Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr16

Orthogroup ID

OG0010393

Physical Location & Seq

Genomic Coordinates Reverse (-)

280510 .. 288761

Gene Structure 8252 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3048 bp

ATGAGGGGTGGAATTCGTGAGAAAGGAGCTATAGCCGTCCGCGCCCTTCTCATTTCACAACCTTACCCTCGTTTCACTTCTCCTTGTACTCCCACTCATTTGCCGCCGCCACTCCTCTCTCGCGGTCGCCCCCTCGCCGCTGCGTACAGGTGCTACTCATCCTCCGCGAAGTCCTCGTCTCCCTCCGCCATGCCGGGCGCTGAATCGCAGGGACTGGAGTGGCCGGCGAAGCGCGTCAGGGACACCTTCGTCAAGTTTTTCGAGGAGAAGGCTCACGTCTTCTGGAAGTCTAGCCCCGTCGTTCCCCACAACGATCCCACTCTTTTGTTCGCCAATGCTGGTATGAACCAGTTCAAGCCCATTTTTCTGGGGACAGCAGACCCAAACACTCAGTTGAGCAAGCTGACTCGAGCATGCAACACCCAGAAATGTATAAGAGCAGGCGGGAAGCACAACGATCTCGATGATGTAGGGAAAGACACTTACCACCACACCTTCTTTGAGATGCTTGGCAATTGGTCTTTCGGGGATTATTTCAAGAAGGAAGCCATTGGATGGGCTTGGGAGCTTCTCACAAAAGTTTATAAGCTGCCAGAAGATCGAATTTATGCCACCTATTTTGGTGGTGATGAGAAGGCTGGTCTTGCTCCTGATGATGAAGCTAGAGAGTTGTGGCTTAAGTTTCTACCAGCTGGTCATGTACTACCGTTTGGCTGTAAGGATAACTTCTGGGAGATGGGAGATACTGGTCCTTGTGGTCCTTGCACTGAAATTCATTACGATAGAATCGGAAATCGTGATGCTGCATCATTAGTCAACAACGATGATCCTACCTGCATTGAAATTTGGAACCTCGTCTTTATTCAGTTCAATAGGGAAAGTGATGGTTCTCTAAAGCCGCTCCCTGCTAAGCATGTTGACACTGGGATGGGATTTGAAAGATTAACTTCCATACTTCAGAACAAGATGAGCAATTATGATACTGATGTGTTCATGCCCATCTTTGATGCTATCCAACAGGCAACAGGGGCTCCACCATATTCTGGGAAAGTTGGTGTGGATGATGTAGACAAAATTGACATGGCATACAGAGTTGTTGCAGATCATATAAGAACTCTTTCATTTTCCATTGCTGATGGGTCTCGTCCAGGCAATGACGGCCGCGAATATGTTCTGAGGCGCATTCTTCGTCGCGCTGTTCGGTATGGAAATGATGTCCTAAAAGCTAAAGAAGGATTTTTCAATGGGCTTGTGCACGTTCTGGTGACACTGATGGGTGATGTTTTCCCAGAGGTACAACAACATGAAGCACATATCAGGGAGATAATCAAAGAGGAGGAAGAAACTTTTGAGAAGACTTTACAGAAGGGAATAGAAAGATTCAAGAAGGCTGCTCAGGATGTTCAAGGGAAAACATTTAGTGGACAGGATGCTTTTGTTTTGTGGGACACATTTGGGTTTCCATTAGATTTGACTCAGTTGATGGCAGAGGAAAGGGGATTGATAGTTGATGTTGAGGGTTTCAATAATGCTATGGATGAAGCTCGAGAAAGATCAAGGAGTGCTCAAAATAAGCAAGCTGGTGGTGCTATTGTCATGGATGCTGATGCTACTGCTACATTGCACAAGAGGGGAGTTGCTGCAACAGATGACAACTTTAAATTTATTTGGAACCAGGACCATGAAAGTGTGGTAAAAGCAATTTACACTGGTTCTGAGTTTATTAATAGTGCTTCTGCTGGCAATGAAGTTGGTATAGTTTTGGAGTCTACAAATTTTTATGCTGAGCAAGGTGGTCAGATTTTTGATACTGGATCACTTGAAGGATCCTCTTTCTCGTTTCAAGTTTGCAATGTTCAAATTTATGGAGGTTTTGTTGTCCACATTGGTTCTTTTTCTAGAGAGAGTGGCAAACTCTTAGTCGGTGACAAAGTAAATTGCAAGGTTGATTACAATAGGCGTAAACTCATTGCCCCCAACCATACATGCACGCACATGTTGAATTTTGCTCTGAGGGAAGTTCTTGGAAATCATGTCGACCAGAAGGGTTCTATTGTTCTTGCTGAGAAATTGAGATTTGATTTTTCACATGGCAAGCCGGTAGATCCTGACAGTTTGAGAAGAATAGAGTCAATTGTGAATGAGCAAATCAGAGCTGAATTAGGTGTATATGCTAAAGAGGTAGCCCTGGTTGATGCCAAGCGCATCAATGGTTTAAGGGCTGTATTTGGAGAGGTATATCCTGACCCAGTAAGAGTGGTGGCTATTGGGCGAAAGGTTGAGGATCTTTTGGATTACCCCGAAAATGAGGAATGGTTATCAATTTCAGCAGAACTTTGTGGAGGGACACATATATCAAATACACGGGAAGCAAAGGCCTTTGCTCTTTTATCTGAGGAGGGAATTGCTAAGGGAATACGGAGAATAACTGCTGTCACAAGTGAAGGTGCTTTTCAGGCAATTGAATTGGCTCGGTCGCTTGAGCAAGATGTACATGAAGCGTCCAAGGCTGAAGGAAGCTTGCTGGAGAAGAAAGTAGCTTCCCTAAAATCTCGTGTGGACTCAGCACCAATTCCAGCTGCTAAGAAAGCTGAAATCAGGGCCAAGATGGCTGTACTTCAGACCCAATTGAAGAAAGCACAGAAGATGATTGCGGAGCAAAATATAAAGAATGCTGTAAAGGTTGCAACTGAGATGGCTGAAGTTGCTGCTTCAGATGGGAAAGCTTACTGTGTTTCCCAAGTTGATGTTGGTTTGGATACAGCCGCAGTTCGTGAAGCTGTTTTGAAGATCACTGGGATGGGAATGCCCGCCATGGTTTTCAGCACGGACGAGACCACAAATAAAGCTTTGGTGTGTGCTGGCGTACCAGGGGACAAGGGCACTCAGTTGGAGGTCTCAGAGTGGTTGACAGCAGCCATAGGTCCTTTGAATGGAAGATGCGGAAAAGGAAAAGGTGGCCTTGCTACTGGCCAGGGAACAGATGCATCTCGTATGAATGAGGCGATAGACCTTGCAACGAGTTTTGCACAGATGAAATTGAGATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000049 GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004812 GO:0004813 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005829 GO:0006082 GO:0006139 GO:0006396 GO:0006399 GO:0006400 GO:0006412 GO:0006418 GO:0006419 GO:0006518 GO:0006520 GO:0006725 GO:0006807 GO:0008033 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009451 GO:0009507 GO:0009536 GO:0009987 GO:0010035 GO:0010038 GO:0010467 GO:0016070 GO:0016597 GO:0016874 GO:0016875 GO:0019538 GO:0019752 GO:0031406 GO:0034470 GO:0034641 GO:0034645 GO:0034660 GO:0036094 GO:0042221 GO:0043038 GO:0043039 GO:0043043 GO:0043167 GO:0043168 GO:0043170 GO:0043177 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0046686 GO:0050896 GO:0071704 GO:0090304 GO:0097159 GO:0140098 GO:0140101 GO:1901360 GO:1901363 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00970 map00970

Pfam Domains

Protein Families

DHHA1 tRNA-synt_2c tRNA_SAD

Protein Analysis

1016

Amino Acids

111.54

Weight (kDa)

6.07

Isoelectric Point (pI)

35.2

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
tRNA-synt_2c	PF01411	79 - 652	3.9e-221	tRNA synthetases class II (A)
tRNA_SAD	PF07973	751 - 809	4.4e-14	Threonyl and Alanyl tRNA synthetase second additional domain
DHHA1	PF02272	908 - 1010	2.8e-08	DHHA1 domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0010393)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G50200 AT1G50200
fragaria_vesca	FvH4_4g36780 FvH4_4g36780
malus_domestica	MD13G1007000.v1.1 MD16G1000800.v1.1
prunus_persica	Prupe.1G345900_v2.0.a1
pyrus_communis	pycom13g00590 pycom16g00460
rosa_chinensis	RchiOBHm_Chr4g0446511
rosa_laevigata	RLG00000005653
rosa_multiflora	Rmu_co8401425.1_g000001 Rmu_sc0005526.1_g000002 Rmu_sc0009884.1_g000001
rosa_roxburghii	Rroxscaffold_5G00386930
rosa_rugosa	Rorug04G0372100 Rorug04G0372200
rosa_samantha	Rh4BG442800 Rh4CG460300 Rh4DG441500
rosa_wichuraiana	Rw4G037060

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	585
AasI	GACNNNNNNGTC	1 cut(s)	248
Acc36I	ACCTGC	1 cut(s)	842
AccB7I	CCANNNNNTGG	2 cut(s)	555, 1043
AccBSI	CCGCTC	1 cut(s)	901
AccI	GTMKAC	3 cut(s)	1068, 1769, 2037
AccII	CGCG	6 cut(s)	42, 123, 167, 234, 1164, 1194
AclWI	GGATC	7 cut(s)	308, 821, 1821, 1822, 1834, 2099, 2292
AcoI	YGGCCR	3 cut(s)	221, 1159, 2971
AcsI	RAATTY	8 cut(s)	12, 603, 771, 843, 1661, 1774, 1860, 2002
AcuI	CTGAAG	5 cut(s)	941, 2532, 2603, 2697, 2721
AfaI	GTAC	7 cut(s)	88, 146, 702, 1296, 2493, 2616, 2868
AfiI	CCNNNNNNNGG	3 cut(s)	555, 1043, 2933
AflII	CTTAAG	1 cut(s)	677
AflIII	ACRYGT	1 cut(s)	1995
AjiI	CACGTC	1 cut(s)	277
AjnI	CCWGG	5 cut(s)	1147, 1674, 2187, 2869, 2973
Alw21I	GWGCWC	2 cut(s)	1257, 1567
Alw26I	GTCTC	4 cut(s)	183, 1146, 2828, 2902
Alw44I	GTGCAC	1 cut(s)	1253
AlwI	GGATC	7 cut(s)	308, 821, 1821, 1822, 1834, 2099, 2292
AlwNI	CAGNNNCTG	1 cut(s)	214
Ama87I	CYCGRG	2 cut(s)	408, 1545
AoxI	GGCC	6 cut(s)	221, 1159, 2376, 2601, 2959, 2971
ApaLI	GTGCAC	1 cut(s)	1253
ApeKI	GCWGC	8 cut(s)	140, 589, 803, 1391, 1640, 2579, 2708, 2915
ApoI	RAATTY	8 cut(s)	12, 603, 771, 843, 1661, 1774, 1860, 2002
ArsI	GACNNNNNNTTYG	4 cut(s)	161, 193, 1905, 1937
AseI	ATTAAT	1 cut(s)	1725
Asp700I	GAANNNNTTC	3 cut(s)	350, 1117, 1170
AspLEI	GCGC	6 cut(s)	44, 200, 234, 1182, 1196, 2205
AspS9I	GGNCC	5 cut(s)	749, 758, 1678, 2601, 2924
AsuC2I	CCSGG	1 cut(s)	195
AsuHPI	GGTGA	4 cut(s)	638, 1276, 1289, 1937
AvaI	CYCGRG	2 cut(s)	408, 1545
AvaII	GGWCC	4 cut(s)	749, 758, 1678, 2924
BaeGI	GKGCMC	2 cut(s)	1257, 2885
BaeI	ACNNNNGTAYC	2 cut(s)	972, 1005
BalI	TGGCCA	1 cut(s)	2973
BamHI	GGATCC	1 cut(s)	1826
BanII	GRGCYC	1 cut(s)	1033
BauI	CACGAG	1 cut(s)	2553
BbsI	GAAGAC	2 cut(s)	271, 1361
Bbv12I	GWGCWC	2 cut(s)	1257, 1567
BbvI	GCAGC	8 cut(s)	127, 576, 790, 1378, 1627, 2566, 2695, 2927
BceAI	ACGGC	2 cut(s)	20, 1174
BciT130I	CCWGG	5 cut(s)	1149, 1676, 2189, 2871, 2975
BciVI	GTATCC	1 cut(s)	2752
BcnI	CCSGG	1 cut(s)	195
BcoDI	GTCTC	4 cut(s)	183, 1146, 2828, 2902
BfaI	CTAG	3 cut(s)	291, 663, 1899
BfmI	CTRYAG	1 cut(s)	30
BfoI	RGCGCY	1 cut(s)	201
BfrI	CTTAAG	1 cut(s)	677
BfuAI	ACCTGC	1 cut(s)	842
BfuI	GTATCC	1 cut(s)	2752
BglI	GCCNNNNNGGC	1 cut(s)	2609
BlpI	GCTNAGC	2 cut(s)	909, 1785
Bme1390I	CCNGG	6 cut(s)	195, 1149, 1676, 2189, 2871, 2975
Bme18I	GGWCC	4 cut(s)	749, 758, 1678, 2924
BmeT110I	CYCGRG	2 cut(s)	408, 1545
BmgBI	CACGTC	1 cut(s)	277
BmgT120I	GGNCC	5 cut(s)	749, 758, 1678, 2601, 2924
BmiI	GGNNCC	4 cut(s)	851, 1032, 1673, 1828
BmrFI	CCNGG	6 cut(s)	195, 1149, 1676, 2189, 2871, 2975
BmrI	ACTGGG	3 cut(s)	933, 2243, 2805
BmuI	ACTGGG	3 cut(s)	933, 2243, 2805
BpiI	GAAGAC	2 cut(s)	271, 1361
BpmI	CTGGAG	2 cut(s)	236, 2546
Bpu10I	CCTNAGC	2 cut(s)	1395, 2409
Bpu1102I	GCTNAGC	2 cut(s)	909, 1785
BpuEI	CTTGAG	1 cut(s)	2501
BpuMI	CCSGG	1 cut(s)	195
BsaI	GGTCTC	3 cut(s)	1146, 2828, 2902
BsaJI	CCNNGG	5 cut(s)	2187, 2505, 2814, 2870, 2974
BsaXI	ACNNNNNCTCC	6 cut(s)	146, 176, 1015, 1045, 1860, 1890
Bsc4I	CCNNNNNNNGG	3 cut(s)	555, 1043, 2933
Bse118I	RCCGGY	2 cut(s)	223, 2098
Bse1I	ACTGG	9 cut(s)	219, 349, 751, 928, 1714, 1816, 2249, 2800, 2974
Bse3DI	GCAATG	6 cut(s)	1128, 1159, 1619, 1750, 1858, 1968
BseBI	CCWGG	5 cut(s)	1149, 1676, 2189, 2871, 2975
BseDI	CCNNGG	5 cut(s)	2187, 2505, 2814, 2870, 2974
BseGI	GGATG	9 cut(s)	158, 560, 933, 1066, 1405, 1435, 1543, 1606, 2805
BseLI	CCNNNNNNNGG	3 cut(s)	555, 1043, 2933
BseMI	GCAATG	6 cut(s)	1128, 1159, 1619, 1750, 1858, 1968
BseNI	ACTGG	9 cut(s)	219, 349, 751, 928, 1714, 1816, 2249, 2800, 2974
BseRI	GAGGAG	4 cut(s)	104, 278, 1349, 2411
BseSI	GKGCMC	2 cut(s)	1257, 2885
BseX3I	CGGCCG	1 cut(s)	1159
BseXI	GCAGC	8 cut(s)	127, 576, 790, 1378, 1627, 2566, 2695, 2927
Bsh1236I	CGCG	6 cut(s)	42, 123, 167, 234, 1164, 1194
Bsh1285I	CGRYCG	3 cut(s)	127, 1162, 2477
BshFI	GGCC	6 cut(s)	223, 1161, 2378, 2603, 2961, 2973
BsiEI	CGRYCG	3 cut(s)	127, 1162, 2477
BsiHKAI	GWGCWC	2 cut(s)	1257, 1567
BsiHKCI	CYCGRG	2 cut(s)	408, 1545
BsiSI	CCGG	3 cut(s)	194, 224, 2099
BslFI	GGGAC	6 cut(s)	225, 254, 385, 1460, 2359, 2888
BslI	CCNNNNNNNGG	3 cut(s)	555, 1043, 2933
BsmAI	GTCTC	4 cut(s)	183, 1146, 2828, 2902
BsmBI	CGTCTC	1 cut(s)	183
BsmFI	GGGAC	6 cut(s)	225, 254, 385, 1460, 2359, 2888
BsmI	GAATGC	3 cut(s)	1182, 2677, 2811
BsnI	GGCC	6 cut(s)	223, 1161, 2378, 2603, 2961, 2973
Bso31I	GGTCTC	3 cut(s)	1146, 2828, 2902
BsoBI	CYCGRG	2 cut(s)	408, 1545
Bsp1286I	GDGCHC	4 cut(s)	1033, 1257, 1567, 2885
Bsp1407I	TGTACA	1 cut(s)	2491
Bsp1720I	GCTNAGC	2 cut(s)	909, 1785
Bsp19I	CCATGG	1 cut(s)	2814
BspANI	GGCC	6 cut(s)	223, 1161, 2378, 2603, 2961, 2973
BspFNI	CGCG	6 cut(s)	42, 123, 167, 234, 1164, 1194
BspLI	GGNNCC	4 cut(s)	851, 1032, 1673, 1828
BspMI	ACCTGC	1 cut(s)	842
BspPI	GGATC	7 cut(s)	308, 821, 1821, 1822, 1834, 2099, 2292
BspTI	CTTAAG	1 cut(s)	677
BspTNI	GGTCTC	3 cut(s)	1146, 2828, 2902
BsrBI	CCGCTC	1 cut(s)	901
BsrDI	GCAATG	6 cut(s)	1128, 1159, 1619, 1750, 1858, 1968
BsrFI	RCCGGY	2 cut(s)	223, 2098
BsrGI	TGTACA	1 cut(s)	2491
BsrI	ACTGG	9 cut(s)	219, 349, 751, 928, 1714, 1816, 2249, 2800, 2974
BssAI	RCCGGY	2 cut(s)	223, 2098
BssECI	CCNNGG	5 cut(s)	2187, 2505, 2814, 2870, 2974
BssSI	CACGAG	1 cut(s)	2553
BssT1I	CCWWGG	2 cut(s)	2505, 2814
Bst2BI	CACGAG	1 cut(s)	2553
Bst2UI	CCWGG	5 cut(s)	1149, 1676, 2189, 2871, 2975
Bst4CI	ACNGT	3 cut(s)	708, 2114, 2732
BstAFI	CTTAAG	1 cut(s)	677
BstAUI	TGTACA	1 cut(s)	2491
BstDSI	CCRYGG	1 cut(s)	2814
BstF5I	GGATG	9 cut(s)	158, 560, 933, 1066, 1405, 1435, 1543, 1606, 2805
BstFNI	CGCG	6 cut(s)	42, 123, 167, 234, 1164, 1194
BstH2I	RGCGCY	1 cut(s)	201
BstHHI	GCGC	6 cut(s)	44, 200, 234, 1182, 1196, 2205
BstMAI	GTCTC	4 cut(s)	183, 1146, 2828, 2902
BstMCI	CGRYCG	3 cut(s)	127, 1162, 2477
BstNI	CCWGG	5 cut(s)	1149, 1676, 2189, 2871, 2975
BstNSI	RCATGY	4 cut(s)	417, 917, 1989, 1999
BstSCI	CCNGG	6 cut(s)	193, 1147, 1674, 2187, 2869, 2973
BstSFI	CTRYAG	1 cut(s)	30
BstSLI	GKGCMC	2 cut(s)	1257, 2885
BstUI	CGCG	6 cut(s)	42, 123, 167, 234, 1164, 1194
BstV1I	GCAGC	8 cut(s)	127, 576, 790, 1378, 1627, 2566, 2695, 2927
BstV2I	GAAGAC	2 cut(s)	271, 1361
BstX2I	RGATCY	3 cut(s)	1826, 2104, 2284
BstXI	CCANNNNNNTGG	1 cut(s)	367
BstYI	RGATCY	3 cut(s)	1826, 2104, 2284
BstZI	CGGCCG	1 cut(s)	1159
BsuI	GTATCC	1 cut(s)	2752
BsuRI	GGCC	6 cut(s)	223, 1161, 2378, 2603, 2961, 2973
BtgI	CCRYGG	1 cut(s)	2814
BtrI	CACGTC	1 cut(s)	277
BtsCI	GGATG	9 cut(s)	158, 560, 933, 1066, 1405, 1435, 1543, 1606, 2805
BtsIMutI	CAGTG	5 cut(s)	765, 921, 1268, 1707, 2793
BveI	ACCTGC	1 cut(s)	842
CaiI	CAGNNNCTG	1 cut(s)	214
CfoI	GCGC	6 cut(s)	44, 200, 234, 1182, 1196, 2205
Cfr10I	RCCGGY	2 cut(s)	223, 2098
Cfr13I	GGNCC	5 cut(s)	749, 758, 1678, 2601, 2924
CseI	GACGC	2 cut(s)	223, 2490
Csp6I	GTAC	7 cut(s)	87, 145, 701, 1295, 2492, 2615, 2867
CspCI	CAANNNNNGTGG	2 cut(s)	601, 636
CviQI	GTAC	7 cut(s)	87, 145, 701, 1295, 2492, 2615, 2867
DraI	TTTAAA	1 cut(s)	1660
DrdI	GACNNNNNNGTC	1 cut(s)	248
DseDI	GACNNNNNNGTC	1 cut(s)	248
EaeI	YGGCCR	3 cut(s)	221, 1159, 2971
EagI	CGGCCG	1 cut(s)	1159
EciI	GGCGGA	1 cut(s)	175
EclXI	CGGCCG	1 cut(s)	1159
Eco130I	CCWWGG	2 cut(s)	2505, 2814
Eco147I	AGGCCT	1 cut(s)	2378
Eco24I	GRGCYC	1 cut(s)	1033
Eco31I	GGTCTC	3 cut(s)	1146, 2828, 2902
Eco47I	GGWCC	4 cut(s)	749, 758, 1678, 2924
Eco52I	CGGCCG	1 cut(s)	1159
Eco57I	CTGAAG	5 cut(s)	941, 2532, 2603, 2697, 2721
Eco88I	CYCGRG	2 cut(s)	408, 1545
EcoO109I	RGGNCCY	1 cut(s)	2924
EcoRI	GAATTC	1 cut(s)	12
EcoRII	CCWGG	5 cut(s)	1147, 1674, 2187, 2869, 2973
EcoT14I	CCWWGG	2 cut(s)	2505, 2814
EcoT22I	ATGCAT	1 cut(s)	2989
EcoT38I	GRGCYC	1 cut(s)	1033
ErhI	CCWWGG	2 cut(s)	2505, 2814
Esp3I	CGTCTC	1 cut(s)	183
FalI	AAGNNNNNCTT	6 cut(s)	710, 742, 931, 963, 2946, 2978
FaqI	GGGAC	6 cut(s)	225, 254, 385, 1460, 2359, 2888
FauI	CCCGC	2 cut(s)	437, 2818
FblI	GTMKAC	3 cut(s)	1068, 1769, 2037
FokI	GGATG	9 cut(s)	145, 567, 940, 1073, 1412, 1442, 1550, 1613, 2812
FriOI	GRGCYC	1 cut(s)	1033
FspBI	CTAG	3 cut(s)	291, 663, 1899
GlaI	GCGC	6 cut(s)	43, 199, 233, 1181, 1195, 2204
GsuI	CTGGAG	2 cut(s)	236, 2546
HaeII	RGCGCY	1 cut(s)	201
HaeIII	GGCC	6 cut(s)	223, 1161, 2378, 2603, 2961, 2973
HapII	CCGG	3 cut(s)	194, 224, 2099
HgaI	GACGC	2 cut(s)	223, 2490
HhaI	GCGC	6 cut(s)	44, 200, 234, 1182, 1196, 2205
Hin6I	GCGC	6 cut(s)	42, 198, 232, 1180, 1194, 2203
HinP1I	GCGC	6 cut(s)	42, 198, 232, 1180, 1194, 2203
HincII	GTYRAC	4 cut(s)	817, 919, 2038, 2910
HindII	GTYRAC	4 cut(s)	817, 919, 2038, 2910
HindIII	AAGCTT	3 cut(s)	2518, 2724, 2847
HinfI	GANTC	8 cut(s)	203, 406, 786, 1380, 1474, 1766, 2129, 2561
HpaII	CCGG	3 cut(s)	194, 224, 2099
HphI	GGTGA	4 cut(s)	638, 1276, 1289, 1937
Hpy99I	CGWCG	2 cut(s)	302, 1194
HpyCH4III	ACNGT	3 cut(s)	708, 2114, 2732
HpyCH4IV	ACGT	2 cut(s)	276, 1257
HpySE526I	ACGT	2 cut(s)	276, 1257
HspAI	GCGC	6 cut(s)	42, 198, 232, 1180, 1194, 2203
KroI	GCCGGC	1 cut(s)	223
KroNI	GCCGGC	1 cut(s)	225
LmnI	GCTCC	6 cut(s)	26, 565, 652, 906, 1036, 2656
Lsp1109I	GCAGC	8 cut(s)	127, 576, 790, 1378, 1627, 2566, 2695, 2927
MaeI	CTAG	3 cut(s)	291, 663, 1899
MaeII	ACGT	2 cut(s)	276, 1257
MaeIII	GTNAC	3 cut(s)	1264, 1925, 2434
MbiI	CCGCTC	1 cut(s)	901
MfeI	CAATTG	4 cut(s)	514, 2133, 2460, 2627
MflI	RGATCY	3 cut(s)	1826, 2104, 2284
MhlI	GDGCHC	4 cut(s)	1033, 1257, 1567, 2885
MlsI	TGGCCA	1 cut(s)	2973
MluNI	TGGCCA	1 cut(s)	2973
MlyI	GAGTC	5 cut(s)	400, 1468, 1775, 2138, 2555
MmeI	TCCRAC	2 cut(s)	1039, 2871
Mox20I	TGGCCA	1 cut(s)	2973
Mph1103I	ATGCAT	1 cut(s)	2989
MroNI	GCCGGC	1 cut(s)	223
MroXI	GAANNNNTTC	3 cut(s)	350, 1117, 1170
MscI	TGGCCA	1 cut(s)	2973
MseI	TTAA	5 cut(s)	678, 944, 1659, 1725, 2216
MslI	CAYNNNNRTG	4 cut(s)	926, 1686, 1994, 2798
Msp20I	TGGCCA	1 cut(s)	2973
MspA1I	CMGCKG	3 cut(s)	140, 692, 2579
MspCI	CTTAAG	1 cut(s)	677
MspI	CCGG	3 cut(s)	194, 224, 2099
MspR9I	CCNGG	6 cut(s)	195, 1149, 1676, 2189, 2871, 2975
MunI	CAATTG	4 cut(s)	514, 2133, 2460, 2627
Mva1269I	GAATGC	3 cut(s)	1182, 2677, 2811
MvaI	CCWGG	5 cut(s)	1149, 1676, 2189, 2871, 2975
MvnI	CGCG	6 cut(s)	42, 123, 167, 234, 1164, 1194
NaeI	GCCGGC	1 cut(s)	225
NciI	CCSGG	1 cut(s)	195
NcoI	CCATGG	1 cut(s)	2814
NgoMIV	GCCGGC	1 cut(s)	223
NlaIV	GGNNCC	4 cut(s)	851, 1032, 1673, 1828
NmuCI	GTSAC	3 cut(s)	1264, 1925, 2434
NsiI	ATGCAT	1 cut(s)	2989
NspI	RCATGY	4 cut(s)	417, 917, 1989, 1999
PaeI	GCATGC	1 cut(s)	417
PaeR7I	CTCGAG	2 cut(s)	408, 1545
PceI	AGGCCT	1 cut(s)	2378
PciI	ACATGT	1 cut(s)	1995
PctI	GAATGC	3 cut(s)	1182, 2677, 2811
PdiI	GCCGGC	1 cut(s)	225
PdmI	GAANNNNTTC	3 cut(s)	350, 1117, 1170
PfeI	GAWTC	3 cut(s)	203, 786, 1380
PflMI	CCANNNNNTGG	2 cut(s)	555, 1043
PleI	GAGTC	5 cut(s)	400, 1468, 1774, 2137, 2555
PpsI	GAGTC	5 cut(s)	400, 1468, 1774, 2137, 2555
PpuMI	RGGWCCY	1 cut(s)	2924
PscI	ACATGT	1 cut(s)	1995
PshBI	ATTAAT	1 cut(s)	1725
PsiI	TTATAA	1 cut(s)	585
Psp5II	RGGWCCY	1 cut(s)	2924
Psp6I	CCWGG	5 cut(s)	1147, 1674, 2187, 2869, 2973
PspGI	CCWGG	5 cut(s)	1147, 1674, 2187, 2869, 2973
PspN4I	GGNNCC	4 cut(s)	851, 1032, 1673, 1828
PspPI	GGNCC	5 cut(s)	749, 758, 1678, 2601, 2924
PspPPI	RGGWCCY	1 cut(s)	2924
PspXI	VCTCGAGB	1 cut(s)	408
PstNI	CAGNNNCTG	1 cut(s)	214
PsuI	RGATCY	3 cut(s)	1826, 2104, 2284
PvuII	CAGCTG	2 cut(s)	692, 2579
RsaI	GTAC	7 cut(s)	88, 146, 702, 1296, 2493, 2616, 2868
RsaNI	GTAC	7 cut(s)	87, 145, 701, 1295, 2492, 2615, 2867
RseI	CAYNNNNRTG	4 cut(s)	926, 1686, 1994, 2798
SalI	GTCGAC	1 cut(s)	2036
SaqAI	TTAA	5 cut(s)	678, 944, 1659, 1725, 2216
Sau96I	GGNCC	5 cut(s)	749, 758, 1678, 2601, 2924
SchI	GAGTC	5 cut(s)	400, 1468, 1775, 2138, 2555
ScrFI	CCNGG	6 cut(s)	195, 1149, 1676, 2189, 2871, 2975
SduI	GDGCHC	4 cut(s)	1033, 1257, 1567, 2885
SfcI	CTRYAG	1 cut(s)	30
Sfr274I	CTCGAG	2 cut(s)	408, 1545
SinI	GGWCC	4 cut(s)	749, 758, 1678, 2924
SlaI	CTCGAG	2 cut(s)	408, 1545
SmiMI	CAYNNNNRTG	4 cut(s)	926, 1686, 1994, 2798
SmlI	CTYRAG	4 cut(s)	408, 677, 1545, 2480
SmoI	CTYRAG	4 cut(s)	408, 677, 1545, 2480
SphI	GCATGC	1 cut(s)	417
SseBI	AGGCCT	1 cut(s)	2378
SspMI	CTAG	3 cut(s)	291, 663, 1899
StuI	AGGCCT	1 cut(s)	2378
StyD4I	CCNGG	6 cut(s)	193, 1147, 1674, 2187, 2869, 2973
StyI	CCWWGG	2 cut(s)	2505, 2814
TaaI	ACNGT	3 cut(s)	708, 2114, 2732
TaiI	ACGT	2 cut(s)	279, 1260
TaqI	TCGA	6 cut(s)	261, 409, 462, 601, 1546, 2037
TaqII	GACCGA	1 cut(s)	2463
TatI	WGTACW	4 cut(s)	86, 700, 2491, 2614
TauI	GCSGC	6 cut(s)	106, 109, 140, 901, 1164, 2768
TfiI	GAWTC	3 cut(s)	203, 786, 1380
Tru1I	TTAA	5 cut(s)	678, 944, 1659, 1725, 2216
Tru9I	TTAA	5 cut(s)	678, 944, 1659, 1725, 2216
TscAI	CASTG	5 cut(s)	772, 928, 1275, 1714, 2800
TseFI	GTSAC	3 cut(s)	1264, 1925, 2434
TseI	GCWGC	8 cut(s)	140, 589, 803, 1391, 1640, 2579, 2708, 2915
Tsp45I	GTSAC	3 cut(s)	1264, 1925, 2434
TspGWI	ACGGA	2 cut(s)	2434, 2843
TspRI	CASTG	5 cut(s)	772, 928, 1275, 1714, 2800
Van91I	CCANNNNNTGG	2 cut(s)	555, 1043
Vha464I	CTTAAG	1 cut(s)	677
VneI	GTGCAC	1 cut(s)	1253
VpaK11BI	GGWCC	4 cut(s)	749, 758, 1678, 2924
VspI	ATTAAT	1 cut(s)	1725
XapI	RAATTY	8 cut(s)	12, 603, 771, 843, 1661, 1774, 1860, 2002
XbaI	TCTAGA	1 cut(s)	1898
XceI	RCATGY	4 cut(s)	417, 917, 1989, 1999
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2747
XhoI	CTCGAG	2 cut(s)	408, 1545
XmiI	GTMKAC	3 cut(s)	1068, 1769, 2037
XmnI	GAANNNNTTC	3 cut(s)	350, 1117, 1170
XspI	CTAG	3 cut(s)	291, 663, 1899
Zsp2I	ATGCAT	1 cut(s)	2989

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.