pycom16g14830

RING-variant domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0005136
Physical Location & Seq
Reverse (-)
10864944 .. 10868887
3944 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1287 bp
ATGCTAGCTGCTGAATCAGTCCCCAATGGAGCTGCAGAACCTCCTCCTTCTCCTCAGGCTGGTTTCCAAAATCTGAAGACAGTAAATGAAGAAGCTACCGACCTGGAACCTACTGGGAGGCGCCCAGACATATCACTTCAGATACCCCGAAGGCCTGTGGGTTTTGGCAGTAGCTGTAGCGGAAAGGGTTTACTGCAGTCTCAAGGTTCCTTTAAGGGCAGCTCGCTATCAGGGGGCCTTTTCCGGGCTTTAAGCTTCAAGAGAAAAGGCAATCAACCGGATGGTGAGAGAAGCTCCCTCCTTAATTCAGACCCCAGTAGAGATCCTGAAAGATGTACCTCTCTTCCTGTTACACCGATATCAAATTTGTCCGCCTCTGTTGCTACACCCAGCTCTGCGAGGATGTATAATGAAGTGCACAAGCCACGTGAAGTCATCCAACTGAAAGAGATGGTTGTGATTAATTGTGTGCAGAAAGAAATTACAACAGTTTTGAGGTCCCTTTCTATGCCTGGATGTAATAAAGTCATTGTAAGATCCGTATCTTTTGCAACTCGAAATGAGCAGACTCAAGCAGATCCTAGTGATGATCAAATAACTCCAGTTCCAGTGGAGGCCAATGATGAAGAAATTCCTGAAGAAGAAGCAGTGTGCAGGATATGTCTTGATGCATGTGATGAACAAAATACGCTCAAAATGGAATGTTTTTGCAAAGGTGCTCTTAGACTTCTACATGAAGAGTGCGCCATTAAGTGGTTCAGCACAAAAGGAAACAAGAAATGTGATGTATGTGGTCAAGAAGTTCAGAATTTACCTGTAACATTGCTTCGGGTGCAGAGCACTGCTCAAAGGGGTAGTGGACAGCAGCACAACCAACACGGTTTGCATCCAGAAACGATAAGTGCCTGGCAGGACTTTGTGGTACTGGTCTTGATTAGCACAATATGCTATTTCTTCTTCCTTGAGCAGCTACTGATTCGTGACTTGAAAACTAGAGCGATTGTAATTACAGCACCGTTTGCATTTACATTGGGTCTCTTGGCATCCACATTCGCCATCATCCTTGCAATCAGGGAGTACATATGGACATATGCAGCTCTGGAGTTTGCGCTTGTCGCCCTCATCGTCCACCTGTTCTACAGCATACTTCATCTGATGGCCATTTACGCGATATTGCTTGCTGCGGTGTTGGGTTTCGGAATAGCGATTAGCCTCAACTCATTGTACATGAAATATTTCACCTGGAGAGTTCAAGTTGCACAATCTCAAAGCTCTAGCCCTGTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RINGv

Protein Analysis

429

Amino Acids

47.07

Weight (kDa)

6.41

Isoelectric Point (pI)

55.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RINGv PF12906 218 - 264 1.2e-12 RING-variant domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 120
AccB7I CCANNNNNTGG 1 cut(s) 753
AccII CGCG 1 cut(s) 1169
AciI CCGC 3 cut(s) 180, 372, 1184
AclWI GGATC 3 cut(s) 317, 531, 572
AcoI YGGCCR 1 cut(s) 1158
AcsI RAATTY 3 cut(s) 364, 630, 808
AcuI CTGAAG 3 cut(s) 95, 122, 657
AcvI CACGTG 1 cut(s) 428
AcyI GRCGYC 1 cut(s) 121
AfaI GTAC 4 cut(s) 337, 924, 1079, 1226
AfiI CCNNNNNNNGG 3 cut(s) 59, 244, 753
AgsI TTSAA 3 cut(s) 259, 988, 1253
AjnI CCWGG 4 cut(s) 102, 511, 905, 1241
Alw21I GWGCWC 3 cut(s) 420, 721, 842
Alw26I GTCTC 2 cut(s) 204, 1040
Alw44I GTGCAC 1 cut(s) 416
AlwI GGATC 3 cut(s) 317, 531, 572
AlwNI CAGNNNCTG 2 cut(s) 174, 973
AoxI GGCC 4 cut(s) 152, 235, 615, 1158
ApaLI GTGCAC 1 cut(s) 416
ApeKI GCWGC 7 cut(s) 8, 32, 219, 865, 967, 1094, 1181
ApoI RAATTY 3 cut(s) 364, 630, 808
AseI ATTAAT 1 cut(s) 462
Asp700I GAANNNNTTC 2 cut(s) 630, 1235
AspLEI GCGC 3 cut(s) 123, 746, 1111
AspS9I GGNCC 2 cut(s) 235, 498
AsuC2I CCSGG 1 cut(s) 245
AsuHPI GGTGA 2 cut(s) 296, 1231
AsuNHI GCTAGC 1 cut(s) 4
AvaII GGWCC 1 cut(s) 498
AxyI CCTNAGG 1 cut(s) 54
BaeGI GKGCMC 1 cut(s) 420
BalI TGGCCA 1 cut(s) 1160
BanI GGYRCC 1 cut(s) 120
BarI GAAGNNNNNNTAC 2 cut(s) 810, 842
BbrPI CACGTG 1 cut(s) 428
BbsI GAAGAC 1 cut(s) 83
Bbv12I GWGCWC 3 cut(s) 420, 721, 842
BbvI GCAGC 6 cut(s) 19, 231, 877, 979, 1106, 1168
BccI CCATC 4 cut(s) 275, 445, 1064, 1150
BciT130I CCWGG 4 cut(s) 104, 513, 907, 1243
BclI TGATCA 1 cut(s) 589
BcnI CCSGG 1 cut(s) 245
BcoDI GTCTC 2 cut(s) 204, 1040
BfaI CTAG 4 cut(s) 5, 582, 993, 1275
BfmI CTRYAG 4 cut(s) 33, 175, 194, 1138
BfoI RGCGCY 1 cut(s) 124
BisI GCNGC 7 cut(s) 9, 33, 220, 866, 968, 1095, 1182
BlsI GCNGC 7 cut(s) 10, 34, 221, 867, 969, 1096, 1183
Bme1390I CCNGG 5 cut(s) 104, 245, 513, 907, 1243
Bme18I GGWCC 1 cut(s) 498
BmgT120I GGNCC 2 cut(s) 235, 498
BmiI GGNNCC 5 cut(s) 108, 122, 208, 236, 500
BmrFI CCNGG 5 cut(s) 104, 245, 513, 907, 1243
BmrI ACTGGG 2 cut(s) 123, 309
BmsI GCATC 3 cut(s) 658, 895, 1052
BmtI GCTAGC 1 cut(s) 8
BmuI ACTGGG 2 cut(s) 123, 309
BpiI GAAGAC 1 cut(s) 83
BplI GAGNNNNNCTC 4 cut(s) 278, 310, 829, 861
BpmI CTGGAG 3 cut(s) 585, 1121, 1264
BpuEI CTTGAG 3 cut(s) 186, 555, 983
BpuMI CCSGG 1 cut(s) 245
BsaAI YACGTR 1 cut(s) 428
BsaBI GATNNNNATC 1 cut(s) 541
BsaHI GRCGYC 1 cut(s) 121
BsaI GGTCTC 1 cut(s) 1040
BsaWI WCCGGW 1 cut(s) 277
Bsc4I CCNNNNNNNGG 3 cut(s) 59, 244, 753
Bse1I ACTGG 5 cut(s) 118, 315, 602, 608, 930
Bse21I CCTNAGG 1 cut(s) 54
Bse3DI GCAATG 1 cut(s) 821
Bse8I GATNNNNATC 1 cut(s) 541
BseBI CCWGG 4 cut(s) 104, 513, 907, 1243
BseGI GGATG 7 cut(s) 286, 408, 435, 521, 886, 1043, 1059
BseJI GATNNNNATC 1 cut(s) 541
BseLI CCNNNNNNNGG 3 cut(s) 59, 244, 753
BseMI GCAATG 1 cut(s) 821
BseMII CTCAG 1 cut(s) 68
BseNI ACTGG 5 cut(s) 118, 315, 602, 608, 930
BseRI GAGGAG 2 cut(s) 33, 42
BseSI GKGCMC 1 cut(s) 420
BseXI GCAGC 6 cut(s) 19, 231, 877, 979, 1106, 1168
BseYI CCCAGC 1 cut(s) 389
BsgI GTGCAG 3 cut(s) 491, 673, 854
Bsh1236I CGCG 1 cut(s) 1169
BshFI GGCC 4 cut(s) 154, 237, 617, 1160
BshNI GGYRCC 1 cut(s) 120
BsiHKAI GWGCWC 3 cut(s) 420, 721, 842
BsiSI CCGG 2 cut(s) 244, 278
BslFI GGGAC 2 cut(s) 5, 484
BslI CCNNNNNNNGG 3 cut(s) 59, 244, 753
BsmAI GTCTC 2 cut(s) 204, 1040
BsmFI GGGAC 2 cut(s) 5, 484
BsnI GGCC 4 cut(s) 154, 237, 617, 1160
Bso31I GGTCTC 1 cut(s) 1040
Bsp1286I GDGCHC 3 cut(s) 420, 721, 842
Bsp1407I TGTACA 1 cut(s) 1224
Bsp143I GATC 4 cut(s) 322, 536, 577, 589
BspACI CCGC 3 cut(s) 180, 372, 1184
BspANI GGCC 4 cut(s) 154, 237, 617, 1160
BspCNI CTCAG 1 cut(s) 67
BspFNI CGCG 1 cut(s) 1169
BspLI GGNNCC 5 cut(s) 108, 122, 208, 236, 500
BspMAI CTGCAG 2 cut(s) 37, 198
BspOI GCTAGC 1 cut(s) 8
BspPI GGATC 3 cut(s) 317, 531, 572
BspT107I GGYRCC 1 cut(s) 120
BspTNI GGTCTC 1 cut(s) 1040
BsrDI GCAATG 1 cut(s) 821
BsrGI TGTACA 1 cut(s) 1224
BsrI ACTGG 5 cut(s) 118, 315, 602, 608, 930
BssMI GATC 4 cut(s) 322, 536, 577, 589
BssNI GRCGYC 1 cut(s) 121
Bst2UI CCWGG 4 cut(s) 104, 513, 907, 1243
Bst4CI ACNGT 4 cut(s) 82, 490, 881, 1017
Bst6I CTCTTC 2 cut(s) 348, 732
BstACI GRCGYC 1 cut(s) 121
BstAPI GCANNNNNTGC 2 cut(s) 945, 1019
BstAUI TGTACA 1 cut(s) 1224
BstBAI YACGTR 1 cut(s) 428
BstC8I GCNNGC 3 cut(s) 6, 224, 1179
BstDEI CTNAG 2 cut(s) 54, 722
BstF5I GGATG 7 cut(s) 286, 408, 435, 521, 886, 1043, 1059
BstFNI CGCG 1 cut(s) 1169
BstH2I RGCGCY 1 cut(s) 124
BstHHI GCGC 3 cut(s) 123, 746, 1111
BstKTI GATC 4 cut(s) 325, 539, 580, 592
BstMAI GTCTC 2 cut(s) 204, 1040
BstMBI GATC 4 cut(s) 322, 536, 577, 589
BstMWI GCNNNNNNNGC 6 cut(s) 380, 832, 945, 1019, 1115, 1166
BstNI CCWGG 4 cut(s) 104, 513, 907, 1243
BstNSI RCATGY 1 cut(s) 675
BstSCI CCNGG 5 cut(s) 102, 243, 511, 905, 1241
BstSFI CTRYAG 4 cut(s) 33, 175, 194, 1138
BstSLI GKGCMC 1 cut(s) 420
BstUI CGCG 1 cut(s) 1169
BstV1I GCAGC 6 cut(s) 19, 231, 877, 979, 1106, 1168
BstV2I GAAGAC 1 cut(s) 83
BstX2I RGATCY 3 cut(s) 322, 536, 577
BstYI RGATCY 3 cut(s) 322, 536, 577
Bsu36I CCTNAGG 1 cut(s) 54
BsuRI GGCC 4 cut(s) 154, 237, 617, 1160
BtsCI GGATG 7 cut(s) 286, 408, 435, 521, 886, 1043, 1059
BtsI GCAGTG 2 cut(s) 654, 840
BtsIMutI CAGTG 3 cut(s) 615, 654, 840
Cac8I GCNNGC 3 cut(s) 6, 224, 1179
CaiI CAGNNNCTG 2 cut(s) 174, 973
CfoI GCGC 3 cut(s) 123, 746, 1111
Cfr13I GGNCC 2 cut(s) 235, 498
Csp6I GTAC 4 cut(s) 336, 923, 1078, 1225
CviAII CATG 3 cut(s) 672, 734, 1228
CviQI GTAC 4 cut(s) 336, 923, 1078, 1225
DdeI CTNAG 2 cut(s) 54, 722
DinI GGCGCC 1 cut(s) 122
DpnI GATC 4 cut(s) 324, 538, 579, 591
DpnII GATC 4 cut(s) 322, 536, 577, 589
EaeI YGGCCR 1 cut(s) 1158
Eam1104I CTCTTC 2 cut(s) 348, 732
EarI CTCTTC 2 cut(s) 348, 732
EciI GGCGGA 1 cut(s) 361
Eco147I AGGCCT 1 cut(s) 154
Eco31I GGTCTC 1 cut(s) 1040
Eco32I GATATC 1 cut(s) 360
Eco47I GGWCC 1 cut(s) 498
Eco57I CTGAAG 3 cut(s) 95, 122, 657
Eco72I CACGTG 1 cut(s) 428
Eco81I CCTNAGG 1 cut(s) 54
EcoO109I RGGNCCY 2 cut(s) 235, 498
EcoRII CCWGG 4 cut(s) 102, 511, 905, 1241
EcoRV GATATC 1 cut(s) 360
EcoT22I ATGCAT 1 cut(s) 673
EgeI GGCGCC 1 cut(s) 122
EheI GGCGCC 1 cut(s) 122
FaeI CATG 3 cut(s) 675, 737, 1231
FalI AAGNNNNNCTT 2 cut(s) 705, 737
FaqI GGGAC 2 cut(s) 5, 484
FatI CATG 3 cut(s) 671, 733, 1227
FauNDI CATATG 2 cut(s) 1082, 1090
FbaI TGATCA 1 cut(s) 589
Fnu4HI GCNGC 7 cut(s) 9, 33, 220, 866, 968, 1095, 1182
FokI GGATG 7 cut(s) 293, 415, 422, 528, 873, 1030, 1046
Fsp4HI GCNGC 7 cut(s) 9, 33, 220, 866, 968, 1095, 1182
FspBI CTAG 4 cut(s) 5, 582, 993, 1275
GlaI GCGC 3 cut(s) 122, 745, 1110
GluI GCNGC 7 cut(s) 9, 33, 220, 866, 968, 1095, 1182
GsaI CCCAGC 1 cut(s) 393
GsuI CTGGAG 3 cut(s) 585, 1121, 1264
HaeII RGCGCY 1 cut(s) 124
HaeIII GGCC 4 cut(s) 154, 237, 617, 1160
HapII CCGG 2 cut(s) 244, 278
HhaI GCGC 3 cut(s) 123, 746, 1111
Hin1I GRCGYC 1 cut(s) 121
Hin1II CATG 3 cut(s) 675, 737, 1231
Hin6I GCGC 3 cut(s) 121, 744, 1109
HinP1I GCGC 3 cut(s) 121, 744, 1109
HindIII AAGCTT 1 cut(s) 253
HinfI GANTC 3 cut(s) 14, 568, 976
HpaII CCGG 2 cut(s) 244, 278
HphI GGTGA 2 cut(s) 296, 1231
Hpy166II GTNNAC 4 cut(s) 191, 418, 860, 1129
Hpy188I TCNGA 6 cut(s) 75, 141, 310, 807, 1155, 1199
Hpy188III TCNNGA 9 cut(s) 259, 326, 635, 665, 797, 890, 931, 980, 1100
Hpy8I GTNNAC 4 cut(s) 191, 418, 860, 1129
HpyAV CCTTC 2 cut(s) 57, 144
HpyCH4III ACNGT 4 cut(s) 82, 490, 881, 1017
HpyCH4IV ACGT 1 cut(s) 427
HpyF10VI GCNNNNNNNGC 6 cut(s) 380, 832, 945, 1019, 1115, 1166
HpyF3I CTNAG 2 cut(s) 54, 722
HpySE526I ACGT 1 cut(s) 427
Hsp92I GRCGYC 1 cut(s) 121
Hsp92II CATG 3 cut(s) 675, 737, 1231
HspAI GCGC 3 cut(s) 121, 744, 1109
KasI GGCGCC 1 cut(s) 120
Ksp22I TGATCA 1 cut(s) 589
Kzo9I GATC 4 cut(s) 322, 536, 577, 589
LmnI GCTCC 2 cut(s) 29, 299
Lsp1109I GCAGC 6 cut(s) 19, 231, 877, 979, 1106, 1168
LweI GCATC 3 cut(s) 658, 895, 1052
MaeI CTAG 4 cut(s) 5, 582, 993, 1275
MaeII ACGT 1 cut(s) 427
MaeIII GTNAC 3 cut(s) 349, 817, 980
MalI GATC 4 cut(s) 324, 538, 579, 591
MboI GATC 4 cut(s) 322, 536, 577, 589
MboII GAAGA 9 cut(s) 88, 101, 335, 638, 650, 653, 749, 946, 949
MflI RGATCY 3 cut(s) 322, 536, 577
MhlI GDGCHC 3 cut(s) 420, 721, 842
MlsI TGGCCA 1 cut(s) 1160
MluCI AATT 7 cut(s) 304, 364, 463, 480, 630, 808, 1005
MluNI TGGCCA 1 cut(s) 1160
Mly113I GGCGCC 1 cut(s) 121
MlyI GAGTC 1 cut(s) 562
MmeI TCCRAC 1 cut(s) 463
Mox20I TGGCCA 1 cut(s) 1160
Mph1103I ATGCAT 1 cut(s) 673
MroXI GAANNNNTTC 2 cut(s) 630, 1235
MscI TGGCCA 1 cut(s) 1160
MseI TTAA 5 cut(s) 213, 251, 303, 462, 750
Msp20I TGGCCA 1 cut(s) 1160
MspI CCGG 2 cut(s) 244, 278
MspR9I CCNGG 5 cut(s) 104, 245, 513, 907, 1243
MvaI CCWGG 4 cut(s) 104, 513, 907, 1243
MvnI CGCG 1 cut(s) 1169
MwoI GCNNNNNNNGC 6 cut(s) 380, 832, 945, 1019, 1115, 1166
NarI GGCGCC 1 cut(s) 121
NciI CCSGG 1 cut(s) 245
NdeI CATATG 2 cut(s) 1082, 1090
NdeII GATC 4 cut(s) 322, 536, 577, 589
NheI GCTAGC 1 cut(s) 4
NlaIII CATG 3 cut(s) 675, 737, 1231
NlaIV GGNNCC 5 cut(s) 108, 122, 208, 236, 500
NmuCI GTSAC 1 cut(s) 980
NsiI ATGCAT 1 cut(s) 673
NspI RCATGY 1 cut(s) 675
PceI AGGCCT 1 cut(s) 154
PcsI WCGNNNNNNNCGW 1 cut(s) 1122
PdmI GAANNNNTTC 2 cut(s) 630, 1235
PfeI GAWTC 2 cut(s) 14, 976
PflMI CCANNNNNTGG 1 cut(s) 753
PkrI GCNGC 7 cut(s) 10, 34, 221, 867, 969, 1096, 1183
PleI GAGTC 1 cut(s) 562
PluTI GGCGCC 1 cut(s) 124
PmaCI CACGTG 1 cut(s) 428
PmlI CACGTG 1 cut(s) 428
PpsI GAGTC 1 cut(s) 562
Ppu21I YACGTR 1 cut(s) 428
PpuMI RGGWCCY 1 cut(s) 498
PshBI ATTAAT 1 cut(s) 462
Psp5II RGGWCCY 1 cut(s) 498
Psp6I CCWGG 4 cut(s) 102, 511, 905, 1241
PspCI CACGTG 1 cut(s) 428
PspFI CCCAGC 1 cut(s) 389
PspGI CCWGG 4 cut(s) 102, 511, 905, 1241
PspN4I GGNNCC 5 cut(s) 108, 122, 208, 236, 500
PspPI GGNCC 2 cut(s) 235, 498
PspPPI RGGWCCY 1 cut(s) 498
PstI CTGCAG 2 cut(s) 37, 198
PstNI CAGNNNCTG 2 cut(s) 174, 973
PsuI RGATCY 3 cut(s) 322, 536, 577
RsaI GTAC 4 cut(s) 337, 924, 1079, 1226
RsaNI GTAC 4 cut(s) 336, 923, 1078, 1225
SaqAI TTAA 5 cut(s) 213, 251, 303, 462, 750
SatI GCNGC 7 cut(s) 9, 33, 220, 866, 968, 1095, 1182
Sau3AI GATC 4 cut(s) 322, 536, 577, 589
Sau96I GGNCC 2 cut(s) 235, 498
SchI GAGTC 1 cut(s) 562
ScrFI CCNGG 5 cut(s) 104, 245, 513, 907, 1243
SduI GDGCHC 3 cut(s) 420, 721, 842
SfaNI GCATC 3 cut(s) 658, 895, 1052
SfcI CTRYAG 4 cut(s) 33, 175, 194, 1138
SfoI GGCGCC 1 cut(s) 122
SinI GGWCC 1 cut(s) 498
SmlI CTYRAG 3 cut(s) 201, 570, 962
SmoI CTYRAG 3 cut(s) 201, 570, 962
Sse9I AATT 7 cut(s) 304, 364, 463, 480, 630, 808, 1005
SseBI AGGCCT 1 cut(s) 154
SsiI CCGC 3 cut(s) 180, 372, 1184
SspDI GGCGCC 1 cut(s) 120
SspI AATATT 1 cut(s) 1235
SspMI CTAG 4 cut(s) 5, 582, 993, 1275
StuI AGGCCT 1 cut(s) 154
StyD4I CCNGG 5 cut(s) 102, 243, 511, 905, 1241
TaaI ACNGT 4 cut(s) 82, 490, 881, 1017
TaiI ACGT 1 cut(s) 430
TaqI TCGA 1 cut(s) 556
TasI AATT 7 cut(s) 304, 364, 463, 480, 630, 808, 1005
TatI WGTACW 2 cut(s) 1077, 1224
TfiI GAWTC 2 cut(s) 14, 976
Tru1I TTAA 5 cut(s) 213, 251, 303, 462, 750
Tru9I TTAA 5 cut(s) 213, 251, 303, 462, 750
TscAI CASTG 3 cut(s) 615, 654, 847
TseFI GTSAC 1 cut(s) 980
TseI GCWGC 7 cut(s) 8, 32, 219, 865, 967, 1094, 1181
Tsp45I GTSAC 1 cut(s) 980
TspDTI ATGAA 7 cut(s) 102, 426, 639, 693, 750, 1139, 1244
TspGWI ACGGA 1 cut(s) 529
TspRI CASTG 3 cut(s) 615, 654, 847
Van91I CCANNNNNTGG 1 cut(s) 753
VneI GTGCAC 1 cut(s) 416
VpaK11BI GGWCC 1 cut(s) 498
VspI ATTAAT 1 cut(s) 462
XapI RAATTY 3 cut(s) 364, 630, 808
XceI RCATGY 1 cut(s) 675
XmnI GAANNNNTTC 2 cut(s) 630, 1235
XspI CTAG 4 cut(s) 5, 582, 993, 1275
Zsp2I ATGCAT 1 cut(s) 673
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.