pycom16g06430

Protein of unknown function (DUF616)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0005812
Physical Location & Seq
Forward (+)
4134419 .. 4137168
2750 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1455 bp
ATGGTAAAAAGTGAAAAAAAAAGATCTCCGACCAGGAAAACCCGCCATGAAAACAGCTCCATAGACGACGGTGGCGAATCGCTGTTGCCGGAGGAGATGAGGAGAGGTATAATCGGCGCGTTGTTGAAGTATCAGGGTGAGCACGGCACTCGCCTTGTTCGCAAAGGAAGGAGGCTAGGTCGAATCGCCAGGAACAAATTCTGGTTCTGGGTTTTTCTCTTTGCAGCCATTTTTTTTATTCACTTGCTGCTCGTCGGCTTCAAAATGCATCCTCCTGCTAAACTAGAAGGTAGTGGTAGTAGTATTAGTATTTCGGTTCACCCAGAAATTTTGCCGTCTGGTGTTGGAAAACCACCCAGAAGCAAGCGTCGTGCCAAGTATTTGCCTTGTGAAGTTGGGTTACTGGAATCAGTTGAGTATCTTGTTGAACCCAGGGAAGTCAGGAACTTCACATATTTTTCACTGGAATACATTGACCAAGAGGGATTACCTTCTCGTAGTAATATGTACGAACCTAGATTTGGAGGGCATCAGACACTTGAGGAAAGAGAGCAATCCTTCTTTGCGAAAAATCAAACTCTGCATTGTGGATTTGTCAAGGGACCCCTGGGATTCTCAAGCTCTGGATTTGATTTACACGTTAAGGACAAGGCATACATGAATGGCTGTAAGATTGTAGTATCTTCTTGCATTTTCGGAAGCTCTGACTTTCTAAGGAGGCCTACAACCAAAAAGATGAGCGAATATTCCAAGAAAAATGTTTGTTTTGTAATGTTTGTGGATGAGCAAACATTGGCAAAACTATCAACAGAGGGACATGTTCCTGATGATACAGGACGAATTGGCTTGTGGAGGATAGTCATAGTGAAGAATTTACCATATGAGGATATGCGCAGAACTGGAAAGGTGCCAAAACTTTTATCACATCGCCTTTTCCCCAATTCATGGTATTCCATTTGGCTTGACAGCAAGATGCGACTCAATACTGATCCGATGCTGATTATTGAATACTTTTTATGGCGCACAAAATCAGAGTATGCCATTTCCAACCATTATGGTCGCCATTGTGTCTGGGACGAGGTACTCCAAAACAAGCGTCTAGATAAGTACAATCACACAGCTATTGATGAACAGTTTACATTTTACCAGTCTGATGGCCTCACCAAGTTTGACCCTTCAGACCCGAATACTCCTCTTCCAAGTTACGTTCCTGAAGGTTCATTTATAGTCCGGGCACATACACCAATTTCAAATTTGTTTTCCTGCCTTTGGTTTAATGAAGTTAACAGGTTTACCTCCCGTGATCAACTAAGCTTCGCATATACTTACCTAAAATTGAGAAGAATGAACCCAGAGAGACCATTCTACCTAAACATGTTCAAGGACTGTGAACGTAGAGCACTAACGAAGCTATTCCAGCACAGGGAAGCACCGCCTTCTCCACCCATTACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF616

Protein Analysis

485

Amino Acids

56.26

Weight (kDa)

9.51

Isoelectric Point (pI)

54.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TOD1_MUCI70 PF04765 155 - 474 1.7e-143 TOD1/MUCI70, glycosyltransferase-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 893
AccB1I GGYRCC 1 cut(s) 907
AccB7I CCANNNNNTGG 1 cut(s) 945
AccII CGCG 1 cut(s) 119
AciI CCGC 2 cut(s) 43, 1433
AclWI GGATC 1 cut(s) 983
AcsI RAATTY 4 cut(s) 197, 327, 871, 1252
AcuI CTGAAG 2 cut(s) 1161, 1233
AfaI GTAC 3 cut(s) 509, 1083, 1109
AfiI CCNNNNNNNGG 4 cut(s) 521, 945, 1269, 1423
AflIII ACRYGT 3 cut(s) 637, 817, 1374
AgsI TTSAA 6 cut(s) 127, 262, 428, 1007, 1251, 1381
AjnI CCWGG 4 cut(s) 32, 188, 431, 606
AjuI GAANNNNNNNTTGG 2 cut(s) 504, 536
AluBI AGCT 6 cut(s) 57, 621, 702, 1121, 1314, 1411
AluI AGCT 6 cut(s) 57, 621, 702, 1121, 1314, 1411
Alw21I GWGCWC 2 cut(s) 144, 1402
Alw26I GTCTC 1 cut(s) 1351
AlwI GGATC 1 cut(s) 983
AoxI GGCC 2 cut(s) 719, 1156
ApeKI GCWGC 2 cut(s) 224, 247
ApoI RAATTY 4 cut(s) 197, 327, 871, 1252
ArsI GACNNNNNNTTYG 2 cut(s) 1081, 1113
Asp700I GAANNNNTTC 2 cut(s) 197, 1412
AspLEI GCGC 3 cut(s) 119, 894, 1023
AspS9I GGNCC 1 cut(s) 602
AsuC2I CCSGG 1 cut(s) 1232
AsuHPI GGTGA 3 cut(s) 149, 311, 1153
AvaII GGWCC 1 cut(s) 602
BaeGI GKGCMC 1 cut(s) 1237
BanI GGYRCC 1 cut(s) 907
BarI GAAGNNNNNNTAC 2 cut(s) 384, 416
Bbv12I GWGCWC 2 cut(s) 144, 1402
BbvI GCAGC 2 cut(s) 234, 236
BccI CCATC 1 cut(s) 1148
BceAI ACGGC 2 cut(s) 160, 319
BcgI CGANNNNNNTGC 2 cut(s) 131, 165
BciT130I CCWGG 4 cut(s) 34, 190, 433, 608
BclI TGATCA 1 cut(s) 1303
BcnI CCSGG 1 cut(s) 1232
BcoDI GTCTC 1 cut(s) 1351
BfaI CTAG 4 cut(s) 176, 284, 516, 1100
BglII AGATCT 1 cut(s) 23
BisI GCNGC 2 cut(s) 225, 248
BlsI GCNGC 2 cut(s) 226, 249
Bme1390I CCNGG 5 cut(s) 34, 190, 433, 608, 1232
Bme18I GGWCC 1 cut(s) 602
BmgT120I GGNCC 1 cut(s) 602
BmiI GGNNCC 3 cut(s) 603, 604, 909
BmrFI CCNGG 5 cut(s) 34, 190, 433, 608, 1232
BmsI GCATC 4 cut(s) 277, 538, 963, 984
BpuEI CTTGAG 2 cut(s) 560, 601
BpuMI CCSGG 1 cut(s) 1232
BsaI GGTCTC 1 cut(s) 1351
BsaJI CCNNGG 4 cut(s) 431, 432, 606, 607
BsaXI ACNNNNNCTCC 2 cut(s) 163, 193
Bsc4I CCNNNNNNNGG 4 cut(s) 521, 945, 1269, 1423
Bse1I ACTGG 4 cut(s) 408, 468, 904, 1147
BseBI CCWGG 4 cut(s) 34, 190, 433, 608
BseDI CCNNGG 4 cut(s) 431, 432, 606, 607
BseGI GGATG 2 cut(s) 268, 787
BseLI CCNNNNNNNGG 4 cut(s) 521, 945, 1269, 1423
BseNI ACTGG 4 cut(s) 408, 468, 904, 1147
BseRI GAGGAG 3 cut(s) 107, 115, 1182
BseSI GKGCMC 1 cut(s) 1237
BseXI GCAGC 2 cut(s) 234, 236
Bsh1236I CGCG 1 cut(s) 119
BshFI GGCC 2 cut(s) 721, 1158
BshNI GGYRCC 1 cut(s) 907
BsiHKAI GWGCWC 2 cut(s) 144, 1402
BsiSI CCGG 2 cut(s) 89, 1231
BslFI GGGAC 3 cut(s) 615, 828, 1088
BslI CCNNNNNNNGG 4 cut(s) 521, 945, 1269, 1423
BsmAI GTCTC 1 cut(s) 1351
BsmFI GGGAC 3 cut(s) 615, 828, 1088
BsnI GGCC 2 cut(s) 721, 1158
Bso31I GGTCTC 1 cut(s) 1351
Bsp1286I GDGCHC 3 cut(s) 144, 1237, 1402
Bsp143I GATC 3 cut(s) 23, 988, 1303
BspACI CCGC 2 cut(s) 43, 1433
BspANI GGCC 2 cut(s) 721, 1158
BspFNI CGCG 1 cut(s) 119
BspLI GGNNCC 3 cut(s) 603, 604, 909
BspPI GGATC 1 cut(s) 983
BspT107I GGYRCC 1 cut(s) 907
BspTNI GGTCTC 1 cut(s) 1351
BsrI ACTGG 4 cut(s) 408, 468, 904, 1147
BssECI CCNNGG 4 cut(s) 431, 432, 606, 607
BssMI GATC 3 cut(s) 23, 988, 1303
Bst2UI CCWGG 4 cut(s) 34, 190, 433, 608
Bst4CI ACNGT 3 cut(s) 71, 1134, 1388
Bst6I CTCTTC 1 cut(s) 1200
BstC8I GCNNGC 1 cut(s) 365
BstDEI CTNAG 2 cut(s) 713, 1310
BstF5I GGATG 2 cut(s) 268, 787
BstFNI CGCG 1 cut(s) 119
BstHHI GCGC 3 cut(s) 119, 894, 1023
BstKTI GATC 3 cut(s) 26, 991, 1306
BstMAI GTCTC 1 cut(s) 1351
BstMBI GATC 3 cut(s) 23, 988, 1303
BstMWI GCNNNNNNNGC 2 cut(s) 159, 1417
BstNI CCWGG 4 cut(s) 34, 190, 433, 608
BstNSI RCATGY 2 cut(s) 821, 1378
BstSCI CCNGG 5 cut(s) 32, 188, 431, 606, 1230
BstSLI GKGCMC 1 cut(s) 1237
BstUI CGCG 1 cut(s) 119
BstV1I GCAGC 2 cut(s) 234, 236
BstX2I RGATCY 1 cut(s) 23
BstXI CCANNNNNNTGG 1 cut(s) 1154
BstYI RGATCY 1 cut(s) 23
BsuRI GGCC 2 cut(s) 721, 1158
BtgZI GCGATG 1 cut(s) 911
BtsCI GGATG 2 cut(s) 268, 787
BtsIMutI CAGTG 1 cut(s) 461
Cac8I GCNNGC 1 cut(s) 365
CfoI GCGC 3 cut(s) 119, 894, 1023
Cfr13I GGNCC 1 cut(s) 602
CseI GACGC 2 cut(s) 356, 1085
Csp6I GTAC 3 cut(s) 508, 1082, 1108
CviAII CATG 5 cut(s) 47, 658, 818, 945, 1375
CviQI GTAC 3 cut(s) 508, 1082, 1108
DdeI CTNAG 2 cut(s) 713, 1310
DpnI GATC 3 cut(s) 25, 990, 1305
DpnII GATC 3 cut(s) 23, 988, 1303
Eam1104I CTCTTC 1 cut(s) 1200
EarI CTCTTC 1 cut(s) 1200
Eco147I AGGCCT 1 cut(s) 721
Eco31I GGTCTC 1 cut(s) 1351
Eco47I GGWCC 1 cut(s) 602
Eco57I CTGAAG 2 cut(s) 1161, 1233
EcoO109I RGGNCCY 1 cut(s) 602
EcoRII CCWGG 4 cut(s) 32, 188, 431, 606
EcoT22I ATGCAT 1 cut(s) 270
FaeI CATG 5 cut(s) 50, 661, 821, 948, 1378
FaqI GGGAC 3 cut(s) 615, 828, 1088
FatI CATG 5 cut(s) 46, 657, 817, 944, 1374
FauI CCCGC 1 cut(s) 50
FauNDI CATATG 1 cut(s) 880
FbaI TGATCA 1 cut(s) 1303
Fnu4HI GCNGC 2 cut(s) 225, 248
FokI GGATG 2 cut(s) 255, 794
Fsp4HI GCNGC 2 cut(s) 225, 248
FspBI CTAG 4 cut(s) 176, 284, 516, 1100
FspI TGCGCA 1 cut(s) 893
GlaI GCGC 3 cut(s) 118, 893, 1022
GluI GCNGC 2 cut(s) 225, 248
HaeIII GGCC 2 cut(s) 721, 1158
HapII CCGG 2 cut(s) 89, 1231
HgaI GACGC 2 cut(s) 356, 1085
HhaI GCGC 3 cut(s) 119, 894, 1023
Hin1II CATG 5 cut(s) 50, 661, 821, 948, 1378
Hin6I GCGC 3 cut(s) 117, 892, 1021
HinP1I GCGC 3 cut(s) 117, 892, 1021
HincII GTYRAC 1 cut(s) 1285
HindII GTYRAC 1 cut(s) 1285
HindIII AAGCTT 1 cut(s) 1312
HinfI GANTC 5 cut(s) 77, 183, 407, 612, 978
HpaI GTTAAC 1 cut(s) 1285
HpaII CCGG 2 cut(s) 89, 1231
HphI GGTGA 3 cut(s) 149, 311, 1153
Hpy166II GTNNAC 5 cut(s) 319, 1137, 1285, 1293, 1391
Hpy188I TCNGA 8 cut(s) 30, 534, 698, 706, 993, 1033, 1153, 1180
Hpy188III TCNNGA 5 cut(s) 442, 624, 824, 1100, 1211
Hpy8I GTNNAC 5 cut(s) 319, 1137, 1285, 1293, 1391
Hpy99I CGWCG 3 cut(s) 71, 257, 372
HpyAV CCTTC 7 cut(s) 162, 281, 501, 568, 1185, 1208, 1446
HpyCH4III ACNGT 3 cut(s) 71, 1134, 1388
HpyCH4IV ACGT 3 cut(s) 639, 1206, 1393
HpyCH4V TGCA 4 cut(s) 224, 268, 583, 690
HpyF10VI GCNNNNNNNGC 2 cut(s) 159, 1417
HpyF3I CTNAG 2 cut(s) 713, 1310
HpySE526I ACGT 3 cut(s) 639, 1206, 1393
Hsp92II CATG 5 cut(s) 50, 661, 821, 948, 1378
HspAI GCGC 3 cut(s) 117, 892, 1021
KflI GGGWCCC 1 cut(s) 602
Ksp22I TGATCA 1 cut(s) 1303
KspAI GTTAAC 1 cut(s) 1285
Kzo9I GATC 3 cut(s) 23, 988, 1303
LmnI GCTCC 1 cut(s) 62
Lsp1109I GCAGC 2 cut(s) 234, 236
LweI GCATC 4 cut(s) 277, 538, 963, 984
MaeI CTAG 4 cut(s) 176, 284, 516, 1100
MaeII ACGT 3 cut(s) 639, 1206, 1393
MaeIII GTNAC 2 cut(s) 399, 1202
MalI GATC 3 cut(s) 25, 990, 1305
MboI GATC 3 cut(s) 23, 988, 1303
MboII GAAGA 4 cut(s) 675, 880, 1187, 1353
MflI RGATCY 1 cut(s) 23
MhlI GDGCHC 3 cut(s) 144, 1237, 1402
MluCI AATT 8 cut(s) 197, 327, 840, 871, 940, 1245, 1252, 1334
MlyI GAGTC 1 cut(s) 972
MmeI TCCRAC 3 cut(s) 53, 325, 1071
Mph1103I ATGCAT 1 cut(s) 270
MroXI GAANNNNTTC 2 cut(s) 197, 1412
MseI TTAA 3 cut(s) 642, 1275, 1284
MspI CCGG 2 cut(s) 89, 1231
MspR9I CCNGG 5 cut(s) 34, 190, 433, 608, 1232
MvaI CCWGG 4 cut(s) 34, 190, 433, 608
MvnI CGCG 1 cut(s) 119
MwoI GCNNNNNNNGC 2 cut(s) 159, 1417
NciI CCSGG 1 cut(s) 1232
NdeI CATATG 1 cut(s) 880
NdeII GATC 3 cut(s) 23, 988, 1303
NlaIII CATG 5 cut(s) 50, 661, 821, 948, 1378
NlaIV GGNNCC 3 cut(s) 603, 604, 909
NsbI TGCGCA 1 cut(s) 893
NsiI ATGCAT 1 cut(s) 270
NspI RCATGY 2 cut(s) 821, 1378
PasI CCCWGGG 2 cut(s) 432, 607
PceI AGGCCT 1 cut(s) 721
PciI ACATGT 2 cut(s) 817, 1374
PcsI WCGNNNNNNNCGW 1 cut(s) 72
PdmI GAANNNNTTC 2 cut(s) 197, 1412
PfeI GAWTC 4 cut(s) 77, 183, 407, 612
PflMI CCANNNNNTGG 1 cut(s) 945
PkrI GCNGC 2 cut(s) 226, 249
PleI GAGTC 1 cut(s) 972
PpsI GAGTC 1 cut(s) 972
PpuMI RGGWCCY 1 cut(s) 602
PscI ACATGT 2 cut(s) 817, 1374
Psp5II RGGWCCY 1 cut(s) 602
Psp6I CCWGG 4 cut(s) 32, 188, 431, 606
PspGI CCWGG 4 cut(s) 32, 188, 431, 606
PspN4I GGNNCC 3 cut(s) 603, 604, 909
PspPI GGNCC 1 cut(s) 602
PspPPI RGGWCCY 1 cut(s) 602
PsuI RGATCY 1 cut(s) 23
RsaI GTAC 3 cut(s) 509, 1083, 1109
RsaNI GTAC 3 cut(s) 508, 1082, 1108
SaqAI TTAA 3 cut(s) 642, 1275, 1284
SatI GCNGC 2 cut(s) 225, 248
Sau3AI GATC 3 cut(s) 23, 988, 1303
Sau96I GGNCC 1 cut(s) 602
SchI GAGTC 1 cut(s) 972
ScrFI CCNGG 5 cut(s) 34, 190, 433, 608, 1232
SduI GDGCHC 3 cut(s) 144, 1237, 1402
SfaNI GCATC 4 cut(s) 277, 538, 963, 984
SinI GGWCC 1 cut(s) 602
SmlI CTYRAG 2 cut(s) 539, 616
SmoI CTYRAG 2 cut(s) 539, 616
Sse9I AATT 8 cut(s) 197, 327, 840, 871, 940, 1245, 1252, 1334
SseBI AGGCCT 1 cut(s) 721
SsiI CCGC 2 cut(s) 43, 1433
SspI AATATT 1 cut(s) 746
SspMI CTAG 4 cut(s) 176, 284, 516, 1100
StuI AGGCCT 1 cut(s) 721
StyD4I CCNGG 5 cut(s) 32, 188, 431, 606, 1230
TaaI ACNGT 3 cut(s) 71, 1134, 1388
TaiI ACGT 3 cut(s) 642, 1209, 1396
TaqI TCGA 1 cut(s) 181
TasI AATT 8 cut(s) 197, 327, 840, 871, 940, 1245, 1252, 1334
TatI WGTACW 1 cut(s) 1107
TfiI GAWTC 4 cut(s) 77, 183, 407, 612
Tru1I TTAA 3 cut(s) 642, 1275, 1284
Tru9I TTAA 3 cut(s) 642, 1275, 1284
TscAI CASTG 1 cut(s) 468
TseI GCWGC 2 cut(s) 224, 247
TspDTI ATGAA 7 cut(s) 63, 674, 933, 1143, 1209, 1293, 1361
TspRI CASTG 1 cut(s) 468
Van91I CCANNNNNTGG 1 cut(s) 945
VpaK11BI GGWCC 1 cut(s) 602
XapI RAATTY 4 cut(s) 197, 327, 871, 1252
XbaI TCTAGA 1 cut(s) 1099
XceI RCATGY 2 cut(s) 821, 1378
XmnI GAANNNNTTC 2 cut(s) 197, 1412
XspI CTAG 4 cut(s) 176, 284, 516, 1100
Zsp2I ATGCAT 1 cut(s) 270
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.