Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr16

Orthogroup ID

OG0007310

Physical Location & Seq

Genomic Coordinates Forward (+)

10231157 .. 10240122

Gene Structure 8966 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2886 bp

ATGACAATCAAGTTTTCAAGAAAGTTGTTGGTAAAAATACAGGGGAAAATTTCATTCTTCATGATGGTCCTCCGTATGCCAATGGAGATCGTTCACATAGGCCATGCTTTAAATAAGATTTTAAAGGATTTTATCAATCGTTATAAGCTTCTTCAAAATTATAAAGTTCATTATGTGCCTGGTTGGGATTGTCATGGCTTACCAATTGAGTTGAAAGTTCTTCAATCACTTGATCATGCTTCTCGAAAGGATCTAACACCAATAAAGTTGAGAGCAAAGGCAGCCAAATTTGCCAAGCAAACCGTTAAAACTCAGATGGAATCATTTAAGCGTTATGGAGTGTGGGCAGAGTGGAATAATCCCTATCTCACTCTTGATCCAGAATATGAAGCTGCTCAGATAGAAGTGTTTGGCCAGATGGTCCTTCAAGGTTTTATCTACAGAGGCAAGAAACCAGTTCACTGGAGCCCCTCATCACGAACTGCTCTTGCAGAGGCTGAGTTGGAGTATCCGGAGGGGCATGTTTCGAGAAGCATATACACCATTTTCAAATTGGTCAGTGCCTCTCCCACTTCAGGTGGCTTATTAAATGAGTATTTCCCAGATGTGTGCTTGGCCATTTGGACAACTACTCCCTGGACTATTCCAGCCAATGCTGCTGTTGCAGTGAATGCCAAGCTTTTATATGCCATTGTTGAAGTACAGTCAGTCCCAGAAGATTCTTTATCTGATGGAAATAAAAAGGGAAGGCCTGGAAATCTTTTGAAGGAAGAAAAGAAGCCTTTCCTAATTGTCGCTTCAGATCTTGTGCCAGTGTTAGAAGCAAAATGGGGTGTGAAGCTTGTTGTTAGGAAAAGGGTGTCAGGTTCAGATCTGGAAAACTGCAGGTATGTCCATCCAATATTTAATAGGGAATGCCCGGTTGTCATTGGAGGAGATTATATTACAACGGACTCAGGAACAGGACTGGTCCATACAGCTCCTGGACATGGTCAGGAGGATTATGTCACTGGCCAGAAGTACGGACTACCTATGCTTTCTCCTGTAGATGATGATGGAAAGTTCACTGAAGAAGCTGGAAAGTTTTGTGGGCTTGATGTGCTTGCGGATGGTAATATTGCTGTTGTGAAATACTTGGATGAACATTTATCAATTATCATGGAAGAATCCTACAAACATAAGTATCCATATGATTGGAGAACAAAAAAGCCAACAATATTTAGGGCAACTGAGCAATGGTTTGCTTCAGTGGAAGGGTTTCGTGACGCTGTTATGGATGCAATTGGTCGTGTAAAATGGATTCCATCTAAGGCAGAAAACAGAATATCTGCAATGACTTCTAGCCGCTCTGATTGGTGTATATCGCGGCAGAGGACATGGGGCGTTCCAATTCCAGTCTTTTATCATGTCGATTCAAAGGAACCTCTGATGAATGAAGAGACTATTGAGTATATCAAGTCTATAATATCCGAAAAGGGTAGTGATGCGTGGTGGTACATGAAGGTGGAGGATCTCCTCCCTGATAAATATCGTGATAAAGCATCTGAATATGAAAAGGGGACTGATACAATGGATGTATGGTTTGATTCAGGCTCTTCTTGGGCTGCAGTATTGGGAAAAAGAAATAGCCATAGTCTTCCGGCAGATTTGTACCTTGAAGGTATGGATCAGCATCGTGGGTGGTTCCAGAGTTCTTTGTTAACAAGTATTGCTACTAAAGGAAAGGCTCCATATTCCAGTGTTATAACACATGGATTTGTATTGGATGAGAAAGGTTTGAAAATGAGCAAATCTTTGGGTAACGTAGTCGATCCACGGACTGTGATTGAAGGAGGCAAGAACCAAAAGGATGGCTATGGAGCCGATGTCCTGCGTCTCTGGGTTTCTAGTGTAGATTATACAGGCGATGTAACAATCGGCACCCAAATTCTCCGTCAAATGTCAGATATTTATAGGAAGATGCGAGGAACATTGAGATACCTTTTGGGAAATCTTCATGATTGGAATGCTGATAATGCTATCGCATACCATGATCTTCCCATGATTGATCAGTACGCGCTGTTTCAGCTTGAAAATTTTGTGAAGAACAGTGGAGAGTGCTATGAAAATTATCAATTCTTCAAGATATTTCAGATCATACAGCGGTTTGTTGTTGTTGACCTCTCAAATTTCTATTTTGATGTCGCCAAAGATCGCCTTTATGTTGGGGGCACAACAAGTTTTACTAGGAGAAGTTGTCAAACAGTTCTTGCAGCACTTCTCCTATCTGTAGTGAGAGTGATTGCTCCAATATTACCTCATTTGGCTGAAGATGTATGGCAAAACCTTCCATTCCAGTACACTGACGAAGATGGCTCTGCTGCTGAATTTGTTTTTGAATCAAGATGGCCAGCTTTGAACAACACATGGCTCTCTCTTTCGAAAGAGGAAACTGATTTCTGGGAAAAAGTTCTTGAGTTGAGAACTGAGGTGAATAAAGTTCTGGAGGTTGCTCGTACGGAAAAGCTAATTGGCTCTAGTTTAGATGCCAAGGTCTACCTCCACACTTCTGATTCCAGTCTGGCCTCTAGATTAGTTGAAATTTCTGCAGCCGACAACGATGCAGACACATTGAATCGAATTTTCATTACATCTCAAGCAGAGGTTCTCCCGTCCTTGAAGGAGGAGCTGATTGCGAATATACCGCACAAAGGAGAGTACCTCATCGAAGAAAACATCAAAGTCTGGATTGGAGTGTCTCGCGCCGAGGGCTTAAAGTGCGAAAGATGCTGGAATTATTCACCTCAAGTTGGTTCCTTTCCAGAGCACAACACCCTTTGCAGCCGCTGTTATAATGTGGTTGATATTCAACAGTCTCAATCTCCAGCTGTAGCTGTGGTCAGTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0007275 GO:0008150 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009791 GO:0009908 GO:0022414 GO:0032501 GO:0032502 GO:0035670 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0048367 GO:0048437 GO:0048438 GO:0048440 GO:0048467 GO:0048481 GO:0048608 GO:0048731 GO:0048827 GO:0048856 GO:0061458 GO:0090567 GO:0099402

KEGG Pathways

Metabolic & Signaling

ko00970 map00970

Pfam Domains

Protein Families

Anticodon_1 tRNA-synt_1 zf-FPG_IleRS

Protein Analysis

962

Amino Acids

108.89

Weight (kDa)

6.17

Isoelectric Point (pI)

40.17

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
tRNA-synt_1g	PF09334	30 - 169	2.2e-09	tRNA synthetases class I (M)
tRNA-synt_1	PF00133	31 - 639	2.1e-120	tRNA synthetases class I (I, L, M and V)
Anticodon_1	PF08264	683 - 846	2.9e-30	Anticodon-binding domain of tRNA ligase
zf-FPG_IleRS	PF06827	917 - 946	7.2e-08	Zinc finger found in FPG and IleRS

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007310)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G49030 AT5G49030 AT5G49030
fragaria_vesca	FvH4_4g18060 FvH4_4g18060 FvH4_4g18060 FvH4_4g18060 FvH4_4g18060 FvH4_4g18060
malus_domestica	MD13G1168400.v1.1 MD16G1168800.v1.1
prunus_persica	Prupe.1G136500_v2.0.a1 Prupe.1G136500_v2.0.a1
pyrus_communis	pycom13g14400 pycom16g14210
rosa_chinensis	RchiOBHm_Chr4g0422271
rosa_laevigata	RLG00000007623
rosa_multiflora	Rmu_sc0013245.1_g000018
rosa_roxburghii	Rroxscaffold_5G00364380
rosa_rugosa	Rorug04G0179100
rosa_samantha	Rh4AG238300 Rh4BG241400 Rh4CG253300 Rh4DG237000
rosa_wichuraiana	Rw4G020620

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	4 cut(s)	144, 162, 1745, 2832
Acc36I	ACCTGC	1 cut(s)	876
AccB1I	GGYRCC	1 cut(s)	1919
AccBSI	CCGCTC	1 cut(s)	1347
AccI	GTMKAC	1 cut(s)	2535
AccII	CGCG	3 cut(s)	1366, 2057, 2742
AccIII	TCCGGA	1 cut(s)	511
AciI	CCGC	6 cut(s)	1106, 1345, 1366, 2143, 2684, 2824
AclWI	GGATC	5 cut(s)	258, 371, 1518, 1674, 1805
AcoI	YGGCCR	4 cut(s)	412, 615, 1012, 2387
AcsI	RAATTY	8 cut(s)	48, 287, 1926, 2074, 2167, 2366, 2580, 2619
AcuI	CTGAAG	5 cut(s)	558, 783, 1089, 1230, 2328
AfaI	GTAC	8 cut(s)	702, 1022, 1496, 1652, 2054, 2339, 2497, 2699
AfiI	CCNNNNNNNGG	4 cut(s)	575, 989, 2690, 2789
AjnI	CCWGG	4 cut(s)	178, 635, 751, 982
AjuI	GAANNNNNNNTTGG	2 cut(s)	2493, 2525
Alw21I	GWGCWC	1 cut(s)	2808
Alw26I	GTCTC	4 cut(s)	1433, 1880, 2742, 2859
AlwI	GGATC	5 cut(s)	258, 371, 1518, 1674, 1805
AlwNI	CAGNNNCTG	3 cut(s)	497, 983, 2826
Aor13HI	TCCGGA	1 cut(s)	511
AoxI	GGCC	7 cut(s)	100, 412, 615, 749, 1012, 2387, 2562
ApeKI	GCWGC	8 cut(s)	281, 392, 656, 1604, 2252, 2360, 2588, 2820
ApoI	RAATTY	8 cut(s)	48, 287, 1926, 2074, 2167, 2366, 2580, 2619
ArsI	GACNNNNNNTTYG	2 cut(s)	1918, 1950
Asp700I	GAANNNNTTC	3 cut(s)	782, 1257, 2448
AspLEI	GCGC	2 cut(s)	2059, 2744
AspS9I	GGNCC	3 cut(s)	67, 421, 970
AsuC2I	CCSGG	1 cut(s)	920
AsuHPI	GGTGA	2 cut(s)	2482, 2772
AsuII	TTCGAA	1 cut(s)	2420
AvaII	GGWCC	3 cut(s)	67, 421, 970
BaeGI	GKGCMC	1 cut(s)	2213
BaeI	ACNNNNGTAYC	2 cut(s)	1166, 1199
BalI	TGGCCA	4 cut(s)	414, 617, 1014, 2389
BanI	GGYRCC	1 cut(s)	1919
BanII	GRGCYC	1 cut(s)	470
BbsI	GAAGAC	1 cut(s)	1628
Bbv12I	GWGCWC	1 cut(s)	2808
BbvI	GCAGC	8 cut(s)	293, 379, 643, 1591, 2264, 2347, 2600, 2832
BcgI	CGANNNNNNTGC	2 cut(s)	2582, 2616
BciT130I	CCWGG	4 cut(s)	180, 637, 753, 984
BciVI	GTATCC	2 cut(s)	519, 1194
BclI	TGATCA	2 cut(s)	232, 2047
BcnI	CCSGG	1 cut(s)	920
BcoDI	GTCTC	4 cut(s)	1433, 1880, 2742, 2859
BfaI	CTAG	5 cut(s)	1341, 1887, 2226, 2517, 2568
BfmI	CTRYAG	7 cut(s)	439, 883, 1044, 1605, 2268, 2586, 2868
BfuAI	ACCTGC	1 cut(s)	876
BfuI	GTATCC	2 cut(s)	519, 1194
BglII	AGATCT	2 cut(s)	802, 871
Bme1390I	CCNGG	5 cut(s)	180, 637, 753, 920, 984
Bme18I	GGWCC	3 cut(s)	67, 421, 970
BmgT120I	GGNCC	3 cut(s)	67, 421, 970
BmiI	GGNNCC	7 cut(s)	467, 1422, 1685, 1728, 1861, 1921, 2794
BmrFI	CCNGG	5 cut(s)	180, 637, 753, 920, 984
BmsI	GCATC	8 cut(s)	1267, 1474, 1550, 1681, 1950, 2515, 2590, 2756
BpiI	GAAGAC	1 cut(s)	1628
BplI	GAGNNNNNCTC	2 cut(s)	2685, 2717
BpmI	CTGGAG	3 cut(s)	484, 2504, 2847
Bpu14I	TTCGAA	1 cut(s)	2420
BpuEI	CTTGAG	3 cut(s)	2474, 2619, 2769
BpuMI	CCSGG	1 cut(s)	920
BsaBI	GATNNNNATC	2 cut(s)	1527, 1671
BsaJI	CCNNGG	4 cut(s)	635, 1814, 2529, 2745
BsaWI	WCCGGW	1 cut(s)	511
BsaXI	ACNNNNNCTCC	6 cut(s)	616, 646, 1851, 1881, 2221, 2251
Bsc4I	CCNNNNNNNGG	4 cut(s)	575, 989, 2690, 2789
Bse1I	ACTGG	9 cut(s)	455, 467, 812, 972, 1015, 1394, 1737, 2335, 2556
Bse3DI	GCAATG	2 cut(s)	1241, 1338
Bse8I	GATNNNNATC	2 cut(s)	1527, 1671
BseAI	TCCGGA	1 cut(s)	511
BseBI	CCWGG	4 cut(s)	180, 637, 753, 984
BseDI	CCNNGG	4 cut(s)	635, 1814, 2529, 2745
BseGI	GGATG	7 cut(s)	895, 1114, 1144, 1282, 1579, 1771, 1855
BseJI	GATNNNNATC	2 cut(s)	1527, 1671
BseLI	CCNNNNNNNGG	4 cut(s)	575, 989, 2690, 2789
BseMI	GCAATG	2 cut(s)	1241, 1338
BseMII	CTCAG	6 cut(s)	326, 410, 489, 969, 1221, 2457
BseNI	ACTGG	9 cut(s)	455, 467, 812, 972, 1015, 1394, 1737, 2335, 2556
BseRI	GAGGAG	3 cut(s)	948, 1505, 2678
BseSI	GKGCMC	1 cut(s)	2213
BseXI	GCAGC	8 cut(s)	293, 379, 643, 1591, 2264, 2347, 2600, 2832
Bsh1236I	CGCG	3 cut(s)	1366, 2057, 2742
BshFI	GGCC	7 cut(s)	102, 414, 617, 751, 1014, 2389, 2564
BshNI	GGYRCC	1 cut(s)	1919
BsiHKAI	GWGCWC	1 cut(s)	2808
BsiSI	CCGG	3 cut(s)	512, 920, 1640
BsiWI	CGTACG	1 cut(s)	2495
BslFI	GGGAC	2 cut(s)	695, 1573
BslI	CCNNNNNNNGG	4 cut(s)	575, 989, 2690, 2789
BsmAI	GTCTC	4 cut(s)	1433, 1880, 2742, 2859
BsmBI	CGTCTC	1 cut(s)	1880
BsmFI	GGGAC	2 cut(s)	695, 1573
BsmI	GAATGC	3 cut(s)	676, 920, 2011
BsnI	GGCC	7 cut(s)	102, 414, 617, 751, 1014, 2389, 2564
Bsp119I	TTCGAA	1 cut(s)	2420
Bsp1286I	GDGCHC	3 cut(s)	470, 2213, 2808
Bsp13I	TCCGGA	1 cut(s)	511
BspACI	CCGC	6 cut(s)	1106, 1345, 1366, 2143, 2684, 2824
BspANI	GGCC	7 cut(s)	102, 414, 617, 751, 1014, 2389, 2564
BspCNI	CTCAG	6 cut(s)	325, 409, 490, 968, 1222, 2458
BspEI	TCCGGA	1 cut(s)	511
BspFNI	CGCG	3 cut(s)	1366, 2057, 2742
BspHI	TCATGA	2 cut(s)	60, 1996
BspLI	GGNNCC	7 cut(s)	467, 1422, 1685, 1728, 1861, 1921, 2794
BspMAI	CTGCAG	3 cut(s)	887, 1609, 2590
BspMI	ACCTGC	1 cut(s)	876
BspPI	GGATC	5 cut(s)	258, 371, 1518, 1674, 1805
BspQI	GCTCTTC	1 cut(s)	1600
BspT104I	TTCGAA	1 cut(s)	2420
BspT107I	GGYRCC	1 cut(s)	1919
BsrBI	CCGCTC	1 cut(s)	1347
BsrDI	GCAATG	2 cut(s)	1241, 1338
BsrI	ACTGG	9 cut(s)	455, 467, 812, 972, 1015, 1394, 1737, 2335, 2556
BssECI	CCNNGG	4 cut(s)	635, 1814, 2529, 2745
BssT1I	CCWWGG	1 cut(s)	2529
Bst2UI	CCWGG	4 cut(s)	180, 637, 753, 984
Bst4CI	ACNGT	6 cut(s)	304, 705, 1822, 2090, 2245, 2853
Bst6I	CTCTTC	2 cut(s)	1431, 1600
BstAPI	GCANNNNNTGC	1 cut(s)	671
BstBI	TTCGAA	1 cut(s)	2420
BstC8I	GCNNGC	2 cut(s)	1104, 2391
BstDEI	CTNAG	7 cut(s)	312, 396, 498, 955, 1230, 1308, 2466
BstDSI	CCRYGG	1 cut(s)	1814
BstF5I	GGATG	7 cut(s)	895, 1114, 1144, 1282, 1579, 1771, 1855
BstFNI	CGCG	3 cut(s)	1366, 2057, 2742
BstHHI	GCGC	2 cut(s)	2059, 2744
BstMAI	GTCTC	4 cut(s)	1433, 1880, 2742, 2859
BstNI	CCWGG	4 cut(s)	180, 637, 753, 984
BstNSI	RCATGY	1 cut(s)	524
BstSCI	CCNGG	5 cut(s)	178, 635, 751, 918, 982
BstSFI	CTRYAG	7 cut(s)	439, 883, 1044, 1605, 2268, 2586, 2868
BstSLI	GKGCMC	1 cut(s)	2213
BstUI	CGCG	3 cut(s)	1366, 2057, 2742
BstV1I	GCAGC	8 cut(s)	293, 379, 643, 1591, 2264, 2347, 2600, 2832
BstV2I	GAAGAC	1 cut(s)	1628
BstX2I	RGATCY	4 cut(s)	250, 802, 871, 1510
BstXI	CCANNNNNNTGG	3 cut(s)	462, 1194, 1850
BstYI	RGATCY	4 cut(s)	250, 802, 871, 1510
BsuI	GTATCC	2 cut(s)	519, 1194
BsuRI	GGCC	7 cut(s)	102, 414, 617, 751, 1014, 2389, 2564
BtgI	CCRYGG	1 cut(s)	1814
BtgZI	GCGATG	1 cut(s)	1920
BtsCI	GGATG	7 cut(s)	895, 1114, 1144, 1282, 1579, 1771, 1855
BtsI	GCAGTG	1 cut(s)	672
BveI	ACCTGC	1 cut(s)	876
Cac8I	GCNNGC	2 cut(s)	1104, 2391
CaiI	CAGNNNCTG	3 cut(s)	497, 983, 2826
CciI	TCATGA	2 cut(s)	60, 1996
CfoI	GCGC	2 cut(s)	2059, 2744
Cfr13I	GGNCC	3 cut(s)	67, 421, 970
CseI	GACGC	2 cut(s)	1274, 1862
Csp6I	GTAC	8 cut(s)	701, 1021, 1495, 1651, 2053, 2338, 2496, 2698
CviQI	GTAC	8 cut(s)	701, 1021, 1495, 1651, 2053, 2338, 2496, 2698
DdeI	CTNAG	7 cut(s)	312, 396, 498, 955, 1230, 1308, 2466
DraI	TTTAAA	2 cut(s)	111, 123
EaeI	YGGCCR	4 cut(s)	412, 615, 1012, 2387
Eam1104I	CTCTTC	2 cut(s)	1431, 1600
EarI	CTCTTC	2 cut(s)	1431, 1600
Eco130I	CCWWGG	1 cut(s)	2529
Eco147I	AGGCCT	1 cut(s)	751
Eco24I	GRGCYC	1 cut(s)	470
Eco47I	GGWCC	3 cut(s)	67, 421, 970
Eco57I	CTGAAG	5 cut(s)	558, 783, 1089, 1230, 2328
EcoRII	CCWGG	4 cut(s)	178, 635, 751, 982
EcoT14I	CCWWGG	1 cut(s)	2529
EcoT38I	GRGCYC	1 cut(s)	470
ErhI	CCWWGG	1 cut(s)	2529
Esp3I	CGTCTC	1 cut(s)	1880
FalI	AAGNNNNNCTT	2 cut(s)	2181, 2213
FaqI	GGGAC	2 cut(s)	695, 1573
FauNDI	CATATG	1 cut(s)	1189
FbaI	TGATCA	2 cut(s)	232, 2047
FblI	GTMKAC	1 cut(s)	2535
FokI	GGATG	7 cut(s)	882, 1121, 1151, 1289, 1586, 1778, 1862
FriOI	GRGCYC	1 cut(s)	470
FspBI	CTAG	5 cut(s)	1341, 1887, 2226, 2517, 2568
GlaI	GCGC	2 cut(s)	2058, 2743
GsuI	CTGGAG	3 cut(s)	484, 2504, 2847
HaeIII	GGCC	7 cut(s)	102, 414, 617, 751, 1014, 2389, 2564
HapII	CCGG	3 cut(s)	512, 920, 1640
HgaI	GACGC	2 cut(s)	1274, 1862
HhaI	GCGC	2 cut(s)	2059, 2744
Hin6I	GCGC	2 cut(s)	2057, 2742
HinP1I	GCGC	2 cut(s)	2057, 2742
HincII	GTYRAC	2 cut(s)	1701, 2158
HindII	GTYRAC	2 cut(s)	1701, 2158
HindIII	AAGCTT	3 cut(s)	146, 677, 839
HpaI	GTTAAC	1 cut(s)	1701
HpaII	CCGG	3 cut(s)	512, 920, 1640
HphI	GGTGA	2 cut(s)	2482, 2772
Hpy166II	GTNNAC	7 cut(s)	94, 460, 1065, 1701, 2158, 2340, 2536
Hpy8I	GTNNAC	7 cut(s)	94, 460, 1065, 1701, 2158, 2340, 2536
HpyAV	CCTTC	9 cut(s)	434, 741, 760, 1247, 1495, 1652, 1823, 2336, 2653
HpyCH4III	ACNGT	6 cut(s)	304, 705, 1822, 2090, 2245, 2853
HpyCH4IV	ACGT	1 cut(s)	1803
HpyF3I	CTNAG	7 cut(s)	312, 396, 498, 955, 1230, 1308, 2466
HpySE526I	ACGT	1 cut(s)	1803
HspAI	GCGC	2 cut(s)	2057, 2742
Kpn2I	TCCGGA	1 cut(s)	511
Ksp22I	TGATCA	2 cut(s)	232, 2047
KspAI	GTTAAC	1 cut(s)	1701
LguI	GCTCTTC	1 cut(s)	1600
LmnI	GCTCC	6 cut(s)	465, 985, 1732, 1859, 2290, 2665
Lsp1109I	GCAGC	8 cut(s)	293, 379, 643, 1591, 2264, 2347, 2600, 2832
LweI	GCATC	8 cut(s)	1267, 1474, 1550, 1681, 1950, 2515, 2590, 2756
MaeI	CTAG	5 cut(s)	1341, 1887, 2226, 2517, 2568
MaeII	ACGT	1 cut(s)	1803
MaeIII	GTNAC	4 cut(s)	1006, 1262, 1799, 1909
MbiI	CCGCTC	1 cut(s)	1347
MfeI	CAATTG	2 cut(s)	204, 1281
MflI	RGATCY	4 cut(s)	250, 802, 871, 1510
MhlI	GDGCHC	3 cut(s)	470, 2213, 2808
MlsI	TGGCCA	4 cut(s)	414, 617, 1014, 2389
MluNI	TGGCCA	4 cut(s)	414, 617, 1014, 2389
MlyI	GAGTC	1 cut(s)	947
MmeI	TCCRAC	1 cut(s)	483
Mox20I	TGGCCA	4 cut(s)	414, 617, 1014, 2389
MroI	TCCGGA	1 cut(s)	511
MroXI	GAANNNNTTC	3 cut(s)	782, 1257, 2448
MscI	TGGCCA	4 cut(s)	414, 617, 1014, 2389
MseI	TTAA	8 cut(s)	110, 122, 306, 327, 587, 906, 1700, 2753
MslI	CAYNNNNRTG	1 cut(s)	1502
Msp20I	TGGCCA	4 cut(s)	414, 617, 1014, 2389
MspA1I	CMGCKG	3 cut(s)	2143, 2826, 2867
MspI	CCGG	3 cut(s)	512, 920, 1640
MspR9I	CCNGG	5 cut(s)	180, 637, 753, 920, 984
MunI	CAATTG	2 cut(s)	204, 1281
Mva1269I	GAATGC	3 cut(s)	676, 920, 2011
MvaI	CCWGG	4 cut(s)	180, 637, 753, 984
MvnI	CGCG	3 cut(s)	1366, 2057, 2742
NciI	CCSGG	1 cut(s)	920
NdeI	CATATG	1 cut(s)	1189
NlaIV	GGNNCC	7 cut(s)	467, 1422, 1685, 1728, 1861, 1921, 2794
NmeAIII	GCCGAG	1 cut(s)	2770
NmuCI	GTSAC	2 cut(s)	1006, 1262
NspI	RCATGY	1 cut(s)	524
NspV	TTCGAA	1 cut(s)	2420
PagI	TCATGA	2 cut(s)	60, 1996
PceI	AGGCCT	1 cut(s)	751
PciSI	GCTCTTC	1 cut(s)	1600
PctI	GAATGC	3 cut(s)	676, 920, 2011
PdmI	GAANNNNTTC	3 cut(s)	782, 1257, 2448
PfeI	GAWTC	9 cut(s)	320, 719, 1166, 1300, 1412, 1586, 2378, 2552, 2614
Pfl23II	CGTACG	1 cut(s)	2495
PflFI	GACNNNGTC	1 cut(s)	990
PfoI	TCCNGGA	1 cut(s)	982
PleI	GAGTC	1 cut(s)	947
PpsI	GAGTC	1 cut(s)	947
PsiI	TTATAA	4 cut(s)	144, 162, 1745, 2832
Psp6I	CCWGG	4 cut(s)	178, 635, 751, 982
PspGI	CCWGG	4 cut(s)	178, 635, 751, 982
PspLI	CGTACG	1 cut(s)	2495
PspN4I	GGNNCC	7 cut(s)	467, 1422, 1685, 1728, 1861, 1921, 2794
PspPI	GGNCC	3 cut(s)	67, 421, 970
PstI	CTGCAG	3 cut(s)	887, 1609, 2590
PstNI	CAGNNNCTG	3 cut(s)	497, 983, 2826
PsuI	RGATCY	4 cut(s)	250, 802, 871, 1510
PsyI	GACNNNGTC	1 cut(s)	990
PvuII	CAGCTG	1 cut(s)	2867
RsaI	GTAC	8 cut(s)	702, 1022, 1496, 1652, 2054, 2339, 2497, 2699
RsaNI	GTAC	8 cut(s)	701, 1021, 1495, 1651, 2053, 2338, 2496, 2698
RseI	CAYNNNNRTG	1 cut(s)	1502
SapI	GCTCTTC	1 cut(s)	1600
SaqAI	TTAA	8 cut(s)	110, 122, 306, 327, 587, 906, 1700, 2753
Sau96I	GGNCC	3 cut(s)	67, 421, 970
SchI	GAGTC	1 cut(s)	947
ScrFI	CCNGG	5 cut(s)	180, 637, 753, 920, 984
SduI	GDGCHC	3 cut(s)	470, 2213, 2808
SfaNI	GCATC	8 cut(s)	1267, 1474, 1550, 1681, 1950, 2515, 2590, 2756
SfcI	CTRYAG	7 cut(s)	439, 883, 1044, 1605, 2268, 2586, 2868
SfuI	TTCGAA	1 cut(s)	2420
SinI	GGWCC	3 cut(s)	67, 421, 970
SmiMI	CAYNNNNRTG	1 cut(s)	1502
SmlI	CTYRAG	3 cut(s)	2453, 2634, 2784
SmoI	CTYRAG	3 cut(s)	2453, 2634, 2784
SseBI	AGGCCT	1 cut(s)	751
SsiI	CCGC	6 cut(s)	1106, 1345, 1366, 2143, 2684, 2824
SspI	AATATT	4 cut(s)	903, 1117, 1218, 2292
SspMI	CTAG	5 cut(s)	1341, 1887, 2226, 2517, 2568
StuI	AGGCCT	1 cut(s)	751
StyD4I	CCNGG	5 cut(s)	178, 635, 751, 918, 982
StyI	CCWWGG	1 cut(s)	2529
TaaI	ACNGT	6 cut(s)	304, 705, 1822, 2090, 2245, 2853
TaiI	ACGT	1 cut(s)	1806
TaqI	TCGA	7 cut(s)	244, 527, 1410, 1809, 2420, 2617, 2706
TatI	WGTACW	2 cut(s)	700, 2337
TauI	GCSGC	3 cut(s)	1347, 1369, 2826
TfiI	GAWTC	9 cut(s)	320, 719, 1166, 1300, 1412, 1586, 2378, 2552, 2614
Tru1I	TTAA	8 cut(s)	110, 122, 306, 327, 587, 906, 1700, 2753
Tru9I	TTAA	8 cut(s)	110, 122, 306, 327, 587, 906, 1700, 2753
TseFI	GTSAC	2 cut(s)	1006, 1262
TseI	GCWGC	8 cut(s)	281, 392, 656, 1604, 2252, 2360, 2588, 2820
Tsp45I	GTSAC	2 cut(s)	1006, 1262
TspGWI	ACGGA	6 cut(s)	62, 965, 1038, 1831, 1922, 2513
Tth111I	GACNNNGTC	1 cut(s)	990
VpaK11BI	GGWCC	3 cut(s)	67, 421, 970
XapI	RAATTY	8 cut(s)	48, 287, 1926, 2074, 2167, 2366, 2580, 2619
XbaI	TCTAGA	1 cut(s)	2567
XceI	RCATGY	1 cut(s)	524
XcmI	CCANNNNNNNNNTGG	2 cut(s)	550, 980
XmiI	GTMKAC	1 cut(s)	2535
XmnI	GAANNNNTTC	3 cut(s)	782, 1257, 2448
XspI	CTAG	5 cut(s)	1341, 1887, 2226, 2517, 2568

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.