RchiOBHm_Chr4g0425421

Removal of H(2)O(2), oxidation of toxic reductants, biosynthesis and degradation of lignin, suberization, auxin catabolism, response to environmental stresses such as wounding, pathogen attack and oxidative stress

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0013299
Physical Location & Seq
Forward (+)
51014447 .. 51015752
1306 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ39457

Sequence Viewer

Length: 975 bp
ATGGATCCCAGAGCTGTAGTGGCAGGCATATCTCTGCTGCTATTTATCAGCATTGGGGCTAGAGGAGAGGGGCTTTCTTATAATTTCTATGAGGCCTCATGCGCACAAGTTGAGGACATTGTTAGAGCTGCCCTCGGACCTATTTTTCTCTCTGATCCGACTACTCCGGCAGCTTTGTTGAGGCTCATGTTCCATGATTGCCAAGTTCAGGGCTGCGATGCTTCGATTCTTGTAGATTCTAGTGAGGGATATGCTTCTTCTGAGATGGCATCGGGGAGAAACTTTGGTGTCAGAAAGAGGGAGACAATCGGCATTCTGAAAACAATGGTTGAAGCAGAATGTCCTAAGCAGGTTTCTTGTACTGACATTCTTGTATTGGCGGCACGGGAGGCGGTGGCTATGTCAGGAGGGCCAGAAATTAAGGTTCCTCTGGGAAGGAGAGACTCCATTGTTGCTCCAAGCTCTAAGCTTGCAGATTCTTTGCTTTCTGCAGCGAATATTGGTGTTGATGGAACGCTTCATGTCTTCGCAAAATTTGGGATGACAATCGAAGAATCTGTAGCCATTTTGGGTGCGCACACGCTGGGAGTAACACACTGTTCGAATGTCCTGAACCGCCTAAACGAAGCAGAAGACGGGCATATAAAAGGCACGATCACACCTGGGTTTGAAGCATTTTTAAGGCTAAACTGTCCTCAAGGGTCCTTGGCATCAAACTTAACCTTTGTTCTTAATGATCCCACTACAGCTACATTCGACAACCACTACTACAGTAATGCAATCGGAGGCCACGGGGTGCTTCGAGTTGATGCTGAAATGGTGATGGACCCCCGCACAGCTCCGGCGGTTCAACAATTTGCTGAAAATGAGGATTATTTCTTTGAAGCATTTTCTTCTGCCTTCGTCAAGCTCTCTAGCTCCGGCGTTCTGATTGGGGATCAAGGCGTTGTTAGAGAGAGTTGCAATGCAATATAG

Protein Analysis

324

Amino Acids

34.38

Weight (kDa)

4.89

Isoelectric Point (pI)

42.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
peroxidase PF00141 42 - 289 1.5e-63 Peroxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013299)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G17070 AT3G17070 AT3G42570
fragaria_vesca FvH4_4g19850
malus_domestica MD13G1152800.v1.1 MD16G1153200.v1.1
prunus_persica Prupe.1G116500_v2.0.a1 Prupe.1G116500_v2.0.a1 Prupe.1G116500_v2.0.a1
pyrus_communis pycom13g13040 pycom16g13010
rosa_chinensis RchiOBHm_Chr4g0425421
rosa_laevigata RLG00000007370
rosa_roxburghii Rroxscaffold_5G00367380
rosa_rugosa Rorug04G0202800
rosa_samantha Rh4CG277200 Rh4DG261900
rosa_wichuraiana Rw4G022510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 81
Acc16I TGCGCA 2 cut(s) 103, 576
Acc36I ACCTGC 1 cut(s) 340
AciI CCGC 5 cut(s) 380, 392, 616, 832, 845
AclWI GGATC 4 cut(s) 12, 149, 731, 945
AcsI RAATTY 1 cut(s) 533
AdeI CACNNNGTG 1 cut(s) 796
AfaI GTAC 1 cut(s) 361
AfiI CCNNNNNNNGG 1 cut(s) 208
AgsI TTSAA 4 cut(s) 332, 671, 851, 884
AjnI CCWGG 1 cut(s) 661
AluBI AGCT 9 cut(s) 14, 128, 173, 462, 469, 749, 839, 910, 918
AluI AGCT 9 cut(s) 14, 128, 173, 462, 469, 749, 839, 910, 918
Alw26I GTCTC 2 cut(s) 296, 435
AlwI GGATC 4 cut(s) 12, 149, 731, 945
AoxI GGCC 3 cut(s) 93, 410, 787
ApeKI GCWGC 5 cut(s) 37, 128, 170, 213, 491
ApoI RAATTY 1 cut(s) 533
AspLEI GCGC 2 cut(s) 104, 577
AspS9I GGNCC 4 cut(s) 137, 410, 702, 826
AsuHPI GGTGA 1 cut(s) 832
AsuII TTCGAA 1 cut(s) 602
AvaII GGWCC 3 cut(s) 137, 702, 826
BamHI GGATCC 1 cut(s) 4
BbsI GAAGAC 2 cut(s) 517, 639
BbvI GCAGC 5 cut(s) 24, 115, 182, 200, 503
BccI CCATC 3 cut(s) 259, 503, 817
BciT130I CCWGG 1 cut(s) 663
BcoDI GTCTC 2 cut(s) 296, 435
BfaI CTAG 3 cut(s) 60, 240, 915
BfmI CTRYAG 5 cut(s) 15, 489, 558, 744, 769
BfuAI ACCTGC 1 cut(s) 340
BisI GCNGC 6 cut(s) 38, 129, 171, 214, 381, 492
BlsI GCNGC 6 cut(s) 39, 130, 172, 215, 382, 493
Bme1390I CCNGG 1 cut(s) 663
Bme18I GGWCC 3 cut(s) 137, 702, 826
BmgT120I GGNCC 4 cut(s) 137, 410, 702, 826
BmiI GGNNCC 4 cut(s) 6, 426, 703, 828
BmrFI CCNGG 1 cut(s) 663
BmsI GCATC 4 cut(s) 208, 278, 719, 799
BpiI GAAGAC 2 cut(s) 517, 639
BplI GAGNNNNNCTC 2 cut(s) 117, 149
Bpu10I CCTNAGC 1 cut(s) 345
Bpu14I TTCGAA 1 cut(s) 602
BpuEI CTTGAG 1 cut(s) 681
BsaBI GATNNNNATC 1 cut(s) 545
BsaJI CCNNGG 4 cut(s) 133, 662, 705, 790
Bsc4I CCNNNNNNNGG 1 cut(s) 208
Bse3DI GCAATG 1 cut(s) 970
Bse8I GATNNNNATC 1 cut(s) 545
BseBI CCWGG 1 cut(s) 663
BseDI CCNNGG 4 cut(s) 133, 662, 705, 790
BseGI GGATG 1 cut(s) 546
BseJI GATNNNNATC 1 cut(s) 545
BseLI CCNNNNNNNGG 1 cut(s) 208
BseMI GCAATG 1 cut(s) 970
BseMII CTCAG 1 cut(s) 252
BseRI GAGGAG 1 cut(s) 78
BseXI GCAGC 5 cut(s) 24, 115, 182, 200, 503
BseYI CCCAGC 1 cut(s) 583
BshFI GGCC 3 cut(s) 95, 412, 789
BsiSI CCGG 3 cut(s) 167, 842, 921
BslI CCNNNNNNNGG 1 cut(s) 208
BsmAI GTCTC 2 cut(s) 296, 435
BsmI GAATGC 1 cut(s) 312
BsnI GGCC 3 cut(s) 95, 412, 789
Bsp119I TTCGAA 1 cut(s) 602
Bsp143I GATC 5 cut(s) 4, 154, 654, 736, 937
BspACI CCGC 5 cut(s) 380, 392, 616, 832, 845
BspANI GGCC 3 cut(s) 95, 412, 789
BspCNI CTCAG 1 cut(s) 253
BspLI GGNNCC 4 cut(s) 6, 426, 703, 828
BspMAI CTGCAG 1 cut(s) 493
BspMI ACCTGC 1 cut(s) 340
BspPI GGATC 4 cut(s) 12, 149, 731, 945
BspT104I TTCGAA 1 cut(s) 602
BsrDI GCAATG 1 cut(s) 970
BssECI CCNNGG 4 cut(s) 133, 662, 705, 790
BssMI GATC 5 cut(s) 4, 154, 654, 736, 937
BssT1I CCWWGG 1 cut(s) 705
Bst2UI CCWGG 1 cut(s) 663
Bst4CI ACNGT 3 cut(s) 599, 692, 773
BstBI TTCGAA 1 cut(s) 602
BstC8I GCNNGC 2 cut(s) 25, 471
BstDEI CTNAG 3 cut(s) 261, 345, 465
BstDSI CCRYGG 1 cut(s) 790
BstF5I GGATG 1 cut(s) 546
BstHHI GCGC 2 cut(s) 104, 577
BstKTI GATC 5 cut(s) 7, 157, 657, 739, 940
BstMAI GTCTC 2 cut(s) 296, 435
BstMBI GATC 5 cut(s) 4, 154, 654, 736, 937
BstMWI GCNNNNNNNGC 3 cut(s) 20, 101, 389
BstNI CCWGG 1 cut(s) 663
BstSCI CCNGG 1 cut(s) 661
BstSFI CTRYAG 5 cut(s) 15, 489, 558, 744, 769
BstV1I GCAGC 5 cut(s) 24, 115, 182, 200, 503
BstV2I GAAGAC 2 cut(s) 517, 639
BstX2I RGATCY 1 cut(s) 4
BstYI RGATCY 1 cut(s) 4
BsuRI GGCC 3 cut(s) 95, 412, 789
BtgI CCRYGG 1 cut(s) 790
BtgZI GCGATG 1 cut(s) 231
BtsCI GGATG 1 cut(s) 546
BtsIMutI CAGTG 1 cut(s) 595
BveI ACCTGC 1 cut(s) 340
Cac8I GCNNGC 2 cut(s) 25, 471
CfoI GCGC 2 cut(s) 104, 577
Cfr13I GGNCC 4 cut(s) 137, 410, 702, 826
Csp6I GTAC 1 cut(s) 360
CviAII CATG 4 cut(s) 99, 187, 194, 521
CviQI GTAC 1 cut(s) 360
DdeI CTNAG 3 cut(s) 261, 345, 465
DpnI GATC 5 cut(s) 6, 156, 656, 738, 939
DpnII GATC 5 cut(s) 4, 154, 654, 736, 937
DraIII CACNNNGTG 1 cut(s) 796
Eco130I CCWWGG 1 cut(s) 705
Eco147I AGGCCT 1 cut(s) 95
Eco47I GGWCC 3 cut(s) 137, 702, 826
EcoO109I RGGNCCY 1 cut(s) 702
EcoRII CCWGG 1 cut(s) 661
EcoT14I CCWWGG 1 cut(s) 705
ErhI CCWWGG 1 cut(s) 705
FaeI CATG 4 cut(s) 102, 190, 197, 524
FatI CATG 4 cut(s) 98, 186, 193, 520
FauI CCCGC 1 cut(s) 839
Fnu4HI GCNGC 6 cut(s) 38, 129, 171, 214, 381, 492
FokI GGATG 1 cut(s) 553
Fsp4HI GCNGC 6 cut(s) 38, 129, 171, 214, 381, 492
FspAI RTGCGCAY 2 cut(s) 103, 576
FspBI CTAG 3 cut(s) 60, 240, 915
FspI TGCGCA 2 cut(s) 103, 576
GlaI GCGC 2 cut(s) 103, 576
GluI GCNGC 6 cut(s) 38, 129, 171, 214, 381, 492
GsaI CCCAGC 1 cut(s) 587
HaeIII GGCC 3 cut(s) 95, 412, 789
HapII CCGG 3 cut(s) 167, 842, 921
HhaI GCGC 2 cut(s) 104, 577
Hin1II CATG 4 cut(s) 102, 190, 197, 524
Hin6I GCGC 2 cut(s) 102, 575
HinP1I GCGC 2 cut(s) 102, 575
HindIII AAGCTT 1 cut(s) 467
HinfI GANTC 5 cut(s) 226, 236, 443, 476, 554
HpaII CCGG 3 cut(s) 167, 842, 921
HphI GGTGA 1 cut(s) 832
Hpy188I TCNGA 8 cut(s) 137, 154, 159, 262, 293, 318, 785, 930
Hpy188III TCNNGA 2 cut(s) 405, 610
HpyAV CCTTC 2 cut(s) 429, 910
HpyCH4III ACNGT 3 cut(s) 599, 692, 773
HpyCH4V TGCA 5 cut(s) 473, 491, 779, 963, 968
HpyF10VI GCNNNNNNNGC 3 cut(s) 20, 101, 389
HpyF3I CTNAG 3 cut(s) 261, 345, 465
Hsp92II CATG 4 cut(s) 102, 190, 197, 524
HspAI GCGC 2 cut(s) 102, 575
Kzo9I GATC 5 cut(s) 4, 154, 654, 736, 937
LmnI GCTCC 3 cut(s) 460, 844, 923
Lsp1109I GCAGC 5 cut(s) 24, 115, 182, 200, 503
LweI GCATC 4 cut(s) 208, 278, 719, 799
MaeI CTAG 3 cut(s) 60, 240, 915
MaeIII GTNAC 1 cut(s) 589
MalI GATC 5 cut(s) 6, 156, 656, 738, 939
MboI GATC 5 cut(s) 4, 154, 654, 736, 937
MboII GAAGA 5 cut(s) 249, 517, 563, 644, 885
MflI RGATCY 1 cut(s) 4
MluCI AATT 4 cut(s) 82, 417, 533, 854
MlyI GAGTC 1 cut(s) 437
MmeI TCCRAC 1 cut(s) 182
MseI TTAA 4 cut(s) 420, 680, 719, 732
MspI CCGG 3 cut(s) 167, 842, 921
MspR9I CCNGG 1 cut(s) 663
Mva1269I GAATGC 1 cut(s) 312
MvaI CCWGG 1 cut(s) 663
MwoI GCNNNNNNNGC 3 cut(s) 20, 101, 389
NdeII GATC 5 cut(s) 4, 154, 654, 736, 937
NlaIII CATG 4 cut(s) 102, 190, 197, 524
NlaIV GGNNCC 4 cut(s) 6, 426, 703, 828
NsbI TGCGCA 2 cut(s) 103, 576
NspV TTCGAA 1 cut(s) 602
PceI AGGCCT 1 cut(s) 95
PctI GAATGC 1 cut(s) 312
PfeI GAWTC 4 cut(s) 226, 236, 476, 554
PkrI GCNGC 6 cut(s) 39, 130, 172, 215, 382, 493
PleI GAGTC 1 cut(s) 437
PpsI GAGTC 1 cut(s) 437
PpuMI RGGWCCY 1 cut(s) 702
PsiI TTATAA 1 cut(s) 81
Psp5II RGGWCCY 1 cut(s) 702
Psp6I CCWGG 1 cut(s) 661
PspFI CCCAGC 1 cut(s) 583
PspGI CCWGG 1 cut(s) 661
PspN4I GGNNCC 4 cut(s) 6, 426, 703, 828
PspPI GGNCC 4 cut(s) 137, 410, 702, 826
PspPPI RGGWCCY 1 cut(s) 702
PstI CTGCAG 1 cut(s) 493
PsuI RGATCY 1 cut(s) 4
RsaI GTAC 1 cut(s) 361
RsaNI GTAC 1 cut(s) 360
SaqAI TTAA 4 cut(s) 420, 680, 719, 732
SatI GCNGC 6 cut(s) 38, 129, 171, 214, 381, 492
Sau3AI GATC 5 cut(s) 4, 154, 654, 736, 937
Sau96I GGNCC 4 cut(s) 137, 410, 702, 826
SchI GAGTC 1 cut(s) 437
ScrFI CCNGG 1 cut(s) 663
SfaNI GCATC 4 cut(s) 208, 278, 719, 799
SfcI CTRYAG 5 cut(s) 15, 489, 558, 744, 769
SfuI TTCGAA 1 cut(s) 602
SinI GGWCC 3 cut(s) 137, 702, 826
SmlI CTYRAG 1 cut(s) 696
SmoI CTYRAG 1 cut(s) 696
Sse9I AATT 4 cut(s) 82, 417, 533, 854
SseBI AGGCCT 1 cut(s) 95
SsiI CCGC 5 cut(s) 380, 392, 616, 832, 845
SspI AATATT 1 cut(s) 499
SspMI CTAG 3 cut(s) 60, 240, 915
StuI AGGCCT 1 cut(s) 95
StyD4I CCNGG 1 cut(s) 661
StyI CCWWGG 1 cut(s) 705
TaaI ACNGT 3 cut(s) 599, 692, 773
TaqI TCGA 5 cut(s) 224, 549, 602, 756, 802
TasI AATT 4 cut(s) 82, 417, 533, 854
TatI WGTACW 1 cut(s) 359
TauI GCSGC 1 cut(s) 383
TfiI GAWTC 4 cut(s) 226, 236, 476, 554
Tru1I TTAA 4 cut(s) 420, 680, 719, 732
Tru9I TTAA 4 cut(s) 420, 680, 719, 732
TscAI CASTG 1 cut(s) 602
TseI GCWGC 5 cut(s) 37, 128, 170, 213, 491
TspDTI ATGAA 1 cut(s) 509
TspRI CASTG 1 cut(s) 602
VpaK11BI GGWCC 3 cut(s) 137, 702, 826
XapI RAATTY 1 cut(s) 533
XcmI CCANNNNNNNNNTGG 1 cut(s) 16
XspI CTAG 3 cut(s) 60, 240, 915
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.