DpnI GATC 4 cut(s) 316, 477, 500, 522
DpnII GATC 4 cut(s) 314, 475, 498, 520
EaeI YGGCCR 1 cut(s) 406
EciI GGCGGA 1 cut(s) 257
Eco130I CCWWGG 1 cut(s) 598
Eco57I CTGAAG 1 cut(s) 638
EcoRII CCWGG 2 cut(s) 371, 564
EcoT14I CCWWGG 1 cut(s) 598
ErhI CCWWGG 1 cut(s) 598
Esp3I CGTCTC 1 cut(s) 407
FaeI CATG 8 cut(s) 112, 118, 178, 293, 304, 407, 413, 476
FaqI GGGAC 1 cut(s) 708
FatI CATG 8 cut(s) 108, 114, 174, 289, 300, 403, 409, 472
FbaI TGATCA 1 cut(s) 475
Fnu4HI GCNGC 8 cut(s) 124, 127, 236, 252, 255, 346, 457, 482
FokI GGATG 2 cut(s) 532, 615
Fsp4HI GCNGC 8 cut(s) 124, 127, 236, 252, 255, 346, 457, 482
FspBI CTAG 5 cut(s) 15, 21, 321, 453, 668
GluI GCNGC 8 cut(s) 124, 127, 236, 252, 255, 346, 457, 482
GsuI CTGGAG 2 cut(s) 355, 381
HaeIII GGCC 1 cut(s) 408
Hin1I GRCGYC 1 cut(s) 416
Hin1II CATG 8 cut(s) 112, 118, 178, 293, 304, 407, 413, 476
HphI GGTGA 2 cut(s) 16, 350
Hpy166II GTNNAC 1 cut(s) 614
Hpy188I TCNGA 3 cut(s) 214, 503, 520
Hpy188III TCNNGA 3 cut(s) 109, 524, 762
Hpy8I GTNNAC 1 cut(s) 614
HpyAV CCTTC 3 cut(s) 4, 635, 736
HpyCH4III ACNGT 4 cut(s) 38, 265, 431, 688
HpyCH4IV ACGT 2 cut(s) 416, 699
HpyCH4V TGCA 1 cut(s) 254
HpyF10VI GCNNNNNNNGC 1 cut(s) 441
HpyF3I CTNAG 3 cut(s) 135, 186, 633
HpySE526I ACGT 2 cut(s) 416, 699
Hsp92I GRCGYC 1 cut(s) 416
Hsp92II CATG 8 cut(s) 112, 118, 178, 293, 304, 407, 413, 476
Ksp22I TGATCA 1 cut(s) 475
KspI CCGCGG 1 cut(s) 552
Kzo9I GATC 4 cut(s) 314, 475, 498, 520
LmnI GCTCC 2 cut(s) 227, 381
Lsp1109I GCAGC 6 cut(s) 138, 238, 266, 357, 468, 493
LweI GCATC 1 cut(s) 205
MaeI CTAG 5 cut(s) 15, 21, 321, 453, 668
MaeII ACGT 2 cut(s) 416, 699
MaeIII GTNAC 2 cut(s) 646, 682
MalI GATC 4 cut(s) 316, 477, 500, 522
MboI GATC 4 cut(s) 314, 475, 498, 520
MboII GAAGA 7 cut(s) 19, 160, 163, 166, 324, 631, 751
MlsI TGGCCA 1 cut(s) 408
MluCI AATT 3 cut(s) 30, 87, 98
MluNI TGGCCA 1 cut(s) 408
MmeI TCCRAC 1 cut(s) 691
Mox20I TGGCCA 1 cut(s) 408
MscI TGGCCA 1 cut(s) 408
MseI TTAA 2 cut(s) 33, 465
MslI CAYNNNNRTG 3 cut(s) 113, 305, 408
Msp20I TGGCCA 1 cut(s) 408
MspA1I CMGCKG 4 cut(s) 129, 251, 348, 551
MspR9I CCNGG 2 cut(s) 373, 566
Mva1269I GAATGC 1 cut(s) 459
MvaI CCWGG 2 cut(s) 373, 566
MvnI CGCG 1 cut(s) 551
MwoI GCNNNNNNNGC 1 cut(s) 441
NdeII GATC 4 cut(s) 314, 475, 498, 520
NlaIII CATG 8 cut(s) 112, 118, 178, 293, 304, 407, 413, 476
NlaIV GGNNCC 1 cut(s) 377
PagI TCATGA 1 cut(s) 108
PctI GAATGC 1 cut(s) 459
PflMI CCANNNNNTGG 2 cut(s) 404, 571
PkrI GCNGC 8 cut(s) 125, 128, 237, 253, 256, 347, 458, 483
Ple19I CGATCG 1 cut(s) 501
Psp6I CCWGG 2 cut(s) 371, 564
PspGI CCWGG 2 cut(s) 371, 564
PspN4I GGNNCC 1 cut(s) 377
PstI CTGCAG 1 cut(s) 256
PvuI CGATCG 1 cut(s) 501
PvuII CAGCTG 1 cut(s) 251
RsaI GTAC 3 cut(s) 19, 133, 325
RsaNI GTAC 3 cut(s) 18, 132, 324
RseI CAYNNNNRTG 3 cut(s) 113, 305, 408
SacII CCGCGG 1 cut(s) 552
SaqAI TTAA 2 cut(s) 33, 465
SatI GCNGC 8 cut(s) 124, 127, 236, 252, 255, 346, 457, 482
Sau3AI GATC 4 cut(s) 314, 475, 498, 520
ScaI AGTACT 2 cut(s) 19, 325
ScrFI CCNGG 2 cut(s) 373, 566
SetI ASST 9 cut(s) 192, 253, 259, 403, 419, 471, 570, 669, 702
SfaNI GCATC 1 cut(s) 205
SfcI CTRYAG 1 cut(s) 252
Sfr303I CCGCGG 1 cut(s) 552
SgrBI CCGCGG 1 cut(s) 552
SmiMI CAYNNNNRTG 3 cut(s) 113, 305, 408
SpeI ACTAGT 1 cut(s) 320
Sse9I AATT 3 cut(s) 30, 87, 98
SsiI CCGC 9 cut(s) 124, 129, 200, 236, 268, 348, 536, 549, 551
SspMI CTAG 5 cut(s) 15, 21, 321, 453, 668
StyD4I CCNGG 2 cut(s) 371, 564
StyI CCWWGG 1 cut(s) 598
TaaI ACNGT 4 cut(s) 38, 265, 431, 688
TaiI ACGT 2 cut(s) 419, 702
TaqI TCGA 1 cut(s) 497
TasI AATT 3 cut(s) 30, 87, 98
TatI WGTACW 2 cut(s) 17, 323
TauI GCSGC 2 cut(s) 126, 238
Tru1I TTAA 2 cut(s) 33, 465
Tru9I TTAA 2 cut(s) 33, 465
TscAI CASTG 1 cut(s) 268
TseI GCWGC 6 cut(s) 126, 251, 254, 345, 456, 481
TspDTI ATGAA 5 cut(s) 125, 131, 161, 163, 581
TspGWI ACGGA 1 cut(s) 214
TspRI CASTG 1 cut(s) 268
Van91I CCANNNNNTGG 2 cut(s) 404, 571
XcmI CCANNNNNNNNNTGG 1 cut(s) 605
XspI CTAG 5 cut(s) 15, 21, 321, 453, 668
ZraI GACGTC 1 cut(s) 417
ZrmI AGTACT 2 cut(s) 19, 325
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.