pycom16g07530

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0009900
Physical Location & Seq
Reverse (-)
4914100 .. 4914924
825 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 624 bp
ATGGGGAACTGTCAAGCCATTGATGCAGCAGCTCTGGTGATACAGCATCCGAGTGGGAAGATAGAGAGGATGTATTGTCCTATAAGTGCGAGTGAGGTGATGAGGTTGAACCCCGGTCACTACGTTTCCCTCATCATTCCATTGCCGGTTTCCGAACAAAATCACAAACAAGTAGCAGAGAATCACGAGGATGAGCACGGCAAAGCGGTGCGTTTCACTCGCATTAAGCTTCTCCGGCCGAACGAAACTCTCGCTCTCGGCCATGCCTACCGGCTGGTCACCACTCAAGAGGTTATGAAGGTTTTGAAGGCAAAAAAGTATGCGAAAACGAAGAAACAGCAGCTCGAAGTGCTCGAGAAGTTGCGAACGGAGCAAGGGAAGGAGAATTCAGGTTGTTCGGGCTGCGAAGAGGCAGCAGAAGCAACGAAATCTGATGAGAAGGAAAATATCAACCATGCTTCAAAACGCGAAAGGCACCGACCAAGGACATCATCAGCGAATGCTGCTGCTGCGGCTGCTGCTGCGCTGAGGTCGAAGTCTTGGCGCCCCTCGTTGCAGAGCATCTCCGAGGGCGTAACTGATCAGCGATTTCCAAACATCGAAAGCGAACAAAATGCAAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

208

Amino Acids

22.99

Weight (kDa)

9.26

Isoelectric Point (pI)

50.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PADRE PF14009 1 - 190 6.8e-27 PADRE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 474, 543
AccII CGCG 1 cut(s) 468
AciI CCGC 2 cut(s) 206, 512
AcoI YGGCCR 2 cut(s) 236, 259
AcsI RAATTY 1 cut(s) 385
AcyI GRCGYC 1 cut(s) 544
AgsI TTSAA 3 cut(s) 109, 307, 462
AluBI AGCT 3 cut(s) 32, 229, 343
AluI AGCT 3 cut(s) 32, 229, 343
Alw21I GWGCWC 2 cut(s) 198, 354
Ama87I CYCGRG 1 cut(s) 353
AoxI GGCC 2 cut(s) 236, 259
ApoI RAATTY 1 cut(s) 385
AspLEI GCGC 2 cut(s) 526, 546
AsuC2I CCSGG 1 cut(s) 114
AsuHPI GGTGA 3 cut(s) 49, 109, 271
AvaI CYCGRG 1 cut(s) 353
BanI GGYRCC 2 cut(s) 474, 543
BauI CACGAG 1 cut(s) 185
Bbv12I GWGCWC 2 cut(s) 198, 354
BbvCI CCTCAGC 1 cut(s) 527
BceAI ACGGC 1 cut(s) 214
BcgI CGANNNNNNTGC 1 cut(s) 596
BclI TGATCA 1 cut(s) 580
BcnI CCSGG 1 cut(s) 114
BfoI RGCGCY 1 cut(s) 547
Bme1390I CCNGG 1 cut(s) 114
BmeT110I CYCGRG 1 cut(s) 353
BmiI GGNNCC 2 cut(s) 476, 545
BmrFI CCNGG 1 cut(s) 114
BmsI GCATC 3 cut(s) 13, 55, 570
Bpu10I CCTNAGC 1 cut(s) 527
BpuEI CTTGAG 1 cut(s) 270
BpuMI CCSGG 1 cut(s) 114
BsaHI GRCGYC 1 cut(s) 544
BsaJI CCNNGG 3 cut(s) 112, 482, 567
Bse118I RCCGGY 2 cut(s) 145, 270
Bse3DI GCAATG 1 cut(s) 140
BseDI CCNNGG 3 cut(s) 112, 482, 567
BseGI GGATG 3 cut(s) 46, 75, 196
BseMI GCAATG 1 cut(s) 140
BseMII CTCAG 1 cut(s) 518
BseX3I CGGCCG 1 cut(s) 236
Bsh1236I CGCG 1 cut(s) 468
Bsh1285I CGRYCG 1 cut(s) 239
BshFI GGCC 2 cut(s) 238, 261
BshNI GGYRCC 2 cut(s) 474, 543
BsiEI CGRYCG 1 cut(s) 239
BsiHKAI GWGCWC 2 cut(s) 198, 354
BsiHKCI CYCGRG 1 cut(s) 353
BsiSI CCGG 4 cut(s) 114, 146, 235, 271
BsmI GAATGC 1 cut(s) 505
BsnI GGCC 2 cut(s) 238, 261
BsoBI CYCGRG 1 cut(s) 353
Bsp1286I GDGCHC 2 cut(s) 198, 354
Bsp143I GATC 1 cut(s) 580
BspACI CCGC 2 cut(s) 206, 512
BspANI GGCC 2 cut(s) 238, 261
BspCNI CTCAG 1 cut(s) 519
BspFNI CGCG 1 cut(s) 468
BspLI GGNNCC 2 cut(s) 476, 545
BspT107I GGYRCC 2 cut(s) 474, 543
BsrDI GCAATG 1 cut(s) 140
BsrFI RCCGGY 2 cut(s) 145, 270
BssAI RCCGGY 2 cut(s) 145, 270
BssECI CCNNGG 3 cut(s) 112, 482, 567
BssMI GATC 1 cut(s) 580
BssNI GRCGYC 1 cut(s) 544
BssSI CACGAG 1 cut(s) 185
BssT1I CCWWGG 1 cut(s) 482
Bst2BI CACGAG 1 cut(s) 185
Bst4CI ACNGT 1 cut(s) 11
Bst6I CTCTTC 1 cut(s) 402
BstACI GRCGYC 1 cut(s) 544
BstDEI CTNAG 1 cut(s) 527
BstEII GGTNACC 1 cut(s) 277
BstF5I GGATG 3 cut(s) 46, 75, 196
BstFNI CGCG 1 cut(s) 468
BstH2I RGCGCY 1 cut(s) 547
BstHHI GCGC 2 cut(s) 526, 546
BstKTI GATC 1 cut(s) 583
BstMBI GATC 1 cut(s) 580
BstMCI CGRYCG 1 cut(s) 239
BstPI GGTNACC 1 cut(s) 277
BstSCI CCNGG 1 cut(s) 112
BstUI CGCG 1 cut(s) 468
BstZI CGGCCG 1 cut(s) 236
BsuRI GGCC 2 cut(s) 238, 261
BtsCI GGATG 3 cut(s) 46, 75, 196
CfoI GCGC 2 cut(s) 526, 546
Cfr10I RCCGGY 2 cut(s) 145, 270
CviAII CATG 2 cut(s) 263, 455
CviJI RGCY 9 cut(s) 17, 32, 229, 238, 261, 274, 343, 402, 515
CviKI_1 RGCY 9 cut(s) 17, 32, 229, 238, 261, 274, 343, 402, 515
DdeI CTNAG 1 cut(s) 527
DinI GGCGCC 1 cut(s) 545
DpnI GATC 1 cut(s) 582
DpnII GATC 1 cut(s) 580
EaeI YGGCCR 2 cut(s) 236, 259
EagI CGGCCG 1 cut(s) 236
Eam1104I CTCTTC 1 cut(s) 402
EarI CTCTTC 1 cut(s) 402
EclXI CGGCCG 1 cut(s) 236
Eco130I CCWWGG 1 cut(s) 482
Eco52I CGGCCG 1 cut(s) 236
Eco88I CYCGRG 1 cut(s) 353
Eco91I GGTNACC 1 cut(s) 277
EcoO65I GGTNACC 1 cut(s) 277
EcoRI GAATTC 1 cut(s) 385
EcoT14I CCWWGG 1 cut(s) 482
EgeI GGCGCC 1 cut(s) 545
EheI GGCGCC 1 cut(s) 545
ErhI CCWWGG 1 cut(s) 482
FaeI CATG 2 cut(s) 266, 458
FaiI YATR 5 cut(s) 83, 264, 296, 321, 456
FatI CATG 2 cut(s) 262, 454
FbaI TGATCA 1 cut(s) 580
FokI GGATG 3 cut(s) 33, 82, 203
GlaI GCGC 2 cut(s) 525, 545
HaeII RGCGCY 1 cut(s) 547
HaeIII GGCC 2 cut(s) 238, 261
HapII CCGG 4 cut(s) 114, 146, 235, 271
HhaI GCGC 2 cut(s) 526, 546
Hin1I GRCGYC 1 cut(s) 544
Hin1II CATG 2 cut(s) 266, 458
Hin6I GCGC 2 cut(s) 524, 544
HinP1I GCGC 2 cut(s) 524, 544
HindIII AAGCTT 1 cut(s) 227
HinfI GANTC 1 cut(s) 181
HpaII CCGG 4 cut(s) 114, 146, 235, 271
HphI GGTGA 3 cut(s) 49, 109, 271
Hpy188I TCNGA 4 cut(s) 51, 154, 433, 568
Hpy188III TCNNGA 3 cut(s) 185, 287, 355
HpyAV CCTTC 4 cut(s) 292, 301, 373, 433
HpyCH4III ACNGT 1 cut(s) 11
HpyCH4IV ACGT 1 cut(s) 123
HpyCH4V TGCA 3 cut(s) 26, 556, 617
HpyF3I CTNAG 1 cut(s) 527
HpySE526I ACGT 1 cut(s) 123
Hsp92I GRCGYC 1 cut(s) 544
Hsp92II CATG 2 cut(s) 266, 458
HspAI GCGC 2 cut(s) 524, 544
KasI GGCGCC 1 cut(s) 543
Ksp22I TGATCA 1 cut(s) 580
Kzo9I GATC 1 cut(s) 580
LmnI GCTCC 1 cut(s) 370
LpnPI CCDG 7 cut(s) 20, 127, 159, 248, 260, 284, 375
LweI GCATC 3 cut(s) 13, 55, 570
MaeII ACGT 1 cut(s) 123
MaeIII GTNAC 3 cut(s) 116, 277, 574
MalI GATC 1 cut(s) 582
MboI GATC 1 cut(s) 580
MboII GAAGA 3 cut(s) 70, 343, 419
MhlI GDGCHC 2 cut(s) 198, 354
MluCI AATT 1 cut(s) 385
Mly113I GGCGCC 1 cut(s) 544
MseI TTAA 1 cut(s) 225
MslI CAYNNNNRTG 2 cut(s) 51, 189
MspI CCGG 4 cut(s) 114, 146, 235, 271
MspR9I CCNGG 1 cut(s) 114
Mva1269I GAATGC 1 cut(s) 505
MvnI CGCG 1 cut(s) 468
NarI GGCGCC 1 cut(s) 544
NciI CCSGG 1 cut(s) 114
NdeII GATC 1 cut(s) 580
NlaIII CATG 2 cut(s) 266, 458
NlaIV GGNNCC 2 cut(s) 476, 545
NmeAIII GCCGAG 1 cut(s) 237
NmuCI GTSAC 2 cut(s) 116, 277
PaeR7I CTCGAG 1 cut(s) 353
PcsI WCGNNNNNNNCGW 1 cut(s) 351
PctI GAATGC 1 cut(s) 505
PfeI GAWTC 1 cut(s) 181
PluTI GGCGCC 1 cut(s) 547
PspEI GGTNACC 1 cut(s) 277
PspN4I GGNNCC 2 cut(s) 476, 545
RseI CAYNNNNRTG 2 cut(s) 51, 189
SaqAI TTAA 1 cut(s) 225
Sau3AI GATC 1 cut(s) 580
ScrFI CCNGG 1 cut(s) 114
SduI GDGCHC 2 cut(s) 198, 354
SfaNI GCATC 3 cut(s) 13, 55, 570
SfoI GGCGCC 1 cut(s) 545
Sfr274I CTCGAG 1 cut(s) 353
SlaI CTCGAG 1 cut(s) 353
SmiMI CAYNNNNRTG 2 cut(s) 51, 189
SmlI CTYRAG 2 cut(s) 285, 353
SmoI CTYRAG 2 cut(s) 285, 353
Sse9I AATT 1 cut(s) 385
SsiI CCGC 2 cut(s) 206, 512
SspDI GGCGCC 1 cut(s) 543
StyD4I CCNGG 1 cut(s) 112
StyI CCWWGG 1 cut(s) 482
TaaI ACNGT 1 cut(s) 11
TaiI ACGT 1 cut(s) 126
TaqI TCGA 4 cut(s) 345, 354, 533, 600
TasI AATT 1 cut(s) 385
TauI GCSGC 1 cut(s) 515
TfiI GAWTC 1 cut(s) 181
Tru1I TTAA 1 cut(s) 225
Tru9I TTAA 1 cut(s) 225
TseFI GTSAC 2 cut(s) 116, 277
Tsp45I GTSAC 2 cut(s) 116, 277
TspDTI ATGAA 1 cut(s) 311
TspGWI ACGGA 1 cut(s) 383
XapI RAATTY 1 cut(s) 385
XhoI CTCGAG 1 cut(s) 353
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.