pycom16g04620

Protein PHYLLO, chloroplastic-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0006265
Physical Location & Seq
Forward (+)
2953737 .. 2961532
7796 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 4227 bp
ATGTTGTGGCTAACATCACGACGCTGGTATCCCCCTGTCCTGAAAGAGACTCCGTTTTCCTGCCAGTTTTGTATCAAGCTTTCACCTACCATTGCAGTTTCTAGCAACATGTTGGACCATACCAATAAAATGTGCTACTCAGTTGAAGATTGGGCCAACATCAATACTGTTTGGGCATCACTTATAATTGAAGAATGTTCTCGACTTGGTTTGACGTATTTTTGTGTAGCTCCAGGATCAAGATCATCCCCTCTTGCTGTTGCTGCATCCTCTCATCCCCTTATTACATGTATGGTATGTTATGACGAACGTTCACTTGCATTTCATGCTGTTGGTTATGCAAGAGGATCTCAGAAGCCAGCTGTTGTCATAACGTCATCTGGAACTGCAGTTTCAAACCTTCTTCCTGCTGTTGTCGAGGCTAGTCAAGATTTTGTACCACTCCTATTGCTCACAGCTGACCGTCCTGCAGAATTGCATGATGCTGGAGCAAACCAAGCTATCAATCAGGTAAACCATTTTGGTTCTTTTGTGAGGTTTTTCTTCAGCCTTCCAGCAGCCACCGATCATATTTCAGCAAGGATGGTACTTACAACACTTGACTCTGCTGTACATTGGGCAACCTCGTCACCGTGTGGCCCTGTTCATATTAACTGTCCATTCAGGGAGCCACGGGAAAATAGCCCTAGCAAATGGATGACGAGTTGTTTGAAAGGATTAGACTTTTGGATGTCTAGCACCGAGCCATTTACTAACTATATTAAAGTACAGAGTGCTCACACGTATGATGATGGTTGTGGGCAAATGTCAGAGGTTCTAAATCTAATTAGAGGTACTAACAAAGGGATTCTAGTAATTGGTGCAATCCATTCAGAAGATGAGATGTGGGCAGTTCTTCTCTTGGTTAAACACCTTCAATGGCCTGTTGTAGCTGACATCCTGTCAGGATTACGATTGAGAAAGCTGCTGACTTCTTTTCCTGAAATTGGAGATGATTTGTTATTCGTTGATCATCTTGACCATGCTCTACTTTCAGATTCCGTGCGTAGTTGGATAAATTTTGATCTGATTATTCAGATTGGGAGTAGAATTACAAGCAAGCGTGTTGCTAAAATGCTAGAGGACTGCTTTCCCTGCTCATATATCTTGGTTGACGAGCATCCATTTCGTCATGATCCCTCTCATATTGTAACCCATAGAATCCAAAGTAGCATTGTTGAATTTTCCGATTGTTTATGTAAAGCTGGGCTTCCCTGTATGAGCAAAGAATGGAACACTTATCTTCAAACGTTAAATGTGATGGTTTCGAGGGAGTTGTCATTTCAAATTTATGCCAGAGACTTCTTGACTGAACCTCAAGTTGCAAGCGTGATATCCAAGGCACTTTCAGCTGAGTCTGCTCTTTTCATTGGGAACAGTATGGCGATACGTGATGCAGATATGTACGGATGTGGTTGGTCAGGTTGTAGTCATAATATTGCATCCATGATTTCAAAATTACAACTACCCTGTCATATGATTCGGGTGGCTGGGAATAGGGGTGCCAGTGGTATTGATGGGTTACTCAGCACAGCTGTTGGCTTTGCCGTTGGATGCAATAAACGGGTGCTTTGCGTGATTGGGGATGTTTCTTTCCTCCACGATACAAATGGCTTGGCAATTGTGAACCAGAGGACATTGCGGAAACCAATGACGATAGTTGTGATCAATAATCATGGTGGTGCAATATTCAGTCTCCTTCCTATAGCGGATAGAGTTGAGCCAAGCATACTAAATCAATACTTCTACACCTCTCACAATGTTTCAATCCACAACCTGTGTGTGGCACATGGTGTGATGCATCTACATGTGAAGACAAAGGTGGAGCTGGAAGATGCATTATTGACATCTCAAGATAAAGAGGTGGATTGTGTCATTGAAGTTGAGGGCTGCATAGATGCCAATGCCACCTTTCACAGTACTTTAAGAAAGTTTGCGTGCCAAGCAGCAGATCATGCGTTGAGCCTCTCCTCAAAGATTTCTGTTCAAGACTCACCAGCAGATGGCACTTCACTTTATAGAGTCCATAGAATGGAGTATTCAGTATTTAGTGTACCACTCTGTGCTCCTCCTACTATGGTTTCTGTTGATGATAATGAAACCAGCTTCTACAGAGAAGGGTTTATTTTAACTTTGTATCTTGAGGATGGAAGCATTGGGTTTGGCGAGGTTTCACCTCTCGATATCCACAGAGAAAGCCTGCTAGATGTAGAAGAGCAACTGAGGTTACTTGTTCATATGATGGAAGGAGCTAAGATTAGTTGCCTCCTTCCTCTGCTAAAGGGCTCTTTTTCTTCTTGGATATGGACCAACCTGGGAATACTGCCATGCACGCTCTTGCCTAGTGTTAGATGTGGTTTGGAAATGGCAATTCTCAATGCATTAGCGACAAGGCAAGGTTCCAACTTATTAGGCCTACTCCATCCCTTGAAAGCTGAAGGAGGTATATCTGAAAGGCCGATGACAGTTCAGATTTGTGCCCTAGTTGATTCCAATAGAACGCCCACACAAGTAGCTGATGTTGTAGCAGCACTTGTTGAAGAAGGATTTACTGCAGTAAAACTGAAGGTAGCACGTCAGGGGAGTCCCTTGCACGATGCTGCAGTTATACAGGCAGTAAGAAAGAAAGTTGGGTACCAGATTCAAGTTCGTGCAGATGCCAATCGGAACTGGACATATAAAGAAGCTATTCAGTTTGGTTCCTTGGTAAAGGATTGTGATCTGCAGTATATTGAGGAACCTGTTCAAAACGAAGGTGATATTGTCAAGTTCTGTGAAGAAAGCGGGTTACCCGTGGCACTGGATGAAACTATTGACAGTATTGGAGAACATCCACTTGACAAGCTAGTGAAGTACACCCATCCAGGAATAGTAGCTATTGTTATTAAACCTAGTGTTGTTGGTGGCTTTGAAAATGCAGCAATAATTGCTCAGTGGGCTCAGCAGCACCAGAAAATGGCTATTGTTAGTGCTGCATTTGAAAGTGGCCTAGGTTTGTCAGCATATATTCAGTTTTGCTGTTATCTCAACCTGAAAAATTCAGAAATCTGTGAAATGATGAATTATGAATTGGCCCCGTCAATAGCCCATGGTTTAGGAACATACCGCTGGGTAAAAGAAGATGTAACAACCACTCCTCTCAAGATAGGTTGTAATCCAGTCAGTGGCTTCGTAGAAGCATCTGTTGCTGATGCTGATCAAGTTCTTCAAAAATTTCAAATGAATGGCAATGTAGTTCATAGAAATTTTACTGGAGAGCAAGTCCGCGTATTCCAGCTTACTGTAGATTCAAAGGCATTCTCTTATTCCATCATAGTCCACGAGATTGGAGAAAGATACAATGAAAATGTTTTTGTATTTCTTCATGGGTTTCTTGGAACTGGTGAAGATTGGATTGCTATGATGAAGGCGATTTCAGGATGTGCAAGATGCATTTCGATTGACCTTCCTGGTCATGGTGGAACAAAGATACAGAACCATGGTGATAACGATGCTGTACAGGATTCAGGTTTGTCAATTGAGGTTGTTGCCGATCTTTTATGTGAAGTGATTAAACAGATTACGCCTGGAAAGGTTACTATTGTGGGTTACTCTATGGGAGCAAGGATTGCACTGTACATGGCTTTGAGGTGTACCGACAAAGTCAATGGAGCTGTTGTAATATCTGGAAGCCCTGGGTTAAAAGATGAAGTGGATAGAAAAATTCGTAGAGCTAAGGATGACTCTAGAGCTCGCTTCCTTATTGATCACGGCTTAGAGCTCTTCCTAGACACCTGGTATTCTGGAGAGCTGTGGAACAGCTTAAGAGTCCATCCCCGATTTCGTCAGATAGTTTCCAGCCGCCAGCATCATGAAGATGTGCAGAGTCTTGCAAAAGTGCTTTCCGGTTTAAGTATTGGGAGGCAGCCGCCATTGTGGGAAGACTTGAAGCACTGCAAAACACCCCTTTTGCTAATTGTTGGAGAGGAGGATAAAAAGTTCAAGGCGATAGCTAAGGATATGTCCTGTGAAATTGCCGGTGGCACGGTGAGTGGAGACGGCCCTCCCCATGACATCTATGAGATAGTTGAGATTCCAGATTGCGGACATGCTGCTCATCTGGAGAACCCTCTCCCTGTAATCAGCACATTGAGACGATTTTTGGGGAGAATGAACTCGAACTTGATTCAAAACCAGAAGGCAATTTAG

Protein Analysis

1409

Amino Acids

155.02

Weight (kDa)

5.92

Isoelectric Point (pI)

37.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPP_enzyme_N PF02776 59 - 171 9.2e-21 Thiamine pyrophosphate enzyme, N-terminal TPP binding domain
TPP_enzyme_M_2 PF16582 236 - 470 1.2e-21 Middle domain of thiamine pyrophosphate
TPP_enzyme_C PF02775 499 - 641 2.7e-08 Thiamine pyrophosphate enzyme, C-terminal TPP binding domain
MR_MLE_C PF13378 851 - 1037 3.7e-26 Enolase C-terminal domain-like
Abhydrolase_1 PF00561 1129 - 1383 1.8e-21 alpha/beta hydrolase fold
Abhydrolase_6 PF12697 1131 - 1386 2.6e-21 Alpha/beta hydrolase family
Hydrolase_4 PF12146 1131 - 1338 9.9e-08 Serine aminopeptidase, S33
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 185
Acc65I GGTACC 1 cut(s) 2673
AccB1I GGYRCC 2 cut(s) 1541, 2673
AccB7I CCANNNNNTGG 4 cut(s) 2042, 2071, 2995, 3203
AccII CGCG 1 cut(s) 3306
AciI CCGC 8 cut(s) 1681, 1748, 2823, 3145, 3304, 3880, 3947, 4122
AclI AACGTT 2 cut(s) 310, 1289
AclWI GGATC 3 cut(s) 244, 355, 1169
AcsI RAATTY 7 cut(s) 1057, 1220, 1326, 3076, 3251, 3283, 3741
AcuI CTGAAG 3 cut(s) 529, 2496, 2624
AdeI CACNNNGTG 3 cut(s) 635, 1832, 2102
AfiI CCNNNNNNNGG 8 cut(s) 986, 1822, 2042, 2071, 2995, 3203, 3494, 4121
AflII CTTAAG 1 cut(s) 3841
AflIII ACRYGT 4 cut(s) 108, 287, 780, 1846
AhdI GACNNNNNGTC 1 cut(s) 940
AjiI CACGTC 1 cut(s) 2615
AjnI CCWGG 7 cut(s) 232, 2352, 2902, 3487, 3604, 3712, 3812
AjuI GAANNNNNNNTTGG 2 cut(s) 3092, 3124
AloI GAACNNNNNNTCC 2 cut(s) 4001, 4033
Alw21I GWGCWC 4 cut(s) 778, 2107, 3772, 3801
Alw26I GTCTC 5 cut(s) 41, 1332, 1739, 4068, 4165
AlwI GGATC 3 cut(s) 244, 355, 1169
AoxI GGCC 8 cut(s) 153, 637, 920, 2452, 2495, 3025, 3111, 4078
ApoI RAATTY 7 cut(s) 1057, 1220, 1326, 3076, 3251, 3283, 3741
ArsI GACNNNNNNTTYG 2 cut(s) 691, 723
Asp700I GAANNNNTTC 2 cut(s) 2727, 2781
Asp718I GGTACC 1 cut(s) 2673
AspA2I CCTAGG 1 cut(s) 3028
AspS9I GGNCC 6 cut(s) 115, 153, 638, 2346, 3112, 4079
AsuHPI GGTGA 8 cut(s) 75, 621, 2025, 2205, 2807, 3434, 3533, 4078
AvaII GGWCC 2 cut(s) 115, 2346
AvrII CCTAGG 1 cut(s) 3028
BaeGI GKGCMC 1 cut(s) 2521
BaeI ACNNNNGTAYC 2 cut(s) 11, 44
BanI GGYRCC 2 cut(s) 1541, 2673
BanII GRGCYC 4 cut(s) 2327, 2980, 3772, 3801
BauI CACGAG 1 cut(s) 3359
BbsI GAAGAC 2 cut(s) 1859, 3966
Bbv12I GWGCWC 4 cut(s) 778, 2107, 3772, 3801
BceAI ACGGC 3 cut(s) 1571, 3805, 4093
BcgI CGANNNNNNTGC 2 cut(s) 1148, 1182
BciT130I CCWGG 7 cut(s) 234, 2354, 2904, 3489, 3606, 3714, 3814
BciVI GTATCC 1 cut(s) 39
BclI TGATCA 4 cut(s) 1009, 1704, 3235, 3784
BcoDI GTCTC 5 cut(s) 41, 1332, 1739, 4068, 4165
BfmI CTRYAG 8 cut(s) 387, 468, 1743, 2149, 2592, 2640, 2762, 3321
BfrI CTTAAG 1 cut(s) 3841
BfuI GTATCC 1 cut(s) 39
BlnI CCTAGG 1 cut(s) 3028
BlpI GCTNAGC 1 cut(s) 2979
BmcAI AGTACT 1 cut(s) 1960
Bme1390I CCNGG 7 cut(s) 234, 2354, 2904, 3489, 3606, 3714, 3814
Bme18I GGWCC 2 cut(s) 115, 2346
BmeRI GACNNNNNGTC 1 cut(s) 940
BmgBI CACGTC 1 cut(s) 2615
BmgT120I GGNCC 6 cut(s) 115, 153, 638, 2346, 3112, 4079
BmiI GGNNCC 7 cut(s) 669, 1543, 2440, 2675, 2740, 2778, 3114
BmrFI CCNGG 7 cut(s) 234, 2354, 2904, 3489, 3606, 3714, 3814
BpiI GAAGAC 2 cut(s) 1859, 3966
BpmI CTGGAG 5 cut(s) 216, 507, 3312, 3843, 4160
Bpu10I CCTNAGC 2 cut(s) 3753, 4032
Bpu1102I GCTNAGC 1 cut(s) 2979
BpuEI CTTGAG 4 cut(s) 1341, 1875, 2201, 3164
BsaAI YACGTR 2 cut(s) 783, 1430
BsaBI GATNNNNATC 4 cut(s) 241, 2700, 2757, 3234
BsaWI WCCGGW 1 cut(s) 3923
BsaXI ACNNNNNCTCC 6 cut(s) 3157, 3187, 3285, 3315, 3681, 3711
Bsc4I CCNNNNNNNGG 8 cut(s) 986, 1822, 2042, 2071, 2995, 3203, 3494, 4121
Bse118I RCCGGY 1 cut(s) 4055
Bse1I ACTGG 7 cut(s) 64, 1545, 2714, 2844, 3197, 3295, 3424
Bse3DI GCAATG 3 cut(s) 90, 1676, 3274
Bse8I GATNNNNATC 4 cut(s) 241, 2700, 2757, 3234
BseBI CCWGG 7 cut(s) 234, 2354, 2904, 3489, 3606, 3714, 3814
BseJI GATNNNNATC 4 cut(s) 241, 2700, 2757, 3234
BseLI CCNNNNNNNGG 8 cut(s) 986, 1822, 2042, 2071, 2995, 3203, 3494, 4121
BseMI GCAATG 3 cut(s) 90, 1676, 3274
BseMII CTCAG 7 cut(s) 153, 365, 1383, 1579, 2252, 2984, 2993
BseNI ACTGG 7 cut(s) 64, 1545, 2714, 2844, 3197, 3295, 3424
BseRI GAGGAG 4 cut(s) 1999, 2097, 3165, 4019
BseSI GKGCMC 1 cut(s) 2521
BseYI CCCAGC 3 cut(s) 1244, 1529, 3147
BsgI GTGCAG 2 cut(s) 2712, 3920
Bsh1236I CGCG 1 cut(s) 3306
BshFI GGCC 8 cut(s) 155, 639, 922, 2454, 2497, 3027, 3113, 4080
BshNI GGYRCC 2 cut(s) 1541, 2673
BsiHKAI GWGCWC 4 cut(s) 778, 2107, 3772, 3801
BsiSI CCGG 2 cut(s) 3924, 4056
BslFI GGGAC 1 cut(s) 2610
BslI CCNNNNNNNGG 8 cut(s) 986, 1822, 2042, 2071, 2995, 3203, 3494, 4121
BsmAI GTCTC 5 cut(s) 41, 1332, 1739, 4068, 4165
BsmBI CGTCTC 2 cut(s) 4068, 4165
BsmFI GGGAC 1 cut(s) 2610
BsmI GAATGC 1 cut(s) 3335
BsnI GGCC 8 cut(s) 155, 639, 922, 2454, 2497, 3027, 3113, 4080
Bsp1286I GDGCHC 7 cut(s) 778, 2107, 2327, 2521, 2980, 3772, 3801
Bsp1407I TGTACA 3 cut(s) 610, 3535, 3654
Bsp1720I GCTNAGC 1 cut(s) 2979
Bsp19I CCATGG 2 cut(s) 3127, 3517
BspACI CCGC 8 cut(s) 1681, 1748, 2823, 3145, 3304, 3880, 3947, 4122
BspANI GGCC 8 cut(s) 155, 639, 922, 2454, 2497, 3027, 3113, 4080
BspCNI CTCAG 7 cut(s) 152, 364, 1384, 1578, 2253, 2983, 2992
BspFNI CGCG 1 cut(s) 3306
BspHI TCATGA 2 cut(s) 1171, 3889
BspLI GGNNCC 7 cut(s) 669, 1543, 2440, 2675, 2740, 2778, 3114
BspMAI CTGCAG 5 cut(s) 391, 472, 2596, 2644, 2766
BspPI GGATC 3 cut(s) 244, 355, 1169
BspQI GCTCTTC 2 cut(s) 2247, 3806
BspT107I GGYRCC 2 cut(s) 1541, 2673
BspTI CTTAAG 1 cut(s) 3841
BsrDI GCAATG 3 cut(s) 90, 1676, 3274
BsrFI RCCGGY 1 cut(s) 4055
BsrGI TGTACA 3 cut(s) 610, 3535, 3654
BsrI ACTGG 7 cut(s) 64, 1545, 2714, 2844, 3197, 3295, 3424
BssAI RCCGGY 1 cut(s) 4055
BssSI CACGAG 1 cut(s) 3359
BssT1I CCWWGG 5 cut(s) 1377, 2742, 3028, 3127, 3517
Bst2BI CACGAG 1 cut(s) 3359
Bst2UI CCWGG 7 cut(s) 234, 2354, 2904, 3489, 3606, 3714, 3814
Bst6I CTCTTC 2 cut(s) 2247, 3806
BstAFI CTTAAG 1 cut(s) 3841
BstAPI GCANNNNNTGC 5 cut(s) 326, 1994, 2966, 3224, 3647
BstAUI TGTACA 3 cut(s) 610, 3535, 3654
BstBAI YACGTR 2 cut(s) 783, 1430
BstC8I GCNNGC 8 cut(s) 360, 1100, 1366, 1978, 2240, 2372, 3772, 3884
BstDSI CCRYGG 4 cut(s) 671, 2832, 3127, 3517
BstEII GGTNACC 1 cut(s) 2826
BstFNI CGCG 1 cut(s) 3306
BstMAI GTCTC 5 cut(s) 41, 1332, 1739, 4068, 4165
BstNI CCWGG 7 cut(s) 234, 2354, 2904, 3489, 3606, 3714, 3814
BstNSI RCATGY 4 cut(s) 112, 291, 1850, 4130
BstPI GGTNACC 1 cut(s) 2826
BstSCI CCNGG 7 cut(s) 232, 2352, 2902, 3487, 3604, 3712, 3812
BstSFI CTRYAG 8 cut(s) 387, 468, 1743, 2149, 2592, 2640, 2762, 3321
BstSLI GKGCMC 1 cut(s) 2521
BstUI CGCG 1 cut(s) 3306
BstV2I GAAGAC 2 cut(s) 1859, 3966
BstX2I RGATCY 1 cut(s) 347
BstXI CCANNNNNNTGG 1 cut(s) 3365
BstYI RGATCY 1 cut(s) 347
BsuI GTATCC 1 cut(s) 39
BsuRI GGCC 8 cut(s) 155, 639, 922, 2454, 2497, 3027, 3113, 4080
BtgI CCRYGG 4 cut(s) 671, 2832, 3127, 3517
BtrI CACGTC 1 cut(s) 2615
BtsI GCAGTG 1 cut(s) 3970
BtsIMutI CAGTG 6 cut(s) 1552, 2837, 2978, 3208, 3650, 3970
Cac8I GCNNGC 8 cut(s) 360, 1100, 1366, 1978, 2240, 2372, 3772, 3884
CciI TCATGA 2 cut(s) 1171, 3889
Cfr10I RCCGGY 1 cut(s) 4055
Cfr13I GGNCC 6 cut(s) 115, 153, 638, 2346, 3112, 4079
CseI GACGC 1 cut(s) 30
CsiI ACCWGGT 1 cut(s) 3812
CspCI CAANNNNNGTGG 2 cut(s) 429, 464
DraIII CACNNNGTG 3 cut(s) 635, 1832, 2102
DriI GACNNNNNGTC 1 cut(s) 940
Eam1104I CTCTTC 2 cut(s) 2247, 3806
Eam1105I GACNNNNNGTC 1 cut(s) 940
EarI CTCTTC 2 cut(s) 2247, 3806
Ecl136II GAGCTC 2 cut(s) 3770, 3799
Eco130I CCWWGG 5 cut(s) 1377, 2742, 3028, 3127, 3517
Eco147I AGGCCT 1 cut(s) 2454
Eco24I GRGCYC 4 cut(s) 2327, 2980, 3772, 3801
Eco32I GATATC 2 cut(s) 1374, 2224
Eco47I GGWCC 2 cut(s) 115, 2346
Eco53kI GAGCTC 2 cut(s) 3770, 3799
Eco57I CTGAAG 3 cut(s) 529, 2496, 2624
Eco91I GGTNACC 1 cut(s) 2826
EcoICRI GAGCTC 2 cut(s) 3770, 3799
EcoO65I GGTNACC 1 cut(s) 2826
EcoRII CCWGG 7 cut(s) 232, 2352, 2902, 3487, 3604, 3712, 3812
EcoRV GATATC 2 cut(s) 1374, 2224
EcoT14I CCWWGG 5 cut(s) 1377, 2742, 3028, 3127, 3517
EcoT22I ATGCAT 4 cut(s) 1842, 1879, 2422, 3473
EcoT38I GRGCYC 4 cut(s) 2327, 2980, 3772, 3801
ErhI CCWWGG 5 cut(s) 1377, 2742, 3028, 3127, 3517
Esp3I CGTCTC 2 cut(s) 4068, 4165
FalI AAGNNNNNCTT 2 cut(s) 1233, 1265
FaqI GGGAC 1 cut(s) 2610
FauI CCCGC 1 cut(s) 2816
FauNDI CATATG 2 cut(s) 1515, 2277
FbaI TGATCA 4 cut(s) 1009, 1704, 3235, 3784
FriOI GRGCYC 4 cut(s) 2327, 2980, 3772, 3801
GsaI CCCAGC 3 cut(s) 1248, 1533, 3151
GsuI CTGGAG 5 cut(s) 216, 507, 3312, 3843, 4160
HaeIII GGCC 8 cut(s) 155, 639, 922, 2454, 2497, 3027, 3113, 4080
HapII CCGG 2 cut(s) 3924, 4056
HgaI GACGC 1 cut(s) 30
HincII GTYRAC 1 cut(s) 1153
HindII GTYRAC 1 cut(s) 1153
HindIII AAGCTT 1 cut(s) 77
HpaII CCGG 2 cut(s) 3924, 4056
HphI GGTGA 8 cut(s) 75, 621, 2025, 2205, 2807, 3434, 3533, 4078
Hpy166II GTNNAC 8 cut(s) 314, 514, 1153, 1666, 2093, 2895, 3358, 3672
Hpy8I GTNNAC 8 cut(s) 314, 514, 1153, 1666, 2093, 2895, 3358, 3672
Hpy99I CGWCG 1 cut(s) 24
HpyCH4IV ACGT 7 cut(s) 215, 310, 374, 782, 1289, 1429, 2614
HpySE526I ACGT 7 cut(s) 215, 310, 374, 782, 1289, 1429, 2614
KpnI GGTACC 1 cut(s) 2677
Ksp22I TGATCA 4 cut(s) 1009, 1704, 3235, 3784
LguI GCTCTTC 2 cut(s) 2247, 3806
LmnI GCTCC 8 cut(s) 235, 488, 667, 1864, 2110, 2288, 3638, 3689
MabI ACCWGGT 1 cut(s) 3812
MaeII ACGT 7 cut(s) 215, 310, 374, 782, 1289, 1429, 2614
MaeIII GTNAC 8 cut(s) 627, 1189, 1560, 2265, 2826, 3163, 3613, 3626
MfeI CAATTG 2 cut(s) 1659, 3555
MflI RGATCY 1 cut(s) 347
MhlI GDGCHC 7 cut(s) 778, 2107, 2327, 2521, 2980, 3772, 3801
MlyI GAGTC 9 cut(s) 43, 596, 1403, 2024, 2070, 2632, 3755, 3855, 3913
MmeI TCCRAC 5 cut(s) 93, 1031, 1570, 2466, 3979
Mph1103I ATGCAT 4 cut(s) 1842, 1879, 2422, 3473
MroXI GAANNNNTTC 2 cut(s) 2727, 2781
MslI CAYNNNNRTG 4 cut(s) 783, 1719, 1845, 3894
MspA1I CMGCKG 5 cut(s) 362, 458, 1391, 1574, 3147
MspCI CTTAAG 1 cut(s) 3841
MspI CCGG 2 cut(s) 3924, 4056
MspR9I CCNGG 7 cut(s) 234, 2354, 2904, 3489, 3606, 3714, 3814
MunI CAATTG 2 cut(s) 1659, 3555
Mva1269I GAATGC 1 cut(s) 3335
MvaI CCWGG 7 cut(s) 234, 2354, 2904, 3489, 3606, 3714, 3814
MvnI CGCG 1 cut(s) 3306
NcoI CCATGG 2 cut(s) 3127, 3517
NdeI CATATG 2 cut(s) 1515, 2277
NlaIV GGNNCC 7 cut(s) 669, 1543, 2440, 2675, 2740, 2778, 3114
NmuCI GTSAC 1 cut(s) 627
NsiI ATGCAT 4 cut(s) 1842, 1879, 2422, 3473
NspI RCATGY 4 cut(s) 112, 291, 1850, 4130
PagI TCATGA 2 cut(s) 1171, 3889
PasI CCCWGGG 1 cut(s) 3713
PceI AGGCCT 1 cut(s) 2454
PciI ACATGT 3 cut(s) 108, 287, 1846
PciSI GCTCTTC 2 cut(s) 2247, 3806
PctI GAATGC 1 cut(s) 3335
PdmI GAANNNNTTC 2 cut(s) 2727, 2781
PflFI GACNNNGTC 1 cut(s) 3680
PflMI CCANNNNNTGG 4 cut(s) 2042, 2071, 2995, 3203
PfoI TCCNGGA 2 cut(s) 232, 2902
PleI GAGTC 9 cut(s) 43, 596, 1402, 2024, 2069, 2631, 3755, 3854, 3912
PpsI GAGTC 9 cut(s) 43, 596, 1402, 2024, 2069, 2631, 3755, 3854, 3912
Ppu21I YACGTR 2 cut(s) 783, 1430
PscI ACATGT 3 cut(s) 108, 287, 1846
PsiI TTATAA 1 cut(s) 185
Psp124BI GAGCTC 2 cut(s) 3772, 3801
Psp1406I AACGTT 2 cut(s) 310, 1289
Psp6I CCWGG 7 cut(s) 232, 2352, 2902, 3487, 3604, 3712, 3812
PspEI GGTNACC 1 cut(s) 2826
PspFI CCCAGC 3 cut(s) 1244, 1529, 3147
PspGI CCWGG 7 cut(s) 232, 2352, 2902, 3487, 3604, 3712, 3812
PspN4I GGNNCC 7 cut(s) 669, 1543, 2440, 2675, 2740, 2778, 3114
PspPI GGNCC 6 cut(s) 115, 153, 638, 2346, 3112, 4079
PstI CTGCAG 5 cut(s) 391, 472, 2596, 2644, 2766
PsuI RGATCY 1 cut(s) 347
PsyI GACNNNGTC 1 cut(s) 3680
PvuII CAGCTG 4 cut(s) 362, 458, 1391, 1574
RseI CAYNNNNRTG 4 cut(s) 783, 1719, 1845, 3894
SacI GAGCTC 2 cut(s) 3772, 3801
SapI GCTCTTC 2 cut(s) 2247, 3806
Sau96I GGNCC 6 cut(s) 115, 153, 638, 2346, 3112, 4079
ScaI AGTACT 1 cut(s) 1960
SchI GAGTC 9 cut(s) 43, 596, 1403, 2024, 2070, 2632, 3755, 3855, 3913
ScrFI CCNGG 7 cut(s) 234, 2354, 2904, 3489, 3606, 3714, 3814
SduI GDGCHC 7 cut(s) 778, 2107, 2327, 2521, 2980, 3772, 3801
SexAI ACCWGGT 1 cut(s) 3812
SfcI CTRYAG 8 cut(s) 387, 468, 1743, 2149, 2592, 2640, 2762, 3321
SinI GGWCC 2 cut(s) 115, 2346
SmiMI CAYNNNNRTG 4 cut(s) 783, 1719, 1845, 3894
SmlI CTYRAG 5 cut(s) 1356, 1890, 2180, 3179, 3841
SmoI CTYRAG 5 cut(s) 1356, 1890, 2180, 3179, 3841
SseBI AGGCCT 1 cut(s) 2454
SsiI CCGC 8 cut(s) 1681, 1748, 2823, 3145, 3304, 3880, 3947, 4122
SspI AATATT 2 cut(s) 1477, 1728
SstI GAGCTC 2 cut(s) 3772, 3801
StuI AGGCCT 1 cut(s) 2454
StyD4I CCNGG 7 cut(s) 232, 2352, 2902, 3487, 3604, 3712, 3812
StyI CCWWGG 5 cut(s) 1377, 2742, 3028, 3127, 3517
TaiI ACGT 7 cut(s) 218, 313, 377, 785, 1292, 1432, 2617
TaqI TCGA 6 cut(s) 202, 417, 1307, 2220, 3476, 4196
TatI WGTACW 6 cut(s) 610, 766, 1958, 2892, 3535, 3654
TauI GCSGC 2 cut(s) 3882, 3949
TscAI CASTG 6 cut(s) 1552, 2844, 2978, 3208, 3657, 3977
TseFI GTSAC 1 cut(s) 627
Tsp45I GTSAC 1 cut(s) 627
TspGWI ACGGA 3 cut(s) 42, 1030, 1461
TspRI CASTG 6 cut(s) 1552, 2844, 2978, 3208, 3657, 3977
Tth111I GACNNNGTC 1 cut(s) 3680
Van91I CCANNNNNTGG 4 cut(s) 2042, 2071, 2995, 3203
Vha464I CTTAAG 1 cut(s) 3841
VpaK11BI GGWCC 2 cut(s) 115, 2346
XapI RAATTY 7 cut(s) 1057, 1220, 1326, 3076, 3251, 3283, 3741
XbaI TCTAGA 1 cut(s) 3764
XceI RCATGY 4 cut(s) 112, 291, 1850, 4130
XcmI CCANNNNNNNNNTGG 1 cut(s) 1646
XmaJI CCTAGG 1 cut(s) 3028
XmnI GAANNNNTTC 2 cut(s) 2727, 2781
ZrmI AGTACT 1 cut(s) 1960
Zsp2I ATGCAT 4 cut(s) 1842, 1879, 2422, 3473
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.