pycom16g15150

calcium-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0009246
Physical Location & Seq
Forward (+)
11160091 .. 11163753
3663 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1026 bp
ATGTCGTTTTCGTTCTTCAAGCCGTCGAGGCCCAAAACCCCTCAGGAGGTGGCCAAGGCCATCAACGACAGCCTCCTGGCCCTCGACATCCAGACCGTTGTCGAAGTCAAAGCCCTCGAAAAGGCTTTGGAAGAGGTTGAAAAGAATTTCACGACAATGAAATGTATGCTTTCTGGAGATGGGGAGACCGAACCAAATATGGATCACATTTCACAGCTTGCTCTTGAGATTTGTAAGGAGGGTGTTCTTGATCTTCTAATTCACAAGTTACCTATACTGGGATGGGAAGCAAGAAAGGATTTGGTCCATTGTTGGTCCATATTGATGAAGCAAAAGGTTGAATCGACTTATTGCTGTGCAGAATACATGGAGAACCATTTGGAATTATTAGACTTTCTTGTCGTGTACTATGATAACAAGGAAATTGCTTTGAATTGTGGGAATATGTTGAGGGATTGCATTAGATTCCCTGCACTTGCAAAATACATACTGGAGTCTGCGAGCTTTGAGTTGTTCTTCAAATATGTGGAGTTGCCTAACTTTGACGTTGCTTCTGATGCATTCTCTACCTTTAAGGATCTACTTACTAAACATGGAACAGTGGTATCTGAATTTTTGACTGCTCACTATGATGAGTTCTTTGATCTATATGAAAAGCTCTTGACATCTCCAAATTATGTGACCAGGAGGCAATCTTTGAAGCTTCTCTCCGAATTCCTCTTGGAGACTCCAAATTCACATATAATGAAGCGCTACATTTTAGAAGTTCGGTACCTGAAAGTCATGATGACGTTGCTCAAGGATTCAAGTAAAAACATCCAAATCTCTGCCTTCCACATCTTCAAGGTCTTTGTTGCGAATCCTAATAAGCCACGGGAAGTAAAACTTATCCTGGCTAAAAACCATGAAAAGTTGACGGACTTGCTTCAGAATCTTTCTGCTGGAAAAGGTGATGAAGACGAGCAATTTGAAGAGGAAAAGGAGCTGATCGATCAAGGAAATCGAAAGAGTATCTCAACTAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04150 ko04152 map04150 map04152
Pfam Domains
Protein Families
Mo25

Protein Analysis

342

Amino Acids

39.34

Weight (kDa)

5.42

Isoelectric Point (pI)

42.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mo25 PF08569 5 - 330 1.7e-123 Mo25-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 398
Acc65I GGTACC 1 cut(s) 771
AccB1I GGYRCC 1 cut(s) 771
AclWI GGATC 2 cut(s) 210, 585
AcoI YGGCCR 1 cut(s) 51
AcsI RAATTY 4 cut(s) 145, 611, 713, 733
AcuI CTGAAG 1 cut(s) 911
AfaI GTAC 2 cut(s) 407, 773
AfeI AGCGCT 1 cut(s) 752
AfiI CCNNNNNNNGG 3 cut(s) 46, 121, 278
AgsI TTSAA 9 cut(s) 19, 140, 341, 433, 520, 700, 807, 844, 971
AhlI ACTAGT 1 cut(s) 1019
AjnI CCWGG 3 cut(s) 75, 683, 891
AluBI AGCT 5 cut(s) 217, 504, 658, 703, 985
AluI AGCT 5 cut(s) 217, 504, 658, 703, 985
Alw26I GTCTC 2 cut(s) 179, 719
AlwI GGATC 2 cut(s) 210, 585
Aor51HI AGCGCT 1 cut(s) 752
AoxI GGCC 4 cut(s) 29, 51, 57, 78
ApoI RAATTY 4 cut(s) 145, 611, 713, 733
ArsI GACNNNNNNTTYG 2 cut(s) 950, 982
Asp718I GGTACC 1 cut(s) 771
AspLEI GCGC 1 cut(s) 753
AspS9I GGNCC 4 cut(s) 30, 79, 304, 315
AsuHPI GGTGA 1 cut(s) 962
AvaII GGWCC 2 cut(s) 304, 315
AxyI CCTNAGG 1 cut(s) 42
BaeI ACNNNNGTAYC 2 cut(s) 588, 621
BalI TGGCCA 1 cut(s) 53
BanI GGYRCC 1 cut(s) 771
BbsI GAAGAC 1 cut(s) 963
BccI CCATC 3 cut(s) 68, 173, 276
BceAI ACGGC 1 cut(s) 7
BciT130I CCWGG 3 cut(s) 77, 685, 893
BcoDI GTCTC 2 cut(s) 179, 719
BcuI ACTAGT 1 cut(s) 1019
BfaI CTAG 1 cut(s) 1020
BfoI RGCGCY 1 cut(s) 754
BglI GCCNNNNNGGC 1 cut(s) 28
Bme1390I CCNGG 3 cut(s) 77, 685, 893
Bme18I GGWCC 2 cut(s) 304, 315
BmgT120I GGNCC 4 cut(s) 30, 79, 304, 315
BmiI GGNNCC 1 cut(s) 773
BmrFI CCNGG 3 cut(s) 77, 685, 893
BmrI ACTGGG 1 cut(s) 287
BmsI GCATC 1 cut(s) 547
BmuI ACTGGG 1 cut(s) 287
BoxI GACNNNNGTC 1 cut(s) 98
BpiI GAAGAC 1 cut(s) 963
BpmI CTGGAG 2 cut(s) 195, 512
BpuEI CTTGAG 2 cut(s) 245, 782
Bsa29I ATCGAT 1 cut(s) 990
BsaI GGTCTC 1 cut(s) 179
BsaJI CCNNGG 2 cut(s) 54, 872
Bsc4I CCNNNNNNNGG 3 cut(s) 46, 121, 278
Bse1I ACTGG 2 cut(s) 282, 495
Bse21I CCTNAGG 1 cut(s) 42
BseBI CCWGG 3 cut(s) 77, 685, 893
BseCI ATCGAT 1 cut(s) 990
BseDI CCNNGG 2 cut(s) 54, 872
BseGI GGATG 3 cut(s) 87, 287, 816
BseLI CCNNNNNNNGG 3 cut(s) 46, 121, 278
BseMII CTCAG 1 cut(s) 56
BseNI ACTGG 2 cut(s) 282, 495
BsgI GTGCAG 2 cut(s) 378, 456
BshFI GGCC 4 cut(s) 31, 53, 59, 80
BshNI GGYRCC 1 cut(s) 771
BshVI ATCGAT 1 cut(s) 990
BslI CCNNNNNNNGG 3 cut(s) 46, 121, 278
BsmAI GTCTC 2 cut(s) 179, 719
BsmI GAATGC 1 cut(s) 560
BsnI GGCC 4 cut(s) 31, 53, 59, 80
Bso31I GGTCTC 1 cut(s) 179
Bsp143I GATC 6 cut(s) 202, 250, 577, 643, 987, 991
BspANI GGCC 4 cut(s) 31, 53, 59, 80
BspCNI CTCAG 1 cut(s) 55
BspDI ATCGAT 1 cut(s) 990
BspHI TCATGA 1 cut(s) 783
BspLI GGNNCC 1 cut(s) 773
BspPI GGATC 2 cut(s) 210, 585
BspT107I GGYRCC 1 cut(s) 771
BspTNI GGTCTC 1 cut(s) 179
BsrI ACTGG 2 cut(s) 282, 495
BssECI CCNNGG 2 cut(s) 54, 872
BssMI GATC 6 cut(s) 202, 250, 577, 643, 987, 991
BssT1I CCWWGG 1 cut(s) 54
Bst2UI CCWGG 3 cut(s) 77, 685, 893
Bst4CI ACNGT 2 cut(s) 97, 601
Bst6I CTCTTC 2 cut(s) 126, 966
BstC8I GCNNGC 2 cut(s) 219, 502
BstDEI CTNAG 1 cut(s) 42
BstDSI CCRYGG 1 cut(s) 872
BstENI CCTNNNNNAGG 1 cut(s) 119
BstF5I GGATG 3 cut(s) 87, 287, 816
BstH2I RGCGCY 1 cut(s) 754
BstHHI GCGC 1 cut(s) 753
BstKTI GATC 6 cut(s) 205, 253, 580, 646, 990, 994
BstMAI GTCTC 2 cut(s) 179, 719
BstMBI GATC 6 cut(s) 202, 250, 577, 643, 987, 991
BstMWI GCNNNNNNNGC 2 cut(s) 28, 557
BstNI CCWGG 3 cut(s) 77, 685, 893
BstPAI GACNNNNGTC 1 cut(s) 98
BstSCI CCNGG 3 cut(s) 75, 683, 891
BstV2I GAAGAC 1 cut(s) 963
BstX2I RGATCY 1 cut(s) 577
BstYI RGATCY 1 cut(s) 577
Bsu15I ATCGAT 1 cut(s) 990
Bsu36I CCTNAGG 1 cut(s) 42
BsuRI GGCC 4 cut(s) 31, 53, 59, 80
BsuTUI ATCGAT 1 cut(s) 990
BtgI CCRYGG 1 cut(s) 872
BtsCI GGATG 3 cut(s) 87, 287, 816
BtsIMutI CAGTG 1 cut(s) 606
Cac8I GCNNGC 2 cut(s) 219, 502
CciI TCATGA 1 cut(s) 783
CfoI GCGC 1 cut(s) 753
Cfr13I GGNCC 4 cut(s) 30, 79, 304, 315
ClaI ATCGAT 1 cut(s) 990
Csp6I GTAC 2 cut(s) 406, 772
CviAII CATG 4 cut(s) 367, 593, 784, 905
CviQI GTAC 2 cut(s) 406, 772
DdeI CTNAG 1 cut(s) 42
DpnI GATC 6 cut(s) 204, 252, 579, 645, 989, 993
DpnII GATC 6 cut(s) 202, 250, 577, 643, 987, 991
DrdI GACNNNNNNGTC 1 cut(s) 398
DseDI GACNNNNNNGTC 1 cut(s) 398
EaeI YGGCCR 1 cut(s) 51
Eam1104I CTCTTC 2 cut(s) 126, 966
EarI CTCTTC 2 cut(s) 126, 966
Eco130I CCWWGG 1 cut(s) 54
Eco31I GGTCTC 1 cut(s) 179
Eco47I GGWCC 2 cut(s) 304, 315
Eco47III AGCGCT 1 cut(s) 752
Eco57I CTGAAG 1 cut(s) 911
Eco81I CCTNAGG 1 cut(s) 42
EcoNI CCTNNNNNAGG 1 cut(s) 119
EcoRI GAATTC 1 cut(s) 713
EcoRII CCWGG 3 cut(s) 75, 683, 891
EcoT14I CCWWGG 1 cut(s) 54
EcoT22I ATGCAT 1 cut(s) 562
ErhI CCWWGG 1 cut(s) 54
FaeI CATG 4 cut(s) 370, 596, 787, 908
FalI AAGNNNNNCTT 2 cut(s) 870, 902
FatI CATG 4 cut(s) 366, 592, 783, 904
FokI GGATG 3 cut(s) 74, 294, 803
FspBI CTAG 1 cut(s) 1020
GlaI GCGC 1 cut(s) 752
GsuI CTGGAG 2 cut(s) 195, 512
HaeII RGCGCY 1 cut(s) 754
HaeIII GGCC 4 cut(s) 31, 53, 59, 80
HhaI GCGC 1 cut(s) 753
Hin1II CATG 4 cut(s) 370, 596, 787, 908
Hin6I GCGC 1 cut(s) 751
HinP1I GCGC 1 cut(s) 751
HincII GTYRAC 1 cut(s) 915
HindII GTYRAC 1 cut(s) 915
HindIII AAGCTT 1 cut(s) 701
HinfI GANTC 7 cut(s) 341, 465, 494, 727, 803, 859, 931
HphI GGTGA 1 cut(s) 962
Hpy166II GTNNAC 2 cut(s) 406, 915
Hpy188I TCNGA 4 cut(s) 556, 610, 712, 930
Hpy188III TCNNGA 8 cut(s) 44, 91, 151, 174, 224, 248, 661, 784
Hpy8I GTNNAC 2 cut(s) 406, 915
Hpy99I CGWCG 1 cut(s) 28
HpyAV CCTTC 1 cut(s) 841
HpyCH4III ACNGT 2 cut(s) 97, 601
HpyCH4IV ACGT 2 cut(s) 546, 791
HpyCH4V TGCA 5 cut(s) 359, 459, 473, 479, 560
HpyF10VI GCNNNNNNNGC 2 cut(s) 28, 557
HpyF3I CTNAG 1 cut(s) 42
HpySE526I ACGT 2 cut(s) 546, 791
Hsp92II CATG 4 cut(s) 370, 596, 787, 908
HspAI GCGC 1 cut(s) 751
KpnI GGTACC 1 cut(s) 775
Kzo9I GATC 6 cut(s) 202, 250, 577, 643, 987, 991
LmnI GCTCC 1 cut(s) 982
LweI GCATC 1 cut(s) 547
MaeI CTAG 1 cut(s) 1020
MaeII ACGT 2 cut(s) 546, 791
MaeIII GTNAC 2 cut(s) 267, 679
MalI GATC 6 cut(s) 204, 252, 579, 645, 989, 993
MboI GATC 6 cut(s) 202, 250, 577, 643, 987, 991
MboII GAAGA 7 cut(s) 7, 143, 245, 508, 832, 968, 983
MflI RGATCY 1 cut(s) 577
MlsI TGGCCA 1 cut(s) 53
MluNI TGGCCA 1 cut(s) 53
MlyI GAGTC 2 cut(s) 503, 721
Mox20I TGGCCA 1 cut(s) 53
Mph1103I ATGCAT 1 cut(s) 562
MscI TGGCCA 1 cut(s) 53
MseI TTAA 2 cut(s) 573, 1024
MslI CAYNNNNRTG 3 cut(s) 155, 323, 630
Msp20I TGGCCA 1 cut(s) 53
MspR9I CCNGG 3 cut(s) 77, 685, 893
Mva1269I GAATGC 1 cut(s) 560
MvaI CCWGG 3 cut(s) 77, 685, 893
MwoI GCNNNNNNNGC 2 cut(s) 28, 557
NdeII GATC 6 cut(s) 202, 250, 577, 643, 987, 991
NlaIII CATG 4 cut(s) 370, 596, 787, 908
NlaIV GGNNCC 1 cut(s) 773
NmuCI GTSAC 1 cut(s) 679
NsiI ATGCAT 1 cut(s) 562
PagI TCATGA 1 cut(s) 783
PctI GAATGC 1 cut(s) 560
PfeI GAWTC 5 cut(s) 341, 465, 803, 859, 931
PleI GAGTC 2 cut(s) 502, 721
PpsI GAGTC 2 cut(s) 502, 721
PshAI GACNNNNGTC 1 cut(s) 98
Psp6I CCWGG 3 cut(s) 75, 683, 891
PspGI CCWGG 3 cut(s) 75, 683, 891
PspN4I GGNNCC 1 cut(s) 773
PspPI GGNCC 4 cut(s) 30, 79, 304, 315
PsuI RGATCY 1 cut(s) 577
RsaI GTAC 2 cut(s) 407, 773
RsaNI GTAC 2 cut(s) 406, 772
RseI CAYNNNNRTG 3 cut(s) 155, 323, 630
SaqAI TTAA 2 cut(s) 573, 1024
Sau3AI GATC 6 cut(s) 202, 250, 577, 643, 987, 991
Sau96I GGNCC 4 cut(s) 30, 79, 304, 315
SchI GAGTC 2 cut(s) 503, 721
ScrFI CCNGG 3 cut(s) 77, 685, 893
SfaNI GCATC 1 cut(s) 547
SinI GGWCC 2 cut(s) 304, 315
SmiMI CAYNNNNRTG 3 cut(s) 155, 323, 630
SmlI CTYRAG 2 cut(s) 224, 797
SmoI CTYRAG 2 cut(s) 224, 797
SpeI ACTAGT 1 cut(s) 1019
SspMI CTAG 1 cut(s) 1020
StyD4I CCNGG 3 cut(s) 75, 683, 891
StyI CCWWGG 1 cut(s) 54
TaaI ACNGT 2 cut(s) 97, 601
TaiI ACGT 2 cut(s) 549, 794
TaqI TCGA 7 cut(s) 26, 84, 102, 117, 344, 990, 1003
TaqII GACCGA 1 cut(s) 203
TatI WGTACW 1 cut(s) 405
TfiI GAWTC 5 cut(s) 341, 465, 803, 859, 931
Tru1I TTAA 2 cut(s) 573, 1024
Tru9I TTAA 2 cut(s) 573, 1024
TscAI CASTG 1 cut(s) 606
TseFI GTSAC 1 cut(s) 679
Tsp45I GTSAC 1 cut(s) 679
TspDTI ATGAA 6 cut(s) 173, 341, 666, 761, 921, 969
TspGWI ACGGA 1 cut(s) 932
TspRI CASTG 1 cut(s) 606
VpaK11BI GGWCC 2 cut(s) 304, 315
XagI CCTNNNNNAGG 1 cut(s) 119
XapI RAATTY 4 cut(s) 145, 611, 713, 733
XspI CTAG 1 cut(s) 1020
Zsp2I ATGCAT 1 cut(s) 562
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.