pycom16g06230

50S ribosomal protein L18

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0004153
Physical Location & Seq
Forward (+)
3989049 .. 3989866
818 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 510 bp
ATGGCCCTTACGAATGCTGCTGCTCTCAAATTCTCGACTTCTGCATTTTTGGGTCTCCGTTCGGCAACTTCCAACTTTCAAATTCAACCCATGCAGCAGACGAGAAGAACAAGATTCTCGGCGAAGCCGTTAGTTATGAGAGCGAGGGGGAATGCCAGGACGGATAGTGCGAAAACTCGAAACAGAAGAATGCAGAAGAAGTACAATGGCACGTCTATGAGACCGAGGCTTTCGGTGTTCTGTTCAGATAAACAGTTGTATGCAATGCTGGTAGATGACCAAAACAAGAACTGTTTGTTTTACGGAAGCACCCTGCAGAAATCTATTCGTCGGAATGCCGATTGTAGTACCAGCGAAGCTGCTAAACTCCTGGGCGAGGAGCTCGTCAAGGCCTGCAACAATCTCAACATACACGAAATATCATCGTATGATCGCAATGGTTTTGCTACCGGGGAAAGGATTCAGGCTTTCGAGATCGCAATTTCTCGGCATGGTTTCTTGCCAAGATAG

Protein Analysis

170

Amino Acids

19.07

Weight (kDa)

10.49

Isoelectric Point (pI)

42.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L18p PF00861 53 - 159 8.8e-18 Ribosomal L18 of archaea, bacteria, mitoch. and chloroplast
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcsI RAATTY 2 cut(s) 29, 81
AfaI GTAC 2 cut(s) 203, 349
AfiI CCNNNNNNNGG 2 cut(s) 376, 456
AgsI TTSAA 2 cut(s) 80, 86
AjiI CACGTC 1 cut(s) 213
AjnI CCWGG 2 cut(s) 155, 369
AluBI AGCT 2 cut(s) 359, 382
AluI AGCT 2 cut(s) 359, 382
Alw21I GWGCWC 1 cut(s) 384
Alw26I GTCTC 2 cut(s) 59, 214
AoxI GGCC 2 cut(s) 3, 390
ApeKI GCWGC 4 cut(s) 17, 20, 94, 359
ApoI RAATTY 2 cut(s) 29, 81
Asp700I GAANNNNTTC 1 cut(s) 459
AspS9I GGNCC 1 cut(s) 4
AsuC2I CCSGG 1 cut(s) 451
BanII GRGCYC 1 cut(s) 384
Bbv12I GWGCWC 1 cut(s) 384
BbvI GCAGC 4 cut(s) 4, 7, 106, 346
BceAI ACGGC 1 cut(s) 112
BciT130I CCWGG 2 cut(s) 157, 371
BcnI CCSGG 1 cut(s) 451
BcoDI GTCTC 2 cut(s) 59, 214
BfmI CTRYAG 1 cut(s) 314
BisI GCNGC 4 cut(s) 18, 21, 95, 360
BlsI GCNGC 4 cut(s) 19, 22, 96, 361
Bme1390I CCNGG 3 cut(s) 157, 371, 451
BmgBI CACGTC 1 cut(s) 213
BmgT120I GGNCC 1 cut(s) 4
BmrFI CCNGG 3 cut(s) 157, 371, 451
BpuMI CCSGG 1 cut(s) 451
BsaI GGTCTC 2 cut(s) 59, 214
BsaJI CCNNGG 3 cut(s) 224, 370, 450
Bsc4I CCNNNNNNNGG 2 cut(s) 376, 456
Bse3DI GCAATG 2 cut(s) 270, 442
BseBI CCWGG 2 cut(s) 157, 371
BseDI CCNNGG 3 cut(s) 224, 370, 450
BseLI CCNNNNNNNGG 2 cut(s) 376, 456
BseMI GCAATG 2 cut(s) 270, 442
BseRI GAGGAG 1 cut(s) 392
BseXI GCAGC 4 cut(s) 4, 7, 106, 346
BshFI GGCC 2 cut(s) 5, 392
BsiHKAI GWGCWC 1 cut(s) 384
BsiSI CCGG 1 cut(s) 450
BslI CCNNNNNNNGG 2 cut(s) 376, 456
BsmAI GTCTC 2 cut(s) 59, 214
BsmI GAATGC 4 cut(s) 19, 157, 195, 340
BsnI GGCC 2 cut(s) 5, 392
Bso31I GGTCTC 2 cut(s) 59, 214
Bsp1286I GDGCHC 1 cut(s) 384
Bsp143I GATC 2 cut(s) 430, 474
BspANI GGCC 2 cut(s) 5, 392
BspMAI CTGCAG 1 cut(s) 318
BspTNI GGTCTC 2 cut(s) 59, 214
BsrDI GCAATG 2 cut(s) 270, 442
BssECI CCNNGG 3 cut(s) 224, 370, 450
BssMI GATC 2 cut(s) 430, 474
Bst2UI CCWGG 2 cut(s) 157, 371
Bst4CI ACNGT 2 cut(s) 255, 293
BstC8I GCNNGC 1 cut(s) 394
BstENI CCTNNNNNAGG 1 cut(s) 374
BstKTI GATC 2 cut(s) 433, 477
BstMAI GTCTC 2 cut(s) 59, 214
BstMBI GATC 2 cut(s) 430, 474
BstNI CCWGG 2 cut(s) 157, 371
BstSCI CCNGG 3 cut(s) 155, 369, 449
BstSFI CTRYAG 1 cut(s) 314
BstV1I GCAGC 4 cut(s) 4, 7, 106, 346
BsuRI GGCC 2 cut(s) 5, 392
BtrI CACGTC 1 cut(s) 213
Cac8I GCNNGC 1 cut(s) 394
Cfr13I GGNCC 1 cut(s) 4
Csp6I GTAC 2 cut(s) 202, 348
CviAII CATG 2 cut(s) 91, 491
CviJI RGCY 7 cut(s) 5, 127, 229, 359, 382, 392, 467
CviKI_1 RGCY 7 cut(s) 5, 127, 229, 359, 382, 392, 467
CviQI GTAC 2 cut(s) 202, 348
DpnI GATC 2 cut(s) 432, 476
DpnII GATC 2 cut(s) 430, 474
Ecl136II GAGCTC 1 cut(s) 382
Eco147I AGGCCT 1 cut(s) 392
Eco24I GRGCYC 1 cut(s) 384
Eco31I GGTCTC 2 cut(s) 59, 214
Eco53kI GAGCTC 1 cut(s) 382
EcoICRI GAGCTC 1 cut(s) 382
EcoNI CCTNNNNNAGG 1 cut(s) 374
EcoRII CCWGG 2 cut(s) 155, 369
EcoT38I GRGCYC 1 cut(s) 384
FaeI CATG 2 cut(s) 94, 494
FaiI YATR 7 cut(s) 92, 137, 218, 261, 410, 429, 492
FatI CATG 2 cut(s) 90, 490
Fnu4HI GCNGC 4 cut(s) 18, 21, 95, 360
FriOI GRGCYC 1 cut(s) 384
Fsp4HI GCNGC 4 cut(s) 18, 21, 95, 360
GluI GCNGC 4 cut(s) 18, 21, 95, 360
HaeIII GGCC 2 cut(s) 5, 392
HapII CCGG 1 cut(s) 450
Hin1II CATG 2 cut(s) 94, 494
HinfI GANTC 2 cut(s) 114, 460
HpaII CCGG 1 cut(s) 450
Hpy188I TCNGA 2 cut(s) 247, 333
Hpy188III TCNNGA 2 cut(s) 34, 472
Hpy99I CGWCG 1 cut(s) 333
HpyCH4III ACNGT 2 cut(s) 255, 293
HpyCH4IV ACGT 1 cut(s) 212
HpyCH4V TGCA 6 cut(s) 44, 94, 193, 263, 316, 396
HpySE526I ACGT 1 cut(s) 212
Hsp92II CATG 2 cut(s) 94, 494
Kzo9I GATC 2 cut(s) 430, 474
LmnI GCTCC 1 cut(s) 379
Lsp1109I GCAGC 4 cut(s) 4, 7, 106, 346
MaeII ACGT 1 cut(s) 212
MalI GATC 2 cut(s) 432, 476
MboI GATC 2 cut(s) 430, 474
MboII GAAGA 3 cut(s) 117, 198, 208
MhlI GDGCHC 1 cut(s) 384
MluCI AATT 3 cut(s) 29, 81, 480
MmeI TCCRAC 2 cut(s) 96, 311
MnlI CCTC 3 cut(s) 138, 219, 370
MroXI GAANNNNTTC 1 cut(s) 459
MslI CAYNNNNRTG 1 cut(s) 215
MspI CCGG 1 cut(s) 450
MspR9I CCNGG 3 cut(s) 157, 371, 451
Mva1269I GAATGC 4 cut(s) 19, 157, 195, 340
MvaI CCWGG 2 cut(s) 157, 371
NciI CCSGG 1 cut(s) 451
NdeII GATC 2 cut(s) 430, 474
NlaIII CATG 2 cut(s) 94, 494
NmeAIII GCCGAG 2 cut(s) 98, 466
PceI AGGCCT 1 cut(s) 392
PcsI WCGNNNNNNNCGW 2 cut(s) 125, 167
PctI GAATGC 4 cut(s) 19, 157, 195, 340
PdmI GAANNNNTTC 1 cut(s) 459
PfeI GAWTC 2 cut(s) 114, 460
PkrI GCNGC 4 cut(s) 19, 22, 96, 361
Psp124BI GAGCTC 1 cut(s) 384
Psp6I CCWGG 2 cut(s) 155, 369
PspGI CCWGG 2 cut(s) 155, 369
PspPI GGNCC 1 cut(s) 4
PstI CTGCAG 1 cut(s) 318
RsaI GTAC 2 cut(s) 203, 349
RsaNI GTAC 2 cut(s) 202, 348
RseI CAYNNNNRTG 1 cut(s) 215
SacI GAGCTC 1 cut(s) 384
SatI GCNGC 4 cut(s) 18, 21, 95, 360
Sau3AI GATC 2 cut(s) 430, 474
Sau96I GGNCC 1 cut(s) 4
ScrFI CCNGG 3 cut(s) 157, 371, 451
SduI GDGCHC 1 cut(s) 384
SetI ASST 3 cut(s) 215, 361, 384
SfcI CTRYAG 1 cut(s) 314
SmiMI CAYNNNNRTG 1 cut(s) 215
Sse9I AATT 3 cut(s) 29, 81, 480
SseBI AGGCCT 1 cut(s) 392
SstI GAGCTC 1 cut(s) 384
StuI AGGCCT 1 cut(s) 392
StyD4I CCNGG 3 cut(s) 155, 369, 449
TaaI ACNGT 2 cut(s) 255, 293
TaiI ACGT 1 cut(s) 215
TaqI TCGA 3 cut(s) 35, 178, 471
TaqII GACCGA 1 cut(s) 238
TasI AATT 3 cut(s) 29, 81, 480
TatI WGTACW 1 cut(s) 201
TfiI GAWTC 2 cut(s) 114, 460
TseI GCWGC 4 cut(s) 17, 20, 94, 359
TspGWI ACGGA 3 cut(s) 47, 176, 318
XagI CCTNNNNNAGG 1 cut(s) 374
XapI RAATTY 2 cut(s) 29, 81
XmnI GAANNNNTTC 1 cut(s) 459
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.