pycom16g00600

ADP-ribosylation factor GTPase-activating protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0007229
Physical Location & Seq
Forward (+)
368229 .. 371335
3107 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1371 bp
ATGGGCGGCTCCCAGTCACATCATTTCACGCGCTCACCGGAACATCACAGTGGTGTGTTTAGCAGATTTCGTTCAAGACATAGGGTCGCGTCACTTAATGAAAACATTCTGGGCTGCCGTACTGTTGATCTGCGTACCTCAACAATAAAGATGGACGCAGAGGATACAGATCTTCGACTTTGCTTTAGAATAATATCCCCATTGAAAACTTACACATTGCAGGCTGAAAATGAAGCTGATAGGATGGATTGGACAAACAAAATAGCAGGAGTTATTGCATCTCTTTTGAATTTTCAGCTTCTTGACCAGCCACATCCTGGAACTGTGAATCACGAGAGTAGTAAATCTTCTCTTGGTACTTATGATATTCGTAGAGTGAAAAGCACTGAAAGTTTAGGAAATACCAGAAAATTCAACAGAGTAGATTCTGTTTCTAGTATTCTCAGGAGAATTCCTGGAAATGATCTTTGTGCGGAGTGTGGTGCTCCTGAGCCTGACTGGGCATCTCTTAATCTTGGCGTACTGATGTGCATCGAATGTTCTGGTGTTCACCGGAATCTTGGTGTACACATTTCAAAGGTGAGATCTCTAACATTAGATGTCAAGGTTTGGGAACCTATACTTGTGGACTTATTTCGCAACTTGGGTAATGCATATTGTAATTCTGTATGGGAGGGAACGCTTCTACTTGAGAATGAAAAACTGGATGGTTCGAAGGACATGAGGGCTCCAGTTTTAAAACCAGGCCCCAAGGATGCAGCTCAGCATAAGGAAATATACATTCAAGCAAAGTATGTAGAGAAAGTGATGGTTATTAGAGATGTAGCTGCATCGGGCATGCCTGTTCTTGCATCAGATATATGGGAAGCAGTCAAGGCTGGTAATTTACAAGAAGTCTACCGCATTATCGTGATGTCAGATGTGAATATTGTCGACACCGCCTTTGAGAATGTGGTTACTCATGATTTATATCACCATGCTGAAGCAATACAGTCGGAGTCGGATTTTAACACAATGGAGATGAAACTGTATGATCCAGCAGCGTGTGATAGAATCAAGGAGTCCAATGAGCCTGGGAACTGTCTTCAAGGTTGTTCATTATTGCATCTGGCTTGTCACTCCGGCAATCCGATGATGGTTGAATTGCTGCTGCAGTTTGGTGCCAACATGAATTTGCGTGATTTCCATGGAAGGACTCCTTTGCACCACTGCATCACCAGCGGGAACAACTCATTGGCAAAGTGGCTACTTAGGAGGGGTGCACGCCCGTCAATTCAAGATGGGGGAGGGCAAAGTGCATTAGAAAGAGCGATGGAGATGGGAGCAATTACAGACGAGGAGTTGTTCATACTACTTTCTGAACACGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
Ank Ank_2 Ank_3 Ank_5 ArfGap BAR_3 PH

Protein Analysis

457

Amino Acids

50.84

Weight (kDa)

6.03

Isoelectric Point (pI)

45.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ArfGap PF01412 146 - 271 2.8e-31 Putative GTPase activating protein for Arf
Ank_2 PF12796 354 - 427 2.7e-13 Ankyrin repeats (3 copies)
Ank_5 PF13857 360 - 403 1.7e-06 Ankyrin repeats (many copies)
Ank PF00023 364 - 394 2e-07 Ankyrin repeat
Ank_4 PF13637 368 - 417 1.1e-08 Ankyrin repeats (many copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1160
AccB7I CCANNNNNTGG 1 cut(s) 317
AccI GTMKAC 2 cut(s) 897, 933
AccII CGCG 2 cut(s) 31, 89
AciI CCGC 5 cut(s) 6, 473, 901, 939, 1221
AclWI GGATC 1 cut(s) 1028
AcsI RAATTY 4 cut(s) 289, 410, 450, 1171
AcuI CTGAAG 1 cut(s) 1002
AfaI GTAC 5 cut(s) 121, 136, 358, 522, 567
AfiI CCNNNNNNNGG 1 cut(s) 317
AgsI TTSAA 9 cut(s) 75, 205, 289, 415, 576, 785, 1088, 1142, 1277
AhdI GACNNNNNGTC 1 cut(s) 83
AjnI CCWGG 4 cut(s) 316, 454, 742, 1072
AjuI GAANNNNNNNTTGG 2 cut(s) 1217, 1249
AleI CACNNNNGTG 1 cut(s) 51
AluBI AGCT 4 cut(s) 236, 298, 761, 827
AluI AGCT 4 cut(s) 236, 298, 761, 827
Alw21I GWGCWC 2 cut(s) 487, 1264
Alw44I GTGCAC 1 cut(s) 1260
AlwI GGATC 1 cut(s) 1028
AoxI GGCC 1 cut(s) 745
ApaLI GTGCAC 1 cut(s) 1260
ApeKI GCWGC 6 cut(s) 114, 758, 827, 1040, 1147, 1150
ApoI RAATTY 4 cut(s) 289, 410, 450, 1171
ArsI GACNNNNNNTTYG 2 cut(s) 926, 958
Asp700I GAANNNNTTC 1 cut(s) 105
AspLEI GCGC 1 cut(s) 33
AspS9I GGNCC 1 cut(s) 746
AsuHPI GGTGA 5 cut(s) 27, 542, 592, 965, 1207
AsuII TTCGAA 1 cut(s) 713
BaeGI GKGCMC 1 cut(s) 1264
BanI GGYRCC 1 cut(s) 1160
BanII GRGCYC 1 cut(s) 730
BarI GAAGNNNNNNTAC 2 cut(s) 331, 363
BauI CACGAG 2 cut(s) 332, 1364
BbsI GAAGAC 1 cut(s) 1076
Bbv12I GWGCWC 2 cut(s) 487, 1264
BbvI GCAGC 6 cut(s) 101, 770, 814, 1052, 1134, 1137
BccI CCATC 8 cut(s) 145, 238, 701, 802, 1129, 1274, 1306, 1312
BceAI ACGGC 1 cut(s) 102
BcgI CGANNNNNNTGC 2 cut(s) 975, 1009
BciT130I CCWGG 4 cut(s) 318, 456, 744, 1074
BciVI GTATCC 1 cut(s) 157
BfaI CTAG 1 cut(s) 435
BfmI CTRYAG 1 cut(s) 1151
BfuI GTATCC 1 cut(s) 157
BglII AGATCT 2 cut(s) 169, 584
BisI GCNGC 7 cut(s) 7, 115, 759, 828, 1041, 1148, 1151
BlpI GCTNAGC 1 cut(s) 762
BlsI GCNGC 7 cut(s) 8, 116, 760, 829, 1042, 1149, 1152
Bme1390I CCNGG 4 cut(s) 318, 456, 744, 1074
BmeRI GACNNNNNGTC 1 cut(s) 83
BmgT120I GGNCC 1 cut(s) 746
BmiI GGNNCC 5 cut(s) 10, 615, 729, 748, 1162
BmrFI CCNGG 4 cut(s) 318, 456, 744, 1074
BmrI ACTGGG 2 cut(s) 7, 508
BmsI GCATC 8 cut(s) 287, 512, 540, 745, 839, 860, 1114, 1221
BmuI ACTGGG 2 cut(s) 7, 508
BpiI GAAGAC 1 cut(s) 1076
BpmI CTGGAG 1 cut(s) 714
Bpu10I CCTNAGC 1 cut(s) 489
Bpu1102I GCTNAGC 1 cut(s) 762
Bpu14I TTCGAA 1 cut(s) 713
BpuEI CTTGAG 1 cut(s) 710
BsaBI GATNNNNATC 3 cut(s) 168, 530, 969
BsaJI CCNNGG 3 cut(s) 750, 1073, 1186
BsaWI WCCGGW 2 cut(s) 37, 552
Bsc4I CCNNNNNNNGG 1 cut(s) 317
Bse1I ACTGG 4 cut(s) 13, 503, 708, 731
Bse3DI GCAATG 1 cut(s) 215
Bse8I GATNNNNATC 3 cut(s) 168, 530, 969
BseBI CCWGG 4 cut(s) 318, 456, 744, 1074
BseDI CCNNGG 3 cut(s) 750, 1073, 1186
BseGI GGATG 4 cut(s) 249, 313, 712, 760
BseJI GATNNNNATC 3 cut(s) 168, 530, 969
BseLI CCNNNNNNNGG 1 cut(s) 317
BseMI GCAATG 1 cut(s) 215
BseMII CTCAG 3 cut(s) 457, 480, 776
BseNI ACTGG 4 cut(s) 13, 503, 708, 731
BseRI GAGGAG 1 cut(s) 1352
BseSI GKGCMC 1 cut(s) 1264
BseXI GCAGC 6 cut(s) 101, 770, 814, 1052, 1134, 1137
Bsh1236I CGCG 2 cut(s) 31, 89
BshFI GGCC 1 cut(s) 747
BshNI GGYRCC 1 cut(s) 1160
BsiHKAI GWGCWC 2 cut(s) 487, 1264
BsiSI CCGG 3 cut(s) 38, 553, 1122
BslI CCNNNNNNNGG 1 cut(s) 317
BsnI GGCC 1 cut(s) 747
Bsp119I TTCGAA 1 cut(s) 713
Bsp1286I GDGCHC 3 cut(s) 487, 730, 1264
Bsp1407I TGTACA 1 cut(s) 565
Bsp143I GATC 5 cut(s) 127, 169, 463, 584, 1033
Bsp1720I GCTNAGC 1 cut(s) 762
Bsp19I CCATGG 1 cut(s) 1186
BspACI CCGC 5 cut(s) 6, 473, 901, 939, 1221
BspANI GGCC 1 cut(s) 747
BspCNI CTCAG 3 cut(s) 456, 481, 775
BspFNI CGCG 2 cut(s) 31, 89
BspHI TCATGA 1 cut(s) 961
BspLI GGNNCC 5 cut(s) 10, 615, 729, 748, 1162
BspMAI CTGCAG 1 cut(s) 1155
BspPI GGATC 1 cut(s) 1028
BspT104I TTCGAA 1 cut(s) 713
BspT107I GGYRCC 1 cut(s) 1160
BsrDI GCAATG 1 cut(s) 215
BsrGI TGTACA 1 cut(s) 565
BsrI ACTGG 4 cut(s) 13, 503, 708, 731
BssECI CCNNGG 3 cut(s) 750, 1073, 1186
BssMI GATC 5 cut(s) 127, 169, 463, 584, 1033
BssSI CACGAG 2 cut(s) 332, 1364
BssT1I CCWWGG 2 cut(s) 750, 1186
Bst2BI CACGAG 2 cut(s) 332, 1364
Bst2UI CCWGG 4 cut(s) 318, 456, 744, 1074
Bst4CI ACNGT 6 cut(s) 50, 124, 325, 993, 1029, 1082
BstAUI TGTACA 1 cut(s) 565
BstBI TTCGAA 1 cut(s) 713
BstC8I GCNNGC 3 cut(s) 222, 839, 1264
BstDEI CTNAG 4 cut(s) 443, 489, 762, 1250
BstDSI CCRYGG 1 cut(s) 1186
BstF5I GGATG 4 cut(s) 249, 313, 712, 760
BstFNI CGCG 2 cut(s) 31, 89
BstHHI GCGC 1 cut(s) 33
BstKTI GATC 5 cut(s) 130, 172, 466, 587, 1036
BstMBI GATC 5 cut(s) 127, 169, 463, 584, 1033
BstMWI GCNNNNNNNGC 2 cut(s) 875, 1218
BstNI CCWGG 4 cut(s) 318, 456, 744, 1074
BstNSI RCATGY 1 cut(s) 841
BstSCI CCNGG 4 cut(s) 316, 454, 742, 1072
BstSFI CTRYAG 1 cut(s) 1151
BstSLI GKGCMC 1 cut(s) 1264
BstUI CGCG 2 cut(s) 31, 89
BstV1I GCAGC 6 cut(s) 101, 770, 814, 1052, 1134, 1137
BstV2I GAAGAC 1 cut(s) 1076
BstX2I RGATCY 2 cut(s) 169, 584
BstYI RGATCY 2 cut(s) 169, 584
BsuI GTATCC 1 cut(s) 157
BsuRI GGCC 1 cut(s) 747
BtgI CCRYGG 1 cut(s) 1186
BtgZI GCGATG 1 cut(s) 1325
BtsCI GGATG 4 cut(s) 249, 313, 712, 760
BtsI GCAGTG 1 cut(s) 1207
BtsIMutI CAGTG 3 cut(s) 55, 384, 1207
Cac8I GCNNGC 3 cut(s) 222, 839, 1264
CciI TCATGA 1 cut(s) 961
CfoI GCGC 1 cut(s) 33
Cfr13I GGNCC 1 cut(s) 746
CseI GACGC 2 cut(s) 78, 164
Csp6I GTAC 5 cut(s) 120, 135, 357, 521, 566
CviAII CATG 6 cut(s) 721, 838, 962, 977, 1168, 1187
CviQI GTAC 5 cut(s) 120, 135, 357, 521, 566
DdeI CTNAG 4 cut(s) 443, 489, 762, 1250
DpnI GATC 5 cut(s) 129, 171, 465, 586, 1035
DpnII GATC 5 cut(s) 127, 169, 463, 584, 1033
DraI TTTAAA 1 cut(s) 738
DriI GACNNNNNGTC 1 cut(s) 83
Eam1105I GACNNNNNGTC 1 cut(s) 83
Eco130I CCWWGG 2 cut(s) 750, 1186
Eco24I GRGCYC 1 cut(s) 730
Eco57I CTGAAG 1 cut(s) 1002
EcoO109I RGGNCCY 1 cut(s) 746
EcoRI GAATTC 1 cut(s) 450
EcoRII CCWGG 4 cut(s) 316, 454, 742, 1072
EcoT14I CCWWGG 2 cut(s) 750, 1186
EcoT22I ATGCAT 1 cut(s) 655
EcoT38I GRGCYC 1 cut(s) 730
ErhI CCWWGG 2 cut(s) 750, 1186
FaeI CATG 6 cut(s) 724, 841, 965, 980, 1171, 1190
FalI AAGNNNNNCTT 2 cut(s) 1183, 1215
FatI CATG 6 cut(s) 720, 837, 961, 976, 1167, 1186
FauI CCCGC 1 cut(s) 1214
FblI GTMKAC 2 cut(s) 897, 933
Fnu4HI GCNGC 7 cut(s) 7, 115, 759, 828, 1041, 1148, 1151
FokI GGATG 4 cut(s) 256, 300, 719, 767
FriOI GRGCYC 1 cut(s) 730
Fsp4HI GCNGC 7 cut(s) 7, 115, 759, 828, 1041, 1148, 1151
FspBI CTAG 1 cut(s) 435
GlaI GCGC 1 cut(s) 32
GluI GCNGC 7 cut(s) 7, 115, 759, 828, 1041, 1148, 1151
GsuI CTGGAG 1 cut(s) 714
HaeIII GGCC 1 cut(s) 747
HapII CCGG 3 cut(s) 38, 553, 1122
HgaI GACGC 2 cut(s) 78, 164
HhaI GCGC 1 cut(s) 33
Hin1II CATG 6 cut(s) 724, 841, 965, 980, 1171, 1190
Hin6I GCGC 1 cut(s) 31
HinP1I GCGC 1 cut(s) 31
HincII GTYRAC 1 cut(s) 934
HindII GTYRAC 1 cut(s) 934
HinfI GANTC 7 cut(s) 328, 425, 556, 998, 1053, 1061, 1195
HpaII CCGG 3 cut(s) 38, 553, 1122
HphI GGTGA 5 cut(s) 27, 542, 592, 965, 1207
Hpy166II GTNNAC 7 cut(s) 550, 566, 568, 628, 898, 934, 1262
Hpy188I TCNGA 6 cut(s) 856, 919, 997, 1003, 1131, 1360
Hpy188III TCNNGA 8 cut(s) 75, 302, 332, 445, 488, 910, 962, 1277
Hpy8I GTNNAC 7 cut(s) 550, 566, 568, 628, 898, 934, 1262
HpyAV CCTTC 2 cut(s) 709, 1185
HpyCH4III ACNGT 6 cut(s) 50, 124, 325, 993, 1029, 1082
HpyF10VI GCNNNNNNNGC 2 cut(s) 875, 1218
HpyF3I CTNAG 4 cut(s) 443, 489, 762, 1250
Hsp92II CATG 6 cut(s) 724, 841, 965, 980, 1171, 1190
HspAI GCGC 1 cut(s) 31
Kzo9I GATC 5 cut(s) 127, 169, 463, 584, 1033
LmnI GCTCC 4 cut(s) 14, 490, 733, 1322
Lsp1109I GCAGC 6 cut(s) 101, 770, 814, 1052, 1134, 1137
LweI GCATC 8 cut(s) 287, 512, 540, 745, 839, 860, 1114, 1221
MaeI CTAG 1 cut(s) 435
MaeIII GTNAC 4 cut(s) 15, 90, 955, 1115
MalI GATC 5 cut(s) 129, 171, 465, 586, 1035
MboI GATC 5 cut(s) 127, 169, 463, 584, 1033
MboII GAAGA 3 cut(s) 164, 339, 1076
MflI RGATCY 2 cut(s) 169, 584
MhlI GDGCHC 3 cut(s) 487, 730, 1264
MluCI AATT 9 cut(s) 289, 410, 450, 661, 883, 1142, 1171, 1272, 1326
MlyI GAGTC 3 cut(s) 1007, 1070, 1189
MmeI TCCRAC 2 cut(s) 975, 981
MnlI CCTC 7 cut(s) 148, 154, 667, 717, 1248, 1280, 1330
Mph1103I ATGCAT 1 cut(s) 655
MroXI GAANNNNTTC 1 cut(s) 105
MseI TTAA 4 cut(s) 96, 510, 737, 1008
MslI CAYNNNNRTG 3 cut(s) 48, 51, 908
MspA1I CMGCKG 1 cut(s) 1221
MspI CCGG 3 cut(s) 38, 553, 1122
MspR9I CCNGG 4 cut(s) 318, 456, 744, 1074
MvaI CCWGG 4 cut(s) 318, 456, 744, 1074
MvnI CGCG 2 cut(s) 31, 89
MwoI GCNNNNNNNGC 2 cut(s) 875, 1218
NcoI CCATGG 1 cut(s) 1186
NdeII GATC 5 cut(s) 127, 169, 463, 584, 1033
NlaIII CATG 6 cut(s) 724, 841, 965, 980, 1171, 1190
NlaIV GGNNCC 5 cut(s) 10, 615, 729, 748, 1162
NmuCI GTSAC 3 cut(s) 15, 90, 1115
NsiI ATGCAT 1 cut(s) 655
NspI RCATGY 1 cut(s) 841
NspV TTCGAA 1 cut(s) 713
OliI CACNNNNGTG 1 cut(s) 51
PaeI GCATGC 1 cut(s) 841
PagI TCATGA 1 cut(s) 961
PdmI GAANNNNTTC 1 cut(s) 105
PfeI GAWTC 4 cut(s) 328, 425, 556, 1053
PflMI CCANNNNNTGG 1 cut(s) 317
PfoI TCCNGGA 2 cut(s) 316, 454
PkrI GCNGC 7 cut(s) 8, 116, 760, 829, 1042, 1149, 1152
PleI GAGTC 3 cut(s) 1006, 1069, 1189
PpsI GAGTC 3 cut(s) 1006, 1069, 1189
Psp6I CCWGG 4 cut(s) 316, 454, 742, 1072
PspGI CCWGG 4 cut(s) 316, 454, 742, 1072
PspN4I GGNNCC 5 cut(s) 10, 615, 729, 748, 1162
PspPI GGNCC 1 cut(s) 746
PstI CTGCAG 1 cut(s) 1155
PsuI RGATCY 2 cut(s) 169, 584
RsaI GTAC 5 cut(s) 121, 136, 358, 522, 567
RsaNI GTAC 5 cut(s) 120, 135, 357, 521, 566
RseI CAYNNNNRTG 3 cut(s) 48, 51, 908
SalI GTCGAC 1 cut(s) 932
SaqAI TTAA 4 cut(s) 96, 510, 737, 1008
SatI GCNGC 7 cut(s) 7, 115, 759, 828, 1041, 1148, 1151
Sau3AI GATC 5 cut(s) 127, 169, 463, 584, 1033
Sau96I GGNCC 1 cut(s) 746
SchI GAGTC 3 cut(s) 1007, 1070, 1189
ScrFI CCNGG 4 cut(s) 318, 456, 744, 1074
SduI GDGCHC 3 cut(s) 487, 730, 1264
SetI ASST 9 cut(s) 140, 238, 300, 582, 609, 619, 763, 829, 1093
SfaNI GCATC 8 cut(s) 287, 512, 540, 745, 839, 860, 1114, 1221
SfcI CTRYAG 1 cut(s) 1151
SfuI TTCGAA 1 cut(s) 713
SmiMI CAYNNNNRTG 3 cut(s) 48, 51, 908
SmlI CTYRAG 1 cut(s) 689
SmoI CTYRAG 1 cut(s) 689
SphI GCATGC 1 cut(s) 841
Sse9I AATT 9 cut(s) 289, 410, 450, 661, 883, 1142, 1171, 1272, 1326
SsiI CCGC 5 cut(s) 6, 473, 901, 939, 1221
SspI AATATT 1 cut(s) 928
SspMI CTAG 1 cut(s) 435
StyD4I CCNGG 4 cut(s) 316, 454, 742, 1072
StyI CCWWGG 2 cut(s) 750, 1186
TaaI ACNGT 6 cut(s) 50, 124, 325, 993, 1029, 1082
TaqI TCGA 4 cut(s) 175, 534, 713, 933
TasI AATT 9 cut(s) 289, 410, 450, 661, 883, 1142, 1171, 1272, 1326
TatI WGTACW 1 cut(s) 565
TauI GCSGC 1 cut(s) 9
TfiI GAWTC 4 cut(s) 328, 425, 556, 1053
Tru1I TTAA 4 cut(s) 96, 510, 737, 1008
Tru9I TTAA 4 cut(s) 96, 510, 737, 1008
TscAI CASTG 3 cut(s) 55, 391, 1214
TseFI GTSAC 3 cut(s) 15, 90, 1115
TseI GCWGC 6 cut(s) 114, 758, 827, 1040, 1147, 1150
Tsp45I GTSAC 3 cut(s) 15, 90, 1115
TspDTI ATGAA 7 cut(s) 114, 246, 711, 1037, 1086, 1184, 1336
TspRI CASTG 3 cut(s) 55, 391, 1214
Van91I CCANNNNNTGG 1 cut(s) 317
VneI GTGCAC 1 cut(s) 1260
XapI RAATTY 4 cut(s) 289, 410, 450, 1171
XceI RCATGY 1 cut(s) 841
XcmI CCANNNNNNNNNTGG 1 cut(s) 314
XmiI GTMKAC 2 cut(s) 897, 933
XmnI GAANNNNTTC 1 cut(s) 105
XspI CTAG 1 cut(s) 435
Zsp2I ATGCAT 1 cut(s) 655
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.