Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr16

Orthogroup ID

OG0008978

Physical Location & Seq

Genomic Coordinates Forward (+)

10082730 .. 10087818

Gene Structure 5089 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2106 bp

ATGGGCAGCAGCGGAAAGGGCCGGACAGTTCTGGAGGTGGGAGCCGACGGCGTCGCTCTCATCACCATCATCAACCCACCTGTCAATTCCCTCTCGTTCGATGTGTTGTACAGCTTGAAAGACAGTTATGAGGAGGCCTTGCGAAGAAATGATGTGAAGGCAATTGTTATAACAGGTGCTAAAGGAAAATTTTCGGGTGGTTTCGATATTTCTGCTTTCGGTGGACTCCAAGGGGGGCAGAAGCAGGAGCAAAAGCTCGGTTTTATATCCGTGGAGGTAATCACTGACATTTTTGAAGGTGCAAGGAAACCGTCAGTTGCCGCCATTGATGGCCTTGCACTGGGGGGAGGTTTGGAGGTGGCAATGGCATGTCATGCCCGAATTTCGACTCCTACTGCACAATTAGGGTTGCCCGAGCTGCAGCTTGGACTCATTCCCGGTTTTGGAGGAACGCAACGGCTTCCACGTCTTGTTGGAATCTCAAAGGCACTTGAGATGATGCTGTTGGTAAGCACTGCACGTAAGTGGGTGTTGGATATCTTGGAGCGTAGAAGACCTTGGGTTGCTAGTCTTCACAAAACCGACAAGTTAGAGCCCCTTGGAGAAGCTAGGGAAATACTTAAATTTGCAAGAGCTCAAGTCCGGAAGCAGGCTCCCAATCTCAAACACCCATTGGTTTGCATTGATGTTGTTGAGGAGGGTATAGCTTCTGGGGGTCGGTCTGGACTTTTGAAGGAGGCTGAATCATTTCAAGGACTTCTGCAGTCAGACACTTGCAAGAGCTTGGTCCATGTCTTCTTTGCTCAACGTGGAACATCAAGGGTACCTGGGGTTACTGATCGTGGTTTGATGCCTAGACGAGTGACTAAGGTTGCTGTCATTGGTGGAGGGTTAATGGGGTCTGGAATAACTACCGCATTGATTCTTAGTGGTTATCCAGCGATCCTGAAAGAAGTAAACGATAAGTTCTTGCAGGCTGGGATTGGTAGAGTCAGAGCCAATCTGCAGAGCCGTGTCAAGAAAGGGAAAATGACTCAAGAAAAGTTTGAGAAGACCGTCTCTCTTCTTAAGGGTGTCCTGGATTATGAAAACTTTAGAGATGTAGATATGGTCATAGAGGCCGTTATTGAGAATGTTTCTCTAAAGCAGCAGATTTTTGCTGATCTTGAAAAATATTGCCCACAACACTGCATACTTGCAAGTAATACTTCCACAATCGACTTGAATCTCATTGGAGAGAAGACCAAGTCTCATGATCGGATTATTGGTGCCCATTTCTTTAGCCCGGCACATGTCATGCCACTTCTGGAAATTGTTCGTACTAATCAGACATCTCCCCAAGTTATTGTTGATTTGCTAGAAGTTGGGAAGAGAATAAAGAAAACACCAGTTGTTGTTGGGAATTGCACAGGATTTGCTGTGAACAGAATGTTCTTTCCTTATACGCAAGCTGCTCTCTTACTTGTTGAACGAGGTGCTGATGTTTATCAGATTGATATGGTAATCACCAAATTCGGAATGCCTATGGGCCCATTCAGATTGGTTGATCTGGTTGGGTTTGGCGTGGCAATTGCAACTGGCATGCAATTTGTTGTGAACTTTCCTGAGCGGACATATAAATCAATGCTTATCCCCTTGATGCAGGAGGACGGGAGGGCAGGTGAAACTACTCGAAAAGGGTTCTATACTTATGATGATAGGCGCAAAGCTAGCCCAGATCCTCAATTGAAAGGATATATTGAGAAGTCAAGAAGCATTTCGGGTGTTTCCATAGATCCCAAGCTACTGAAGTTATCAGAACAGGACATAGTGGAGATGATATTTTTCCCTGTTGTAAATGAGGCATGCCGGGTGCTTGCTGAAGGTATTGCTGTAAAAGCTGCTGACCTTGACATTGCTGCAATCATGGGAATGGGTTTCCCACCATACAGGGGAGGTCTCTTGTTCTGGGCTGATTTACTTGGATCCAAGTACATATATTCAAGATTGGAGGAATGGTCAAATACTTATGGTGAATTTTTCAAGCCTTGCGCCTATTTGGCTGAAAGAGCTGCAAACGGGGCTCCTCTGAGTTCTCACTCGAACCAAGCAAAATCTCGGTTATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004165 GO:0004300 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0006082 GO:0006629 GO:0006631 GO:0006635 GO:0008150 GO:0008152 GO:0008692 GO:0009056 GO:0009062 GO:0009514 GO:0009987 GO:0016042 GO:0016054 GO:0016829 GO:0016835 GO:0016836 GO:0016853 GO:0016854 GO:0016856 GO:0016860 GO:0016863 GO:0018812 GO:0019395 GO:0019752 GO:0030258 GO:0032787 GO:0034440 GO:0042579 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044242 GO:0044248 GO:0044255 GO:0044281 GO:0044282 GO:0044424 GO:0044444 GO:0044464 GO:0046395 GO:0055114 GO:0071704 GO:0072329 GO:1901575

KEGG Pathways

Metabolic & Signaling

ko00071 ko00592 ko01100 ko01110 ko01212 map00071 map00592 map01100 map01110 map01212

Pfam Domains

Protein Families

3HCDH 3HCDH_N ECH_1

Protein Analysis

702

Amino Acids

76.53

Weight (kDa)

9.19

Isoelectric Point (pI)

32.89

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ECH_1	PF00378	16 - 170	8.9e-30	Enoyl-CoA hydratase/isomerase
ECH_2	PF16113	28 - 159	4e-19	Enoyl-CoA hydratase/isomerase
3HCDH_N	PF02737	290 - 468	1.5e-60	3-hydroxyacyl-CoA dehydrogenase, NAD binding domain
3HCDH	PF00725	471 - 564	1e-20	3-hydroxyacyl-CoA dehydrogenase, C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008978)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G06860
fragaria_vesca	FvH4_4g18500 FvH4_4g18500 FvH4_4g18500
malus_domestica	MD13G1166700.v1.1 MD16G1166400.v1.1
prunus_persica	Prupe.1G133000_v2.0.a1
pyrus_communis	pycom13g14220 pycom16g14060
rosa_chinensis	RchiOBHm_Chr4g0423051
rosa_laevigata	RLG00000007590
rosa_multiflora	Rmu_co8121856.1_g000001 Rmu_sc0006639.1_g000001 Rmu_sc0023965.1_g000001 Rmu_sc0028464.1_g000006
rosa_roxburghii	Rroxscaffold_5G00365130
rosa_rugosa	Rorug04G0183300.1
rosa_samantha	Rh4AG243100 Rh4BG247800 Rh4CG257600 Rh4DG242800
rosa_wichuraiana	Rw4G020970

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	170, 2104
AarI	CACCTGC	1 cut(s)	1649
Acc36I	ACCTGC	1 cut(s)	1649
Acc65I	GGTACC	1 cut(s)	823
AccB1I	GGYRCC	2 cut(s)	823, 1268
AccBSI	CCGCTC	1 cut(s)	1609
AccIII	TCCGGA	1 cut(s)	642
AciI	CCGC	4 cut(s)	12, 321, 915, 1609
AclWI	GGATC	5 cut(s)	937, 1712, 1769, 1959, 1972
AcsI	RAATTY	5 cut(s)	188, 381, 623, 1511, 2015
AcuI	CTGAAG	2 cut(s)	1808, 1881
AcyI	GRCGYC	1 cut(s)	51
AfaI	GTAC	4 cut(s)	110, 825, 1321, 1973
AfiI	CCNNNNNNNGG	4 cut(s)	340, 443, 649, 1931
AflII	CTTAAG	1 cut(s)	1067
AflIII	ACRYGT	1 cut(s)	1291
AjiI	CACGTC	1 cut(s)	467
AjnI	CCWGG	2 cut(s)	826, 1077
AleI	CACNNNNGTG	1 cut(s)	523
Alw21I	GWGCWC	1 cut(s)	637
Alw26I	GTCTC	3 cut(s)	1063, 1254, 1943
AlwI	GGATC	5 cut(s)	937, 1712, 1769, 1959, 1972
Ama87I	CYCGRG	1 cut(s)	413
Aor13HI	TCCGGA	1 cut(s)	642
AoxI	GGCC	5 cut(s)	19, 135, 331, 1119, 1528
ApaI	GGGCCC	1 cut(s)	1532
ApeKI	GCWGC	9 cut(s)	6, 9, 418, 421, 1147, 1451, 1880, 1898, 2051
ApoI	RAATTY	5 cut(s)	188, 381, 623, 1511, 2015
Asp700I	GAANNNNTTC	4 cut(s)	190, 747, 1314, 1756
Asp718I	GGTACC	1 cut(s)	823
AspLEI	GCGC	2 cut(s)	1704, 2033
AspS9I	GGNCC	4 cut(s)	19, 787, 1528, 1529
AsuC2I	CCSGG	3 cut(s)	438, 1286, 1850
AsuHPI	GGTGA	4 cut(s)	55, 1498, 1673, 2024
AsuNHI	GCTAGC	1 cut(s)	1709
AvaI	CYCGRG	1 cut(s)	413
AvaII	GGWCC	1 cut(s)	787
BaeGI	GKGCMC	2 cut(s)	1273, 1532
BamHI	GGATCC	1 cut(s)	1964
BanI	GGYRCC	2 cut(s)	823, 1268
BanII	GRGCYC	4 cut(s)	597, 637, 1532, 2065
BbsI	GAAGAC	5 cut(s)	559, 563, 787, 1058, 1247
Bbv12I	GWGCWC	1 cut(s)	637
BbvI	GCAGC	9 cut(s)	18, 21, 405, 433, 1159, 1438, 1867, 1885, 2038
BccI	CCATC	2 cut(s)	74, 323
BceAI	ACGGC	4 cut(s)	64, 473, 996, 1106
BcgI	CGANNNNNNTGC	2 cut(s)	194, 228
BciT130I	CCWGG	2 cut(s)	828, 1079
BcnI	CCSGG	3 cut(s)	438, 1286, 1850
BcoDI	GTCTC	3 cut(s)	1063, 1254, 1943
BfaI	CTAG	5 cut(s)	567, 609, 855, 1358, 1710
BfmI	CTRYAG	3 cut(s)	419, 761, 1004
BfrI	CTTAAG	1 cut(s)	1067
BfuAI	ACCTGC	1 cut(s)	1649
BglI	GCCNNNNNGGC	1 cut(s)	2039
Bme1390I	CCNGG	5 cut(s)	438, 828, 1079, 1286, 1850
Bme18I	GGWCC	1 cut(s)	787
BmeT110I	CYCGRG	1 cut(s)	413
BmgBI	CACGTC	1 cut(s)	467
BmgT120I	GGNCC	4 cut(s)	19, 787, 1528, 1529
BmiI	GGNNCC	7 cut(s)	43, 654, 825, 1270, 1530, 1966, 2064
BmrFI	CCNGG	5 cut(s)	438, 828, 1079, 1286, 1850
BmrI	ACTGGG	1 cut(s)	350
BmsI	GCATC	3 cut(s)	489, 840, 1629
BmtI	GCTAGC	1 cut(s)	1713
BmuI	ACTGGG	1 cut(s)	350
BpiI	GAAGAC	5 cut(s)	559, 563, 787, 1058, 1247
BpmI	CTGGAG	1 cut(s)	53
Bpu10I	CCTNAGC	1 cut(s)	1605
BpuEI	CTTGAG	3 cut(s)	512, 621, 1020
BpuMI	CCSGG	3 cut(s)	438, 1286, 1850
BsaAI	YACGTR	1 cut(s)	521
BsaBI	GATNNNNATC	1 cut(s)	1485
BsaHI	GRCGYC	1 cut(s)	51
BsaI	GGTCTC	1 cut(s)	1943
BsaJI	CCNNGG	5 cut(s)	229, 270, 557, 598, 827
BsaWI	WCCGGW	1 cut(s)	642
BsaXI	ACNNNNNCTCC	4 cut(s)	1645, 1675, 1982, 2012
Bsc4I	CCNNNNNNNGG	4 cut(s)	340, 443, 649, 1931
Bse1I	ACTGG	3 cut(s)	345, 1388, 1582
Bse3DI	GCAATG	2 cut(s)	369, 1893
Bse8I	GATNNNNATC	1 cut(s)	1485
BseAI	TCCGGA	1 cut(s)	642
BseBI	CCWGG	2 cut(s)	828, 1079
BseDI	CCNNGG	5 cut(s)	229, 270, 557, 598, 827
BseJI	GATNNNNATC	1 cut(s)	1485
BseLI	CCNNNNNNNGG	4 cut(s)	340, 443, 649, 1931
BseMI	GCAATG	2 cut(s)	369, 1893
BseMII	CTCAG	2 cut(s)	1596, 2060
BseNI	ACTGG	3 cut(s)	345, 1388, 1582
BseRI	GAGGAG	3 cut(s)	146, 710, 2055
BseSI	GKGCMC	2 cut(s)	1273, 1532
BseXI	GCAGC	9 cut(s)	18, 21, 405, 433, 1159, 1438, 1867, 1885, 2038
BseYI	CCCAGC	1 cut(s)	977
BsgI	GTGCAG	2 cut(s)	381, 501
BshFI	GGCC	5 cut(s)	21, 137, 333, 1121, 1530
BshNI	GGYRCC	2 cut(s)	823, 1268
BsiHKAI	GWGCWC	1 cut(s)	637
BsiHKCI	CYCGRG	1 cut(s)	413
BsiSI	CCGG	5 cut(s)	22, 438, 643, 1286, 1849
BslI	CCNNNNNNNGG	4 cut(s)	340, 443, 649, 1931
BsmAI	GTCTC	3 cut(s)	1063, 1254, 1943
BsmBI	CGTCTC	1 cut(s)	1063
BsmI	GAATGC	1 cut(s)	1524
BsnI	GGCC	5 cut(s)	21, 137, 333, 1121, 1530
Bso31I	GGTCTC	1 cut(s)	1943
BsoBI	CYCGRG	1 cut(s)	413
Bsp120I	GGGCCC	1 cut(s)	1528
Bsp1286I	GDGCHC	5 cut(s)	597, 637, 1273, 1532, 2065
Bsp13I	TCCGGA	1 cut(s)	642
Bsp1407I	TGTACA	1 cut(s)	108
Bsp143I	GATC	8 cut(s)	838, 942, 1162, 1255, 1546, 1717, 1774, 1964
BspACI	CCGC	4 cut(s)	12, 321, 915, 1609
BspANI	GGCC	5 cut(s)	21, 137, 333, 1121, 1530
BspCNI	CTCAG	2 cut(s)	1597, 2061
BspEI	TCCGGA	1 cut(s)	642
BspHI	TCATGA	1 cut(s)	1252
BspLI	GGNNCC	7 cut(s)	43, 654, 825, 1270, 1530, 1966, 2064
BspMAI	CTGCAG	3 cut(s)	423, 765, 1008
BspMI	ACCTGC	1 cut(s)	1649
BspOI	GCTAGC	1 cut(s)	1713
BspPI	GGATC	5 cut(s)	937, 1712, 1769, 1959, 1972
BspT107I	GGYRCC	2 cut(s)	823, 1268
BspTI	CTTAAG	1 cut(s)	1067
BspTNI	GGTCTC	1 cut(s)	1943
BsrBI	CCGCTC	1 cut(s)	1609
BsrDI	GCAATG	2 cut(s)	369, 1893
BsrGI	TGTACA	1 cut(s)	108
BsrI	ACTGG	3 cut(s)	345, 1388, 1582
BssECI	CCNNGG	5 cut(s)	229, 270, 557, 598, 827
BssMI	GATC	8 cut(s)	838, 942, 1162, 1255, 1546, 1717, 1774, 1964
BssNI	GRCGYC	1 cut(s)	51
BssT1I	CCWWGG	3 cut(s)	229, 557, 598
Bst2UI	CCWGG	2 cut(s)	828, 1079
Bst4CI	ACNGT	4 cut(s)	28, 125, 312, 1057
Bst6I	CTCTTC	2 cut(s)	1068, 1364
BstACI	GRCGYC	1 cut(s)	51
BstAFI	CTTAAG	1 cut(s)	1067
BstAPI	GCANNNNNTGC	1 cut(s)	374
BstAUI	TGTACA	1 cut(s)	108
BstBAI	YACGTR	1 cut(s)	521
BstC8I	GCNNGC	7 cut(s)	651, 975, 1449, 1583, 1711, 1846, 1857
BstDEI	CTNAG	4 cut(s)	867, 926, 1605, 2069
BstDSI	CCRYGG	1 cut(s)	270
BstHHI	GCGC	2 cut(s)	1704, 2033
BstKTI	GATC	8 cut(s)	841, 945, 1165, 1258, 1549, 1720, 1777, 1967
BstMAI	GTCTC	3 cut(s)	1063, 1254, 1943
BstMBI	GATC	8 cut(s)	838, 942, 1162, 1255, 1546, 1717, 1774, 1964
BstMWI	GCNNNNNNNGC	8 cut(s)	18, 374, 418, 1710, 1877, 2039, 2048, 2060
BstNI	CCWGG	2 cut(s)	828, 1079
BstNSI	RCATGY	4 cut(s)	372, 1295, 1585, 1848
BstSCI	CCNGG	5 cut(s)	436, 826, 1077, 1284, 1848
BstSFI	CTRYAG	3 cut(s)	419, 761, 1004
BstSLI	GKGCMC	2 cut(s)	1273, 1532
BstV1I	GCAGC	9 cut(s)	18, 21, 405, 433, 1159, 1438, 1867, 1885, 2038
BstV2I	GAAGAC	5 cut(s)	559, 563, 787, 1058, 1247
BstX2I	RGATCY	3 cut(s)	1717, 1774, 1964
BstYI	RGATCY	3 cut(s)	1717, 1774, 1964
BsuRI	GGCC	5 cut(s)	21, 137, 333, 1121, 1530
BtgI	CCRYGG	1 cut(s)	270
BtrI	CACGTC	1 cut(s)	467
BtsI	GCAGTG	2 cut(s)	513, 1186
BtsIMutI	CAGTG	4 cut(s)	282, 338, 513, 1186
BveI	ACCTGC	1 cut(s)	1649
Cac8I	GCNNGC	7 cut(s)	651, 975, 1449, 1583, 1711, 1846, 1857
CciI	TCATGA	1 cut(s)	1252
CfoI	GCGC	2 cut(s)	1704, 2033
Cfr13I	GGNCC	4 cut(s)	19, 787, 1528, 1529
CseI	GACGC	1 cut(s)	40
Csp6I	GTAC	4 cut(s)	109, 824, 1320, 1972
CviAII	CATG	9 cut(s)	369, 374, 791, 1253, 1292, 1297, 1582, 1845, 1906
CviQI	GTAC	4 cut(s)	109, 824, 1320, 1972
DdeI	CTNAG	4 cut(s)	867, 926, 1605, 2069
DpnI	GATC	8 cut(s)	840, 944, 1164, 1257, 1548, 1719, 1776, 1966
DpnII	GATC	8 cut(s)	838, 942, 1162, 1255, 1546, 1717, 1774, 1964
Eam1104I	CTCTTC	2 cut(s)	1068, 1364
EarI	CTCTTC	2 cut(s)	1068, 1364
Ecl136II	GAGCTC	1 cut(s)	635
Eco130I	CCWWGG	3 cut(s)	229, 557, 598
Eco147I	AGGCCT	1 cut(s)	137
Eco24I	GRGCYC	4 cut(s)	597, 637, 1532, 2065
Eco31I	GGTCTC	1 cut(s)	1943
Eco32I	GATATC	1 cut(s)	538
Eco47I	GGWCC	1 cut(s)	787
Eco53kI	GAGCTC	1 cut(s)	635
Eco57I	CTGAAG	2 cut(s)	1808, 1881
Eco88I	CYCGRG	1 cut(s)	413
EcoICRI	GAGCTC	1 cut(s)	635
EcoRII	CCWGG	2 cut(s)	826, 1077
EcoRV	GATATC	1 cut(s)	538
EcoT14I	CCWWGG	3 cut(s)	229, 557, 598
EcoT38I	GRGCYC	4 cut(s)	597, 637, 1532, 2065
ErhI	CCWWGG	3 cut(s)	229, 557, 598
Esp3I	CGTCTC	1 cut(s)	1063
FaeI	CATG	9 cut(s)	372, 377, 794, 1256, 1295, 1300, 1585, 1848, 1909
FalI	AAGNNNNNCTT	2 cut(s)	1192, 1224
FatI	CATG	9 cut(s)	368, 373, 790, 1252, 1291, 1296, 1581, 1844, 1905
FriOI	GRGCYC	4 cut(s)	597, 637, 1532, 2065
FspBI	CTAG	5 cut(s)	567, 609, 855, 1358, 1710
GlaI	GCGC	2 cut(s)	1703, 2032
GsaI	CCCAGC	1 cut(s)	981
GsuI	CTGGAG	1 cut(s)	53
HaeIII	GGCC	5 cut(s)	21, 137, 333, 1121, 1530
HapII	CCGG	5 cut(s)	22, 438, 643, 1286, 1849
HgaI	GACGC	1 cut(s)	40
HhaI	GCGC	2 cut(s)	1704, 2033
Hin1I	GRCGYC	1 cut(s)	51
Hin1II	CATG	9 cut(s)	372, 377, 794, 1256, 1295, 1300, 1585, 1848, 1909
Hin6I	GCGC	2 cut(s)	1702, 2031
HinP1I	GCGC	2 cut(s)	1702, 2031
HinfI	GANTC	9 cut(s)	225, 388, 429, 477, 743, 922, 990, 1033, 1225
HpaII	CCGG	5 cut(s)	22, 438, 643, 1286, 1849
HphI	GGTGA	4 cut(s)	55, 1498, 1673, 2024
Hpy166II	GTNNAC	4 cut(s)	224, 958, 1423, 1597
Hpy188I	TCNGA	9 cut(s)	769, 995, 1260, 1329, 1491, 1517, 1538, 1798, 2070
Hpy8I	GTNNAC	4 cut(s)	224, 958, 1423, 1597
Hpy99I	CGWCG	2 cut(s)	50, 56
HpyAV	CCTTC	4 cut(s)	151, 290, 727, 1856
HpyCH4III	ACNGT	4 cut(s)	28, 125, 312, 1057
HpyCH4IV	ACGT	3 cut(s)	466, 520, 808
HpyF10VI	GCNNNNNNNGC	8 cut(s)	18, 374, 418, 1710, 1877, 2039, 2048, 2060
HpyF3I	CTNAG	4 cut(s)	867, 926, 1605, 2069
HpySE526I	ACGT	3 cut(s)	466, 520, 808
Hsp92I	GRCGYC	1 cut(s)	51
Hsp92II	CATG	9 cut(s)	372, 377, 794, 1256, 1295, 1300, 1585, 1848, 1909
HspAI	GCGC	2 cut(s)	1702, 2031
Kpn2I	TCCGGA	1 cut(s)	642
KpnI	GGTACC	1 cut(s)	827
Kzo9I	GATC	8 cut(s)	838, 942, 1162, 1255, 1546, 1717, 1774, 1964
LmnI	GCTCC	5 cut(s)	41, 247, 544, 658, 2068
Lsp1109I	GCAGC	9 cut(s)	18, 21, 405, 433, 1159, 1438, 1867, 1885, 2038
LweI	GCATC	3 cut(s)	489, 840, 1629
MaeI	CTAG	5 cut(s)	567, 609, 855, 1358, 1710
MaeII	ACGT	3 cut(s)	466, 520, 808
MaeIII	GTNAC	2 cut(s)	832, 862
MalI	GATC	8 cut(s)	840, 944, 1164, 1257, 1548, 1719, 1776, 1966
MbiI	CCGCTC	1 cut(s)	1609
MboI	GATC	8 cut(s)	838, 942, 1162, 1255, 1546, 1717, 1774, 1964
MboII	GAAGA	8 cut(s)	156, 563, 564, 787, 1055, 1063, 1252, 1381
MfeI	CAATTG	3 cut(s)	162, 1569, 1724
MflI	RGATCY	3 cut(s)	1717, 1774, 1964
MhlI	GDGCHC	5 cut(s)	597, 637, 1273, 1532, 2065
MlyI	GAGTC	5 cut(s)	219, 382, 423, 999, 1027
MmeI	TCCRAC	2 cut(s)	454, 513
MroI	TCCGGA	1 cut(s)	642
MroXI	GAANNNNTTC	4 cut(s)	190, 747, 1314, 1756
MseI	TTAA	3 cut(s)	621, 893, 1068
MslI	CAYNNNNRTG	2 cut(s)	523, 1910
MspA1I	CMGCKG	1 cut(s)	12
MspCI	CTTAAG	1 cut(s)	1067
MspI	CCGG	5 cut(s)	22, 438, 643, 1286, 1849
MspR9I	CCNGG	5 cut(s)	438, 828, 1079, 1286, 1850
MunI	CAATTG	3 cut(s)	162, 1569, 1724
Mva1269I	GAATGC	1 cut(s)	1524
MvaI	CCWGG	2 cut(s)	828, 1079
MwoI	GCNNNNNNNGC	8 cut(s)	18, 374, 418, 1710, 1877, 2039, 2048, 2060
NciI	CCSGG	3 cut(s)	438, 1286, 1850
NdeII	GATC	8 cut(s)	838, 942, 1162, 1255, 1546, 1717, 1774, 1964
NheI	GCTAGC	1 cut(s)	1709
NlaIII	CATG	9 cut(s)	372, 377, 794, 1256, 1295, 1300, 1585, 1848, 1909
NlaIV	GGNNCC	7 cut(s)	43, 654, 825, 1270, 1530, 1966, 2064
NmuCI	GTSAC	1 cut(s)	862
NspI	RCATGY	4 cut(s)	372, 1295, 1585, 1848
OliI	CACNNNNGTG	1 cut(s)	523
PaeI	GCATGC	2 cut(s)	1585, 1848
PagI	TCATGA	1 cut(s)	1252
PaqCI	CACCTGC	1 cut(s)	1649
PceI	AGGCCT	1 cut(s)	137
PciI	ACATGT	1 cut(s)	1291
PcsI	WCGNNNNNNNCGW	1 cut(s)	463
PctI	GAATGC	1 cut(s)	1524
PdmI	GAANNNNTTC	4 cut(s)	190, 747, 1314, 1756
PfeI	GAWTC	4 cut(s)	477, 743, 922, 1225
PflFI	GACNNNGTC	2 cut(s)	50, 1246
PfoI	TCCNGGA	1 cut(s)	1077
PleI	GAGTC	5 cut(s)	219, 382, 423, 998, 1027
PpsI	GAGTC	5 cut(s)	219, 382, 423, 998, 1027
Ppu21I	YACGTR	1 cut(s)	521
PscI	ACATGT	1 cut(s)	1291
PsiI	TTATAA	2 cut(s)	170, 2104
Psp124BI	GAGCTC	1 cut(s)	637
Psp6I	CCWGG	2 cut(s)	826, 1077
PspFI	CCCAGC	1 cut(s)	977
PspGI	CCWGG	2 cut(s)	826, 1077
PspN4I	GGNNCC	7 cut(s)	43, 654, 825, 1270, 1530, 1966, 2064
PspOMI	GGGCCC	1 cut(s)	1528
PspPI	GGNCC	4 cut(s)	19, 787, 1528, 1529
PstI	CTGCAG	3 cut(s)	423, 765, 1008
PsuI	RGATCY	3 cut(s)	1717, 1774, 1964
PsyI	GACNNNGTC	2 cut(s)	50, 1246
RsaI	GTAC	4 cut(s)	110, 825, 1321, 1973
RsaNI	GTAC	4 cut(s)	109, 824, 1320, 1972
RseI	CAYNNNNRTG	2 cut(s)	523, 1910
SacI	GAGCTC	1 cut(s)	637
SaqAI	TTAA	3 cut(s)	621, 893, 1068
Sau3AI	GATC	8 cut(s)	838, 942, 1162, 1255, 1546, 1717, 1774, 1964
Sau96I	GGNCC	4 cut(s)	19, 787, 1528, 1529
SchI	GAGTC	5 cut(s)	219, 382, 423, 999, 1027
ScrFI	CCNGG	5 cut(s)	438, 828, 1079, 1286, 1850
SduI	GDGCHC	5 cut(s)	597, 637, 1273, 1532, 2065
SfaNI	GCATC	3 cut(s)	489, 840, 1629
SfcI	CTRYAG	3 cut(s)	419, 761, 1004
SinI	GGWCC	1 cut(s)	787
SmiMI	CAYNNNNRTG	2 cut(s)	523, 1910
SmlI	CTYRAG	4 cut(s)	491, 636, 1035, 1067
SmoI	CTYRAG	4 cut(s)	491, 636, 1035, 1067
SphI	GCATGC	2 cut(s)	1585, 1848
SseBI	AGGCCT	1 cut(s)	137
SsiI	CCGC	4 cut(s)	12, 321, 915, 1609
SspI	AATATT	1 cut(s)	1175
SspMI	CTAG	5 cut(s)	567, 609, 855, 1358, 1710
SstI	GAGCTC	1 cut(s)	637
StuI	AGGCCT	1 cut(s)	137
StyD4I	CCNGG	5 cut(s)	436, 826, 1077, 1284, 1848
StyI	CCWWGG	3 cut(s)	229, 557, 598
TaaI	ACNGT	4 cut(s)	28, 125, 312, 1057
TaiI	ACGT	3 cut(s)	469, 523, 811
TaqI	TCGA	6 cut(s)	99, 204, 386, 1218, 1672, 2081
TaqII	GACCGA	1 cut(s)	708
TatI	WGTACW	2 cut(s)	108, 1971
TauI	GCSGC	1 cut(s)	323
TfiI	GAWTC	4 cut(s)	477, 743, 922, 1225
Tru1I	TTAA	3 cut(s)	621, 893, 1068
Tru9I	TTAA	3 cut(s)	621, 893, 1068
TscAI	CASTG	4 cut(s)	289, 345, 520, 1193
TseFI	GTSAC	1 cut(s)	862
TseI	GCWGC	9 cut(s)	6, 9, 418, 421, 1147, 1451, 1880, 1898, 2051
Tsp45I	GTSAC	1 cut(s)	862
TspDTI	ATGAA	1 cut(s)	1101
TspGWI	ACGGA	1 cut(s)	259
TspRI	CASTG	4 cut(s)	289, 345, 520, 1193
Tth111I	GACNNNGTC	2 cut(s)	50, 1246
Vha464I	CTTAAG	1 cut(s)	1067
VpaK11BI	GGWCC	1 cut(s)	787
XapI	RAATTY	5 cut(s)	188, 381, 623, 1511, 2015
XceI	RCATGY	4 cut(s)	372, 1295, 1585, 1848
XmnI	GAANNNNTTC	4 cut(s)	190, 747, 1314, 1756
XspI	CTAG	5 cut(s)	567, 609, 855, 1358, 1710

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.