pycom16g11190

Random slug protein 5-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0011976
Physical Location & Seq
Reverse (-)
7770773 .. 7773349
2577 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 774 bp
ATGGAATCATCGAATGGGAATGGGAGTGGGAAAGGGATGCAAGAAGCATATGAGGAAGTGGAAGAAGAAGCAAACTTTGAGAGCGACGAGATAGAGAGAAACAAAATGGGAATCATGAGAGCACTTGTGGAAAGAGAAGATCCCTCCTCCAAGGATGTGGATGATTCCATGATTCGGAGATTTCTTCGGGCGCGTGATCTGGATATTGAGAAGGCTACTAACATGTTCCTCAAGTATCTGAGTTGGAAGAAGCCATTTGTGCCAAATGGTTCAATCTCGGAGTCAGAGATTCCAAAACAACTTGCTCAGAACAAGCTGTTTATGCAAGGTGTGGACAAGACGGGACGCCCCATTGTGGTTGTGTATGGTGGGCGGCATAAGCAGACCACACTCGAAGAGTTCAAACGTTTTGTAGTCTACACCCTTGAAAAAATATGTTCCAGAATGCCAGCAGGAAAGGAAAAGTTTGTGTCCATTGCAGATATTGAAGGATGGGGATATACCAATAGCGATGTTCGTGGATACTTGGCAGCTCTGACAATCTTACAGGACTGCTACCCGGAGAGGCTTGGCAAGTTATTTCTTGTCCATGTGCCTTATGTATTCATGACTGCTTGGAAGATGGTTTACCCATTTATTGACAGCAAAACCAAAAAGAAGATCATTTTTGTCGAAAACAAAAAGCTGAACTCAACCTTGCTGAAAGATATCGATGAGAGCCAGCTCCCAGAGGTTCCGGAAACATTTCATCTGGCGTTGCAAGAGATGGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CRAL_TRIO

Protein Analysis

258

Amino Acids

29.62

Weight (kDa)

5.66

Isoelectric Point (pI)

47.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CRAL_TRIO PF00650 103 - 244 5.2e-36 CRAL/TRIO domain
CRAL_TRIO_2 PF13716 115 - 244 2.9e-09 Divergent CRAL/TRIO domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011976)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G01630
fragaria_vesca FvH4_4g22590
malus_domestica MD13G1131200.v1.1 MD16G1141400.v1.1
prunus_persica Prupe.1G214400_v2.0.a1
pyrus_communis pycom13g11450 pycom16g11190
rosa_chinensis RchiOBHm_Chr4g0428831
rosa_laevigata RLG00000007122
rosa_multiflora Rmu_sc0008612.1_g000001
rosa_roxburghii Rroxscaffold_5G00370510
rosa_rugosa Rorug04G0229300 Rorug04G0229400 Rorug04G0229500
rosa_samantha Rh4AG286200 Rh4BG292000 Rh4CG308000 Rh4DG289200
rosa_wichuraiana Rw4G024810

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 417
AccII CGCG 1 cut(s) 193
AccIII TCCGGA 1 cut(s) 736
AciI CCGC 1 cut(s) 373
AclI AACGTT 1 cut(s) 406
AclWI GGATC 1 cut(s) 134
AcyI GRCGYC 1 cut(s) 346
AfiI CCNNNNNNNGG 2 cut(s) 174, 355
AflIII ACRYGT 1 cut(s) 222
AgsI TTSAA 4 cut(s) 273, 403, 428, 488
AluBI AGCT 4 cut(s) 316, 533, 685, 724
AluI AGCT 4 cut(s) 316, 533, 685, 724
Alw21I GWGCWC 1 cut(s) 124
AlwI GGATC 1 cut(s) 134
Aor13HI TCCGGA 1 cut(s) 736
ApeKI GCWGC 1 cut(s) 530
Asp700I GAANNNNTTC 1 cut(s) 744
AspLEI GCGC 1 cut(s) 193
AsuC2I CCSGG 1 cut(s) 560
BarI GAAGNNNNNNTAC 2 cut(s) 611, 643
Bbv12I GWGCWC 1 cut(s) 124
BbvI GCAGC 1 cut(s) 542
BccI CCATC 3 cut(s) 486, 616, 760
BciVI GTATCC 1 cut(s) 515
BcnI CCSGG 1 cut(s) 560
BfuI GTATCC 1 cut(s) 515
BisI GCNGC 2 cut(s) 374, 531
BlsI GCNGC 2 cut(s) 375, 532
Bme1390I CCNGG 1 cut(s) 560
BmiI GGNNCC 1 cut(s) 735
BmrFI CCNGG 1 cut(s) 560
BmsI GCATC 1 cut(s) 27
BpuEI CTTGAG 1 cut(s) 215
BpuMI CCSGG 1 cut(s) 560
Bsa29I ATCGAT 1 cut(s) 711
BsaHI GRCGYC 1 cut(s) 346
BsaJI CCNNGG 1 cut(s) 150
BsaWI WCCGGW 1 cut(s) 736
Bsc4I CCNNNNNNNGG 2 cut(s) 174, 355
Bse3DI GCAATG 1 cut(s) 474
BseAI TCCGGA 1 cut(s) 736
BseCI ATCGAT 1 cut(s) 711
BseDI CCNNGG 1 cut(s) 150
BseGI GGATG 4 cut(s) 42, 160, 166, 497
BseLI CCNNNNNNNGG 2 cut(s) 174, 355
BseMI GCAATG 1 cut(s) 474
BseMII CTCAG 2 cut(s) 230, 320
BseRI GAGGAG 1 cut(s) 136
BseXI GCAGC 1 cut(s) 542
Bsh1236I CGCG 1 cut(s) 193
BshVI ATCGAT 1 cut(s) 711
BsiHKAI GWGCWC 1 cut(s) 124
BsiSI CCGG 2 cut(s) 560, 737
BslFI GGGAC 1 cut(s) 357
BslI CCNNNNNNNGG 2 cut(s) 174, 355
BsmFI GGGAC 1 cut(s) 357
BsmI GAATGC 1 cut(s) 450
Bsp1286I GDGCHC 1 cut(s) 124
Bsp13I TCCGGA 1 cut(s) 736
Bsp143I GATC 3 cut(s) 139, 196, 660
BspACI CCGC 1 cut(s) 373
BspCNI CTCAG 2 cut(s) 231, 319
BspDI ATCGAT 1 cut(s) 711
BspEI TCCGGA 1 cut(s) 736
BspFNI CGCG 1 cut(s) 193
BspHI TCATGA 2 cut(s) 114, 606
BspLI GGNNCC 1 cut(s) 735
BspPI GGATC 1 cut(s) 134
BsrDI GCAATG 1 cut(s) 474
BssECI CCNNGG 1 cut(s) 150
BssMI GATC 3 cut(s) 139, 196, 660
BssNI GRCGYC 1 cut(s) 346
BssT1I CCWWGG 1 cut(s) 150
Bst6I CTCTTC 1 cut(s) 390
BstACI GRCGYC 1 cut(s) 346
BstC8I GCNNGC 2 cut(s) 450, 722
BstDEI CTNAG 2 cut(s) 239, 306
BstF5I GGATG 4 cut(s) 42, 160, 166, 497
BstFNI CGCG 1 cut(s) 193
BstHHI GCGC 1 cut(s) 193
BstKTI GATC 3 cut(s) 142, 199, 663
BstMBI GATC 3 cut(s) 139, 196, 660
BstMWI GCNNNNNNNGC 3 cut(s) 259, 322, 379
BstNSI RCATGY 1 cut(s) 226
BstSCI CCNGG 1 cut(s) 558
BstUI CGCG 1 cut(s) 193
BstV1I GCAGC 1 cut(s) 542
BstX2I RGATCY 1 cut(s) 139
BstXI CCANNNNNNTGG 1 cut(s) 157
BstYI RGATCY 1 cut(s) 139
Bsu15I ATCGAT 1 cut(s) 711
BsuI GTATCC 1 cut(s) 515
BsuTUI ATCGAT 1 cut(s) 711
BtgZI GCGATG 1 cut(s) 525
BtsCI GGATG 4 cut(s) 42, 160, 166, 497
Cac8I GCNNGC 2 cut(s) 450, 722
CciI TCATGA 2 cut(s) 114, 606
CfoI GCGC 1 cut(s) 193
ClaI ATCGAT 1 cut(s) 711
CseI GACGC 1 cut(s) 354
CviAII CATG 5 cut(s) 115, 169, 223, 590, 607
CviJI RGCY 8 cut(s) 215, 253, 316, 533, 568, 685, 720, 724
CviKI_1 RGCY 8 cut(s) 215, 253, 316, 533, 568, 685, 720, 724
DdeI CTNAG 2 cut(s) 239, 306
DpnI GATC 3 cut(s) 141, 198, 662
DpnII GATC 3 cut(s) 139, 196, 660
Eam1104I CTCTTC 1 cut(s) 390
EarI CTCTTC 1 cut(s) 390
Eco130I CCWWGG 1 cut(s) 150
Eco32I GATATC 1 cut(s) 709
EcoRV GATATC 1 cut(s) 709
EcoT14I CCWWGG 1 cut(s) 150
ErhI CCWWGG 1 cut(s) 150
FaeI CATG 5 cut(s) 118, 172, 226, 593, 610
FaqI GGGAC 1 cut(s) 357
FatI CATG 5 cut(s) 114, 168, 222, 589, 606
FauNDI CATATG 1 cut(s) 49
FblI GTMKAC 1 cut(s) 417
Fnu4HI GCNGC 2 cut(s) 374, 531
FokI GGATG 4 cut(s) 49, 167, 173, 504
Fsp4HI GCNGC 2 cut(s) 374, 531
GlaI GCGC 1 cut(s) 192
GluI GCNGC 2 cut(s) 374, 531
HapII CCGG 2 cut(s) 560, 737
HgaI GACGC 1 cut(s) 354
HhaI GCGC 1 cut(s) 193
Hin1I GRCGYC 1 cut(s) 346
Hin1II CATG 5 cut(s) 118, 172, 226, 593, 610
Hin6I GCGC 1 cut(s) 191
HinP1I GCGC 1 cut(s) 191
HinfI GANTC 6 cut(s) 5, 111, 164, 172, 281, 289
HpaII CCGG 2 cut(s) 560, 737
Hpy166II GTNNAC 3 cut(s) 334, 418, 628
Hpy188I TCNGA 6 cut(s) 177, 240, 280, 286, 309, 537
Hpy188III TCNNGA 5 cut(s) 115, 200, 441, 607, 737
Hpy8I GTNNAC 3 cut(s) 334, 418, 628
Hpy99I CGWCG 1 cut(s) 89
HpyAV CCTTC 2 cut(s) 205, 482
HpyCH4IV ACGT 1 cut(s) 406
HpyCH4V TGCA 4 cut(s) 40, 325, 479, 760
HpyF10VI GCNNNNNNNGC 3 cut(s) 259, 322, 379
HpyF3I CTNAG 2 cut(s) 239, 306
HpySE526I ACGT 1 cut(s) 406
Hsp92I GRCGYC 1 cut(s) 346
Hsp92II CATG 5 cut(s) 118, 172, 226, 593, 610
HspAI GCGC 1 cut(s) 191
Kpn2I TCCGGA 1 cut(s) 736
Kzo9I GATC 3 cut(s) 139, 196, 660
LmnI GCTCC 1 cut(s) 729
Lsp1109I GCAGC 1 cut(s) 542
LweI GCATC 1 cut(s) 27
MaeII ACGT 1 cut(s) 406
MalI GATC 3 cut(s) 141, 198, 662
MboI GATC 3 cut(s) 139, 196, 660
MboII GAAGA 8 cut(s) 74, 77, 149, 176, 259, 407, 631, 670
MflI RGATCY 1 cut(s) 139
MhlI GDGCHC 1 cut(s) 124
MlyI GAGTC 1 cut(s) 290
MmeI TCCRAC 1 cut(s) 224
MnlI CCTC 6 cut(s) 46, 154, 157, 239, 558, 724
MroI TCCGGA 1 cut(s) 736
MroXI GAANNNNTTC 1 cut(s) 744
MspI CCGG 2 cut(s) 560, 737
MspR9I CCNGG 1 cut(s) 560
Mva1269I GAATGC 1 cut(s) 450
MvnI CGCG 1 cut(s) 193
MwoI GCNNNNNNNGC 3 cut(s) 259, 322, 379
NciI CCSGG 1 cut(s) 560
NdeI CATATG 1 cut(s) 49
NdeII GATC 3 cut(s) 139, 196, 660
NlaIII CATG 5 cut(s) 118, 172, 226, 593, 610
NlaIV GGNNCC 1 cut(s) 735
NspI RCATGY 1 cut(s) 226
PagI TCATGA 2 cut(s) 114, 606
PciI ACATGT 1 cut(s) 222
PctI GAATGC 1 cut(s) 450
PdmI GAANNNNTTC 1 cut(s) 744
PfeI GAWTC 5 cut(s) 5, 111, 164, 172, 289
PkrI GCNGC 2 cut(s) 375, 532
PleI GAGTC 1 cut(s) 289
PpsI GAGTC 1 cut(s) 289
PscI ACATGT 1 cut(s) 222
Psp1406I AACGTT 1 cut(s) 406
PspN4I GGNNCC 1 cut(s) 735
PsuI RGATCY 1 cut(s) 139
SatI GCNGC 2 cut(s) 374, 531
Sau3AI GATC 3 cut(s) 139, 196, 660
SchI GAGTC 1 cut(s) 290
ScrFI CCNGG 1 cut(s) 560
SduI GDGCHC 1 cut(s) 124
SetI ASST 8 cut(s) 318, 331, 409, 535, 687, 698, 726, 735
SfaNI GCATC 1 cut(s) 27
SmlI CTYRAG 1 cut(s) 230
SmoI CTYRAG 1 cut(s) 230
SsiI CCGC 1 cut(s) 373
StyD4I CCNGG 1 cut(s) 558
StyI CCWWGG 1 cut(s) 150
TaiI ACGT 1 cut(s) 409
TaqI TCGA 4 cut(s) 11, 393, 672, 711
TauI GCSGC 1 cut(s) 376
TfiI GAWTC 5 cut(s) 5, 111, 164, 172, 289
TseI GCWGC 1 cut(s) 530
TspDTI ATGAA 2 cut(s) 595, 737
XceI RCATGY 1 cut(s) 226
XmiI GTMKAC 1 cut(s) 417
XmnI GAANNNNTTC 1 cut(s) 744
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.