pycom16g06870

Putative small multi-drug export protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0011202
Physical Location & Seq
Reverse (-)
4461391 .. 4462840
1450 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 960 bp
ATGGCTGCATCTCTATCAGCATCCCCATCGCCATTGACGTCATTTTTGTCTCTCAGAAAAACCCACTTCGTATTTTCACCAAAAAATTGCCGCCCAATCATTAACCGCAACAAACAATCCCCCTTCAGCCTCAATGCAGACTTTAAGTTTCAAACTTTGCCCCAATCAAATCCCTTTTTAGCAATTTCACCCCTCGCTCGCGCTGCAACGGGAGTTTCCTCACGTGGGTTTCCTGAAACAGACGAGGAAGATGAGCTTATGCCGTTGTTTGAGGAGCGGCCGGTCAAATTTGCCTTCTGGGTTTTGATATGGGCATCCGTGTCGCTTGCCTGGTTCGCGGCTTCCGGGGATGCAAATGCTGCGGCTGATTCCATTAGAGCCTCCAACTTTGGCTTGAAGATTGCGACGGTGCTTCGAAGCTCCGGCTGGCCTGATGAGGCTGTGGTGTTTGCTCTTGCCACACTTCCGGTCATTGAGCTTCGTGGGGCTATTCCTGTTGGTTACTGGTTGCAACTTAAGCCTGTTCTGCTAACAGTTCTATCTGTTCTTGGGAACATGGTTCCTGTGCCCTTCATCATACTTTACTTGAAGAGGTTTGCATCGTTCCTTGCTGGAAAGAACAAGGCCGCAGCTCAGTTCCTTGACTTGCTATTTGTGAGGGCTAGAGAGAAGGCTGGCCCCGTAGAGGAGTTCCAGTGGATTGGTCTGATGCTTTTTGTGGCTGTGCCTTTCCCCGGAACAGGAGCATGGACAGGGGCCTTCATAGCTTCGATTCTCGACATGCCATTCTGGTCAGCATTGTCTGCAAATTTCTTTGGTGTTGTACTGGCCGGGCTTCTGGTAAATTTGCTGGTGAACCTTGGCCTCAAATACGCAATTGTTGCTGGTATTGTCCTCTTCTTTATTTCCACATTCATGTGGACCATCCTTCGCAATCTTAAGAAGTCTTTTCGCTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Sm_multidrug_ex

Protein Analysis

320

Amino Acids

34.96

Weight (kDa)

9.85

Isoelectric Point (pI)

46.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Sm_multidrug_ex PF06695 159 - 279 2.9e-37 Putative small multi-drug export protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011202)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G02590
fragaria_vesca FvH4_4g32070
malus_domestica MD13G1079600.v1.1 MD16G1079500.v1.1
prunus_persica Prupe.1G267200_v2.0.a1
pyrus_communis pycom13g06930 pycom16g06870
rosa_chinensis RchiOBHm_Chr4g0440761
rosa_laevigata RLG00000006156
rosa_multiflora Rmu_co8415195.1_g000001 Rmu_sc0000424.1_g000005 Rmu_sc0022158.1_g000003
rosa_roxburghii Rroxscaffold_5G00381520
rosa_rugosa Rorug04G0327700
rosa_samantha Rh4AG379500 Rh4BG392400 Rh4CG407100 Rh4DG386200
rosa_wichuraiana Rw4G032640 Rw4G032670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 41
AccBSI CCGCTC 1 cut(s) 277
AccII CGCG 2 cut(s) 201, 338
AciI CCGC 6 cut(s) 91, 106, 277, 338, 362, 627
AcoI YGGCCR 2 cut(s) 278, 828
AcsI RAATTY 3 cut(s) 287, 808, 844
AcuI CTGAAG 1 cut(s) 109
AcvI CACGTG 1 cut(s) 224
AcyI GRCGYC 1 cut(s) 38
AfaI GTAC 1 cut(s) 825
AfiI CCNNNNNNNGG 4 cut(s) 225, 685, 734, 740
AflII CTTAAG 2 cut(s) 515, 938
AgsI TTSAA 3 cut(s) 152, 397, 589
AjnI CCWGG 1 cut(s) 329
AluBI AGCT 5 cut(s) 256, 420, 478, 632, 767
AluI AGCT 5 cut(s) 256, 420, 478, 632, 767
Alw26I GTCTC 1 cut(s) 54
AoxI GGCC 7 cut(s) 278, 428, 624, 676, 756, 828, 862
ApeKI GCWGC 4 cut(s) 5, 203, 359, 629
ApoI RAATTY 3 cut(s) 287, 808, 844
ArsI GACNNNNNNTTYG 4 cut(s) 28, 60, 635, 667
AspLEI GCGC 1 cut(s) 203
AspS9I GGNCC 3 cut(s) 677, 756, 921
AsuC2I CCSGG 3 cut(s) 346, 735, 832
AsuHPI GGTGA 3 cut(s) 69, 180, 865
AsuII TTCGAA 1 cut(s) 415
AvaII GGWCC 1 cut(s) 921
BaeGI GKGCMC 1 cut(s) 570
BbrPI CACGTG 1 cut(s) 224
BbvI GCAGC 3 cut(s) 190, 346, 641
BccI CCATC 2 cut(s) 34, 932
BceAI ACGGC 1 cut(s) 247
BcgI CGANNNNNNTGC 4 cut(s) 9, 43, 303, 337
BciT130I CCWGG 1 cut(s) 331
BcnI CCSGG 3 cut(s) 346, 735, 832
BcoDI GTCTC 1 cut(s) 54
BfaI CTAG 1 cut(s) 663
BfrI CTTAAG 2 cut(s) 515, 938
BisI GCNGC 9 cut(s) 6, 91, 204, 278, 339, 360, 363, 627, 630
BlsI GCNGC 9 cut(s) 7, 92, 205, 279, 340, 361, 364, 628, 631
Bme1390I CCNGG 4 cut(s) 331, 346, 735, 832
Bme18I GGWCC 1 cut(s) 921
BmgT120I GGNCC 3 cut(s) 677, 756, 921
BmiI GGNNCC 3 cut(s) 561, 679, 757
BmrFI CCNGG 4 cut(s) 331, 346, 735, 832
BmsI GCATC 6 cut(s) 17, 29, 323, 340, 608, 699
Bpu14I TTCGAA 1 cut(s) 415
BpuMI CCSGG 3 cut(s) 346, 735, 832
BsaAI YACGTR 1 cut(s) 224
BsaHI GRCGYC 1 cut(s) 38
BsaJI CCNNGG 3 cut(s) 345, 733, 859
BsaWI WCCGGW 1 cut(s) 466
BsaXI ACNNNNNCTCC 2 cut(s) 680, 710
Bsc4I CCNNNNNNNGG 4 cut(s) 225, 685, 734, 740
Bse118I RCCGGY 1 cut(s) 280
Bse1I ACTGG 3 cut(s) 509, 694, 831
BseBI CCWGG 1 cut(s) 331
BseDI CCNNGG 3 cut(s) 345, 733, 859
BseGI GGATG 4 cut(s) 20, 314, 355, 924
BseLI CCNNNNNNNGG 4 cut(s) 225, 685, 734, 740
BseMII CTCAG 2 cut(s) 67, 647
BseNI ACTGG 3 cut(s) 509, 694, 831
BseRI GAGGAG 2 cut(s) 287, 701
BseSI GKGCMC 1 cut(s) 570
BseX3I CGGCCG 1 cut(s) 278
BseXI GCAGC 3 cut(s) 190, 346, 641
Bsh1236I CGCG 2 cut(s) 201, 338
Bsh1285I CGRYCG 1 cut(s) 281
BshFI GGCC 7 cut(s) 280, 430, 626, 678, 758, 830, 864
BsiEI CGRYCG 1 cut(s) 281
BsiSI CCGG 6 cut(s) 281, 345, 423, 467, 735, 831
BslI CCNNNNNNNGG 4 cut(s) 225, 685, 734, 740
BsmAI GTCTC 1 cut(s) 54
BsnI GGCC 7 cut(s) 280, 430, 626, 678, 758, 830, 864
Bsp119I TTCGAA 1 cut(s) 415
Bsp1286I GDGCHC 1 cut(s) 570
BspACI CCGC 6 cut(s) 91, 106, 277, 338, 362, 627
BspANI GGCC 7 cut(s) 280, 430, 626, 678, 758, 830, 864
BspCNI CTCAG 2 cut(s) 66, 646
BspFNI CGCG 2 cut(s) 201, 338
BspHI TCATGA 1 cut(s) 956
BspLI GGNNCC 3 cut(s) 561, 679, 757
BspT104I TTCGAA 1 cut(s) 415
BspTI CTTAAG 2 cut(s) 515, 938
BsrBI CCGCTC 1 cut(s) 277
BsrFI RCCGGY 1 cut(s) 280
BsrI ACTGG 3 cut(s) 509, 694, 831
BssAI RCCGGY 1 cut(s) 280
BssECI CCNNGG 3 cut(s) 345, 733, 859
BssNI GRCGYC 1 cut(s) 38
BssT1I CCWWGG 1 cut(s) 859
Bst2UI CCWGG 1 cut(s) 331
Bst4CI ACNGT 2 cut(s) 409, 535
Bst6I CTCTTC 2 cut(s) 584, 902
BstACI GRCGYC 1 cut(s) 38
BstAFI CTTAAG 2 cut(s) 515, 938
BstAPI GCANNNNNTGC 3 cut(s) 359, 803, 881
BstBAI YACGTR 1 cut(s) 224
BstBI TTCGAA 1 cut(s) 415
BstC8I GCNNGC 4 cut(s) 199, 327, 428, 676
BstDEI CTNAG 2 cut(s) 53, 633
BstF5I GGATG 4 cut(s) 20, 314, 355, 924
BstFNI CGCG 2 cut(s) 201, 338
BstHHI GCGC 1 cut(s) 203
BstMAI GTCTC 1 cut(s) 54
BstMCI CGRYCG 1 cut(s) 281
BstMWI GCNNNNNNNGC 8 cut(s) 203, 335, 359, 517, 526, 764, 803, 881
BstNI CCWGG 1 cut(s) 331
BstNSI RCATGY 1 cut(s) 784
BstSCI CCNGG 4 cut(s) 329, 344, 733, 830
BstSLI GKGCMC 1 cut(s) 570
BstUI CGCG 2 cut(s) 201, 338
BstV1I GCAGC 3 cut(s) 190, 346, 641
BstXI CCANNNNNNTGG 1 cut(s) 701
BstZI CGGCCG 1 cut(s) 278
BsuRI GGCC 7 cut(s) 280, 430, 626, 678, 758, 830, 864
BtgZI GCGATG 1 cut(s) 12
BtsCI GGATG 4 cut(s) 20, 314, 355, 924
BtsIMutI CAGTG 1 cut(s) 701
Cac8I GCNNGC 4 cut(s) 199, 327, 428, 676
CciI TCATGA 1 cut(s) 956
CfoI GCGC 1 cut(s) 203
Cfr10I RCCGGY 1 cut(s) 280
Cfr13I GGNCC 3 cut(s) 677, 756, 921
Csp6I GTAC 1 cut(s) 824
CviAII CATG 5 cut(s) 556, 747, 781, 916, 957
CviQI GTAC 1 cut(s) 824
DdeI CTNAG 2 cut(s) 53, 633
EaeI YGGCCR 2 cut(s) 278, 828
EagI CGGCCG 1 cut(s) 278
Eam1104I CTCTTC 2 cut(s) 584, 902
EarI CTCTTC 2 cut(s) 584, 902
EclXI CGGCCG 1 cut(s) 278
Eco130I CCWWGG 1 cut(s) 859
Eco47I GGWCC 1 cut(s) 921
Eco52I CGGCCG 1 cut(s) 278
Eco57I CTGAAG 1 cut(s) 109
Eco72I CACGTG 1 cut(s) 224
EcoO109I RGGNCCY 1 cut(s) 756
EcoRII CCWGG 1 cut(s) 329
EcoT14I CCWWGG 1 cut(s) 859
ErhI CCWWGG 1 cut(s) 859
FaeI CATG 5 cut(s) 559, 750, 784, 919, 960
FaiI YATR 9 cut(s) 260, 310, 557, 578, 748, 764, 782, 917, 958
FalI AAGNNNNNCTT 2 cut(s) 240, 272
FatI CATG 5 cut(s) 555, 746, 780, 915, 956
Fnu4HI GCNGC 9 cut(s) 6, 91, 204, 278, 339, 360, 363, 627, 630
FokI GGATG 4 cut(s) 7, 301, 362, 911
Fsp4HI GCNGC 9 cut(s) 6, 91, 204, 278, 339, 360, 363, 627, 630
FspBI CTAG 1 cut(s) 663
GlaI GCGC 1 cut(s) 202
GluI GCNGC 9 cut(s) 6, 91, 204, 278, 339, 360, 363, 627, 630
HaeIII GGCC 7 cut(s) 280, 430, 626, 678, 758, 830, 864
HapII CCGG 6 cut(s) 281, 345, 423, 467, 735, 831
HhaI GCGC 1 cut(s) 203
Hin1I GRCGYC 1 cut(s) 38
Hin1II CATG 5 cut(s) 559, 750, 784, 919, 960
Hin6I GCGC 1 cut(s) 201
HinP1I GCGC 1 cut(s) 201
HinfI GANTC 2 cut(s) 368, 772
HpaII CCGG 6 cut(s) 281, 345, 423, 467, 735, 831
HphI GGTGA 3 cut(s) 69, 180, 865
Hpy166II GTNNAC 2 cut(s) 856, 921
Hpy188I TCNGA 2 cut(s) 56, 708
Hpy188III TCNNGA 3 cut(s) 233, 776, 957
Hpy8I GTNNAC 2 cut(s) 856, 921
Hpy99I CGWCG 1 cut(s) 409
HpyAV CCTTC 6 cut(s) 133, 304, 580, 664, 769, 938
HpyCH4III ACNGT 2 cut(s) 409, 535
HpyCH4IV ACGT 2 cut(s) 38, 223
HpyCH4V TGCA 7 cut(s) 8, 137, 206, 353, 511, 599, 806
HpyF10VI GCNNNNNNNGC 8 cut(s) 203, 335, 359, 517, 526, 764, 803, 881
HpyF3I CTNAG 2 cut(s) 53, 633
HpySE526I ACGT 2 cut(s) 38, 223
Hsp92I GRCGYC 1 cut(s) 38
Hsp92II CATG 5 cut(s) 559, 750, 784, 919, 960
HspAI GCGC 1 cut(s) 201
LmnI GCTCC 3 cut(s) 274, 425, 743
Lsp1109I GCAGC 3 cut(s) 190, 346, 641
LweI GCATC 6 cut(s) 17, 29, 323, 340, 608, 699
MaeI CTAG 1 cut(s) 663
MaeII ACGT 2 cut(s) 38, 223
MaeIII GTNAC 1 cut(s) 500
MbiI CCGCTC 1 cut(s) 277
MboII GAAGA 4 cut(s) 260, 409, 601, 889
MfeI CAATTG 1 cut(s) 876
MhlI GDGCHC 1 cut(s) 570
MluCI AATT 6 cut(s) 85, 183, 287, 808, 844, 876
MmeI TCCRAC 1 cut(s) 408
MseI TTAA 4 cut(s) 102, 144, 516, 939
MslI CAYNNNNRTG 2 cut(s) 914, 916
MspCI CTTAAG 2 cut(s) 515, 938
MspI CCGG 6 cut(s) 281, 345, 423, 467, 735, 831
MspR9I CCNGG 4 cut(s) 331, 346, 735, 832
MunI CAATTG 1 cut(s) 876
MvaI CCWGG 1 cut(s) 331
MvnI CGCG 2 cut(s) 201, 338
MwoI GCNNNNNNNGC 8 cut(s) 203, 335, 359, 517, 526, 764, 803, 881
NciI CCSGG 3 cut(s) 346, 735, 832
NlaIII CATG 5 cut(s) 559, 750, 784, 919, 960
NlaIV GGNNCC 3 cut(s) 561, 679, 757
NspI RCATGY 1 cut(s) 784
NspV TTCGAA 1 cut(s) 415
PagI TCATGA 1 cut(s) 956
PcsI WCGNNNNNNNCGW 1 cut(s) 35
PfeI GAWTC 2 cut(s) 368, 772
PkrI GCNGC 9 cut(s) 7, 92, 205, 279, 340, 361, 364, 628, 631
PmaCI CACGTG 1 cut(s) 224
PmlI CACGTG 1 cut(s) 224
Ppu21I YACGTR 1 cut(s) 224
Psp6I CCWGG 1 cut(s) 329
PspCI CACGTG 1 cut(s) 224
PspGI CCWGG 1 cut(s) 329
PspN4I GGNNCC 3 cut(s) 561, 679, 757
PspPI GGNCC 3 cut(s) 677, 756, 921
RsaI GTAC 1 cut(s) 825
RsaNI GTAC 1 cut(s) 824
RseI CAYNNNNRTG 2 cut(s) 914, 916
SaqAI TTAA 4 cut(s) 102, 144, 516, 939
SatI GCNGC 9 cut(s) 6, 91, 204, 278, 339, 360, 363, 627, 630
Sau96I GGNCC 3 cut(s) 677, 756, 921
ScrFI CCNGG 4 cut(s) 331, 346, 735, 832
SduI GDGCHC 1 cut(s) 570
SetI ASST 9 cut(s) 41, 226, 258, 422, 480, 596, 634, 769, 861
SfaNI GCATC 6 cut(s) 17, 29, 323, 340, 608, 699
SfuI TTCGAA 1 cut(s) 415
SinI GGWCC 1 cut(s) 921
SmiMI CAYNNNNRTG 2 cut(s) 914, 916
SmlI CTYRAG 2 cut(s) 515, 938
SmoI CTYRAG 2 cut(s) 515, 938
Sse9I AATT 6 cut(s) 85, 183, 287, 808, 844, 876
SsiI CCGC 6 cut(s) 91, 106, 277, 338, 362, 627
SspMI CTAG 1 cut(s) 663
StyD4I CCNGG 4 cut(s) 329, 344, 733, 830
StyI CCWWGG 1 cut(s) 859
TaaI ACNGT 2 cut(s) 409, 535
TaiI ACGT 2 cut(s) 41, 226
TaqI TCGA 3 cut(s) 415, 770, 777
TasI AATT 6 cut(s) 85, 183, 287, 808, 844, 876
TatI WGTACW 1 cut(s) 823
TauI GCSGC 5 cut(s) 93, 280, 341, 365, 629
TfiI GAWTC 2 cut(s) 368, 772
Tru1I TTAA 4 cut(s) 102, 144, 516, 939
Tru9I TTAA 4 cut(s) 102, 144, 516, 939
TscAI CASTG 1 cut(s) 701
TseI GCWGC 4 cut(s) 5, 203, 359, 629
TspDTI ATGAA 3 cut(s) 562, 751, 904
TspGWI ACGGA 1 cut(s) 307
TspRI CASTG 1 cut(s) 701
Vha464I CTTAAG 2 cut(s) 515, 938
VpaK11BI GGWCC 1 cut(s) 921
XapI RAATTY 3 cut(s) 287, 808, 844
XceI RCATGY 1 cut(s) 784
XspI CTAG 1 cut(s) 663
ZraI GACGTC 1 cut(s) 39
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.