pycom16g05850

membrane

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0005229
Physical Location & Seq
Reverse (-)
3750301 .. 3754254
3954 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2154 bp
ATGCTTGTCTCCGCCCTGCTGACGTCACTCGCCATCAACTCCGGCCTCTGCGTCCTCTTCTTCACGCTCTACTCGATCCTCCGGAAGCAGCCGAGCAACTATGAGGTGTACATACCGCGGCTGCTCGCCGAGGGGGAGTCCGACAAGTCGAGTCGTTTCAACCTCGAGCGGCTGATTCCGTCGCCGTACTGGGTCAAGAGGGCCTGGCAGCTCACTGAGGACGACCTGCTGTCGTCCTCCGGCCTCGACGCCGTCGTTTTCATGCGGCTTATCAATTTTAGTCTCAGAGTGTTCCTGTTCGCCGGAGTTATCGGGATATTCGTCCTGCTTCCGGTCAACTGCTCCGGCAACCAGCTCGAGTATGTTGACTTCGCCGATCTGTCGAATAACTCTCTCGACGTGTTTACGATTTCGAATGTGAACAATGGCTCAAGCAAATTATGGATTCACTTTGCTGCTGTGTACCTTGTCACCATCTTTGTCTGCTGTCTACTTTATTATGAATTTAGATATATTTCTCAAAGACGGCTTGATTATTTTCAGTCATCCAAGCCTCAACCGCATCAGTTCACAATCTTAGTTCGCAGCATTCCTGTTCCTTCTGGGAGCAGTGTCAGTGAAAAAGTTGACAGCTTCTTTAGGGAGTATCACCCTTCAACGTATTTGTCACATATTGTTGTTCGTCGAACAAACAAAGTTAAAAAACTCCTTAAAGATACGAAAAAACTGTATACCAGGCTCCTTCACTTGCAATCGGATCCAAACCAGCAAAAGTATACACGATCCTGTTGTTTTGGATTGTTTGGACACAAGGTTAATCTTGTGGAACAGTATGAAAAGAAGCTTGAAGACGCAGAGGAGAATGTGAGATTGGAGCAATCAGAGGCTTCATTAGGAGGAGAAGAAGTTCGATCTGCTTTTGTTTCCTTCAAGTCTCGGTATGGTGCTGCAATTGCTCACCACTTGCAACAATCAACCAATCCTACCAACTGGGTCACAGAGCAAGCTCCTGAACCTCGTGATGTTTACTGGCCGTTCTTTTCGTCATCTTTCATCCGAAGATGGATATCTAAGCTTGTGGTTATACTTTTATGCATTGTCCTAACAATCTTGTTCCTTATTCCTGTTGTACTTGTCCAAGGTCTTACCAACCTAAACCAGCTGGAAGCTTGGCTTCCCTTTCTCGAAAGTGTTCTAACCATAACATTTGTTAGTCAAGTCATTACAGGATACCTTCCCAGTCTTATTCTTCTATTATTTCTGAAAATAGTGCCCCCCGCCATGGAGTTCCTTTCATCCATTCAAGGATATGTTTCTCTCAGTGATATACAAAAGAGTGCGTGTACAAAAGTACTTTGGTTCACGATATGGAACATTTTTTTTGCTACTGTATTTTCCGGATCAGTTCTCTCTAAGGTTTATCTCTTTCTTGACCCCAAGAATATTCCTTCAAAGCTAGGCGTTTCTGTTCCAGCACAGTCGTCTTTTTTCATTGCTTATGTCGTCACGTCAGGATGGACAAGTACTTCATCAGAACTCTTTCGCATAATTCCTCTGATATGGAGTCTGTTAAAAAGACCTTTTACCGACAGTAAAGATGATGAATTTGAAGTTCCAGCAATGAATTACCATAATCACATGCCAAGAGTTCTTTTCTTTGTACTTCTCGGCATCACATACTTCTTTCTAGCTCCACTGATTCTTCCCTTCCTCTTAGTGTACCTCTGTCTTGGATTCATCATCTTTCGCAACCAGTTCATAAACGTATATGCTCCTAGGTTTGAAACTGCAGGGAAGTTTTGGCCGATTGTGCATAATTCAATGATCTTCTCTCTGGTACTTATGCACGCTATTGCAGTTGGAATCTTTACAATCAAGAAGGTCAATCTAGCATCGACATTAACTTTTCCTCTTCCTGTATTCACACTTCTCTTCAATGAGTACTGCCGGAAGCGCTTCCTTCCAAATTTCGTTGCTTACCCTGCTGAGAGTTTGATACGAAAGGACAGGCAAGATGAGAACGATCCTACAATGCCTGAATTTCTCGATAAGTTGGTCACGGTCTATCAGGACCCTGCTTTGATGCCTATCAACTATTCTGGGAGCAATGACAGACTCCGCAGTCCTCTTTTATCTTCTGCTCGTCAAGCGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PHM7_cyt RSN1_7TM RSN1_TM

Protein Analysis

718

Amino Acids

82.08

Weight (kDa)

8.97

Isoelectric Point (pI)

45.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RSN1_TM PF13967 5 - 168 1.6e-38 Late exocytosis, associated with Golgi transport
PHM7_cyt PF14703 189 - 345 1.4e-37 Cytosolic domain of 10TM putative phosphate transporter
RSN1_7TM PF02714 358 - 623 3e-73 Calcium-dependent channel, 7TM region, putative phosphate
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 2121
AatII GACGTC 1 cut(s) 26
Acc36I ACCTGC 1 cut(s) 234
AccBSI CCGCTC 1 cut(s) 169
AccI GTMKAC 3 cut(s) 490, 731, 776
AccII CGCG 1 cut(s) 118
AccIII TCCGGA 2 cut(s) 81, 1397
AciI CCGC 8 cut(s) 12, 116, 118, 169, 265, 560, 1278, 2119
AclWI GGATC 6 cut(s) 70, 752, 765, 777, 1408, 2018
AcoI YGGCCR 2 cut(s) 1031, 1802
AcsI RAATTY 4 cut(s) 503, 1604, 1966, 2039
AcyI GRCGYC 2 cut(s) 23, 249
AfeI AGCGCT 1 cut(s) 1955
AfiI CCNNNNNNNGG 2 cut(s) 331, 1282
AflIII ACRYGT 1 cut(s) 399
AhdI GACNNNNNGTC 1 cut(s) 229
AjiI CACGTC 2 cut(s) 400, 1509
AjnI CCWGG 2 cut(s) 203, 734
AjuI GAANNNNNNNTTGG 2 cut(s) 854, 886
AloI GAACNNNNNNTCC 2 cut(s) 891, 923
Alw26I GTCTC 3 cut(s) 13, 287, 939
AlwI GGATC 6 cut(s) 70, 752, 765, 777, 1408, 2018
Ama87I CYCGRG 2 cut(s) 164, 356
Aor13HI TCCGGA 2 cut(s) 81, 1397
Aor51HI AGCGCT 1 cut(s) 1955
AoxI GGCC 5 cut(s) 43, 201, 241, 1031, 1802
ApeKI GCWGC 6 cut(s) 88, 121, 208, 455, 585, 947
ApoI RAATTY 4 cut(s) 503, 1604, 1966, 2039
Asp700I GAANNNNTTC 4 cut(s) 906, 1191, 1539, 1955
AspA2I CCTAGG 1 cut(s) 1775
AspLEI GCGC 1 cut(s) 1956
AspS9I GGNCC 2 cut(s) 201, 2071
AsuHPI GGTGA 3 cut(s) 463, 641, 950
AsuII TTCGAA 1 cut(s) 413
AvaI CYCGRG 2 cut(s) 164, 356
AvaII GGWCC 1 cut(s) 2071
AvrII CCTAGG 1 cut(s) 1775
BaeGI GKGCMC 1 cut(s) 1275
BamHI GGATCC 1 cut(s) 757
BarI GAAGNNNNNNTAC 4 cut(s) 353, 385, 1911, 1943
BauI CACGAG 1 cut(s) 1017
BbsI GAAGAC 1 cut(s) 855
BbvI GCAGC 6 cut(s) 100, 108, 220, 442, 597, 934
BccI CCATC 4 cut(s) 41, 482, 1056, 1509
BceAI ACGGC 4 cut(s) 169, 236, 542, 1018
BcgI CGANNNNNNTGC 2 cut(s) 337, 371
BciT130I CCWGG 2 cut(s) 205, 736
BciVI GTATCC 1 cut(s) 1223
BcoDI GTCTC 3 cut(s) 13, 287, 939
BfaI CTAG 4 cut(s) 1457, 1688, 1776, 1889
BfmI CTRYAG 1 cut(s) 1788
BfoI RGCGCY 1 cut(s) 1957
BfuAI ACCTGC 1 cut(s) 234
BfuI GTATCC 1 cut(s) 1223
BisI GCNGC 9 cut(s) 89, 119, 122, 170, 209, 266, 456, 586, 948
BlnI CCTAGG 1 cut(s) 1775
BlsI GCNGC 9 cut(s) 90, 120, 123, 171, 210, 267, 457, 587, 949
BmcAI AGTACT 3 cut(s) 1353, 1525, 1943
Bme1390I CCNGG 2 cut(s) 205, 736
Bme18I GGWCC 1 cut(s) 2071
BmeRI GACNNNNNGTC 1 cut(s) 229
BmeT110I CYCGRG 2 cut(s) 164, 356
BmgBI CACGTC 2 cut(s) 400, 1509
BmgT120I GGNCC 2 cut(s) 201, 2071
BmiI GGNNCC 3 cut(s) 740, 759, 2073
BmrFI CCNGG 2 cut(s) 205, 736
BmrI ACTGGG 3 cut(s) 199, 1000, 1233
BmsI GCATC 4 cut(s) 571, 1680, 1901, 2073
BmuI ACTGGG 3 cut(s) 199, 1000, 1233
BpiI GAAGAC 1 cut(s) 855
Bpu14I TTCGAA 1 cut(s) 413
BpuEI CTTGAG 1 cut(s) 415
BsaBI GATNNNNATC 2 cut(s) 1066, 2087
BsaHI GRCGYC 2 cut(s) 23, 249
BsaJI CCNNGG 5 cut(s) 116, 129, 1138, 1281, 1775
BsaWI WCCGGW 3 cut(s) 81, 331, 1397
BsaXI ACNNNNNCTCC 2 cut(s) 891, 921
Bsc4I CCNNNNNNNGG 2 cut(s) 331, 1282
Bse1I ACTGG 5 cut(s) 194, 995, 1034, 1239, 1753
Bse3DI GCAATG 3 cut(s) 1491, 1626, 2113
Bse8I GATNNNNATC 2 cut(s) 1066, 2087
BseAI TCCGGA 2 cut(s) 81, 1397
BseBI CCWGG 2 cut(s) 205, 736
BseDI CCNNGG 5 cut(s) 116, 129, 1138, 1281, 1775
BseGI GGATG 4 cut(s) 545, 1053, 1295, 1520
BseJI GATNNNNATC 2 cut(s) 1066, 2087
BseLI CCNNNNNNNGG 2 cut(s) 331, 1282
BseMI GCAATG 3 cut(s) 1491, 1626, 2113
BseMII CTCAG 4 cut(s) 207, 298, 1333, 1977
BseNI ACTGG 5 cut(s) 194, 995, 1034, 1239, 1753
BseRI GAGGAG 2 cut(s) 872, 912
BseSI GKGCMC 1 cut(s) 1275
BseXI GCAGC 6 cut(s) 100, 108, 220, 442, 597, 934
Bsh1236I CGCG 1 cut(s) 118
BshFI GGCC 5 cut(s) 45, 203, 243, 1033, 1804
BsiHKCI CYCGRG 2 cut(s) 164, 356
BsiSI CCGG 8 cut(s) 42, 82, 240, 303, 332, 345, 1398, 1948
BslI CCNNNNNNNGG 2 cut(s) 331, 1282
BsmAI GTCTC 3 cut(s) 13, 287, 939
BsmI GAATGC 1 cut(s) 588
BsnI GGCC 5 cut(s) 45, 203, 243, 1033, 1804
BsoBI CYCGRG 2 cut(s) 164, 356
Bsp119I TTCGAA 1 cut(s) 413
Bsp1286I GDGCHC 1 cut(s) 1275
Bsp13I TCCGGA 2 cut(s) 81, 1397
Bsp1407I TGTACA 2 cut(s) 108, 1343
Bsp143I GATC 8 cut(s) 75, 376, 757, 782, 911, 1400, 1824, 2023
Bsp19I CCATGG 1 cut(s) 1281
BspACI CCGC 8 cut(s) 12, 116, 118, 169, 265, 560, 1278, 2119
BspANI GGCC 5 cut(s) 45, 203, 243, 1033, 1804
BspCNI CTCAG 4 cut(s) 208, 297, 1332, 1978
BspEI TCCGGA 2 cut(s) 81, 1397
BspFNI CGCG 1 cut(s) 118
BspLI GGNNCC 3 cut(s) 740, 759, 2073
BspMAI CTGCAG 1 cut(s) 1792
BspMI ACCTGC 1 cut(s) 234
BspPI GGATC 6 cut(s) 70, 752, 765, 777, 1408, 2018
BspT104I TTCGAA 1 cut(s) 413
BsrBI CCGCTC 1 cut(s) 169
BsrDI GCAATG 3 cut(s) 1491, 1626, 2113
BsrGI TGTACA 2 cut(s) 108, 1343
BsrI ACTGG 5 cut(s) 194, 995, 1034, 1239, 1753
BssECI CCNNGG 5 cut(s) 116, 129, 1138, 1281, 1775
BssMI GATC 8 cut(s) 75, 376, 757, 782, 911, 1400, 1824, 2023
BssNAI GTATAC 2 cut(s) 732, 777
BssNI GRCGYC 2 cut(s) 23, 249
BssSI CACGAG 1 cut(s) 1017
BssT1I CCWWGG 3 cut(s) 1138, 1281, 1775
Bst1107I GTATAC 2 cut(s) 732, 777
Bst2BI CACGAG 1 cut(s) 1017
Bst2UI CCWGG 2 cut(s) 205, 736
Bst4CI ACNGT 6 cut(s) 729, 831, 1390, 1479, 1592, 2062
Bst6I CTCTTC 3 cut(s) 62, 1917, 1937
BstACI GRCGYC 2 cut(s) 23, 249
BstAUI TGTACA 2 cut(s) 108, 1343
BstBI TTCGAA 1 cut(s) 413
BstC8I GCNNGC 3 cut(s) 126, 1005, 1848
BstDEI CTNAG 8 cut(s) 216, 284, 577, 1071, 1319, 1413, 1714, 1986
BstDSI CCRYGG 2 cut(s) 116, 1281
BstF5I GGATG 4 cut(s) 545, 1053, 1295, 1520
BstFNI CGCG 1 cut(s) 118
BstH2I RGCGCY 1 cut(s) 1957
BstHHI GCGC 1 cut(s) 1956
BstKTI GATC 8 cut(s) 78, 379, 760, 785, 914, 1403, 1827, 2026
BstMAI GTCTC 3 cut(s) 13, 287, 939
BstMBI GATC 8 cut(s) 75, 376, 757, 782, 911, 1400, 1824, 2023
BstMWI GCNNNNNNNGC 6 cut(s) 559, 953, 1810, 1953, 1982, 2147
BstNI CCWGG 2 cut(s) 205, 736
BstNSI RCATGY 1 cut(s) 1642
BstSCI CCNGG 2 cut(s) 203, 734
BstSFI CTRYAG 1 cut(s) 1788
BstSLI GKGCMC 1 cut(s) 1275
BstUI CGCG 1 cut(s) 118
BstV1I GCAGC 6 cut(s) 100, 108, 220, 442, 597, 934
BstV2I GAAGAC 1 cut(s) 855
BstX2I RGATCY 1 cut(s) 757
BstYI RGATCY 1 cut(s) 757
BstZ17I GTATAC 2 cut(s) 732, 777
BsuI GTATCC 1 cut(s) 1223
BsuRI GGCC 5 cut(s) 45, 203, 243, 1033, 1804
BtgI CCRYGG 2 cut(s) 116, 1281
BtrI CACGTC 2 cut(s) 400, 1509
BtsCI GGATG 4 cut(s) 545, 1053, 1295, 1520
BtsI GCAGTG 1 cut(s) 616
BtsIMutI CAGTG 5 cut(s) 213, 616, 622, 1327, 1694
BveI ACCTGC 1 cut(s) 234
Cac8I GCNNGC 3 cut(s) 126, 1005, 1848
CfoI GCGC 1 cut(s) 1956
Cfr13I GGNCC 2 cut(s) 201, 2071
Cfr42I CCGCGG 1 cut(s) 119
CseI GACGC 3 cut(s) 40, 257, 860
CviAII CATG 3 cut(s) 262, 1282, 1639
DdeI CTNAG 8 cut(s) 216, 284, 577, 1071, 1319, 1413, 1714, 1986
DpnI GATC 8 cut(s) 77, 378, 759, 784, 913, 1402, 1826, 2025
DpnII GATC 8 cut(s) 75, 376, 757, 782, 911, 1400, 1824, 2023
DrdI GACNNNNNNGTC 1 cut(s) 2121
DriI GACNNNNNGTC 1 cut(s) 229
DseDI GACNNNNNNGTC 1 cut(s) 2121
EaeI YGGCCR 2 cut(s) 1031, 1802
Eam1104I CTCTTC 3 cut(s) 62, 1917, 1937
Eam1105I GACNNNNNGTC 1 cut(s) 229
EarI CTCTTC 3 cut(s) 62, 1917, 1937
Eco130I CCWWGG 3 cut(s) 1138, 1281, 1775
Eco32I GATATC 1 cut(s) 1068
Eco47I GGWCC 1 cut(s) 2071
Eco47III AGCGCT 1 cut(s) 1955
Eco88I CYCGRG 2 cut(s) 164, 356
EcoO109I RGGNCCY 2 cut(s) 201, 2071
EcoRII CCWGG 2 cut(s) 203, 734
EcoRV GATATC 1 cut(s) 1068
EcoT14I CCWWGG 3 cut(s) 1138, 1281, 1775
EcoT22I ATGCAT 1 cut(s) 1097
ErhI CCWWGG 3 cut(s) 1138, 1281, 1775
FaeI CATG 3 cut(s) 265, 1285, 1642
FalI AAGNNNNNCTT 2 cut(s) 1158, 1190
FatI CATG 3 cut(s) 261, 1281, 1638
FauI CCCGC 1 cut(s) 1285
FblI GTMKAC 3 cut(s) 490, 731, 776
Fnu4HI GCNGC 9 cut(s) 89, 119, 122, 170, 209, 266, 456, 586, 948
FokI GGATG 4 cut(s) 532, 1040, 1282, 1527
Fsp4HI GCNGC 9 cut(s) 89, 119, 122, 170, 209, 266, 456, 586, 948
FspBI CTAG 4 cut(s) 1457, 1688, 1776, 1889
GlaI GCGC 1 cut(s) 1955
GluI GCNGC 9 cut(s) 89, 119, 122, 170, 209, 266, 456, 586, 948
HaeII RGCGCY 1 cut(s) 1957
HaeIII GGCC 5 cut(s) 45, 203, 243, 1033, 1804
HapII CCGG 8 cut(s) 42, 82, 240, 303, 332, 345, 1398, 1948
HgaI GACGC 3 cut(s) 40, 257, 860
HhaI GCGC 1 cut(s) 1956
Hin1I GRCGYC 2 cut(s) 23, 249
Hin1II CATG 3 cut(s) 265, 1285, 1642
Hin6I GCGC 1 cut(s) 1954
HinP1I GCGC 1 cut(s) 1954
HincII GTYRAC 3 cut(s) 337, 367, 628
HindII GTYRAC 3 cut(s) 337, 367, 628
HindIII AAGCTT 3 cut(s) 842, 1073, 1167
HinfI GANTC 9 cut(s) 137, 151, 175, 445, 1564, 1699, 1734, 1863, 2115
HpaII CCGG 8 cut(s) 42, 82, 240, 303, 332, 345, 1398, 1948
HphI GGTGA 3 cut(s) 463, 641, 950
Hpy188I TCNGA 8 cut(s) 142, 287, 757, 883, 1058, 1263, 1534, 1557
Hpy99I CGWCG 5 cut(s) 184, 251, 257, 401, 687
HpyAV CCTTC 9 cut(s) 609, 663, 752, 937, 1244, 1458, 1717, 1873, 1970
HpyCH4III ACNGT 6 cut(s) 729, 831, 1390, 1479, 1592, 2062
HpyCH4IV ACGT 5 cut(s) 23, 399, 659, 1508, 1764
HpyCH4V TGCA 8 cut(s) 751, 950, 967, 1095, 1790, 1813, 1846, 1856
HpyF10VI GCNNNNNNNGC 6 cut(s) 559, 953, 1810, 1953, 1982, 2147
HpyF3I CTNAG 8 cut(s) 216, 284, 577, 1071, 1319, 1413, 1714, 1986
HpySE526I ACGT 5 cut(s) 23, 399, 659, 1508, 1764
Hsp92I GRCGYC 2 cut(s) 23, 249
Hsp92II CATG 3 cut(s) 265, 1285, 1642
HspAI GCGC 1 cut(s) 1954
Kpn2I TCCGGA 2 cut(s) 81, 1397
KspI CCGCGG 1 cut(s) 119
Kzo9I GATC 8 cut(s) 75, 376, 757, 782, 911, 1400, 1824, 2023
LmnI GCTCC 8 cut(s) 347, 606, 744, 874, 1012, 1696, 1777, 2103
Lsp1109I GCAGC 6 cut(s) 100, 108, 220, 442, 597, 934
LweI GCATC 4 cut(s) 571, 1680, 1901, 2073
MaeI CTAG 4 cut(s) 1457, 1688, 1776, 1889
MaeII ACGT 5 cut(s) 23, 399, 659, 1508, 1764
MaeIII GTNAC 6 cut(s) 24, 469, 666, 994, 1504, 2056
MalI GATC 8 cut(s) 77, 378, 759, 784, 913, 1402, 1826, 2025
MbiI CCGCTC 1 cut(s) 169
MboI GATC 8 cut(s) 75, 376, 757, 782, 911, 1400, 1824, 2023
MfeI CAATTG 1 cut(s) 951
MflI RGATCY 1 cut(s) 757
MhlI GDGCHC 1 cut(s) 1275
MlyI GAGTC 4 cut(s) 146, 160, 1573, 2109
MmeI TCCRAC 2 cut(s) 165, 1840
Mph1103I ATGCAT 1 cut(s) 1097
MroI TCCGGA 2 cut(s) 81, 1397
MroXI GAANNNNTTC 4 cut(s) 906, 1191, 1539, 1955
MseI TTAA 5 cut(s) 699, 711, 816, 1571, 1901
MspA1I CMGCKG 2 cut(s) 118, 1162
MspI CCGG 8 cut(s) 42, 82, 240, 303, 332, 345, 1398, 1948
MspR9I CCNGG 2 cut(s) 205, 736
MunI CAATTG 1 cut(s) 951
Mva1269I GAATGC 1 cut(s) 588
MvaI CCWGG 2 cut(s) 205, 736
MvnI CGCG 1 cut(s) 118
MwoI GCNNNNNNNGC 6 cut(s) 559, 953, 1810, 1953, 1982, 2147
NcoI CCATGG 1 cut(s) 1281
NdeII GATC 8 cut(s) 75, 376, 757, 782, 911, 1400, 1824, 2023
NlaIII CATG 3 cut(s) 265, 1285, 1642
NlaIV GGNNCC 3 cut(s) 740, 759, 2073
NmeAIII GCCGAG 3 cut(s) 117, 154, 1647
NmuCI GTSAC 6 cut(s) 24, 469, 666, 994, 1504, 2056
NsiI ATGCAT 1 cut(s) 1097
NspI RCATGY 1 cut(s) 1642
NspV TTCGAA 1 cut(s) 413
PaeR7I CTCGAG 2 cut(s) 164, 356
PcsI WCGNNNNNNNCGW 3 cut(s) 71, 252, 318
PctI GAATGC 1 cut(s) 588
PdmI GAANNNNTTC 4 cut(s) 906, 1191, 1539, 1955
PfeI GAWTC 5 cut(s) 175, 445, 1699, 1734, 1863
PflFI GACNNNGTC 1 cut(s) 251
PkrI GCNGC 9 cut(s) 90, 120, 123, 171, 210, 267, 457, 587, 949
PleI GAGTC 4 cut(s) 145, 159, 1572, 2109
PpsI GAGTC 4 cut(s) 145, 159, 1572, 2109
PpuMI RGGWCCY 1 cut(s) 2071
Psp5II RGGWCCY 1 cut(s) 2071
Psp6I CCWGG 2 cut(s) 203, 734
PspGI CCWGG 2 cut(s) 203, 734
PspN4I GGNNCC 3 cut(s) 740, 759, 2073
PspPI GGNCC 2 cut(s) 201, 2071
PspPPI RGGWCCY 1 cut(s) 2071
PspXI VCTCGAGB 2 cut(s) 164, 356
PstI CTGCAG 1 cut(s) 1792
PsuI RGATCY 1 cut(s) 757
PsyI GACNNNGTC 1 cut(s) 251
PvuII CAGCTG 1 cut(s) 1162
SacII CCGCGG 1 cut(s) 119
SaqAI TTAA 5 cut(s) 699, 711, 816, 1571, 1901
SatI GCNGC 9 cut(s) 89, 119, 122, 170, 209, 266, 456, 586, 948
Sau3AI GATC 8 cut(s) 75, 376, 757, 782, 911, 1400, 1824, 2023
Sau96I GGNCC 2 cut(s) 201, 2071
ScaI AGTACT 3 cut(s) 1353, 1525, 1943
SchI GAGTC 4 cut(s) 146, 160, 1573, 2109
ScrFI CCNGG 2 cut(s) 205, 736
SduI GDGCHC 1 cut(s) 1275
SfaNI GCATC 4 cut(s) 571, 1680, 1901, 2073
SfcI CTRYAG 1 cut(s) 1788
Sfr274I CTCGAG 2 cut(s) 164, 356
Sfr303I CCGCGG 1 cut(s) 119
SfuI TTCGAA 1 cut(s) 413
SgrBI CCGCGG 1 cut(s) 119
SinI GGWCC 1 cut(s) 2071
SlaI CTCGAG 2 cut(s) 164, 356
SmlI CTYRAG 3 cut(s) 164, 356, 430
SmoI CTYRAG 3 cut(s) 164, 356, 430
SsiI CCGC 8 cut(s) 12, 116, 118, 169, 265, 560, 1278, 2119
SspI AATATT 1 cut(s) 1444
SspMI CTAG 4 cut(s) 1457, 1688, 1776, 1889
StyD4I CCNGG 2 cut(s) 203, 734
StyI CCWWGG 3 cut(s) 1138, 1281, 1775
TaaI ACNGT 6 cut(s) 729, 831, 1390, 1479, 1592, 2062
TaiI ACGT 5 cut(s) 26, 402, 662, 1511, 1767
TatI WGTACW 7 cut(s) 108, 1129, 1343, 1351, 1523, 1660, 1941
TauI GCSGC 3 cut(s) 121, 172, 268
TfiI GAWTC 5 cut(s) 175, 445, 1699, 1734, 1863
Tru1I TTAA 5 cut(s) 699, 711, 816, 1571, 1901
Tru9I TTAA 5 cut(s) 699, 711, 816, 1571, 1901
TscAI CASTG 5 cut(s) 220, 616, 622, 1327, 1701
TseFI GTSAC 6 cut(s) 24, 469, 666, 994, 1504, 2056
TseI GCWGC 6 cut(s) 88, 121, 208, 455, 585, 947
Tsp45I GTSAC 6 cut(s) 24, 469, 666, 994, 1504, 2056
TspGWI ACGGA 1 cut(s) 168
TspRI CASTG 5 cut(s) 220, 616, 622, 1327, 1701
Tth111I GACNNNGTC 1 cut(s) 251
VpaK11BI GGWCC 1 cut(s) 2071
XapI RAATTY 4 cut(s) 503, 1604, 1966, 2039
XceI RCATGY 1 cut(s) 1642
XhoI CTCGAG 2 cut(s) 164, 356
XmaJI CCTAGG 1 cut(s) 1775
XmiI GTMKAC 3 cut(s) 490, 731, 776
XmnI GAANNNNTTC 4 cut(s) 906, 1191, 1539, 1955
XspI CTAG 4 cut(s) 1457, 1688, 1776, 1889
ZraI GACGTC 1 cut(s) 24
ZrmI AGTACT 3 cut(s) 1353, 1525, 1943
Zsp2I ATGCAT 1 cut(s) 1097
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.