pycom16g08630

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0014722
Physical Location & Seq
Reverse (-)
5762263 .. 5763065
803 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 564 bp
ATGATGACTGCCATGGTCCAGTTCTTCAAATTGCCTCCTGAAGAAAGGGCAAAATACTTCACCACAGATCACTCCAAGCAAGTAAAGCTTTTCAATTACTACCTCAAAGTTGAAGAGCAAGAGCAAAATGTCACTATGTGGAGTGAAACCTTCTCAAGCAAGAGCAAAAGGTCACTATGTGGAGTGAAATTCACTCTTCTCACATCTTTGGCATCCTCTCAATTCCTTCTTTTTGCTGAATATGCAAAGGAGATTGGCAGATTGATGAAAATGCTCTTGGGATTGCTATCCCAGGGACTTGGACTAGAGAAGGACTGCTTACAAAAGAAGCTGGGTGATAATCCTACTCTGATAGCACAGGGAAACTACTATCCACCATGTCCAGACCCTGAGGTCACGCTTGGACTAGCTATCCATACTAATCTCAATGCACTCACAGTACTCAGGCGGACAGAGGGAGTGACTGGCCTTCAAGTGATCAAAGATGGGAAATGGGTTGCTGTCGATTCTCTCCCCAATGCCTTTGTTATCAATCTTGGTGACCAAATTCAGGTGCTGTCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

188

Amino Acids

20.98

Weight (kDa)

8.37

Isoelectric Point (pI)

39.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
2OG-FeII_Oxy PF03171 122 - 187 4.5e-18 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014722)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g25590
malus_domestica MD16G1101600.v1.1 MD16G1101900.v1.1
prunus_persica Prupe.8G011800_v2.0.a1
pyrus_communis pycom16g08630
rosa_chinensis RchiOBHm_Chr5g0016311
rosa_laevigata RLG00000006792
rosa_multiflora Rmu_co8454895.1_g000001 Rmu_sc0001762.1_g000036
rosa_roxburghii Rroxscaffold_5G00374460
rosa_samantha Rh1AG277500 Rh4AG318700 Rh4BG326800 Rh4CG342200 Rh4DG322000 Rh5CG136300
rosa_wichuraiana Rw4G022550

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 392
AciI CCGC 1 cut(s) 448
AcsI RAATTY 2 cut(s) 188, 546
AcuI CTGAAG 1 cut(s) 60
AdeI CACNNNGTG 2 cut(s) 138, 179
AfaI GTAC 1 cut(s) 441
AfiI CCNNNNNNNGG 1 cut(s) 550
AgsI TTSAA 4 cut(s) 28, 94, 113, 473
AjnI CCWGG 1 cut(s) 291
AjuI GAANNNNNNNTTGG 2 cut(s) 260, 292
AluBI AGCT 3 cut(s) 88, 331, 410
AluI AGCT 3 cut(s) 88, 331, 410
AlwNI CAGNNNCTG 2 cut(s) 389, 556
AoxI GGCC 1 cut(s) 466
ApoI RAATTY 2 cut(s) 188, 546
AspS9I GGNCC 1 cut(s) 16
AsuHPI GGTGA 3 cut(s) 52, 347, 551
AvaII GGWCC 1 cut(s) 16
AxyI CCTNAGG 1 cut(s) 390
BccI CCATC 1 cut(s) 479
BciT130I CCWGG 1 cut(s) 293
BclI TGATCA 1 cut(s) 477
BfaI CTAG 2 cut(s) 305, 407
BmcAI AGTACT 1 cut(s) 441
Bme1390I CCNGG 1 cut(s) 293
Bme18I GGWCC 1 cut(s) 16
BmgT120I GGNCC 1 cut(s) 16
BmrFI CCNGG 1 cut(s) 293
BmsI GCATC 1 cut(s) 221
BpuEI CTTGAG 1 cut(s) 139
BsaBI GATNNNNATC 1 cut(s) 286
BsaJI CCNNGG 3 cut(s) 12, 291, 292
Bsc4I CCNNNNNNNGG 1 cut(s) 550
Bse1I ACTGG 2 cut(s) 19, 469
Bse21I CCTNAGG 1 cut(s) 390
Bse8I GATNNNNATC 1 cut(s) 286
BseBI CCWGG 1 cut(s) 293
BseDI CCNNGG 3 cut(s) 12, 291, 292
BseGI GGATG 1 cut(s) 212
BseJI GATNNNNATC 1 cut(s) 286
BseLI CCNNNNNNNGG 1 cut(s) 550
BseMII CTCAG 2 cut(s) 381, 457
BseNI ACTGG 2 cut(s) 19, 469
BseYI CCCAGC 1 cut(s) 331
BshFI GGCC 1 cut(s) 468
BslFI GGGAC 1 cut(s) 309
BslI CCNNNNNNNGG 1 cut(s) 550
BsmFI GGGAC 1 cut(s) 309
BsnI GGCC 1 cut(s) 468
Bsp143I GATC 2 cut(s) 67, 477
Bsp19I CCATGG 1 cut(s) 12
BspACI CCGC 1 cut(s) 448
BspANI GGCC 1 cut(s) 468
BspCNI CTCAG 2 cut(s) 382, 456
BspQI GCTCTTC 1 cut(s) 108
BsrI ACTGG 2 cut(s) 19, 469
BssECI CCNNGG 3 cut(s) 12, 291, 292
BssMI GATC 2 cut(s) 67, 477
BssT1I CCWWGG 1 cut(s) 12
Bst2UI CCWGG 1 cut(s) 293
Bst4CI ACNGT 1 cut(s) 439
Bst6I CTCTTC 2 cut(s) 108, 201
BstDEI CTNAG 2 cut(s) 390, 443
BstDSI CCRYGG 1 cut(s) 12
BstEII GGTNACC 1 cut(s) 539
BstF5I GGATG 1 cut(s) 212
BstKTI GATC 2 cut(s) 70, 480
BstMBI GATC 2 cut(s) 67, 477
BstMWI GCNNNNNNNGC 2 cut(s) 85, 242
BstNI CCWGG 1 cut(s) 293
BstPI GGTNACC 1 cut(s) 539
BstSCI CCNGG 1 cut(s) 291
BstXI CCANNNNNNTGG 1 cut(s) 299
Bsu36I CCTNAGG 1 cut(s) 390
BsuRI GGCC 1 cut(s) 468
BtgI CCRYGG 1 cut(s) 12
BtsCI GGATG 1 cut(s) 212
CaiI CAGNNNCTG 2 cut(s) 389, 556
Cfr13I GGNCC 1 cut(s) 16
Csp6I GTAC 1 cut(s) 440
CviAII CATG 2 cut(s) 13, 378
CviJI RGCY 4 cut(s) 88, 331, 410, 468
CviKI_1 RGCY 4 cut(s) 88, 331, 410, 468
CviQI GTAC 1 cut(s) 440
DdeI CTNAG 2 cut(s) 390, 443
DpnI GATC 2 cut(s) 69, 479
DpnII GATC 2 cut(s) 67, 477
DraIII CACNNNGTG 2 cut(s) 138, 179
DrdI GACNNNNNNGTC 1 cut(s) 392
DseDI GACNNNNNNGTC 1 cut(s) 392
Eam1104I CTCTTC 2 cut(s) 108, 201
EarI CTCTTC 2 cut(s) 108, 201
EciI GGCGGA 1 cut(s) 463
Eco130I CCWWGG 1 cut(s) 12
Eco47I GGWCC 1 cut(s) 16
Eco57I CTGAAG 1 cut(s) 60
Eco81I CCTNAGG 1 cut(s) 390
Eco91I GGTNACC 1 cut(s) 539
EcoO65I GGTNACC 1 cut(s) 539
EcoRII CCWGG 1 cut(s) 291
EcoT14I CCWWGG 1 cut(s) 12
ErhI CCWWGG 1 cut(s) 12
FaeI CATG 2 cut(s) 16, 381
FaiI YATR 7 cut(s) 14, 137, 178, 243, 379, 417, 562
FalI AAGNNNNNCTT 4 cut(s) 72, 104, 302, 334
FaqI GGGAC 1 cut(s) 309
FatI CATG 2 cut(s) 12, 377
FbaI TGATCA 1 cut(s) 477
FokI GGATG 1 cut(s) 199
FspBI CTAG 2 cut(s) 305, 407
GsaI CCCAGC 1 cut(s) 335
HaeIII GGCC 1 cut(s) 468
Hin1II CATG 2 cut(s) 16, 381
HindIII AAGCTT 1 cut(s) 86
HinfI GANTC 1 cut(s) 506
HphI GGTGA 3 cut(s) 52, 347, 551
Hpy188I TCNGA 1 cut(s) 351
Hpy188III TCNNGA 2 cut(s) 38, 383
HpyAV CCTTC 4 cut(s) 160, 236, 304, 479
HpyCH4III ACNGT 1 cut(s) 439
HpyCH4V TGCA 2 cut(s) 245, 431
HpyF10VI GCNNNNNNNGC 2 cut(s) 85, 242
HpyF3I CTNAG 2 cut(s) 390, 443
Hsp92II CATG 2 cut(s) 16, 381
Ksp22I TGATCA 1 cut(s) 477
Kzo9I GATC 2 cut(s) 67, 477
LguI GCTCTTC 1 cut(s) 108
LweI GCATC 1 cut(s) 221
MaeI CTAG 2 cut(s) 305, 407
MaeIII GTNAC 5 cut(s) 130, 171, 394, 460, 539
MalI GATC 2 cut(s) 69, 479
MboI GATC 2 cut(s) 67, 477
MboII GAAGA 4 cut(s) 16, 53, 125, 188
MluCI AATT 5 cut(s) 29, 94, 188, 221, 546
MnlI CCTC 5 cut(s) 45, 113, 226, 385, 448
MspR9I CCNGG 1 cut(s) 293
MvaI CCWGG 1 cut(s) 293
MwoI GCNNNNNNNGC 2 cut(s) 85, 242
NcoI CCATGG 1 cut(s) 12
NdeII GATC 2 cut(s) 67, 477
NlaIII CATG 2 cut(s) 16, 381
NmuCI GTSAC 5 cut(s) 130, 171, 394, 460, 539
PasI CCCWGGG 1 cut(s) 292
PciSI GCTCTTC 1 cut(s) 108
PfeI GAWTC 1 cut(s) 506
Psp6I CCWGG 1 cut(s) 291
PspEI GGTNACC 1 cut(s) 539
PspFI CCCAGC 1 cut(s) 331
PspGI CCWGG 1 cut(s) 291
PspPI GGNCC 1 cut(s) 16
PstNI CAGNNNCTG 2 cut(s) 389, 556
RsaI GTAC 1 cut(s) 441
RsaNI GTAC 1 cut(s) 440
SapI GCTCTTC 1 cut(s) 108
Sau3AI GATC 2 cut(s) 67, 477
Sau96I GGNCC 1 cut(s) 16
ScaI AGTACT 1 cut(s) 441
ScrFI CCNGG 1 cut(s) 293
SetI ASST 8 cut(s) 90, 105, 152, 173, 333, 396, 412, 555
SfaNI GCATC 1 cut(s) 221
SinI GGWCC 1 cut(s) 16
SmlI CTYRAG 1 cut(s) 154
SmoI CTYRAG 1 cut(s) 154
Sse9I AATT 5 cut(s) 29, 94, 188, 221, 546
SsiI CCGC 1 cut(s) 448
SspMI CTAG 2 cut(s) 305, 407
StyD4I CCNGG 1 cut(s) 291
StyI CCWWGG 1 cut(s) 12
TaaI ACNGT 1 cut(s) 439
TaqI TCGA 1 cut(s) 504
TasI AATT 5 cut(s) 29, 94, 188, 221, 546
TatI WGTACW 1 cut(s) 439
TfiI GAWTC 1 cut(s) 506
TseFI GTSAC 5 cut(s) 130, 171, 394, 460, 539
Tsp45I GTSAC 5 cut(s) 130, 171, 394, 460, 539
TspDTI ATGAA 1 cut(s) 281
VpaK11BI GGWCC 1 cut(s) 16
XapI RAATTY 2 cut(s) 188, 546
XspI CTAG 2 cut(s) 305, 407
ZrmI AGTACT 1 cut(s) 441
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.