pycom16g13010

Removal of H(2)O(2), oxidation of toxic reductants, biosynthesis and degradation of lignin, suberization, auxin catabolism, response to environmental stresses such as wounding, pathogen attack and oxidative stress

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0013299
Physical Location & Seq
Reverse (-)
9251503 .. 9252495
993 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 540 bp
ATGTTCCATGATTGCCAAGTTCAGGGCCGTGATGCTTCAATTCTTGTGGATCCGAATGCGAACATTGTGCCATCAGAGATGGCCGCAGGGAAAAACTTCGGCATCCGAAAGAGGGAGTCGATAAACATTCTTAAATCAATGGTTGAATTACAATGTCCCCAACAGGTATCTTGTGCTGACATTCTTGTGTTGGCGGCTCGAGAAGCTGTGGTTGTGTCAGGAGGGCCACAAATAAAGGTTGCATTGGGAAGGAGAGATTCCCCTGTTGCTCCAAGCTACAAGCTTGCAGATTCCTTGCTTCCTTCTCCCACTATTGGAGTTGATGGCATGCTTCAATTATTTGCAAAAAATGGAATGACAGTTGAAGAATCTGTGGCCATCATGGAGTGTTGCGTTTCTGTAGGTGCGCACACGCTAGGAGTCACGCACTGTTTGAACGTCCTAAATCGTCTAAACCAAGCAGACGAAGGCGGCCAAGTACAAGGCATGACTCCTGGGTTTGAAGCGTTTTTAAGGCTCAACTACAAGCTTTGTTCTTAA

Protein Analysis

180

Amino Acids

19.11

Weight (kDa)

5.89

Isoelectric Point (pI)

45.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
peroxidase PF00141 1 - 170 4.1e-41 Peroxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013299)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G17070 AT3G17070 AT3G42570
fragaria_vesca FvH4_4g19850
malus_domestica MD13G1152800.v1.1 MD16G1153200.v1.1
prunus_persica Prupe.1G116500_v2.0.a1 Prupe.1G116500_v2.0.a1 Prupe.1G116500_v2.0.a1
pyrus_communis pycom13g13040 pycom16g13010
rosa_chinensis RchiOBHm_Chr4g0425421
rosa_laevigata RLG00000007370
rosa_roxburghii Rroxscaffold_5G00367380
rosa_rugosa Rorug04G0202800
rosa_samantha Rh4CG277200 Rh4DG261900
rosa_wichuraiana Rw4G022510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 408
AciI CCGC 3 cut(s) 84, 194, 471
AclWI GGATC 2 cut(s) 44, 57
AcoI YGGCCR 3 cut(s) 81, 375, 472
AfaI GTAC 1 cut(s) 480
AfiI CCNNNNNNNGG 3 cut(s) 22, 112, 314
AgsI TTSAA 6 cut(s) 39, 146, 335, 365, 436, 503
AjnI CCWGG 1 cut(s) 493
AluBI AGCT 4 cut(s) 206, 276, 283, 529
AluI AGCT 4 cut(s) 206, 276, 283, 529
AlwI GGATC 2 cut(s) 44, 57
Ama87I CYCGRG 1 cut(s) 198
AoxI GGCC 5 cut(s) 25, 81, 224, 375, 472
Asp700I GAANNNNTTC 1 cut(s) 95
AspLEI GCGC 1 cut(s) 409
AspS9I GGNCC 2 cut(s) 25, 224
AvaI CYCGRG 1 cut(s) 198
BalI TGGCCA 1 cut(s) 377
BamHI GGATCC 1 cut(s) 49
BccI CCATC 4 cut(s) 73, 79, 317, 386
BceAI ACGGC 1 cut(s) 12
BcgI CGANNNNNNTGC 2 cut(s) 49, 83
BciT130I CCWGG 1 cut(s) 495
BfaI CTAG 1 cut(s) 416
BfmI CTRYAG 1 cut(s) 399
BisI GCNGC 3 cut(s) 84, 195, 472
BlsI GCNGC 3 cut(s) 85, 196, 473
Bme1390I CCNGG 1 cut(s) 495
BmeT110I CYCGRG 1 cut(s) 198
BmgT120I GGNCC 2 cut(s) 25, 224
BmiI GGNNCC 1 cut(s) 51
BmrFI CCNGG 1 cut(s) 495
BmsI GCATC 2 cut(s) 22, 111
BsaJI CCNNGG 1 cut(s) 494
Bsc4I CCNNNNNNNGG 3 cut(s) 22, 112, 314
BseBI CCWGG 1 cut(s) 495
BseDI CCNNGG 1 cut(s) 494
BseGI GGATG 1 cut(s) 102
BseLI CCNNNNNNNGG 3 cut(s) 22, 112, 314
BshFI GGCC 5 cut(s) 27, 83, 226, 377, 474
BsiHKCI CYCGRG 1 cut(s) 198
BslFI GGGAC 1 cut(s) 141
BslI CCNNNNNNNGG 3 cut(s) 22, 112, 314
BsmFI GGGAC 1 cut(s) 141
BsmI GAATGC 1 cut(s) 61
BsnI GGCC 5 cut(s) 27, 83, 226, 377, 474
BsoBI CYCGRG 1 cut(s) 198
Bsp143I GATC 1 cut(s) 49
BspACI CCGC 3 cut(s) 84, 194, 471
BspANI GGCC 5 cut(s) 27, 83, 226, 377, 474
BspLI GGNNCC 1 cut(s) 51
BspPI GGATC 2 cut(s) 44, 57
BssECI CCNNGG 1 cut(s) 494
BssMI GATC 1 cut(s) 49
Bst2UI CCWGG 1 cut(s) 495
Bst4CI ACNGT 2 cut(s) 361, 431
BstC8I GCNNGC 2 cut(s) 285, 329
BstF5I GGATG 1 cut(s) 102
BstHHI GCGC 1 cut(s) 409
BstKTI GATC 1 cut(s) 52
BstMBI GATC 1 cut(s) 49
BstMWI GCNNNNNNNGC 1 cut(s) 203
BstNI CCWGG 1 cut(s) 495
BstNSI RCATGY 1 cut(s) 331
BstSCI CCNGG 1 cut(s) 493
BstSFI CTRYAG 1 cut(s) 399
BstX2I RGATCY 1 cut(s) 49
BstYI RGATCY 1 cut(s) 49
BsuRI GGCC 5 cut(s) 27, 83, 226, 377, 474
BtsCI GGATG 1 cut(s) 102
BtsIMutI CAGTG 1 cut(s) 427
Cac8I GCNNGC 2 cut(s) 285, 329
CfoI GCGC 1 cut(s) 409
Cfr13I GGNCC 2 cut(s) 25, 224
Csp6I GTAC 1 cut(s) 479
CspCI CAANNNNNGTGG 2 cut(s) 27, 62
CviAII CATG 4 cut(s) 8, 328, 382, 487
CviQI GTAC 1 cut(s) 479
DpnI GATC 1 cut(s) 51
DpnII GATC 1 cut(s) 49
EaeI YGGCCR 3 cut(s) 81, 375, 472
Eco88I CYCGRG 1 cut(s) 198
EcoRII CCWGG 1 cut(s) 493
FaeI CATG 4 cut(s) 11, 331, 385, 490
FaiI YATR 4 cut(s) 9, 329, 383, 488
FaqI GGGAC 1 cut(s) 141
FatI CATG 4 cut(s) 7, 327, 381, 486
Fnu4HI GCNGC 3 cut(s) 84, 195, 472
FokI GGATG 1 cut(s) 89
Fsp4HI GCNGC 3 cut(s) 84, 195, 472
FspAI RTGCGCAY 1 cut(s) 408
FspBI CTAG 1 cut(s) 416
FspI TGCGCA 1 cut(s) 408
GlaI GCGC 1 cut(s) 408
GluI GCNGC 3 cut(s) 84, 195, 472
HaeIII GGCC 5 cut(s) 27, 83, 226, 377, 474
HhaI GCGC 1 cut(s) 409
Hin1II CATG 4 cut(s) 11, 331, 385, 490
Hin6I GCGC 1 cut(s) 407
HinP1I GCGC 1 cut(s) 407
HindIII AAGCTT 2 cut(s) 281, 527
HinfI GANTC 6 cut(s) 116, 257, 290, 368, 420, 490
Hpy188I TCNGA 3 cut(s) 54, 76, 107
Hpy188III TCNNGA 2 cut(s) 200, 219
HpyAV CCTTC 3 cut(s) 243, 312, 461
HpyCH4III ACNGT 2 cut(s) 361, 431
HpyCH4IV ACGT 1 cut(s) 438
HpyCH4V TGCA 3 cut(s) 242, 287, 344
HpyF10VI GCNNNNNNNGC 1 cut(s) 203
HpySE526I ACGT 1 cut(s) 438
Hsp92II CATG 4 cut(s) 11, 331, 385, 490
HspAI GCGC 1 cut(s) 407
Kzo9I GATC 1 cut(s) 49
LmnI GCTCC 1 cut(s) 274
LpnPI CCDG 7 cut(s) 8, 72, 149, 204, 276, 480, 507
LweI GCATC 2 cut(s) 22, 111
MaeI CTAG 1 cut(s) 416
MaeII ACGT 1 cut(s) 438
MaeIII GTNAC 1 cut(s) 421
MalI GATC 1 cut(s) 51
MboI GATC 1 cut(s) 49
MboII GAAGA 1 cut(s) 377
MflI RGATCY 1 cut(s) 49
MlsI TGGCCA 1 cut(s) 377
MluCI AATT 3 cut(s) 39, 146, 335
MluNI TGGCCA 1 cut(s) 377
MlyI GAGTC 3 cut(s) 125, 429, 484
MnlI CCTC 2 cut(s) 105, 215
Mox20I TGGCCA 1 cut(s) 377
MroXI GAANNNNTTC 1 cut(s) 95
MscI TGGCCA 1 cut(s) 377
MseI TTAA 3 cut(s) 132, 512, 538
MslI CAYNNNNRTG 1 cut(s) 185
Msp20I TGGCCA 1 cut(s) 377
MspR9I CCNGG 1 cut(s) 495
Mva1269I GAATGC 1 cut(s) 61
MvaI CCWGG 1 cut(s) 495
MwoI GCNNNNNNNGC 1 cut(s) 203
NdeII GATC 1 cut(s) 49
NlaIII CATG 4 cut(s) 11, 331, 385, 490
NlaIV GGNNCC 1 cut(s) 51
NmuCI GTSAC 1 cut(s) 421
NsbI TGCGCA 1 cut(s) 408
NspI RCATGY 1 cut(s) 331
PaeI GCATGC 1 cut(s) 331
PaeR7I CTCGAG 1 cut(s) 198
PctI GAATGC 1 cut(s) 61
PdmI GAANNNNTTC 1 cut(s) 95
PfeI GAWTC 3 cut(s) 257, 290, 368
PkrI GCNGC 3 cut(s) 85, 196, 473
PleI GAGTC 3 cut(s) 124, 428, 484
PpsI GAGTC 3 cut(s) 124, 428, 484
Psp6I CCWGG 1 cut(s) 493
PspGI CCWGG 1 cut(s) 493
PspN4I GGNNCC 1 cut(s) 51
PspPI GGNCC 2 cut(s) 25, 224
PsuI RGATCY 1 cut(s) 49
RsaI GTAC 1 cut(s) 480
RsaNI GTAC 1 cut(s) 479
RseI CAYNNNNRTG 1 cut(s) 185
SaqAI TTAA 3 cut(s) 132, 512, 538
SatI GCNGC 3 cut(s) 84, 195, 472
Sau3AI GATC 1 cut(s) 49
Sau96I GGNCC 2 cut(s) 25, 224
SchI GAGTC 3 cut(s) 125, 429, 484
ScrFI CCNGG 1 cut(s) 495
SetI ASST 8 cut(s) 168, 208, 240, 278, 285, 406, 441, 531
SfaNI GCATC 2 cut(s) 22, 111
SfcI CTRYAG 1 cut(s) 399
Sfr274I CTCGAG 1 cut(s) 198
SlaI CTCGAG 1 cut(s) 198
SmiMI CAYNNNNRTG 1 cut(s) 185
SmlI CTYRAG 1 cut(s) 198
SmoI CTYRAG 1 cut(s) 198
SphI GCATGC 1 cut(s) 331
Sse9I AATT 3 cut(s) 39, 146, 335
SsiI CCGC 3 cut(s) 84, 194, 471
SspMI CTAG 1 cut(s) 416
StyD4I CCNGG 1 cut(s) 493
TaaI ACNGT 2 cut(s) 361, 431
TaiI ACGT 1 cut(s) 441
TaqI TCGA 2 cut(s) 119, 199
TasI AATT 3 cut(s) 39, 146, 335
TatI WGTACW 1 cut(s) 478
TauI GCSGC 3 cut(s) 86, 197, 474
TfiI GAWTC 3 cut(s) 257, 290, 368
Tru1I TTAA 3 cut(s) 132, 512, 538
Tru9I TTAA 3 cut(s) 132, 512, 538
TscAI CASTG 1 cut(s) 434
TseFI GTSAC 1 cut(s) 421
Tsp45I GTSAC 1 cut(s) 421
TspRI CASTG 1 cut(s) 434
XceI RCATGY 1 cut(s) 331
XhoI CTCGAG 1 cut(s) 198
XmnI GAANNNNTTC 1 cut(s) 95
XspI CTAG 1 cut(s) 416
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.