pycom16g07170

5-formyltetrahydrofolate cyclo-ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0005536
Physical Location & Seq
Reverse (-)
4677391 .. 4679448
2058 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1035 bp
ATGGTACTATTCAATTTACCTTTTATTTTATCCTTATCATTAAAATTCAAAGTTTTCAAACATATCCGACCCGAACTCTTCAACCGCGCCTACGCGCAACCAAAGAGCGCCAAATTAACGGAAAAACCCACAAAAGCACGAGAGCCACGAAGCATGTTCAGTCACAGCAGAGCAAGCGCGAGTGGGCTCATAAACGGGGCAAAGGAGGCGGTGGCGCTCATCACCCAGCCGCGCACGTTAGCTTCTCGTACCCTCTTCCCTTCTCGCTTAGCTAACGAACGCCTCGCATCCCATTCTCACCGCCCCTACGCCATCATGAACCGAGACGACAACGACCCAATCGAAGCTATATTCCGGCAGAAGCGGTTGCTCCGGTCCAGAATCCGCAAAGAGCTCAAGAATATGGACCCGGTTCGGAGGTCCGAAGAAGATGCTGCAATTCAGAGCATTGTTTTAAATGCTCCCTGGTTCCAATCCAGTAAGAGCATATGCGCTTACATAAGCTCTCCTGCTTTACGAGAAGTCGATACGTCAAGAATTGTTTCAGAAATTCTATCCAAACCTGCCAATGAGGGTGATGTGCAGAACAGGAAAAAGCTTTATGTTCCGCGTGTGGAGGATAGGAATAGCAACATGAGGATGCTTAGGATTTCCAGCGTTGATGATCTGATCGTGAACTCGATGGACATTTTGGAGCCAGCTCTTTTGGATTCTGATGGGAAACAACACGAAGATGTTTTGGAGGCAAGCGATCCAGTTGACTTGTTCATCCTACCCGGGCTTGCATTTGACAGATGTGGAAGCCGTTTAGGCCGTGGTGGAGGTTACTATGATTTGTTCCTAAAGAAGTACCAAGAGCTTACAAAGCAACGACAATGGAAGGAGCCCCTTCGAGTTGCGCTGTCATATTCTGTACAGATCGTGGAGGAAGGAGCCATAGCTGTCACTTCAAATGATGTTTCTGTGGATGCTCTTGCATCTCCAGCCGGTGTCATTCCCATAAGCCCCGCTGCTCAGGAGAGGAGCATGGGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00670 ko01100 map00670 map01100
Pfam Domains
Protein Families
5-FTHF_cyc-lig

Protein Analysis

345

Amino Acids

38.62

Weight (kDa)

9.55

Isoelectric Point (pI)

52.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
5-FTHF_cyc-lig PF01812 121 - 326 2e-29 5-formyltetrahydrofolate cyclo-ligase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 571
AccII CGCG 5 cut(s) 87, 95, 179, 232, 610
AciI CCGC 8 cut(s) 85, 209, 230, 301, 364, 385, 608, 1008
AclWI GGATC 1 cut(s) 746
AcsI RAATTY 2 cut(s) 44, 549
AfaI GTAC 4 cut(s) 6, 250, 851, 915
AgsI TTSAA 5 cut(s) 13, 49, 58, 82, 951
AjnI CCWGG 1 cut(s) 464
AluBI AGCT 9 cut(s) 242, 272, 347, 394, 504, 598, 701, 859, 941
AluI AGCT 9 cut(s) 242, 272, 347, 394, 504, 598, 701, 859, 941
Alw21I GWGCWC 1 cut(s) 396
Alw26I GTCTC 1 cut(s) 318
AlwI GGATC 1 cut(s) 746
Ama87I CYCGRG 1 cut(s) 776
AoxI GGCC 1 cut(s) 811
ApeKI GCWGC 2 cut(s) 434, 1010
ApoI RAATTY 2 cut(s) 44, 549
Asp700I GAANNNNTTC 1 cut(s) 541
AspLEI GCGC 8 cut(s) 89, 97, 110, 179, 217, 234, 494, 901
AspS9I GGNCC 3 cut(s) 375, 406, 420
AsuC2I CCSGG 3 cut(s) 410, 777, 778
AsuHPI GGTGA 3 cut(s) 214, 290, 587
AvaI CYCGRG 1 cut(s) 776
AvaII GGWCC 3 cut(s) 375, 406, 420
BanII GRGCYC 3 cut(s) 189, 396, 888
BauI CACGAG 1 cut(s) 138
Bbv12I GWGCWC 1 cut(s) 396
BbvI GCAGC 2 cut(s) 421, 997
BccI CCATC 3 cut(s) 320, 676, 710
BceAI ACGGC 2 cut(s) 789, 798
BcgI CGANNNNNNTGC 2 cut(s) 413, 447
BciT130I CCWGG 1 cut(s) 466
BcnI CCSGG 3 cut(s) 410, 777, 778
BcoDI GTCTC 1 cut(s) 318
BfoI RGCGCY 2 cut(s) 111, 218
BfuAI ACCTGC 1 cut(s) 571
BglI GCCNNNNNGGC 1 cut(s) 810
BisI GCNGC 3 cut(s) 230, 435, 1011
BlpI GCTNAGC 1 cut(s) 268
BlsI GCNGC 3 cut(s) 231, 436, 1012
Bme1390I CCNGG 4 cut(s) 410, 466, 777, 778
Bme18I GGWCC 3 cut(s) 375, 406, 420
BmeT110I CYCGRG 1 cut(s) 776
BmgT120I GGNCC 3 cut(s) 375, 406, 420
BmiI GGNNCC 5 cut(s) 408, 470, 696, 885, 934
BmrFI CCNGG 4 cut(s) 410, 466, 777, 778
BmsI GCATC 5 cut(s) 296, 421, 630, 958, 986
BpmI CTGGAG 1 cut(s) 966
Bpu10I CCTNAGC 2 cut(s) 644, 1014
Bpu1102I GCTNAGC 1 cut(s) 268
BpuEI CTTGAG 1 cut(s) 380
BpuMI CCSGG 3 cut(s) 410, 777, 778
BsaJI CCNNGG 3 cut(s) 464, 776, 814
BsaWI WCCGGW 1 cut(s) 372
Bse118I RCCGGY 1 cut(s) 986
Bse1I ACTGG 2 cut(s) 477, 755
BseBI CCWGG 1 cut(s) 466
BseDI CCNNGG 3 cut(s) 464, 776, 814
BseGI GGATG 4 cut(s) 287, 645, 768, 973
BseMII CTCAG 1 cut(s) 1028
BseNI ACTGG 2 cut(s) 477, 755
BseXI GCAGC 2 cut(s) 421, 997
BseYI CCCAGC 1 cut(s) 225
BsgI GTGCAG 1 cut(s) 602
Bsh1236I CGCG 5 cut(s) 87, 95, 179, 232, 610
BshFI GGCC 1 cut(s) 813
BsiHKAI GWGCWC 1 cut(s) 396
BsiHKCI CYCGRG 1 cut(s) 776
BsiSI CCGG 5 cut(s) 355, 373, 410, 777, 987
BsmAI GTCTC 1 cut(s) 318
BsmBI CGTCTC 1 cut(s) 318
BsnI GGCC 1 cut(s) 813
BsoBI CYCGRG 1 cut(s) 776
Bsp1286I GDGCHC 3 cut(s) 189, 396, 888
Bsp1407I TGTACA 1 cut(s) 913
Bsp143I GATC 4 cut(s) 664, 669, 751, 918
Bsp1720I GCTNAGC 1 cut(s) 268
BspACI CCGC 8 cut(s) 85, 209, 230, 301, 364, 385, 608, 1008
BspANI GGCC 1 cut(s) 813
BspCNI CTCAG 1 cut(s) 1027
BspFNI CGCG 5 cut(s) 87, 95, 179, 232, 610
BspHI TCATGA 1 cut(s) 315
BspLI GGNNCC 5 cut(s) 408, 470, 696, 885, 934
BspMI ACCTGC 1 cut(s) 571
BspPI GGATC 1 cut(s) 746
BsrFI RCCGGY 1 cut(s) 986
BsrGI TGTACA 1 cut(s) 913
BsrI ACTGG 2 cut(s) 477, 755
BssAI RCCGGY 1 cut(s) 986
BssECI CCNNGG 3 cut(s) 464, 776, 814
BssMI GATC 4 cut(s) 664, 669, 751, 918
BssSI CACGAG 1 cut(s) 138
Bst2BI CACGAG 1 cut(s) 138
Bst2UI CCWGG 1 cut(s) 466
Bst6I CTCTTC 2 cut(s) 83, 260
BstAUI TGTACA 1 cut(s) 913
BstC8I GCNNGC 4 cut(s) 175, 699, 748, 783
BstDEI CTNAG 3 cut(s) 268, 644, 1014
BstDSI CCRYGG 1 cut(s) 814
BstF5I GGATG 4 cut(s) 287, 645, 768, 973
BstFNI CGCG 5 cut(s) 87, 95, 179, 232, 610
BstH2I RGCGCY 2 cut(s) 111, 218
BstHHI GCGC 8 cut(s) 89, 97, 110, 179, 217, 234, 494, 901
BstKTI GATC 4 cut(s) 667, 672, 754, 921
BstMAI GTCTC 1 cut(s) 318
BstMBI GATC 4 cut(s) 664, 669, 751, 918
BstMWI GCNNNNNNNGC 5 cut(s) 174, 206, 810, 865, 983
BstNI CCWGG 1 cut(s) 466
BstNSI RCATGY 1 cut(s) 157
BstSCI CCNGG 4 cut(s) 408, 464, 775, 776
BstUI CGCG 5 cut(s) 87, 95, 179, 232, 610
BstV1I GCAGC 2 cut(s) 421, 997
BsuRI GGCC 1 cut(s) 813
BtgI CCRYGG 1 cut(s) 814
BtsCI GGATG 4 cut(s) 287, 645, 768, 973
BveI ACCTGC 1 cut(s) 571
Cac8I GCNNGC 4 cut(s) 175, 699, 748, 783
CciI TCATGA 1 cut(s) 315
CfoI GCGC 8 cut(s) 89, 97, 110, 179, 217, 234, 494, 901
Cfr10I RCCGGY 1 cut(s) 986
Cfr13I GGNCC 3 cut(s) 375, 406, 420
Cfr9I CCCGGG 1 cut(s) 776
Csp6I GTAC 4 cut(s) 5, 249, 850, 914
CviAII CATG 4 cut(s) 154, 316, 634, 1027
CviQI GTAC 4 cut(s) 5, 249, 850, 914
DdeI CTNAG 3 cut(s) 268, 644, 1014
DpnI GATC 4 cut(s) 666, 671, 753, 920
DpnII GATC 4 cut(s) 664, 669, 751, 918
DraI TTTAAA 1 cut(s) 456
Eam1104I CTCTTC 2 cut(s) 83, 260
EarI CTCTTC 2 cut(s) 83, 260
Ecl136II GAGCTC 1 cut(s) 394
Eco24I GRGCYC 3 cut(s) 189, 396, 888
Eco47I GGWCC 3 cut(s) 375, 406, 420
Eco53kI GAGCTC 1 cut(s) 394
Eco88I CYCGRG 1 cut(s) 776
EcoICRI GAGCTC 1 cut(s) 394
EcoRII CCWGG 1 cut(s) 464
EcoT38I GRGCYC 3 cut(s) 189, 396, 888
Esp3I CGTCTC 1 cut(s) 318
FaeI CATG 4 cut(s) 157, 319, 637, 1030
FatI CATG 4 cut(s) 153, 315, 633, 1026
FauI CCCGC 1 cut(s) 1015
FauNDI CATATG 1 cut(s) 488
Fnu4HI GCNGC 3 cut(s) 230, 435, 1011
FokI GGATG 4 cut(s) 274, 652, 755, 980
FriOI GRGCYC 3 cut(s) 189, 396, 888
Fsp4HI GCNGC 3 cut(s) 230, 435, 1011
GlaI GCGC 8 cut(s) 88, 96, 109, 178, 216, 233, 493, 900
GluI GCNGC 3 cut(s) 230, 435, 1011
GsaI CCCAGC 1 cut(s) 229
GsuI CTGGAG 1 cut(s) 966
HaeII RGCGCY 2 cut(s) 111, 218
HaeIII GGCC 1 cut(s) 813
HapII CCGG 5 cut(s) 355, 373, 410, 777, 987
HhaI GCGC 8 cut(s) 89, 97, 110, 179, 217, 234, 494, 901
Hin1II CATG 4 cut(s) 157, 319, 637, 1030
Hin6I GCGC 8 cut(s) 87, 95, 108, 177, 215, 232, 492, 899
HinP1I GCGC 8 cut(s) 87, 95, 108, 177, 215, 232, 492, 899
HincII GTYRAC 1 cut(s) 760
HindII GTYRAC 1 cut(s) 760
HindIII AAGCTT 1 cut(s) 596
HinfI GANTC 2 cut(s) 381, 710
HpaII CCGG 5 cut(s) 355, 373, 410, 777, 987
HphI GGTGA 3 cut(s) 214, 290, 587
Hpy166II GTNNAC 2 cut(s) 676, 760
Hpy188I TCNGA 7 cut(s) 68, 417, 424, 444, 547, 669, 715
Hpy188III TCNNGA 6 cut(s) 316, 378, 397, 534, 673, 1016
Hpy8I GTNNAC 2 cut(s) 676, 760
HpyAV CCTTC 4 cut(s) 270, 874, 899, 923
HpyCH4IV ACGT 2 cut(s) 236, 530
HpyCH4V TGCA 4 cut(s) 437, 583, 785, 977
HpyF10VI GCNNNNNNNGC 5 cut(s) 174, 206, 810, 865, 983
HpyF3I CTNAG 3 cut(s) 268, 644, 1014
HpySE526I ACGT 2 cut(s) 236, 530
Hsp92II CATG 4 cut(s) 157, 319, 637, 1030
HspAI GCGC 8 cut(s) 87, 95, 108, 177, 215, 232, 492, 899
Kzo9I GATC 4 cut(s) 664, 669, 751, 918
LmnI GCTCC 6 cut(s) 375, 466, 694, 883, 932, 1023
Lsp1109I GCAGC 2 cut(s) 421, 997
LweI GCATC 5 cut(s) 296, 421, 630, 958, 986
MaeII ACGT 2 cut(s) 236, 530
MaeIII GTNAC 3 cut(s) 161, 824, 943
MalI GATC 4 cut(s) 666, 671, 753, 920
MboI GATC 4 cut(s) 664, 669, 751, 918
MboII GAAGA 5 cut(s) 70, 247, 437, 440, 743
MhlI GDGCHC 3 cut(s) 189, 396, 888
MluCI AATT 6 cut(s) 13, 44, 113, 438, 537, 549
MmeI TCCRAC 1 cut(s) 91
MroXI GAANNNNTTC 1 cut(s) 541
MseI TTAA 3 cut(s) 41, 116, 455
MslI CAYNNNNRTG 2 cut(s) 638, 732
MspA1I CMGCKG 1 cut(s) 1010
MspI CCGG 5 cut(s) 355, 373, 410, 777, 987
MspR9I CCNGG 4 cut(s) 410, 466, 777, 778
MvaI CCWGG 1 cut(s) 466
MvnI CGCG 5 cut(s) 87, 95, 179, 232, 610
MwoI GCNNNNNNNGC 5 cut(s) 174, 206, 810, 865, 983
NciI CCSGG 3 cut(s) 410, 777, 778
NdeI CATATG 1 cut(s) 488
NdeII GATC 4 cut(s) 664, 669, 751, 918
NlaIII CATG 4 cut(s) 157, 319, 637, 1030
NlaIV GGNNCC 5 cut(s) 408, 470, 696, 885, 934
NmuCI GTSAC 2 cut(s) 161, 943
NspI RCATGY 1 cut(s) 157
PagI TCATGA 1 cut(s) 315
PcsI WCGNNNNNNNCGW 2 cut(s) 145, 339
PdmI GAANNNNTTC 1 cut(s) 541
PfeI GAWTC 2 cut(s) 381, 710
PkrI GCNGC 3 cut(s) 231, 436, 1012
Psp124BI GAGCTC 1 cut(s) 396
Psp6I CCWGG 1 cut(s) 464
PspFI CCCAGC 1 cut(s) 225
PspGI CCWGG 1 cut(s) 464
PspN4I GGNNCC 5 cut(s) 408, 470, 696, 885, 934
PspPI GGNCC 3 cut(s) 375, 406, 420
RsaI GTAC 4 cut(s) 6, 250, 851, 915
RsaNI GTAC 4 cut(s) 5, 249, 850, 914
RseI CAYNNNNRTG 2 cut(s) 638, 732
SacI GAGCTC 1 cut(s) 396
SaqAI TTAA 3 cut(s) 41, 116, 455
SatI GCNGC 3 cut(s) 230, 435, 1011
Sau3AI GATC 4 cut(s) 664, 669, 751, 918
Sau96I GGNCC 3 cut(s) 375, 406, 420
ScrFI CCNGG 4 cut(s) 410, 466, 777, 778
SduI GDGCHC 3 cut(s) 189, 396, 888
SfaNI GCATC 5 cut(s) 296, 421, 630, 958, 986
SinI GGWCC 3 cut(s) 375, 406, 420
SmaI CCCGGG 1 cut(s) 778
SmiMI CAYNNNNRTG 2 cut(s) 638, 732
SmlI CTYRAG 1 cut(s) 395
SmoI CTYRAG 1 cut(s) 395
Sse9I AATT 6 cut(s) 13, 44, 113, 438, 537, 549
SsiI CCGC 8 cut(s) 85, 209, 230, 301, 364, 385, 608, 1008
SstI GAGCTC 1 cut(s) 396
StyD4I CCNGG 4 cut(s) 408, 464, 775, 776
TaiI ACGT 2 cut(s) 239, 533
TaqI TCGA 4 cut(s) 342, 525, 680, 892
TasI AATT 6 cut(s) 13, 44, 113, 438, 537, 549
TatI WGTACW 1 cut(s) 913
TauI GCSGC 1 cut(s) 232
TfiI GAWTC 2 cut(s) 381, 710
Tru1I TTAA 3 cut(s) 41, 116, 455
Tru9I TTAA 3 cut(s) 41, 116, 455
TseFI GTSAC 2 cut(s) 161, 943
TseI GCWGC 2 cut(s) 434, 1010
Tsp45I GTSAC 2 cut(s) 161, 943
TspDTI ATGAA 2 cut(s) 332, 757
TspGWI ACGGA 1 cut(s) 134
TspMI CCCGGG 1 cut(s) 776
VpaK11BI GGWCC 3 cut(s) 375, 406, 420
XapI RAATTY 2 cut(s) 44, 549
XceI RCATGY 1 cut(s) 157
XmaI CCCGGG 1 cut(s) 776
XmnI GAANNNNTTC 1 cut(s) 541
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.