pycom16g00390

Belongs to the glycosyl hydrolase 9 (cellulase E) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0013278
Physical Location & Seq
Forward (+)
232975 .. 236037
3063 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1635 bp
ATGACGAGGAACTGCAACCTTACACCGTGCGTTTTGCTAGCCTTGCTACTACTCGGTGTTCTCCACCTTGAATCAGCCGCCGCGACATACTTCACCTACGGCGAGGCCCTGGACAAGACCTTGATGTTTTTCGAGGCACAAAGGTCAGGTAAGTTGCCGGAAACTCAGCGTGTCAACTGGCGCGGTGACTCGGGACTCAAGGACGGTCTTGTTCAAGGGGTGAACTTGGTAGGAGGGTATTATGATGCAGGTGATCATGTGAAATTTGGACTGCCGATGGCGTTTACAGTGACAATGCTTTCATGGGGAGCTGTTGAATACAGAAAGGAAATTTCGGATTTAGCACAACTGGGACAAACCTTACGGGCCATCCGATGGGGCACTGACTACTTCATGAAAGCACATCCACAACCTAATGTTCTCTGGGGACAGGTAGGAGATGGGGACTCGGATCACTATTGCTGGGAGCGTGCAGAAGATATGACAACTCCAAGAACTTCTTACAAGATTGACGAAAGCCATCCCGGTTCGGACCTTGCTGGAGAAACTGCAGCCGCCTTGGCATCGGCCGCCATAGCTTTCAAGCCTTACAACTCTTCCTACTCTGCTCTCCTCTTAGTTCATGCAAAGCAGGTGTTGTTGGAGGGAAGAGGCGGTGCATACACTTCCACGCTAAAACAATATCAGGCCAAAGCAGATTACTTTGCATGTGCTTGTCTCCAGAAAAACGATGGCTACAATGTCCAGAAAACTCCTGGAGGACTATTGTACGTGAGGGAATGGAACAACTTGCAGTACGTGACATCGGTAGCATTTCTCCTAGCCGTCTACTCAGATTATCTCTCCGCCGCAAACGCCAAGCTAGCTTGTCCCGACGGCCAAGCCCAGCCCCAAGAGCTCCTCAACTTCGCCAAGTCACAGGCAGACTACATACTCGGCAAAAACCCCAAGTCCATGAGCTACATGGTTGGGATTGGACAAAATTATCCAGTCCATGTCCACCACAGAGGCGCTTCAATTCCCTCTGTTTTTACTCTCCACTCTGCGGTCGAATGTGTGCAAGGTTTTGAGATGTGGTACCACCGCCCCGAGGCAAACCCCAACGTCATCTACGGTGCCCTTGTGGGAGGCCCTGACCAGAATGACAACTTCTCCGACGACCGCTCTAGTTATGAACAGACTGAGCCTACGCTTTCCGGCACCGGTCCCCTTGTTGGCCTCTTGTCTAAGTTGCAGAGTTTGAATGGCATCCCAAGTTATAAAAAAGGCTCCCCAAACTCCCAGCCAACCATACCAAGGGAAGGTGCCAATGACCATGAAGAACAAAATCCAGTACAAGCTCCAGAGACCCCAATCAAACTGATTCACTCAATCGGCAACACATGGACTGTTGGGAAAACAACATATTATCGCCACAAGGTGGTACTGAAGAACACATCGCAGAAACCGATATCGGATGTGAAGCTGGAGATTGAAGATCTCAAAGGGTCTGTGTGGGGTCTCATCCCAACACCAGAAAAGAACATCTACGAGCTTCCTCAATGGCACAAGGTCTTGCAGCCTGGCTCTGAGTGCATTTTCATTTACGTCCAAGGTGGTCCTCAGGCAAACGTTTCCATCGTAAGCTACCATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00500 ko01100 map00500 map01100
Pfam Domains
Protein Families
CBM49 Glyco_hydro_9

Protein Analysis

545

Amino Acids

59.93

Weight (kDa)

6.15

Isoelectric Point (pI)

32.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_9 PF00759 33 - 212 8.2e-79 Glycosyl hydrolase family 9
Glyco_hydro_9 PF00759 220 - 408 3.4e-54 Glycosyl hydrolase family 9
CBM49 PF09478 453 - 531 4.4e-20 Carbohydrate binding domain CBM49
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013278)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G48930
fragaria_vesca FvH4_5g29090
malus_domestica MD16G1001700.v1.1
prunus_persica Prupe.1G346900_v2.0.a1
pyrus_communis pycom16g00390
rosa_chinensis RchiOBHm_Chr7g0221721 RchiOBHm_Chr7g0221731
rosa_laevigata RLG00000002135
rosa_multiflora Rmu_co8400965.1_g000001 Rmu_sc0003311.1_g000002
rosa_roxburghii Rroxscaffold_3G00238090
rosa_rugosa Rorug07G0203000
rosa_samantha Rh7AG343900 Rh7BG334300 Rh7CG361300 Rh7DG341700
rosa_wichuraiana Rw0G003250 Rw7G029160

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1260
AarI CACCTGC 2 cut(s) 239, 622
Acc36I ACCTGC 2 cut(s) 239, 622
Acc65I GGTACC 1 cut(s) 1077
AccB1I GGYRCC 4 cut(s) 1077, 1115, 1199, 1304
AccB7I CCANNNNNTGG 2 cut(s) 375, 1420
AccBSI CCGCTC 1 cut(s) 1164
AccI GTMKAC 1 cut(s) 828
AccII CGCG 2 cut(s) 83, 183
AclI AACGTT 1 cut(s) 1611
AclWI GGATC 1 cut(s) 459
AcoI YGGCCR 2 cut(s) 567, 877
AcsI RAATTY 2 cut(s) 263, 330
AcuI CTGAAG 1 cut(s) 1448
AdeI CACNNNGTG 1 cut(s) 1420
AfaI GTAC 5 cut(s) 770, 797, 1079, 1335, 1425
AfiI CCNNNNNNNGG 7 cut(s) 375, 1045, 1090, 1214, 1296, 1301, 1420
AgeI ACCGGT 1 cut(s) 1202
AgsI TTSAA 7 cut(s) 71, 215, 317, 583, 1017, 1243, 1475
AjnI CCWGG 3 cut(s) 108, 754, 1561
Alw21I GWGCWC 1 cut(s) 900
Alw26I GTCTC 3 cut(s) 722, 1340, 1505
AlwI GGATC 1 cut(s) 459
Ama87I CYCGRG 2 cut(s) 190, 1088
AoxI GGCC 7 cut(s) 105, 366, 567, 687, 877, 1129, 1216
ApeKI GCWGC 2 cut(s) 551, 1558
ApoI RAATTY 2 cut(s) 263, 330
AsiGI ACCGGT 1 cut(s) 1202
Asp718I GGTACC 1 cut(s) 1077
AspLEI GCGC 2 cut(s) 183, 1013
AspS9I GGNCC 6 cut(s) 106, 366, 532, 1130, 1205, 1598
AsuC2I CCSGG 1 cut(s) 525
AsuHPI GGTGA 4 cut(s) 85, 197, 232, 263
AsuNHI GCTAGC 2 cut(s) 37, 862
AvaI CYCGRG 2 cut(s) 190, 1088
AvaII GGWCC 3 cut(s) 532, 1205, 1598
AxyI CCTNAGG 1 cut(s) 1602
BaeGI GKGCMC 2 cut(s) 383, 1120
BanI GGYRCC 4 cut(s) 1077, 1115, 1199, 1304
BanII GRGCYC 1 cut(s) 900
Bbv12I GWGCWC 1 cut(s) 900
BbvI GCAGC 2 cut(s) 563, 1570
BccI CCATC 7 cut(s) 271, 369, 377, 434, 528, 725, 1625
BceAI ACGGC 3 cut(s) 115, 809, 892
BciT130I CCWGG 3 cut(s) 110, 756, 1563
BclI TGATCA 1 cut(s) 253
BcnI CCSGG 1 cut(s) 525
BcoDI GTCTC 3 cut(s) 722, 1340, 1505
BfaI CTAG 4 cut(s) 38, 821, 863, 1167
BfmI CTRYAG 1 cut(s) 549
BfoI RGCGCY 1 cut(s) 1014
BfuAI ACCTGC 2 cut(s) 239, 622
BglI GCCNNNNNGGC 1 cut(s) 560
BglII AGATCT 1 cut(s) 1477
BisI GCNGC 7 cut(s) 78, 81, 552, 555, 570, 849, 1559
BlsI GCNGC 7 cut(s) 79, 82, 553, 556, 571, 850, 1560
Bme1390I CCNGG 4 cut(s) 110, 525, 756, 1563
Bme18I GGWCC 3 cut(s) 532, 1205, 1598
BmeT110I CYCGRG 2 cut(s) 190, 1088
BmgT120I GGNCC 6 cut(s) 106, 366, 532, 1130, 1205, 1598
BmiI GGNNCC 6 cut(s) 1079, 1117, 1201, 1207, 1270, 1306
BmrFI CCNGG 4 cut(s) 110, 525, 756, 1563
BmrI ACTGGG 1 cut(s) 359
BmsI GCATC 3 cut(s) 235, 572, 1257
BmtI GCTAGC 2 cut(s) 41, 866
BmuI ACTGGG 1 cut(s) 359
BpmI CTGGAG 5 cut(s) 561, 704, 777, 1326, 1487
BpuEI CTTGAG 1 cut(s) 182
BpuMI CCSGG 1 cut(s) 525
BsaAI YACGTR 2 cut(s) 772, 799
BsaI GGTCTC 2 cut(s) 1340, 1505
BsaJI CCNNGG 5 cut(s) 108, 558, 1089, 1295, 1591
BsaWI WCCGGW 1 cut(s) 1202
BsaXI ACNNNNNCTCC 2 cut(s) 1136, 1166
Bsc4I CCNNNNNNNGG 7 cut(s) 375, 1045, 1090, 1214, 1296, 1301, 1420
Bse118I RCCGGY 1 cut(s) 1202
Bse1I ACTGG 4 cut(s) 182, 354, 989, 1331
Bse21I CCTNAGG 1 cut(s) 1602
BseBI CCWGG 3 cut(s) 110, 756, 1563
BseDI CCNNGG 5 cut(s) 108, 558, 1089, 1295, 1591
BseGI GGATG 6 cut(s) 369, 403, 520, 1248, 1462, 1503
BseLI CCNNNNNNNGG 7 cut(s) 375, 1045, 1090, 1214, 1296, 1301, 1420
BseMII CTCAG 5 cut(s) 179, 846, 1173, 1560, 1616
BseNI ACTGG 4 cut(s) 182, 354, 989, 1331
BseRI GAGGAG 2 cut(s) 602, 890
BseSI GKGCMC 2 cut(s) 383, 1120
BseX3I CGGCCG 1 cut(s) 567
BseXI GCAGC 2 cut(s) 563, 1570
BseYI CCCAGC 3 cut(s) 462, 885, 1281
BsgI GTGCAG 1 cut(s) 492
Bsh1236I CGCG 2 cut(s) 83, 183
Bsh1285I CGRYCG 3 cut(s) 570, 1050, 1162
BshFI GGCC 7 cut(s) 107, 368, 569, 689, 879, 1131, 1218
BshNI GGYRCC 4 cut(s) 1077, 1115, 1199, 1304
BshTI ACCGGT 1 cut(s) 1202
BsiEI CGRYCG 3 cut(s) 570, 1050, 1162
BsiHKAI GWGCWC 1 cut(s) 900
BsiHKCI CYCGRG 2 cut(s) 190, 1088
BsiSI CCGG 4 cut(s) 158, 525, 1197, 1203
BslFI GGGAC 6 cut(s) 207, 366, 441, 458, 855, 1191
BslI CCNNNNNNNGG 7 cut(s) 375, 1045, 1090, 1214, 1296, 1301, 1420
BsmAI GTCTC 3 cut(s) 722, 1340, 1505
BsmFI GGGAC 6 cut(s) 207, 366, 441, 458, 855, 1191
BsnI GGCC 7 cut(s) 107, 368, 569, 689, 879, 1131, 1218
Bso31I GGTCTC 2 cut(s) 1340, 1505
BsoBI CYCGRG 2 cut(s) 190, 1088
Bsp1286I GDGCHC 3 cut(s) 383, 900, 1120
Bsp143I GATC 3 cut(s) 253, 451, 1477
BspANI GGCC 7 cut(s) 107, 368, 569, 689, 879, 1131, 1218
BspCNI CTCAG 5 cut(s) 178, 845, 1174, 1561, 1615
BspFNI CGCG 2 cut(s) 83, 183
BspHI TCATGA 1 cut(s) 393
BspLI GGNNCC 6 cut(s) 1079, 1117, 1201, 1207, 1270, 1306
BspMAI CTGCAG 1 cut(s) 553
BspMI ACCTGC 2 cut(s) 239, 622
BspOI GCTAGC 2 cut(s) 41, 866
BspPI GGATC 1 cut(s) 459
BspT107I GGYRCC 4 cut(s) 1077, 1115, 1199, 1304
BspTNI GGTCTC 2 cut(s) 1340, 1505
BsrBI CCGCTC 1 cut(s) 1164
BsrFI RCCGGY 1 cut(s) 1202
BsrI ACTGG 4 cut(s) 182, 354, 989, 1331
BssAI RCCGGY 1 cut(s) 1202
BssECI CCNNGG 5 cut(s) 108, 558, 1089, 1295, 1591
BssMI GATC 3 cut(s) 253, 451, 1477
BssT1I CCWWGG 3 cut(s) 558, 1295, 1591
Bst2UI CCWGG 3 cut(s) 110, 756, 1563
Bst4CI ACNGT 5 cut(s) 27, 206, 289, 1115, 1390
Bst6I CTCTTC 2 cut(s) 601, 643
BstBAI YACGTR 2 cut(s) 772, 799
BstC8I GCNNGC 3 cut(s) 39, 471, 864
BstDEI CTNAG 7 cut(s) 165, 616, 832, 1182, 1227, 1569, 1602
BstF5I GGATG 6 cut(s) 369, 403, 520, 1248, 1462, 1503
BstFNI CGCG 2 cut(s) 83, 183
BstH2I RGCGCY 1 cut(s) 1014
BstHHI GCGC 2 cut(s) 183, 1013
BstKTI GATC 3 cut(s) 256, 454, 1480
BstMAI GTCTC 3 cut(s) 722, 1340, 1505
BstMBI GATC 3 cut(s) 253, 451, 1477
BstMCI CGRYCG 3 cut(s) 570, 1050, 1162
BstMWI GCNNNNNNNGC 8 cut(s) 43, 560, 569, 575, 854, 863, 895, 1572
BstNI CCWGG 3 cut(s) 110, 756, 1563
BstNSI RCATGY 1 cut(s) 711
BstSCI CCNGG 4 cut(s) 108, 523, 754, 1561
BstSFI CTRYAG 1 cut(s) 549
BstSLI GKGCMC 2 cut(s) 383, 1120
BstUI CGCG 2 cut(s) 83, 183
BstV1I GCAGC 2 cut(s) 563, 1570
BstX2I RGATCY 1 cut(s) 1477
BstYI RGATCY 1 cut(s) 1477
BstZI CGGCCG 1 cut(s) 567
Bsu36I CCTNAGG 1 cut(s) 1602
BsuRI GGCC 7 cut(s) 107, 368, 569, 689, 879, 1131, 1218
BtgZI GCGATG 1 cut(s) 1422
BtsCI GGATG 6 cut(s) 369, 403, 520, 1248, 1462, 1503
BtsIMutI CAGTG 2 cut(s) 294, 381
BveI ACCTGC 2 cut(s) 239, 622
Cac8I GCNNGC 3 cut(s) 39, 471, 864
CciI TCATGA 1 cut(s) 393
CfoI GCGC 2 cut(s) 183, 1013
Cfr10I RCCGGY 1 cut(s) 1202
Cfr13I GGNCC 6 cut(s) 106, 366, 532, 1130, 1205, 1598
Csp6I GTAC 5 cut(s) 769, 796, 1078, 1334, 1424
CspAI ACCGGT 1 cut(s) 1202
CviQI GTAC 5 cut(s) 769, 796, 1078, 1334, 1424
DdeI CTNAG 7 cut(s) 165, 616, 832, 1182, 1227, 1569, 1602
DpnI GATC 3 cut(s) 255, 453, 1479
DpnII GATC 3 cut(s) 253, 451, 1477
DraIII CACNNNGTG 1 cut(s) 1420
EaeI YGGCCR 2 cut(s) 567, 877
EagI CGGCCG 1 cut(s) 567
Eam1104I CTCTTC 2 cut(s) 601, 643
EarI CTCTTC 2 cut(s) 601, 643
EciI GGCGGA 1 cut(s) 835
Ecl136II GAGCTC 1 cut(s) 898
EclXI CGGCCG 1 cut(s) 567
Eco130I CCWWGG 3 cut(s) 558, 1295, 1591
Eco24I GRGCYC 1 cut(s) 900
Eco31I GGTCTC 2 cut(s) 1340, 1505
Eco32I GATATC 1 cut(s) 1452
Eco47I GGWCC 3 cut(s) 532, 1205, 1598
Eco52I CGGCCG 1 cut(s) 567
Eco53kI GAGCTC 1 cut(s) 898
Eco57I CTGAAG 1 cut(s) 1448
Eco81I CCTNAGG 1 cut(s) 1602
Eco88I CYCGRG 2 cut(s) 190, 1088
EcoICRI GAGCTC 1 cut(s) 898
EcoO109I RGGNCCY 2 cut(s) 106, 1130
EcoRII CCWGG 3 cut(s) 108, 754, 1561
EcoRV GATATC 1 cut(s) 1452
EcoT14I CCWWGG 3 cut(s) 558, 1295, 1591
EcoT38I GRGCYC 1 cut(s) 900
ErhI CCWWGG 3 cut(s) 558, 1295, 1591
FalI AAGNNNNNCTT 2 cut(s) 484, 516
FaqI GGGAC 6 cut(s) 207, 366, 441, 458, 855, 1191
FbaI TGATCA 1 cut(s) 253
FblI GTMKAC 1 cut(s) 828
Fnu4HI GCNGC 7 cut(s) 78, 81, 552, 555, 570, 849, 1559
FokI GGATG 6 cut(s) 356, 390, 507, 1235, 1469, 1490
FriOI GRGCYC 1 cut(s) 900
Fsp4HI GCNGC 7 cut(s) 78, 81, 552, 555, 570, 849, 1559
FspBI CTAG 4 cut(s) 38, 821, 863, 1167
GlaI GCGC 2 cut(s) 182, 1012
GluI GCNGC 7 cut(s) 78, 81, 552, 555, 570, 849, 1559
GsaI CCCAGC 3 cut(s) 466, 889, 1285
GsuI CTGGAG 5 cut(s) 561, 704, 777, 1326, 1487
HaeII RGCGCY 1 cut(s) 1014
HaeIII GGCC 7 cut(s) 107, 368, 569, 689, 879, 1131, 1218
HapII CCGG 4 cut(s) 158, 525, 1197, 1203
HhaI GCGC 2 cut(s) 183, 1013
Hin6I GCGC 2 cut(s) 181, 1011
HinP1I GCGC 2 cut(s) 181, 1011
HincII GTYRAC 1 cut(s) 175
HindII GTYRAC 1 cut(s) 175
HinfI GANTC 5 cut(s) 71, 188, 195, 446, 1363
HpaII CCGG 4 cut(s) 158, 525, 1197, 1203
HphI GGTGA 4 cut(s) 85, 197, 232, 263
Hpy166II GTNNAC 5 cut(s) 175, 223, 285, 829, 1000
Hpy188I TCNGA 8 cut(s) 337, 374, 451, 532, 835, 1156, 1456, 1570
Hpy188III TCNNGA 6 cut(s) 192, 394, 721, 745, 872, 1343
Hpy8I GTNNAC 5 cut(s) 175, 223, 285, 829, 1000
Hpy99I CGWCG 2 cut(s) 878, 1160
HpyAV CCTTC 1 cut(s) 1295
HpyCH4III ACNGT 5 cut(s) 27, 206, 289, 1115, 1390
HpyCH4IV ACGT 5 cut(s) 771, 798, 1104, 1587, 1611
HpyF10VI GCNNNNNNNGC 8 cut(s) 43, 560, 569, 575, 854, 863, 895, 1572
HpyF3I CTNAG 7 cut(s) 165, 616, 832, 1182, 1227, 1569, 1602
HpySE526I ACGT 5 cut(s) 771, 798, 1104, 1587, 1611
HspAI GCGC 2 cut(s) 181, 1011
KpnI GGTACC 1 cut(s) 1081
Ksp22I TGATCA 1 cut(s) 253
Kzo9I GATC 3 cut(s) 253, 451, 1477
LmnI GCTCC 5 cut(s) 308, 466, 903, 1274, 1345
Lsp1109I GCAGC 2 cut(s) 563, 1570
LweI GCATC 3 cut(s) 235, 572, 1257
MaeI CTAG 4 cut(s) 38, 821, 863, 1167
MaeII ACGT 5 cut(s) 771, 798, 1104, 1587, 1611
MaeIII GTNAC 4 cut(s) 185, 289, 799, 915
MalI GATC 3 cut(s) 255, 453, 1479
MbiI CCGCTC 1 cut(s) 1164
MboI GATC 3 cut(s) 253, 451, 1477
MboII GAAGA 6 cut(s) 488, 588, 660, 1331, 1441, 1487
MflI RGATCY 1 cut(s) 1477
MhlI GDGCHC 3 cut(s) 383, 900, 1120
MluCI AATT 4 cut(s) 263, 330, 982, 1017
MlyI GAGTC 3 cut(s) 182, 189, 440
MmeI TCCRAC 2 cut(s) 621, 1179
MseI TTAA 1 cut(s) 1633
MspI CCGG 4 cut(s) 158, 525, 1197, 1203
MspR9I CCNGG 4 cut(s) 110, 525, 756, 1563
MvaI CCWGG 3 cut(s) 110, 756, 1563
MvnI CGCG 2 cut(s) 83, 183
MwoI GCNNNNNNNGC 8 cut(s) 43, 560, 569, 575, 854, 863, 895, 1572
NciI CCSGG 1 cut(s) 525
NdeII GATC 3 cut(s) 253, 451, 1477
NheI GCTAGC 2 cut(s) 37, 862
NlaIV GGNNCC 6 cut(s) 1079, 1117, 1201, 1207, 1270, 1306
NmeAIII GCCGAG 1 cut(s) 915
NmuCI GTSAC 4 cut(s) 185, 289, 799, 915
NspI RCATGY 1 cut(s) 711
PagI TCATGA 1 cut(s) 393
PaqCI CACCTGC 2 cut(s) 239, 622
PcsI WCGNNNNNNNCGW 3 cut(s) 370, 1445, 1617
PfeI GAWTC 2 cut(s) 71, 1363
PflMI CCANNNNNTGG 2 cut(s) 375, 1420
PfoI TCCNGGA 1 cut(s) 754
PinAI ACCGGT 1 cut(s) 1202
PkrI GCNGC 7 cut(s) 79, 82, 553, 556, 571, 850, 1560
PleI GAGTC 3 cut(s) 182, 189, 440
PpsI GAGTC 3 cut(s) 182, 189, 440
Ppu21I YACGTR 2 cut(s) 772, 799
PsiI TTATAA 1 cut(s) 1260
Psp124BI GAGCTC 1 cut(s) 900
Psp1406I AACGTT 1 cut(s) 1611
Psp6I CCWGG 3 cut(s) 108, 754, 1561
PspFI CCCAGC 3 cut(s) 462, 885, 1281
PspGI CCWGG 3 cut(s) 108, 754, 1561
PspN4I GGNNCC 6 cut(s) 1079, 1117, 1201, 1207, 1270, 1306
PspPI GGNCC 6 cut(s) 106, 366, 532, 1130, 1205, 1598
PstI CTGCAG 1 cut(s) 553
PsuI RGATCY 1 cut(s) 1477
RsaI GTAC 5 cut(s) 770, 797, 1079, 1335, 1425
RsaNI GTAC 5 cut(s) 769, 796, 1078, 1334, 1424
SacI GAGCTC 1 cut(s) 900
SaqAI TTAA 1 cut(s) 1633
SatI GCNGC 7 cut(s) 78, 81, 552, 555, 570, 849, 1559
Sau3AI GATC 3 cut(s) 253, 451, 1477
Sau96I GGNCC 6 cut(s) 106, 366, 532, 1130, 1205, 1598
SchI GAGTC 3 cut(s) 182, 189, 440
ScrFI CCNGG 4 cut(s) 110, 525, 756, 1563
SduI GDGCHC 3 cut(s) 383, 900, 1120
SfaNI GCATC 3 cut(s) 235, 572, 1257
SfcI CTRYAG 1 cut(s) 549
SinI GGWCC 3 cut(s) 532, 1205, 1598
SmlI CTYRAG 1 cut(s) 197
SmoI CTYRAG 1 cut(s) 197
Sse9I AATT 4 cut(s) 263, 330, 982, 1017
SspMI CTAG 4 cut(s) 38, 821, 863, 1167
SstI GAGCTC 1 cut(s) 900
StyD4I CCNGG 4 cut(s) 108, 523, 754, 1561
StyI CCWWGG 3 cut(s) 558, 1295, 1591
TaaI ACNGT 5 cut(s) 27, 206, 289, 1115, 1390
TaiI ACGT 5 cut(s) 774, 801, 1107, 1590, 1614
TaqI TCGA 2 cut(s) 132, 1050
TasI AATT 4 cut(s) 263, 330, 982, 1017
TatI WGTACW 1 cut(s) 1333
TauI GCSGC 5 cut(s) 80, 83, 557, 572, 851
TfiI GAWTC 2 cut(s) 71, 1363
Tru1I TTAA 1 cut(s) 1633
Tru9I TTAA 1 cut(s) 1633
TscAI CASTG 2 cut(s) 294, 388
TseFI GTSAC 4 cut(s) 185, 289, 799, 915
TseI GCWGC 2 cut(s) 551, 1558
Tsp45I GTSAC 4 cut(s) 185, 289, 799, 915
TspDTI ATGAA 7 cut(s) 291, 382, 410, 611, 1188, 1332, 1570
TspRI CASTG 2 cut(s) 294, 388
Van91I CCANNNNNTGG 2 cut(s) 375, 1420
VpaK11BI GGWCC 3 cut(s) 532, 1205, 1598
XapI RAATTY 2 cut(s) 263, 330
XceI RCATGY 1 cut(s) 711
XcmI CCANNNNNNNNNTGG 3 cut(s) 728, 752, 961
XmiI GTMKAC 1 cut(s) 828
XspI CTAG 4 cut(s) 38, 821, 863, 1167
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.