pycom16g03780

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0008703
Physical Location & Seq
Forward (+)
2343654 .. 2344025
372 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 372 bp
ATGCTTAACGGAAAAGAAACTGAGGCAGAGAAGAGAAAGCAGGCGGACGGCGACGGCAACGTCTCAGGGAGGCAAAAACATAAGAAGGCGCGGGATGGTAACGAAGACGTCACGGCCGTGAAGGAGGAAGAGTTTGAGGAGTTCTTCGCTATCCTCGGGCGGATACGCGGGGCGGTCAGGCACTTCGAGAGTGCTAACGGCGGCGGCGTCCGTAACGAAACGGGCGGAGGATCCCGATTGAGGGCAACGCTCGAGAGTTACGAGGAAGCAAGCGGCGTCCAAGTGGGGCCCAAGAGGGAGAATTGTACGGTGGAGGAGAATTTGGGCTTGGATTTGAATGAGGATCCGAAACCGGAATGTGATCCAAGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

124

Amino Acids

13.43

Weight (kDa)

4.97

Isoelectric Point (pI)

51.95

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 59
AatII GACGTC 1 cut(s) 111
AccII CGCG 2 cut(s) 91, 168
AciI CCGC 9 cut(s) 44, 91, 160, 168, 173, 201, 204, 225, 273
AclWI GGATC 5 cut(s) 225, 238, 338, 351, 356
AcoI YGGCCR 1 cut(s) 114
AcsI RAATTY 1 cut(s) 319
AcyI GRCGYC 3 cut(s) 108, 207, 276
AfaI GTAC 1 cut(s) 307
AfiI CCNNNNNNNGG 2 cut(s) 240, 241
AgsI TTSAA 1 cut(s) 337
AjuI GAANNNNNNNTTGG 2 cut(s) 311, 343
AleI CACNNNNGTG 1 cut(s) 116
Alw26I GTCTC 1 cut(s) 67
AlwI GGATC 5 cut(s) 225, 238, 338, 351, 356
Ama87I CYCGRG 2 cut(s) 155, 251
AoxI GGCC 2 cut(s) 114, 287
ApaI GGGCCC 1 cut(s) 291
ApoI RAATTY 1 cut(s) 319
AspLEI GCGC 1 cut(s) 91
AspS9I GGNCC 2 cut(s) 287, 288
AvaI CYCGRG 2 cut(s) 155, 251
BaeGI GKGCMC 1 cut(s) 291
BamHI GGATCC 2 cut(s) 230, 343
BanII GRGCYC 1 cut(s) 291
BbsI GAAGAC 1 cut(s) 111
BccI CCATC 1 cut(s) 89
BceAI ACGGC 5 cut(s) 64, 70, 101, 129, 214
BciVI GTATCC 1 cut(s) 156
BcoDI GTCTC 1 cut(s) 67
BfuI GTATCC 1 cut(s) 156
BisI GCNGC 3 cut(s) 202, 205, 274
BlsI GCNGC 3 cut(s) 203, 206, 275
BmeT110I CYCGRG 2 cut(s) 155, 251
BmgT120I GGNCC 2 cut(s) 287, 288
BmiI GGNNCC 4 cut(s) 232, 288, 289, 345
BpiI GAAGAC 1 cut(s) 111
BsaHI GRCGYC 3 cut(s) 108, 207, 276
BsaJI CCNNGG 1 cut(s) 154
BsaWI WCCGGW 1 cut(s) 352
BsaXI ACNNNNNCTCC 2 cut(s) 116, 146
Bsc4I CCNNNNNNNGG 2 cut(s) 240, 241
BseDI CCNNGG 1 cut(s) 154
BseGI GGATG 1 cut(s) 100
BseLI CCNNNNNNNGG 2 cut(s) 240, 241
BseMII CTCAG 2 cut(s) 12, 78
BseRI GAGGAG 2 cut(s) 152, 329
BseSI GKGCMC 1 cut(s) 291
BseX3I CGGCCG 1 cut(s) 114
Bsh1236I CGCG 2 cut(s) 91, 168
Bsh1285I CGRYCG 1 cut(s) 117
BshFI GGCC 2 cut(s) 116, 289
BsiEI CGRYCG 1 cut(s) 117
BsiHKCI CYCGRG 2 cut(s) 155, 251
BsiSI CCGG 1 cut(s) 353
BslI CCNNNNNNNGG 2 cut(s) 240, 241
BsmAI GTCTC 1 cut(s) 67
BsmBI CGTCTC 1 cut(s) 67
BsnI GGCC 2 cut(s) 116, 289
BsoBI CYCGRG 2 cut(s) 155, 251
Bsp120I GGGCCC 1 cut(s) 287
Bsp1286I GDGCHC 1 cut(s) 291
Bsp143I GATC 3 cut(s) 230, 343, 361
BspACI CCGC 9 cut(s) 44, 91, 160, 168, 173, 201, 204, 225, 273
BspANI GGCC 2 cut(s) 116, 289
BspCNI CTCAG 2 cut(s) 13, 77
BspFNI CGCG 2 cut(s) 91, 168
BspLI GGNNCC 4 cut(s) 232, 288, 289, 345
BspPI GGATC 5 cut(s) 225, 238, 338, 351, 356
BssECI CCNNGG 1 cut(s) 154
BssMI GATC 3 cut(s) 230, 343, 361
BssNI GRCGYC 3 cut(s) 108, 207, 276
Bst4CI ACNGT 1 cut(s) 310
Bst6I CTCTTC 2 cut(s) 26, 123
BstACI GRCGYC 3 cut(s) 108, 207, 276
BstC8I GCNNGC 2 cut(s) 42, 271
BstDEI CTNAG 2 cut(s) 21, 64
BstF5I GGATG 1 cut(s) 100
BstFNI CGCG 2 cut(s) 91, 168
BstHHI GCGC 1 cut(s) 91
BstKTI GATC 3 cut(s) 233, 346, 364
BstMAI GTCTC 1 cut(s) 67
BstMBI GATC 3 cut(s) 230, 343, 361
BstMCI CGRYCG 1 cut(s) 117
BstSLI GKGCMC 1 cut(s) 291
BstUI CGCG 2 cut(s) 91, 168
BstV2I GAAGAC 1 cut(s) 111
BstX2I RGATCY 2 cut(s) 230, 343
BstYI RGATCY 2 cut(s) 230, 343
BstZI CGGCCG 1 cut(s) 114
BsuI GTATCC 1 cut(s) 156
BsuRI GGCC 2 cut(s) 116, 289
BtsCI GGATG 1 cut(s) 100
Cac8I GCNNGC 2 cut(s) 42, 271
CfoI GCGC 1 cut(s) 91
Cfr13I GGNCC 2 cut(s) 287, 288
CseI GACGC 2 cut(s) 196, 265
Csp6I GTAC 1 cut(s) 306
CviJI RGCY 3 cut(s) 116, 289, 327
CviKI_1 RGCY 3 cut(s) 116, 289, 327
CviQI GTAC 1 cut(s) 306
DdeI CTNAG 2 cut(s) 21, 64
DpnI GATC 3 cut(s) 232, 345, 363
DpnII GATC 3 cut(s) 230, 343, 361
DrdI GACNNNNNNGTC 1 cut(s) 59
DseDI GACNNNNNNGTC 1 cut(s) 59
EaeI YGGCCR 1 cut(s) 114
EagI CGGCCG 1 cut(s) 114
Eam1104I CTCTTC 2 cut(s) 26, 123
EarI CTCTTC 2 cut(s) 26, 123
EciI GGCGGA 3 cut(s) 59, 175, 240
EclXI CGGCCG 1 cut(s) 114
Eco24I GRGCYC 1 cut(s) 291
Eco52I CGGCCG 1 cut(s) 114
Eco88I CYCGRG 2 cut(s) 155, 251
EcoO109I RGGNCCY 1 cut(s) 287
EcoT38I GRGCYC 1 cut(s) 291
Esp3I CGTCTC 1 cut(s) 67
FaiI YATR 1 cut(s) 81
FauI CCCGC 2 cut(s) 84, 161
Fnu4HI GCNGC 3 cut(s) 202, 205, 274
FokI GGATG 1 cut(s) 107
FriOI GRGCYC 1 cut(s) 291
Fsp4HI GCNGC 3 cut(s) 202, 205, 274
GlaI GCGC 1 cut(s) 90
GluI GCNGC 3 cut(s) 202, 205, 274
HaeIII GGCC 2 cut(s) 116, 289
HapII CCGG 1 cut(s) 353
HgaI GACGC 2 cut(s) 196, 265
HhaI GCGC 1 cut(s) 91
Hin1I GRCGYC 3 cut(s) 108, 207, 276
Hin6I GCGC 1 cut(s) 89
HinP1I GCGC 1 cut(s) 89
HpaII CCGG 1 cut(s) 353
Hpy188I TCNGA 1 cut(s) 348
Hpy188III TCNNGA 3 cut(s) 187, 234, 253
Hpy99I CGWCG 1 cut(s) 56
HpyAV CCTTC 2 cut(s) 79, 115
HpyCH4III ACNGT 1 cut(s) 310
HpyCH4IV ACGT 2 cut(s) 60, 108
HpyF3I CTNAG 2 cut(s) 21, 64
HpySE526I ACGT 2 cut(s) 60, 108
Hsp92I GRCGYC 3 cut(s) 108, 207, 276
HspAI GCGC 1 cut(s) 89
Kzo9I GATC 3 cut(s) 230, 343, 361
LpnPI CCDG 4 cut(s) 26, 51, 163, 366
MaeII ACGT 2 cut(s) 60, 108
MaeIII GTNAC 4 cut(s) 98, 109, 212, 257
MalI GATC 3 cut(s) 232, 345, 363
MboI GATC 3 cut(s) 230, 343, 361
MboII GAAGA 4 cut(s) 43, 116, 136, 140
MflI RGATCY 2 cut(s) 230, 343
MhlI GDGCHC 1 cut(s) 291
MluCI AATT 2 cut(s) 301, 319
MseI TTAA 1 cut(s) 6
MslI CAYNNNNRTG 1 cut(s) 116
MspI CCGG 1 cut(s) 353
MvnI CGCG 2 cut(s) 91, 168
NdeII GATC 3 cut(s) 230, 343, 361
NlaIV GGNNCC 4 cut(s) 232, 288, 289, 345
NmuCI GTSAC 1 cut(s) 109
OliI CACNNNNGTG 1 cut(s) 116
PaeR7I CTCGAG 1 cut(s) 251
PcsI WCGNNNNNNNCGW 3 cut(s) 114, 204, 258
PkrI GCNGC 3 cut(s) 203, 206, 275
PspN4I GGNNCC 4 cut(s) 232, 288, 289, 345
PspOMI GGGCCC 1 cut(s) 287
PspPI GGNCC 2 cut(s) 287, 288
PsuI RGATCY 2 cut(s) 230, 343
RsaI GTAC 1 cut(s) 307
RsaNI GTAC 1 cut(s) 306
RseI CAYNNNNRTG 1 cut(s) 116
SaqAI TTAA 1 cut(s) 6
SatI GCNGC 3 cut(s) 202, 205, 274
Sau3AI GATC 3 cut(s) 230, 343, 361
Sau96I GGNCC 2 cut(s) 287, 288
SduI GDGCHC 1 cut(s) 291
SetI ASST 2 cut(s) 63, 111
Sfr274I CTCGAG 1 cut(s) 251
SlaI CTCGAG 1 cut(s) 251
SmiMI CAYNNNNRTG 1 cut(s) 116
SmlI CTYRAG 1 cut(s) 251
SmoI CTYRAG 1 cut(s) 251
Sse9I AATT 2 cut(s) 301, 319
SsiI CCGC 9 cut(s) 44, 91, 160, 168, 173, 201, 204, 225, 273
TaaI ACNGT 1 cut(s) 310
TaiI ACGT 2 cut(s) 63, 111
TaqI TCGA 2 cut(s) 186, 252
TasI AATT 2 cut(s) 301, 319
TauI GCSGC 3 cut(s) 204, 207, 276
Tru1I TTAA 1 cut(s) 6
Tru9I TTAA 1 cut(s) 6
TseFI GTSAC 1 cut(s) 109
Tsp45I GTSAC 1 cut(s) 109
TspGWI ACGGA 2 cut(s) 24, 200
XapI RAATTY 1 cut(s) 319
XhoI CTCGAG 1 cut(s) 251
ZraI GACGTC 1 cut(s) 109
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.