pycom16g02990

ATP-dependent RNA helicase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0005622
Physical Location & Seq
Reverse (-)
1884060 .. 1890060
6001 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2637 bp
ATGCCCTCTGTTTTCTTCCTCCTCCTCCTCCCATCTCCTCCGTCTCTGCAATTCCCTTGCGGGCCGTCGCCTTCGCATCCAAAACCCATTGCCTCCTCCTCCAAACCCTCACTCCTGGCGATGAAGGACCGGCCTCCTTCCTCGTACGGCTCCGTCTATGTCCCTCCCCACCACCGCCTCCGCTCCGTCATCAGCACCCCCAATTATACCTCCCCTGCTTCAATCGGCTCCAAGCTCCGCGAAAACCAGACTGCTGCTGCTGCTCTCAACCGGAGGAGCAGCACCAATGGTGCTCTGGCATACTACCAGACTCAGCAGCAGCAGCTGCAGCAACAGGAGCAGTTTCAGAAGCCCAAGCTTCAGCACACTTCTGCTTACGACGACGGCGTTTCCGAGGAAGGCTCCGATCGCGAAGTTGAGTTACCGTCGCATCCGACACTGAGTCCTTACTCATCTGATATTGATGAGTGGAAACGGAAATTAACCATGCTGTTACGTGACAAGATGAAGCAAGAGTTGGTTACGAGGGAGAAAAAAGATAGACGTGACTTTGATAAAATAGCTGCTTTGGCAAGCAGAATGGGGCTTTATAGCTATCTGTATTCGAAAGTTGCTGTGTTTAGTAAGGTTCCACTGCCAAACTACAGATTTGACCTGGATGATAGGCGCCCTCAGAGAGAGGTGACCTTGCCTCTTGGGTTGCTGAGGAGAGTTGAGAGCTATCTTGGAGAGTTCCTTTCCCAGAAGTCTAGGACAAGGGAAAGCTTTCAAAATCTTTCCTTTTCTAGATCGAACAGTAGTGGCAGCATTGCTACTGACGAAGGGCTTTTTGAGCAACCAGAATCATTTGCGTCGAGTAAGGGTGTGATGGAAAAACTTCTTTGGCGGAGAAGTACGCAGCTGCGCGATAAGCAACAAGCTTGGCAGGAATCTCCTGAAGGGAAAAAAATGCTTGAACTTCGTAGAAGTCTTCCTGCTTATAAAGAGAAGGATGCATTATTGGCAGCAATTTCACGGGATCAGGTCGTTATCATATCAGGTGAAACTGGCTGTGGCAAGACTACTCAAATTCCACAATTCATTTTAGAATCTGAGATAGAAGCTGTTCGTGGAGCTGTTTGTAGTATTATATGTACACAGCCTAGACGGATATCTGCTATGTCTGTTTCTGAAAGAGTTGCTTCAGAGAGAGGAGAGAAATTGGGTGACTCGGTTGGGTATAAGGTTCGGTTAGAAGGTATGAAGGGAAGGGATACTCGCCTTCTCTTTTGCACCACTGGCATTTTGTTGAGAAGATTACTAGTTGATAGAAATTTGAAAGGTGTAACTCATGTGATTGTGGATGAAATTCATGAACGTGGAATGAATGAAGATTTTCTGCTGATTGTTCTCAAGGATCTCCTTCCTCAGCGACCAGAACTGAGGTTGATTTTGATGAGTGCAACCCTAGATTCTGAACTTTTCTCATCCTACTTTGGTAGGGCTCAGATAATTCATGTTCCAGGTTTTACATATCCAGTTCGGACTCATTTTCTGGAGGATGTTCTGGAAATTACGGGATGCAAATTAACTCCATACAATCAGATTGATGATTATGGCCAAGAAAAGATGTGGAAAATGAGCAAACAGGCACCAAGAAAGAGGAAAAGCCAAATTGTTTCTGTTGTTGAGGATGCACTTAAAGCAGCCGATTTTAAGGGGCATAGTCCACAGACTCGGGAGTCTCTAGCATGTTGGAATCCTGATAGTATCGGTTTTTATCTGATTGAGTATCTCTTGTGCAATATCTGTGAGAGCGACAAGCCTGGTGCCATTCTAGTTTTTATGACTGGGTGGGATGACATCAATTCTCTGAAGGAAAAGTTACATGCCAATCCTCTATTAGGCGATCCAAGCCGAGTCTTGTTACTAGCATGCCATGGTTCTATGGCAAGTACTGAGCAGAGGCTAATATTTGATGAGCCTGGGGATGGAGTGAGGAAAATAGTGCTGGCTACTAATATTGCTGAGACAAGTATCACAATTAATGATGTTGTTTTTGTACTTGATTGTGGAAAGGCAAAAGAGACTTCATATGACGCACTGAACAATACTCCTTGTTTGCTTCCTTCCTGGATTTCCAAGGTTTCTGCTCAACAAAGAAGAGGAAGAGCAGGACGTGTCCAGCCAGGGGAGTGTTATCATCTCTATCCTAGATGTGTCTATGATGCATTTGCAGAGTATCAATTGCCAGAGATTTTGAGGACACCTCTGCAGTCTCTCTGTCTGCAAATAAAAAGTTTAAACCTCGGAAGCATTTCTGAGTTCTTATCTAGGGCTCTGCAGTCACCAGAATTACTCGCGGTGCAAAACGCCATAGAGTATTTAAAGATCATCGGGGCCTTGGATGAGAATGAAAATCTGACTGTTTTAGGACGCTATCTAACTATGCTTCCTGTTGAGCCAAAACTTGGAAAGATGCTCTTGGTAGGTGCCATCTTCAACTGCCTGGATCCGGTTTTGACTATTGTTTCTGGCCTTAGTGTCAGAGATCCTTTCCTTACACCATTTGACAAGAAAGATCTTGCTGAGGCTGCAAAATCTCAGTTCTCCCGGGACAATAGTGATCACCTTGTATGCACTTGTGCGGGCCTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000976 GO:0000977 GO:0000978 GO:0000987 GO:0001012 GO:0001047 GO:0001067 GO:0001503 GO:0001817 GO:0001819 GO:0002151 GO:0002791 GO:0002793 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003697 GO:0003723 GO:0003724 GO:0003725 GO:0003824 GO:0004004 GO:0004386 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006139 GO:0006355 GO:0006357 GO:0006396 GO:0006725 GO:0006807 GO:0008026 GO:0008094 GO:0008150 GO:0008152 GO:0008186 GO:0009605 GO:0009607 GO:0009615 GO:0009889 GO:0009891 GO:0009892 GO:0009893 GO:0009894 GO:0009895 GO:0009987 GO:0010033 GO:0010467 GO:0010468 GO:0010501 GO:0010556 GO:0010557 GO:0010604 GO:0010605 GO:0010608 GO:0010628 GO:0010638 GO:0014070 GO:0016070 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019219 GO:0019222 GO:0019899 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031328 GO:0031329 GO:0031330 GO:0032204 GO:0032206 GO:0032479 GO:0032481 GO:0032501 GO:0032647 GO:0032727 GO:0032879 GO:0032880 GO:0033043 GO:0033044 GO:0034641 GO:0042221 GO:0042623 GO:0042826 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043330 GO:0043331 GO:0043487 GO:0043489 GO:0043565 GO:0044212 GO:0044237 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0045893 GO:0045934 GO:0045935 GO:0045944 GO:0046483 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050707 GO:0050708 GO:0050714 GO:0050715 GO:0050789 GO:0050794 GO:0050896 GO:0051046 GO:0051047 GO:0051049 GO:0051050 GO:0051052 GO:0051054 GO:0051128 GO:0051130 GO:0051171 GO:0051172 GO:0051173 GO:0051222 GO:0051223 GO:0051239 GO:0051240 GO:0051252 GO:0051253 GO:0051254 GO:0051704 GO:0051707 GO:0051880 GO:0060255 GO:0065007 GO:0065008 GO:0070034 GO:0070035 GO:0070201 GO:0071704 GO:0080090 GO:0090087 GO:0090304 GO:0090669 GO:0097159 GO:0140098 GO:1901360 GO:1901363 GO:1901698 GO:1902369 GO:1902680 GO:1902739 GO:1902741 GO:1903506 GO:1903508 GO:1903530 GO:1903532 GO:1904951 GO:1990837 GO:2000112 GO:2001141 GO:2001252
KEGG Pathways
Metabolic & Signaling
ko03018 map03018
Pfam Domains
Protein Families
DEAD HA2 Helicase_C OB_NTP_bind dsrm

Protein Analysis

879

Amino Acids

98.51

Weight (kDa)

8.53

Isoelectric Point (pI)

55.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DEAD PF00270 334 - 486 1.4e-10 DEAD/DEAH box helicase
Helicase_C PF00271 593 - 721 7.5e-11 Helicase conserved C-terminal domain
WHD_HA2 PF04408 785 - 813 9.7e-08 Helicase associated domain (HA2), winged-helix
HA2_C PF21010 814 - 872 8.4e-16 Helicase associated domain (HA2), ratchet-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 981
AasI GACNNNNNNGTC 1 cut(s) 1720
AccB1I GGYRCC 4 cut(s) 666, 1630, 1808, 2471
AccB7I CCANNNNNTGG 1 cut(s) 2450
AccBSI CCGCTC 1 cut(s) 183
AccII CGCG 4 cut(s) 240, 411, 906, 2342
AciI CCGC 7 cut(s) 60, 175, 181, 238, 886, 2342, 2627
AclWI GGATC 6 cut(s) 1026, 1404, 1883, 2486, 2499, 2525
AcoI YGGCCR 1 cut(s) 1597
AcsI RAATTY 3 cut(s) 1068, 1312, 1347
AcuI CTGAAG 4 cut(s) 344, 957, 1167, 1874
AcyI GRCGYC 1 cut(s) 667
AfaI GTAC 5 cut(s) 146, 895, 1135, 1936, 2043
AfiI CCNNNNNNNGG 5 cut(s) 1883, 1970, 2170, 2450, 2494
AflIII ACRYGT 1 cut(s) 2158
AgsI TTSAA 5 cut(s) 222, 770, 956, 1318, 2482
AhdI GACNNNNNGTC 1 cut(s) 441
AhlI ACTAGT 1 cut(s) 1300
AjiI CACGTC 2 cut(s) 545, 2159
AjnI CCWGG 8 cut(s) 114, 654, 1501, 1804, 1963, 2111, 2167, 2487
AjuI GAANNNNNNNTTGG 2 cut(s) 500, 532
Alw21I GWGCWC 1 cut(s) 295
Alw26I GTCTC 5 cut(s) 48, 1728, 2003, 2060, 2262
AlwI GGATC 6 cut(s) 1026, 1404, 1883, 2486, 2499, 2525
AlwNI CAGNNNCTG 1 cut(s) 325
Ama87I CYCGRG 2 cut(s) 1716, 2592
AoxI GGCC 6 cut(s) 62, 131, 1597, 2379, 2515, 2629
ApoI RAATTY 3 cut(s) 1068, 1312, 1347
AseI ATTAAT 1 cut(s) 2025
Asp700I GAANNNNTTC 5 cut(s) 765, 876, 1104, 1374, 2296
AspLEI GCGC 2 cut(s) 669, 906
AspS9I GGNCC 4 cut(s) 62, 127, 2379, 2629
AsuC2I CCSGG 2 cut(s) 2593, 2594
AsuHPI GGTGA 5 cut(s) 694, 1052, 1217, 2319, 2600
AsuII TTCGAA 1 cut(s) 605
AvaI CYCGRG 2 cut(s) 1716, 2592
AvaII GGWCC 1 cut(s) 127
BalI TGGCCA 1 cut(s) 1599
BamHI GGATCC 1 cut(s) 2491
BanI GGYRCC 4 cut(s) 666, 1630, 1808, 2471
BanII GRGCYC 2 cut(s) 1486, 2320
BarI GAAGNNNNNNTAC 2 cut(s) 405, 437
BbsI GAAGAC 1 cut(s) 962
Bbv12I GWGCWC 1 cut(s) 295
BbvCI CCTCAGC 3 cut(s) 704, 1407, 2568
BccI CCATC 4 cut(s) 40, 862, 1964, 2483
BceAI ACGGC 3 cut(s) 49, 163, 400
BciT130I CCWGG 8 cut(s) 116, 656, 1503, 1806, 1965, 2113, 2169, 2489
BciVI GTATCC 1 cut(s) 1246
BclI TGATCA 1 cut(s) 2605
BcnI CCSGG 2 cut(s) 2593, 2594
BcoDI GTCTC 5 cut(s) 48, 1728, 2003, 2060, 2262
BcuI ACTAGT 1 cut(s) 1300
BfmI CTRYAG 4 cut(s) 326, 643, 2252, 2321
BfoI RGCGCY 1 cut(s) 670
BfuI GTATCC 1 cut(s) 1246
BglII AGATCT 1 cut(s) 2560
BmcAI AGTACT 1 cut(s) 1936
Bme18I GGWCC 1 cut(s) 127
BmeRI GACNNNNNGTC 1 cut(s) 441
BmeT110I CYCGRG 2 cut(s) 1716, 2592
BmgBI CACGTC 2 cut(s) 545, 2159
BmgT120I GGNCC 4 cut(s) 62, 127, 2379, 2629
BmrI ACTGGG 1 cut(s) 1839
BmsI GCATC 7 cut(s) 85, 439, 982, 1550, 1663, 2197, 2448
BmuI ACTGGG 1 cut(s) 1839
BpiI GAAGAC 1 cut(s) 962
BplI GAGNNNNNCTC 4 cut(s) 386, 418, 2233, 2265
BpmI CTGGAG 1 cut(s) 1556
Bpu10I CCTNAGC 3 cut(s) 704, 1407, 2568
Bpu14I TTCGAA 1 cut(s) 605
BpuEI CTTGAG 1 cut(s) 1376
BpuMI CCSGG 2 cut(s) 2593, 2594
BsaAI YACGTR 1 cut(s) 497
BsaHI GRCGYC 1 cut(s) 667
BsaJI CCNNGG 8 cut(s) 393, 1918, 1964, 2121, 2168, 2287, 2382, 2592
BsaWI WCCGGW 2 cut(s) 270, 2494
BsaXI ACNNNNNCTCC 2 cut(s) 96, 126
Bsc4I CCNNNNNNNGG 5 cut(s) 1883, 1970, 2170, 2450, 2494
Bse118I RCCGGY 1 cut(s) 129
Bse1I ACTGG 4 cut(s) 1051, 1282, 1517, 1834
Bse3DI GCAATG 2 cut(s) 87, 807
BseBI CCWGG 8 cut(s) 116, 656, 1503, 1806, 1965, 2113, 2169, 2489
BseDI CCNNGG 8 cut(s) 393, 1918, 1964, 2121, 2168, 2287, 2382, 2592
BseLI CCNNNNNNNGG 5 cut(s) 1883, 1970, 2170, 2450, 2494
BseMI GCAATG 2 cut(s) 87, 807
BseNI ACTGG 4 cut(s) 1051, 1282, 1517, 1834
BseRI GAGGAG 9 cut(s) 11, 14, 17, 27, 85, 88, 289, 721, 1206
Bsh1236I CGCG 4 cut(s) 240, 411, 906, 2342
Bsh1285I CGRYCG 1 cut(s) 409
BshFI GGCC 6 cut(s) 64, 133, 1599, 2381, 2517, 2631
BshNI GGYRCC 4 cut(s) 666, 1630, 1808, 2471
BsiEI CGRYCG 1 cut(s) 409
BsiHKAI GWGCWC 1 cut(s) 295
BsiHKCI CYCGRG 2 cut(s) 1716, 2592
BsiSI CCGG 4 cut(s) 130, 271, 2495, 2593
BsiWI CGTACG 1 cut(s) 144
BslFI GGGAC 2 cut(s) 146, 2609
BslI CCNNNNNNNGG 5 cut(s) 1883, 1970, 2170, 2450, 2494
BsmAI GTCTC 5 cut(s) 48, 1728, 2003, 2060, 2262
BsmBI CGTCTC 1 cut(s) 48
BsmFI GGGAC 2 cut(s) 146, 2609
BsnI GGCC 6 cut(s) 64, 133, 1599, 2381, 2517, 2631
BsoBI CYCGRG 2 cut(s) 1716, 2592
Bsp119I TTCGAA 1 cut(s) 605
Bsp1286I GDGCHC 3 cut(s) 295, 1486, 2320
Bsp1407I TGTACA 1 cut(s) 1133
Bsp19I CCATGG 1 cut(s) 1918
Bsp68I TCGCGA 1 cut(s) 411
BspACI CCGC 7 cut(s) 60, 175, 181, 238, 886, 2342, 2627
BspANI GGCC 6 cut(s) 64, 133, 1599, 2381, 2517, 2631
BspFNI CGCG 4 cut(s) 240, 411, 906, 2342
BspHI TCATGA 1 cut(s) 1351
BspMAI CTGCAG 3 cut(s) 330, 2256, 2325
BspPI GGATC 6 cut(s) 1026, 1404, 1883, 2486, 2499, 2525
BspQI GCTCTTC 1 cut(s) 2143
BspT104I TTCGAA 1 cut(s) 605
BspT107I GGYRCC 4 cut(s) 666, 1630, 1808, 2471
BsrBI CCGCTC 1 cut(s) 183
BsrDI GCAATG 2 cut(s) 87, 807
BsrFI RCCGGY 1 cut(s) 129
BsrGI TGTACA 1 cut(s) 1133
BsrI ACTGG 4 cut(s) 1051, 1282, 1517, 1834
BssAI RCCGGY 1 cut(s) 129
BssECI CCNNGG 8 cut(s) 393, 1918, 1964, 2121, 2168, 2287, 2382, 2592
BssNI GRCGYC 1 cut(s) 667
BssT1I CCWWGG 3 cut(s) 1918, 2121, 2382
Bst2UI CCWGG 8 cut(s) 116, 656, 1503, 1806, 1965, 2113, 2169, 2489
Bst4CI ACNGT 3 cut(s) 426, 797, 2407
Bst6I CTCTTC 2 cut(s) 2137, 2143
BstACI GRCGYC 1 cut(s) 667
BstAPI GCANNNNNTGC 1 cut(s) 325
BstAUI TGTACA 1 cut(s) 1133
BstBAI YACGTR 1 cut(s) 497
BstBI TTCGAA 1 cut(s) 605
BstC8I GCNNGC 5 cut(s) 62, 574, 1915, 1992, 2629
BstDSI CCRYGG 1 cut(s) 1918
BstEII GGTNACC 1 cut(s) 682
BstENI CCTNNNNNAGG 1 cut(s) 1881
BstFNI CGCG 4 cut(s) 240, 411, 906, 2342
BstH2I RGCGCY 1 cut(s) 670
BstHHI GCGC 2 cut(s) 669, 906
BstMAI GTCTC 5 cut(s) 48, 1728, 2003, 2060, 2262
BstMCI CGRYCG 1 cut(s) 409
BstNI CCWGG 8 cut(s) 116, 656, 1503, 1806, 1965, 2113, 2169, 2489
BstNSI RCATGY 3 cut(s) 1734, 1871, 1917
BstPI GGTNACC 1 cut(s) 682
BstSFI CTRYAG 4 cut(s) 326, 643, 2252, 2321
BstUI CGCG 4 cut(s) 240, 411, 906, 2342
BstV2I GAAGAC 1 cut(s) 962
BstX2I RGATCY 4 cut(s) 1396, 2491, 2530, 2560
BstYI RGATCY 4 cut(s) 1396, 2491, 2530, 2560
BsuI GTATCC 1 cut(s) 1246
BsuRI GGCC 6 cut(s) 64, 133, 1599, 2381, 2517, 2631
BtgI CCRYGG 1 cut(s) 1918
BtgZI GCGATG 1 cut(s) 134
BtrI CACGTC 2 cut(s) 545, 2159
BtsI GCAGTG 1 cut(s) 632
BtsIMutI CAGTG 4 cut(s) 437, 632, 1275, 2081
BtuMI TCGCGA 1 cut(s) 411
Cac8I GCNNGC 5 cut(s) 62, 574, 1915, 1992, 2629
CaiI CAGNNNCTG 1 cut(s) 325
CciI TCATGA 1 cut(s) 1351
CfoI GCGC 2 cut(s) 669, 906
Cfr10I RCCGGY 1 cut(s) 129
Cfr13I GGNCC 4 cut(s) 62, 127, 2379, 2629
Cfr9I CCCGGG 1 cut(s) 2592
CseI GACGC 3 cut(s) 840, 2087, 2424
Csp6I GTAC 5 cut(s) 145, 894, 1134, 1935, 2042
CviAII CATG 8 cut(s) 487, 1331, 1352, 1496, 1731, 1868, 1914, 1919
CviQI GTAC 5 cut(s) 145, 894, 1134, 1935, 2042
DinI GGCGCC 1 cut(s) 668
DraI TTTAAA 2 cut(s) 2283, 2367
DrdI GACNNNNNNGTC 1 cut(s) 1720
DriI GACNNNNNGTC 1 cut(s) 441
DseDI GACNNNNNNGTC 1 cut(s) 1720
EaeI YGGCCR 1 cut(s) 1597
Eam1104I CTCTTC 2 cut(s) 2137, 2143
Eam1105I GACNNNNNGTC 1 cut(s) 441
EarI CTCTTC 2 cut(s) 2137, 2143
EciI GGCGGA 1 cut(s) 901
Eco130I CCWWGG 3 cut(s) 1918, 2121, 2382
Eco24I GRGCYC 2 cut(s) 1486, 2320
Eco32I GATATC 1 cut(s) 1152
Eco47I GGWCC 1 cut(s) 127
Eco57I CTGAAG 4 cut(s) 344, 957, 1167, 1874
Eco88I CYCGRG 2 cut(s) 1716, 2592
Eco91I GGTNACC 1 cut(s) 682
EcoNI CCTNNNNNAGG 1 cut(s) 1881
EcoO109I RGGNCCY 1 cut(s) 2379
EcoO65I GGTNACC 1 cut(s) 682
EcoRII CCWGG 8 cut(s) 114, 654, 1501, 1804, 1963, 2111, 2167, 2487
EcoRV GATATC 1 cut(s) 1152
EcoT14I CCWWGG 3 cut(s) 1918, 2121, 2382
EcoT22I ATGCAT 2 cut(s) 997, 2212
EcoT38I GRGCYC 2 cut(s) 1486, 2320
EgeI GGCGCC 1 cut(s) 668
EheI GGCGCC 1 cut(s) 668
ErhI CCWWGG 3 cut(s) 1918, 2121, 2382
Esp3I CGTCTC 1 cut(s) 48
FaeI CATG 8 cut(s) 490, 1334, 1355, 1499, 1734, 1871, 1917, 1922
FalI AAGNNNNNCTT 4 cut(s) 1165, 1197, 2447, 2479
FaqI GGGAC 2 cut(s) 146, 2609
FatI CATG 8 cut(s) 486, 1330, 1351, 1495, 1730, 1867, 1913, 1918
FauI CCCGC 2 cut(s) 53, 2620
FauNDI CATATG 1 cut(s) 2074
FbaI TGATCA 1 cut(s) 2605
FriOI GRGCYC 2 cut(s) 1486, 2320
GlaI GCGC 2 cut(s) 668, 905
GsuI CTGGAG 1 cut(s) 1556
HaeII RGCGCY 1 cut(s) 670
HaeIII GGCC 6 cut(s) 64, 133, 1599, 2381, 2517, 2631
HapII CCGG 4 cut(s) 130, 271, 2495, 2593
HgaI GACGC 3 cut(s) 840, 2087, 2424
HhaI GCGC 2 cut(s) 669, 906
Hin1I GRCGYC 1 cut(s) 667
Hin1II CATG 8 cut(s) 490, 1334, 1355, 1499, 1734, 1871, 1917, 1922
Hin6I GCGC 2 cut(s) 667, 904
HinP1I GCGC 2 cut(s) 667, 904
HindIII AAGCTT 3 cut(s) 356, 763, 918
HpaII CCGG 4 cut(s) 130, 271, 2495, 2593
HphI GGTGA 5 cut(s) 694, 1052, 1217, 2319, 2600
Hpy166II GTNNAC 2 cut(s) 1136, 1709
Hpy188III TCNNGA 8 cut(s) 410, 786, 935, 1352, 1535, 1547, 1718, 1742
Hpy8I GTNNAC 2 cut(s) 1136, 1709
Hpy99I CGWCG 5 cut(s) 70, 383, 386, 430, 856
HpyCH4III ACNGT 3 cut(s) 426, 797, 2407
HpyCH4IV ACGT 4 cut(s) 496, 544, 1357, 2158
HpySE526I ACGT 4 cut(s) 496, 544, 1357, 2158
Hsp92I GRCGYC 1 cut(s) 667
Hsp92II CATG 8 cut(s) 490, 1334, 1355, 1499, 1734, 1871, 1917, 1922
HspAI GCGC 2 cut(s) 667, 904
KasI GGCGCC 1 cut(s) 666
Ksp22I TGATCA 1 cut(s) 2605
LguI GCTCTTC 1 cut(s) 2143
LmnI GCTCC 8 cut(s) 155, 188, 233, 240, 276, 337, 407, 1112
LweI GCATC 7 cut(s) 85, 439, 982, 1550, 1663, 2197, 2448
MaeII ACGT 4 cut(s) 496, 544, 1357, 2158
MbiI CCGCTC 1 cut(s) 183
MboII GAAGA 7 cut(s) 7, 962, 1305, 1382, 2154, 2160, 2470
MfeI CAATTG 1 cut(s) 2225
MflI RGATCY 4 cut(s) 1396, 2491, 2530, 2560
MhlI GDGCHC 3 cut(s) 295, 1486, 2320
MlsI TGGCCA 1 cut(s) 1599
MluNI TGGCCA 1 cut(s) 1599
Mly113I GGCGCC 1 cut(s) 667
MlyI GAGTC 7 cut(s) 304, 451, 1202, 1519, 1708, 1730, 1908
MmeI TCCRAC 2 cut(s) 458, 1715
Mox20I TGGCCA 1 cut(s) 1599
Mph1103I ATGCAT 2 cut(s) 997, 2212
MroXI GAANNNNTTC 5 cut(s) 765, 876, 1104, 1374, 2296
MscI TGGCCA 1 cut(s) 1599
MseI TTAA 7 cut(s) 482, 1568, 1680, 1695, 2025, 2282, 2366
MslI CAYNNNNRTG 1 cut(s) 1356
Msp20I TGGCCA 1 cut(s) 1599
MspA1I CMGCKG 2 cut(s) 325, 901
MspI CCGG 4 cut(s) 130, 271, 2495, 2593
MssI GTTTAAAC 1 cut(s) 2283
MunI CAATTG 1 cut(s) 2225
MvaI CCWGG 8 cut(s) 116, 656, 1503, 1806, 1965, 2113, 2169, 2489
MvnI CGCG 4 cut(s) 240, 411, 906, 2342
NarI GGCGCC 1 cut(s) 667
NciI CCSGG 2 cut(s) 2593, 2594
NcoI CCATGG 1 cut(s) 1918
NdeI CATATG 1 cut(s) 2074
NlaIII CATG 8 cut(s) 490, 1334, 1355, 1499, 1734, 1871, 1917, 1922
NmeAIII GCCGAG 1 cut(s) 1922
NmuCI GTSAC 5 cut(s) 497, 545, 682, 1205, 2325
NruI TCGCGA 1 cut(s) 411
NsiI ATGCAT 2 cut(s) 997, 2212
NspI RCATGY 3 cut(s) 1734, 1871, 1917
NspV TTCGAA 1 cut(s) 605
PaeI GCATGC 1 cut(s) 1917
PagI TCATGA 1 cut(s) 1351
PciSI GCTCTTC 1 cut(s) 2143
PcsI WCGNNNNNNNCGW 2 cut(s) 384, 390
PdmI GAANNNNTTC 5 cut(s) 765, 876, 1104, 1374, 2296
PfeI GAWTC 5 cut(s) 842, 929, 1088, 1451, 1738
Pfl23II CGTACG 1 cut(s) 144
PflMI CCANNNNNTGG 1 cut(s) 2450
PfoI TCCNGGA 1 cut(s) 2111
Ple19I CGATCG 1 cut(s) 409
PleI GAGTC 7 cut(s) 304, 450, 1202, 1519, 1708, 1729, 1907
PluTI GGCGCC 1 cut(s) 670
PmeI GTTTAAAC 1 cut(s) 2283
PpsI GAGTC 7 cut(s) 304, 450, 1202, 1519, 1708, 1729, 1907
Ppu21I YACGTR 1 cut(s) 497
PshBI ATTAAT 1 cut(s) 2025
PsiI TTATAA 1 cut(s) 981
Psp6I CCWGG 8 cut(s) 114, 654, 1501, 1804, 1963, 2111, 2167, 2487
PspEI GGTNACC 1 cut(s) 682
PspGI CCWGG 8 cut(s) 114, 654, 1501, 1804, 1963, 2111, 2167, 2487
PspLI CGTACG 1 cut(s) 144
PspPI GGNCC 4 cut(s) 62, 127, 2379, 2629
PstI CTGCAG 3 cut(s) 330, 2256, 2325
PstNI CAGNNNCTG 1 cut(s) 325
PsuI RGATCY 4 cut(s) 1396, 2491, 2530, 2560
PvuI CGATCG 1 cut(s) 409
PvuII CAGCTG 2 cut(s) 325, 901
RruI TCGCGA 1 cut(s) 411
RsaI GTAC 5 cut(s) 146, 895, 1135, 1936, 2043
RsaNI GTAC 5 cut(s) 145, 894, 1134, 1935, 2042
RseI CAYNNNNRTG 1 cut(s) 1356
SapI GCTCTTC 1 cut(s) 2143
SaqAI TTAA 7 cut(s) 482, 1568, 1680, 1695, 2025, 2282, 2366
Sau96I GGNCC 4 cut(s) 62, 127, 2379, 2629
ScaI AGTACT 1 cut(s) 1936
SchI GAGTC 7 cut(s) 304, 451, 1202, 1519, 1708, 1730, 1908
SduI GDGCHC 3 cut(s) 295, 1486, 2320
SfaNI GCATC 7 cut(s) 85, 439, 982, 1550, 1663, 2197, 2448
SfcI CTRYAG 4 cut(s) 326, 643, 2252, 2321
SfoI GGCGCC 1 cut(s) 668
SfuI TTCGAA 1 cut(s) 605
SinI GGWCC 1 cut(s) 127
SmaI CCCGGG 1 cut(s) 2594
SmiMI CAYNNNNRTG 1 cut(s) 1356
SmlI CTYRAG 1 cut(s) 1391
SmoI CTYRAG 1 cut(s) 1391
SpeI ACTAGT 1 cut(s) 1300
SphI GCATGC 1 cut(s) 1917
SsiI CCGC 7 cut(s) 60, 175, 181, 238, 886, 2342, 2627
SspDI GGCGCC 1 cut(s) 666
SspI AATATT 2 cut(s) 1953, 2002
StyI CCWWGG 3 cut(s) 1918, 2121, 2382
TaaI ACNGT 3 cut(s) 426, 797, 2407
TaiI ACGT 4 cut(s) 499, 547, 1360, 2161
TaqI TCGA 3 cut(s) 605, 791, 854
TatI WGTACW 3 cut(s) 1133, 1934, 2041
TfiI GAWTC 5 cut(s) 842, 929, 1088, 1451, 1738
Tru1I TTAA 7 cut(s) 482, 1568, 1680, 1695, 2025, 2282, 2366
Tru9I TTAA 7 cut(s) 482, 1568, 1680, 1695, 2025, 2282, 2366
TscAI CASTG 4 cut(s) 444, 639, 1282, 2088
TseFI GTSAC 5 cut(s) 497, 545, 682, 1205, 2325
Tsp45I GTSAC 5 cut(s) 497, 545, 682, 1205, 2325
TspGWI ACGGA 5 cut(s) 30, 142, 175, 490, 1162
TspMI CCCGGG 1 cut(s) 2592
TspRI CASTG 4 cut(s) 444, 639, 1282, 2088
Van91I CCANNNNNTGG 1 cut(s) 2450
VpaK11BI GGWCC 1 cut(s) 127
VspI ATTAAT 1 cut(s) 2025
XagI CCTNNNNNAGG 1 cut(s) 1881
XapI RAATTY 3 cut(s) 1068, 1312, 1347
XbaI TCTAGA 1 cut(s) 785
XceI RCATGY 3 cut(s) 1734, 1871, 1917
XcmI CCANNNNNNNNNTGG 1 cut(s) 292
XmaI CCCGGG 1 cut(s) 2592
XmnI GAANNNNTTC 5 cut(s) 765, 876, 1104, 1374, 2296
ZrmI AGTACT 1 cut(s) 1936
Zsp2I ATGCAT 2 cut(s) 997, 2212
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.