Rh4CG277200

Removal of H(2)O(2), oxidation of toxic reductants, biosynthesis and degradation of lignin, suberization, auxin catabolism, response to environmental stresses such as wounding, pathogen attack and oxidative stress

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0013299
Physical Location & Seq
Forward (+)
53327022 .. 53328612
1591 bp
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UTR
Exon/CDS
Intron
Rh4CG277200.1

Sequence Viewer

Length: 582 bp
ATGGGTCCCAGAGCTGTAGTGGCAGGCATATCTCTGCTGCTATTTATCAGCATTGGGGCTAGAGGAGAGGGGCTTTCTTATAATTTCTATGAGGCCTCATGCGCACAAGTTGAGGACACTGTTAGAGCTACCCTCGGACCTATTTTTCTCTCTGATCCGACTACTCCGGCAGCTTTGTTGAGGCTCAGGTTCCATGATTGCCAAGTTCAGGGCTGCGATGCTTCGATTCTTGTAGATTCTAGTGAGGGATATGCGTCTTCTGAGATGGCATCGGGGAGAAACTTTGGTGTCAGAAAGAGGGAGACAATCGGCATTCTGAAAACAATGGTTGAAGCAGAATGTCCTAAGCAGGTTTCTTGTACTGACATTCTTGTATTGGCGGCACGGGAGGCGGTGGCTATGTCAGGAGGGCCAGAAATTAAGGTTCCTCTGGGAAGGAGAGACTCCATTGTTGCTCCAAGCTCTAAGCTTGCAGATTCTTTGCTTTCTGCAGCGAATATTGGTGTTGATGGAACGCTTCATGTCTTCGCAAAATTTGGGATGACAATCGAAGAATCTGTAGCCATTTTGGGTACTACATGA

Protein Analysis

193

Amino Acids

20.25

Weight (kDa)

5.22

Isoelectric Point (pI)

47.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
peroxidase PF00141 41 - 191 3.3e-38 Peroxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013299)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G17070 AT3G17070 AT3G42570
fragaria_vesca FvH4_4g19850
malus_domestica MD13G1152800.v1.1 MD16G1153200.v1.1
prunus_persica Prupe.1G116500_v2.0.a1 Prupe.1G116500_v2.0.a1 Prupe.1G116500_v2.0.a1
pyrus_communis pycom13g13040 pycom16g13010
rosa_chinensis RchiOBHm_Chr4g0425421
rosa_laevigata RLG00000007370
rosa_roxburghii Rroxscaffold_5G00367380
rosa_rugosa Rorug04G0202800
rosa_samantha Rh4CG277200 Rh4DG261900
rosa_wichuraiana Rw4G022510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 81
Acc16I TGCGCA 1 cut(s) 103
Acc36I ACCTGC 1 cut(s) 340
AciI CCGC 2 cut(s) 380, 392
AclWI GGATC 1 cut(s) 149
AcsI RAATTY 1 cut(s) 533
AfaI GTAC 2 cut(s) 361, 574
AfiI CCNNNNNNNGG 1 cut(s) 208
AgsI TTSAA 1 cut(s) 332
AluBI AGCT 5 cut(s) 14, 128, 173, 462, 469
AluI AGCT 5 cut(s) 14, 128, 173, 462, 469
Alw26I GTCTC 2 cut(s) 296, 435
AlwI GGATC 1 cut(s) 149
AoxI GGCC 2 cut(s) 93, 410
ApeKI GCWGC 4 cut(s) 37, 170, 213, 491
ApoI RAATTY 1 cut(s) 533
AspLEI GCGC 1 cut(s) 104
AspS9I GGNCC 3 cut(s) 5, 137, 410
AvaII GGWCC 2 cut(s) 5, 137
BbsI GAAGAC 2 cut(s) 249, 517
BbvI GCAGC 4 cut(s) 24, 182, 200, 503
BccI CCATC 2 cut(s) 259, 503
BcoDI GTCTC 2 cut(s) 296, 435
BfaI CTAG 2 cut(s) 60, 240
BfmI CTRYAG 3 cut(s) 15, 489, 558
BfuAI ACCTGC 1 cut(s) 340
BisI GCNGC 5 cut(s) 38, 171, 214, 381, 492
BlsI GCNGC 5 cut(s) 39, 172, 215, 382, 493
Bme18I GGWCC 2 cut(s) 5, 137
BmgT120I GGNCC 3 cut(s) 5, 137, 410
BmiI GGNNCC 4 cut(s) 6, 7, 191, 426
BmsI GCATC 2 cut(s) 208, 278
BpiI GAAGAC 2 cut(s) 249, 517
BplI GAGNNNNNCTC 2 cut(s) 117, 149
Bpu10I CCTNAGC 2 cut(s) 185, 345
BsaBI GATNNNNATC 1 cut(s) 545
BsaJI CCNNGG 1 cut(s) 133
Bsc4I CCNNNNNNNGG 1 cut(s) 208
Bse8I GATNNNNATC 1 cut(s) 545
BseDI CCNNGG 1 cut(s) 133
BseGI GGATG 1 cut(s) 546
BseJI GATNNNNATC 1 cut(s) 545
BseLI CCNNNNNNNGG 1 cut(s) 208
BseMII CTCAG 2 cut(s) 199, 252
BseRI GAGGAG 1 cut(s) 78
BseXI GCAGC 4 cut(s) 24, 182, 200, 503
BshFI GGCC 2 cut(s) 95, 412
BsiSI CCGG 1 cut(s) 167
BslI CCNNNNNNNGG 1 cut(s) 208
BsmAI GTCTC 2 cut(s) 296, 435
BsmI GAATGC 1 cut(s) 312
BsnI GGCC 2 cut(s) 95, 412
Bsp143I GATC 1 cut(s) 154
BspACI CCGC 2 cut(s) 380, 392
BspANI GGCC 2 cut(s) 95, 412
BspCNI CTCAG 2 cut(s) 198, 253
BspLI GGNNCC 4 cut(s) 6, 7, 191, 426
BspMAI CTGCAG 1 cut(s) 493
BspMI ACCTGC 1 cut(s) 340
BspPI GGATC 1 cut(s) 149
BssECI CCNNGG 1 cut(s) 133
BssMI GATC 1 cut(s) 154
Bst4CI ACNGT 1 cut(s) 121
BstC8I GCNNGC 2 cut(s) 25, 471
BstDEI CTNAG 4 cut(s) 185, 261, 345, 465
BstF5I GGATG 1 cut(s) 546
BstHHI GCGC 1 cut(s) 104
BstKTI GATC 1 cut(s) 157
BstMAI GTCTC 2 cut(s) 296, 435
BstMBI GATC 1 cut(s) 154
BstMWI GCNNNNNNNGC 3 cut(s) 20, 101, 389
BstSFI CTRYAG 3 cut(s) 15, 489, 558
BstV1I GCAGC 4 cut(s) 24, 182, 200, 503
BstV2I GAAGAC 2 cut(s) 249, 517
BsuRI GGCC 2 cut(s) 95, 412
BtgZI GCGATG 1 cut(s) 231
BtsCI GGATG 1 cut(s) 546
BtsIMutI CAGTG 1 cut(s) 117
BveI ACCTGC 1 cut(s) 340
Cac8I GCNNGC 2 cut(s) 25, 471
CfoI GCGC 1 cut(s) 104
Cfr13I GGNCC 3 cut(s) 5, 137, 410
CseI GACGC 1 cut(s) 243
Csp6I GTAC 2 cut(s) 360, 573
CviAII CATG 4 cut(s) 99, 194, 521, 579
CviQI GTAC 2 cut(s) 360, 573
DdeI CTNAG 4 cut(s) 185, 261, 345, 465
DpnI GATC 1 cut(s) 156
DpnII GATC 1 cut(s) 154
Eco147I AGGCCT 1 cut(s) 95
Eco47I GGWCC 2 cut(s) 5, 137
EcoO109I RGGNCCY 1 cut(s) 5
FaeI CATG 4 cut(s) 102, 197, 524, 582
FaiI YATR 9 cut(s) 29, 81, 90, 100, 195, 252, 401, 522, 580
FatI CATG 4 cut(s) 98, 193, 520, 578
Fnu4HI GCNGC 5 cut(s) 38, 171, 214, 381, 492
FokI GGATG 1 cut(s) 553
Fsp4HI GCNGC 5 cut(s) 38, 171, 214, 381, 492
FspAI RTGCGCAY 1 cut(s) 103
FspBI CTAG 2 cut(s) 60, 240
FspI TGCGCA 1 cut(s) 103
GlaI GCGC 1 cut(s) 103
GluI GCNGC 5 cut(s) 38, 171, 214, 381, 492
HaeIII GGCC 2 cut(s) 95, 412
HapII CCGG 1 cut(s) 167
HgaI GACGC 1 cut(s) 243
HhaI GCGC 1 cut(s) 104
Hin1II CATG 4 cut(s) 102, 197, 524, 582
Hin6I GCGC 1 cut(s) 102
HinP1I GCGC 1 cut(s) 102
HindIII AAGCTT 1 cut(s) 467
HinfI GANTC 5 cut(s) 226, 236, 443, 476, 554
HpaII CCGG 1 cut(s) 167
Hpy188I TCNGA 6 cut(s) 137, 154, 159, 262, 293, 318
Hpy188III TCNNGA 1 cut(s) 405
HpyAV CCTTC 1 cut(s) 429
HpyCH4III ACNGT 1 cut(s) 121
HpyCH4V TGCA 2 cut(s) 473, 491
HpyF10VI GCNNNNNNNGC 3 cut(s) 20, 101, 389
HpyF3I CTNAG 4 cut(s) 185, 261, 345, 465
Hsp92II CATG 4 cut(s) 102, 197, 524, 582
HspAI GCGC 1 cut(s) 102
KflI GGGWCCC 1 cut(s) 5
Kzo9I GATC 1 cut(s) 154
LmnI GCTCC 1 cut(s) 460
LpnPI CCDG 9 cut(s) 9, 22, 172, 180, 194, 335, 390, 416, 426
Lsp1109I GCAGC 4 cut(s) 24, 182, 200, 503
LweI GCATC 2 cut(s) 208, 278
MaeI CTAG 2 cut(s) 60, 240
MalI GATC 1 cut(s) 156
MboI GATC 1 cut(s) 154
MboII GAAGA 3 cut(s) 249, 517, 563
MluCI AATT 3 cut(s) 82, 417, 533
MlyI GAGTC 1 cut(s) 437
MmeI TCCRAC 1 cut(s) 182
MseI TTAA 1 cut(s) 420
MspI CCGG 1 cut(s) 167
Mva1269I GAATGC 1 cut(s) 312
MwoI GCNNNNNNNGC 3 cut(s) 20, 101, 389
NdeII GATC 1 cut(s) 154
NlaIII CATG 4 cut(s) 102, 197, 524, 582
NlaIV GGNNCC 4 cut(s) 6, 7, 191, 426
NsbI TGCGCA 1 cut(s) 103
PceI AGGCCT 1 cut(s) 95
PctI GAATGC 1 cut(s) 312
PfeI GAWTC 4 cut(s) 226, 236, 476, 554
PkrI GCNGC 5 cut(s) 39, 172, 215, 382, 493
PleI GAGTC 1 cut(s) 437
PpsI GAGTC 1 cut(s) 437
PpuMI RGGWCCY 1 cut(s) 5
PsiI TTATAA 1 cut(s) 81
Psp5II RGGWCCY 1 cut(s) 5
PspN4I GGNNCC 4 cut(s) 6, 7, 191, 426
PspPI GGNCC 3 cut(s) 5, 137, 410
PspPPI RGGWCCY 1 cut(s) 5
PstI CTGCAG 1 cut(s) 493
RsaI GTAC 2 cut(s) 361, 574
RsaNI GTAC 2 cut(s) 360, 573
SaqAI TTAA 1 cut(s) 420
SatI GCNGC 5 cut(s) 38, 171, 214, 381, 492
Sau3AI GATC 1 cut(s) 154
Sau96I GGNCC 3 cut(s) 5, 137, 410
SchI GAGTC 1 cut(s) 437
SetI ASST 9 cut(s) 16, 130, 142, 175, 191, 354, 426, 464, 471
SfaNI GCATC 2 cut(s) 208, 278
SfcI CTRYAG 3 cut(s) 15, 489, 558
SinI GGWCC 2 cut(s) 5, 137
Sse9I AATT 3 cut(s) 82, 417, 533
SseBI AGGCCT 1 cut(s) 95
SsiI CCGC 2 cut(s) 380, 392
SspI AATATT 1 cut(s) 499
SspMI CTAG 2 cut(s) 60, 240
StuI AGGCCT 1 cut(s) 95
TaaI ACNGT 1 cut(s) 121
TaqI TCGA 2 cut(s) 224, 549
TasI AATT 3 cut(s) 82, 417, 533
TatI WGTACW 1 cut(s) 359
TauI GCSGC 1 cut(s) 383
TfiI GAWTC 4 cut(s) 226, 236, 476, 554
Tru1I TTAA 1 cut(s) 420
Tru9I TTAA 1 cut(s) 420
TscAI CASTG 1 cut(s) 124
TseI GCWGC 4 cut(s) 37, 170, 213, 491
TspDTI ATGAA 1 cut(s) 509
TspRI CASTG 1 cut(s) 124
VpaK11BI GGWCC 2 cut(s) 5, 137
XapI RAATTY 1 cut(s) 533
XcmI CCANNNNNNNNNTGG 1 cut(s) 16
XspI CTAG 2 cut(s) 60, 240
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.