Rw4G022510

Removal of H(2)O(2), oxidation of toxic reductants, biosynthesis and degradation of lignin, suberization, auxin catabolism, response to environmental stresses such as wounding, pathogen attack and oxidative stress

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0013299
Physical Location & Seq
Forward (+)
47879089 .. 47880309
1221 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G022510.1

Sequence Viewer

Length: 933 bp
ATGGATCCCAGAGCTGTAGTGGCAGGCATATCTCTGCTGCTATTTATCAGCATTGGGGCTAGAGGAGAGGGGCTTTCTTATAATTTCTATGAGGCCTCATGCGCACAAGTTGAGGACATTGTTAGAGCTGCCCACGGACCTATTTTTCTCTCTGATCCGACTACTCCGGCAGCTTTGTTGAGGCTCATGTTCCATGATTGCCAAGTTCAGGGCTGCGATGCTTCGATTCTTGTAGATTCTAGTGAGGGATATGCGTCGTCTGAGATGGCATCGGGGAGAAACTTTGGTGTCAGAAAGAGGGAGACAATCGGCATTCTGAAAACAATGGTTGAAGCAGAATGTCCTAAGCAGGTTTCTTGTACTGACATTCTCGTATTGGCAGCACGGGAGGCGGTGGCTATGTCAGGAGGGCCACAAATTAAGGTTCCTCTGGGAAGGAGAGACTCCATTGTTGCTCCAAGCTCTAAGCTTGCAGATTCTTTGCTTTCTGCAGCGAATATTGGTGTTGATGGAACGCTTCATGTCTTCGCAAAATTTGGGATGACAATCGAAGAATCTGTAGCCATTTTGGGTGCGCACACGCTGGGAGTAACACACTGTTCGAATGTCCTGAACCGCCTAAACGAAGCAGAAGACGGGCATATAAAAGGCACGATCACACCTGGGTTTGAAGCATTTTTAAGGCTAAACTGTCCTCAAGGATCCTTAGCATCAAACTTAACCTTTGTTCTAAATGATCCCACTACAGCTACATTCGACAACCACTACTACAGTAATGCAATCGGAGGCCACGGGGTGCTTCGAGTTGATGCTGAAATGGTGATGGACCCCCGCACAGCTCCGGCGCATTCTTCTGCCTTCGTCAAGCTCTCTAGCTCCGGCGTTCTGATTGGGGATCAAGGCGTTGTTAGAGAGAGTTGCAATGCAATATAG

Protein Analysis

310

Amino Acids

32.68

Weight (kDa)

5.45

Isoelectric Point (pI)

41.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
peroxidase PF00141 48 - 281 7.1e-61 Peroxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013299)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G17070 AT3G17070 AT3G42570
fragaria_vesca FvH4_4g19850
malus_domestica MD13G1152800.v1.1 MD16G1153200.v1.1
prunus_persica Prupe.1G116500_v2.0.a1 Prupe.1G116500_v2.0.a1 Prupe.1G116500_v2.0.a1
pyrus_communis pycom13g13040 pycom16g13010
rosa_chinensis RchiOBHm_Chr4g0425421
rosa_laevigata RLG00000007370
rosa_roxburghii Rroxscaffold_5G00367380
rosa_rugosa Rorug04G0202800
rosa_samantha Rh4CG277200 Rh4DG261900
rosa_wichuraiana Rw4G022510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 81
Acc16I TGCGCA 2 cut(s) 103, 576
Acc36I ACCTGC 1 cut(s) 340
AciI CCGC 3 cut(s) 392, 616, 832
AclWI GGATC 6 cut(s) 12, 149, 696, 709, 731, 903
AcsI RAATTY 1 cut(s) 533
AdeI CACNNNGTG 1 cut(s) 796
AfaI GTAC 1 cut(s) 361
AfiI CCNNNNNNNGG 1 cut(s) 208
AgsI TTSAA 2 cut(s) 332, 671
AjnI CCWGG 1 cut(s) 661
AluBI AGCT 9 cut(s) 14, 128, 173, 462, 469, 749, 839, 868, 876
AluI AGCT 9 cut(s) 14, 128, 173, 462, 469, 749, 839, 868, 876
Alw26I GTCTC 2 cut(s) 296, 435
AlwI GGATC 6 cut(s) 12, 149, 696, 709, 731, 903
AoxI GGCC 3 cut(s) 93, 410, 787
ApeKI GCWGC 6 cut(s) 37, 128, 170, 213, 380, 491
ApoI RAATTY 1 cut(s) 533
AspLEI GCGC 3 cut(s) 104, 577, 847
AspS9I GGNCC 3 cut(s) 137, 410, 826
AsuHPI GGTGA 1 cut(s) 832
AsuII TTCGAA 1 cut(s) 602
AvaII GGWCC 2 cut(s) 137, 826
BamHI GGATCC 2 cut(s) 4, 701
BbsI GAAGAC 2 cut(s) 517, 639
BbvI GCAGC 6 cut(s) 24, 115, 182, 200, 392, 503
BccI CCATC 3 cut(s) 259, 503, 817
BciT130I CCWGG 1 cut(s) 663
BcoDI GTCTC 2 cut(s) 296, 435
BfaI CTAG 3 cut(s) 60, 240, 873
BfmI CTRYAG 5 cut(s) 15, 489, 558, 744, 769
BfuAI ACCTGC 1 cut(s) 340
BisI GCNGC 6 cut(s) 38, 129, 171, 214, 381, 492
BlsI GCNGC 6 cut(s) 39, 130, 172, 215, 382, 493
Bme1390I CCNGG 1 cut(s) 663
Bme18I GGWCC 2 cut(s) 137, 826
BmgT120I GGNCC 3 cut(s) 137, 410, 826
BmiI GGNNCC 4 cut(s) 6, 426, 703, 828
BmrFI CCNGG 1 cut(s) 663
BmsI GCATC 4 cut(s) 208, 278, 719, 799
BpiI GAAGAC 2 cut(s) 517, 639
Bpu10I CCTNAGC 2 cut(s) 345, 706
Bpu14I TTCGAA 1 cut(s) 602
BpuEI CTTGAG 1 cut(s) 681
BsaBI GATNNNNATC 1 cut(s) 545
BsaJI CCNNGG 3 cut(s) 133, 662, 790
Bsc4I CCNNNNNNNGG 1 cut(s) 208
Bse3DI GCAATG 1 cut(s) 928
Bse8I GATNNNNATC 1 cut(s) 545
BseBI CCWGG 1 cut(s) 663
BseDI CCNNGG 3 cut(s) 133, 662, 790
BseGI GGATG 1 cut(s) 546
BseJI GATNNNNATC 1 cut(s) 545
BseLI CCNNNNNNNGG 1 cut(s) 208
BseMI GCAATG 1 cut(s) 928
BseMII CTCAG 1 cut(s) 252
BseRI GAGGAG 1 cut(s) 78
BseXI GCAGC 6 cut(s) 24, 115, 182, 200, 392, 503
BseYI CCCAGC 1 cut(s) 583
BshFI GGCC 3 cut(s) 95, 412, 789
BsiSI CCGG 3 cut(s) 167, 842, 879
BslI CCNNNNNNNGG 1 cut(s) 208
BsmAI GTCTC 2 cut(s) 296, 435
BsmI GAATGC 2 cut(s) 312, 847
BsnI GGCC 3 cut(s) 95, 412, 789
Bsp119I TTCGAA 1 cut(s) 602
Bsp143I GATC 6 cut(s) 4, 154, 654, 701, 736, 895
BspACI CCGC 3 cut(s) 392, 616, 832
BspANI GGCC 3 cut(s) 95, 412, 789
BspCNI CTCAG 1 cut(s) 253
BspLI GGNNCC 4 cut(s) 6, 426, 703, 828
BspMAI CTGCAG 1 cut(s) 493
BspMI ACCTGC 1 cut(s) 340
BspPI GGATC 6 cut(s) 12, 149, 696, 709, 731, 903
BspT104I TTCGAA 1 cut(s) 602
BsrDI GCAATG 1 cut(s) 928
BssECI CCNNGG 3 cut(s) 133, 662, 790
BssMI GATC 6 cut(s) 4, 154, 654, 701, 736, 895
Bst2UI CCWGG 1 cut(s) 663
Bst4CI ACNGT 3 cut(s) 599, 692, 773
BstBI TTCGAA 1 cut(s) 602
BstC8I GCNNGC 2 cut(s) 25, 471
BstDEI CTNAG 4 cut(s) 261, 345, 465, 706
BstDSI CCRYGG 2 cut(s) 133, 790
BstF5I GGATG 1 cut(s) 546
BstHHI GCGC 3 cut(s) 104, 577, 847
BstKTI GATC 6 cut(s) 7, 157, 657, 704, 739, 898
BstMAI GTCTC 2 cut(s) 296, 435
BstMBI GATC 6 cut(s) 4, 154, 654, 701, 736, 895
BstMWI GCNNNNNNNGC 3 cut(s) 20, 101, 389
BstNI CCWGG 1 cut(s) 663
BstSCI CCNGG 1 cut(s) 661
BstSFI CTRYAG 5 cut(s) 15, 489, 558, 744, 769
BstV1I GCAGC 6 cut(s) 24, 115, 182, 200, 392, 503
BstV2I GAAGAC 2 cut(s) 517, 639
BstX2I RGATCY 2 cut(s) 4, 701
BstYI RGATCY 2 cut(s) 4, 701
BsuRI GGCC 3 cut(s) 95, 412, 789
BtgI CCRYGG 2 cut(s) 133, 790
BtgZI GCGATG 1 cut(s) 231
BtsCI GGATG 1 cut(s) 546
BtsIMutI CAGTG 1 cut(s) 595
BveI ACCTGC 1 cut(s) 340
Cac8I GCNNGC 2 cut(s) 25, 471
CfoI GCGC 3 cut(s) 104, 577, 847
Cfr13I GGNCC 3 cut(s) 137, 410, 826
CseI GACGC 1 cut(s) 243
Csp6I GTAC 1 cut(s) 360
CviAII CATG 4 cut(s) 99, 187, 194, 521
CviQI GTAC 1 cut(s) 360
DdeI CTNAG 4 cut(s) 261, 345, 465, 706
DpnI GATC 6 cut(s) 6, 156, 656, 703, 738, 897
DpnII GATC 6 cut(s) 4, 154, 654, 701, 736, 895
DraIII CACNNNGTG 1 cut(s) 796
Eco147I AGGCCT 1 cut(s) 95
Eco47I GGWCC 2 cut(s) 137, 826
EcoRII CCWGG 1 cut(s) 661
FaeI CATG 4 cut(s) 102, 190, 197, 524
FatI CATG 4 cut(s) 98, 186, 193, 520
FauI CCCGC 1 cut(s) 839
Fnu4HI GCNGC 6 cut(s) 38, 129, 171, 214, 381, 492
FokI GGATG 1 cut(s) 553
Fsp4HI GCNGC 6 cut(s) 38, 129, 171, 214, 381, 492
FspAI RTGCGCAY 2 cut(s) 103, 576
FspBI CTAG 3 cut(s) 60, 240, 873
FspI TGCGCA 2 cut(s) 103, 576
GlaI GCGC 3 cut(s) 103, 576, 846
GluI GCNGC 6 cut(s) 38, 129, 171, 214, 381, 492
GsaI CCCAGC 1 cut(s) 587
HaeIII GGCC 3 cut(s) 95, 412, 789
HapII CCGG 3 cut(s) 167, 842, 879
HgaI GACGC 1 cut(s) 243
HhaI GCGC 3 cut(s) 104, 577, 847
Hin1II CATG 4 cut(s) 102, 190, 197, 524
Hin6I GCGC 3 cut(s) 102, 575, 845
HinP1I GCGC 3 cut(s) 102, 575, 845
HindIII AAGCTT 1 cut(s) 467
HinfI GANTC 5 cut(s) 226, 236, 443, 476, 554
HpaII CCGG 3 cut(s) 167, 842, 879
HphI GGTGA 1 cut(s) 832
Hpy188I TCNGA 7 cut(s) 154, 159, 262, 293, 318, 785, 888
Hpy188III TCNNGA 2 cut(s) 405, 610
Hpy99I CGWCG 1 cut(s) 259
HpyAV CCTTC 2 cut(s) 429, 868
HpyCH4III ACNGT 3 cut(s) 599, 692, 773
HpyCH4V TGCA 5 cut(s) 473, 491, 779, 921, 926
HpyF10VI GCNNNNNNNGC 3 cut(s) 20, 101, 389
HpyF3I CTNAG 4 cut(s) 261, 345, 465, 706
Hsp92II CATG 4 cut(s) 102, 190, 197, 524
HspAI GCGC 3 cut(s) 102, 575, 845
Kzo9I GATC 6 cut(s) 4, 154, 654, 701, 736, 895
LmnI GCTCC 3 cut(s) 460, 844, 881
Lsp1109I GCAGC 6 cut(s) 24, 115, 182, 200, 392, 503
LweI GCATC 4 cut(s) 208, 278, 719, 799
MaeI CTAG 3 cut(s) 60, 240, 873
MaeIII GTNAC 1 cut(s) 589
MalI GATC 6 cut(s) 6, 156, 656, 703, 738, 897
MboI GATC 6 cut(s) 4, 154, 654, 701, 736, 895
MboII GAAGA 4 cut(s) 517, 563, 644, 843
MflI RGATCY 2 cut(s) 4, 701
MluCI AATT 3 cut(s) 82, 417, 533
MlyI GAGTC 1 cut(s) 437
MmeI TCCRAC 1 cut(s) 182
MseI TTAA 3 cut(s) 420, 680, 719
MspI CCGG 3 cut(s) 167, 842, 879
MspR9I CCNGG 1 cut(s) 663
Mva1269I GAATGC 2 cut(s) 312, 847
MvaI CCWGG 1 cut(s) 663
MwoI GCNNNNNNNGC 3 cut(s) 20, 101, 389
NdeII GATC 6 cut(s) 4, 154, 654, 701, 736, 895
NlaIII CATG 4 cut(s) 102, 190, 197, 524
NlaIV GGNNCC 4 cut(s) 6, 426, 703, 828
NsbI TGCGCA 2 cut(s) 103, 576
NspV TTCGAA 1 cut(s) 602
PceI AGGCCT 1 cut(s) 95
PctI GAATGC 2 cut(s) 312, 847
PfeI GAWTC 4 cut(s) 226, 236, 476, 554
PkrI GCNGC 6 cut(s) 39, 130, 172, 215, 382, 493
PleI GAGTC 1 cut(s) 437
PpsI GAGTC 1 cut(s) 437
PsiI TTATAA 1 cut(s) 81
Psp6I CCWGG 1 cut(s) 661
PspFI CCCAGC 1 cut(s) 583
PspGI CCWGG 1 cut(s) 661
PspN4I GGNNCC 4 cut(s) 6, 426, 703, 828
PspPI GGNCC 3 cut(s) 137, 410, 826
PstI CTGCAG 1 cut(s) 493
PsuI RGATCY 2 cut(s) 4, 701
RsaI GTAC 1 cut(s) 361
RsaNI GTAC 1 cut(s) 360
SaqAI TTAA 3 cut(s) 420, 680, 719
SatI GCNGC 6 cut(s) 38, 129, 171, 214, 381, 492
Sau3AI GATC 6 cut(s) 4, 154, 654, 701, 736, 895
Sau96I GGNCC 3 cut(s) 137, 410, 826
SchI GAGTC 1 cut(s) 437
ScrFI CCNGG 1 cut(s) 663
SfaNI GCATC 4 cut(s) 208, 278, 719, 799
SfcI CTRYAG 5 cut(s) 15, 489, 558, 744, 769
SfuI TTCGAA 1 cut(s) 602
SinI GGWCC 2 cut(s) 137, 826
SmlI CTYRAG 1 cut(s) 696
SmoI CTYRAG 1 cut(s) 696
Sse9I AATT 3 cut(s) 82, 417, 533
SseBI AGGCCT 1 cut(s) 95
SsiI CCGC 3 cut(s) 392, 616, 832
SspI AATATT 1 cut(s) 499
SspMI CTAG 3 cut(s) 60, 240, 873
StuI AGGCCT 1 cut(s) 95
StyD4I CCNGG 1 cut(s) 661
TaaI ACNGT 3 cut(s) 599, 692, 773
TaqI TCGA 5 cut(s) 224, 549, 602, 756, 802
TasI AATT 3 cut(s) 82, 417, 533
TatI WGTACW 1 cut(s) 359
TfiI GAWTC 4 cut(s) 226, 236, 476, 554
Tru1I TTAA 3 cut(s) 420, 680, 719
Tru9I TTAA 3 cut(s) 420, 680, 719
TscAI CASTG 1 cut(s) 602
TseI GCWGC 6 cut(s) 37, 128, 170, 213, 380, 491
TspDTI ATGAA 1 cut(s) 509
TspGWI ACGGA 1 cut(s) 150
TspRI CASTG 1 cut(s) 602
VpaK11BI GGWCC 2 cut(s) 137, 826
XapI RAATTY 1 cut(s) 533
XcmI CCANNNNNNNNNTGG 1 cut(s) 16
XspI CTAG 3 cut(s) 60, 240, 873
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.