pycom16g12420

YT521-B-like domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0006149
Physical Location & Seq
Forward (+)
8716384 .. 8718567
2184 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1467 bp
ATGGATCGATCAAGCAAGCATGCTGCGACACATGGAGGCGTTTCTTCTGGTCGTTTTCCAACCCCAGCCTCATCACATGTTAATCAGGTTAGAAGTGCATCCAGGTCAATTTCGTCTGTGGATAAACTTCCAAATGGAAAAGTATTGCCTAATAATTTCAAGGTGGAAATCCCCATTGGTAATGGCTTATCTAACTTTGGATCTAGTGCTCAAGGAAGTGTTGCTGTGAGTAAGCTTCGACCAAAGTTTCATGTTGGCAGGGACTTGAATGATGTACATGGTGGCCTAGAGGCATTGGGTGAGCAAAATTGTGGTCCTAGAATCAATAGGTTAAAAAATCAACTAGCTGTGAAAGCCTATACGACCAAGGCAGGAGATTGTGATGCACAAGGAAACATCATTATCCGTACTGATAAATATAATAAGGATGACCTGCCAGTTGACTACGCGGATGCAAAGTTTTTCGTAATAAAATCTTACAGCGAGGATGATGTTCACAAAAGTGTCAAATACAATGTCTGGTCATCCACACCCCATGGCAACAAGAAACTAAATAGTGCATATGAAGATGCTCAAAGAATAGCTGCAGGAAAACATAGAGCCTGTCCTATCTTCCTTTTCTTTTCGGTTAATGCAAGTGGTCAATTCTGTGGCATAGCGGAGATGGGTGGCCCGGTTGACTTTAACAAGGATATGGACTTTTGGCAACAAGATAAATGGAGTGGGAGCTTCCCTGTTAAGTGGCACATCATTAAAGATGTTCCAAATAACAGTTTCAGGCACATTGTATTGGAGAACAATGAAAACAAGCCGGTGACTAATAGCAGGGATACACAAGAGGTCATGTATAAGAAAGGCTTGGAGATTCTGAAAATATTCAAGAACCACACATTGAAGACATCTTTACTTGATGACTTTATGTACTACGAAGACCGTCAGAAAATCATGCAGGAAGAGAGAGGCAGGTTTCTTATCAGAAGCTTCGAAAGTCCATTTTTAGTAACTGGGCTGGGCCCCCCTCGCAAGCTGAACAACTCTGTGGGTGAGCTGCCCTGTATTAAGGAGGAAAAAGACACCAAACTTAACGACGATGCAAACAACTCAAAAAAGATTACAGTCTCTGTTGCTGAGCTGGTTTCCTCCAATATGAGTGCTAAAACAAGTATCAAGAAGGAAAACACTGAGCAGAAGGCAATTGAAGCAAAAGATGATGTTGTGTCTACCTTAGAAGTTGGTTCCCTCACTATTAATCCAAAGCAGGCAGGATCAAAATCTTCAGCAGCTGCTGTTACTAACACCGGAAAGGTTGATGTTGTCACTGTGGGCTCAATGCCCGTAAGAGTTAATGGATATGCTGAATCCTTGGGTAATTTAACGTTTGGAACAATCCCTCTGGATCCTAGAGCGTTACGACTAGAGAAGGGCAGCTCTATTATCGAACAGGCTGCTCAACCTCAACAGAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
YTH

Protein Analysis

489

Amino Acids

53.66

Weight (kDa)

9.16

Isoelectric Point (pI)

33.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
YTH PF04146 152 - 294 1.5e-49 YT521-B-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 441, 954
AccI GTMKAC 1 cut(s) 1220
AccII CGCG 1 cut(s) 449
AciI CCGC 2 cut(s) 449, 659
AclI AACGTT 1 cut(s) 1376
AclWI GGATC 5 cut(s) 12, 208, 1273, 1391, 1404
AcuI CTGAAG 1 cut(s) 1260
AfaI GTAC 3 cut(s) 276, 409, 923
AflIII ACRYGT 1 cut(s) 76
AgsI TTSAA 5 cut(s) 160, 268, 880, 895, 1199
AjnI CCWGG 1 cut(s) 101
AjuI GAANNNNNNNTTGG 2 cut(s) 159, 191
AleI CACNNNNGTG 1 cut(s) 501
Alw21I GWGCWC 1 cut(s) 211
Alw26I GTCTC 1 cut(s) 1123
AlwI GGATC 5 cut(s) 12, 208, 1273, 1391, 1404
AlwNI CAGNNNCTG 3 cut(s) 1121, 1283, 1286
AoxI GGCC 3 cut(s) 283, 670, 1012
ApaI GGGCCC 1 cut(s) 1016
ApeKI GCWGC 7 cut(s) 23, 584, 1048, 1280, 1283, 1425, 1445
ArsI GACNNNNNNTTYG 4 cut(s) 298, 330, 501, 533
AseI ATTAAT 1 cut(s) 1248
Asp700I GAANNNNTTC 1 cut(s) 875
AspS9I GGNCC 4 cut(s) 314, 671, 1012, 1013
AsuC2I CCSGG 1 cut(s) 674
AsuHPI GGTGA 3 cut(s) 311, 826, 1055
AsuII TTCGAA 1 cut(s) 984
AvaII GGWCC 1 cut(s) 314
BaeGI GKGCMC 1 cut(s) 1016
BamHI GGATCC 1 cut(s) 1396
BanII GRGCYC 2 cut(s) 1016, 1328
BbsI GAAGAC 2 cut(s) 902, 936
Bbv12I GWGCWC 1 cut(s) 211
BbvI GCAGC 7 cut(s) 10, 571, 1035, 1270, 1292, 1432, 1437
BccI CCATC 1 cut(s) 658
BcgI CGANNNNNNTGC 2 cut(s) 1427, 1461
BciT130I CCWGG 1 cut(s) 103
BciVI GTATCC 1 cut(s) 823
BcnI CCSGG 1 cut(s) 674
BcoDI GTCTC 1 cut(s) 1123
BfaI CTAG 6 cut(s) 204, 287, 318, 344, 1401, 1415
BfmI CTRYAG 1 cut(s) 585
BfuAI ACCTGC 2 cut(s) 441, 954
BfuI GTATCC 1 cut(s) 823
BisI GCNGC 7 cut(s) 24, 585, 1049, 1281, 1284, 1426, 1446
BlpI GCTNAGC 1 cut(s) 1128
BlsI GCNGC 7 cut(s) 25, 586, 1050, 1282, 1285, 1427, 1447
Bme1390I CCNGG 2 cut(s) 103, 674
Bme18I GGWCC 1 cut(s) 314
BmgT120I GGNCC 4 cut(s) 314, 671, 1012, 1013
BmiI GGNNCC 4 cut(s) 1014, 1015, 1237, 1398
BmrFI CCNGG 2 cut(s) 103, 674
BmrI ACTGGG 1 cut(s) 1014
BmsI GCATC 5 cut(s) 107, 373, 442, 559, 1081
BmuI ACTGGG 1 cut(s) 1014
BpiI GAAGAC 2 cut(s) 902, 936
Bpu1102I GCTNAGC 1 cut(s) 1128
Bpu14I TTCGAA 1 cut(s) 984
BpuEI CTTGAG 1 cut(s) 195
BpuMI CCSGG 1 cut(s) 674
Bsa29I ATCGAT 1 cut(s) 7
BsaBI GATNNNNATC 1 cut(s) 1270
BsaJI CCNNGG 3 cut(s) 366, 535, 1362
BsaWI WCCGGW 1 cut(s) 1298
Bse118I RCCGGY 1 cut(s) 811
Bse1I ACTGG 2 cut(s) 437, 1009
Bse8I GATNNNNATC 1 cut(s) 1270
BseBI CCWGG 1 cut(s) 103
BseCI ATCGAT 1 cut(s) 7
BseDI CCNNGG 3 cut(s) 366, 535, 1362
BseGI GGATG 5 cut(s) 98, 433, 457, 493, 524
BseJI GATNNNNATC 1 cut(s) 1270
BseMII CTCAG 2 cut(s) 1119, 1173
BseNI ACTGG 2 cut(s) 437, 1009
BseSI GKGCMC 1 cut(s) 1016
BseXI GCAGC 7 cut(s) 10, 571, 1035, 1270, 1292, 1432, 1437
BseYI CCCAGC 2 cut(s) 64, 1009
Bsh1236I CGCG 1 cut(s) 449
BshFI GGCC 3 cut(s) 285, 672, 1014
BshVI ATCGAT 1 cut(s) 7
BsiHKAI GWGCWC 1 cut(s) 211
BsiSI CCGG 3 cut(s) 674, 812, 1299
BslFI GGGAC 1 cut(s) 275
BsmAI GTCTC 1 cut(s) 1123
BsmFI GGGAC 1 cut(s) 275
BsnI GGCC 3 cut(s) 285, 672, 1014
Bsp119I TTCGAA 1 cut(s) 984
Bsp120I GGGCCC 1 cut(s) 1012
Bsp1286I GDGCHC 3 cut(s) 211, 1016, 1328
Bsp1407I TGTACA 1 cut(s) 274
Bsp143I GATC 5 cut(s) 4, 8, 200, 1265, 1396
Bsp1720I GCTNAGC 1 cut(s) 1128
Bsp19I CCATGG 1 cut(s) 535
BspACI CCGC 2 cut(s) 449, 659
BspANI GGCC 3 cut(s) 285, 672, 1014
BspCNI CTCAG 2 cut(s) 1120, 1174
BspDI ATCGAT 1 cut(s) 7
BspFNI CGCG 1 cut(s) 449
BspLI GGNNCC 4 cut(s) 1014, 1015, 1237, 1398
BspMAI CTGCAG 1 cut(s) 589
BspMI ACCTGC 2 cut(s) 441, 954
BspPI GGATC 5 cut(s) 12, 208, 1273, 1391, 1404
BspT104I TTCGAA 1 cut(s) 984
BsrFI RCCGGY 1 cut(s) 811
BsrGI TGTACA 1 cut(s) 274
BsrI ACTGG 2 cut(s) 437, 1009
BssAI RCCGGY 1 cut(s) 811
BssECI CCNNGG 3 cut(s) 366, 535, 1362
BssMI GATC 5 cut(s) 4, 8, 200, 1265, 1396
BssT1I CCWWGG 3 cut(s) 366, 535, 1362
Bst2UI CCWGG 1 cut(s) 103
Bst4CI ACNGT 4 cut(s) 773, 935, 1117, 1321
Bst6I CTCTTC 1 cut(s) 948
BstAUI TGTACA 1 cut(s) 274
BstBI TTCGAA 1 cut(s) 984
BstC8I GCNNGC 4 cut(s) 17, 21, 1025, 1260
BstDEI CTNAG 3 cut(s) 1128, 1182, 1225
BstDSI CCRYGG 1 cut(s) 535
BstF5I GGATG 5 cut(s) 98, 433, 457, 493, 524
BstFNI CGCG 1 cut(s) 449
BstKTI GATC 5 cut(s) 7, 11, 203, 1268, 1399
BstMAI GTCTC 1 cut(s) 1123
BstMBI GATC 5 cut(s) 4, 8, 200, 1265, 1396
BstMWI GCNNNNNNNGC 3 cut(s) 353, 1020, 1199
BstNI CCWGG 1 cut(s) 103
BstNSI RCATGY 2 cut(s) 23, 80
BstSCI CCNGG 2 cut(s) 101, 672
BstSFI CTRYAG 1 cut(s) 585
BstSLI GKGCMC 1 cut(s) 1016
BstUI CGCG 1 cut(s) 449
BstV1I GCAGC 7 cut(s) 10, 571, 1035, 1270, 1292, 1432, 1437
BstV2I GAAGAC 2 cut(s) 902, 936
BstX2I RGATCY 2 cut(s) 200, 1396
BstYI RGATCY 2 cut(s) 200, 1396
Bsu15I ATCGAT 1 cut(s) 7
BsuI GTATCC 1 cut(s) 823
BsuRI GGCC 3 cut(s) 285, 672, 1014
BsuTUI ATCGAT 1 cut(s) 7
BtgI CCRYGG 1 cut(s) 535
BtsCI GGATG 5 cut(s) 98, 433, 457, 493, 524
BtsIMutI CAGTG 2 cut(s) 1179, 1317
BveI ACCTGC 2 cut(s) 441, 954
Cac8I GCNNGC 4 cut(s) 17, 21, 1025, 1260
CaiI CAGNNNCTG 3 cut(s) 1121, 1283, 1286
Cfr10I RCCGGY 1 cut(s) 811
Cfr13I GGNCC 4 cut(s) 314, 671, 1012, 1013
ClaI ATCGAT 1 cut(s) 7
Csp6I GTAC 3 cut(s) 275, 408, 922
CviAII CATG 8 cut(s) 20, 32, 77, 251, 278, 536, 844, 946
CviQI GTAC 3 cut(s) 275, 408, 922
DdeI CTNAG 3 cut(s) 1128, 1182, 1225
DpnI GATC 5 cut(s) 6, 10, 202, 1267, 1398
DpnII GATC 5 cut(s) 4, 8, 200, 1265, 1396
Eam1104I CTCTTC 1 cut(s) 948
EarI CTCTTC 1 cut(s) 948
Eco130I CCWWGG 3 cut(s) 366, 535, 1362
Eco24I GRGCYC 2 cut(s) 1016, 1328
Eco47I GGWCC 1 cut(s) 314
Eco57I CTGAAG 1 cut(s) 1260
EcoO109I RGGNCCY 1 cut(s) 1013
EcoRII CCWGG 1 cut(s) 101
EcoT14I CCWWGG 3 cut(s) 366, 535, 1362
EcoT38I GRGCYC 2 cut(s) 1016, 1328
ErhI CCWWGG 3 cut(s) 366, 535, 1362
FaeI CATG 8 cut(s) 23, 35, 80, 254, 281, 539, 847, 949
FalI AAGNNNNNCTT 2 cut(s) 842, 874
FaqI GGGAC 1 cut(s) 275
FatI CATG 8 cut(s) 19, 31, 76, 250, 277, 535, 843, 945
FauNDI CATATG 1 cut(s) 562
FblI GTMKAC 1 cut(s) 1220
Fnu4HI GCNGC 7 cut(s) 24, 585, 1049, 1281, 1284, 1426, 1446
FokI GGATG 5 cut(s) 85, 440, 464, 500, 511
FriOI GRGCYC 2 cut(s) 1016, 1328
Fsp4HI GCNGC 7 cut(s) 24, 585, 1049, 1281, 1284, 1426, 1446
FspBI CTAG 6 cut(s) 204, 287, 318, 344, 1401, 1415
GluI GCNGC 7 cut(s) 24, 585, 1049, 1281, 1284, 1426, 1446
GsaI CCCAGC 2 cut(s) 68, 1013
HaeIII GGCC 3 cut(s) 285, 672, 1014
HapII CCGG 3 cut(s) 674, 812, 1299
Hin1II CATG 8 cut(s) 23, 35, 80, 254, 281, 539, 847, 949
HincII GTYRAC 2 cut(s) 442, 679
HindII GTYRAC 2 cut(s) 442, 679
HindIII AAGCTT 2 cut(s) 233, 979
HinfI GANTC 3 cut(s) 321, 865, 1358
HpaII CCGG 3 cut(s) 674, 812, 1299
HphI GGTGA 3 cut(s) 311, 826, 1055
Hpy166II GTNNAC 4 cut(s) 442, 496, 679, 1221
Hpy188I TCNGA 3 cut(s) 870, 939, 977
Hpy188III TCNNGA 3 cut(s) 880, 1168, 1394
Hpy8I GTNNAC 4 cut(s) 442, 496, 679, 1221
Hpy99I CGWCG 1 cut(s) 1091
HpyAV CCTTC 3 cut(s) 1165, 1183, 1414
HpyCH4III ACNGT 4 cut(s) 773, 935, 1117, 1321
HpyCH4IV ACGT 1 cut(s) 1376
HpyCH4V TGCA 8 cut(s) 98, 386, 455, 560, 587, 635, 949, 1094
HpyF10VI GCNNNNNNNGC 3 cut(s) 353, 1020, 1199
HpyF3I CTNAG 3 cut(s) 1128, 1182, 1225
HpySE526I ACGT 1 cut(s) 1376
Hsp92II CATG 8 cut(s) 23, 35, 80, 254, 281, 539, 847, 949
Kzo9I GATC 5 cut(s) 4, 8, 200, 1265, 1396
LmnI GCTCC 1 cut(s) 726
Lsp1109I GCAGC 7 cut(s) 10, 571, 1035, 1270, 1292, 1432, 1437
LweI GCATC 5 cut(s) 107, 373, 442, 559, 1081
MaeI CTAG 6 cut(s) 204, 287, 318, 344, 1401, 1415
MaeII ACGT 1 cut(s) 1376
MaeIII GTNAC 5 cut(s) 814, 1000, 1288, 1315, 1407
MalI GATC 5 cut(s) 6, 10, 202, 1267, 1398
MboI GATC 5 cut(s) 4, 8, 200, 1265, 1396
MboII GAAGA 7 cut(s) 36, 578, 604, 907, 941, 965, 1266
MfeI CAATTG 1 cut(s) 1194
MflI RGATCY 2 cut(s) 200, 1396
MhlI GDGCHC 3 cut(s) 211, 1016, 1328
MluCI AATT 6 cut(s) 108, 154, 307, 644, 1194, 1369
MmeI TCCRAC 1 cut(s) 83
MroXI GAANNNNTTC 1 cut(s) 875
MslI CAYNNNNRTG 1 cut(s) 501
MspA1I CMGCKG 1 cut(s) 1283
MspI CCGG 3 cut(s) 674, 812, 1299
MspR9I CCNGG 2 cut(s) 103, 674
MunI CAATTG 1 cut(s) 1194
MvaI CCWGG 1 cut(s) 103
MvnI CGCG 1 cut(s) 449
MwoI GCNNNNNNNGC 3 cut(s) 353, 1020, 1199
NciI CCSGG 1 cut(s) 674
NcoI CCATGG 1 cut(s) 535
NdeI CATATG 1 cut(s) 562
NdeII GATC 5 cut(s) 4, 8, 200, 1265, 1396
NlaIII CATG 8 cut(s) 23, 35, 80, 254, 281, 539, 847, 949
NlaIV GGNNCC 4 cut(s) 1014, 1015, 1237, 1398
NmuCI GTSAC 2 cut(s) 814, 1315
NspI RCATGY 2 cut(s) 23, 80
NspV TTCGAA 1 cut(s) 984
OliI CACNNNNGTG 1 cut(s) 501
PaeI GCATGC 1 cut(s) 23
PciI ACATGT 1 cut(s) 76
PdmI GAANNNNTTC 1 cut(s) 875
PfeI GAWTC 3 cut(s) 321, 865, 1358
PkrI GCNGC 7 cut(s) 25, 586, 1050, 1282, 1285, 1427, 1447
PscI ACATGT 1 cut(s) 76
PshBI ATTAAT 1 cut(s) 1248
Psp1406I AACGTT 1 cut(s) 1376
Psp6I CCWGG 1 cut(s) 101
PspFI CCCAGC 2 cut(s) 64, 1009
PspGI CCWGG 1 cut(s) 101
PspN4I GGNNCC 4 cut(s) 1014, 1015, 1237, 1398
PspOMI GGGCCC 1 cut(s) 1012
PspPI GGNCC 4 cut(s) 314, 671, 1012, 1013
PstI CTGCAG 1 cut(s) 589
PstNI CAGNNNCTG 3 cut(s) 1121, 1283, 1286
PsuI RGATCY 2 cut(s) 200, 1396
PvuII CAGCTG 1 cut(s) 1283
RsaI GTAC 3 cut(s) 276, 409, 923
RsaNI GTAC 3 cut(s) 275, 408, 922
RseI CAYNNNNRTG 1 cut(s) 501
SatI GCNGC 7 cut(s) 24, 585, 1049, 1281, 1284, 1426, 1446
Sau3AI GATC 5 cut(s) 4, 8, 200, 1265, 1396
Sau96I GGNCC 4 cut(s) 314, 671, 1012, 1013
ScrFI CCNGG 2 cut(s) 103, 674
SduI GDGCHC 3 cut(s) 211, 1016, 1328
SfaNI GCATC 5 cut(s) 107, 373, 442, 559, 1081
SfcI CTRYAG 1 cut(s) 585
SfuI TTCGAA 1 cut(s) 984
SinI GGWCC 1 cut(s) 314
SmiMI CAYNNNNRTG 1 cut(s) 501
SmlI CTYRAG 1 cut(s) 210
SmoI CTYRAG 1 cut(s) 210
SphI GCATGC 1 cut(s) 23
Sse9I AATT 6 cut(s) 108, 154, 307, 644, 1194, 1369
SsiI CCGC 2 cut(s) 449, 659
SspI AATATT 1 cut(s) 876
SspMI CTAG 6 cut(s) 204, 287, 318, 344, 1401, 1415
StyD4I CCNGG 2 cut(s) 101, 672
StyI CCWWGG 3 cut(s) 366, 535, 1362
TaaI ACNGT 4 cut(s) 773, 935, 1117, 1321
TaiI ACGT 1 cut(s) 1379
TaqI TCGA 4 cut(s) 7, 238, 984, 1437
TasI AATT 6 cut(s) 108, 154, 307, 644, 1194, 1369
TatI WGTACW 2 cut(s) 274, 921
TfiI GAWTC 3 cut(s) 321, 865, 1358
TscAI CASTG 2 cut(s) 1186, 1324
TseFI GTSAC 2 cut(s) 814, 1315
TseI GCWGC 7 cut(s) 23, 584, 1048, 1280, 1283, 1425, 1445
Tsp45I GTSAC 2 cut(s) 814, 1315
TspDTI ATGAA 3 cut(s) 239, 579, 816
TspGWI ACGGA 1 cut(s) 395
TspRI CASTG 2 cut(s) 1186, 1324
VpaK11BI GGWCC 1 cut(s) 314
VspI ATTAAT 1 cut(s) 1248
XceI RCATGY 2 cut(s) 23, 80
XmiI GTMKAC 1 cut(s) 1220
XmnI GAANNNNTTC 1 cut(s) 875
XspI CTAG 6 cut(s) 204, 287, 318, 344, 1401, 1415
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.