pycom16g03270

Protein of unknown function (DUF1218)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0007471
Physical Location & Seq
Reverse (-)
2044583 .. 2045983
1401 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 684 bp
ATGAGAGAAATCGAAAATCGAATGATAGTTCAAGGAGCAAATCGCAGGTTACTCAAACTCGAAAGAGAGACAACAATGGCAGTCTCGATACCCATTGTCGCCATCGTCACCTCCCTCCACCTCATCGCCTTCATCTTCGCCGTCGGCGCTGAACGGCGCCGTAGCACGGCGAAGATAGTGCCCGATGAGTACGACGAGCGTACCTACTGCGTGTACGGCACGGACGCCTCCACGGTGTACGGATTAGCGGCGTGTGGGCTGCTTCTGGTCAGCCAGGCGGTGCTTAACGGCGTCACCAGGTGTCTCTGTTGCGGCAAAGGTCTAGTCACTGGCTCGTCCACCACTTGGACCGTCTTCTTCTTCGTCTTCTCCTGGACAACCTTTTTGGGAGCGGAGGCGTGCTTGTTGGCTGGGTCGGCAAAGAATGCATACCACACCAAGTACAGGTCGATCTTTAATGCAGATGACTTGTCCTGTGCTACTCTACGCAAAGGGGTGTTTGCCGCCAGCGCTGCTCTTACGCTGTTGTCACTGGCAGGGTCAAGTCTTTACTACTGGGCACATTCCAAAGCTGATACTGGGGGATGGGAGAAGCACCGAAATGAAGGCCTTGACATGAGCAGTTCTAACTACGGGCAGCATGAGCAGCAGCAACAACAAGCCAGTGCATTTCAGAAGGTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1218

Protein Analysis

228

Amino Acids

24.85

Weight (kDa)

8.67

Isoelectric Point (pI)

30.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1218 PF06749 84 - 178 2.2e-25 Protein of unknown function (DUF1218)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 36
AccB1I GGYRCC 1 cut(s) 156
AccB7I CCANNNNNTGG 1 cut(s) 345
AccBSI CCGCTC 1 cut(s) 392
AciI CCGC 5 cut(s) 248, 278, 312, 392, 504
AcyI GRCGYC 3 cut(s) 157, 225, 291
AdeI CACNNNGTG 1 cut(s) 300
AfaI GTAC 5 cut(s) 191, 202, 215, 239, 443
AfeI AGCGCT 1 cut(s) 511
AfiI CCNNNNNNNGG 3 cut(s) 166, 345, 444
AgsI TTSAA 1 cut(s) 32
AjnI CCWGG 3 cut(s) 273, 296, 371
AluBI AGCT 1 cut(s) 572
AluI AGCT 1 cut(s) 572
Alw26I GTCTC 3 cut(s) 62, 88, 308
Aor51HI AGCGCT 1 cut(s) 511
AoxI GGCC 1 cut(s) 607
ApeKI GCWGC 5 cut(s) 259, 512, 637, 646, 649
ArsI GACNNNNNNTTYG 4 cut(s) 309, 341, 367, 399
AspLEI GCGC 3 cut(s) 149, 159, 512
AspS9I GGNCC 1 cut(s) 348
AsuHPI GGTGA 2 cut(s) 100, 286
AvaII GGWCC 1 cut(s) 348
BaeGI GKGCMC 2 cut(s) 183, 562
BaeI ACNNNNGTAYC 2 cut(s) 184, 217
BanI GGYRCC 1 cut(s) 156
BbsI GAAGAC 2 cut(s) 346, 358
BbvI GCAGC 5 cut(s) 246, 499, 649, 658, 661
BccI CCATC 2 cut(s) 110, 579
BceAI ACGGC 6 cut(s) 125, 144, 170, 183, 232, 304
BcgI CGANNNNNNTGC 2 cut(s) 160, 194
BciT130I CCWGG 3 cut(s) 275, 298, 373
BcoDI GTCTC 3 cut(s) 62, 88, 308
BfaI CTAG 1 cut(s) 323
BfoI RGCGCY 3 cut(s) 150, 160, 513
BfuAI ACCTGC 1 cut(s) 36
BisI GCNGC 8 cut(s) 249, 260, 313, 504, 513, 638, 647, 650
BlsI GCNGC 8 cut(s) 250, 261, 314, 505, 514, 639, 648, 651
Bme1390I CCNGG 3 cut(s) 275, 298, 373
Bme18I GGWCC 1 cut(s) 348
BmgT120I GGNCC 1 cut(s) 348
BmiI GGNNCC 1 cut(s) 158
BmrFI CCNGG 3 cut(s) 275, 298, 373
BmrI ACTGGG 2 cut(s) 565, 588
BmuI ACTGGG 2 cut(s) 565, 588
BpiI GAAGAC 2 cut(s) 346, 358
BsaHI GRCGYC 3 cut(s) 157, 225, 291
BsaJI CCNNGG 1 cut(s) 231
Bsc4I CCNNNNNNNGG 3 cut(s) 166, 345, 444
Bse1I ACTGG 5 cut(s) 334, 537, 560, 583, 663
BseBI CCWGG 3 cut(s) 275, 298, 373
BseDI CCNNGG 1 cut(s) 231
BseGI GGATG 1 cut(s) 590
BseLI CCNNNNNNNGG 3 cut(s) 166, 345, 444
BseNI ACTGG 5 cut(s) 334, 537, 560, 583, 663
BseSI GKGCMC 2 cut(s) 183, 562
BseXI GCAGC 5 cut(s) 246, 499, 649, 658, 661
BseYI CCCAGC 1 cut(s) 410
BshFI GGCC 1 cut(s) 609
BshNI GGYRCC 1 cut(s) 156
BslI CCNNNNNNNGG 3 cut(s) 166, 345, 444
BsmAI GTCTC 3 cut(s) 62, 88, 308
BsmI GAATGC 1 cut(s) 430
BsnI GGCC 1 cut(s) 609
Bsp1286I GDGCHC 2 cut(s) 183, 562
Bsp143I GATC 1 cut(s) 450
BspACI CCGC 5 cut(s) 248, 278, 312, 392, 504
BspANI GGCC 1 cut(s) 609
BspLI GGNNCC 1 cut(s) 158
BspMI ACCTGC 1 cut(s) 36
BspT107I GGYRCC 1 cut(s) 156
BsrBI CCGCTC 1 cut(s) 392
BsrI ACTGG 5 cut(s) 334, 537, 560, 583, 663
BssECI CCNNGG 1 cut(s) 231
BssMI GATC 1 cut(s) 450
BssNI GRCGYC 3 cut(s) 157, 225, 291
Bst2UI CCWGG 3 cut(s) 275, 298, 373
Bst4CI ACNGT 2 cut(s) 235, 352
BstACI GRCGYC 3 cut(s) 157, 225, 291
BstAPI GCANNNNNTGC 1 cut(s) 425
BstC8I GCNNGC 2 cut(s) 400, 508
BstDSI CCRYGG 1 cut(s) 231
BstF5I GGATG 1 cut(s) 590
BstH2I RGCGCY 3 cut(s) 150, 160, 513
BstHHI GCGC 3 cut(s) 149, 159, 512
BstKTI GATC 1 cut(s) 453
BstMAI GTCTC 3 cut(s) 62, 88, 308
BstMBI GATC 1 cut(s) 450
BstMWI GCNNNNNNNGC 8 cut(s) 146, 216, 416, 425, 509, 512, 643, 646
BstNI CCWGG 3 cut(s) 275, 298, 373
BstSCI CCNGG 3 cut(s) 273, 296, 371
BstSLI GKGCMC 2 cut(s) 183, 562
BstV1I GCAGC 5 cut(s) 246, 499, 649, 658, 661
BstV2I GAAGAC 2 cut(s) 346, 358
BsuRI GGCC 1 cut(s) 609
BtgI CCRYGG 1 cut(s) 231
BtgZI GCGATG 1 cut(s) 109
BtsCI GGATG 1 cut(s) 590
BtsIMutI CAGTG 3 cut(s) 327, 530, 670
BveI ACCTGC 1 cut(s) 36
Cac8I GCNNGC 2 cut(s) 400, 508
CfoI GCGC 3 cut(s) 149, 159, 512
Cfr13I GGNCC 1 cut(s) 348
CseI GACGC 2 cut(s) 233, 280
CsiI ACCWGGT 1 cut(s) 296
Csp6I GTAC 5 cut(s) 190, 201, 214, 238, 442
CviAII CATG 2 cut(s) 616, 641
CviJI RGCY 7 cut(s) 259, 273, 333, 410, 572, 609, 662
CviKI_1 RGCY 7 cut(s) 259, 273, 333, 410, 572, 609, 662
CviQI GTAC 5 cut(s) 190, 201, 214, 238, 442
DinI GGCGCC 1 cut(s) 158
DpnI GATC 1 cut(s) 452
DpnII GATC 1 cut(s) 450
DraIII CACNNNGTG 1 cut(s) 300
Eco147I AGGCCT 1 cut(s) 609
Eco47I GGWCC 1 cut(s) 348
Eco47III AGCGCT 1 cut(s) 511
EcoRII CCWGG 3 cut(s) 273, 296, 371
EcoT22I ATGCAT 1 cut(s) 430
EgeI GGCGCC 1 cut(s) 158
EheI GGCGCC 1 cut(s) 158
FaeI CATG 2 cut(s) 619, 644
FaiI YATR 4 cut(s) 430, 617, 642, 682
FatI CATG 2 cut(s) 615, 640
Fnu4HI GCNGC 8 cut(s) 249, 260, 313, 504, 513, 638, 647, 650
FokI GGATG 1 cut(s) 597
Fsp4HI GCNGC 8 cut(s) 249, 260, 313, 504, 513, 638, 647, 650
FspBI CTAG 1 cut(s) 323
GlaI GCGC 3 cut(s) 148, 158, 511
GluI GCNGC 8 cut(s) 249, 260, 313, 504, 513, 638, 647, 650
GsaI CCCAGC 1 cut(s) 414
HaeII RGCGCY 3 cut(s) 150, 160, 513
HaeIII GGCC 1 cut(s) 609
HgaI GACGC 2 cut(s) 233, 280
HhaI GCGC 3 cut(s) 149, 159, 512
Hin1I GRCGYC 3 cut(s) 157, 225, 291
Hin1II CATG 2 cut(s) 619, 644
Hin6I GCGC 3 cut(s) 147, 157, 510
HinP1I GCGC 3 cut(s) 147, 157, 510
HphI GGTGA 2 cut(s) 100, 286
Hpy166II GTNNAC 3 cut(s) 214, 238, 339
Hpy188I TCNGA 1 cut(s) 675
Hpy188III TCNNGA 1 cut(s) 85
Hpy8I GTNNAC 3 cut(s) 214, 238, 339
Hpy99I CGWCG 2 cut(s) 146, 197
HpyAV CCTTC 3 cut(s) 139, 599, 670
HpyCH4III ACNGT 2 cut(s) 235, 352
HpyCH4V TGCA 3 cut(s) 428, 461, 668
HpyF10VI GCNNNNNNNGC 8 cut(s) 146, 216, 416, 425, 509, 512, 643, 646
Hsp92I GRCGYC 3 cut(s) 157, 225, 291
Hsp92II CATG 2 cut(s) 619, 644
HspAI GCGC 3 cut(s) 147, 157, 510
KasI GGCGCC 1 cut(s) 156
Kzo9I GATC 1 cut(s) 450
LmnI GCTCC 2 cut(s) 35, 389
Lsp1109I GCAGC 5 cut(s) 246, 499, 649, 658, 661
MabI ACCWGGT 1 cut(s) 296
MaeI CTAG 1 cut(s) 323
MaeIII GTNAC 5 cut(s) 48, 106, 292, 325, 528
MalI GATC 1 cut(s) 452
MbiI CCGCTC 1 cut(s) 392
MboI GATC 1 cut(s) 450
MboII GAAGA 6 cut(s) 127, 184, 346, 349, 352, 358
MhlI GDGCHC 2 cut(s) 183, 562
Mly113I GGCGCC 1 cut(s) 157
MnlI CCTC 5 cut(s) 121, 125, 131, 238, 388
Mph1103I ATGCAT 1 cut(s) 430
MseI TTAA 2 cut(s) 285, 456
MslI CAYNNNNRTG 1 cut(s) 600
MspR9I CCNGG 3 cut(s) 275, 298, 373
Mva1269I GAATGC 1 cut(s) 430
MvaI CCWGG 3 cut(s) 275, 298, 373
MwoI GCNNNNNNNGC 8 cut(s) 146, 216, 416, 425, 509, 512, 643, 646
NarI GGCGCC 1 cut(s) 157
NdeII GATC 1 cut(s) 450
NlaIII CATG 2 cut(s) 619, 644
NlaIV GGNNCC 1 cut(s) 158
NmuCI GTSAC 4 cut(s) 106, 292, 325, 528
NsiI ATGCAT 1 cut(s) 430
PceI AGGCCT 1 cut(s) 609
PctI GAATGC 1 cut(s) 430
PflMI CCANNNNNTGG 1 cut(s) 345
PfoI TCCNGGA 1 cut(s) 371
PkrI GCNGC 8 cut(s) 250, 261, 314, 505, 514, 639, 648, 651
PluTI GGCGCC 1 cut(s) 160
Psp6I CCWGG 3 cut(s) 273, 296, 371
PspFI CCCAGC 1 cut(s) 410
PspGI CCWGG 3 cut(s) 273, 296, 371
PspN4I GGNNCC 1 cut(s) 158
PspPI GGNCC 1 cut(s) 348
RsaI GTAC 5 cut(s) 191, 202, 215, 239, 443
RsaNI GTAC 5 cut(s) 190, 201, 214, 238, 442
RseI CAYNNNNRTG 1 cut(s) 600
SaqAI TTAA 2 cut(s) 285, 456
SatI GCNGC 8 cut(s) 249, 260, 313, 504, 513, 638, 647, 650
Sau3AI GATC 1 cut(s) 450
Sau96I GGNCC 1 cut(s) 348
ScrFI CCNGG 3 cut(s) 275, 298, 373
SduI GDGCHC 2 cut(s) 183, 562
SexAI ACCWGGT 1 cut(s) 296
SfoI GGCGCC 1 cut(s) 158
SinI GGWCC 1 cut(s) 348
SmiMI CAYNNNNRTG 1 cut(s) 600
SseBI AGGCCT 1 cut(s) 609
SsiI CCGC 5 cut(s) 248, 278, 312, 392, 504
SspDI GGCGCC 1 cut(s) 156
SspMI CTAG 1 cut(s) 323
StuI AGGCCT 1 cut(s) 609
StyD4I CCNGG 3 cut(s) 273, 296, 371
TaaI ACNGT 2 cut(s) 235, 352
TaqI TCGA 5 cut(s) 12, 19, 60, 86, 449
TatI WGTACW 1 cut(s) 441
TauI GCSGC 3 cut(s) 251, 315, 506
Tru1I TTAA 2 cut(s) 285, 456
Tru9I TTAA 2 cut(s) 285, 456
TscAI CASTG 3 cut(s) 334, 537, 670
TseFI GTSAC 4 cut(s) 106, 292, 325, 528
TseI GCWGC 5 cut(s) 259, 512, 637, 646, 649
Tsp45I GTSAC 4 cut(s) 106, 292, 325, 528
TspDTI ATGAA 2 cut(s) 121, 618
TspGWI ACGGA 2 cut(s) 236, 255
TspRI CASTG 3 cut(s) 334, 537, 670
Van91I CCANNNNNTGG 1 cut(s) 345
VpaK11BI GGWCC 1 cut(s) 348
XspI CTAG 1 cut(s) 323
Zsp2I ATGCAT 1 cut(s) 430
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.