pycom16g03030

PKHD-type hydroxylase At1g22950-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0004916
Physical Location & Seq
Forward (+)
1905794 .. 1911777
5984 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2589 bp
ATGAAGAAAGCATCTGATTTTATATGTTGTTCTTGGCAGCATTCTGCATACCTCTTGGCTACTCAGTTCGCCACACTGATGAAGCGTGAAGGGTACTTCCTATCTAGTTATCAAGTAGAGCCGATTTCTCCCAAGGCTGATGGGGGCTTCAGCAGTCAGTGGCATAGTGCTAGTATTAGTGCCACTCCATCAGCTCGACCAATCGTCCTACCTTCCTCAACTCTGACCCCAAGGTTATCACCTTCAGGACTTAGTCCCATATCCATGCAACGCCTTTCACAGAACATTTTCTCTGGAGGTCAATTTCTCCCACCTGAAATCATACAGTTAGCTGCACAACTCCCGGGCACTTGTATACCCAAGCAAGCTCTCAGCATTGCTACACAAGAAAATTGCATGATGCCGCAAGTCCGGCAGATTCTGAATAATTGTGCCTATTCAATGTATCAGACGCTGCAAATACAACAACAACAACGAAATCAATTCATGCAGGGGGGATCAGGAGGTGGAGGAGTAACTCCAAGTGGTTTAGGTATGATTGAACGGGGTGGAGTCCAGGGACTTGGAAATTCTCGTCTTGGTTTGTTGGGTAATGGCATGGGCTTGAGGTCCTCTACTCCTACGGAATTGGGAGGACGGATGCCTTGGGTTGGCAATGTAGGTCAGTTTAACAATCTTGGCAGTGAAATATCTGGTTTAAATGACAGCAAGCTGCCTCTTGGCCCGGTTTCTGATCATCTGAGGGCCGCAGAAGGCCAAGGGAAAGCCTTGAGGACTGGGGTTTCGGTGGACATATCAAATTTTCCAGGCGTGACTAGTAAATTAAGCAACCAGCAGCTGCTACAATTGCAGATGCATCCACTGGAAATGAGATCACCGTTTGAACAGCAGATACAACCGGAAATTAGATCACTGGTTGAGCAGAAGATACTGCCACAGATGCATCCACCGGAAGTGGAATCATCAGTCCAGCAGCAGATACAACCGCAGATGTATCAAGCGGAAATGAGATCACCAGTTGGGCAGCAGCTACAACCGCAGATGTATCAACCAGGACTGGGATCACCAGTTGAGCTGCAGATGCAACCGCAGATGCATCAACAGGAATTGGGATCACCAGTCCAACAGCGGATGCAACCGGGTATACACATCCATTTTGTCTTTTTGAAAGAAAATGTAAGGGAAAACAATGACCCTCATGTTTCTTCAATGTTTTTCTTCTACCTATTTGTTTCATGTGGGAGAATGGTGTGCCAAGTATATGTTGCCAAAACAAGATCGACTGTCCACTTACGTGGTTGGAGCAGTGGAACCCAAGCCCTAACCTGCAGCTCAAACTTCTTCTTCTTCATTTTTCATTTCTTCAAAACCCTAACAAAATTCCACCAATTCAGTCACTATATCTCTCTGAAAATCCCCAATCTCTCTCACGAAGAGTCGAAGACTATGGCTACGGACGGCGCGGCGGACCGACGGAGGCCACCGCCGCAAACGCTGAGAACAGGCAACAACGGAAACTGCGTGATGAGCTTGTCGAACAGGCTGAGACTGATGCCGAACAAGGATCACAAGGCGGAGAGCTACGAGGACCTGGAGCAGTTGGAGTTCAGTCCCTTGCTCTTCAGCTCGCTGGAGCGCTACTTGCCTCCCACCATGCTCAATGCCTCACGTGACCTCAAGCTCCAGTACATGAGGGACATTCTCCACCGGTACTCACCCGAGAGCGAACGCACTCGTGTTCAACGACATCGAGAATACAGGCAGAAGATCATCACACACTATCAGCCTCTAAAAAGGGAGTTGTACACTCTGCAAGCTGAAAAGTTCTTTGTCCCCTCATTTCTCAGAGCAATCAGTGAGAACACAGAGGATAGCTTTAGAAGTATACTGTCTGAACCCTCTCCTGGAGTTTATACATTTGAAATGCTTCAGCCGGATTTCTGTGAATTGTTAGTAACTGAGGTGGAAAATTTTCAAGGGTGGGTCAATGAGACAAATTTCAGAATCATGCGACCAAATACAATGAATAGATACGGTTGTGTTCTTGATGACTTTGGCCTGGAAACCATGCTTGACAAGTTGATGGAGGACTTTATATGTCCTATTTCCAAAGTTTTCTTCCCGGAAGTTGGTGGATCCACACTGGATACTCATCACGGTTTTGTTGTTGAATATGGAATTGATAGGGATGTAGAGCTTGGTTTTCATGTGGATGATTCAGAAGTCACCTTGAATGTTTGCTTGGGAAAGCAATTTTCAGGTGGAGATTTGTTCTTTCGAGGTGTTCGATGTGATAAACATGTTAATTCAGAGACACAATCTGAGGAAATCTTTGATTATCCTCATGTCCCGGGACATGCCATTCTTCATCGTGGTCGCCATCGACATGGTGCTAGAGCCACGACATCAGGGCGTCGAGTCAACTTACTATTATGGTGCAGAAGTTCGGTCTTTAGGGAGCTGAAGAAATATCAGAAAGATTTTTCCAACTGGTGTGGAGAGTGCAAACGTGAGAAGAAAGAAAGGCAGCGCCAATCAATTGCCGCAACCAAACTGGAAATACTTAAGAGAGATGGACAAACTGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

863

Amino Acids

97.68

Weight (kDa)

8.61

Isoelectric Point (pI)

56.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
OGFOD2-like PF25238 590 - 712 1.4e-54 OGFOD2-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1334
AccI GTMKAC 3 cut(s) 355, 1144, 1885
AccII CGCG 1 cut(s) 1463
AclWI GGATC 6 cut(s) 505, 1069, 1120, 1572, 2130, 2143
AcsI RAATTY 5 cut(s) 568, 799, 1379, 1969, 1996
AcuI CTGAAG 5 cut(s) 133, 228, 1606, 1913, 2483
AcvI CACGTG 1 cut(s) 1670
AcyI GRCGYC 1 cut(s) 2413
AfaI GTAC 4 cut(s) 95, 1688, 1712, 1805
AfeI AGCGCT 1 cut(s) 1637
AfiI CCNNNNNNNGG 4 cut(s) 650, 1058, 1904, 2129
AflII CTTAAG 1 cut(s) 2564
AflIII ACRYGT 1 cut(s) 2299
AgeI ACCGGT 1 cut(s) 1707
AhdI GACNNNNNGTC 1 cut(s) 203
AhlI ACTAGT 1 cut(s) 815
AjnI CCWGG 6 cut(s) 555, 805, 1051, 1590, 1903, 2058
AjuI GAANNNNNNNTTGG 4 cut(s) 2102, 2134, 2225, 2257
AleI CACNNNNGTG 1 cut(s) 1293
Alw26I GTCTC 3 cut(s) 1540, 1985, 2306
AlwI GGATC 6 cut(s) 505, 1069, 1120, 1572, 2130, 2143
AlwNI CAGNNNCTG 3 cut(s) 421, 454, 838
Ama87I CYCGRG 3 cut(s) 343, 1718, 2350
Aor51HI AGCGCT 1 cut(s) 1637
AoxI GGCC 5 cut(s) 721, 744, 754, 1478, 2056
ApoI RAATTY 5 cut(s) 568, 799, 1379, 1969, 1996
ArsI GACNNNNNNTTYG 2 cut(s) 766, 798
AsiGI ACCGGT 1 cut(s) 1707
Asp700I GAANNNNTTC 3 cut(s) 287, 1926, 1971
AspLEI GCGC 3 cut(s) 1463, 1638, 2532
AspS9I GGNCC 5 cut(s) 609, 722, 744, 1468, 1588
AsuC2I CCSGG 7 cut(s) 344, 345, 725, 1140, 2123, 2351, 2352
AsuHPI GGTGA 7 cut(s) 231, 867, 1005, 1056, 1107, 1707, 2218
AvaI CYCGRG 3 cut(s) 343, 1718, 2350
AvaII GGWCC 3 cut(s) 609, 1468, 1588
BaeGI GKGCMC 1 cut(s) 350
BaeI ACNNNNGTAYC 2 cut(s) 2023, 2056
BamHI GGATCC 1 cut(s) 2135
BauI CACGAG 1 cut(s) 1734
BbrPI CACGTG 1 cut(s) 1670
BbsI GAAGAC 1 cut(s) 1448
BccI CCATC 5 cut(s) 134, 196, 2077, 2388, 2567
BceAI ACGGC 1 cut(s) 1474
BciT130I CCWGG 6 cut(s) 557, 807, 1053, 1592, 1905, 2060
BciVI GTATCC 1 cut(s) 2140
BclI TGATCA 1 cut(s) 733
BcnI CCSGG 7 cut(s) 344, 345, 725, 1140, 2123, 2351, 2352
BcoDI GTCTC 3 cut(s) 1540, 1985, 2306
BcuI ACTAGT 1 cut(s) 815
BfaI CTAG 4 cut(s) 105, 171, 816, 2394
BfmI CTRYAG 2 cut(s) 1076, 1327
BfoI RGCGCY 2 cut(s) 1639, 2533
BfrI CTTAAG 1 cut(s) 2564
BfuAI ACCTGC 1 cut(s) 1334
BfuI GTATCC 1 cut(s) 2140
Bme18I GGWCC 3 cut(s) 609, 1468, 1588
BmeRI GACNNNNNGTC 1 cut(s) 203
BmeT110I CYCGRG 3 cut(s) 343, 1718, 2350
BmgT120I GGNCC 5 cut(s) 609, 722, 744, 1468, 1588
BmiI GGNNCC 2 cut(s) 1312, 2137
BmrI ACTGGG 2 cut(s) 786, 1067
BmuI ACTGGG 2 cut(s) 786, 1067
BpiI GAAGAC 1 cut(s) 1448
BpmI CTGGAG 5 cut(s) 315, 1613, 1652, 1667, 1926
BpuEI CTTGAG 3 cut(s) 625, 790, 1661
BpuMI CCSGG 7 cut(s) 344, 345, 725, 1140, 2123, 2351, 2352
BsaAI YACGTR 2 cut(s) 1295, 1670
BsaBI GATNNNNATC 1 cut(s) 2151
BsaHI GRCGYC 1 cut(s) 2413
BsaJI CCNNGG 7 cut(s) 132, 230, 343, 556, 644, 757, 2350
BsaWI WCCGGW 3 cut(s) 898, 949, 1707
Bsc4I CCNNNNNNNGG 4 cut(s) 650, 1058, 1904, 2129
Bse118I RCCGGY 1 cut(s) 1707
Bse3DI GCAATG 2 cut(s) 375, 661
Bse8I GATNNNNATC 1 cut(s) 2151
BseBI CCWGG 6 cut(s) 557, 807, 1053, 1592, 1905, 2060
BseDI CCNNGG 7 cut(s) 132, 230, 343, 556, 644, 757, 2350
BseGI GGATG 7 cut(s) 645, 856, 943, 1137, 1149, 2194, 2218
BseJI GATNNNNATC 1 cut(s) 2151
BseLI CCNNNNNNNGG 4 cut(s) 650, 1058, 1904, 2129
BseMI GCAATG 2 cut(s) 375, 661
BseMII CTCAG 8 cut(s) 77, 385, 731, 1487, 1535, 1858, 1950, 2313
BseRI GAGGAG 1 cut(s) 525
BseSI GKGCMC 1 cut(s) 350
BsgI GTGCAG 2 cut(s) 318, 2458
Bsh1236I CGCG 1 cut(s) 1463
BshFI GGCC 5 cut(s) 723, 746, 756, 1480, 2058
BshTI ACCGGT 1 cut(s) 1707
BsiHKCI CYCGRG 3 cut(s) 343, 1718, 2350
BslFI GGGAC 7 cut(s) 240, 573, 1596, 1709, 1817, 2333, 2367
BslI CCNNNNNNNGG 4 cut(s) 650, 1058, 1904, 2129
BsmAI GTCTC 3 cut(s) 1540, 1985, 2306
BsmFI GGGAC 7 cut(s) 240, 573, 1596, 1709, 1817, 2333, 2367
BsmI GAATGC 1 cut(s) 40
BsnI GGCC 5 cut(s) 723, 746, 756, 1480, 2058
BsoBI CYCGRG 3 cut(s) 343, 1718, 2350
Bsp1286I GDGCHC 1 cut(s) 350
Bsp1407I TGTACA 1 cut(s) 1803
BspANI GGCC 5 cut(s) 723, 746, 756, 1480, 2058
BspCNI CTCAG 8 cut(s) 76, 384, 732, 1488, 1536, 1857, 1951, 2314
BspFNI CGCG 1 cut(s) 1463
BspLI GGNNCC 2 cut(s) 1312, 2137
BspMAI CTGCAG 2 cut(s) 1080, 1331
BspMI ACCTGC 1 cut(s) 1334
BspPI GGATC 6 cut(s) 505, 1069, 1120, 1572, 2130, 2143
BspQI GCTCTTC 1 cut(s) 1625
BspTI CTTAAG 1 cut(s) 2564
BsrDI GCAATG 2 cut(s) 375, 661
BsrFI RCCGGY 1 cut(s) 1707
BsrGI TGTACA 1 cut(s) 1803
BssAI RCCGGY 1 cut(s) 1707
BssECI CCNNGG 7 cut(s) 132, 230, 343, 556, 644, 757, 2350
BssNAI GTATAC 3 cut(s) 356, 1145, 1886
BssNI GRCGYC 1 cut(s) 2413
BssSI CACGAG 1 cut(s) 1734
BssT1I CCWWGG 4 cut(s) 132, 230, 644, 757
Bst1107I GTATAC 3 cut(s) 356, 1145, 1886
Bst2BI CACGAG 1 cut(s) 1734
Bst2UI CCWGG 6 cut(s) 557, 807, 1053, 1592, 1905, 2060
Bst4CI ACNGT 6 cut(s) 327, 879, 1285, 1890, 2036, 2159
Bst6I CTCTTC 2 cut(s) 1428, 1625
BstACI GRCGYC 1 cut(s) 2413
BstAFI CTTAAG 1 cut(s) 2564
BstAUI TGTACA 1 cut(s) 1803
BstBAI YACGTR 2 cut(s) 1295, 1670
BstC8I GCNNGC 4 cut(s) 366, 710, 1628, 1815
BstDEI CTNAG 9 cut(s) 63, 251, 371, 740, 1496, 1544, 1844, 1959, 2322
BstF5I GGATG 7 cut(s) 645, 856, 943, 1137, 1149, 2194, 2218
BstFNI CGCG 1 cut(s) 1463
BstH2I RGCGCY 2 cut(s) 1639, 2533
BstHHI GCGC 3 cut(s) 1463, 1638, 2532
BstMAI GTCTC 3 cut(s) 1540, 1985, 2306
BstMWI GCNNNNNNNGC 9 cut(s) 412, 847, 940, 1036, 1081, 1486, 1492, 1527, 1642
BstNI CCWGG 6 cut(s) 557, 807, 1053, 1592, 1905, 2060
BstNSI RCATGY 2 cut(s) 2303, 2360
BstSFI CTRYAG 2 cut(s) 1076, 1327
BstSLI GKGCMC 1 cut(s) 350
BstUI CGCG 1 cut(s) 1463
BstV2I GAAGAC 1 cut(s) 1448
BstX2I RGATCY 1 cut(s) 2135
BstXI CCANNNNNNTGG 3 cut(s) 563, 1295, 2387
BstYI RGATCY 1 cut(s) 2135
BstZ17I GTATAC 3 cut(s) 356, 1145, 1886
BsuI GTATCC 1 cut(s) 2140
BsuRI GGCC 5 cut(s) 723, 746, 756, 1480, 2058
BtsCI GGATG 7 cut(s) 645, 856, 943, 1137, 1149, 2194, 2218
BtsI GCAGTG 2 cut(s) 688, 1312
BtsIMutI CAGTG 8 cut(s) 74, 164, 688, 860, 911, 1312, 1861, 2141
BveI ACCTGC 1 cut(s) 1334
Cac8I GCNNGC 4 cut(s) 366, 710, 1628, 1815
CaiI CAGNNNCTG 3 cut(s) 421, 454, 838
CfoI GCGC 3 cut(s) 1463, 1638, 2532
Cfr10I RCCGGY 1 cut(s) 1707
Cfr13I GGNCC 5 cut(s) 609, 722, 744, 1468, 1588
Cfr9I CCCGGG 2 cut(s) 343, 2350
CpoI CGGWCCG 1 cut(s) 1468
CseI GACGC 2 cut(s) 460, 2402
Csp6I GTAC 4 cut(s) 94, 1687, 1711, 1804
CspAI ACCGGT 1 cut(s) 1707
CspCI CAANNNNNGTGG 2 cut(s) 2476, 2511
CspI CGGWCCG 1 cut(s) 1468
CviQI GTAC 4 cut(s) 94, 1687, 1711, 1804
DdeI CTNAG 9 cut(s) 63, 251, 371, 740, 1496, 1544, 1844, 1959, 2322
DraI TTTAAA 1 cut(s) 699
DriI GACNNNNNGTC 1 cut(s) 203
Eam1104I CTCTTC 2 cut(s) 1428, 1625
Eam1105I GACNNNNNGTC 1 cut(s) 203
EarI CTCTTC 2 cut(s) 1428, 1625
EciI GGCGGA 2 cut(s) 1481, 1589
Eco130I CCWWGG 4 cut(s) 132, 230, 644, 757
Eco47I GGWCC 3 cut(s) 609, 1468, 1588
Eco47III AGCGCT 1 cut(s) 1637
Eco57I CTGAAG 5 cut(s) 133, 228, 1606, 1913, 2483
Eco72I CACGTG 1 cut(s) 1670
Eco88I CYCGRG 3 cut(s) 343, 1718, 2350
EcoO109I RGGNCCY 2 cut(s) 609, 1588
EcoRII CCWGG 6 cut(s) 555, 805, 1051, 1590, 1903, 2058
EcoT14I CCWWGG 4 cut(s) 132, 230, 644, 757
EcoT22I ATGCAT 3 cut(s) 858, 945, 1098
ErhI CCWWGG 4 cut(s) 132, 230, 644, 757
FaqI GGGAC 7 cut(s) 240, 573, 1596, 1709, 1817, 2333, 2367
FbaI TGATCA 1 cut(s) 733
FblI GTMKAC 3 cut(s) 355, 1144, 1885
FokI GGATG 7 cut(s) 652, 843, 930, 1136, 1144, 2201, 2225
FspBI CTAG 4 cut(s) 105, 171, 816, 2394
GlaI GCGC 3 cut(s) 1462, 1637, 2531
GsuI CTGGAG 5 cut(s) 315, 1613, 1652, 1667, 1926
HaeII RGCGCY 2 cut(s) 1639, 2533
HaeIII GGCC 5 cut(s) 723, 746, 756, 1480, 2058
HgaI GACGC 2 cut(s) 460, 2402
HhaI GCGC 3 cut(s) 1463, 1638, 2532
Hin1I GRCGYC 1 cut(s) 2413
Hin6I GCGC 3 cut(s) 1461, 1636, 2530
HinP1I GCGC 3 cut(s) 1461, 1636, 2530
HincII GTYRAC 1 cut(s) 2422
HindII GTYRAC 1 cut(s) 2422
HinfI GANTC 7 cut(s) 418, 552, 959, 1436, 2004, 2216, 2418
HphI GGTGA 7 cut(s) 231, 867, 1005, 1056, 1107, 1707, 2218
Hpy166II GTNNAC 7 cut(s) 356, 790, 1145, 1288, 1806, 1886, 2422
Hpy188III TCNNGA 6 cut(s) 246, 294, 501, 1430, 1751, 2045
Hpy8I GTNNAC 7 cut(s) 356, 790, 1145, 1288, 1806, 1886, 2422
Hpy99I CGWCG 2 cut(s) 1476, 2418
HpyAV CCTTC 4 cut(s) 83, 222, 252, 746
HpyCH4III ACNGT 6 cut(s) 327, 879, 1285, 1890, 2036, 2159
HpyCH4IV ACGT 3 cut(s) 1294, 1669, 2509
HpyF10VI GCNNNNNNNGC 9 cut(s) 412, 847, 940, 1036, 1081, 1486, 1492, 1527, 1642
HpyF3I CTNAG 9 cut(s) 63, 251, 371, 740, 1496, 1544, 1844, 1959, 2322
HpySE526I ACGT 3 cut(s) 1294, 1669, 2509
Hsp92I GRCGYC 1 cut(s) 2413
HspAI GCGC 3 cut(s) 1461, 1636, 2530
Ksp22I TGATCA 1 cut(s) 733
LguI GCTCTTC 1 cut(s) 1625
LmnI GCTCC 5 cut(s) 1302, 1594, 1633, 1686, 2458
MaeI CTAG 4 cut(s) 105, 171, 816, 2394
MaeII ACGT 3 cut(s) 1294, 1669, 2509
MaeIII GTNAC 6 cut(s) 514, 811, 1394, 1670, 1954, 2224
MfeI CAATTG 2 cut(s) 845, 2538
MflI RGATCY 1 cut(s) 2135
MhlI GDGCHC 1 cut(s) 350
MlyI GAGTC 3 cut(s) 561, 1445, 2427
MmeI TCCRAC 4 cut(s) 1147, 1280, 1581, 2511
Mph1103I ATGCAT 3 cut(s) 858, 945, 1098
MroXI GAANNNNTTC 3 cut(s) 287, 1926, 1971
MseI TTAA 5 cut(s) 669, 698, 824, 2304, 2565
MslI CAYNNNNRTG 5 cut(s) 77, 263, 1293, 2211, 2385
MspA1I CMGCKG 2 cut(s) 838, 1129
MspCI CTTAAG 1 cut(s) 2564
MunI CAATTG 2 cut(s) 845, 2538
Mva1269I GAATGC 1 cut(s) 40
MvaI CCWGG 6 cut(s) 557, 807, 1053, 1592, 1905, 2060
MvnI CGCG 1 cut(s) 1463
MwoI GCNNNNNNNGC 9 cut(s) 412, 847, 940, 1036, 1081, 1486, 1492, 1527, 1642
NciI CCSGG 7 cut(s) 344, 345, 725, 1140, 2123, 2351, 2352
NlaIV GGNNCC 2 cut(s) 1312, 2137
NmuCI GTSAC 4 cut(s) 811, 1394, 1670, 2224
NsiI ATGCAT 3 cut(s) 858, 945, 1098
NspI RCATGY 2 cut(s) 2303, 2360
OliI CACNNNNGTG 1 cut(s) 1293
PciI ACATGT 1 cut(s) 2299
PciSI GCTCTTC 1 cut(s) 1625
PcsI WCGNNNNNNNCGW 3 cut(s) 1518, 1741, 2284
PctI GAATGC 1 cut(s) 40
PdmI GAANNNNTTC 3 cut(s) 287, 1926, 1971
PfeI GAWTC 4 cut(s) 418, 959, 2004, 2216
PflFI GACNNNGTC 1 cut(s) 252
PfoI TCCNGGA 2 cut(s) 1903, 2121
PinAI ACCGGT 1 cut(s) 1707
PleI GAGTC 3 cut(s) 560, 1444, 2426
PmaCI CACGTG 1 cut(s) 1670
PmlI CACGTG 1 cut(s) 1670
PpsI GAGTC 3 cut(s) 560, 1444, 2426
Ppu21I YACGTR 2 cut(s) 1295, 1670
PpuMI RGGWCCY 2 cut(s) 609, 1588
PscI ACATGT 1 cut(s) 2299
Psp5II RGGWCCY 2 cut(s) 609, 1588
Psp6I CCWGG 6 cut(s) 555, 805, 1051, 1590, 1903, 2058
PspCI CACGTG 1 cut(s) 1670
PspGI CCWGG 6 cut(s) 555, 805, 1051, 1590, 1903, 2058
PspN4I GGNNCC 2 cut(s) 1312, 2137
PspPI GGNCC 5 cut(s) 609, 722, 744, 1468, 1588
PspPPI RGGWCCY 2 cut(s) 609, 1588
PsrI GAACNNNNNNTAC 2 cut(s) 876, 908
PstI CTGCAG 2 cut(s) 1080, 1331
PstNI CAGNNNCTG 3 cut(s) 421, 454, 838
PsuI RGATCY 1 cut(s) 2135
PsyI GACNNNGTC 1 cut(s) 252
PvuII CAGCTG 1 cut(s) 838
RsaI GTAC 4 cut(s) 95, 1688, 1712, 1805
RsaNI GTAC 4 cut(s) 94, 1687, 1711, 1804
RseI CAYNNNNRTG 5 cut(s) 77, 263, 1293, 2211, 2385
Rsr2I CGGWCCG 1 cut(s) 1468
RsrII CGGWCCG 1 cut(s) 1468
SapI GCTCTTC 1 cut(s) 1625
SaqAI TTAA 5 cut(s) 669, 698, 824, 2304, 2565
Sau96I GGNCC 5 cut(s) 609, 722, 744, 1468, 1588
SchI GAGTC 3 cut(s) 561, 1445, 2427
SduI GDGCHC 1 cut(s) 350
SfcI CTRYAG 2 cut(s) 1076, 1327
SinI GGWCC 3 cut(s) 609, 1468, 1588
SmaI CCCGGG 2 cut(s) 345, 2352
SmiMI CAYNNNNRTG 5 cut(s) 77, 263, 1293, 2211, 2385
SmlI CTYRAG 4 cut(s) 604, 769, 1676, 2564
SmoI CTYRAG 4 cut(s) 604, 769, 1676, 2564
SpeI ACTAGT 1 cut(s) 815
SspMI CTAG 4 cut(s) 105, 171, 816, 2394
StyI CCWWGG 4 cut(s) 132, 230, 644, 757
TaaI ACNGT 6 cut(s) 327, 879, 1285, 1890, 2036, 2159
TaiI ACGT 3 cut(s) 1297, 1672, 2512
TaqI TCGA 9 cut(s) 196, 1280, 1439, 1535, 1750, 2278, 2287, 2383, 2416
TaqII GACCGA 2 cut(s) 1485, 2437
TatI WGTACW 2 cut(s) 1686, 1803
TauI GCSGC 5 cut(s) 406, 749, 1466, 1489, 2546
TfiI GAWTC 4 cut(s) 418, 959, 2004, 2216
Tru1I TTAA 5 cut(s) 669, 698, 824, 2304, 2565
Tru9I TTAA 5 cut(s) 669, 698, 824, 2304, 2565
TscAI CASTG 8 cut(s) 81, 164, 688, 867, 918, 1312, 1861, 2148
TseFI GTSAC 4 cut(s) 811, 1394, 1670, 2224
Tsp45I GTSAC 4 cut(s) 811, 1394, 1670, 2224
TspDTI ATGAA 9 cut(s) 17, 95, 475, 1224, 1339, 1346, 2039, 2195, 2357
TspGWI ACGGA 5 cut(s) 638, 652, 1469, 1489, 1527
TspMI CCCGGG 2 cut(s) 343, 2350
TspRI CASTG 8 cut(s) 81, 164, 688, 867, 918, 1312, 1861, 2148
Tth111I GACNNNGTC 1 cut(s) 252
Vha464I CTTAAG 1 cut(s) 2564
VpaK11BI GGWCC 3 cut(s) 609, 1468, 1588
XapI RAATTY 5 cut(s) 568, 799, 1379, 1969, 1996
XceI RCATGY 2 cut(s) 2303, 2360
XmaI CCCGGG 2 cut(s) 343, 2350
XmiI GTMKAC 3 cut(s) 355, 1144, 1885
XmnI GAANNNNTTC 3 cut(s) 287, 1926, 1971
XspI CTAG 4 cut(s) 105, 171, 816, 2394
Zsp2I ATGCAT 3 cut(s) 858, 945, 1098
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.