pycom16g00370

Photosystem II stability assembly factor HCF136

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0009356
Physical Location & Seq
Reverse (-)
209221 .. 211642
2422 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1221 bp
ATGGCGCCTACTCTGCAACTGACGACGGACTTCTCCAAACCAACAATTCACCTGAAACCCTCTCTCGAATTCGCAACCACTCCTCGCCATTTCCGGACCTCTCTATCTATACCTCGAGCTTCTTCTTCGTCCTCCGATTCTTCCCTCGTCAGTCGCAGACTCTTCGTTTCTGAGACGGCGGCGCTGTCTCTATCCCTCACCACTTTGCCGCTGTTCGGATCCATACTTCCGGCCAAGGCGGAGGATCCCGCGCTTTCGGAGTGGGAGAAAGTTTCCCTTCCTATCGACCCGGGGGTCGTCCTCCTCGACATTGCTTTTGTACCCGAGGACCCCAAGCATGGTTTTCTTTTGGGGACCAGGCAAACCCTTTTAGAGACGAAGGATGGCGGAGAAACTTGGGTTCCACGTTCGATTCCGTCAGCGGAAGATGAAGATTTTAATTACAGATTCAATGCCATAAGCTTCAAAGGGAAGGAGGGTTGGATTGTTGGAAAGCCTGCAATTCTTTTGCACACTTCTGATTCCGGAGACAGCTGGGAACGGATACCGTTAAGCTCTCAGCTTCCTGGAGATATGGTGTATATAAAGGCAACTGGTGAGAAGAGTGCAGAGATGGTGACTGACGAAGGTGCAATTTATGTGACATCAAATAGAGGCTATAACTGGAGGGCTGCTGTTCAGGAGACTGTTTCCGCTACCCTTAATAGAACAGTGTCTAGTGGAATTAGTGGTGCAAGTTACTACACCGGAACTTTTAATTCTGTGAATCGCTCACCCGATGGAAAATATGTTGCTGTCTCAAGCCGTGGTAACTTCTATCTCACTTGGGAGCCTGGCCAGCCATACTGGCAGCCACATAACAGAACAGTTGCAAGAAGAATTCAAAACATGGGATGGAGAGCTGATGGTGGTCTTTGGCTCCTTGTTCGTGGAGGGGGACTTTATCTGAGCAAAGGTACCGGGTTAACTGAGGATTTTGAAGAAGTTCCAGTCCAAAGCCGGGGTTTTGGCATTCTTGATGTCGGGTACCGATCAAAGGATGAGGCTTGGGCAGCTGGGGGAAGTGGGATCTTACTGCAAACTACCAATGGTGGCAAGACATGGACACGTGACAAAGCAGCGGACAATATTGCAGCGAATCTATACTCTGTAAAGTTTATTGATGACAAAAAAGGATTTGTGCTGGGGAATGACGGGGTCTTGCTTCGGTATCTTGGATAA

Protein Analysis

407

Amino Acids

44.32

Weight (kDa)

6.27

Isoelectric Point (pI)

33.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PSII_BNR PF14870 84 - 404 5.9e-125 Photosynthesis system II assembly factor YCF48
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 293
Acc65I GGTACC 2 cut(s) 956, 1026
AccB1I GGYRCC 3 cut(s) 4, 956, 1026
AccII CGCG 1 cut(s) 251
AccIII TCCGGA 2 cut(s) 93, 524
AciI CCGC 8 cut(s) 179, 209, 239, 249, 387, 422, 693, 1121
AclWI GGATC 5 cut(s) 213, 226, 239, 252, 1076
AcoI YGGCCR 2 cut(s) 231, 835
AcsI RAATTY 2 cut(s) 68, 879
AcvI CACGTG 1 cut(s) 1109
AcyI GRCGYC 1 cut(s) 5
AfaI GTAC 3 cut(s) 321, 958, 1028
AfiI CCNNNNNNNGG 4 cut(s) 215, 338, 1000, 1036
AflIII ACRYGT 1 cut(s) 1106
AgsI TTSAA 4 cut(s) 451, 466, 884, 980
AjnI CCWGG 3 cut(s) 356, 565, 832
AluBI AGCT 7 cut(s) 119, 462, 534, 555, 562, 902, 1055
AluI AGCT 7 cut(s) 119, 462, 534, 555, 562, 902, 1055
Alw26I GTCTC 6 cut(s) 167, 192, 368, 522, 677, 802
AlwI GGATC 5 cut(s) 213, 226, 239, 252, 1076
Ama87I CYCGRG 3 cut(s) 114, 289, 323
Aor13HI TCCGGA 2 cut(s) 93, 524
AoxI GGCC 2 cut(s) 231, 835
ApeKI GCWGC 5 cut(s) 671, 850, 1052, 1118, 1133
ApoI RAATTY 2 cut(s) 68, 879
ArsI GACNNNNNNTTYG 2 cut(s) 299, 331
Asp700I GAANNNNTTC 1 cut(s) 984
Asp718I GGTACC 2 cut(s) 956, 1026
AspLEI GCGC 3 cut(s) 7, 184, 253
AspS9I GGNCC 3 cut(s) 96, 328, 354
AsuC2I CCSGG 4 cut(s) 290, 291, 961, 1001
AsuHPI GGTGA 5 cut(s) 41, 190, 608, 628, 765
AvaI CYCGRG 3 cut(s) 114, 289, 323
AvaII GGWCC 3 cut(s) 96, 328, 354
BalI TGGCCA 1 cut(s) 837
BamHI GGATCC 2 cut(s) 218, 244
BanI GGYRCC 3 cut(s) 4, 956, 1026
BbrPI CACGTG 1 cut(s) 1109
BbvI GCAGC 5 cut(s) 658, 862, 1064, 1130, 1145
BccI CCATC 5 cut(s) 377, 607, 773, 888, 899
BceAI ACGGC 2 cut(s) 192, 789
BcgI CGANNNNNNTGC 2 cut(s) 145, 179
BciT130I CCWGG 3 cut(s) 358, 567, 834
BciVI GTATCC 1 cut(s) 537
BcnI CCSGG 4 cut(s) 290, 291, 961, 1001
BcoDI GTCTC 6 cut(s) 167, 192, 368, 522, 677, 802
BfaI CTAG 1 cut(s) 717
BfoI RGCGCY 2 cut(s) 8, 185
BfuI GTATCC 1 cut(s) 537
BglI GCCNNNNNGGC 1 cut(s) 847
BisI GCNGC 7 cut(s) 180, 209, 672, 851, 1053, 1119, 1134
BlsI GCNGC 7 cut(s) 181, 210, 673, 852, 1054, 1120, 1135
Bme1390I CCNGG 7 cut(s) 290, 291, 358, 567, 834, 961, 1001
Bme18I GGWCC 3 cut(s) 96, 328, 354
BmeT110I CYCGRG 3 cut(s) 114, 289, 323
BmgT120I GGNCC 3 cut(s) 96, 328, 354
BmrFI CCNGG 7 cut(s) 290, 291, 358, 567, 834, 961, 1001
BpmI CTGGAG 2 cut(s) 588, 685
BpuEI CTTGAG 1 cut(s) 784
BpuMI CCSGG 4 cut(s) 290, 291, 961, 1001
BsaAI YACGTR 1 cut(s) 1109
BsaHI GRCGYC 1 cut(s) 5
BsaJI CCNNGG 6 cut(s) 234, 289, 290, 324, 805, 1000
BsaWI WCCGGW 3 cut(s) 93, 524, 746
BsaXI ACNNNNNCTCC 2 cut(s) 561, 591
Bsc4I CCNNNNNNNGG 4 cut(s) 215, 338, 1000, 1036
Bse1I ACTGG 4 cut(s) 598, 668, 851, 989
Bse3DI GCAATG 1 cut(s) 309
BseAI TCCGGA 2 cut(s) 93, 524
BseBI CCWGG 3 cut(s) 358, 567, 834
BseDI CCNNGG 6 cut(s) 234, 289, 290, 324, 805, 1000
BseGI GGATG 3 cut(s) 388, 899, 1045
BseLI CCNNNNNNNGG 4 cut(s) 215, 338, 1000, 1036
BseMI GCAATG 1 cut(s) 309
BseMII CTCAG 4 cut(s) 162, 572, 938, 960
BseNI ACTGG 4 cut(s) 598, 668, 851, 989
BseRI GAGGAG 2 cut(s) 72, 293
BseXI GCAGC 5 cut(s) 658, 862, 1064, 1130, 1145
BseYI CCCAGC 3 cut(s) 534, 1055, 1183
BsgI GTGCAG 1 cut(s) 627
Bsh1236I CGCG 1 cut(s) 251
BshFI GGCC 2 cut(s) 233, 837
BshNI GGYRCC 3 cut(s) 4, 956, 1026
BsiHKCI CYCGRG 3 cut(s) 114, 289, 323
BsiSI CCGG 7 cut(s) 94, 230, 290, 525, 747, 960, 1000
BslFI GGGAC 2 cut(s) 367, 951
BslI CCNNNNNNNGG 4 cut(s) 215, 338, 1000, 1036
BsmAI GTCTC 6 cut(s) 167, 192, 368, 522, 677, 802
BsmBI CGTCTC 2 cut(s) 167, 368
BsmFI GGGAC 2 cut(s) 367, 951
BsmI GAATGC 1 cut(s) 1011
BsnI GGCC 2 cut(s) 233, 837
BsoBI CYCGRG 3 cut(s) 114, 289, 323
Bsp13I TCCGGA 2 cut(s) 93, 524
Bsp143I GATC 4 cut(s) 218, 244, 1031, 1068
BspACI CCGC 8 cut(s) 179, 209, 239, 249, 387, 422, 693, 1121
BspANI GGCC 2 cut(s) 233, 837
BspCNI CTCAG 4 cut(s) 163, 571, 939, 961
BspEI TCCGGA 2 cut(s) 93, 524
BspFNI CGCG 1 cut(s) 251
BspPI GGATC 5 cut(s) 213, 226, 239, 252, 1076
BspT107I GGYRCC 3 cut(s) 4, 956, 1026
BsrDI GCAATG 1 cut(s) 309
BsrI ACTGG 4 cut(s) 598, 668, 851, 989
BssECI CCNNGG 6 cut(s) 234, 289, 290, 324, 805, 1000
BssMI GATC 4 cut(s) 218, 244, 1031, 1068
BssNI GRCGYC 1 cut(s) 5
BssT1I CCWWGG 1 cut(s) 234
Bst2UI CCWGG 3 cut(s) 358, 567, 834
Bst4CI ACNGT 4 cut(s) 549, 688, 712, 868
Bst6I CTCTTC 2 cut(s) 167, 596
BstACI GRCGYC 1 cut(s) 5
BstBAI YACGTR 1 cut(s) 1109
BstC8I GCNNGC 2 cut(s) 498, 839
BstDEI CTNAG 4 cut(s) 171, 558, 947, 969
BstDSI CCRYGG 1 cut(s) 805
BstF5I GGATG 3 cut(s) 388, 899, 1045
BstFNI CGCG 1 cut(s) 251
BstH2I RGCGCY 2 cut(s) 8, 185
BstHHI GCGC 3 cut(s) 7, 184, 253
BstKTI GATC 4 cut(s) 221, 247, 1034, 1071
BstMAI GTCTC 6 cut(s) 167, 192, 368, 522, 677, 802
BstMBI GATC 4 cut(s) 218, 244, 1031, 1068
BstMWI GCNNNNNNNGC 4 cut(s) 13, 838, 847, 1052
BstNI CCWGG 3 cut(s) 358, 567, 834
BstSCI CCNGG 7 cut(s) 288, 289, 356, 565, 832, 959, 999
BstUI CGCG 1 cut(s) 251
BstV1I GCAGC 5 cut(s) 658, 862, 1064, 1130, 1145
BstX2I RGATCY 3 cut(s) 218, 244, 1068
BstYI RGATCY 3 cut(s) 218, 244, 1068
BsuI GTATCC 1 cut(s) 537
BsuRI GGCC 2 cut(s) 233, 837
BtgI CCRYGG 1 cut(s) 805
BtsCI GGATG 3 cut(s) 388, 899, 1045
BtsIMutI CAGTG 1 cut(s) 717
Cac8I GCNNGC 2 cut(s) 498, 839
CfoI GCGC 3 cut(s) 7, 184, 253
Cfr13I GGNCC 3 cut(s) 96, 328, 354
Cfr9I CCCGGG 1 cut(s) 289
Csp6I GTAC 3 cut(s) 320, 957, 1027
CviAII CATG 3 cut(s) 338, 889, 1101
CviQI GTAC 3 cut(s) 320, 957, 1027
DdeI CTNAG 4 cut(s) 171, 558, 947, 969
DinI GGCGCC 1 cut(s) 6
DpnI GATC 4 cut(s) 220, 246, 1033, 1070
DpnII GATC 4 cut(s) 218, 244, 1031, 1068
DrdI GACNNNNNNGTC 1 cut(s) 293
DseDI GACNNNNNNGTC 1 cut(s) 293
EaeI YGGCCR 2 cut(s) 231, 835
Eam1104I CTCTTC 2 cut(s) 167, 596
EarI CTCTTC 2 cut(s) 167, 596
EciI GGCGGA 2 cut(s) 254, 402
Eco130I CCWWGG 1 cut(s) 234
Eco47I GGWCC 3 cut(s) 96, 328, 354
Eco72I CACGTG 1 cut(s) 1109
Eco88I CYCGRG 3 cut(s) 114, 289, 323
EcoO109I RGGNCCY 1 cut(s) 328
EcoRI GAATTC 2 cut(s) 68, 879
EcoRII CCWGG 3 cut(s) 356, 565, 832
EcoT14I CCWWGG 1 cut(s) 234
EgeI GGCGCC 1 cut(s) 6
EheI GGCGCC 1 cut(s) 6
ErhI CCWWGG 1 cut(s) 234
Esp3I CGTCTC 2 cut(s) 167, 368
FaeI CATG 3 cut(s) 341, 892, 1104
FalI AAGNNNNNCTT 2 cut(s) 261, 293
FaqI GGGAC 2 cut(s) 367, 951
FatI CATG 3 cut(s) 337, 888, 1100
FauI CCCGC 1 cut(s) 256
Fnu4HI GCNGC 7 cut(s) 180, 209, 672, 851, 1053, 1119, 1134
FokI GGATG 3 cut(s) 395, 906, 1052
Fsp4HI GCNGC 7 cut(s) 180, 209, 672, 851, 1053, 1119, 1134
FspBI CTAG 1 cut(s) 717
GlaI GCGC 3 cut(s) 6, 183, 252
GluI GCNGC 7 cut(s) 180, 209, 672, 851, 1053, 1119, 1134
GsaI CCCAGC 3 cut(s) 538, 1059, 1187
GsuI CTGGAG 2 cut(s) 588, 685
HaeII RGCGCY 2 cut(s) 8, 185
HaeIII GGCC 2 cut(s) 233, 837
HapII CCGG 7 cut(s) 94, 230, 290, 525, 747, 960, 1000
HhaI GCGC 3 cut(s) 7, 184, 253
Hin1I GRCGYC 1 cut(s) 5
Hin1II CATG 3 cut(s) 341, 892, 1104
Hin6I GCGC 3 cut(s) 5, 182, 251
HinP1I GCGC 3 cut(s) 5, 182, 251
HincII GTYRAC 1 cut(s) 966
HindII GTYRAC 1 cut(s) 966
HindIII AAGCTT 1 cut(s) 460
HinfI GANTC 7 cut(s) 137, 159, 412, 447, 521, 766, 1138
HpaI GTTAAC 1 cut(s) 966
HpaII CCGG 7 cut(s) 94, 230, 290, 525, 747, 960, 1000
HphI GGTGA 5 cut(s) 41, 190, 608, 628, 765
Hpy166II GTNNAC 1 cut(s) 966
Hpy188I TCNGA 6 cut(s) 136, 172, 218, 259, 520, 948
Hpy188III TCNNGA 5 cut(s) 65, 94, 525, 680, 1016
Hpy8I GTNNAC 1 cut(s) 966
Hpy99I CGWCG 1 cut(s) 28
HpyAV CCTTC 4 cut(s) 287, 373, 466, 620
HpyCH4III ACNGT 4 cut(s) 549, 688, 712, 868
HpyCH4IV ACGT 2 cut(s) 406, 1108
HpyCH4V TGCA 9 cut(s) 16, 500, 511, 608, 632, 734, 872, 1078, 1133
HpyF10VI GCNNNNNNNGC 4 cut(s) 13, 838, 847, 1052
HpyF3I CTNAG 4 cut(s) 171, 558, 947, 969
HpySE526I ACGT 2 cut(s) 406, 1108
Hsp92I GRCGYC 1 cut(s) 5
Hsp92II CATG 3 cut(s) 341, 892, 1104
HspAI GCGC 3 cut(s) 5, 182, 251
KasI GGCGCC 1 cut(s) 4
Kpn2I TCCGGA 2 cut(s) 93, 524
KpnI GGTACC 2 cut(s) 960, 1030
KspAI GTTAAC 1 cut(s) 966
Kzo9I GATC 4 cut(s) 218, 244, 1031, 1068
LmnI GCTCC 2 cut(s) 829, 924
Lsp1109I GCAGC 5 cut(s) 658, 862, 1064, 1130, 1145
MaeI CTAG 1 cut(s) 717
MaeII ACGT 2 cut(s) 406, 1108
MaeIII GTNAC 5 cut(s) 616, 640, 737, 809, 1109
MalI GATC 4 cut(s) 220, 246, 1033, 1070
MboI GATC 4 cut(s) 218, 244, 1031, 1068
MboII GAAGA 9 cut(s) 114, 117, 132, 154, 437, 443, 613, 888, 992
MflI RGATCY 3 cut(s) 218, 244, 1068
MlsI TGGCCA 1 cut(s) 837
MluCI AATT 8 cut(s) 45, 68, 439, 501, 633, 723, 757, 879
MluNI TGGCCA 1 cut(s) 837
Mly113I GGCGCC 1 cut(s) 5
MlyI GAGTC 1 cut(s) 153
MmeI TCCRAC 2 cut(s) 461, 469
Mox20I TGGCCA 1 cut(s) 837
MroI TCCGGA 2 cut(s) 93, 524
MroXI GAANNNNTTC 1 cut(s) 984
MscI TGGCCA 1 cut(s) 837
MseI TTAA 5 cut(s) 438, 551, 702, 756, 965
Msp20I TGGCCA 1 cut(s) 837
MspA1I CMGCKG 5 cut(s) 211, 422, 534, 1055, 1121
MspI CCGG 7 cut(s) 94, 230, 290, 525, 747, 960, 1000
MspR9I CCNGG 7 cut(s) 290, 291, 358, 567, 834, 961, 1001
Mva1269I GAATGC 1 cut(s) 1011
MvaI CCWGG 3 cut(s) 358, 567, 834
MvnI CGCG 1 cut(s) 251
MwoI GCNNNNNNNGC 4 cut(s) 13, 838, 847, 1052
NarI GGCGCC 1 cut(s) 5
NciI CCSGG 4 cut(s) 290, 291, 961, 1001
NdeII GATC 4 cut(s) 218, 244, 1031, 1068
NlaIII CATG 3 cut(s) 341, 892, 1104
NmuCI GTSAC 3 cut(s) 616, 640, 1109
PaeR7I CTCGAG 1 cut(s) 114
PcsI WCGNNNNNNNCGW 1 cut(s) 303
PctI GAATGC 1 cut(s) 1011
PdmI GAANNNNTTC 1 cut(s) 984
PfeI GAWTC 6 cut(s) 137, 412, 447, 521, 766, 1138
PflFI GACNNNGTC 1 cut(s) 1196
PfoI TCCNGGA 1 cut(s) 565
PkrI GCNGC 7 cut(s) 181, 210, 673, 852, 1054, 1120, 1135
PleI GAGTC 1 cut(s) 153
PluTI GGCGCC 1 cut(s) 8
PmaCI CACGTG 1 cut(s) 1109
PmlI CACGTG 1 cut(s) 1109
PpsI GAGTC 1 cut(s) 153
Ppu21I YACGTR 1 cut(s) 1109
PpuMI RGGWCCY 1 cut(s) 328
Psp5II RGGWCCY 1 cut(s) 328
Psp6I CCWGG 3 cut(s) 356, 565, 832
PspCI CACGTG 1 cut(s) 1109
PspFI CCCAGC 3 cut(s) 534, 1055, 1183
PspGI CCWGG 3 cut(s) 356, 565, 832
PspPI GGNCC 3 cut(s) 96, 328, 354
PspPPI RGGWCCY 1 cut(s) 328
PspXI VCTCGAGB 1 cut(s) 114
PsuI RGATCY 3 cut(s) 218, 244, 1068
PsyI GACNNNGTC 1 cut(s) 1196
PvuII CAGCTG 2 cut(s) 534, 1055
RsaI GTAC 3 cut(s) 321, 958, 1028
RsaNI GTAC 3 cut(s) 320, 957, 1027
SaqAI TTAA 5 cut(s) 438, 551, 702, 756, 965
SatI GCNGC 7 cut(s) 180, 209, 672, 851, 1053, 1119, 1134
Sau3AI GATC 4 cut(s) 218, 244, 1031, 1068
Sau96I GGNCC 3 cut(s) 96, 328, 354
SchI GAGTC 1 cut(s) 153
ScrFI CCNGG 7 cut(s) 290, 291, 358, 567, 834, 961, 1001
SfoI GGCGCC 1 cut(s) 6
Sfr274I CTCGAG 1 cut(s) 114
SinI GGWCC 3 cut(s) 96, 328, 354
SlaI CTCGAG 1 cut(s) 114
SmaI CCCGGG 1 cut(s) 291
SmlI CTYRAG 2 cut(s) 114, 799
SmoI CTYRAG 2 cut(s) 114, 799
Sse9I AATT 8 cut(s) 45, 68, 439, 501, 633, 723, 757, 879
SsiI CCGC 8 cut(s) 179, 209, 239, 249, 387, 422, 693, 1121
SspDI GGCGCC 1 cut(s) 4
SspI AATATT 1 cut(s) 1129
SspMI CTAG 1 cut(s) 717
StyD4I CCNGG 7 cut(s) 288, 289, 356, 565, 832, 959, 999
StyI CCWWGG 1 cut(s) 234
TaaI ACNGT 4 cut(s) 549, 688, 712, 868
TaiI ACGT 2 cut(s) 409, 1111
TaqI TCGA 5 cut(s) 66, 115, 285, 306, 410
TasI AATT 8 cut(s) 45, 68, 439, 501, 633, 723, 757, 879
TauI GCSGC 2 cut(s) 182, 211
TfiI GAWTC 6 cut(s) 137, 412, 447, 521, 766, 1138
Tru1I TTAA 5 cut(s) 438, 551, 702, 756, 965
Tru9I TTAA 5 cut(s) 438, 551, 702, 756, 965
TscAI CASTG 1 cut(s) 717
TseFI GTSAC 3 cut(s) 616, 640, 1109
TseI GCWGC 5 cut(s) 671, 850, 1052, 1118, 1133
Tsp45I GTSAC 3 cut(s) 616, 640, 1109
TspDTI ATGAA 1 cut(s) 444
TspGWI ACGGA 3 cut(s) 41, 405, 556
TspMI CCCGGG 1 cut(s) 289
TspRI CASTG 1 cut(s) 717
Tth111I GACNNNGTC 1 cut(s) 1196
VpaK11BI GGWCC 3 cut(s) 96, 328, 354
XapI RAATTY 2 cut(s) 68, 879
XhoI CTCGAG 1 cut(s) 114
XmaI CCCGGG 1 cut(s) 289
XmnI GAANNNNTTC 1 cut(s) 984
XspI CTAG 1 cut(s) 717
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.